Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,693,549 A→G W183R (TGG→CGG)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,693,5490AG100.0% 691.9 / NA 217W183R (TGG→CGG) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (88/129);  total (88/129)

Only 100 of 226 total aligned reads displayed.

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638
                                                                                          |                                                                                         
tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                         <  1:2016458/93‑1 (MQ=255)
  aCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCgggcg                                                                                       >  1:2295936/1‑91 (MQ=255)
    ctCACCAATGGCCACCACCAGCCAGTCAACAGACGCGTGGTTACAGGCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     <  2:5364379/92‑1 (MQ=255)
     tCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTc                                                                                    <  2:2661406/93‑1 (MQ=255)
       aCCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCgg                                                                                  >  2:5071505/1‑93 (MQ=255)
       aCCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCgg                                                                                  >  2:4706622/1‑93 (MQ=255)
          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                               >  2:4332025/1‑93 (MQ=255)
            tGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtgg                                                                             <  1:2615619/93‑1 (MQ=255)
             ggCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggt                                                                            <  1:1921018/93‑1 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         >  2:5561493/1‑93 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         >  2:1772488/1‑93 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         <  2:4583498/93‑1 (MQ=255)
                 cccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                        <  2:3832542/92‑1 (MQ=255)
                 accaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                        <  1:3103901/93‑1 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       >  1:3044322/1‑93 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  1:1248873/93‑1 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  2:5898124/93‑1 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGCGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       >  2:4475560/1‑93 (MQ=255)
                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                  <  1:3168655/93‑1 (MQ=255)
                       cgCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                  <  1:4718706/92‑1 (MQ=255)
                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                 <  2:3167970/93‑1 (MQ=255)
                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                 <  1:1125247/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTTCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              >  2:6153704/1‑93 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCCCCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  1:2129632/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  2:2165528/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              >  1:3984551/1‑93 (MQ=255)
                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                             <  1:699902/93‑1 (MQ=255)
                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGGAGAGCATTACGCTg                                                            >  1:4590212/1‑93 (MQ=255)
                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  2:1567222/93‑1 (MQ=255)
                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           >  2:1230112/1‑93 (MQ=255)
                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  2:2725683/93‑1 (MQ=255)
                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  1:806806/93‑1 (MQ=255)
                                 cAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGAt                                                        >  2:771556/1‑93 (MQ=255)
                                  aGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATg                                                       <  1:5031445/93‑1 (MQ=255)
                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                      <  1:5847215/93‑1 (MQ=255)
                                   gACGCGTGGTTACAATCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                      <  1:3790700/93‑1 (MQ=255)
                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                     <  1:4424252/93‑1 (MQ=255)
                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                     <  2:2375892/93‑1 (MQ=255)
                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                     >  2:6304225/1‑93 (MQ=255)
                                     cgcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGAt                                                    >  2:4605714/1‑93 (MQ=255)
                                     cgcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGAt                                                    <  1:1528780/93‑1 (MQ=255)
                                      gcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATc                                                   <  2:1763858/93‑1 (MQ=255)
                                       cgTTGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATcc                                                  <  2:455260/93‑1 (MQ=255)
                                       cgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATcc                                                  <  1:2971725/93‑1 (MQ=255)
                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                 <  2:4898375/93‑1 (MQ=255)
                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                 >  2:5664547/1‑93 (MQ=255)
                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                 <  2:3368162/93‑1 (MQ=255)
                                             tACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCAt                                            >  2:5170588/1‑93 (MQ=255)
                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                           <  1:4230823/93‑1 (MQ=255)
                                               cAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAg                                          >  2:2030768/1‑93 (MQ=255)
                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                         <  1:5394104/93‑1 (MQ=255)
                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGtt                                        >  2:2257284/1‑93 (MQ=255)
                                                  tCTTGCGCGACATGCGTCACCACGGGGATATCGTCCACCCGGGTGTTCGGCGTGGGGGAGAGCATTACGCCGCGATGGATCCCCGCATAGATa                                       >  1:5307757/1‑93 (MQ=255)
                                                    ttGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTaaa                                     <  1:5581239/93‑1 (MQ=255)
