Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,693,549 A→G W183R (TGG→CGG)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,693,5490AG99.5% 688.2 / ‑5.7 218W183R (TGG→CGG) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (111/106);  total (111/107)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 231 total aligned reads displayed.

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTT  >  NC_000913/1693459‑1693639
                                                                                          |                                                                                          
tGACATCACCATTGGCCACCGCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          <  2:5146823/93‑1 (MQ=255)
tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          <  1:9083450/93‑1 (MQ=255)
tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          >  1:7309011/1‑93 (MQ=255)
 gACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                         <  1:366656/93‑1 (MQ=255)
 gACATCACCATTGGCCAACACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCgggg                                                                                         >  2:1059426/1‑92 (MQ=255)
  aCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtg                                                                                        <  1:8832514/93‑1 (MQ=255)
     tCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTc                                                                                     >  1:2990649/1‑93 (MQ=255)
      cACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCg                                                                                    >  1:8146783/1‑93 (MQ=255)
         cATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCg                                                                                 >  2:9396596/1‑93 (MQ=255)
          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                                >  1:7196365/1‑93 (MQ=255)
           ttGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtg                                                                               <  1:4993061/93‑1 (MQ=255)
             ggCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggt                                                                             <  1:9604800/93‑1 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          >  1:172470/1‑93 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          <  1:6547623/93‑1 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          >  2:6343626/1‑93 (MQ=255)
                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGGTTTCGGCGTGGTGTa                                                                          >  1:5915450/1‑93 (MQ=255)
                 cccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                         <  2:8264503/92‑1 (MQ=255)
                 accaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                         >  2:7566574/1‑93 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        >  1:1613055/1‑93 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        >  2:4221949/1‑93 (MQ=255)
                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        <  1:4917854/93‑1 (MQ=255)
                   caccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTagag                                                                       <  2:2221809/93‑1 (MQ=255)
                   caccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTagac                                                                       >  1:9004496/1‑92 (MQ=255)
                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:8526593/1‑93 (MQ=255)
                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:782211/1‑93 (MQ=255)
                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:4674564/1‑93 (MQ=255)
                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:1247058/1‑93 (MQ=255)
                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                  <  1:3113167/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  2:4790573/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               >  1:169362/1‑93 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  1:2740528/93‑1 (MQ=255)
                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  2:5704634/93‑1 (MQ=255)
                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                              >  1:6760169/1‑93 (MQ=255)
                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                              >  2:2248772/1‑93 (MQ=255)
                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                             >  1:5141715/1‑93 (MQ=255)
                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                             >  2:1703656/1‑93 (MQ=255)
                               aaCAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCg                                                           >  1:3386750/1‑93 (MQ=255)
                                 cAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGAt                                                         <  1:9535032/93‑1 (MQ=255)
                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                       <  1:1082820/93‑1 (MQ=255)
                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                       >  1:10008739/1‑93 (MQ=255)
                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                      <  1:7074039/93‑1 (MQ=255)
                                       cgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGACATCGTCCACCCGGGTGTTCGGCGTGGGGTAGAGCATTACGCTGCGATGGATcc                                                   >  2:1968250/1‑93 (MQ=255)
                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                  <  1:9346165/93‑1 (MQ=255)
                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                  <  2:1845391/93‑1 (MQ=255)
                                         tGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCg                                                 >  1:4122494/1‑93 (MQ=255)
                                         tGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGCGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCg                                                 >  2:4139575/1‑93 (MQ=255)
                                          ggTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCggg                                                >  1:7623825/1‑93 (MQ=255)
                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGc                                               <  2:8533304/93‑1 (MQ=255)
                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGCTCCCGGCATa                                            >  2:5266591/1‑93 (MQ=255)
                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                            <  2:1222892/93‑1 (MQ=255)
                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                            >  2:2864998/1‑93 (MQ=255)
