Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,936	(C)_5→6	coding (846/2424 nt)	b1489 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,931	1	.	C	97.8%	561.4 / 4.4	181	coding (851/2424 nt)	b1489	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (88/88); total (90/91)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

ATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATAT  >  NC_000913/1562839‑1563018

aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t‑t                                                                                          >  1:3521270/1‑93 (MQ=255)

aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t‑t                                                                                          <  1:379441/93‑1 (MQ=255)

aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t‑t                                                                                          <  2:5981259/93‑1 (MQ=255)

  gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑cc                                                                                       >  1:5887840/1‑93 (MQ=255)

  gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑cc                                                                                       <  2:5181610/93‑1 (MQ=255)

   aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccc                                                                                      >  1:2786626/1‑93 (MQ=255)

   aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccc                                                                                      <  2:3218316/93‑1 (MQ=255)

     gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccccc                                                                                    <  2:2534305/93‑1 (MQ=255)

     gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccccc                                                                                    <  2:2955391/93‑1 (MQ=255)

     gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccccc                                                                                    >  2:3282169/1‑93 (MQ=255)

      cccAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑cccccc                                                                                   <  2:1792015/93‑2 (MQ=255)

      cccAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑cccccc                                                                                   >  2:3636467/1‑92 (MQ=255)

       ccAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑cccccca                                                                                  <  2:1593614/93‑3 (MQ=255)

        cAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑ccccccat                                                                                 >  1:3025059/1‑90 (MQ=255)

             tATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCAt                                                                             >  1:4146534/1‑93 (MQ=255)

             tATCGCCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCAt                                                                             <  1:1132747/93‑1 (MQ=255)

              aTCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATg                                                                            <  1:5379192/93‑1 (MQ=255)

               tCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGa                                                                           >  2:5281654/1‑93 (MQ=255)

                 ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGaaa                                                                         <  1:5934855/93‑1 (MQ=255)

                 ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGaaa                                                                         >  1:4199403/1‑93 (MQ=255)

                 ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGaaa                                                                         <  2:811852/93‑1 (MQ=255)

                  gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAAt                                                                        <  1:3282575/93‑1 (MQ=255)

                   cATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATg                                                                       <  2:2450193/93‑1 (MQ=255)

                   cATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATg                                                                       <  2:463025/93‑1 (MQ=255)

                    aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATgg                                                                      <  2:3268894/93‑1 (MQ=255)

                    aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATgg                                                                      >  2:3763313/1‑93 (MQ=255)

                    aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATgg                                                                      <  2:4780732/93‑1 (MQ=255)

                     tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGc                                                                     <  1:364800/93‑1 (MQ=255)

                     tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGc                                                                     <  2:4963844/93‑1 (MQ=255)

                     tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGc                                                                     >  2:1716085/1‑93 (MQ=255)

                     tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGc                                                                     <  2:2124632/93‑1 (MQ=255)

                     tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGc                                                                     >  1:749460/1‑93 (MQ=255)

                       ccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCgg                                                                   <  2:114236/93‑1 (MQ=255)

                       ccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCgg                                                                   >  1:5073642/1‑93 (MQ=255)

                         gTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGgc                                                                 <  2:299322/93‑1 (MQ=255)

                          ttGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGgct                                                                >  1:1821587/1‑93 (MQ=255)

                          ttGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGgct                                                                >  2:6240284/1‑93 (MQ=255)

                                aGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCtgctgcc                                                          >  1:375543/1‑92 (MQ=255)

                                aGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGca                                                          >  2:5928270/1‑93 (MQ=255)

                                aGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGca                                                          >  2:3904791/1‑93 (MQ=255)

                                aGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGca                                                          <  2:344336/93‑1 (MQ=255)

                                  tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGcaca                                                        <  1:1095395/93‑1 (MQ=255)

                                  tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGcaca                                                        >  1:423688/1‑93 (MQ=255)

                                   gCGAGCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAt                                                       <  2:92091/93‑1 (MQ=255)

                                   gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAt                                                       <  1:6072359/93‑1 (MQ=255)

                                   gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAt                                                       <  2:1448054/93‑1 (MQ=255)

