Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,749	A→G	I10V (ATC→GTC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,749	0	A	G	100.0%	759.8 / NA	234	I10V (ATC→GTC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (128/106); total (128/106)

Only 100 of 236 total aligned reads displayed.

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTc                                                                                         <  2:4921603/93‑1 (MQ=255)

  cGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCgg                                                                                       >  1:2219677/1‑93 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     <  1:6185917/93‑1 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     >  2:4596713/1‑93 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     <  1:3635658/93‑1 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     <  2:4704775/93‑1 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  1:6036963/93‑1 (MQ=255)

       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  >  1:2777471/1‑93 (MQ=255)

         gcgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGaaa                                                                                <  1:2084168/93‑1 (MQ=255)

         gcgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGaaa                                                                                >  2:3358646/1‑93 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  2:5919249/93‑1 (MQ=255)

             ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCg                                                                            >  2:1497700/1‑93 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           >  2:3260518/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:2339122/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:1030558/93‑1 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         <  1:4287640/93‑1 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         >  2:828537/1‑93 (MQ=255)

                 aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATc                                                                        <  1:1123621/93‑1 (MQ=255)

                 aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATc                                                                        <  2:2131001/93‑1 (MQ=255)

                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:4293781/93‑1 (MQ=255)

                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:1890583/93‑1 (MQ=255)

                      aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAag                                                                   <  2:2499393/93‑1 (MQ=255)

                       aGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAaga                                                                  <  1:604827/93‑1 (MQ=255)

                       aGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAaga                                                                  <  2:5009268/93‑1 (MQ=255)

                        gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAagag                                                                 >  2:3428629/1‑93 (MQ=255)

                        gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAagag                                                                 >  1:4661245/1‑93 (MQ=255)

                          ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAt                                                               <  2:5516832/93‑1 (MQ=255)

                          ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAt                                                               >  2:517888/1‑93 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           <  1:5992764/93‑1 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           >  1:4924982/1‑93 (MQ=255)

                               ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                                          >  1:4722253/1‑93 (MQ=255)

                                 aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCt                                                        >  1:5339279/1‑93 (MQ=255)

                                 aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCt                                                        >  1:4345348/1‑93 (MQ=255)

                                  tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTa                                                       <  2:5215830/93‑1 (MQ=255)

                                    cTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaa                                                     <  2:4673439/93‑1 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    >  2:2294023/1‑93 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  2:4098990/93‑1 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  1:768260/93‑1 (MQ=255)

                                      tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                   >  1:5792581/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  1:780249/1‑93 (MQ=255)

                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGc                                                 >  1:4140445/1‑93 (MQ=255)

                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGc                                                 >  1:947529/1‑93 (MQ=255)

                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                <  1:5019696/93‑1 (MQ=255)

                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                <  1:1400042/93‑1 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               >  2:5380479/1‑93 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               >  2:3786600/1‑93 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               <  1:6259770/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  2:2810423/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            <  2:4572053/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            >  2:3654388/1‑93 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            <  2:3552695/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            <  2:6004998/93‑1 (MQ=255)

                                              tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                                           >  2:5565037/1‑93 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACgat                                         >  2:6094966/1‑91 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  2:1124386/93‑1 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         >  1:3818391/1‑93 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  2:2323688/93‑1 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  1:5909339/93‑1 (MQ=255)

                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  2:3145879/93‑1 (MQ=255)

                                                 agacgaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  1:2352882/93‑1 (MQ=255)

                                                     taagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                    >  2:4516548/1‑93 (MQ=255)

                                                     taagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                    >  1:4311499/1‑93 (MQ=255)

                                                      aagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                                   >  1:1178941/1‑93 (MQ=255)

                                                        gactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGaa                                 >  1:2476906/1‑93 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                >  1:4195597/1‑93 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                >  1:6124229/1‑93 (MQ=255)

                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               >  1:3262116/1‑93 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  2:1604065/1‑93 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  2:1950181/1‑93 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             <  1:1802898/93‑1 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             <  1:2156269/93‑1 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  1:5499108/1‑93 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  2:1309391/1‑93 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  1:3853632/1‑93 (MQ=255)

                                                                 gAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCg                        <  2:1236696/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:3608157/93‑1 (MQ=255)

                                                                      aGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                   >  2:122329/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:162326/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:3706352/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  2:2661132/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:4765133/1‑93 (MQ=255)

