Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,750,491 T→C Y455C (TAC→TGC)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,750,4910TC100.0% 697.2 / NA 215Y455C (TAC→TGC) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (108/107);  total (108/107)

Only 100 of 219 total aligned reads displayed.

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580
                                                                                          |                                                                                         
 cAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAct                                                                                        <  1:1888294/93‑1 (MQ=255)
 cAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAct                                                                                        <  1:2279685/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActc                                                                                       >  2:5252481/1‑93 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActc                                                                                       >  1:3731722/1‑93 (MQ=255)
    tGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCtt                                                                                     >  1:862459/1‑93 (MQ=255)
       aaCCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCAc                                                                                  <  2:4432614/93‑1 (MQ=255)
         ccGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCAccc                                                                                <  1:1413352/93‑1 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             <  2:5064617/93‑1 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             <  2:1671416/93‑1 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  2:3319680/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             <  2:349481/93‑1 (MQ=255)
             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                            >  1:1221028/1‑93 (MQ=255)
             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                            <  2:5491889/93‑1 (MQ=255)
              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                           <  2:1178851/93‑1 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                          >  1:542615/1‑93 (MQ=255)
                gcgcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTcc                                                                         <  2:399148/93‑1 (MQ=255)
                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                       >  2:2382024/1‑93 (MQ=255)
                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                       >  1:2366704/1‑93 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      <  2:3188140/93‑1 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      <  1:3248208/93‑1 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      >  2:2339583/1‑93 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      >  1:105174/1‑93 (MQ=255)
                    tGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCg                                                                     >  1:5990038/1‑93 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    >  2:2923519/1‑93 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    >  2:4293455/1‑93 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    <  2:6057463/93‑1 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 >  1:4029717/1‑93 (MQ=255)
                         cTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTg                                                                >  1:2624903/1‑93 (MQ=255)
                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                              >  2:1061758/1‑93 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            >  2:1733305/1‑93 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            >  1:5919043/1‑93 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            <  1:5840404/93‑1 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            >  1:5806721/1‑93 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            >  2:1576712/1‑93 (MQ=255)
                              ttGGCATGGTGAACCGGATCGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATg                                                           >  2:6155129/1‑93 (MQ=255)
                              ttGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATg                                                           <  1:5057088/93‑1 (MQ=255)
                              ttGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATg                                                           >  1:2743218/1‑93 (MQ=255)
                                ggCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGc                                                         <  2:2389952/93‑1 (MQ=255)
                                  cATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCat                                                       <  2:5825917/93‑1 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                      <  2:62178/93‑1 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                      <  1:2883368/93‑1 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                     >  1:2799833/1‑93 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                     <  2:3649024/93‑1 (MQ=255)
                                     ggTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATg                                                    >  1:4756439/1‑93 (MQ=255)
                                      gTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGa                                                   <  2:3876182/93‑1 (MQ=255)
                                      gTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGa                                                   <  2:2852828/93‑1 (MQ=255)
                                        gAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAc                                                 <  1:5275450/93‑1 (MQ=255)
                                        gAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAc                                                 >  1:1595059/1‑93 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                               >  1:3156729/1‑93 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             >  1:3340507/1‑93 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  1:5657980/93‑1 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                           >  1:5582645/1‑93 (MQ=255)
                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                          <  2:2281136/93‑1 (MQ=255)
                                                 aGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                        <  2:1837631/93‑1 (MQ=255)
                                                  gcgaaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAg                                       <  1:841660/90‑1 (MQ=255)
                                                  gCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAg                                       <  1:3858035/93‑1 (MQ=255)
                                                   ccAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAgg                                      <  1:6010622/93‑1 (MQ=255)
                                                   ccAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAgg                                      <  1:4270802/93‑1 (MQ=255)
                                                   ccAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAgg                                      >  2:2595026/1‑93 (MQ=255)