                                                    ttGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTaaa                                     <  1:4780399/93‑1 (MQ=255)
                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGa                                   <  1:3961131/93‑1 (MQ=255)
                                                        gcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGaaa                                 >  2:4244562/1‑93 (MQ=255)
                                                        gcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGaaa                                 <  1:146866/93‑1 (MQ=255)
                                                        gcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGaaa                                 <  2:4401383/93‑1 (MQ=255)
                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               >  2:4785854/1‑93 (MQ=255)
                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               >  1:28875/1‑93 (MQ=255)
                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               <  1:2052379/93‑1 (MQ=255)
                                                           aCATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              <  2:1340132/93‑1 (MQ=255)
                                                           aCATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              >  2:1375200/1‑93 (MQ=255)
                                                           aCATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              >  2:5932382/1‑93 (MQ=255)
                                                           aCATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              >  1:3680092/1‑93 (MQ=255)
                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                             >  1:1931063/1‑93 (MQ=255)
                                                             aTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            <  1:669266/93‑1 (MQ=255)
                                                             aTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            <  1:4454487/93‑1 (MQ=255)
                                                             aTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            <  1:1007015/93‑1 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                           <  2:6053542/93‑1 (MQ=255)
                                                               gCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGa                          <  2:4794242/93‑1 (MQ=255)
                                                               gCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGa                          <  1:279520/93‑1 (MQ=255)
                                                                cGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGaa                         >  2:3481673/1‑93 (MQ=255)
                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                        <  2:2543243/93‑1 (MQ=255)
                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                        >  2:3764225/1‑93 (MQ=255)
                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGt                       <  1:166031/93‑1 (MQ=255)
                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGt                       <  1:5621176/93‑1 (MQ=255)
                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGTGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     <  1:5451046/93‑1 (MQ=255)
                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     >  1:6173450/1‑93 (MQ=255)
                                                                     ccacGGTGATATCGTCCGCCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                    <  2:5799985/93‑1 (MQ=255)
                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                    >  1:105895/1‑93 (MQ=255)
                                                                      cacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGa                   <  2:3073391/93‑1 (MQ=255)
                                                                      cacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGa                   <  1:3189133/93‑1 (MQ=255)
                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  <  1:5270049/93‑1 (MQ=255)
                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  >  1:219587/1‑93 (MQ=255)
                                                                        cGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACt                 <  1:2592733/93‑1 (MQ=255)
                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  1:3768288/93‑1 (MQ=255)
                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt             <  1:389399/93‑1 (MQ=255)
                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt             <  2:4436410/93‑1 (MQ=255)
                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           <  2:1127829/93‑1 (MQ=255)
                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  1:347465/93‑1 (MQ=255)
                                                                                  gTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt       <  1:4543605/93‑1 (MQ=255)
                                                                                  gTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt       <  1:1730964/93‑1 (MQ=255)
                                                                                  gTCCACCCGGGTGTTCGGCGTAGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt       <  2:1633512/93‑1 (MQ=255)
                                                                                   tCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTg      >  2:1740445/1‑93 (MQ=255)
                                                                                    tcACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGc     <  1:5078385/92‑1 (MQ=255)
                                                                                    ccACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGc     >  1:1503430/1‑93 (MQ=255)
                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc    <  1:4122914/93‑1 (MQ=255)
                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc    >  1:1548205/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  >  NC_000913/1693457‑1693642
                                                                                            |                                                                                             
GCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGG                                                                                              <  SRR4302533.2864447/94‑1 (MQ=60)
GCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGG                                                                                              >  SRR4302533.3530056/1‑94 (MQ=60)
  TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGT                                                                                            <  SRR4302533.2028882/94‑1 (MQ=60)
   GACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTG                                                                                           >  SRR4302533.6322923/1‑94 (MQ=60)
   GACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTG                                                                                           <  SRR4302533.1512211/94‑1 (MQ=60)
    ACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGT                                                                                          <  SRR4302533.1584943/94‑1 (MQ=60)
     CATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTT                                                                                         >  SRR4302533.4643502/1‑94 (MQ=60)
      ATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTC                                                                                        >  SRR4302533.3146300/1‑94 (MQ=60)
         CCCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGTGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGC                                                                                     <  SRR4302533.4476029/94‑1 (MQ=60)
         ACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGC                                                                                     >  SRR4302533.1527288/1‑94 (MQ=60)
         ACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGC                                                                                     >  SRR4302533.942541/1‑94 (MQ=60)
          CCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCG                                                                                    >  SRR4302533.4263771/1‑94 (MQ=60)
          CCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCG                                                                                    >  SRR4302533.972720/1‑94 (MQ=60)
           CATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGT                                                                                   >  SRR4302533.3272750/1‑94 (MQ=60)
           CATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGCGTTCGGCGT                                                                                   >  SRR4302533.1389062/1‑94 (MQ=60)
             TTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGG                                                                                 <  SRR4302533.1069330/94‑1 (MQ=60)
              TGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGT                                                                                >  SRR4302533.30138/1‑94 (MQ=60)
              TGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGT                                                                                <  SRR4302533.2631673/94‑1 (MQ=60)
               GGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTG                                                                               <  SRR4302533.1132934/94‑1 (MQ=60)
               GGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTG                                                                               <  SRR4302533.1932745/94‑1 (MQ=60)
                 CCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTA                                                                             <  SRR4302533.2693839/94‑1 (MQ=60)
                  CACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGTGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAG                                                                            >  SRR4302533.3548875/1‑94 (MQ=60)
                  CACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAG                                                                            <  SRR4302533.4797018/94‑1 (MQ=60)
                   ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGA                                                                           <  SRR4302533.3122984/94‑1 (MQ=60)
                   ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGA                                                                           >  SRR4302533.3062956/1‑94 (MQ=60)
                    CCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAG                                                                          <  SRR4302533.1256333/94‑1 (MQ=60)
                    CCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAG                                                                          >  SRR4302533.2621305/1‑94 (MQ=60)
                    CCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAG                                                                          <  SRR4302533.3971511/94‑1 (MQ=60)
                     CACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGC                                                                         <  SRR4302533.2916725/94‑1 (MQ=60)
                     CACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGC                                                                         <  SRR4302533.4080293/94‑1 (MQ=60)
                      ACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCA                                                                        >  SRR4302533.422310/1‑94 (MQ=60)
                      ACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCA                                                                        >  SRR4302533.215847/1‑94 (MQ=60)
                        CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302533.5376034/1‑94 (MQ=60)
                         TGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTA                                                                     >  SRR4302533.3795925/1‑94 (MQ=60)
                         TGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTA                                                                     <  SRR4302533.3188155/94‑1 (MQ=60)
                         CGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTA                                                                     <  SRR4302533.4747320/94‑1 (MQ=60)
                          GCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAC                                                                    <  SRR4302533.1131926/94‑1 (MQ=60)
                           CCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACG                                                                   <  SRR4302533.5758070/94‑1 (MQ=60)
                            CAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGC                                                                  >  SRR4302533.4008813/1‑94 (MQ=60)
                             AGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCCCCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCT                                                                 <  SRR4302533.2142766/94‑1 (MQ=60)
                             AGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCT                                                                 <  SRR4302533.1631533/94‑1 (MQ=60)
                              GTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTG                                                                <  SRR4302533.704045/94‑1 (MQ=60)
                              GTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGGAGAGCATTACGCTG                                                                >  SRR4302533.4618016/1‑94 (MQ=60)
                               TCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGC                                                               >  SRR4302533.2266936/1‑94 (MQ=60)
                               TCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGC                                                               <  SRR4302533.4721752/94‑1 (MQ=60)
                               TCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGC                                                               <  SRR4302533.1757512/94‑1 (MQ=60)