                                               cAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAg                                           <  1:5941496/93‑1 (MQ=255)
                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                          >  2:1362143/1‑93 (MQ=255)
                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGtt                                         >  2:8302456/1‑93 (MQ=255)
                                                   cTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTaa                                       <  1:8767548/93‑1 (MQ=255)
                                                      tcgcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGa                                    <  1:1568945/92‑1 (MQ=255)
                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTTCGCTGCGATGGATCCCGGCATAGTTAAAGa                                    <  2:2316263/93‑1 (MQ=255)
                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTGAAAGa                                    <  2:5727754/93‑1 (MQ=255)
                                                       cgcgACATGCGTCACCACGCTGATACCGACCGCCCGGGTGCCCGGCGTGCTGTAGAGCCTTACGCTGCGATGGATTCCGGCAAAGTTAAAGaa                                   <  2:6994101/93‑1 (MQ=255)
                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                 <  2:704168/93‑1 (MQ=255)
                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                 <  1:9200129/93‑1 (MQ=255)
                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                                >  1:2237669/1‑93 (MQ=255)
                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              <  2:7295754/93‑1 (MQ=255)
                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              >  2:5077795/1‑93 (MQ=255)
                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              <  2:4490139/93‑1 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            >  2:2697146/1‑93 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  2:6267128/93‑1 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  1:9974877/93‑1 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  1:372760/93‑1 (MQ=255)
                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  2:8836875/93‑1 (MQ=255)
                                                                cGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGaa                          <  2:9303573/93‑1 (MQ=255)
                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                         <  1:9694884/93‑1 (MQ=255)
                                                                 gTCACCACGGTGATATCGTCCACCCGGCTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                         <  2:6876290/93‑1 (MQ=255)
                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                          <  2:5222933/59‑1 (MQ=255)
                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                          >  1:5222934/1‑59 (MQ=255)
                                                                  tCACCACGGTGATAACGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGt                        >  2:7167144/1‑93 (MQ=255)
                                                                   caccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTa                       >  2:7958147/1‑93 (MQ=255)
                                                                   caccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTa                       <  2:3115951/93‑1 (MQ=255)
                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                      <  2:4042925/93‑1 (MQ=255)
                                                                    accacGGTGATATCGTCCACACGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                      >  2:6370488/1‑93 (MQ=255)
                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                     <  2:1300005/93‑1 (MQ=255)
                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                     >  2:8194831/1‑93 (MQ=255)
                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                   <  1:4252795/93‑1 (MQ=255)
                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc                >  1:191697/1‑93 (MQ=255)
                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt               <  1:618933/93‑1 (MQ=255)
                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt               <  2:1480522/93‑1 (MQ=255)
                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:1419993/1‑93 (MQ=255)
                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:379346/1‑93 (MQ=255)
                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:316693/1‑93 (MQ=255)
                                                                             atatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttt             <  2:6002700/93‑1 (MQ=255)
                                                                             atatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttt             <  2:1417736/93‑1 (MQ=255)
                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttttt          >  1:6027583/1‑92 (MQ=255)
                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTc          >  2:4529176/1‑93 (MQ=255)
                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt         <  2:4513240/93‑1 (MQ=255)
                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt         <  2:8688218/93‑1 (MQ=255)
                                                                                   tCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTg       >  2:3814912/1‑93 (MQ=255)
                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc     <  2:4760044/93‑1 (MQ=255)
                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc     >  2:9081299/1‑93 (MQ=255)
                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGt   <  1:15013/93‑1 (MQ=255)
                                                                                        ccAGGTGTTCGGCGTGGTGTAGAGCAGTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGtt  <  1:2794574/93‑1 (MQ=255)
                                                                                          |                                                                                          
TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTT  >  NC_000913/1693459‑1693639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  >  NC_000913/1693458‑1693642
                                                                                           |                                                                                             
CTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGG                                                                                             >  SRR4302540.7390608/1‑94 (MQ=60)
 TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGT                                                                                            <  SRR4302540.9192584/94‑1 (MQ=60)
  GACATCACCATTGGCCACCCCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTG                                                                                           <  SRR4302540.5248501/94‑1 (MQ=60)
  GACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTG                                                                                           <  SRR4302540.5248805/94‑1 (MQ=60)
  GACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTG                                                                                           <  SRR4302540.370432/94‑1 (MQ=60)
   ACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGT                                                                                          <  SRR4302540.8934666/94‑1 (MQ=60)
   ACATAACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGT                                                                                          <  SRR4302540.1672844/94‑1 (MQ=60)
    CATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTT                                                                                         <  SRR4302540.6372388/94‑1 (MQ=60)
    CATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTT                                                                                         >  SRR4302540.6187812/1‑94 (MQ=60)
    CATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGCGTT                                                                                         <  SRR4302540.4485854/94‑1 (MQ=60)
     ATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTC                                                                                        >  SRR4302540.3023622/1‑94 (MQ=60)
     ATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTC                                                                                        >  SRR4302540.4378744/1‑94 (MQ=60)
     ATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTC                                                                                        <  SRR4302540.5029230/94‑1 (MQ=60)
     ATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTC                                                                                        >  SRR4302540.3023621/1‑94 (MQ=60)
      TCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCAGGGTGATATCGTCCACCCGGGGGTTCG                                                                                       >  SRR4302540.3192478/1‑94 (MQ=60)
      TCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCG                                                                                       >  SRR4302540.8241357/1‑94 (MQ=60)
        ACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGC                                                                                     >  SRR4302540.4283800/1‑94 (MQ=60)
         CCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCG                                                                                    <  SRR4302540.9112078/94‑1 (MQ=60)
          CATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGT                                                                                   >  SRR4302540.7276579/1‑94 (MQ=60)
           ATTGGCCACCACCTGCCAGTCAACAGACGCGGGGTTACAGTCTTTCGCGACATGCGTCACCACGGGGATATCGTCCACCCGGGTGTTCGTCGTG                                                                                  >  SRR4302540.3494749/1‑94 (MQ=60)
            TTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGG                                                                                 >  SRR4302540.7103786/1‑94 (MQ=60)
            TTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGG                                                                                 <  SRR4302540.5047115/94‑1 (MQ=60)
            TTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGG                                                                                 <  SRR4302540.7340698/94‑1 (MQ=60)
              GGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTG                                                                               <  SRR4302540.9718081/94‑1 (MQ=60)
                CCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCTGCGTGGTGCA                                                                             >  SRR4302540.5164060/1‑94 (MQ=60)
                CCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTA                                                                             >  SRR4302540.174190/1‑94 (MQ=60)
                CCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGGTTTCGGCGTGGTGTA                                                                             >  SRR4302540.5980799/1‑94 (MQ=60)
                 CACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAG                                                                            <  SRR4302540.6620253/94‑1 (MQ=60)
                 CACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAG                                                                            >  SRR4302540.1444680/1‑94 (MQ=60)
                  ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGA                                                                           >  SRR4302540.2073668/1‑94 (MQ=60)
                  ACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGA                                                                           >  SRR4302540.1630730/1‑94 (MQ=60)
                   CCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAG                                                                          <  SRR4302540.4970817/94‑1 (MQ=60)
                   CCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAC                                                                          >  SRR4302540.9112525/1‑94 (MQ=60)
                     ACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCA                                                                        >  SRR4302540.9752056/1‑94 (MQ=60)
                      CCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAT                                                                       <  SRR4302540.1512271/94‑1 (MQ=60)
                      CCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAT                                                                       <  SRR4302540.3831118/94‑1 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.1259784/1‑94 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.8918583/1‑94 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.8625664/1‑94 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.7514602/1‑94 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.790539/1‑94 (MQ=60)
                       CTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATT                                                                      >  SRR4302540.4724588/1‑94 (MQ=60)
                        TGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACAACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTA                                                                     >  SRR4302540.9267590/1‑94 (MQ=60)
                         GCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAC                                                                    >  SRR4302540.1178852/1‑94 (MQ=60)
