Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,310	T→C	Q252R (CAG→CGG)	hdhA ←	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,310	0	T	C	99.6%	763.4 / ‑5.0	240	Q252R (CAG→CGG)	hdhA	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (129/110); total (130/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 248 total aligned reads displayed.

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       >  2:9288195/1‑93 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       <  2:9207013/93‑1 (MQ=255)

   gTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAcc                                                                                      >  2:4045430/1‑93 (MQ=255)

     tATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAcccc                                                                                    >  2:4319675/1‑93 (MQ=255)

      aTTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCcca                                                                                   >  2:678995/1‑93 (MQ=255)

      aTTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCcca                                                                                   >  1:5272744/1‑93 (MQ=255)

         tAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCccacca                                                                                <  2:3302214/93‑1 (MQ=255)

           gCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCaccacc                                                                              <  1:1100355/93‑1 (MQ=255)

           gCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCaccacc                                                                              <  2:9071425/93‑1 (MQ=255)

           gCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCaccacc                                                                              >  1:978202/1‑93 (MQ=255)

           gCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCaccacc                                                                              <  1:4310277/93‑1 (MQ=255)

            cAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCg                                                                             >  1:4181609/1‑93 (MQ=255)

             agagACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCgg                                                                            >  1:1243396/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACACCACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTCCCCCACCACCGga                                                                           <  2:5320086/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  2:7877755/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:5014775/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  1:3557039/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  1:4015713/93‑1 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgagc                                                                         >  2:7547157/1‑92 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         <  1:6154683/93‑1 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         >  2:1259707/1‑93 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         >  2:5903437/1‑93 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         >  1:6613978/1‑93 (MQ=255)

                aaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         <  1:2198970/92‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCCGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGCGACGGt                                                                     >  1:4716500/1‑93 (MQ=255)

                    ttGATGAGGTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGGCCGTTATTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     >  2:3537290/1‑93 (MQ=255)

                     tgatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTg                                                                    <  2:9144516/93‑1 (MQ=255)

                     tgatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTg                                                                    <  1:3936299/93‑1 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGGCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCCGAGACGGTgg                                                                   >  2:100685/1‑92 (MQ=255)

                       atgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgag                                                                  >  1:9031800/1‑93 (MQ=255)

                       atgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgag                                                                  >  1:2802111/1‑93 (MQ=255)

                        tgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgaga                                                                 <  2:8908432/93‑1 (MQ=255)

                        tgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgaga                                                                 >  1:7266333/1‑93 (MQ=255)

                          aGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAt                                                               >  1:6996536/1‑93 (MQ=255)

                              tATCCCGGTAACCAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGt                                                           <  2:4216912/93‑1 (MQ=255)

                              tATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGt                                                           <  2:2136814/93‑1 (MQ=255)

                               aTCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTc                                                          >  1:3424845/1‑93 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         >  2:4655306/1‑93 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCACGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         <  1:1832658/93‑1 (MQ=255)

                                 cccGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGGCCg                                                        >  1:385255/1‑93 (MQ=255)

                                  ccGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGc                                                       >  1:8414589/1‑93 (MQ=255)

                                  ccGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGc                                                       >  2:4788521/1‑93 (MQ=255)

                                   cGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCt                                                      >  1:5353274/1‑93 (MQ=255)

                                    ggTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCtt                                                     <  1:9370079/93‑1 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTTTTAATTGAGCTTCCGTACCATACCACCGGAGACGGTCAGAATTTGTCCGCTTa                                                    >  2:8288808/1‑93 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCttc                                                    >  2:9099549/1‑92 (MQ=255)

                                      tAAACAAAACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAc                                                   >  2:4535470/1‑93 (MQ=255)

                                        aacaacACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAccc                                                 >  1:5211347/1‑93 (MQ=255)

                                        aacaacACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAccc                                                 <  1:2510562/93‑1 (MQ=255)

                                          caacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAg                                               >  2:8151118/1‑93 (MQ=255)

                                          caacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAg                                               >  2:6991142/1‑93 (MQ=255)

                                           aacaCGCACGGTTGTTTACCGGTACGTTAGAGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCCGc                                              >  1:7518845/1‑93 (MQ=255)