                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAc                 >  2:5808805/1‑93 (MQ=255)

                                                                         ccAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACt                >  1:867950/1‑93 (MQ=255)

                                                                          cAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtt               >  2:4156438/1‑93 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  2:4002921/1‑93 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              <  2:3956930/93‑1 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  1:1942041/1‑93 (MQ=255)

                                                                              aTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctct           >  1:4500787/1‑93 (MQ=255)

                                                                              aTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctct           >  1:3730547/1‑93 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc          >  1:1267394/1‑93 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc          <  2:88572/93‑1 (MQ=255)

                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         <  1:5655681/93‑1 (MQ=255)

                                                                                 gTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt        <  2:175015/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       >  1:4824421/1‑93 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  1:3345674/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGGTATGCGTCTGAACTTCTCTCATg       >  2:4858922/1‑93 (MQ=255)

                                                                                     gCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTg    >  1:1736213/1‑93 (MQ=255)

                                                                                      cACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGa   <  1:2430600/93‑1 (MQ=255)

                                                                                       aCCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAc  <  2:2957357/93‑1 (MQ=255)

                                                                                       aCCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAc  >  1:312387/1‑93 (MQ=255)

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753657‑1753842

TTTCCGGCAGTGCGCCCAGATAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGT                                                                                              <  SRR4302533.5340060/94‑1 (MQ=60)

 TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTC                                                                                             <  SRR4302533.5899251/94‑1 (MQ=60)

 TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTC                                                                                             <  SRR4302533.5372192/94‑1 (MQ=60)

 TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTC                                                                                             <  SRR4302533.6036433/94‑1 (MQ=60)

   CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           <  SRR4302533.4324762/94‑1 (MQ=60)

   CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           >  SRR4302533.2233380/1‑94 (MQ=60)

      GCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACC                                                                                        <  SRR4302533.8694/94‑1 (MQ=60)

      GCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACC                                                                                        <  SRR4302533.6223050/94‑1 (MQ=60)

      GCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACC                                                                                        <  SRR4302533.3658014/94‑1 (MQ=60)

       CAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCG                                                                                       <  SRR4302533.6073545/94‑1 (MQ=60)

        AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302533.783654/1‑94 (MQ=60)

        AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302533.62177/1‑94 (MQ=60)

        AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302533.2794534/1‑94 (MQ=60)

           GCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAA                                                                                   <  SRR4302533.693828/94‑1 (MQ=60)

           GCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAA                                                                                   <  SRR4302533.2097000/94‑1 (MQ=60)

              CCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATGGTTTGCACCGTCGGACCGAAAACCG                                                                                <  SRR4302533.484647/94‑1 (MQ=60)

               CCAGAAAGCAAGTTTCTCCCGTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACAGA                                                                               >  SRR4302533.1810721/1‑94 (MQ=60)

               CCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGA                                                                               >  SRR4302533.2334830/1‑94 (MQ=60)

                CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAACATTGTTTGCACCGTCGGACCGAAAACCGCA                                                                              >  SRR4302533.2377314/1‑94 (MQ=60)

                CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              >  SRR4302533.400241/1‑94 (MQ=60)

                 AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302533.2353606/94‑1 (MQ=60)

                 AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             >  SRR4302533.5465800/1‑94 (MQ=60)

                 AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302533.1036620/94‑1 (MQ=60)

                  GAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATC                                                                            <  SRR4302533.4313421/94‑1 (MQ=60)

                   AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           >  SRR4302533.1734091/1‑94 (MQ=60)

                   AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           <  SRR4302533.1130287/94‑1 (MQ=60)

                    AAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTG                                                                          >  SRR4302533.4046020/1‑94 (MQ=60)

                     AGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGA                                                                         >  SRR4302533.2209221/1‑94 (MQ=60)

                      GCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAA                                                                        <  SRR4302533.3219945/94‑1 (MQ=60)

                       CAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAG                                                                       >  SRR4302533.4260182/1‑94 (MQ=60)

                         AGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     >  SRR4302533.5759224/1‑94 (MQ=60)

                         AGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     >  SRR4302533.4689492/1‑94 (MQ=60)

                         AGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     <  SRR4302533.608378/94‑1 (MQ=60)

                           TTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAT                                                                   <  SRR4302533.4799096/94‑1 (MQ=60)

                            TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  <  SRR4302533.6023632/94‑1 (MQ=60)

