Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,749	A→G	I10V (ATC→GTC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,749	0	A	G	99.4%	1046.1 / ‑3.4	322	I10V (ATC→GTC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (158/162); total (159/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 329 total aligned reads displayed.

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTc                                                                                         <  1:7883409/93‑1 (MQ=255)

 ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCg                                                                                        <  1:1132905/93‑1 (MQ=255)

 ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCg                                                                                        <  1:8563374/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      <  2:9773316/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      <  2:5770876/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      >  1:1676969/1‑93 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  2:6427881/93‑1 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  2:3385397/93‑1 (MQ=255)

      aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCg                                                                                   <  2:8463102/93‑1 (MQ=255)

       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  <  2:4935959/93‑1 (MQ=255)

       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  >  2:87804/1‑93 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  2:3679013/93‑1 (MQ=255)

            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             <  2:4066365/93‑1 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           >  2:2716900/1‑93 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           <  1:8047303/93‑1 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           <  1:5375071/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGCCTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:3818974/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:5257610/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  1:52618/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  1:5041734/1‑93 (MQ=255)

                gAAAGCAATTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         <  2:9299811/93‑1 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         >  1:3168490/1‑93 (MQ=255)

                 aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATc                                                                        <  1:7482640/93‑1 (MQ=255)

                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:9106216/93‑1 (MQ=255)

                   aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTg                                                                      >  2:2916488/1‑93 (MQ=255)

                   aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTg                                                                      <  1:1856195/93‑1 (MQ=255)

                      aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAag                                                                   <  2:3510583/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  2:5555883/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  1:118613/93‑1 (MQ=255)

                           tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATg                                                              <  1:4348554/93‑1 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           <  2:3175543/93‑1 (MQ=255)

                              cccATCCTTCTCAACCTAAAGACTAAGACTGTCATGAAAAAGCCCAAAATTGTTTGCACCGCCGGACCGAAAACCTAATCTGAAGAGATGtca                                                           >  1:1410766/1‑91 (MQ=255)

                               ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                                          <  2:4548638/93‑1 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         >  1:636970/1‑93 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         <  1:3106936/93‑1 (MQ=255)

                                  tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGACAACCGAATCTCACGAGATGTTAGCTa                                                       >  1:4967431/1‑93 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      <  1:227680/93‑1 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      >  2:5181078/1‑93 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  1:9058346/93‑1 (MQ=255)

                                      tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                   >  1:5555520/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  2:6890621/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  2:1953144/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  1:4862953/1‑93 (MQ=255)

                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATgt                                                 >  2:2603480/1‑92 (MQ=255)

                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                <  1:2934538/93‑1 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               >  2:4821191/1‑93 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:2222417/1‑93 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:6608433/1‑93 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  2:4371424/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  1:399389/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  2:1491661/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  1:2537166/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            >  1:4116419/1‑93 (MQ=255)

                                               agagactaagactGTAATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACGGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          <  2:10744/91‑1 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          >  2:7294145/1‑93 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          <  1:5020080/93‑1 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  1:6034750/93‑1 (MQ=255)

                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  2:4015939/93‑1 (MQ=255)

                                                  gactaagactGTCATGAAAAAGACCAGAATTGTATGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       <  2:8962672/93‑1 (MQ=255)

                                                  gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       >  1:1564852/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     >  2:1244499/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     >  1:9469307/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     <  1:6857576/93‑1 (MQ=255)

                                                     taagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                    <  2:6228431/93‑1 (MQ=255)

                                                      aagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                                   <  2:5867202/93‑1 (MQ=255)

                                                        gactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGaa                                 <  1:1058951/93‑1 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                <  1:1485534/93‑1 (MQ=255)

                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               >  1:6247376/1‑93 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  1:7961912/1‑93 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             >  1:985844/1‑93 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:4480098/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:7056012/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:8414020/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            >  1:2724259/1‑93 (MQ=255)

                                                              cATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                           >  2:6011869/1‑93 (MQ=255)

                                                               aTGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                          <  2:1256921/93‑1 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  2:7626411/1‑93 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:9711674/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:7927949/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:4305961/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      >  2:8616226/1‑93 (MQ=255)