                                    cGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATg                                                      <  1:2978395/93‑1 (MQ=255)

                                      aaCAGTGATGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATCG‑C‑CCCCCATTTCGTGATAGGGCGGGCTGCTCCACAGggc                                                    <  2:2367406/93‑1 (MQ=255)

                                       aCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggcg                                                   >  2:2737380/1‑93 (MQ=255)

                                        cAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggcgg                                                  <  1:6243614/93‑1 (MQ=255)

                                         aGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggcggc                                                 <  1:1742045/93‑1 (MQ=255)

                                          gCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGgcggcg                                                >  1:2164716/1‑93 (MQ=255)

                                           cGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCga                                               <  1:3877346/93‑1 (MQ=255)

                                               gcATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAGGGCGGCGAGAat                                           <  2:5014867/92‑1 (MQ=255)

                                                cATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAata                                          <  1:1015646/93‑1 (MQ=255)

                                                  tGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAtt                                        <  2:4377674/93‑1 (MQ=255)

                                                  tGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAtt                                        <  1:4371793/93‑1 (MQ=255)

                                                    cGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGc                                      <  1:5820255/93‑1 (MQ=255)

                                                     gATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGcc                                     >  2:1557163/1‑93 (MQ=255)

                                                     gATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGcc                                     <  1:1296945/93‑1 (MQ=255)

                                                      aTTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCt                                    <  1:1385803/93‑1 (MQ=255)

                                                       ttCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCtt                                   >  1:1608707/1‑93 (MQ=255)

                                                        tCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTc                                  <  1:1534725/93‑1 (MQ=255)

                                                        tCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTc                                  <  1:209054/93‑1 (MQ=255)

                                                         cGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGGACATGGCGGCGAGAATATTGCCTTCa                                 >  2:5801869/1‑93 (MQ=255)

                                                         cGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCa                                 <  1:2591070/93‑1 (MQ=255)

                                                          cttGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCaa                                <  1:1182623/92‑1 (MQ=255)

                                                            tGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGc                              <  1:4718780/93‑1 (MQ=255)

                                                            tGAGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTAAAGc                              <  2:3404728/93‑1 (MQ=255)

                                                             gAGTACAGATGCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCAGATGGCGGCGAGAATATTGCCTTCAAGCt                             <  2:2577340/93‑1 (MQ=255)

                                                              aGTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTg                            <  1:4943283/93‑1 (MQ=255)

                                                               gTACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTgg                           >  2:2836638/1‑93 (MQ=255)

                                                                tACAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGc                          >  2:2049569/1‑93 (MQ=255)

                                                                 aCAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATTGCGGGCTGCTGCACATGGCGGCGAGAATATTGACTTCAAGCTTGCg                         >  2:695543/1‑93 (MQ=255)

                                                                  cAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGa                        >  2:2691020/1‑93 (MQ=255)

                                                                  cAGATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTAGCGa                        >  1:2991708/1‑93 (MQ=255)

                                                                    gATTCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAAt                      >  1:3996795/1‑93 (MQ=255)

                                                                     aTTCGATGTTACGACAAATGAT‑TCC‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATc                     <  2:2296168/93‑1 (MQ=255)

                                                                      ttCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCt                    >  1:4918116/1‑93 (MQ=255)

                                                                       tCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTg                   >  1:2513046/1‑93 (MQ=255)

                                                                       tCGATGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGCCGAGAATATTGCCTTCAAGCTGGCGAATCTg                   >  1:4759254/1‑93 (MQ=255)

                                                                           tGTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGt               >  1:5996749/1‑93 (MQ=255)

                                                                            gTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGtt              >  1:4583477/1‑93 (MQ=255)

                                                                            gTTACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGACTTCAAGCTGGCGAATCTTcccgt              >  2:3465017/1‑90 (MQ=255)

                                                                             ttACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGACTTCAAGCTGGCGAATCTGGCGttc             >  2:1286108/1‑93 (MQ=255)

                                                                              tACGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGttct            <  2:5838014/93‑1 (MQ=255)

                                                                               aCGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGttctt           >  2:453003/1‑93 (MQ=255)

