Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,799,569 G→A N342N (AAC→AAT)  thrS ← threonine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,5690GA100.0% 661.1 / NA 178N342N (AAC→AAT) thrSthreonine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (79/99);  total (79/99)

Only 100 of 181 total aligned reads displayed.

GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658
                                                                                          |                                                                                         
gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         <  1:5048203/93‑1 (MQ=255)
gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         <  1:1702351/93‑1 (MQ=255)
  cATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGa                                                                                       <  1:2895651/93‑1 (MQ=255)
   aTGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaa                                                                                      >  1:12899/1‑93 (MQ=255)
    tGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaaa                                                                                     <  2:1194532/93‑1 (MQ=255)
    tGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaaa                                                                                     <  1:2458639/93‑1 (MQ=255)
     gCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaaaa                                                                                    >  1:1454970/1‑93 (MQ=255)
        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 >  1:3896234/1‑93 (MQ=255)
           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  1:2680560/93‑1 (MQ=255)
              cTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGt                                                                           <  1:3744248/93‑1 (MQ=255)
               tGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTg                                                                          >  2:5109398/1‑93 (MQ=255)
                gACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGa                                                                         >  2:2794068/1‑93 (MQ=255)
                gACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGa                                                                         <  1:2295357/93‑1 (MQ=255)
                 aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAc                                                                        >  1:4485915/1‑93 (MQ=255)
                   ggCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAccc                                                                      <  1:5775690/93‑1 (MQ=255)
                     cTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCgg                                                                    >  1:1158716/1‑93 (MQ=255)
                      tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCggg                                                                   >  1:3236919/1‑93 (MQ=255)
                      tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCggg                                                                   <  1:5963178/93‑1 (MQ=255)
                       cGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGc                                                                  >  2:3462222/1‑93 (MQ=255)
                        gTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCa                                                                 >  1:5437151/1‑93 (MQ=255)
                         ttACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAg                                                                >  2:275149/1‑93 (MQ=255)
                         ttACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAg                                                                >  1:1321838/1‑93 (MQ=255)
                         ttACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAg                                                                >  2:1965091/1‑93 (MQ=255)
                          tACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGt                                                               <  1:5857296/93‑1 (MQ=255)
                          tACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGt                                                               >  2:1265092/1‑93 (MQ=255)
                           aCGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGtt                                                              >  1:5529833/1‑93 (MQ=255)
                           aCGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGtt                                                              <  1:3531261/93‑1 (MQ=255)
                            cGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTc                                                             >  2:2694295/1‑93 (MQ=255)
                             ggTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCa                                                            >  1:3500837/1‑93 (MQ=255)
                              gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAt                                                           <  1:1094576/93‑1 (MQ=255)
                               tGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATc                                                          <  1:2614714/93‑1 (MQ=255)
                                ggCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCg                                                         <  2:5541142/93‑1 (MQ=255)
                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        >  1:5193325/1‑93 (MQ=255)
                                   aGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCt                                                      >  1:308644/1‑93 (MQ=255)
                                    gCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCtt                                                     >  1:2528739/1‑93 (MQ=255)
                                      tACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTaa                                                   >  2:2208162/1‑93 (MQ=255)
                                      tACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTaa                                                   >  2:5453962/1‑93 (MQ=255)
                                      tACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTaa                                                   <  1:4593090/93‑1 (MQ=255)
                                       aCCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAt                                                  <  2:1389551/93‑1 (MQ=255)
                                       aCCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAt                                                  >  1:2222565/1‑93 (MQ=255)
                                         cAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGc                                                <  1:2562448/93‑1 (MQ=255)
                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               <  1:1773469/93‑1 (MQ=255)
                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               >  1:2076707/1‑93 (MQ=255)
                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               <  1:320736/93‑1 (MQ=255)
                                           aaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAg                                              <  2:6297950/93‑1 (MQ=255)
                                            aCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGt                                             <  2:3757139/93‑1 (MQ=255)
                                            aCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGt                                             >  2:4687669/1‑93 (MQ=255)
                                            aCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGt                                             <  1:648021/93‑1 (MQ=255)
                                              tCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAt                                           <  1:5744903/93‑1 (MQ=255)
                                                ggCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTc                                         >  2:1850336/1‑93 (MQ=255)
                                                ggCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTc                                         <  2:6139937/93‑1 (MQ=255)