                         GCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAC                                                                    <  SRR4302540.3147214/94‑1 (MQ=60)
                         GCCAGTCAACAGACGCGTGGTGACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTAC                                                                    <  SRR4302540.7836500/94‑1 (MQ=60)
                           CAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGC                                                                  >  SRR4302540.171059/1‑94 (MQ=60)
                            AGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGTATTACGCT                                                                 >  SRR4302540.2802416/1‑94 (MQ=60)
                            AGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCT                                                                 <  SRR4302540.2770117/94‑1 (MQ=60)
                            AGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCT                                                                 >  SRR4302540.6834883/1‑94 (MQ=60)
                             GTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTG                                                                >  SRR4302540.7188951/1‑94 (MQ=60)
                             GTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTG                                                                >  SRR4302540.5197407/1‑94 (MQ=60)
                             GTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTG                                                                >  SRR4302540.8600861/1‑94 (MQ=60)
                               CAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCG                                                              <  SRR4302540.8144657/94‑1 (MQ=60)
                               CAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCG                                                              >  SRR4302540.9988028/1‑94 (MQ=60)
                               CAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCG                                                              >  SRR4302540.3423705/1‑94 (MQ=60)
                                  CAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATG                                                           <  SRR4302540.9647904/94‑1 (MQ=60)
                                   AGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGG                                                          >  SRR4302540.10125292/1‑94 (MQ=60)
                                    GACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGA                                                         <  SRR4302540.1093918/94‑1 (MQ=60)
                                     ACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGAT                                                        <  SRR4302540.7152749/94‑1 (MQ=60)
                                      CGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATC                                                       >  SRR4302540.6881288/1‑94 (MQ=60)
                                        CGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCC                                                     <  SRR4302540.2899081/94‑1 (MQ=60)
                                         GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    >  SRR4302540.4167016/1‑94 (MQ=60)
                                         GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    <  SRR4302540.9457502/94‑1 (MQ=60)
                                         GTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCG                                                    <  SRR4302540.6790601/94‑1 (MQ=60)
                                          TGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCGGG                                                   >  SRR4302540.7709563/1‑94 (MQ=60)
                                              TACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATA                                               <  SRR4302540.9664227/94‑1 (MQ=60)
                                                CAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGT                                             <  SRR4302540.6007175/94‑1 (MQ=60)
                                                 AGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTT                                            >  SRR4302540.2187426/1‑94 (MQ=60)
                                                  GTCTTGCGCGACCTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTA                                           <  SRR4302540.1618165/94‑1 (MQ=60)
                                                    CTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAA                                         <  SRR4302540.8869172/94‑1 (MQ=60)
                                                    CTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAA                                         >  SRR4302540.2497394/1‑94 (MQ=60)
                                                    CTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAA                                         >  SRR4302540.4154338/1‑94 (MQ=60)
                                                       TCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAA                                      <  SRR4302540.1586148/94‑1 (MQ=60)
                                                          CGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATC                                   >  SRR4302540.1727617/1‑94 (MQ=60)
                                                          CGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATC                                   >  SRR4302540.2261734/1‑94 (MQ=60)
                                                          CGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATC                                   <  SRR4302540.9310507/94‑1 (MQ=60)
                                                           GACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCA                                  >  SRR4302540.8856212/1‑94 (MQ=60)
                                                            ACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAT                                 <  SRR4302540.6060484/94‑1 (MQ=60)
                                                            ACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAT                                 <  SRR4302540.9103643/94‑1 (MQ=60)
                                                               TGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGA                              <  SRR4302540.376612/94‑1 (MQ=60)
                                                               TGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGA                              <  SRR4302540.10091213/94‑1 (MQ=60)
                                                                GCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAA                             <  SRR4302540.2849664/94‑1 (MQ=60)
                                                                GCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGCGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAA                             <  SRR4302540.8332327/94‑1 (MQ=60)