                                                                               aCGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGttctt           <  2:2269049/93‑1 (MQ=255)

                                                                                cGACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGttcttc          <  1:4838556/93‑1 (MQ=255)

                                                                                 gACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCg         >  2:5222944/1‑93 (MQ=255)

                                                                                 gACAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCg         <  2:119444/93‑1 (MQ=255)

                                                                                 gACAAATGAT‑T‑TACCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCg         >  2:793141/1‑93 (MQ=255)

                                                                                   cAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGGCGTTCTTCGGt       >  1:4672639/1‑93 (MQ=255)

                                                                                   cAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGt       >  1:5442338/1‑93 (MQ=255)

                                                                                   cAAATGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGt       >  1:3165911/1‑93 (MQ=255)

                                                                                      aTGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGat    >  2:4125335/1‑93 (MQ=255)

                                                                                       tGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGTCGTTCTTCGGTGata   >  2:6081144/1‑93 (MQ=255)

                                                                                       tGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGata   <  1:934959/93‑1 (MQ=255)

                                                                                       tGAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGata   <  1:63512/93‑1 (MQ=255)

                                                                                        gAT‑T‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGatat  >  1:6285105/1‑93 (MQ=255)

ATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T‑T‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATAT  >  NC_000913/1562839‑1563018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑T‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTC  >  NC_000913/1562846‑1563029

CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑T‑CCCCCcat                                                                                            >  SRR4302533.3043565/1‑91 (MQ=60)

 CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATT                                                                                            >  SRR4302533.3571307/1‑94 (MQ=60)

 CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATT                                                                                            >  SRR4302533.3000355/1‑94 (MQ=60)

  AGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTT                                                                                           >  SRR4302533.2606175/1‑94 (MQ=60)

   GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTC                                                                                          >  SRR4302533.2986875/1‑94 (MQ=60)

   GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTC                                                                                          >  SRR4302533.4445543/1‑94 (MQ=60)

     GTATCGGCATCCGGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCAT                                                                                        <  SRR4302533.4259257/94‑1 (MQ=60)

     GTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCAT                                                                                        >  SRR4302533.4171660/1‑94 (MQ=60)

      TATCGCCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATG                                                                                       <  SRR4302533.1139481/94‑1 (MQ=60)

       ATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGA                                                                                      <  SRR4302533.5411511/94‑1 (MQ=60)

       ATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGA                                                                                      <  SRR4302533.6301486/94‑1 (MQ=60)

         CGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAA                                                                                    >  SRR4302533.4224816/1‑94 (MQ=60)

          GGCATCCCGTTGCGCAGTGCGAACAGCGAGGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAAT                                                                                   <  SRR4302533.6098468/94‑1 (MQ=60)

          GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAAT                                                                                   <  SRR4302533.5970735/94‑1 (MQ=60)

          CGCATCCCGTTGCGCAGTGCGAACCGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAAT                                                                                   <  SRR4302533.1391130/94‑1 (MQ=60)

           GCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATG                                                                                  <  SRR4302533.3302839/94‑1 (MQ=60)

            CATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGG                                                                                 <  SRR4302533.4338571/94‑1 (MQ=60)

             ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGC                                                                                >  SRR4302533.4898766/1‑94 (MQ=60)

             ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGC                                                                                >  SRR4302533.753875/1‑94 (MQ=60)

             ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGC                                                                                <  SRR4302533.5758779/94‑1 (MQ=60)

             ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGC                                                                                >  SRR4302533.75586/1‑94 (MQ=60)

              TCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCG                                                                               <  SRR4302533.367013/94‑1 (MQ=60)

               CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGG                                                                              >  SRR4302533.1658325/1‑94 (MQ=60)

               CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGG                                                                              >  SRR4302533.5104309/1‑94 (MQ=60)

               CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGG                                                                              >  SRR4302533.4217192/1‑94 (MQ=60)

                CCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGG                                                                             <  SRR4302533.5963228/94‑1 (MQ=60)

                CCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGG                                                                             <  SRR4302533.302365/94‑1 (MQ=60)

                  GTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCT                                                                           >  SRR4302533.1832712/1‑94 (MQ=60)

