Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,736,520	A→G	Q218R (CAG→CGG)	purR →	PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,736,520	0	A	G	100.0%	541.9 / NA	168	Q218R (CAG→CGG)	purR	PurR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (88/80); total (88/80)

Only 100 of 174 total aligned reads displayed.

TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCG  >  NC_000913/1736430‑1736609

tGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCggg                                                                                         <  1:754642/93‑1 (MQ=255)

tGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCggg                                                                                         >  2:179113/1‑93 (MQ=255)

 ggAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCgggg                                                                                        >  1:5455665/1‑93 (MQ=255)

 ggAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCgggg                                                                                        >  1:1647396/1‑93 (MQ=255)

 ggAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCgggg                                                                                        >  1:2332994/1‑93 (MQ=255)

   aaCGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTg                                                                                      >  1:767872/1‑93 (MQ=255)

    aCGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGa                                                                                     >  1:6082980/1‑93 (MQ=255)

     cGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGAc                                                                                    >  1:2982299/1‑93 (MQ=255)

      gTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACt                                                                                   >  1:2175068/1‑93 (MQ=255)

        aaCACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACttt                                                                                 <  2:6175977/93‑1 (MQ=255)

            ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAc                                                                             <  2:4695134/93‑1 (MQ=255)

             cGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAcc                                                                            >  2:1856621/1‑93 (MQ=255)

              ggCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCt                                                                           >  2:2998427/1‑93 (MQ=255)

               gcgcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTg                                                                          >  1:1750085/1‑93 (MQ=255)

               gcgcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTg                                                                          >  1:613349/1‑93 (MQ=255)

                 gcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGaa                                                                        <  1:5797614/93‑1 (MQ=255)

                 gcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGaa                                                                        <  1:1405820/93‑1 (MQ=255)

                  cAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAt                                                                       <  1:5979593/93‑1 (MQ=255)

                  cAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAt                                                                       >  1:5453425/1‑93 (MQ=255)

                  cAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAt                                                                       >  2:571582/1‑93 (MQ=255)

                   aGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATc                                                                      <  2:5683605/93‑1 (MQ=255)

                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                     <  2:390287/93‑1 (MQ=255)

                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                     >  2:2388364/1‑93 (MQ=255)

                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                     <  1:407697/93‑1 (MQ=255)

                     gccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCg                                                                    >  1:2067342/1‑93 (MQ=255)

                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                   <  1:4188092/93‑1 (MQ=255)

                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                   <  2:2419997/93‑1 (MQ=255)

                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGGGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                   <  1:1022365/93‑1 (MQ=255)

                       cgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGt                                                                  <  2:3749138/93‑1 (MQ=255)

                       cgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGt                                                                  <  1:3846468/93‑1 (MQ=255)

                         ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTa                                                                >  2:3642824/1‑93 (MQ=255)

                         ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTa                                                                <  2:5222230/93‑1 (MQ=255)

                         ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTa                                                                >  2:1545675/1‑93 (MQ=255)

                         ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTa                                                                >  1:3607863/1‑93 (MQ=255)

                          cTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAt                                                               >  1:4822988/1‑93 (MQ=255)

                          cTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAt                                                               >  1:5322264/1‑93 (MQ=255)

                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                              >  1:4592008/1‑93 (MQ=255)

                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                              >  1:3122565/1‑93 (MQ=255)

                            tGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATcg                                                             <  2:6271886/93‑1 (MQ=255)

                                ggTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGcc                                                         >  2:2877290/1‑93 (MQ=255)

                                ggTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGcc                                                         >  1:6153584/1‑93 (MQ=255)

                                 gTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCa                                                        >  1:128833/1‑93 (MQ=255)

                                 gTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCa                                                        >  1:366413/1‑93 (MQ=255)

                                  ttttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAt                                                       <  2:5723406/93‑1 (MQ=255)

                                  ttttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAt                                                       >  2:4064995/1‑93 (MQ=255)

                                    ttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgc                                                     <  1:4375680/93‑1 (MQ=255)

                                      aTGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcag                                                   >  2:3968640/1‑93 (MQ=255)

                                       tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcagc                                                  >  2:1141724/1‑93 (MQ=255)

                                       tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcaga                                                  >  2:920777/1‑92 (MQ=255)

                                        gAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcagca                                                 >  1:5811367/1‑93 (MQ=255)

                                        gAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcagca                                                 <  1:3978343/93‑1 (MQ=255)

                                         aaGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaa                                                >  2:3417873/1‑93 (MQ=255)

                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                               <  1:2507461/93‑1 (MQ=255)

                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                               >  1:523444/1‑93 (MQ=255)

                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                               <  1:4330029/93‑1 (MQ=255)

                                           ggCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaac                                              >  1:480662/1‑92 (MQ=255)

