Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,310	T→C	Q252R (CAG→CGG)	hdhA ←	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,310	0	T	C	100.0%	646.6 / NA	201	Q252R (CAG→CGG)	hdhA	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (91/110); total (91/110)

Only 100 of 204 total aligned reads displayed.

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

 cAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGGCCGTTAGTGTATTAATTGAGCTCCCGTa                                                                                        >  1:4781128/1‑93 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       >  1:4203861/1‑93 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       <  2:3619878/93‑1 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       <  2:5025848/93‑1 (MQ=255)

     tATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAcccc                                                                                    >  1:5008945/1‑93 (MQ=255)

       tttAGCAGAGATTTTGATGAGTTGATCCAGGTAAACAACACGCACGGTTGTTTACCGGTCCGTGAGTGTATTAATTGAGCTCCCGTACCccac                                                                                  <  2:2011010/93‑1 (MQ=255)

       tttAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCccac                                                                                  <  2:3218034/93‑1 (MQ=255)

         tAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCccacca                                                                                <  1:1244244/93‑1 (MQ=255)

         tAGAAGAGACGTAGATGAGTTTATCCCGGTAAACAACACGCACGATTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTTCCccacca                                                                                <  2:4940101/93‑1 (MQ=255)

          aGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCcaccac                                                                               <  1:178791/93‑1 (MQ=255)

          aGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCcaccac                                                                               <  1:3043317/93‑1 (MQ=255)

             agagACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCgg                                                                            >  2:1949424/1‑93 (MQ=255)

             agagACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCgg                                                                            <  1:3377701/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:3711063/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  2:5837772/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:1633113/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:4217876/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  2:5895307/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGCAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGGATTAATTGAGCTCCCGTACCCCACCAACGga                                                                           >  2:3810496/1‑93 (MQ=255)

               agaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgag                                                                          <  1:4950237/93‑1 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         <  1:5261101/93‑1 (MQ=255)

                 aCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAc                                                                        <  2:4236591/93‑1 (MQ=255)

                   gTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACgg                                                                      <  1:9618/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACggg                                                                     >  1:5658850/1‑92 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  1:1381671/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     >  1:6181606/1‑93 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  1:4342940/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     >  2:5802237/1‑93 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  2:5180719/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCACACCACCGGAGACggg                                                                     >  1:5133220/1‑92 (MQ=255)

                     tgatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTg                                                                    >  1:5789174/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:2940180/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:501455/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:47264/1‑93 (MQ=255)

                       atgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgag                                                                  <  1:3514079/93‑1 (MQ=255)

                        tgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgaga                                                                 <  2:783503/93‑1 (MQ=255)

                         gaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGaa                                                                <  2:170462/93‑1 (MQ=255)

                          aGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAt                                                               <  2:4681944/93‑1 (MQ=255)

                            tttATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAttt                                                             <  1:1956730/93‑1 (MQ=255)

                                tCCCGGTAAACACCACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         <  2:3569094/93‑1 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         >  2:604997/1‑93 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         <  2:458822/93‑1 (MQ=255)

                                   cGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCt                                                      >  1:4556853/1‑93 (MQ=255)

                                    ggTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGCGACGGTGAGAATTTGTCCGCtt                                                     >  2:1148201/1‑93 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTa                                                    <  1:5300301/93‑1 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTa                                                    >  1:3232266/1‑93 (MQ=255)

                                      tAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAc                                                   <  2:4773131/93‑1 (MQ=255)

                                      aaaaCAACACGCACGGTGGTTAGCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTGCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAc                                                   <  1:3930713/92‑1 (MQ=255)

                                       aaaCAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAcc                                                  >  2:6244168/1‑93 (MQ=255)

                                        aacaacACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAccc                                                 <  1:2675517/93‑1 (MQ=255)

                                         acaacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCa                                                >  2:3964748/1‑93 (MQ=255)

                                            acacGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCt                                             >  1:581593/1‑93 (MQ=255)

                                            acacGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCt                                             <  1:6034209/93‑1 (MQ=255)

                                                gcacgGTTGTTAGCAGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgca                                         <  2:2694390/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGTCGGTGAGACTTTGTCCGCTTAGCCAGCTCGccgcag                                     >  2:2312791/1‑93 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  2:3097182/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  2:3889893/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  1:3149650/93‑1 (MQ=255)

                                                     gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAgg                                    <  1:1482315/93‑1 (MQ=255)

                                                      ttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGc                                   <  2:4452270/93‑1 (MQ=255)