                                                                      aGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                   <  2:7376369/93‑1 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:8119442/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:2462539/1‑93 (MQ=255)

                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaat                 >  1:7656043/1‑92 (MQ=255)

                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAc                 >  2:1164970/1‑93 (MQ=255)

                                                                         ccAAAATTGTTTGCACCGTCGGACCGAAAACCGCATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGGCTGAACt                >  1:7223385/1‑93 (MQ=255)

                                                                         ccAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACt                >  1:3411250/1‑93 (MQ=255)

                                                                          cAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtc               >  1:5666850/1‑92 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  2:7188312/1‑93 (MQ=255)

                                                                              aatGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGCACTtctct           >  1:9183604/3‑93 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGCCGCTGGCATGAAACTTATGCGTCTGAACTtctctc          <  1:1815453/93‑1 (MQ=255)

                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         <  1:572554/93‑1 (MQ=255)

                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         >  2:5739919/1‑93 (MQ=255)

                                                                                 gTTTGCACCGTCGTACCGAAAACCGAATCTGAATAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt        >  2:7699927/1‑93 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  2:8086733/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  1:8820856/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  1:216216/93‑1 (MQ=255)

                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     >  2:3743660/1‑93 (MQ=255)

                                                                                       aCCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAc  <  2:3970882/93‑1 (MQ=255)

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753656‑1753842

CTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCG                                                                                               <  SRR4302540.2700631/94‑1 (MQ=60)

 TTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGT                                                                                              >  SRR4302540.4115685/1‑94 (MQ=60)

   TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCG                                                                                            <  SRR4302540.7975457/94‑1 (MQ=60)

    CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           <  SRR4302540.1144706/94‑1 (MQ=60)

    CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           <  SRR4302540.8662823/94‑1 (MQ=60)

     CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGA                                                                                          >  SRR4302540.1695184/1‑94 (MQ=60)

     CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGA                                                                                          >  SRR4302540.1586130/1‑94 (MQ=60)

     CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGA                                                                                          <  SRR4302540.6788489/94‑1 (MQ=60)

       GCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACAAAAATTGTTTGCACCGTCGGACC                                                                                        <  SRR4302540.1854706/94‑1 (MQ=60)

        CAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCG                                                                                       >  SRR4302540.2511586/1‑94 (MQ=60)

         AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302540.2428543/1‑94 (MQ=60)

         AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302540.9336077/1‑94 (MQ=60)

         AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302540.6250734/1‑94 (MQ=60)

           TGCGCCCAGAAAGCAAGTTTCTCCCATCCTTATCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGCCGGACCGAAA                                                                                    >  SRR4302540.1582109/1‑94 (MQ=60)

             CGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAC                                                                                  >  SRR4302540.2499305/1‑94 (MQ=60)

              GCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACC                                                                                 <  SRR4302540.2405261/94‑1 (MQ=60)

              GCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACC                                                                                 >  SRR4302540.1453117/1‑94 (MQ=60)

              GCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACC                                                                                 >  SRR4302540.1453118/1‑94 (MQ=60)

               CCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCG                                                                                >  SRR4302540.7818491/1‑94 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              <  SRR4302540.8949920/94‑1 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              >  SRR4302540.5096268/1‑94 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              <  SRR4302540.5434170/94‑1 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              <  SRR4302540.8140787/94‑1 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              >  SRR4302540.53143/1‑94 (MQ=60)

                 CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              <  SRR4302540.1443662/94‑1 (MQ=60)

                  AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGCCTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302540.3860546/94‑1 (MQ=60)

                  AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAATACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCAAAT                                                                             >  SRR4302540.3202936/1‑94 (MQ=60)

                  AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             >  SRR4302540.5562182/1‑94 (MQ=60)

                  AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             >  SRR4302540.3202938/1‑94 (MQ=60)

                  AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302540.3265925/94‑1 (MQ=60)

                    AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           <  SRR4302540.110162/94‑1 (MQ=60)

                    AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           <  SRR4302540.7566164/94‑1 (MQ=60)

                    AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           >  SRR4302540.2619561/1‑94 (MQ=60)

