Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,236	C→T	E301K (GAA→AAA)	ppsA ←	phosphoenolpyruvate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,236	0	C	T	100.0%	749.1 / NA	200	E301K (GAA→AAA)	ppsA	phosphoenolpyruvate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (103/97); total (103/97)

Only 100 of 207 total aligned reads displayed.

ACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG  >  NC_000913/1784146‑1784325

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         >  2:2607367/1‑93 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         <  2:5724694/93‑1 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         >  1:292155/1‑93 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACAGCCTGTTTTGCCAGTTTCt                                                                                         >  2:496300/1‑93 (MQ=255)

 cAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTg                                                                                        <  1:1019198/93‑1 (MQ=255)

 cAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTAAGGCCTGTTTTGCCAGTTTCTg                                                                                        >  2:2710424/1‑93 (MQ=255)

   gTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCa                                                                                      <  2:4343580/93‑1 (MQ=255)

   gTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCa                                                                                      <  1:1709676/93‑1 (MQ=255)

    tttACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAc                                                                                     >  1:2959870/1‑93 (MQ=255)

    attACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAc                                                                                     <  1:6222816/92‑1 (MQ=255)

     ttACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAct                                                                                    <  2:50718/93‑1 (MQ=255)

         cGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCActtctt                                                                                <  2:1729549/93‑1 (MQ=255)

          ggTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACttcttc                                                                               <  1:2293916/93‑1 (MQ=255)

           gtgtGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCg                                                                              >  1:3047304/1‑93 (MQ=255)

            tgtgGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGTCCTGTTTTGCCAGTTTCTGCACTTCTTCGt                                                                             >  1:929955/1‑93 (MQ=255)

             gtgGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGtt                                                                            <  2:3654153/93‑1 (MQ=255)

               ggCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTgg                                                                          >  1:2252239/1‑93 (MQ=255)

                 ccATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTc                                                                        >  1:543139/1‑93 (MQ=255)

                 ccATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTc                                                                        <  1:1825935/93‑1 (MQ=255)

                  cATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCa                                                                       >  1:3347679/1‑93 (MQ=255)

                   aTCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAg                                                                      <  1:4741328/93‑1 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     >  1:1902628/1‑93 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     <  2:459939/93‑1 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     >  2:645908/1‑93 (MQ=255)

                         cGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaa                                                                <  2:3547511/93‑1 (MQ=255)

                          gCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaag                                                               >  2:3148611/1‑93 (MQ=255)

                           cccACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaaga                                                              <  2:2492428/93‑1 (MQ=255)

                            ccACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGAt                                                             >  1:5382703/1‑93 (MQ=255)

                             cACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATg                                                            >  2:3603366/1‑93 (MQ=255)

                             cACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATg                                                            >  2:3772538/1‑93 (MQ=255)

                              aCTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGt                                                           >  1:4305763/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGCTGTc                                                          >  1:3854209/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          >  1:5595795/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          <  2:5731682/93‑1 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          <  1:4961216/93‑1 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          >  1:234559/1‑93 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         <  1:6120809/93‑1 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         <  2:4628132/93‑1 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         >  1:273081/1‑93 (MQ=255)

                                 cAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCAc                                                        <  2:2189994/93‑1 (MQ=255)

                                 cAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCAc                                                        <  2:3996387/93‑1 (MQ=255)

                                  aaTATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACg                                                       >  1:4031346/1‑93 (MQ=255)

                                   atatCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGc                                                      <  2:6284685/93‑1 (MQ=255)

                                    tatCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCt                                                     >  1:1486812/1‑93 (MQ=255)

                                     atCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTg                                                    >  2:1360366/1‑93 (MQ=255)

                                     atCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTg                                                    >  1:1180877/1‑93 (MQ=255)

                                       ccATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGtt                                                  >  1:1283792/1‑93 (MQ=255)

                                          tCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCt                                               <  2:4756942/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  1:5004205/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  1:3804630/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  2:1028867/93‑1 (MQ=255)

                                               cGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGt                                          <  1:920946/93‑1 (MQ=255)

                                                 aCCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGCAGATGTCACGCTGTTCCTGCGGTAc                                        >  1:1557300/1‑93 (MQ=255)