                                                    cAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGt                                     <  2:4021460/93‑1 (MQ=255)
                                                    cAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGt                                     <  1:1649033/93‑1 (MQ=255)
                                                    cAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGt                                     >  2:1130591/1‑93 (MQ=255)
                                                     aaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                    >  2:457667/1‑93 (MQ=255)
                                                       aCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAc                                  >  2:475518/1‑93 (MQ=255)
                                                       aCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAc                                  >  2:3235333/1‑93 (MQ=255)
                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                                <  2:129549/93‑1 (MQ=255)
                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                                <  2:4074845/93‑1 (MQ=255)
                                                          gCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCa                               <  1:5210357/93‑1 (MQ=255)
                                                           cGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAg                              <  2:2768434/93‑1 (MQ=255)
                                                            gACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGc                             >  1:306235/1‑93 (MQ=255)
                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCt                            >  1:2542877/1‑93 (MQ=255)
                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCt                            >  2:4123107/1‑93 (MQ=255)
                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                           <  2:4521460/93‑1 (MQ=255)
                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                           >  2:2217897/1‑93 (MQ=255)
                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                           <  2:3791864/93‑1 (MQ=255)
                                                                   gTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCca                      >  2:5398982/1‑93 (MQ=255)
                                                                   gTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCca                      <  1:4304820/93‑1 (MQ=255)
                                                                     ttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATTAGCCAGGTGACTCAGCTCACCcaca                    >  2:5916218/1‑93 (MQ=255)
                                                                     ttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcaca                    <  1:4732144/93‑1 (MQ=255)
                                                                     ttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcaca                    <  2:3094843/93‑1 (MQ=255)
                                                                       aTTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACg                  >  1:969924/1‑93 (MQ=255)
                                                                           ttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATgc              <  1:2168339/93‑1 (MQ=255)
                                                                              gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  2:3740154/1‑93 (MQ=255)
                                                                               cgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCa          <  1:5449735/93‑1 (MQ=255)
                                                                                gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  2:1870259/1‑93 (MQ=255)
                                                                                gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  2:1581166/1‑93 (MQ=255)
                                                                                gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  2:1947754/1‑93 (MQ=255)
                                                                                 gaGCCCCAGCGCTCTTCAGCCAAATTCCAGCGTTCTGGGATGGCAGATGAACCATCAGCCAGGTGACTGAGCCCCCCCACACGATGCGCCAGa        <  2:5480315/92‑1 (MQ=255)
                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       <  2:5086998/93‑1 (MQ=255)
                                                                                   gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATTCGCCAGACg      <  1:6300670/93‑1 (MQ=255)
                                                                                   gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg      <  2:5229119/93‑1 (MQ=255)
                                                                                   gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg      >  1:1747034/1‑93 (MQ=255)
                                                                                    ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg     <  2:5182524/93‑1 (MQ=255)
                                                                                    ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg     <  1:4106403/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt    <  1:3692588/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt    <  2:6093877/93‑1 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   <  2:5757170/93‑1 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   >  1:4853319/1‑93 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   <  1:4422259/93‑1 (MQ=255)
                                                                                       cAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgtag  <  2:4891539/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750398‑1750584
                                                                                             |                                                                                             
TTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGC                                                                                               >  SRR4302533.53596/1‑94 (MQ=60)
 TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302533.3618052/94‑1 (MQ=60)
 TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302533.2752399/94‑1 (MQ=60)
 TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302533.4480665/94‑1 (MQ=60)
  AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                             <  SRR4302533.2825287/94‑1 (MQ=60)
    CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTC                                                                                           <  SRR4302533.1899831/94‑1 (MQ=60)
    CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTC                                                                                           >  SRR4302533.3754611/1‑94 (MQ=60)
    CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTC                                                                                           <  SRR4302533.2293802/94‑1 (MQ=60)
     AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          >  SRR4302533.1384466/1‑94 (MQ=60)
      ATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTT                                                                                         <  SRR4302533.5252807/94‑1 (MQ=60)
      ATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTT                                                                                         >  SRR4302533.867514/1‑94 (MQ=60)
       TGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTC                                                                                        >  SRR4302533.4971689/1‑94 (MQ=60)
       AGGAACCGTCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTC                                                                                        <  SRR4302533.2795648/94‑1 (MQ=60)
        GGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCA                                                                                       >  SRR4302533.3169319/1‑94 (MQ=60)
        GGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCA                                                                                       <  SRR4302533.5472082/94‑1 (MQ=60)
            CCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCA                                                                                   <  SRR4302533.1421899/94‑1 (MQ=60)
            CCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCA                                                                                   >  SRR4302533.5928004/1‑94 (MQ=60)
             CGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAA                                                                                  >  SRR4302533.3842726/1‑94 (MQ=60)
              GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                 >  SRR4302533.4297154/1‑94 (MQ=60)
               ACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAT                                                                                >  SRR4302533.1228354/1‑94 (MQ=60)
                CCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATT                                                                               <  SRR4302533.3119958/94‑1 (MQ=60)
                 CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302533.545836/1‑94 (MQ=60)
                 CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              <  SRR4302533.1704325/94‑1 (MQ=60)
                   GCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCA                                                                            <  SRR4302533.6033401/94‑1 (MQ=60)
                    CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAG                                                                           >  SRR4302533.2381341/1‑94 (MQ=60)
                    CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAG                                                                           >  SRR4302533.3884034/1‑94 (MQ=60)
                     GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGC                                                                          <  SRR4302533.4432891/94‑1 (MQ=60)
                     GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGC                                                                          >  SRR4302533.105828/1‑94 (MQ=60)
                      CTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCG                                                                         <  SRR4302533.881772/94‑1 (MQ=60)
                      CTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCG                                                                         <  SRR4302533.3268237/94‑1 (MQ=60)
                      CTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCG                                                                         >  SRR4302533.6026322/1‑94 (MQ=60)
                         ACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTC                                                                      <  SRR4302533.4141580/94‑1 (MQ=60)
                          CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     <  SRR4302533.4783194/94‑1 (MQ=60)
                          CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     <  SRR4302533.1570781/94‑1 (MQ=60)
                          CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     >  SRR4302533.4054246/1‑94 (MQ=60)
                           GCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                    >  SRR4302533.2641009/1‑94 (MQ=60)
                             TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  >  SRR4302533.585217/1‑94 (MQ=60)
                             TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  >  SRR4302533.2123709/1‑94 (MQ=60)
                               CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                                >  SRR4302533.5841785/1‑94 (MQ=60)
                               CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                                >  SRR4302533.5954818/1‑94 (MQ=60)
                                ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               <  SRR4302533.5875717/94‑1 (MQ=60)
                                ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               >  SRR4302533.2760039/1‑94 (MQ=60)
                                 TTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGG                                                              <  SRR4302533.5087652/94‑1 (MQ=60)
                                      ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302533.2817052/1‑94 (MQ=60)
                                      ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302533.5823509/1‑94 (MQ=60)
                                      ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         <  SRR4302533.2901103/94‑1 (MQ=60)
                                      ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302533.852307/1‑94 (MQ=60)
                                       TGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATG                                                        >  SRR4302533.4711484/1‑94 (MQ=60)
                                       TGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATG                                                        >  SRR4302533.4785302/1‑94 (MQ=60)
                                          TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     >  SRR4302533.1604712/1‑94 (MQ=60)
                                           GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCATTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    <  SRR4302533.5567609/94‑1 (MQ=60)
                                           GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    <  SRR4302533.5307080/94‑1 (MQ=60)
                                            AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCA                                                   >  SRR4302533.3176140/1‑94 (MQ=60)
                                              CCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATC                                                 >  SRR4302533.3361153/1‑94 (MQ=60)
                                               CGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCA                                                >  SRR4302533.5970275/1‑94 (MQ=60)
                                                GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               >  SRR4302533.5243896/1‑94 (MQ=60)
                                                GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302533.5692170/94‑1 (MQ=60)
                                                GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               >  SRR4302533.5616375/1‑94 (MQ=60)
                                                   TAGCCAAACGGCTACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302533.1143412/94‑1 (MQ=60)
                                                   TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            >  SRR4302533.2746974/1‑94 (MQ=60)
                                                   TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGCAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302533.5156398/94‑1 (MQ=60)
                                                    AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           <  SRR4302533.1890413/94‑1 (MQ=60)