                      AGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGA                                                                         <  SRR4302540.7189899/94‑1 (MQ=60)

                      AGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGA                                                                         <  SRR4302540.1876551/94‑1 (MQ=60)

                      AGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGA                                                                         >  SRR4302540.5091927/1‑94 (MQ=60)

                       GCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAA                                                                        >  SRR4302540.1697802/1‑94 (MQ=60)

                       GCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAA                                                                        <  SRR4302540.198338/94‑1 (MQ=60)

                        CAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAG                                                                       >  SRR4302540.4255370/1‑94 (MQ=60)

                         AAGTTTCTCCCATCCTTCTCAACTTAAAGACT‑AGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     <  SRR4302540.758427/94‑1 (MQ=60)

                          AGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     <  SRR4302540.292361/94‑1 (MQ=60)

                          AGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAG                                                                     >  SRR4302540.5284265/1‑94 (MQ=60)

                           GTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGA                                                                    >  SRR4302540.8180599/1‑94 (MQ=60)

                           GTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGA                                                                    >  SRR4302540.8899096/1‑94 (MQ=60)

                            TTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAT                                                                   <  SRR4302540.9530039/94‑1 (MQ=60)

                            TTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAT                                                                   <  SRR4302540.119890/94‑1 (MQ=60)

                            TTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAT                                                                   <  SRR4302540.2973025/94‑1 (MQ=60)

                             TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  >  SRR4302540.5065701/1‑94 (MQ=60)

                             TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  >  SRR4302540.5643913/1‑94 (MQ=60)

                              TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 <  SRR4302540.3173613/94‑1 (MQ=60)

                              TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 >  SRR4302540.3506906/1‑94 (MQ=60)

                              TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 >  SRR4302540.638510/1‑94 (MQ=60)

                              TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 <  SRR4302540.4443511/94‑1 (MQ=60)

                              TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 <  SRR4302540.4395865/94‑1 (MQ=60)

                               CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTT                                                                >  SRR4302540.8559821/1‑94 (MQ=60)

                                TCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTA                                                               >  SRR4302540.8774952/1‑94 (MQ=60)

                                TCCCATCCTTCTCAACCTAAAGACTAAGACTGTCATGAAAAAGCCCAAAATTGTTTGCACCGCCGGACCGAAAACCTAATCTGAAGAGATGTCA                                                               >  SRR4302540.1425974/1‑94 (MQ=60)

                                 CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              >  SRR4302540.5208187/1‑94 (MQ=60)

                                 CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              <  SRR4302540.7079915/94‑1 (MQ=60)

                                  CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACTGTAGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302540.1924405/1‑94 (MQ=60)

                                  CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302540.643744/1‑94 (MQ=60)

                                  CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302540.1373011/1‑94 (MQ=60)

                                   CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            >  SRR4302540.5981532/1‑94 (MQ=60)

                                   CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            <  SRR4302540.3140944/94‑1 (MQ=60)

                                   CATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCT                                                            >  SRR4302540.933732/1‑94 (MQ=60)

                                    ATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGACAACCGAATCTCACGAGATGTTAGCTA                                                           >  SRR4302540.5021163/1‑94 (MQ=60)

                                      CCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAA                                                         <  SRR4302540.229880/94‑1 (MQ=60)

                                       CTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAA                                                        >  SRR4302540.1203329/1‑94 (MQ=60)

                                       CTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAA                                                        <  SRR4302540.724472/94‑1 (MQ=60)

                                        TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       >  SRR4302540.5616706/1‑94 (MQ=60)

                                        TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       <  SRR4302540.7098658/94‑1 (MQ=60)

                                        TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       <  SRR4302540.9167097/94‑1 (MQ=60)

                                        TTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAT                                                       <  SRR4302540.9167343/94‑1 (MQ=60)

                                         TCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATG                                                      <  SRR4302540.1580940/94‑1 (MQ=60)

                                         TCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATG                                                      >  SRR4302540.4915103/1‑94 (MQ=60)

                                         TCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATG                                                      >  SRR4302540.8145663/1‑94 (MQ=60)

