Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,803,290 G→A intergenic (+273/+59) b1721m → / ← b1722 ankyrin repeat protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,803,2900GA100.0% 785.9 / NA 211intergenic (+273/+59)b1721m/b1722ankyrin repeat protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (106/105);  total (106/105)

Only 100 of 215 total aligned reads displayed.

CGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTC  >  NC_000913/1803201‑1803379
                                                                                         |                                                                                         
cGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGc                                                                                        <  2:6189161/93‑1 (MQ=255)
cGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGc                                                                                        <  2:4398611/93‑1 (MQ=255)
 gCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCt                                                                                       >  2:3568463/1‑93 (MQ=255)
 gCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCt                                                                                       >  1:5393602/1‑93 (MQ=255)
  cTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTg                                                                                      <  2:1076527/93‑1 (MQ=255)
  cTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTg                                                                                      >  1:3514940/1‑93 (MQ=255)
   tttGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGa                                                                                     <  1:1799409/93‑1 (MQ=255)
    ttGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAg                                                                                    >  2:4262858/1‑93 (MQ=255)
    ttGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAg                                                                                    <  1:2063424/93‑1 (MQ=255)
     tGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGc                                                                                   <  2:561370/93‑1 (MQ=255)
      gATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCa                                                                                  <  1:1252929/93‑1 (MQ=255)
      gATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCa                                                                                  >  2:3407591/1‑93 (MQ=255)
        tAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGt                                                                                <  2:2659204/93‑1 (MQ=255)
        tAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGt                                                                                <  2:228361/93‑1 (MQ=255)
          aaaaaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGa                                                                              >  2:3527693/1‑93 (MQ=255)
           aaaaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGAc                                                                             <  1:4847/93‑1 (MQ=255)
           aaaaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGAc                                                                             >  2:1122063/1‑93 (MQ=255)
           aaaaCACTGGAAGATGGTGGTGGTGCTACTCGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGAc                                                                             >  2:1629527/1‑93 (MQ=255)
            aaaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACa                                                                            >  1:2909447/1‑93 (MQ=255)
             aaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACaa                                                                           <  2:454617/93‑1 (MQ=255)
             aaCACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACaa                                                                           >  2:1535333/1‑93 (MQ=255)
                acTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACg                                                                        <  1:1239576/93‑1 (MQ=255)
                  tGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGaa                                                                      <  1:2783786/93‑1 (MQ=255)
                     aaGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGAc                                                                   >  2:4037726/1‑93 (MQ=255)
                      aGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGATGCTGAGCAGTGACAAACGAAGACa                                                                  >  2:2990823/1‑93 (MQ=255)
                      aGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACa                                                                  >  1:910164/1‑93 (MQ=255)
                        aTGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGc                                                                >  1:2418446/1‑93 (MQ=255)
                         tggtggtggTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGcc                                                               >  1:2363303/1‑93 (MQ=255)
                          ggtggtggtGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCa                                                              <  2:1386542/93‑1 (MQ=255)
                            tggtggtgCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGa                                                            <  1:6051907/93‑1 (MQ=255)
                             ggtggtgCTACTGGCAATCTGTGGCGCTATGTTATTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGAc                                                           >  1:4078004/1‑93 (MQ=255)
                               tggtgCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCg                                                         >  1:3280155/1‑93 (MQ=255)
                                  tgCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCAt                                                      >  2:1304094/1‑93 (MQ=255)
                                  tgCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCAt                                                      >  1:5013665/1‑93 (MQ=255)
                                   gCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATa                                                     >  2:4934239/1‑93 (MQ=255)
                                   gCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATa                                                     <  1:2173089/93‑1 (MQ=255)
                                    cTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATaa                                                    >  1:3564347/1‑93 (MQ=255)
                                     tACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAac                                                   >  1:1527546/1‑93 (MQ=255)
                                     tACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAac                                                   <  1:2649463/93‑1 (MQ=255)
                                      gcTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAaca                                                  <  2:547955/92‑1 (MQ=255)
                                      aCTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAaca                                                  >  1:2288914/1‑93 (MQ=255)
                                       cTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAacac                                                 <  1:4951496/93‑1 (MQ=255)
                                       cTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAacac                                                 <  2:5731292/93‑1 (MQ=255)