                                                  ccgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgcagc                                       <  1:2142517/92‑1 (MQ=255)

                                                   cgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgcagca                                      >  1:1518891/1‑93 (MQ=255)

                                                   cgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgcagca                                      <  1:9239820/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  1:7367622/93‑1 (MQ=255)

                                                     gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAgg                                    >  2:2402795/1‑93 (MQ=255)

                                                     gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAgg                                    <  1:2043591/93‑1 (MQ=255)

                                                     gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAgg                                    <  2:7881045/93‑1 (MQ=255)

                                                       tgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCg                                  <  2:8014730/93‑1 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:2265956/1‑93 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:140270/1‑93 (MQ=255)

                                                         tttCCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAg                                <  1:8248099/93‑1 (MQ=255)

                                                          ttACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGc                               <  1:6485689/93‑1 (MQ=255)

                                                          ttACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGc                               <  2:7236885/93‑1 (MQ=255)

                                                            aCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCaa                             >  2:5712702/1‑93 (MQ=255)

                                                             ccGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCaaa                            <  2:1981655/93‑1 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg                          >  1:3812081/1‑93 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg                          >  1:1969506/1‑93 (MQ=255)

                                                                gtgcGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         <  2:6884588/90‑1 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         >  1:1002466/1‑93 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         <  2:6917405/93‑1 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         >  1:3248424/1‑93 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         >  1:7854004/1‑93 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         <  1:969501/93‑1 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaaa                       >  2:3092849/1‑92 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAc                       >  1:9438890/1‑93 (MQ=255)

                                                                   cGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACa                      >  1:5970667/1‑93 (MQ=255)

                                                                   cGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACa                      <  2:1436895/93‑1 (MQ=255)

                                                                    gTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAg                     >  1:89188/1‑93 (MQ=255)

                                                                     ttAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc                    <  1:489911/93‑1 (MQ=255)

                                                                     ttAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc                    >  2:1793557/1‑93 (MQ=255)

                                                                     ttAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc                    >  1:1567776/1‑93 (MQ=255)

                                                                      tAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcg                   >  2:8818771/1‑93 (MQ=255)

                                                                        gtgtATTAATTGAGCTCCCGTGCCCCACCACCGTAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGAGAGCAAAGGAACAGCGCt                 <  1:1369938/93‑1 (MQ=255)

                                                                        gtgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCt                 >  1:515125/1‑93 (MQ=255)

                                                                         tgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGCACAGCGCTg                >  2:8456047/1‑93 (MQ=255)

                                                                           tATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCg              >  1:9856026/1‑93 (MQ=255)

                                                                              tAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTa           >  1:5713083/1‑93 (MQ=255)

                                                                                aTTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGc         >  1:547907/1‑93 (MQ=255)

                                                                                 ttGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCa        <  2:9244812/93‑1 (MQ=255)

                                                                                  tGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCaa       <  2:8699459/93‑1 (MQ=255)

                                                                                    aGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAata     <  2:1123996/93‑1 (MQ=255)

                                                                                    aGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAata     <  1:4548441/93‑1 (MQ=255)

                                                                                    aGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAata     >  2:8559788/1‑93 (MQ=255)

                                                                                     gCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAatat    >  1:7955081/1‑93 (MQ=255)

                                                                                     gCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAatat    <  2:160012/93‑1 (MQ=255)

                                                                                     gCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAatat    <  1:3119185/93‑1 (MQ=255)

                                                                                      cTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATc   <  2:3013226/93‑1 (MQ=255)

                                                                                       tCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCt  <  2:5989380/93‑1 (MQ=255)

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  NC_000913/1695217‑1695403

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCC                                                                                               >  SRR4302540.323556/1‑94 (MQ=60)

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCC                                                                                               <  SRR4302540.3999515/94‑1 (MQ=60)

 TGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCG                                                                                              >  SRR4302540.1894227/1‑94 (MQ=60)

 TGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCG                                                                                              <  SRR4302540.617489/94‑1 (MQ=60)

 TGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCG                                                                                              >  SRR4302540.7049993/1‑94 (MQ=60)

  GCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGT                                                                                             >  SRR4302540.9513885/1‑94 (MQ=60)

  GCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCACGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGT                                                                                             <  SRR4302540.6503852/94‑1 (MQ=60)

       TTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCC                                                                                        >  SRR4302540.9940664/1‑94 (MQ=60)

        TATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCA                                                                                       <  SRR4302540.2073691/94‑1 (MQ=60)

        TATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCA                                                                                       <  SRR4302540.1256196/94‑1 (MQ=60)

        TATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCA                                                                                       >  SRR4302540.5330298/1‑94 (MQ=60)

        TATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCA                                                                                       >  SRR4302540.5330299/1‑94 (MQ=60)

         ATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTCCCCCAC                                                                                      >  SRR4302540.7589800/1‑94 (MQ=60)

         ATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCAC                                                                                      >  SRR4302540.9609511/1‑94 (MQ=60)

          TTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACC                                                                                     <  SRR4302540.6614232/94‑1 (MQ=60)

           TTAGCATAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCA                                                                                    <  SRR4302540.6965369/94‑1 (MQ=60)

             AGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACC                                                                                  >  SRR4302540.988233/1‑94 (MQ=60)

              GCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGTTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCG                                                                                 <  SRR4302540.4973804/94‑1 (MQ=60)

              GCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCG                                                                                 >  SRR4302540.4227036/1‑94 (MQ=60)

              GCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCG                                                                                 <  SRR4302540.4357196/94‑1 (MQ=60)

              GCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCA                                                                                 <  SRR4302540.1111687/94‑1 (MQ=60)

               CAGAGACGTTGATTAGTTTATCCCGGTAAACAACACGCACGGTTTTTTGCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCCCCACCGG                                                                                <  SRR4302540.5189159/94‑1 (MQ=60)

               CAGAGACGTTGATGAGTTTATCCCGGTTAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCTCGTACCCCACCACCGG                                                                                >  SRR4302540.3996752/1‑94 (MQ=60)

               CAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGG                                                                                >  SRR4302540.3903656/1‑94 (MQ=60)

               CAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGG                                                                                >  SRR4302540.1256078/1‑94 (MQ=60)

                AGAGACGTTGATGAGTTTATCCCTGTAAACAACACGCACGGATGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGA                                                                               >  SRR4302540.3965765/1‑94 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              <  SRR4302540.4058968/94‑1 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              <  SRR4302540.3595905/94‑1 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              <  SRR4302540.3438282/94‑1 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              >  SRR4302540.4806269/1‑94 (MQ=60)

                  AGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGA                                                                             >  SRR4302540.6687233/1‑94 (MQ=60)

                   GACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAC                                                                            <  SRR4302540.6223249/94‑1 (MQ=60)

                   GACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAC                                                                            <  SRR4302540.6223250/94‑1 (MQ=60)

                   AACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAC                                                                            <  SRR4302540.2222716/94‑1 (MQ=60)

                    ACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACG                                                                           <  SRR4302540.6505007/94‑1 (MQ=60)

                     CGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGG                                                                          >  SRR4302540.5924140/1‑94 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGT                                                                         <  SRR4302540.9939634/94‑1 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCCGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGCGACGGT                                                                         >  SRR4302540.4766892/1‑94 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       <  SRR4302540.3978840/94‑1 (MQ=60)

                         GATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAG                                                                      >  SRR4302540.2832379/1‑94 (MQ=60)

                         GATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAG                                                                      >  SRR4302540.9140187/1‑94 (MQ=60)

                          ATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGA                                                                     >  SRR4302540.7347408/1‑94 (MQ=60)

                            GAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAT                                                                   >  SRR4302540.7074191/1‑94 (MQ=60)

                            GAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAT                                                                   <  SRR4302540.3401229/94‑1 (MQ=60)

                             AGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTTGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATT                                                                  >  SRR4302540.5911259/1‑94 (MQ=60)

                             AGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATT                                                                  >  SRR4302540.10077204/1‑94 (MQ=60)

                              GTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTT                                                                 >  SRR4302540.3559778/1‑94 (MQ=60)

                              GTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTT                                                                 >  SRR4302540.2852957/1‑94 (MQ=60)