                                                      ttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGc                                   <  1:5972053/93‑1 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  2:5536953/1‑93 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:851013/1‑93 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:2332906/1‑93 (MQ=255)

                                                             ccGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCaaa                            <  1:2438806/93‑1 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGCGCACAgg                          >  1:4820006/1‑93 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg                          >  2:2972282/1‑93 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         >  1:3296312/1‑93 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        >  2:2209200/1‑93 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  1:1968278/93‑1 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  2:3318159/93‑1 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  1:3683985/93‑1 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAc                       >  1:1050116/1‑93 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAACTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAc                       <  2:5135054/93‑1 (MQ=255)

                                                                   cGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACa                      <  1:1562079/93‑1 (MQ=255)

                                                                    gTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAg                     >  1:4597774/1‑93 (MQ=255)

                                                                     ttAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc                    <  2:3114575/93‑1 (MQ=255)

                                                                      tAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcg                   >  1:648174/1‑93 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCTCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  2:663415/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  1:4247851/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  >  2:2142503/1‑93 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  1:2864612/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  2:565188/93‑1 (MQ=255)

                                                                        gtgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCt                 <  1:2221373/93‑1 (MQ=255)

                                                                         tgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGATTTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTg                <  1:2044513/93‑1 (MQ=255)

                                                                          gtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGc               <  1:5594050/93‑1 (MQ=255)

                                                                          gtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGc               >  1:3087388/1‑93 (MQ=255)

                                                                            aTTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGt             <  2:4099335/93‑1 (MQ=255)

                                                                             ttAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGtt            >  1:1915787/1‑93 (MQ=255)

                                                                             ttAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGtt            <  2:1909318/93‑1 (MQ=255)

                                                                              tAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTa           <  1:1633113/93‑1 (MQ=255)

                                                                                 ttGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCa        <  2:1483719/93‑1 (MQ=255)

                                                                                  tGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCaa       <  2:2611780/93‑1 (MQ=255)

                                                                                  tGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCaa       >  2:6146802/1‑93 (MQ=255)

                                                                                   gAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat      <  1:2558072/93‑1 (MQ=255)

                                                                                    aGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAata     <  2:5924762/93‑1 (MQ=255)

                                                                                      cTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATc   <  2:1663514/93‑1 (MQ=255)

                                                                                      cTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATc   <  2:4458802/93‑1 (MQ=255)

                                                                                       tCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCt  <  1:4480070/93‑1 (MQ=255)

                                                                                       tCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCt  >  1:168102/1‑93 (MQ=255)

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  NC_000913/1695217‑1695403

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGTTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCC                                                                                               <  SRR4302533.4578540/94‑1 (MQ=60)

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCC                                                                                               >  SRR4302533.2307064/1‑94 (MQ=60)

  GCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGT                                                                                             <  SRR4302533.1471677/94‑1 (MQ=60)

  GCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGT                                                                                             >  SRR4302533.4723058/1‑94 (MQ=60)

   CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGGCCGTTAGTGTATTAATTGAGCTCCCGTA                                                                                            >  SRR4302533.4810096/1‑94 (MQ=60)

    CAGTTATTTAGCAGAGACGTTGATGATTTTACCCCGGTAACCAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAC                                                                                           <  SRR4302533.3364738/94‑1 (MQ=60)

    CAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAC                                                                                           >  SRR4302533.4229291/1‑94 (MQ=60)

    CAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAC                                                                                           <  SRR4302533.601417/94‑1 (MQ=60)

     AGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACC                                                                                          <  SRR4302533.255349/94‑1 (MQ=60)

       TTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCC                                                                                        >  SRR4302533.5039293/1‑94 (MQ=60)

        TATTAAGCAGAGACTTTGATGATTTTACCCCGGTAAACAACACGCACGGTTTTTTCCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCA                                                                                       <  SRR4302533.3308951/94‑1 (MQ=60)

           TTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCA                                                                                    <  SRR4302533.4583366/94‑1 (MQ=60)

            TAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCAC                                                                                   <  SRR4302533.1251674/94‑1 (MQ=60)

             AGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACC                                                                                  <  SRR4302533.3061947/94‑1 (MQ=60)

             AGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACC                                                                                  >  SRR4302533.1975102/1‑94 (MQ=60)

             AGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACC                                                                                  <  SRR4302533.179909/94‑1 (MQ=60)

                AGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGA                                                                               >  SRR4302533.3109005/1‑94 (MQ=60)

                AGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGA                                                                               >  SRR4302533.4996526/1‑94 (MQ=60)

                AGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGA                                                                               <  SRR4302533.3398554/94‑1 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTGCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              <  SRR4302533.4484325/94‑1 (MQ=60)

                 GAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAG                                                                              <  SRR4302533.800137/94‑1 (MQ=60)

                  AGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGA                                                                             <  SRR4302533.4980218/94‑1 (MQ=60)

                   GACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAC                                                                            <  SRR4302533.1689501/94‑1 (MQ=60)

                   GACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAC                                                                            <  SRR4302533.5292686/94‑1 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGT                                                                         <  SRR4302533.9699/94‑1 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGT                                                                         <  SRR4302533.3451294/94‑1 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGT                                                                         >  SRR4302533.6218727/1‑94 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGG                                                                         >  SRR4302533.5693044/1‑94 (MQ=60)

                      GTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCACACCACCGGAGACGGG                                                                         >  SRR4302533.5164230/1‑94 (MQ=60)

                       TTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTG                                                                        >  SRR4302533.5040477/1‑94 (MQ=60)

                       TTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTG                                                                        >  SRR4302533.5824153/1‑94 (MQ=60)

                       TTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTG                                                                        <  SRR4302533.4369113/94‑1 (MQ=60)

                       TTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTG                                                                        <  SRR4302533.1389989/94‑1 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       >  SRR4302533.47591/1‑94 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       >  SRR4302533.504437/1‑94 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       >  SRR4302533.1615130/1‑94 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       <  SRR4302533.1563718/94‑1 (MQ=60)

                        TGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGA                                                                       >  SRR4302533.2958139/1‑94 (MQ=60)

                          ATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGA                                                                     <  SRR4302533.3535741/94‑1 (MQ=60)

                             AGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATT                                                                  <  SRR4302533.5840770/94‑1 (MQ=60)

                               TTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTG                                                                <  SRR4302533.1968671/94‑1 (MQ=60)

                                  ATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCC                                                             <  SRR4302533.5273203/94‑1 (MQ=60)

                                  ATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCC                                                             <  SRR4302533.4829063/94‑1 (MQ=60)

                                    CCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGC                                                           >  SRR4302533.199160/1‑94 (MQ=60)

                                     CCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCT                                                          >  SRR4302533.4584489/1‑94 (MQ=60)

                                       GGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTA                                                        >  SRR4302533.3252184/1‑94 (MQ=60)

                                        GTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAC                                                       <  SRR4302533.5332054/94‑1 (MQ=60)

                                         TAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACC                                                      >  SRR4302533.2619946/1‑94 (MQ=60)

                                         AAAACAACACGCACGGTGGTTAGCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTGCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACC                                                      <  SRR4302533.3954708/94‑1 (MQ=60)

                                           AACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCA                                                    <  SRR4302533.2691927/94‑1 (MQ=60)

                                              AACACGCACGGTTGTTTACCGGTCCGTTTGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCT                                                 >  SRR4302533.1847175/1‑94 (MQ=60)

                                              AACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCT                                                 >  SRR4302533.585021/1‑94 (MQ=60)

                                               ACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTC                                                >  SRR4302533.3211661/1‑94 (MQ=60)

                                               ACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTC                                                <  SRR4302533.6070776/94‑1 (MQ=60)

                                                 ACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGC                                              >  SRR4302533.4884526/1‑94 (MQ=60)

                                                     ACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCA                                          >  SRR4302533.3836933/1‑94 (MQ=60)

                                                       GGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGG                                        <  SRR4302533.3169012/94‑1 (MQ=60)

                                                       GGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGG                                        >  SRR4302533.3094387/1‑94 (MQ=60)

                                                       GGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGG                                        <  SRR4302533.1936435/94‑1 (MQ=60)

                                                        GTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGC                                       <  SRR4302533.1491265/94‑1 (MQ=60)

                                                         TTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCG                                      <  SRR4302533.5477492/94‑1 (MQ=60)

                                                         TTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCG                                      <  SRR4302533.6008202/94‑1 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302533.855994/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302533.5102560/1‑94 (MQ=60)

                                                          TGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGA                                     >  SRR4302533.2347350/1‑94 (MQ=60)

                                                           GTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAG                                    <  SRR4302533.5822204/94‑1 (MQ=60)

                                                            TTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGC                                   >  SRR4302533.2798844/1‑94 (MQ=60)

                                                              TACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAA                                 >  SRR4302533.2175081/1‑94 (MQ=60)

                                                               ACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAA                                <  SRR4302533.13540/94‑1 (MQ=60)