                                               aTGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTg                                          >  1:6020950/1‑93 (MQ=255)

                                               aTGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTg                                          <  2:1423305/93‑1 (MQ=255)

                                                 ggAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTc                                        <  1:6040898/93‑1 (MQ=255)

                                                 ggAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTc                                        <  1:5373940/93‑1 (MQ=255)

                                                  gaagaaGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCg                                       >  1:1211057/1‑93 (MQ=255)

                                                    agaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCa                                     >  2:793395/1‑93 (MQ=255)

                                                     gaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAg                                    <  1:3492801/93‑1 (MQ=255)

                                                      aagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGc                                   <  2:4155306/93‑1 (MQ=255)

                                                      aagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGc                                   >  2:2410888/1‑93 (MQ=255)

                                                           atgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                              >  1:2038289/1‑93 (MQ=255)

                                                           atgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                              <  2:1076903/93‑1 (MQ=255)

                                                           atgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                              <  2:2661286/93‑1 (MQ=255)

                                                           atgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                              <  2:327215/93‑1 (MQ=255)

                                                            tgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATc                             >  2:163261/1‑93 (MQ=255)

                                                             gatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCg                            >  2:5001360/1‑93 (MQ=255)

                                                             gatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCg                            >  2:3740866/1‑93 (MQ=255)

                                                              atCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGc                           <  1:1283720/93‑1 (MQ=255)

                                                                cAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGccc                         >  2:5883606/1‑93 (MQ=255)

                                                                cAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGccc                         >  2:3113472/1‑93 (MQ=255)

                                                                cAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGccc                         <  1:5765914/93‑1 (MQ=255)

                                                                 aaGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCt                        >  1:5270942/1‑93 (MQ=255)

                                                                 aaGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCt                        >  1:1821612/1‑93 (MQ=255)

                                                                 aaGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCt                        >  2:5554120/1‑93 (MQ=255)

                                                                   ggTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAc                      <  1:5652763/93‑1 (MQ=255)

                                                                   ggTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAc                      <  1:2639657/93‑1 (MQ=255)

                                                                    gTGCAGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCAGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCTCCCTACt                     <  1:1522117/93‑1 (MQ=255)

                                                                      gCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGc                   >  1:4301106/1‑93 (MQ=255)

                                                                        cGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCg                 >  1:288867/1‑93 (MQ=255)

                                                                        cGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCg                 >  1:2824882/1‑93 (MQ=255)

                                                                         ggAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGt                >  2:1344263/1‑93 (MQ=255)

                                                                            aaGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtctt             >  2:2837311/1‑93 (MQ=255)

                                                                            aaGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtctt             >  1:6063859/1‑93 (MQ=255)

                                                                             aGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc            >  1:493160/1‑93 (MQ=255)

                                                                             aGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc            <  1:6309698/93‑1 (MQ=255)

                                                                             aGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc            <  2:3254845/93‑1 (MQ=255)

                                                                             aGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc            <  1:5265467/93‑1 (MQ=255)

                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct           <  1:2561449/93‑1 (MQ=255)

                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct           >  2:6217177/1‑93 (MQ=255)

                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct           >  1:1684319/1‑93 (MQ=255)

                                                                               cTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTTTCGCAGCCGCATCGCCCTACTGCCGTCTTCtg          <  1:3985947/93‑1 (MQ=255)

                                                                               cTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtg          <  1:6295426/93‑1 (MQ=255)

                                                                                   gttGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggt      <  2:207711/92‑1 (MQ=255)

                                                                                   aTTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggt      <  1:1157535/93‑1 (MQ=255)

                                                                                    ttGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggtg     >  2:434135/1‑93 (MQ=255)

TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCG  >  NC_000913/1736430‑1736609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATAT  >  NC_000913/1736427‑1736613

CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCG                                                                                               >  SRR4302533.6256766/1‑94 (MQ=60)

 GCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGG                                                                                              >  SRR4302533.3813258/1‑94 (MQ=60)

 GCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGAGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGG                                                                                              >  SRR4302533.459035/1‑94 (MQ=60)

   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                            <  SRR4302533.759083/94‑1 (MQ=60)

   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                            >  SRR4302533.2347438/1‑94 (MQ=60)

   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                            >  SRR4302533.1657374/1‑94 (MQ=60)

   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                            >  SRR4302533.5488533/1‑94 (MQ=60)

     GAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTG                                                                                          >  SRR4302533.772401/1‑94 (MQ=60)

     GAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTG                                                                                          >  SRR4302533.4087337/1‑94 (MQ=60)

     GAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTG                                                                                          <  SRR4302533.4164769/94‑1 (MQ=60)

      AACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGA                                                                                         >  SRR4302533.6119761/1‑94 (MQ=60)

       ACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGAC                                                                                        >  SRR4302533.3000541/1‑94 (MQ=60)

        CGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACT                                                                                       >  SRR4302533.2188471/1‑94 (MQ=60)

                 GGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTG                                                                              >  SRR4302533.1760741/1‑94 (MQ=60)

                 GGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTG                                                                              >  SRR4302533.616954/1‑94 (MQ=60)

                   CGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAA                                                                            <  SRR4302533.6026829/94‑1 (MQ=60)

                    GCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAT                                                                           >  SRR4302533.5486274/1‑94 (MQ=60)

                    GCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAT                                                                           <  SRR4302533.1414323/94‑1 (MQ=60)

                    GCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAT                                                                           <  SRR4302533.5832640/94‑1 (MQ=60)

                    GCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAAT                                                                           >  SRR4302533.1260881/1‑94 (MQ=60)

                     CAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATC                                                                          <  SRR4302533.6015810/94‑1 (MQ=60)

                     CAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATC                                                                          <  SRR4302533.4381348/94‑1 (MQ=60)

                      AGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCC                                                                         <  SRR4302533.4320534/94‑1 (MQ=60)

                      AGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCC                                                                         >  SRR4302533.5182606/1‑94 (MQ=60)

                       GGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCG                                                                        <  SRR4302533.410148/94‑1 (MQ=60)

                       GGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCG                                                                        >  SRR4302533.2080078/1‑94 (MQ=60)

                       GGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCG                                                                        >  SRR4302533.5598768/1‑94 (MQ=60)

                         CCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGT                                                                      <  SRR4302533.4213452/94‑1 (MQ=60)

                         CCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGGGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGT                                                                      <  SRR4302533.1028376/94‑1 (MQ=60)

                          CGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTT                                                                     >  SRR4302533.2020593/1‑94 (MQ=60)

                          CGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTT                                                                     >  SRR4302533.1182115/1‑94 (MQ=60)

                          CGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTT                                                                     <  SRR4302533.3869957/94‑1 (MQ=60)

                          CGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTT                                                                     >  SRR4302533.5330695/1‑94 (MQ=60)

                           GCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTA                                                                    >  SRR4302533.1809836/1‑94 (MQ=60)

                           GCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTA                                                                    >  SRR4302533.3630074/1‑94 (MQ=60)

                            CCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                   >  SRR4302533.5354159/1‑94 (MQ=60)

                            CCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                   <  SRR4302533.441722/94‑1 (MQ=60)

                            CCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                   >  SRR4302533.4852208/1‑94 (MQ=60)

                            CCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                   <  SRR4302533.2886657/94‑1 (MQ=60)

                             CTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATC                                                                  >  SRR4302533.3141762/1‑94 (MQ=60)

                             CTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATC                                                                  >  SRR4302533.4684523/1‑94 (MQ=60)

                             CTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATC                                                                  >  SRR4302533.4619826/1‑94 (MQ=60)

                                GCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGGGACTTTGAACCTGAATCCGGTTATCGCG                                                               >  SRR4302533.787481/1‑94 (MQ=60)

                                  CGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCC                                                             >  SRR4302533.6190614/1‑94 (MQ=60)

                                   GGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCA                                                            >  SRR4302533.368631/1‑94 (MQ=60)

                                   GGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCA                                                            >  SRR4302533.129641/1‑94 (MQ=60)

                                     TTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATG                                                          >  SRR4302533.5808593/1‑94 (MQ=60)

                                       TTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCA                                                        <  SRR4302533.4402089/94‑1 (MQ=60)

                                       TTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCA                                                        <  SRR4302533.6229030/94‑1 (MQ=60)

                                        TATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAG                                                       >  SRR4302533.5446125/1‑94 (MQ=60)

                                        TATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTAGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAG                                                       <  SRR4302533.6336142/94‑1 (MQ=60)

                                         ATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGC                                                      >  SRR4302533.26454/1‑94 (MQ=60)

                                          TGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCA                                                     >  SRR4302533.6076042/1‑94 (MQ=60)

                                          TGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCA                                                     >  SRR4302533.5846463/1‑94 (MQ=60)

                                           GAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAA                                                    <  SRR4302533.4002564/94‑1 (MQ=60)

                                            AAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAA                                                   <  SRR4302533.2823917/94‑1 (MQ=60)

                                            AAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAA                                                   >  SRR4302533.526548/1‑94 (MQ=60)

                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAT                                                  <  SRR4302533.4356114/94‑1 (MQ=60)

                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAT                                                  <  SRR4302533.2522916/94‑1 (MQ=60)

                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAC                                                  >  SRR4302533.483535/1‑94 (MQ=60)