                            TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  <  SRR4302533.5089820/94‑1 (MQ=60)

                              CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTT                                                                <  SRR4302533.4862170/94‑1 (MQ=60)

                              CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTT                                                                >  SRR4302533.2166681/1‑94 (MQ=60)

                               TCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTA                                                               <  SRR4302533.5624011/94‑1 (MQ=60)

                               TCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTA                                                               <  SRR4302533.4165962/94‑1 (MQ=60)

                               TCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTA                                                               >  SRR4302533.4954813/1‑94 (MQ=60)

                                CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              >  SRR4302533.4750884/1‑94 (MQ=60)

                                CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              <  SRR4302533.6029065/94‑1 (MQ=60)

                                CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              <  SRR4302533.5988029/94‑1 (MQ=60)

                                 CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302533.1280412/1‑94 (MQ=60)

                                  CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            >  SRR4302533.5371296/1‑94 (MQ=60)

                                  CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            >  SRR4302533.3067041/1‑94 (MQ=60)

                                  CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            >  SRR4302533.4371535/1‑94 (MQ=60)

                                   ATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTA                                                           >  SRR4302533.374158/1‑94 (MQ=60)

                                   ATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTA                                                           >  SRR4302533.2311682/1‑94 (MQ=60)

                                     CCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAA                                                         >  SRR4302533.145273/1‑94 (MQ=60)

                                      CTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGGTGTTAGCTAAAA                                                        >  SRR4302533.2668278/1‑94 (MQ=60)

                                       TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       >  SRR4302533.5827574/1‑94 (MQ=60)

                                       TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       <  SRR4302533.772790/94‑1 (MQ=60)

                                        TCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATG                                                      >  SRR4302533.784847/1‑94 (MQ=60)

                                         CTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGC                                                     >  SRR4302533.4165555/1‑94 (MQ=60)

                                         CTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGC                                                     >  SRR4302533.953100/1‑94 (MQ=60)

                                          TCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCT                                                    <  SRR4302533.1214986/94‑1 (MQ=60)

                                           CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   >  SRR4302533.2018949/1‑94 (MQ=60)

                                           CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302533.5050098/94‑1 (MQ=60)

                                           CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302533.1408481/94‑1 (MQ=60)

                                            AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  >  SRR4302533.6081082/1‑94 (MQ=60)

                                            AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  <  SRR4302533.6297197/94‑1 (MQ=60)

                                             ACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGA                                                 <  SRR4302533.2425686/94‑1 (MQ=60)

                                              CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302533.941992/94‑1 (MQ=60)

                                              CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302533.5016998/94‑1 (MQ=60)

                                              CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                >  SRR4302533.3646636/1‑94 (MQ=60)

                                               TTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACG                                               >  SRR4302533.2356225/1‑94 (MQ=60)

                                                 AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCT                                             >  SRR4302533.3841739/1‑94 (MQ=60)

                                                  AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTG                                            <  SRR4302533.5945037/94‑1 (MQ=60)

                                                   AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           >  SRR4302533.3798822/1‑94 (MQ=60)

                                                   AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           >  SRR4302533.6112477/1‑94 (MQ=60)

                                                   AGACGAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           <  SRR4302533.2367435/94‑1 (MQ=60)

                                                    GACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGC                                          <  SRR4302533.4394029/94‑1 (MQ=60)

                                                      CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        >  SRR4302533.4337454/1‑94 (MQ=60)

                                                      CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        >  SRR4302533.384538/1‑94 (MQ=60)

                                                      CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        >  SRR4302533.1963303/1‑94 (MQ=60)

                                                       TAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATG                                       >  SRR4302533.1186020/1‑94 (MQ=60)

                                                       TAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATG                                       >  SRR4302533.505875/1‑94 (MQ=60)

                                                         AGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAA                                     >  SRR4302533.1268244/1‑94 (MQ=60)

                                                         AGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAA                                     >  SRR4302533.2492181/1‑94 (MQ=60)

                                                          GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    <  SRR4302533.5831467/94‑1 (MQ=60)

                                                          GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    >  SRR4302533.6161145/1‑94 (MQ=60)

                                                          GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    >  SRR4302533.4220994/1‑94 (MQ=60)

                                                           ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   <  SRR4302533.4625740/94‑1 (MQ=60)

                                                           ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   >  SRR4302533.3282241/1‑94 (MQ=60)