                                                ggCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTc                                         <  2:520589/93‑1 (MQ=255)
                                                 gCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCa                                        <  1:3080382/93‑1 (MQ=255)
                                                  ccATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAc                                       >  2:1517351/1‑93 (MQ=255)
                                                   cATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACg                                      <  1:3880650/93‑1 (MQ=255)
                                                    aTACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACgg                                     >  2:3692009/1‑93 (MQ=255)
                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  2:5782998/1‑93 (MQ=255)
                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  2:2622863/1‑93 (MQ=255)
                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  2:6053565/1‑93 (MQ=255)
                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  1:4966396/1‑93 (MQ=255)
                                                      aCGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGtt                                   >  2:910778/1‑93 (MQ=255)
                                                       cGCAGCGGCAGATCGCGATAAGACTTCCGCCCCTGATTGAAAATTTGTACGTGATCCGGGCAGGTCATCGGCTTAAAGCAGTATTCATGGTtc                                  >  1:2748666/1‑93 (MQ=255)
                                                        gCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtct                                 <  2:4092210/93‑1 (MQ=255)
                                                         cAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtctc                                <  1:3948055/93‑1 (MQ=255)
                                                         cAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtctc                                <  1:4820123/93‑1 (MQ=255)
                                                          aGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCa                               >  2:2399630/1‑93 (MQ=255)
                                                           gCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCag                              >  2:5039791/1‑93 (MQ=255)
                                                             ggCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCagaa                            <  1:91860/93‑1 (MQ=255)
                                                             ggCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCagaa                            <  2:5946058/93‑1 (MQ=255)
                                                                 gATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtg                        <  1:3278837/93‑1 (MQ=255)
                                                                   tCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgtg                      >  1:3788159/1‑93 (MQ=255)
                                                                    cgcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtgg                     <  2:3438458/93‑1 (MQ=255)
                                                                    cgcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtgg                     <  2:3870356/93‑1 (MQ=255)
                                                                      cgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtggtg                   <  1:2156447/93‑1 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 <  1:3897016/93‑1 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 >  2:4036805/1‑93 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 <  1:4012903/93‑1 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 >  2:4249775/1‑93 (MQ=255)
                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                >  1:5698028/1‑93 (MQ=255)
                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                >  1:4120860/1‑93 (MQ=255)
                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                <  2:4891965/93‑1 (MQ=255)
                                                                          aaGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACa               <  2:5985149/93‑1 (MQ=255)
                                                                          aaGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACa               <  2:1364179/93‑1 (MQ=255)
                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              <  2:1788312/93‑1 (MQ=255)
                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              <  1:4477833/93‑1 (MQ=255)
                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             <  2:1926052/93‑1 (MQ=255)
                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             <  2:2043445/93‑1 (MQ=255)
                                                                               ttCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCa          >  1:5292329/1‑93 (MQ=255)
                                                                               ttCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCa          <  1:1962561/93‑1 (MQ=255)
                                                                                tCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCAt         <  1:5195357/93‑1 (MQ=255)
                                                                                 cAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATc        <  2:6056004/93‑1 (MQ=255)
                                                                                 cAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATc        >  2:3239829/1‑93 (MQ=255)
                                                                                 cAGCCCCTGATTGAAAATTAGTACGTGACCCGGGCAGTTCATCGGATTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATc        <  1:6305219/93‑1 (MQ=255)
                                                                                  aGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCt       <  2:5864857/93‑1 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  2:5720677/93‑1 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  1:1056257/93‑1 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  2:4414320/93‑1 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  2:663292/93‑1 (MQ=255)
                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  <  2:1603548/93‑1 (MQ=255)
                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  <  1:148040/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTG  >  NC_000913/1799476‑1799662
                                                                                             |                                                                                             
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGACAAGACTTCAGCCCCTGA                                                                                               >  SRR4302533.4815810/1‑94 (MQ=60)
 AGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAT                                                                                              <  SRR4302533.2900719/94‑1 (MQ=60)
 AGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGAGATAAGACTTCAGCCCCTGAT                                                                                              >  SRR4302533.1938293/1‑94 (MQ=60)
 AGGCCATGCAGCGAACCTGACGGCTCGGTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAT                                                                                              >  SRR4302533.2558768/1‑94 (MQ=60)
   GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTG                                                                                            <  SRR4302533.5078739/94‑1 (MQ=60)
   GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTG                                                                                            <  SRR4302533.1712666/94‑1 (MQ=60)
     CATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAA                                                                                          <  SRR4302533.2913459/94‑1 (MQ=60)
     CATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAA                                                                                          >  SRR4302533.12995/1‑94 (MQ=60)
     CATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACCTCAGCCCCTGATTGTA                                                                                          >  SRR4302533.134877/1‑94 (MQ=60)
       TGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAA                                                                                        <  SRR4302533.2473811/94‑1 (MQ=60)
       TGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAA                                                                                        >  SRR4302533.1463743/1‑94 (MQ=60)
        GCAGCGACCTTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAT                                                                                       <  SRR4302533.412841/94‑1 (MQ=60)
        GCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAT                                                                                       <  SRR4302533.4947155/94‑1 (MQ=60)
          AGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTT                                                                                     >  SRR4302533.3920007/1‑94 (MQ=60)
             GAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTA                                                                                  >  SRR4302533.5846552/1‑94 (MQ=60)
             GAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTA                                                                                  >  SRR4302533.230314/1‑94 (MQ=60)
              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                 <  SRR4302533.2696985/94‑1 (MQ=60)
               ACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACG                                                                                >  SRR4302533.131579/1‑94 (MQ=60)
                CCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGT                                                                               <  SRR4302533.3036815/94‑1 (MQ=60)
                CCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGT                                                                               <  SRR4302533.5045000/94‑1 (MQ=60)
                 CTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTG                                                                              <  SRR4302533.3767211/94‑1 (MQ=60)
                 CTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTG                                                                              >  SRR4302533.5425239/1‑94 (MQ=60)
                   GACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAC                                                                            >  SRR4302533.4513072/1‑94 (MQ=60)
                   GACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAC                                                                            <  SRR4302533.2309572/94‑1 (MQ=60)
                    ACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACC                                                                           <  SRR4302533.4252452/94‑1 (MQ=60)
                      GGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCG                                                                         >  SRR4302533.5688864/1‑94 (MQ=60)
                      GGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCG                                                                         <  SRR4302533.5810572/94‑1 (MQ=60)
                       GCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGG                                                                        >  SRR4302533.1165670/1‑94 (MQ=60)
                        CTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGG                                                                       <  SRR4302533.5530803/94‑1 (MQ=60)
                        CTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGG                                                                       >  SRR4302533.3256866/1‑94 (MQ=60)
                         TGGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGC                                                                      <  SRR4302533.3767468/94‑1 (MQ=60)
                         TCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGC                                                                      <  SRR4302533.5999254/94‑1 (MQ=60)
                          CGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCA                                                                     >  SRR4302533.5469897/1‑94 (MQ=60)
                           GTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAG                                                                    >  SRR4302533.174710/1‑94 (MQ=60)
                           GTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAG                                                                    >  SRR4302533.1329771/1‑94 (MQ=60)
                             TACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTT                                                                  >  SRR4302533.5563216/1‑94 (MQ=60)
                             TACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTT                                                                  <  SRR4302533.5892704/94‑1 (MQ=60)
                              ACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTC                                                                 <  SRR4302533.3553023/94‑1 (MQ=60)
                               CGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCA                                                                >  SRR4302533.3522424/1‑94 (MQ=60)
                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                               <  SRR4302533.5804944/94‑1 (MQ=60)
                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                               <  SRR4302533.3904309/94‑1 (MQ=60)
                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                               <  SRR4302533.3314114/94‑1 (MQ=60)
                                 GTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATC                                                              <  SRR4302533.1101056/94‑1 (MQ=60)
                                  TGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCG                                                             >  SRR4302533.3916365/1‑94 (MQ=60)
                                  TGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCG                                                             <  SRR4302533.2630764/94‑1 (MQ=60)
                                  TGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCG                                                             <  SRR4302533.3272865/94‑1 (MQ=60)
                                   GGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGG                                                            >  SRR4302533.5224651/1‑94 (MQ=60)
                                     CAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCT                                                          <  SRR4302533.1131874/94‑1 (MQ=60)
                                     CAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCT                                                          >  SRR4302533.310533/1‑94 (MQ=60)
                                      AGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTT                                                         >  SRR4302533.2544308/1‑94 (MQ=60)
                                       GCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTA                                                        <  SRR4302533.4543122/94‑1 (MQ=60)
                                         TACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAT                                                      >  SRR4302533.2236295/1‑94 (MQ=60)
                                         TACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAT                                                      <  SRR4302533.4620912/94‑1 (MQ=60)
                                          ACCAAACCCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATG                                                     <  SRR4302533.1205099/94‑1 (MQ=60)
                                            CAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCA                                                   <  SRR4302533.2578224/94‑1 (MQ=60)
                                            CAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCA                                                   >  SRR4302533.2089488/1‑94 (MQ=60)
                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAG                                                  <  SRR4302533.1784270/94‑1 (MQ=60)