                     GCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCT                                                                        <  SRR4302533.5425238/94‑1 (MQ=60)

                       GCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGC                                                                      >  SRR4302533.4837692/1‑94 (MQ=60)

                        CAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCC                                                                     >  SRR4302533.377809/1‑94 (MQ=60)

                        CAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCA                                                                     >  SRR4302533.3848033/1‑94 (MQ=60)

                          GTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACA                                                                   >  SRR4302533.426223/1‑94 (MQ=60)

                          GTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACA                                                                   >  SRR4302533.5520950/1‑94 (MQ=60)

                           TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT                                                                  >  SRR4302533.6133157/1‑94 (MQ=60)

                           TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT                                                                  >  SRR4302533.4616017/1‑94 (MQ=60)

                           TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT                                                                  <  SRR4302533.1101879/94‑1 (MQ=60)

                            GCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATG                                                                 <  SRR4302533.6109095/94‑1 (MQ=60)

                             CGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGG                                                                <  SRR4302533.2996618/94‑1 (MQ=60)

                              GAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATAGC                                                               >  SRR4302533.4528280/1‑94 (MQ=60)

                               AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCG                                                              <  SRR4302533.4777481/94‑1 (MQ=60)

                               AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCG                                                              <  SRR4302533.3178318/94‑1 (MQ=60)

                                ACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGG                                                             >  SRR4302533.3764697/1‑94 (MQ=60)

                                ACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGG                                                             >  SRR4302533.3500978/1‑94 (MQ=60)

                                 CAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGC                                                            <  SRR4302533.6280987/94‑1 (MQ=60)

                                  AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG                                                           >  SRR4302533.1595348/1‑94 (MQ=60)

                                  AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG                                                           <  SRR4302533.1752636/94‑1 (MQ=60)

                                  AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG                                                           >  SRR4302533.2178033/1‑94 (MQ=60)

                                    CGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG                                                         <  SRR4302533.3901009/94‑1 (MQ=60)

                                     GAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGA                                                        >  SRR4302533.3372812/1‑94 (MQ=60)

                                       AGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAAT                                                      <  SRR4302533.2173241/94‑1 (MQ=60)

                                         CATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAT                                                    <  SRR4302533.1021613/94‑1 (MQ=60)

                                         CATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAT                                                    <  SRR4302533.3996551/94‑1 (MQ=60)

                                         CATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAT                                                    >  SRR4302533.197750/1‑94 (MQ=60)

                                          ATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATT                                                   >  SRR4302533.404300/1‑94 (MQ=60)

                                           TGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTG                                                  <  SRR4302533.4398183/94‑1 (MQ=60)

                                            GCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGC                                                 <  SRR4302533.4959785/94‑1 (MQ=60)

                                             CGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCC                                                >  SRR4302533.4747293/1‑94 (MQ=60)

                                             CGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCC                                                <  SRR4302533.5855414/94‑1 (MQ=60)

                                              GATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCT                                               <  SRR4302533.1304752/94‑1 (MQ=60)

                                               ATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTT                                              <  SRR4302533.1394142/94‑1 (MQ=60)

                                               ATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTT                                              >  SRR4302533.1618466/1‑94 (MQ=60)

                                                TTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTC                                             <  SRR4302533.5921461/94‑1 (MQ=60)

                                                 TCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCA                                            <  SRR4302533.210377/94‑1 (MQ=60)

                                                 TCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCA                                            <  SRR4302533.1952136/94‑1 (MQ=60)

                                                 TCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCA                                            <  SRR4302533.1543947/94‑1 (MQ=60)

                                                  CGTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAA                                           <  SRR4302533.2606993/94‑1 (MQ=60)

                                                   GTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAG                                          <  SRR4302533.1163405/94‑1 (MQ=60)

                                                   CTTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAG                                          <  SRR4302533.1189725/94‑1 (MQ=60)

                                                    TTGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGC                                         >  SRR4302533.322189/1‑94 (MQ=60)

                                                     TGAGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCT                                        <  SRR4302533.4747394/94‑1 (MQ=60)

                                                       AGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGGAAATTGGCTTCAAGCTTG                                      >  SRR4302533.5029051/1‑94 (MQ=60)