                                         TCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATG                                                      >  SRR4302540.6475692/1‑94 (MQ=60)

                                          CTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGC                                                     >  SRR4302540.4339114/1‑94 (MQ=60)

                                          CTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGC                                                     >  SRR4302540.8638624/1‑94 (MQ=60)

                                          CTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGC                                                     >  SRR4302540.1124719/1‑94 (MQ=60)

                                           TCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCT                                                    <  SRR4302540.2849839/94‑1 (MQ=60)

                                            CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302540.8063262/94‑1 (MQ=60)

                                            CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302540.8063261/94‑1 (MQ=60)

                                            CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302540.2966739/94‑1 (MQ=60)

                                            CAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTG                                                   <  SRR4302540.8299004/94‑1 (MQ=60)

                                             AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  >  SRR4302540.4886814/1‑94 (MQ=60)

                                             AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  >  SRR4302540.4357559/1‑94 (MQ=60)

                                               CTTAAAGACTAAGGCTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.5414185/94‑1 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.2796939/94‑1 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.2564527/94‑1 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                >  SRR4302540.4160861/1‑94 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.403542/94‑1 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.1145684/94‑1 (MQ=60)

                                               CTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTCTTTGCACCGTCGGACCGAAACCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAC                                                <  SRR4302540.2283603/94‑1 (MQ=60)

                                                TTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACG                                               <  SRR4302540.5408029/94‑1 (MQ=60)

                                                  AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTTGCTAAAATGCTGGACGCT                                             >  SRR4302540.56296/1‑94 (MQ=60)

                                                  AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCT                                             <  SRR4302540.4492946/94‑1 (MQ=60)

                                                  AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCT                                             <  SRR4302540.5074427/94‑1 (MQ=60)

                                                   AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTG                                            <  SRR4302540.6101733/94‑1 (MQ=60)

                                                    AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           >  SRR4302540.8021458/1‑94 (MQ=60)

                                                    AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           <  SRR4302540.1725296/94‑1 (MQ=60)

                                                    AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           >  SRR4302540.1582027/1‑94 (MQ=60)

                                                      ACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCA                                         >  SRR4302540.9581628/1‑94 (MQ=60)

                                                       CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        >  SRR4302540.7978234/1‑94 (MQ=60)

                                                       CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        <  SRR4302540.6933565/94‑1 (MQ=60)

                                                       CTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAT                                        >  SRR4302540.8113968/1‑94 (MQ=60)

                                                        TAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATG                                       >  SRR4302540.5019073/1‑94 (MQ=60)

                                                        TAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATG                                       >  SRR4302540.5018771/1‑94 (MQ=60)

                                                          AGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAA                                     <  SRR4302540.907520/94‑1 (MQ=60)

                                                           GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    <  SRR4302540.1069731/94‑1 (MQ=60)

                                                            ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   <  SRR4302540.1171611/94‑1 (MQ=60)

                                                            ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   <  SRR4302540.1501843/94‑1 (MQ=60)

                                                            ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   >  SRR4302540.6316897/1‑94 (MQ=60)

                                                             CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  <  SRR4302540.153380/94‑1 (MQ=60)

                                                             CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  >  SRR4302540.8054704/1‑94 (MQ=60)

                                                             CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  <  SRR4302540.3958577/94‑1 (MQ=60)

                                                             CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  >  SRR4302540.4102865/1‑94 (MQ=60)

                                                              TGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTT                                 >  SRR4302540.995920/1‑94 (MQ=60)

                                                              TGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTT                                 >  SRR4302540.5908211/1‑94 (MQ=60)

                                                               GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                >  SRR4302540.2753633/1‑94 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.5737926/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.4528683/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.7134479/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.5044313/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.8511683/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.8511682/94‑1 (MQ=60)

                                                                TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302540.5044312/94‑1 (MQ=60)

                                                                 CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              <  SRR4302540.3921586/94‑1 (MQ=60)

                                                                 CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              <  SRR4302540.4598026/94‑1 (MQ=60)

                                                                  ATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGC                             <  SRR4302540.989389/94‑1 (MQ=60)

                                                                    GAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGT                           >  SRR4302540.5077056/1‑94 (MQ=60)

