Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,750,491 T→C Y455C (TAC→TGC)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,750,4910TC99.7% 945.1 / ‑5.0 293Y455C (TAC→TGC) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (134/157);  total (135/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 299 total aligned reads displayed.

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA  >  NC_000913/1750401‑1750579
                                                                                          |                                                                                        
aCAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAc                                                                                        <  2:8741267/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActc                                                                                      <  2:2813315/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCActc                                                                                      >  1:8399731/1‑93 (MQ=255)
     ggAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTc                                                                                   <  2:4094786/93‑1 (MQ=255)
      gAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCa                                                                                  <  2:9566669/93‑1 (MQ=255)
          cGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCa                                                                              <  2:1032125/93‑1 (MQ=255)
           gACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCAAAACGGCGACCAGAGATTATTTTTCGCGTAGGCCCAGCACTCTTCACCCaa                                                                             >  2:7056070/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                            <  1:1100416/93‑1 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                            >  1:1713608/1‑93 (MQ=255)
             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                           >  1:1587964/1‑93 (MQ=255)
             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                           <  2:3733063/93‑1 (MQ=255)
              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                          <  1:1194755/93‑1 (MQ=255)
              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                          <  1:3771105/93‑1 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  1:3381568/1‑93 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  2:6553734/1‑93 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  1:9277692/1‑93 (MQ=255)
                 cgcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCa                                                                       <  1:7063574/93‑1 (MQ=255)
                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                      <  2:5852922/93‑1 (MQ=255)
                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                      <  1:6106559/93‑1 (MQ=255)
                     gccGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATCCCAGCGt                                                                   <  2:6429264/91‑1 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                <  1:9555745/93‑1 (MQ=255)
                         cTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTg                                                               >  2:2465439/1‑93 (MQ=255)
                          ttCATTTGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  1:3453291/93‑1 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              >  1:6765652/1‑93 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              >  1:2599300/1‑93 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  1:2307179/93‑1 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  2:5163838/93‑1 (MQ=255)
                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                             >  2:1873686/1‑93 (MQ=255)
                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                             >  2:1337348/1‑93 (MQ=255)
                            cATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGa                                                            <  2:8320529/93‑1 (MQ=255)
                            cATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGa                                                            <  1:2704192/93‑1 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                           <  1:9106040/93‑1 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                           >  2:3607382/1‑93 (MQ=255)
                               tGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                                         <  1:4670789/93‑1 (MQ=255)
                                 gCATGGTGAACCGGATAGCGAAACGGCGACCAGAGTTTATTGTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCa                                                       <  1:7342676/93‑1 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                     <  1:7786545/93‑1 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    <  2:1464156/93‑1 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  1:2912715/1‑93 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  1:579570/1‑93 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  2:4936717/1‑93 (MQ=255)
                                     ggTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATg                                                   <  2:1711750/93‑1 (MQ=255)
                                       tGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGaa                                                 <  1:3060846/93‑1 (MQ=255)
                                       tGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGaa                                                 <  1:9761771/93‑1 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                              >  1:8274394/1‑93 (MQ=255)
                                           ccGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAt                                             <  2:3309218/93‑1 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                            >  2:7831401/1‑93 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                            >  2:8056072/1‑93 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           <  1:3177764/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           <  1:3931996/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           >  2:5689059/1‑93 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          >  2:1408275/1‑93 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          <  1:5604618/93‑1 (MQ=255)
                                              gATAGCCAAACGGAGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          <  2:6403330/93‑1 (MQ=255)
                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         >  1:220519/1‑93 (MQ=255)
                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         >  2:279507/1‑93 (MQ=255)
                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         <  1:8211820/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                        >  2:7695631/1‑93 (MQ=255)
                                                 aGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                       >  2:5739974/1‑93 (MQ=255)
                                                 aGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                       <  2:238929/93‑1 (MQ=255)
                                                     aaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                   >  2:7951739/1‑93 (MQ=255)
                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                  <  1:8846970/93‑1 (MQ=255)
                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                  >  1:854532/1‑93 (MQ=255)
                                                       aCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAc                                 >  2:4294036/1‑93 (MQ=255)
                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                               >  2:4441069/1‑93 (MQ=255)
                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                               <  1:1882653/93‑1 (MQ=255)
                                                            gACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGc                            >  1:5306012/1‑93 (MQ=255)
                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCt                           <  2:7530852/93‑1 (MQ=255)
                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAACATCAGCCAGGTGACTCAGCt                           >  2:8390542/1‑93 (MQ=255)
                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                          >  2:8136537/1‑93 (MQ=255)