                                                       AGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGG                                      <  SRR4302533.3807917/94‑1 (MQ=60)

                                                       AGTACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGG                                      <  SRR4302533.4973207/94‑1 (MQ=60)

                                                         TACAGATTCGATGTTACGACAAATGATTCC‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCG                                    <  SRR4302533.63408/94‑1 (MQ=60)

                                                          ACAGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTAGCGA                                   >  SRR4302533.3010003/1‑94 (MQ=60)

                                                            AGATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAAT                                 >  SRR4302533.4021133/1‑94 (MQ=60)

                                                             GATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATC                                >  SRR4302533.1423444/1‑94 (MQ=60)

                                                              ATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCT                               >  SRR4302533.4947910/1‑94 (MQ=60)

                                                              ATTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTCCCTTCAAGCTGGCGAATCT                               <  SRR4302533.1134170/94‑1 (MQ=60)

                                                               TTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTG                              >  SRR4302533.2528531/1‑94 (MQ=60)

                                                               TTCGATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGCCGAGAATATTGCCTTCAAGCTGGCGAATCTG                              >  SRR4302533.4788125/1‑94 (MQ=60)

                                                                  GATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCG                           <  SRR4302533.6008404/94‑1 (MQ=60)

                                                                  GATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCG                           <  SRR4302533.1100536/94‑1 (MQ=60)

                                                                  GATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCG                           >  SRR4302533.1235198/1‑94 (MQ=60)

                                                                   ATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGGCGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGT                          >  SRR4302533.2678903/1‑94 (MQ=60)

                                                                   ATGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGT                          >  SRR4302533.6033070/1‑94 (MQ=60)

                                                                    TGTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTT                         >  SRR4302533.4611246/1‑94 (MQ=60)

                                                                     GTTACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTC                        >  SRR4302533.2470404/1‑94 (MQ=60)

                                                                        ACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTC                     >  SRR4302533.5285005/1‑94 (MQ=60)

                                                                        ACGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTC                     >  SRR4302533.2595933/1‑94 (MQ=60)

                                                                         CGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCG                    >  SRR4302533.177126/1‑94 (MQ=60)

                                                                         CGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCG                    <  SRR4302533.4867883/94‑1 (MQ=60)

                                                                         CGACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCG                    >  SRR4302533.720536/1‑94 (MQ=60)

                                                                           ACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGGCGTTCTTCGGT                  >  SRR4302533.4700954/1‑94 (MQ=60)

                                                                           ACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGT                  >  SRR4302533.5475122/1‑94 (MQ=60)

                                                                           ACAAATGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGT                  >  SRR4302533.3185393/1‑94 (MQ=60)

                                                                                TGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATAT             <  SRR4302533.940448/94‑1 (MQ=60)

                                                                                TGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATAT             >  SRR4302533.6322608/1‑94 (MQ=60)

                                                                                TGATT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATAT             <  SRR4302533.63927/94‑1 (MQ=60)

                                                                                   TT‑TCCCCCCATTTCATGAAATGGCGGGCTGCTACACATGGCGGTGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCG          <  SRR4302533.5791346/94‑1 (MQ=60)

                                                                                   TT‑C‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGA         >  SRR4302533.5805088/1‑94 (MQ=60)

                                                                                    ttccCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCG          <  SRR4302533.540193/90‑1 (MQ=60)

                                                                                      C‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGA       >  SRR4302533.699361/1‑94 (MQ=60)

                                                                                      C‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGA       >  SRR4302533.5510805/1‑94 (MQ=60)

                                                                                      C‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGA       <  SRR4302533.3778531/94‑1 (MQ=60)

                                                                                        CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAA      <  SRR4302533.355433/94‑1 (MQ=60)

                                                                                           CCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGT   <  SRR4302533.1112323/94‑1 (MQ=60)

                                                                                            CATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTC  <  SRR4302533.5895300/94‑1 (MQ=60)

                                                                                            CATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTC  >  SRR4302533.5211162/1‑94 (MQ=60)

CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT‑T‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATATTGCCTTCAAGCTGGCGAATCTGCCGTTCTTCGGTGATATCCGAGAAAGTC  >  NC_000913/1562846‑1563029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`