                               TTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTG                                                                <  SRR4302540.4066568/94‑1 (MQ=60)

                               TTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTG                                                                >  SRR4302540.2690124/1‑94 (MQ=60)

                                 TATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTC                                                              >  SRR4302540.5021323/1‑94 (MQ=60)

                                 TATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTC                                                              >  SRR4302540.3462078/1‑94 (MQ=60)

                                 TATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTC                                                              <  SRR4302540.1404706/94‑1 (MQ=60)

                                  ATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCC                                                             <  SRR4302540.8870580/94‑1 (MQ=60)

                                   TCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGGCCG                                                            >  SRR4302540.389235/1‑94 (MQ=60)

                                   TCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCACGTACCCCACCACCGGAGACGGTGAGAATTTGTCCG                                                            <  SRR4302540.1852709/94‑1 (MQ=60)

                                    CCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGC                                                           >  SRR4302540.8512259/1‑94 (MQ=60)

                                     CCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCT                                                          >  SRR4302540.5412050/1‑94 (MQ=60)

                                     CCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCT                                                          >  SRR4302540.9477461/1‑94 (MQ=60)

                                      CGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTT                                                         >  SRR4302540.7406428/1‑94 (MQ=60)

                                       GGTAAACAACACGCACGTTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTA                                                        <  SRR4302540.2040977/94‑1 (MQ=60)

                                       GGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTA                                                        <  SRR4302540.9481641/94‑1 (MQ=60)

                                         TAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACC                                                      >  SRR4302540.2179487/1‑94 (MQ=60)

                                          AAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCC                                                     >  SRR4302540.151775/1‑94 (MQ=60)

                                          AAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCC                                                     <  SRR4302540.68678/94‑1 (MQ=60)

                                          AAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCC                                                     >  SRR4302540.5268128/1‑94 (MQ=60)

                                          AAACAACACGCACGGGTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCAACACAGGAGACGGTGAGAATTTGTCCGCTTACCA                                                     >  SRR4302540.151774/1‑94 (MQ=60)

                                           AACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCA                                                    <  SRR4302540.4109542/94‑1 (MQ=60)

                                           AACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCA                                                    <  SRR4302540.912297/94‑1 (MQ=60)

                                           AACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCA                                                    <  SRR4302540.2537676/94‑1 (MQ=60)

                                             CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGC                                                  >  SRR4302540.7699079/1‑94 (MQ=60)

                                             CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGC                                                  >  SRR4302540.4760503/1‑94 (MQ=60)

                                             CAACACGCACGGTTGTTTACCGGTACGTTAGAGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCCGC                                                  >  SRR4302540.7602923/1‑94 (MQ=60)

                                              AACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCT                                                 >  SRR4302540.3860992/1‑94 (MQ=60)

                                                 ACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGC                                              <  SRR4302540.5386014/94‑1 (MQ=60)

                                                     CCGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCA                                          <  SRR4302540.2165540/94‑1 (MQ=60)

                                                     ACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCA                                          >  SRR4302540.1535697/1‑94 (MQ=60)

                                                      CGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAG                                         <  SRR4302540.9350500/94‑1 (MQ=60)

                                                      CGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAG                                         >  SRR4302540.9534746/1‑94 (MQ=60)

                                                       GGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGG                                        <  SRR4302540.7449760/94‑1 (MQ=60)

                                                       GGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGG                                        >  SRR4302540.5655337/1‑94 (MQ=60)

                                                        GTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGC                                       <  SRR4302540.2065604/94‑1 (MQ=60)

                                                        GTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGC                                       <  SRR4302540.9236893/94‑1 (MQ=60)

                                                         TTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCG                                      <  SRR4302540.9205912/94‑1 (MQ=60)

                                                         TTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCG                                      >  SRR4302540.6112849/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302540.858285/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302540.1324230/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302540.141731/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302540.175031/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302540.2290273/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     <  SRR4302540.2188684/94‑1 (MQ=60)

                                                           GTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAG                                    >  SRR4302540.1535864/1‑94 (MQ=60)

                                                            TTTCCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGC                                   <  SRR4302540.8343969/94‑1 (MQ=60)