                                                               cAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATCCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCa                         <  2:6821225/93‑1 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  2:2980726/1‑93 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  1:4799806/1‑93 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        <  1:4557251/93‑1 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  1:4155068/1‑93 (MQ=255)
                                                                  agTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAccc                      >  1:2430608/1‑93 (MQ=255)
                                                                  agTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAccc                      >  1:4995428/1‑93 (MQ=255)
                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                    >  1:6448563/1‑93 (MQ=255)
                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                    >  2:4721476/1‑93 (MQ=255)
                                                                      tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                  <  2:343684/93‑1 (MQ=255)
                                                                      tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                  >  2:6777009/1‑93 (MQ=255)
                                                                       aTTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACg                 >  1:1790351/1‑93 (MQ=255)
                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  1:9355283/93‑1 (MQ=255)
                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  1:3593367/93‑1 (MQ=255)
                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  2:3130021/93‑1 (MQ=255)
                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  2:3806917/93‑1 (MQ=255)
                                                                            tCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATgcg            <  2:3893258/93‑1 (MQ=255)
                                                                            tCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATgcg            <  2:2553515/93‑1 (MQ=255)
                                                                               cgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCa         <  2:5379728/93‑1 (MQ=255)
                                                                                 tAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGa       <  2:2214412/93‑1 (MQ=255)
                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      <  2:8284960/93‑1 (MQ=255)
                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      <  1:5701001/93‑1 (MQ=255)
                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      >  2:9199147/1‑93 (MQ=255)
                                                                                   gCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg     <  1:8742728/93‑1 (MQ=255)
                                                                                    ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg    <  1:7234323/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  2:174036/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   >  1:7634104/1‑93 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  1:4512347/93‑1 (MQ=255)
                                                                                     cccAGAACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGTACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCGCGACggg   >  2:5484935/1‑92 (MQ=255)
                                                                                     cacAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  1:4344098/91‑1 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta  <  2:6268199/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA  >  NC_000913/1750401‑1750579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750399‑1750584
                                                                                            |                                                                                             
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302540.6323199/94‑1 (MQ=60)
TAACAATGGAACCGACCTGCGCTGACGCGTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302540.6323200/94‑1 (MQ=60)
 AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGACCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                             <  SRR4302540.2758902/94‑1 (MQ=60)
 AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                             <  SRR4302540.6965451/94‑1 (MQ=60)
  ACAATGGACCCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACT                                                                                            <  SRR4302540.3533096/94‑1 (MQ=60)
   CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGGTTATTTTTCGCGTAGGCCCAGCACTC                                                                                           >  SRR4302540.458848/1‑94 (MQ=60)
   CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCACTC                                                                                           >  SRR4302540.8497211/1‑94 (MQ=60)
   CAATGGAACCGACCTGCGCTGACGCTTCATTGGCAT‑GTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          >  SRR4302540.8361493/1‑94 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          <  SRR4302540.4805169/94‑1 (MQ=60)
     ATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTT                                                                                         <  SRR4302540.7372586/94‑1 (MQ=60)
     ATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTT                                                                                         <  SRR4302540.4107254/94‑1 (MQ=60)
     ATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTT                                                                                         <  SRR4302540.6067249/94‑1 (MQ=60)
        GAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCAC                                                                                      <  SRR4302540.2450515/94‑1 (MQ=60)
         AACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACC                                                                                     <  SRR4302540.1806247/94‑1 (MQ=60)
         AACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACC                                                                                     <  SRR4302540.5818521/94‑1 (MQ=60)
          ACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCC                                                                                    >  SRR4302540.6584832/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCTAAA                                                                                 >  SRR4302540.3851265/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                 >  SRR4302540.1732121/1‑94 (MQ=60)
              ACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAT                                                                                <  SRR4302540.3450392/94‑1 (MQ=60)
              ACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAT                                                                                <  SRR4302540.1111749/94‑1 (MQ=60)
              ACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAT                                                                                >  SRR4302540.1605311/1‑94 (MQ=60)
               CCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATT                                                                               <  SRR4302540.4895612/94‑1 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302540.3418482/1‑94 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              <  SRR4302540.1207060/94‑1 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              <  SRR4302540.9476863/94‑1 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302540.6493728/1‑94 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              <  SRR4302540.3812247/94‑1 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302540.9388601/1‑94 (MQ=60)
                   CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAG                                                                           <  SRR4302540.7142135/94‑1 (MQ=60)
                    GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGC                                                                          <  SRR4302540.6174571/94‑1 (MQ=60)
                     CTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCG                                                                         >  SRR4302540.6044737/1‑94 (MQ=60)
                      TGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGGCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCATATTCCAGCGT                                                                        >  SRR4302540.7736125/1‑94 (MQ=60)
                      TGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGT                                                                        >  SRR4302540.8024733/1‑94 (MQ=60)
                      TGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGT                                                                        >  SRR4302540.773863/1‑94 (MQ=60)