                                                                CCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAG                               <  SRR4302533.2453866/94‑1 (MQ=60)

                                                                CCCGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAG                               <  SRR4302533.5319800/94‑1 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGCGCACAGG                              >  SRR4302533.4849213/1‑94 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGG                              <  SRR4302533.6223225/94‑1 (MQ=60)

                                                                 CGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGG                              >  SRR4302533.640508/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302533.872994/1‑94 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             <  SRR4302533.430303/94‑1 (MQ=60)

                                                                  GGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGA                             >  SRR4302533.3316658/1‑94 (MQ=60)

                                                                   GTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAA                            <  SRR4302533.348798/94‑1 (MQ=60)

                                                                   GTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAA                            <  SRR4302533.4256200/94‑1 (MQ=60)

                                                                    TCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAC                           >  SRR4302533.1056289/1‑94 (MQ=60)

                                                                    TCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAC                           >  SRR4302533.1753723/1‑94 (MQ=60)

                                                                    TCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAC                           <  SRR4302533.1980313/94‑1 (MQ=60)

                                                                    TCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAC                           <  SRR4302533.3706627/94‑1 (MQ=60)

                                                                     CCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACA                          <  SRR4302533.4612332/94‑1 (MQ=60)

                                                                      CGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAG                         >  SRR4302533.4625626/1‑94 (MQ=60)

                                                                      CGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAG                         <  SRR4302533.1571482/94‑1 (MQ=60)

                                                                       GTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGC                        >  SRR4302533.1076220/1‑94 (MQ=60)

                                                                        TTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG                       >  SRR4302533.651996/1‑94 (MQ=60)

                                                                          AGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCT                     >  SRR4302533.4790151/1‑94 (MQ=60)

                                                                          AGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCT                     <  SRR4302533.4273490/94‑1 (MQ=60)

                                                                          AGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCT                     <  SRR4302533.2882233/94‑1 (MQ=60)

                                                                           GTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTG                    <  SRR4302533.2235092/94‑1 (MQ=60)

                                                                            TGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGATTTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGC                   <  SRR4302533.2057115/94‑1 (MQ=60)

                                                                            TGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGC                   >  SRR4302533.3106370/1‑94 (MQ=60)

                                                                             GTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCG                  >  SRR4302533.3652785/1‑94 (MQ=60)

                                                                             GTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCG                  <  SRR4302533.5627844/94‑1 (MQ=60)

                                                                              TATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGT                 >  SRR4302533.6217949/1‑94 (MQ=60)

                                                                               ATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTT                >  SRR4302533.4063556/1‑94 (MQ=60)

                                                                               ATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTT                >  SRR4302533.3037077/1‑94 (MQ=60)

                                                                               ATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTT                >  SRR4302533.1927482/1‑94 (MQ=60)

                                                                                 TAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAG              <  SRR4302533.1643003/94‑1 (MQ=60)

                                                                                  AATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGC             >  SRR4302533.2253189/1‑94 (MQ=60)

                                                                                   ATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCA            >  SRR4302533.5954821/1‑94 (MQ=60)

                                                                                      GAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATA         <  SRR4302533.2573800/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        <  SRR4302533.5675313/94‑1 (MQ=60)

                                                                                       AGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAAGCGAGCAAAGGAACAGCGCTGCGTTAGCAATAT        >  SRR4302533.2929286/1‑94 (MQ=60)

                                                                                         CTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT      >  SRR4302533.1460719/1‑94 (MQ=60)

                                                                                         CTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT      >  SRR4302533.169163/1‑94 (MQ=60)

                                                                                          TCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTT     <  SRR4302533.244362/94‑1 (MQ=60)

                                                                                          TCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTT     <  SRR4302533.4507173/94‑1 (MQ=60)

                                                                                           CCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTG    <  SRR4302533.4902611/94‑1 (MQ=60)

                                                                                            CCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGC   >  SRR4302533.1891472/1‑94 (MQ=60)

                                                                                            CCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGC   >  SRR4302533.2810311/1‑94 (MQ=60)

                                                                                            CCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGC   <  SRR4302533.1486636/94‑1 (MQ=60)

                                                                                             CGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  SRR4302533.1863299/1‑94 (MQ=60)

                                                                                             CGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  <  SRR4302533.988474/94‑1 (MQ=60)

                                                                                             CGTACCCCACCACCGGAGACGGTCAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  <  SRR4302533.5497112/94‑1 (MQ=60)

CTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCG  >  NC_000913/1695217‑1695403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`