                                CAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCG                                                              <  SRR4302533.811547/94‑1 (MQ=60)
                                    AGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGG                                                          <  SRR4302533.5061910/94‑1 (MQ=60)
                                    AGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGG                                                          >  SRR4302533.2019672/1‑94 (MQ=60)
                                     GACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGA                                                         <  SRR4302533.2234913/94‑1 (MQ=60)
                                     GACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGA                                                         <  SRR4302533.5938338/94‑1 (MQ=60)
                                     GACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGA                                                         <  SRR4302533.5882564/94‑1 (MQ=60)
                                     GACGCGTGGTTACAATCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGA                                                         <  SRR4302533.3813911/94‑1 (MQ=60)
                                      ACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGAT                                                        <  SRR4302533.4450996/94‑1 (MQ=60)
                                       CGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATC                                                       <  SRR4302533.1537976/94‑1 (MQ=60)
                                       CGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATC                                                       >  SRR4302533.1934552/1‑94 (MQ=60)
                                         CGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCC                                                     <  SRR4302533.2989908/94‑1 (MQ=60)
                                          GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    <  SRR4302533.4846598/94‑1 (MQ=60)
                                          GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    >  SRR4302533.4439924/1‑94 (MQ=60)
                                          GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    >  SRR4302533.3180584/1‑94 (MQ=60)
                                           TGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGG                                                   >  SRR4302533.74846/1‑94 (MQ=60)
                                             GTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCA                                                 >  SRR4302533.3989179/1‑94 (MQ=60)
                                              TTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCAT                                                <  SRR4302533.3938906/94‑1 (MQ=60)
                                              TTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCAT                                                <  SRR4302533.20485/94‑1 (MQ=60)
                                                ACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAG                                              <  SRR4302533.4256396/94‑1 (MQ=60)
                                                ACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAG                                              <  SRR4302533.2820903/94‑1 (MQ=60)
                                                  AGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTT                                            <  SRR4302533.5426516/94‑1 (MQ=60)
                                                  AGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTT                                            >  SRR4302533.2107522/1‑94 (MQ=60)
                                                   GTCTTGCGCGACATGCGTCACCACGGGGATATCGTCCACCCGGGTGTTCGGCGTGGGGGAGAGCATTACGCCGCGATGGATCCCCGCATAGATA                                           >  SRR4302533.5339569/1‑94 (MQ=60)
                                                      TTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAG                                        <  SRR4302533.4809360/94‑1 (MQ=60)
                                                      TTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAG                                        <  SRR4302533.5062138/94‑1 (MQ=60)
                                                      TTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAG                                        <  SRR4302533.699293/94‑1 (MQ=60)
                                                      TTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAG                                        <  SRR4302533.5614966/94‑1 (MQ=60)
                                                      TTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAG                                        <  SRR4302533.5643600/94‑1 (MQ=60)
                                                        GCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAA                                      <  SRR4302533.3985264/94‑1 (MQ=60)
                                                        GCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAA                                      <  SRR4302533.5181540/94‑1 (MQ=60)
                                                          GCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAT                                    <  SRR4302533.147805/94‑1 (MQ=60)
                                                           CGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATC                                   >  SRR4302533.29070/1‑94 (MQ=60)
                                                           CGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATC                                   >  SRR4302533.3562324/1‑94 (MQ=60)
                                                            GACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCA                                  >  SRR4302533.3702708/1‑94 (MQ=60)
                                                            GACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCA                                  <  SRR4302533.2065043/94‑1 (MQ=60)
                                                             ACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAT                                 >  SRR4302533.1942842/1‑94 (MQ=60)
                                                             ACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAT                                 >  SRR4302533.5324466/1‑94 (MQ=60)
                                                              CATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATG                                <  SRR4302533.4511197/94‑1 (MQ=60)
                                                               ATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGG                               <  SRR4302533.673219/94‑1 (MQ=60)
                                                               ATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGG                               <  SRR4302533.1012907/94‑1 (MQ=60)
                                                               ATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGG                               <  SRR4302533.4481420/94‑1 (MQ=60)
                                                                 GCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAA                             <  SRR4302533.106374/94‑1 (MQ=60)