                       GACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTT                                                                       <  SRR4302540.187693/94‑1 (MQ=60)
                        ACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTC                                                                      >  SRR4302540.2935804/1‑94 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     >  SRR4302540.8324431/1‑94 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     <  SRR4302540.9746138/94‑1 (MQ=60)
                          cgctccTTGGCATGGTGAACCGGATAGCCGAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                    <  SRR4302540.9668751/88‑1 (MQ=60)
                          GCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                    <  SRR4302540.9668750/94‑1 (MQ=60)
                           CTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGC                                                                   >  SRR4302540.6840423/1‑94 (MQ=60)
                           CTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGC                                                                   >  SRR4302540.2627328/1‑94 (MQ=60)
                            TTCATTTGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  <  SRR4302540.3490864/94‑1 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCCGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  >  SRR4302540.5697008/1‑94 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  <  SRR4302540.2332013/94‑1 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  <  SRR4302540.8922896/94‑1 (MQ=60)
                             TCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGA                                                                 >  SRR4302540.1677246/1‑94 (MQ=60)
                              CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                                <  SRR4302540.7537266/94‑1 (MQ=60)
                              CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                                <  SRR4302540.2733362/94‑1 (MQ=60)
                               ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCTCTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               <  SRR4302540.7734432/94‑1 (MQ=60)
                               ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               <  SRR4302540.5358064/94‑1 (MQ=60)
                               ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               <  SRR4302540.9215467/94‑1 (MQ=60)
                                TTGTCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGG                                                              <  SRR4302540.9431092/94‑1 (MQ=60)
                                TTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGG                                                              >  SRR4302540.2240048/1‑94 (MQ=60)
                                 TGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGC                                                             <  SRR4302540.4720777/94‑1 (MQ=60)
                                 TGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGC                                                             <  SRR4302540.4720779/94‑1 (MQ=60)
                                  GGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCA                                                            <  SRR4302540.2528547/94‑1 (MQ=60)
                                   GCATGGTGAACCGGATAGCGAAACGGCGACCAGAGTTTATTGTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCAT                                                           <  SRR4302540.7424602/94‑1 (MQ=60)
                                   GCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCAT                                                           <  SRR4302540.5508692/94‑1 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302540.2944563/1‑94 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302540.5736627/1‑94 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302540.585841/1‑94 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         <  SRR4302540.9450622/94‑1 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         >  SRR4302540.6681111/1‑94 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         <  SRR4302540.7877564/94‑1 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATAT                                                         <  SRR4302540.9358693/94‑1 (MQ=60)
                                       GGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGA                                                       <  SRR4302540.8592214/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302540.9876268/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302540.3050339/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302540.3094426/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302540.1227857/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302540.1187407/94‑1 (MQ=60)
                                          GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    <  SRR4302540.8689505/94‑1 (MQ=60)
                                           AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCA                                                   >  SRR4302540.9869663/1‑94 (MQ=60)
                                           AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCA                                                   >  SRR4302540.8370514/1‑94 (MQ=60)
                                           AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCACGTAGCCCCAGCACTCTTCAACCAAATTCCAGCATTCTGCTATGGCATAcagccca                                                   >  SRR4302540.8370513/1‑87 (MQ=60)
                                            ACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAT                                                  <  SRR4302540.4345224/94‑1 (MQ=60)
                                            ACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAT                                                  >  SRR4302540.7278624/1‑94 (MQ=60)
                                             CCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATC                                                 >  SRR4302540.5600593/1‑94 (MQ=60)
                                             ACGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATC                                                 <  SRR4302540.2281009/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302540.4724321/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302540.3212303/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302540.3974505/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTGTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302540.1786768/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTGATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302540.3520315/94‑1 (MQ=60)
                                                GATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGC                                              <  SRR4302540.5666284/94‑1 (MQ=60)
                                                GATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGC                                              >  SRR4302540.1686025/1‑94 (MQ=60)
                                                GATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGC                                              >  SRR4302540.222662/1‑94 (MQ=60)
                                                 ATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCC                                             >  SRR4302540.3255539/1‑94 (MQ=60)
                                                 ATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCC                                             <  SRR4302540.8307269/94‑1 (MQ=60)
                                                 ATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCC                                             >  SRR4302540.4598137/1‑94 (MQ=60)
                                                  TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302540.8801094/94‑1 (MQ=60)
                                                  TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            >  SRR4302540.6518911/1‑94 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           <  SRR4302540.2690390/94‑1 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           >  SRR4302540.3239306/1‑94 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302540.3768014/94‑1 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302540.5320504/94‑1 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302540.7139529/94‑1 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302540.6976556/94‑1 (MQ=60)
                                                     CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         >  SRR4302540.9483406/1‑94 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCATGTG                                        >  SRR4302540.2940373/1‑94 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        >  SRR4302540.2677575/1‑94 (MQ=60)
                                                       AAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGA                                       >  SRR4302540.863604/1‑94 (MQ=60)