                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAG                                                  <  SRR4302533.322696/94‑1 (MQ=60)
                                               ACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTA                                                <  SRR4302533.1994848/94‑1 (MQ=60)
                                               ACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTA                                                <  SRR4302533.651841/94‑1 (MQ=60)
                                                CTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAT                                               <  SRR4302533.3956636/94‑1 (MQ=60)
                                                 TCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATT                                              <  SRR4302533.5779569/94‑1 (MQ=60)
                                                    GCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAC                                           <  SRR4302533.3099280/94‑1 (MQ=60)
                                                     CCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACG                                          <  SRR4302533.3618894/94‑1 (MQ=60)
                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGG                                         <  SRR4302533.3904331/94‑1 (MQ=60)
                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGGTCATCGGCTTAATGCAGTATTCACGG                                         >  SRR4302533.5268052/1‑94 (MQ=60)
                                                       ATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGT                                        >  SRR4302533.4996503/1‑94 (MQ=60)
                                                         ACGCAGCGGCAGATCGCGATAAGACTTCCGCCCCTGATTGAAAATTTGTACGTGATCCGGGCAGGTCATCGGCTTAAAGCAGTATTCATGGTTC                                      >  SRR4302533.2765517/1‑94 (MQ=60)
                                                         ACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTC                                      >  SRR4302533.2765516/1‑94 (MQ=60)
                                                            CAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCA                                   <  SRR4302533.3972131/94‑1 (MQ=60)
                                                            CAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCA                                   <  SRR4302533.4849330/94‑1 (MQ=60)
                                                               CGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAA                                >  SRR4302533.4710387/1‑94 (MQ=60)
                                                                GGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAG                               <  SRR4302533.92441/94‑1 (MQ=60)
                                                                 GCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGA                              <  SRR4302533.2774675/94‑1 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                             >  SRR4302533.403251/1‑94 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                             <  SRR4302533.190985/94‑1 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                             <  SRR4302533.5112105/94‑1 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                             >  SRR4302533.3247221/1‑94 (MQ=60)
                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATG                            <  SRR4302533.2643491/94‑1 (MQ=60)
                                                                    GATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGT                           <  SRR4302533.3299082/94‑1 (MQ=60)
                                                                     ATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTG                          >  SRR4302533.3811358/1‑94 (MQ=60)
                                                                     ATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTG                          <  SRR4302533.2526802/94‑1 (MQ=60)
                                                                         CGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGA                      <  SRR4302533.2169723/94‑1 (MQ=60)
                                                                          GATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAA                     >  SRR4302533.2219079/1‑94 (MQ=60)
                                                                          GATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAA                     >  SRR4302533.6036457/1‑94 (MQ=60)
                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                    >  SRR4302533.4145882/1‑94 (MQ=60)
                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                    <  SRR4302533.3920796/94‑1 (MQ=60)
                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                    <  SRR4302533.4037336/94‑1 (MQ=60)
                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                    >  SRR4302533.5732446/1‑94 (MQ=60)
                                                                              AGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATT                 <  SRR4302533.4504927/94‑1 (MQ=60)
                                                                               GACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTG                <  SRR4302533.5895675/94‑1 (MQ=60)
                                                                                ACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGC               <  SRR4302533.1545064/94‑1 (MQ=60)
                                                                                 CTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCA              >  SRR4302533.5324011/1‑94 (MQ=60)
                                                                                  TTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCAT             <  SRR4302533.1974540/94‑1 (MQ=60)
                                                                                   TCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATC            <  SRR4302533.5226690/94‑1 (MQ=60)
                                                                                    CAGCCCCTGATTGAAAATTAGTACGTGACCCGGGCAGTTCATCGGATTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCT           <  SRR4302533.6342949/94‑1 (MQ=60)
                                                                                      GCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTT         <  SRR4302533.854549/94‑1 (MQ=60)
                                                                                      GCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTT         <  SRR4302533.3118182/94‑1 (MQ=60)
                                                                                       CCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTG        <  SRR4302533.1062467/94‑1 (MQ=60)
                                                                                         CCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA      >  SRR4302533.2470818/1‑94 (MQ=60)
                                                                                         CCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA      >  SRR4302533.2578832/1‑94 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG     >  SRR4302533.4767565/1‑94 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG     <  SRR4302533.148987/94‑1 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG     >  SRR4302533.5268292/1‑94 (MQ=60)
                                                                                            GATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT   <  SRR4302533.1782179/94‑1 (MQ=60)
                                                                                            GATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT   <  SRR4302533.3507419/94‑1 (MQ=60)
                                                                                            GANTNANAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT   <  SRR4302533.5800351/94‑1 (MQ=60)
                                                                                             ATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTG  <  SRR4302533.5212713/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTG  >  NC_000913/1799476‑1799662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