                                                 aCCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTAc                                        >  2:4514779/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  2:3015993/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  2:1495284/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  1:2166570/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  1:4123676/1‑93 (MQ=255)

                                                      aGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCtt                                   <  2:5065780/93‑1 (MQ=255)

                                                      aGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCtt                                   >  2:5056836/1‑93 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  <  1:3178676/93‑1 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  >  1:3468063/1‑93 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  >  2:3863729/1‑93 (MQ=255)

                                                        tgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCg                                 <  1:4359263/93‑1 (MQ=255)

                                                         gtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGa                                >  2:842894/1‑93 (MQ=255)

                                                         gtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGa                                >  2:551324/1‑93 (MQ=255)

                                                          tttCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAt                               >  2:3295305/1‑93 (MQ=255)

                                                            tctcAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAttt                             <  2:4113776/93‑1 (MQ=255)

                                                            tctcAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAttt                             >  1:469530/1‑93 (MQ=255)

                                                               cAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTaa                          >  1:4472775/1‑93 (MQ=255)

                                                                aaTTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAc                         <  2:3972015/93‑1 (MQ=255)

                                                                 aTTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAcc                        <  1:4631442/93‑1 (MQ=255)

                                                                  tttGTGCGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       <  1:3093777/93‑1 (MQ=255)

                                                                  tttGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       <  2:5343913/93‑1 (MQ=255)

                                                                  tttGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       >  2:3257409/1‑93 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    <  2:1675868/93‑1 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    >  1:5950850/1‑93 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    <  2:2110326/93‑1 (MQ=255)

                                                                        cGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGc                 <  1:716321/93‑1 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  2:4220076/1‑93 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  1:37435/1‑93 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  2:231287/1‑93 (MQ=255)

                                                                          gCCTGTTTTGCCAGTTTCTTCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCg               <  2:2572141/93‑1 (MQ=255)

                                                                          gCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCg               >  2:5126001/1‑93 (MQ=255)

                                                                           ccTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGt              <  2:3387022/93‑1 (MQ=255)

                                                                           ccTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGt              >  2:5878058/1‑93 (MQ=255)

                                                                            cTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTg             >  2:2771661/1‑93 (MQ=255)

                                                                             tGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGc            >  2:3050632/1‑93 (MQ=255)

                                                                             tGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGc            >  2:1922343/1‑93 (MQ=255)

                                                                               ttttGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTc          >  1:5847969/1‑93 (MQ=255)

                                                                               ttttGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTc          <  1:142898/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:1467907/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:5649607/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  1:5160575/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:2065049/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  2:342420/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  1:4866145/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    >  2:3685243/1‑93 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  2:6092326/93‑1 (MQ=255)

                                                                                      aGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTc   <  1:653718/93‑1 (MQ=255)

ACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG  >  NC_000913/1784146‑1784325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGC  >  NC_000913/1784143‑1784329

TGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTT                                                                                               >  SRR4302533.4757224/1‑94 (MQ=60)

AGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTT                                                                                               <  SRR4302533.857247/94‑1 (MQ=60)

  AACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCT                                                                                             >  SRR4302533.293939/1‑94 (MQ=60)

   ACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTG                                                                                            >  SRR4302533.5034174/1‑94 (MQ=60)

    CAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGC                                                                                           <  SRR4302533.3855604/94‑1 (MQ=60)

    CAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGC                                                                                           <  SRR4302533.1025188/94‑1 (MQ=60)

     AGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCA                                                                                          >  SRR4302533.5417511/1‑94 (MQ=60)

      GTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAC                                                                                         <  SRR4302533.1720037/94‑1 (MQ=60)

      GTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAC                                                                                         >  SRR4302533.2977962/1‑94 (MQ=60)

       ATTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACT                                                                                        <  SRR4302533.6260110/94‑1 (MQ=60)

         TACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTC                                                                                      >  SRR4302533.4579392/1‑94 (MQ=60)

          TCCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCT                                                                                     <  SRR4302533.2819254/94‑1 (MQ=60)

          ACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCT                                                                                     <  SRR4302533.2202173/94‑1 (MQ=60)

           CCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTT                                                                                    >  SRR4302533.3176815/1‑94 (MQ=60)

            CGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTC                                                                                   >  SRR4302533.810725/1‑94 (MQ=60)

            CGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTC                                                                                   <  SRR4302533.14962/94‑1 (MQ=60)

            CGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTC                                                                                   >  SRR4302533.2665431/1‑94 (MQ=60)

             GGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCG                                                                                  >  SRR4302533.3065961/1‑94 (MQ=60)

             GGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCG                                                                                  <  SRR4302533.2308118/94‑1 (MQ=60)

              GTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGTCCTGTTTTGCCAGTTTCTGCACTTCTTCGT                                                                                 >  SRR4302533.935413/1‑94 (MQ=60)

              GTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGT                                                                                 >  SRR4302533.945729/1‑94 (MQ=60)

               TGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTT                                                                                <  SRR4302533.4838406/94‑1 (MQ=60)

               TGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTT                                                                                >  SRR4302533.1962667/1‑94 (MQ=60)

                 TGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGG                                                                              >  SRR4302533.2266165/1‑94 (MQ=60)

                  GGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGT                                                                             >  SRR4302533.4195888/1‑94 (MQ=60)

                   GCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTC                                                                            >  SRR4302533.546362/1‑94 (MQ=60)

                    CCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCA                                                                           >  SRR4302533.1865534/1‑94 (MQ=60)

                    CCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCA                                                                           <  SRR4302533.1837087/94‑1 (MQ=60)

                    CCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCA                                                                           >  SRR4302533.3368363/1‑94 (MQ=60)

                     CATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAG                                                                          >  SRR4302533.646551/1‑94 (MQ=60)

                      ATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGC                                                                         >  SRR4302533.1914240/1‑94 (MQ=60)

                      ATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGC                                                                         <  SRR4302533.4770074/94‑1 (MQ=60)

                      ATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGC                                                                         >  SRR4302533.4496233/1‑94 (MQ=60)

                        CTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGA                                                                       >  SRR4302533.934446/1‑94 (MQ=60)

                            CGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAG                                                                   >  SRR4302533.4370163/1‑94 (MQ=60)

                              CCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGAT                                                                 <  SRR4302533.3814772/94‑1 (MQ=60)

                              CCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGAT                                                                 >  SRR4302533.5415041/1‑94 (MQ=60)

                                CACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGT                                                               >  SRR4302533.4331670/1‑94 (MQ=60)

                                 ACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGCTGTC                                                              >  SRR4302533.3877749/1‑94 (MQ=60)

                                 ACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTC                                                              >  SRR4302533.236007/1‑94 (MQ=60)

                                 ACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTC                                                              >  SRR4302533.5629607/1‑94 (MQ=60)

                                  CTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCA                                                             >  SRR4302533.274743/1‑94 (MQ=60)

                                  CTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCA                                                             <  SRR4302533.4991299/94‑1 (MQ=60)

                                   TCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCAC                                                            <  SRR4302533.6157718/94‑1 (MQ=60)

                                    CAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACG                                                           >  SRR4302533.4055886/1‑94 (MQ=60)

                                    CAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACG                                                           >  SRR4302533.1071627/1‑94 (MQ=60)

                                      ATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCT                                                         >  SRR4302533.1495781/1‑94 (MQ=60)

                                       TATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTG                                                        >  SRR4302533.1187970/1‑94 (MQ=60)

                                       TATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTG                                                        >  SRR4302533.6257212/1‑94 (MQ=60)

                                         TCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTT                                                      >  SRR4302533.1291516/1‑94 (MQ=60)

                                             TCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTG                                                  <  SRR4302533.2349640/94‑1 (MQ=60)

                                             TCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTG                                                  <  SRR4302533.3088866/94‑1 (MQ=60)

                                              CGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGC                                                 >  SRR4302533.678244/1‑94 (MQ=60)

                                                GGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGG                                               <  SRR4302533.5839763/94‑1 (MQ=60)

                                                 GCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGT                                              <  SRR4302533.5034532/94‑1 (MQ=60)

                                                 GCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGT                                              >  SRR4302533.2970695/1‑94 (MQ=60)

                                                 GCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGT                                              <  SRR4302533.3827910/94‑1 (MQ=60)

                                                  CGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTA                                             >  SRR4302533.3255957/1‑94 (MQ=60)

                                                  CGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTA                                             <  SRR4302533.926353/94‑1 (MQ=60)

                                                   GACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGCAGATGTCACGCTGTTCCTGCGGTAC                                            >  SRR4302533.1566673/1‑94 (MQ=60)