                                                                 GCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAA                             <  SRR4302533.281226/94‑1 (MQ=60)
                                                                 GCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAA                             <  SRR4302533.3873213/94‑1 (MQ=60)
                                                                   GTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGT                           <  SRR4302533.5435820/94‑1 (MQ=60)
                                                                    TCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTA                          <  SRR4302533.167084/94‑1 (MQ=60)
                                                                    TCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTA                          <  SRR4302533.5655152/94‑1 (MQ=60)
                                                                     CACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAA                         >  SRR4302533.6210547/1‑94 (MQ=60)
                                                                     CACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAA                         >  SRR4302533.3726556/1‑94 (MQ=60)
                                                                     CACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAA                         >  SRR4302533.5540531/1‑94 (MQ=60)
                                                                      ACCACGGTGATATCGTCCACCCGGGTGTTCGGTGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAG                        <  SRR4302533.5483885/94‑1 (MQ=60)
                                                                      ACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAG                        >  SRR4302533.106554/1‑94 (MQ=60)
                                                                      ACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAG                        <  SRR4302533.1705589/94‑1 (MQ=60)
                                                                        CACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAC                      >  SRR4302533.220961/1‑94 (MQ=60)
                                                                        CACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAC                      <  SRR4302533.3208749/94‑1 (MQ=60)
                                                                        CACGGTGATATCGTCCACCCGGGTGTTCGGCGCGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAC                      >  SRR4302533.2415856/1‑94 (MQ=60)
                                                                         ACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACT                     <  SRR4302533.5301655/94‑1 (MQ=60)
                                                                         ACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACT                     <  SRR4302533.1338160/94‑1 (MQ=60)
                                                                          CGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTG                    <  SRR4302533.2608666/94‑1 (MQ=60)
                                                                           GGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGC                   <  SRR4302533.4607/94‑1 (MQ=60)
                                                                             TGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTT                 <  SRR4302533.3791395/94‑1 (MQ=60)
                                                                              GATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTT                >  SRR4302533.2866765/1‑94 (MQ=60)
                                                                              GATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTT                <  SRR4302533.391751/94‑1 (MQ=60)
                                                                              GATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTT                >  SRR4302533.5515397/1‑94 (MQ=60)
                                                                               ATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTT               <  SRR4302533.5170246/94‑1 (MQ=60)
                                                                                TATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTT              <  SRR4302533.1983239/94‑1 (MQ=60)
                                                                                TATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTT              <  SRR4302533.3203570/94‑1 (MQ=60)
                                                                                   CGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTT           <  SRR4302533.349585/94‑1 (MQ=60)
                                                                                    GTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTG          <  SRR4302533.1741459/94‑1 (MQ=60)
                                                                                    GTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTG          <  SRR4302533.4571119/94‑1 (MQ=60)
                                                                                     TCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGC         <  SRR4302533.1556239/94‑1 (MQ=60)
                                                                                     TCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGC         >  SRR4302533.1512495/1‑94 (MQ=60)
                                                                                      TCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCC        <  SRR4302533.5109078/94‑1 (MQ=60)
                                                                                      CCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCC        <  SRR4302533.6006493/94‑1 (MQ=60)
                                                                                      CCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCC        >  SRR4302533.1557535/1‑94 (MQ=60)
                                                                                       CACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCG       <  SRR4302533.4147948/94‑1 (MQ=60)
                                                                                        ACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT      >  SRR4302533.3104596/1‑94 (MQ=60)
                                                                                          CCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTT    <  SRR4302533.1398132/94‑1 (MQ=60)
                                                                                          CCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTT    <  SRR4302533.3967833/94‑1 (MQ=60)
                                                                                           CGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT   >  SRR4302533.3157950/1‑94 (MQ=60)
                                                                                            GGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  >  SRR4302533.1103607/1‑94 (MQ=60)
                                                                                            GGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  <  SRR4302533.991080/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  >  NC_000913/1693457‑1693642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