                                                    AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           <  SRR4302533.577348/94‑1 (MQ=60)
                                                     GCGAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302533.846581/94‑1 (MQ=60)
                                                     GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302533.3881592/94‑1 (MQ=60)
                                                      CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         <  SRR4302533.4296508/94‑1 (MQ=60)
                                                      CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         <  SRR4302533.6047037/94‑1 (MQ=60)
                                                       CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        <  SRR4302533.1659025/94‑1 (MQ=60)
                                                            GGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCA                                   >  SRR4302533.3287959/1‑94 (MQ=60)
                                                             GCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAG                                  <  SRR4302533.5241741/94‑1 (MQ=60)
                                                              CGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGC                                 >  SRR4302533.308107/1‑94 (MQ=60)
                                                              CGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGC                                 >  SRR4302533.2517804/1‑94 (MQ=60)
                                                               GACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCT                                <  SRR4302533.3888549/94‑1 (MQ=60)
                                                               GACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCT                                >  SRR4302533.2558525/1‑94 (MQ=60)
                                                                 CCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCA                              >  SRR4302533.2592063/1‑94 (MQ=60)
                                                                 CCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCA                              >  SRR4302533.1785077/1‑94 (MQ=60)
                                                                   AGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACC                            >  SRR4302533.161812/1‑94 (MQ=60)
                                                                    GAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCGCCC                           >  SRR4302533.2994945/1‑94 (MQ=60)
                                                                     AGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGTCTCAGCTCACCCA                          <  SRR4302533.774853/94‑1 (MQ=60)
                                                                      GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         <  SRR4302533.4330720/94‑1 (MQ=60)
                                                                      GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         >  SRR4302533.780128/1‑94 (MQ=60)
                                                                        TTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       <  SRR4302533.4760832/94‑1 (MQ=60)
                                                                         TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302533.975614/1‑94 (MQ=60)
                                                                           TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302533.2268646/94‑1 (MQ=60)
                                                                           TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302533.4060987/94‑1 (MQ=60)
                                                                              TTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCG                 <  SRR4302533.5667004/94‑1 (MQ=60)
                                                                              TTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCG                 <  SRR4302533.2181676/94‑1 (MQ=60)
                                                                              TTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCG                 >  SRR4302533.2871908/1‑94 (MQ=60)
                                                                                 GCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCA              <  SRR4302533.3249007/94‑1 (MQ=60)
                                                                                  CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             <  SRR4302533.5482563/94‑1 (MQ=60)
                                                                                  CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             <  SRR4302533.6070930/94‑1 (MQ=60)
                                                                                  CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302533.3581858/1‑94 (MQ=60)
                                                                                     AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          >  SRR4302533.1757666/1‑94 (MQ=60)
                                                                                     AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          <  SRR4302533.3729991/94‑1 (MQ=60)
                                                                                     AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          >  SRR4302533.4601569/1‑94 (MQ=60)
                                                                                      GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATTCGCCAGACGG         <  SRR4302533.6338295/94‑1 (MQ=60)
                                                                                      GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG         <  SRR4302533.4863217/94‑1 (MQ=60)
                                                                                       CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        <  SRR4302533.4131375/94‑1 (MQ=60)
                                                                                        CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA       <  SRR4302533.3715274/94‑1 (MQ=60)
                                                                                        CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA       >  SRR4302533.4882731/1‑94 (MQ=60)
                                                                                         CCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG      <  SRR4302533.4448998/94‑1 (MQ=60)
                                                                                         CCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG      <  SRR4302533.2374146/94‑1 (MQ=60)
                                                                                          CAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGT     <  SRR4302533.2567969/94‑1 (MQ=60)
                                                                                          CAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGA     >  SRR4302533.5588466/1‑94 (MQ=60)
                                                                                            GCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA   <  SRR4302533.3023317/94‑1 (MQ=60)
                                                                                            GCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA   <  SRR4302533.5671318/94‑1 (MQ=60)
                                                                                             CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  SRR4302533.4270456/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750398‑1750584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