                                       cTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAacac                                                 <  2:1405550/93‑1 (MQ=255)
                                        tGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACg                                                >  2:2092165/1‑93 (MQ=255)
                                         ggCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACgg                                               <  1:5563669/93‑1 (MQ=255)
                                           cAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTc                                             >  1:5741843/1‑93 (MQ=255)
                                            aaTCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTcc                                            <  1:1967794/93‑1 (MQ=255)
                                            aaTCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTcc                                            >  2:5956664/1‑93 (MQ=255)
                                            aaTCTGTGGCGCTATGTTGATGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTcc                                            >  2:6083602/1‑93 (MQ=255)
                                             aTCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCg                                           <  2:5491278/93‑1 (MQ=255)
                                              tCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCgg                                          >  1:1583418/1‑93 (MQ=255)
                                              tCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCgg                                          <  2:1698434/93‑1 (MQ=255)
                                               cTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGc                                         <  1:3930444/93‑1 (MQ=255)
                                                 gtgGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCCGCAg                                       >  1:5539088/1‑93 (MQ=255)
                                                  tgGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGa                                      >  1:1843118/1‑93 (MQ=255)
                                                   ggCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAGCACGGTCCGGCAGAt                                     <  1:3655439/93‑1 (MQ=255)
                                                   ggCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGAt                                     <  1:1689471/93‑1 (MQ=255)
                                                    gcgcTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATa                                    <  1:798346/93‑1 (MQ=255)
                                                      gcTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAAt                                  >  2:5474251/1‑93 (MQ=255)
                                                        tATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAAttt                                >  2:3269833/1‑93 (MQ=255)
                                                        tATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAAttt                                <  1:4028665/93‑1 (MQ=255)
                                                         aTGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAAtttt                               <  1:122532/93‑1 (MQ=255)
                                                          tgttgtTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGCCAGCCAGACCGCATAACACGGCCCGCCAGATAATTTTa                              >  1:251830/1‑93 (MQ=255)
                                                          tgttgtTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTa                              >  2:1477725/1‑93 (MQ=255)
                                                           gttgttGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTAc                             >  1:3030404/1‑93 (MQ=255)
                                                            ttgttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACa                            <  2:3658243/93‑1 (MQ=255)
                                                            ttgttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACa                            <  2:1431040/93‑1 (MQ=255)
                                                             tgttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACAt                           <  1:1409487/93‑1 (MQ=255)
                                                              gttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATg                          >  2:2029823/1‑93 (MQ=255)
                                                              gttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACAGCATAACACGGTCCGGCAGATAATTTTACAtc                          >  1:5932353/1‑92 (MQ=255)
                                                               ttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGc                         <  1:4811617/93‑1 (MQ=255)
                                                               ttgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATGTTACATGc                         <  2:6109162/93‑1 (MQ=255)
                                                                tgCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCt                        <  2:2478691/93‑1 (MQ=255)
                                                                 gCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTg                       >  2:1035899/1‑93 (MQ=255)
                                                                  cTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGt                      <  2:530816/93‑1 (MQ=255)
                                                                  cTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGt                      <  2:2836173/93‑1 (MQ=255)
                                                                        tGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCa                <  2:5187021/93‑1 (MQ=255)
                                                                        tGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCa                <  2:2213162/93‑1 (MQ=255)
                                                                          ggCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGc              <  1:4370049/93‑1 (MQ=255)
                                                                           gcagcaATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCg             <  2:516334/93‑1 (MQ=255)
                                                                              gcaATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATa          <  1:6167188/93‑1 (MQ=255)
                                                                              gcaATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATa          >  2:467462/1‑93 (MQ=255)
                                                                               caATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATaa         <  1:4972222/93‑1 (MQ=255)
                                                                               caATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATaa         <  1:4677015/93‑1 (MQ=255)
                                                                               caATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATaa         >  1:5160025/1‑93 (MQ=255)
                                                                                aaTGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAg        >  2:3422775/1‑93 (MQ=255)
                                                                                aaTGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAg        <  2:678385/93‑1 (MQ=255)
                                                                                 aTGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAgg       >  2:4543259/1‑93 (MQ=255)