                                                            CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  <  SRR4302533.5555491/94‑1 (MQ=60)

                                                              GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                <  SRR4302533.1813874/94‑1 (MQ=60)

                                                              GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                <  SRR4302533.2169545/94‑1 (MQ=60)

                                                              GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                >  SRR4302533.4326600/1‑94 (MQ=60)

                                                              GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                >  SRR4302533.6071484/1‑94 (MQ=60)

                                                              GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGCACGCTGGCATCAACGGTA                                >  SRR4302533.3804717/1‑94 (MQ=60)

                                                               TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               >  SRR4302533.1739528/1‑94 (MQ=60)

                                                                CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              <  SRR4302533.845669/94‑1 (MQ=60)

                                                                CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              >  SRR4302533.3331527/1‑94 (MQ=60)

                                                                 ATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGC                             <  SRR4302533.3092346/94‑1 (MQ=60)

                                                                 ATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGC                             >  SRR4302533.5532278/1‑94 (MQ=60)

                                                                 ATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGC                             >  SRR4302533.3877171/1‑94 (MQ=60)

                                                                   GAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGT                           <  SRR4302533.2490909/94‑1 (MQ=60)

                                                                   GAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGT                           >  SRR4302533.1153436/1‑94 (MQ=60)

                                                                    AAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTC                          >  SRR4302533.3424117/1‑94 (MQ=60)

                                                                     AAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT                         <  SRR4302533.1070090/94‑1 (MQ=60)

                                                                        AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302533.4794030/1‑94 (MQ=60)

                                                                        AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      <  SRR4302533.4776777/94‑1 (MQ=60)

                                                                        AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302533.3729117/1‑94 (MQ=60)

                                                                        AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302533.163361/1‑94 (MQ=60)

                                                                        AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302533.6260206/1‑94 (MQ=60)

                                                                         GACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAC                     <  SRR4302533.1911075/94‑1 (MQ=60)

                                                                          ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACT                    >  SRR4302533.873037/1‑94 (MQ=60)

                                                                            CAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTC                  >  SRR4302533.1953885/1‑94 (MQ=60)

                                                                             AAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCT                 >  SRR4302533.65207/1‑94 (MQ=60)

                                                                              AAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTC                <  SRR4302533.4720065/94‑1 (MQ=60)

                                                                               AATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCT               >  SRR4302533.4528049/1‑94 (MQ=60)

                                                                               AATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCT               <  SRR4302533.4540682/94‑1 (MQ=60)

                                                                               AATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCT               >  SRR4302533.3753430/1‑94 (MQ=60)

                                                                               AATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCT               <  SRR4302533.3724120/94‑1 (MQ=60)

                                                                                ATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTC              >  SRR4302533.1274977/1‑94 (MQ=60)

                                                                                  TGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAT            <  SRR4302533.5689857/94‑1 (MQ=60)

                                                                                   GTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATG           >  SRR4302533.4853643/1‑94 (MQ=60)

                                                                                    TTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGG          <  SRR4302533.3366345/94‑1 (MQ=60)

                                                                                     TTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGT         >  SRR4302533.6117743/1‑94 (MQ=60)

                                                                                      TGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTG        >  SRR4302533.1746748/1‑94 (MQ=60)

                                                                                        CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      >  SRR4302533.4366299/1‑94 (MQ=60)

                                                                                        CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      <  SRR4302533.2445624/94‑1 (MQ=60)

                                                                                        CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      >  SRR4302533.314297/1‑94 (MQ=60)

                                                                                         ACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACT     >  SRR4302533.3995413/1‑94 (MQ=60)

                                                                                          CCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTA    <  SRR4302533.3809554/94‑1 (MQ=60)

                                                                                          CCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTA    >  SRR4302533.1886953/1‑94 (MQ=60)

                                                                                           CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   >  SRR4302533.5544851/1‑94 (MQ=60)

                                                                                           CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   <  SRR4302533.3858949/94‑1 (MQ=60)

                                                                                           CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   >  SRR4302533.862605/1‑94 (MQ=60)

                                                                                           CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   >  SRR4302533.3993307/1‑94 (MQ=60)

                                                                                           CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   <  SRR4302533.5147738/94‑1 (MQ=60)

                                                                                            GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  <  SRR4302533.716491/94‑1 (MQ=60)

                                                                                            GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  SRR4302533.279703/1‑94 (MQ=60)

TTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753657‑1753842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`