                                                                     AAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTC                          >  SRR4302540.3098862/1‑94 (MQ=60)

                                                                     AAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTC                          >  SRR4302540.7492812/1‑94 (MQ=60)

                                                                      AAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT                         <  SRR4302540.3377704/94‑1 (MQ=60)

                                                                      AAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT                         >  SRR4302540.6695158/1‑94 (MQ=60)

                                                                         AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302540.7818159/1‑94 (MQ=60)

                                                                         AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302540.2489041/1‑94 (MQ=60)

                                                                         AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      >  SRR4302540.8213668/1‑94 (MQ=60)

                                                                         AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      <  SRR4302540.4059212/94‑1 (MQ=60)

                                                                          GACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAT                     >  SRR4302540.7742244/1‑94 (MQ=60)

                                                                          GACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAC                     <  SRR4302540.1721247/94‑1 (MQ=60)

                                                                          GACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAC                     <  SRR4302540.6381818/94‑1 (MQ=60)

                                                                           ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGCATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGGCTGAACT                    >  SRR4302540.7303909/1‑94 (MQ=60)

                                                                           ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACT                    >  SRR4302540.3448403/1‑94 (MQ=60)

                                                                           ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACT                    >  SRR4302540.7303910/1‑94 (MQ=60)

                                                                            CCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTC                   >  SRR4302540.5729256/1‑94 (MQ=60)

                                                                             CAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCGGAACTTC                  >  SRR4302540.3616744/1‑94 (MQ=60)

                                                                              AAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCT                 <  SRR4302540.9682490/94‑1 (MQ=60)

                                                                              AAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCT                 <  SRR4302540.2321752/94‑1 (MQ=60)

                                                                              AAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCT                 >  SRR4302540.8430008/1‑94 (MQ=60)

                                                                               AAATTGTTTGCACCGTCTGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTC                <  SRR4302540.10052848/94‑1 (MQ=60)

                                                                                AATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGCTGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTatat               >  SRR4302540.1851941/1‑90 (MQ=60)

                                                                                AAATGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGCACTTCTCT               >  SRR4302540.9293744/1‑94 (MQ=60)

                                                                                 ATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTCAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTC              >  SRR4302540.2645401/1‑94 (MQ=60)

                                                                                 ATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTC              >  SRR4302540.7343875/1‑94 (MQ=60)

                                                                                  TTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGCCGCTGGCATGAAACTTATGCGTCTGAACTTCTCTCA             <  SRR4302540.1835275/94‑1 (MQ=60)

                                                                                   TGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAT            <  SRR4302540.578769/94‑1 (MQ=60)

                                                                                   TGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAT            <  SRR4302540.355592/94‑1 (MQ=60)

                                                                                     TTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGG          <  SRR4302540.7736760/94‑1 (MQ=60)

                                                                                     TTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGG          <  SRR4302540.8922849/94‑1 (MQ=60)

                                                                                     TTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGG          <  SRR4302540.218326/94‑1 (MQ=60)

                                                                                     TTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGG          >  SRR4302540.5579405/1‑94 (MQ=60)

                                                                                      TGGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAACGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGT         <  SRR4302540.6297172/94‑1 (MQ=60)

                                                                                        GCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGA       <  SRR4302540.6080405/94‑1 (MQ=60)

                                                                                        GCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGA       >  SRR4302540.5748478/1‑94 (MQ=60)

                                                                                         CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      <  SRR4302540.9280233/94‑1 (MQ=60)

                                                                                         CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      >  SRR4302540.5402738/1‑94 (MQ=60)

                                                                                          ACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACT     >  SRR4302540.2228260/1‑94 (MQ=60)

                                                                                          ACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACT     <  SRR4302540.2252424/94‑1 (MQ=60)

                                                                                           CCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTA    <  SRR4302540.868951/94‑1 (MQ=60)

                                                                                            CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   <  SRR4302540.7067453/94‑1 (MQ=60)

                                                                                             GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  SRR4302540.6259523/1‑94 (MQ=60)

                                                                                             GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  <  SRR4302540.5819570/94‑1 (MQ=60)

CTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753656‑1753842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`