                                                   GACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTAC                                            <  SRR4302533.5961237/94‑1 (MQ=60)

                                                   GACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTAC                                            <  SRR4302533.4715940/94‑1 (MQ=60)

                                                   GACCGTAGTGTTTCTCAATTTGTACGGCCTGTTGTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTAC                                            <  SRR4302533.1373926/94‑1 (MQ=60)

                                                    ACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACG                                           >  SRR4302533.2958654/1‑94 (MQ=60)

                                                       GTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCT                                        >  SRR4302533.2179896/1‑94 (MQ=60)

                                                       GTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCT                                        >  SRR4302533.657125/1‑94 (MQ=60)

                                                       GTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCT                                        >  SRR4302533.4148712/1‑94 (MQ=60)

                                                         AGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTC                                      >  SRR4302533.3489471/1‑94 (MQ=60)

                                                          GTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCG                                     <  SRR4302533.3198229/94‑1 (MQ=60)

                                                           TGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGA                                    <  SRR4302533.4385576/94‑1 (MQ=60)

                                                             TTTCTCAATTTTTACGGCCTTTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAACGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATT                                  <  SRR4302533.4634413/94‑1 (MQ=60)

                                                             TTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATT                                  <  SRR4302533.5600045/94‑1 (MQ=60)

                                                              TTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTT                                 >  SRR4302533.472335/1‑94 (MQ=60)

                                                                 TCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAA                              >  SRR4302533.4499828/1‑94 (MQ=60)

                                                                  CAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAT                             >  SRR4302533.3720989/1‑94 (MQ=60)

                                                                  CAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAC                             >  SRR4302533.5325188/1‑94 (MQ=60)

                                                                    ATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCT                           <  SRR4302533.4659509/94‑1 (MQ=60)

                                                                     TTTGTGCGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTG                          <  SRR4302533.3112799/94‑1 (MQ=60)

                                                                     TTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTG                          <  SRR4302533.4751203/94‑1 (MQ=60)

                                                                       TGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCT                        >  SRR4302533.5986845/1‑94 (MQ=60)

                                                                         TACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTG                      <  SRR4302533.1720872/94‑1 (MQ=60)

                                                                          ACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGTTTGC                     >  SRR4302533.1603979/1‑94 (MQ=60)

                                                                          ACGGCCTGTTATGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTCCCTACGGTACGTCTACGATTTTAACCTGCTTGC                     <  SRR4302533.5999862/94‑1 (MQ=60)

                                                                           CGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCC                    >  SRR4302533.37683/1‑94 (MQ=60)

                                                                           CGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCC                    <  SRR4302533.3061499/94‑1 (MQ=60)

                                                                           CGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCC                    <  SRR4302533.720544/94‑1 (MQ=60)

                                                                             GCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGT                  >  SRR4302533.5048846/1‑94 (MQ=60)

                                                                               CTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGC                >  SRR4302533.4086724/1‑94 (MQ=60)

                                                                               CTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGC                >  SRR4302533.3413412/1‑94 (MQ=60)

                                                                                 GTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTC              <  SRR4302533.3989628/94‑1 (MQ=60)

                                                                                 GTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTC              >  SRR4302533.5883320/1‑94 (MQ=60)

                                                                                  TTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCC             <  SRR4302533.143810/94‑1 (MQ=60)

                                                                                   TTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCT            <  SRR4302533.1220979/94‑1 (MQ=60)

                                                                                     TGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGG          <  SRR4302533.5191720/94‑1 (MQ=60)

                                                                                      GCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGG         <  SRR4302533.2307815/94‑1 (MQ=60)

                                                                                        CAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTC       <  SRR4302533.4895634/94‑1 (MQ=60)

                                                                                         AGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG      >  SRR4302533.4952619/1‑94 (MQ=60)

                                                                                         AGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG      <  SRR4302533.657576/94‑1 (MQ=60)

                                                                                         AGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG      >  SRR4302533.1559427/1‑94 (MQ=60)

                                                                                           TTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGC    >  SRR4302533.2927176/1‑94 (MQ=60)

                                                                                            TTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCG   <  SRR4302533.6096140/94‑1 (MQ=60)

                                                                                             TCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGC  >  SRR4302533.224501/1‑94 (MQ=60)

TGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGC  >  NC_000913/1784143‑1784329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`