                                                            TTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGC                                   >  SRR4302540.1029215/1‑94 (MQ=60)

                                                             TTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCA                                  >  SRR4302540.7119562/1‑94 (MQ=60)

                                                             TTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCA                                  <  SRR4302540.6557818/94‑1 (MQ=60)

                                                                CCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAG                               <  SRR4302540.2459027/94‑1 (MQ=60)

                                                                CCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAG                               <  SRR4302540.8120265/94‑1 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGG                              <  SRR4302540.4312325/94‑1 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGG                              >  SRR4302540.1990887/1‑94 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGG                              >  SRR4302540.3853591/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302540.7945774/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302540.1012648/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302540.3031694/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302540.3283804/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             <  SRR4302540.4368769/94‑1 (MQ=60)

                                                                   GTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAA                            <  SRR4302540.979483/94‑1 (MQ=60)

                                                                   GTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAA                            >  SRR4302540.1852928/1‑94 (MQ=60)

                                                                   GTCCGTTAGTGCATTAATTGAGCTTCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAAGAA                            >  SRR4302540.3849860/1‑94 (MQ=60)

                                                                    TCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAC                           >  SRR4302540.9550979/1‑94 (MQ=60)

                                                                     CCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          >  SRR4302540.6036751/1‑94 (MQ=60)

                                                                     CCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          <  SRR4302540.1188818/94‑1 (MQ=60)

                                                                     CCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          <  SRR4302540.1776709/94‑1 (MQ=60)

                                                                     CCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          <  SRR4302540.2410422/94‑1 (MQ=60)

                                                                     CCGTTAGTGTAGTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          <  SRR4302540.4571330/94‑1 (MQ=60)

                                                                      CGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAG                         <  SRR4302540.3624073/94‑1 (MQ=60)

                                                                      CGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAG                         >  SRR4302540.90127/1‑94 (MQ=60)

                                                                       GTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGC                        >  SRR4302540.1584972/1‑94 (MQ=60)

                                                                       GTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGC                        <  SRR4302540.5295218/94‑1 (MQ=60)

                                                                        TTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG                       >  SRR4302540.7824244/1‑94 (MQ=60)

                                                                        TTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG                       <  SRR4302540.495160/94‑1 (MQ=60)

                                                                          AGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCT                     >  SRR4302540.520651/1‑94 (MQ=60)

                                                                           GTGTATTAATTGAGCTCCCGTGCCCCACCACCGTAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGAGAGCAAAGGAACAGCGCTG                    <  SRR4302540.1384456/94‑1 (MQ=60)

                                                                           GTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTG                    >  SRR4302540.2047149/1‑94 (MQ=60)

                                                                            TGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGC                   >  SRR4302540.5258002/1‑94 (MQ=60)

                                                                             GTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCG                  >  SRR4302540.9971211/1‑94 (MQ=60)

                                                                                TTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTA               >  SRR4302540.5775972/1‑94 (MQ=60)

                                                                                  AATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGC             >  SRR4302540.8484577/1‑94 (MQ=60)

                                                                                  AATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGC             >  SRR4302540.553875/1‑94 (MQ=60)

                                                                                    TTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAA           >  SRR4302540.2282678/1‑94 (MQ=60)

                                                                                    TTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAA           <  SRR4302540.9872216/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302540.5437441/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302540.4597642/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        >  SRR4302540.8047813/1‑94 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        >  SRR4302540.8047814/1‑94 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302540.5437440/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302540.8116098/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302540.7376259/94‑1 (MQ=60)

                                                                                        GCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATC       <  SRR4302540.3153278/94‑1 (MQ=60)

                                                                                         CTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT      >  SRR4302540.3180453/1‑94 (MQ=60)

                                                                                          TCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTT     >  SRR4302540.1518720/1‑94 (MQ=60)

                                                                                           CCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTG    <  SRR4302540.9433334/94‑1 (MQ=60)

                                                                                           CCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTG    <  SRR4302540.2180176/94‑1 (MQ=60)

                                                                                             CGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  SRR4302540.1522999/1‑94 (MQ=60)

                                                                                             CGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  <  SRR4302540.6414332/94‑1 (MQ=60)

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  NC_000913/1695217‑1695403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`