                                                        AGCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTGCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                      <  SRR4302540.8949316/94‑1 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                      <  SRR4302540.8949317/94‑1 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAACATCAGACAGGTGAC                                      >  SRR4302540.1901172/1‑94 (MQ=60)
                                                          CGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTC                                    <  SRR4302540.8405767/94‑1 (MQ=60)
                                                          AGGCGACCAGAGTGTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTC                                    <  SRR4302540.8405768/94‑1 (MQ=60)
                                                           GGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCA                                   <  SRR4302540.2516255/94‑1 (MQ=60)
                                                           GGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCA                                   >  SRR4302540.3431129/1‑94 (MQ=60)
                                                           GGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCA                                   <  SRR4302540.1903275/94‑1 (MQ=60)
                                                             CGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGC                                 >  SRR4302540.5364074/1‑94 (MQ=60)
                                                              GACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCT                                >  SRR4302540.3882114/1‑94 (MQ=60)
                                                               ACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTC                               >  SRR4302540.401170/1‑94 (MQ=60)
                                                               ACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTC                               >  SRR4302540.9147894/1‑94 (MQ=60)
                                                                CCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCA                              <  SRR4302540.9123012/94‑1 (MQ=60)
                                                                CCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCA                              <  SRR4302540.9298079/94‑1 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302540.4851173/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302540.4200080/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302540.4404542/1‑94 (MQ=60)
                                                                  AGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCCAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACC                            >  SRR4302540.4991799/1‑94 (MQ=60)
                                                                  AGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACC                            <  SRR4302540.4606514/94‑1 (MQ=60)
                                                                   GAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCC                           >  SRR4302540.5049504/1‑94 (MQ=60)
                                                                   GAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCC                           >  SRR4302540.2456797/1‑94 (MQ=60)
                                                                   GAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCC                           <  SRR4302540.3202219/94‑1 (MQ=60)
                                                                     GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         >  SRR4302540.6520345/1‑94 (MQ=60)
                                                                     GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         >  SRR4302540.4651685/1‑94 (MQ=60)
                                                                       TTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       <  SRR4302540.8306637/94‑1 (MQ=60)
                                                                       TTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       <  SRR4302540.3172346/94‑1 (MQ=60)
                                                                        TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302540.1809812/1‑94 (MQ=60)
                                                                        TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302540.2344991/1‑94 (MQ=60)
                                                                        TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302540.4264714/1‑94 (MQ=60)
                                                                        TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302540.8634990/1‑94 (MQ=60)
                                                                        TATTTTTCCCGTAGCCCCAGCACTCTTCCCCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      <  SRR4302540.3062748/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302540.754139/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302540.3764793/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302540.3632619/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302540.9466707/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    >  SRR4302540.275629/1‑94 (MQ=60)
                                                                           TTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATG                   >  SRR4302540.7319170/1‑94 (MQ=60)
                                                                              TCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGC                <  SRR4302540.2201045/94‑1 (MQ=60)
                                                                                 CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302540.1296223/1‑94 (MQ=60)
                                                                                 CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302540.7254977/1‑94 (MQ=60)
                                                                                  GTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGA            >  SRR4302540.10077487/1‑94 (MQ=60)
                                                                                   TAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC           >  SRR4302540.10037483/1‑94 (MQ=60)
                                                                                   TAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC           <  SRR4302540.1908097/94‑1 (MQ=60)
                                                                                    AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          <  SRR4302540.5763770/94‑1 (MQ=60)
                                                                                     GCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG         <  SRR4302540.8844209/94‑1 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG         <  SRR4302540.5822503/94‑1 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        >  SRR4302540.7719987/1‑94 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        <  SRR4302540.7315011/94‑1 (MQ=60)
                                                                                       CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA       <  SRR4302540.4391382/94‑1 (MQ=60)
                                                                                       CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA       <  SRR4302540.4561237/94‑1 (MQ=60)
                                                                                       CACAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA       <  SRR4302540.4391381/94‑1 (MQ=60)
                                                                                         CAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGT     >  SRR4302540.10137711/1‑94 (MQ=60)
                                                                                         CAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGA     >  SRR4302540.6918581/1‑94 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302540.6090575/1‑94 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302540.3885075/1‑94 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302540.4992569/1‑94 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302540.2057561/1‑94 (MQ=60)
                                                                                           GCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA   <  SRR4302540.7753059/94‑1 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  <  SRR4302540.173265/94‑1 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  <  SRR4302540.2171814/94‑1 (MQ=60)
                                                                                            |                                                                                             
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750399‑1750584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