                                              GGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATC                                                 >  SRR4302533.1750618/1‑94 (MQ=60)

                                                CGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCT                                               >  SRR4302533.2299023/1‑94 (MQ=60)

                                                 GATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                              >  SRR4302533.6057435/1‑94 (MQ=60)

                                                  ATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGACCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGT                                             <  SRR4302533.208828/94‑1 (MQ=60)

                                                   TGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTC                                            <  SRR4302533.4943604/94‑1 (MQ=60)

                                                    GGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCG                                           <  SRR4302533.5406232/94‑1 (MQ=60)

                                                    GGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCG                                           >  SRR4302533.1218326/1‑94 (MQ=60)

                                                    GGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCG                                           <  SRR4302533.6077505/94‑1 (MQ=60)

                                                        GAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGC                                       <  SRR4302533.3514341/94‑1 (MQ=60)

                                                        GAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGC                                       <  SRR4302533.3514340/94‑1 (MQ=60)

                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                      >  SRR4302533.3784977/1‑94 (MQ=60)

                                                            CGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCA                                   <  SRR4302533.388837/94‑1 (MQ=60)

                                                            CGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCA                                   >  SRR4302533.2389400/1‑94 (MQ=60)

                                                             GATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCGGCAAATCCTGTCGCAGCCGCAT                                  <  SRR4302533.5800217/94‑1 (MQ=60)

                                                             GATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAT                                  >  SRR4302533.2050861/1‑94 (MQ=60)

                                                              ATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATC                                 <  SRR4302533.156608/94‑1 (MQ=60)

                                                                 ATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCC                              <  SRR4302533.1291444/94‑1 (MQ=60)

                                                                   CAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCT                            >  SRR4302533.5302555/1‑94 (MQ=60)

                                                                   CAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCT                            <  SRR4302533.5800724/94‑1 (MQ=60)

                                                                   CAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCT                            >  SRR4302533.1832737/1‑94 (MQ=60)

                                                                     AGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAC                          >  SRR4302533.5274151/1‑94 (MQ=60)

                                                                      GGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACT                         <  SRR4302533.2655881/94‑1 (MQ=60)

                                                                      GGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACT                         <  SRR4302533.5686929/94‑1 (MQ=60)

                                                                       GTGCAGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCAGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCTCCCTACTG                        <  SRR4302533.1531279/94‑1 (MQ=60)

                                                                        TGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGC                       >  SRR4302533.4326976/1‑94 (MQ=60)

                                                                          CCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCG                     >  SRR4302533.2842266/1‑94 (MQ=60)

                                                                          CCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCG                     >  SRR4302533.290630/1‑94 (MQ=60)

                                                                           CGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGT                    >  SRR4302533.1354332/1‑94 (MQ=60)

                                                                           CGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGT                    >  SRR4302533.67095/1‑94 (MQ=60)

                                                                              AAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTT                 >  SRR4302533.6100566/1‑94 (MQ=60)

                                                                              AAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTT                 <  SRR4302533.5964447/94‑1 (MQ=60)

                                                                               AAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTC                >  SRR4302533.496104/1‑94 (MQ=60)

                                                                                AGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCT               <  SRR4302533.5297066/94‑1 (MQ=60)

                                                                                AGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCT               <  SRR4302533.6347649/94‑1 (MQ=60)

                                                                                AGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCT               >  SRR4302533.1694523/1‑94 (MQ=60)

                                                                                 GCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTG              <  SRR4302533.2577221/94‑1 (MQ=60)

                                                                                 GCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTG              <  SRR4302533.3135240/94‑1 (MQ=60)

                                                                                  CTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTTTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGT             <  SRR4302533.4010223/94‑1 (MQ=60)

                                                                                  CTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGT             <  SRR4302533.6332969/94‑1 (MQ=60)

                                                                                      ATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTG         <  SRR4302533.1164482/94‑1 (MQ=60)

                                                                                      ATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTG         <  SRR4302533.6117852/94‑1 (MQ=60)

                                                                                      ATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTAGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTG         <  SRR4302533.3351315/94‑1 (MQ=60)

                                                                                       TTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGG        <  SRR4302533.6150910/94‑1 (MQ=60)

                                                                                       TTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGG        >  SRR4302533.5395274/1‑94 (MQ=60)

                                                                                         GTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCG      <  SRR4302533.1466853/94‑1 (MQ=60)

                                                                                            CGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATA   <  SRR4302533.406818/94‑1 (MQ=60)

                                                                                            CGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATA   >  SRR4302533.2672073/1‑94 (MQ=60)

                                                                                             GGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCTGTCTTCTGTGGTGGCGATAT  >  SRR4302533.4228839/1‑94 (MQ=60)

CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATAT  >  NC_000913/1736427‑1736613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`