                                                                  GTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATaagcgctagatcggaagagcggttcagcaggaa                           >  SRR4302540.5279881/1‑61 (MQ=60)
                                                                  GTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGT                           <  SRR4302540.9808895/94‑1 (MQ=60)
                                                                  GTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGA                           >  SRR4302540.3222633/1‑94 (MQ=60)
                                                                     ACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAG                        >  SRR4302540.1906230/1‑94 (MQ=60)
                                                                      CCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGCGCATTACGCTGCGATGGATCTCGGCGTAGTTAAAGAAATCATGGtagttggc                       >  SRR4302540.8677118/1‑86 (MQ=60)
                                                                        ACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACT                     <  SRR4302540.941958/94‑1 (MQ=60)
                                                                        ACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACT                     <  SRR4302540.4299064/94‑1 (MQ=60)
                                                                         CGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTG                    >  SRR4302540.9167893/1‑94 (MQ=60)
                                                                         CGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTG                    <  SRR4302540.3753653/94‑1 (MQ=60)
                                                                          GGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGC                   >  SRR4302540.193606/1‑94 (MQ=60)
                                                                           GTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCT                  >  SRR4302540.614892/1‑94 (MQ=60)
                                                                            TGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTT                 >  SRR4302540.319851/1‑94 (MQ=60)
                                                                            TGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTT                 >  SRR4302540.383271/1‑94 (MQ=60)
                                                                            TGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTT                 >  SRR4302540.1435349/1‑94 (MQ=60)
                                                                            TGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTT                 <  SRR4302540.625525/94‑1 (MQ=60)
                                                                            TGATATCGTCCACCCAGGTGTTCGGCGTGGTGTTGAGCATTACGCTGCGATGGATCCCGGCCTAGTTAcaacgatcatagaagtcagcttgctt                 >  SRR4302540.3284534/1‑68 (MQ=60)
                                                                             GATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTT                >  SRR4302540.1020380/1‑94 (MQ=60)
                                                                              ATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTT               <  SRR4302540.1276486/94‑1 (MQ=60)
                                                                                ATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTT             >  SRR4302540.6094469/1‑94 (MQ=60)
                                                                                ATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTC             >  SRR4302540.3572420/1‑94 (MQ=60)
                                                                                ATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTC             <  SRR4302540.4770236/94‑1 (MQ=60)
                                                                                 TCGTCCGCCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATTGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCT            <  SRR4302540.5349750/94‑1 (MQ=60)
                                                                                  CGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTT           <  SRR4302540.1539910/94‑1 (MQ=60)
                                                                                  CGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTT           <  SRR4302540.1632455/94‑1 (MQ=60)
                                                                                   GTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTG          >  SRR4302540.9611905/1‑94 (MQ=60)
                                                                                   GTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTG          >  SRR4302540.7503716/1‑94 (MQ=60)
                                                                                   GTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTC          >  SRR4302540.4730167/1‑94 (MQ=60)
                                                                                    TCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGC         >  SRR4302540.956520/1‑94 (MQ=60)
                                                                                       ACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT      >  SRR4302540.599709/1‑94 (MQ=60)
                                                                                       ACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT      >  SRR4302540.4076129/1‑94 (MQ=60)
                                                                                        CCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTT     <  SRR4302540.15184/94‑1 (MQ=60)
                                                                                         CCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTT    <  SRR4302540.7387724/94‑1 (MQ=60)
                                                                                         CCAGGTGTTCGGCGTGGTGTAGAGCAGTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTT    <  SRR4302540.2824747/94‑1 (MQ=60)
                                                                                          CGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT   >  SRR4302540.1167560/1‑94 (MQ=60)
                                                                                           GGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  <  SRR4302540.9672093/94‑1 (MQ=60)
                                                                                           GGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  <  SRR4302540.3443980/94‑1 (MQ=60)
                                                                                           |                                                                                             
CTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTC  >  NC_000913/1693458‑1693642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