                                                                                 aTGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAgg       >  2:3615306/1‑93 (MQ=255)
                                                                                  tGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGt      <  1:3860971/93‑1 (MQ=255)
                                                                                  tGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGt      <  2:4453584/93‑1 (MQ=255)
                                                                                  tGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGt      >  1:1138739/1‑93 (MQ=255)
                                                                                    aTTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTg    >  2:2817317/1‑93 (MQ=255)
                                                                                    aTTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTg    <  2:683876/93‑1 (MQ=255)
                                                                                     tttGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGt   <  1:3045103/93‑1 (MQ=255)
                                                                                     tttGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGt   >  1:4182272/1‑93 (MQ=255)
                                                                                      ttGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTc  <  2:5586145/93‑1 (MQ=255)
                                                                                      ttGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTc  >  1:2395651/1‑93 (MQ=255)
                                                                                      ttGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTc  <  2:1250393/93‑1 (MQ=255)
                                                                                         |                                                                                         
CGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTC  >  NC_000913/1803201‑1803379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGAC  >  NC_000913/1803197‑1803383
                                                                                             |                                                                                             
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTATGTTGGGCAGCAATGATTTGA                                                                                               >  SRR4302533.1905696/1‑94 (MQ=60)
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGA                                                                                               <  SRR4302533.998759/94‑1 (MQ=60)
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGA                                                                                               >  SRR4302533.3532388/1‑94 (MQ=60)
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGA                                                                                               <  SRR4302533.1603621/94‑1 (MQ=60)
 AAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAG                                                                                              >  SRR4302533.3985563/1‑94 (MQ=60)
 AAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGCTGCTACGTTGGGCAGCAATGATTTGAG                                                                                              <  SRR4302533.5457373/94‑1 (MQ=60)
    CGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCT                                                                                           >  SRR4302533.5426006/1‑94 (MQ=60)
     GCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTG                                                                                          <  SRR4302533.3952057/94‑1 (MQ=60)
     GCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTG                                                                                          >  SRR4302533.3536605/1‑94 (MQ=60)
       tgtagTAAAAAACACTGGAAGATGGTGGTGGTGATTCTGGAACTCTGGGGCGCTATGTTTTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAG                                                                                        <  SRR4302533.1810356/89‑1 (MQ=60)
       TTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAG                                                                                        <  SRR4302533.5021672/94‑1 (MQ=60)
       TTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAG                                                                                        <  SRR4302533.1810357/94‑1 (MQ=60)
        TTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGC                                                                                       <  SRR4302533.2076144/94‑1 (MQ=60)
          GATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAG                                                                                     <  SRR4302533.1260422/94‑1 (MQ=60)
          GATAAAAAACACTGGAAGATGGTGGTAGTCCTACTGGCAATCTGTGGCGCTATGTTGTTGCTCCGTTGGGCAGCAATGATTTGAGGCTGAGCAG                                                                                     <  SRR4302533.1884328/94‑1 (MQ=60)
            TAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTG                                                                                   >  SRR4302533.3053046/1‑94 (MQ=60)
              AAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGAC                                                                                 >  SRR4302533.3824317/1‑94 (MQ=60)
              AAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGAC                                                                                 >  SRR4302533.1751701/1‑94 (MQ=60)
               AAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACA                                                                                <  SRR4302533.4893/94‑1 (MQ=60)
               AAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACA                                                                                >  SRR4302533.2927308/1‑94 (MQ=60)
                AAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAA                                                                               >  SRR4302533.5681992/1‑94 (MQ=60)
                  ACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAAC                                                                             <  SRR4302533.894592/94‑1 (MQ=60)
                    ACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGA                                                                           <  SRR4302533.1246982/94‑1 (MQ=60)
                     CTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAA                                                                          >  SRR4302533.5976376/1‑94 (MQ=60)
                      TGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAG                                                                         >  SRR4302533.4205074/1‑94 (MQ=60)
                      TGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAG                                                                         <  SRR4302533.2800887/94‑1 (MQ=60)
                       GGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGA                                                                        <  SRR4302533.1173036/94‑1 (MQ=60)
                         AAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACA                                                                      >  SRR4302533.915504/1‑94 (MQ=60)
                           GATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGC                                                                    >  SRR4302533.2433384/1‑94 (MQ=60)
                           GATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGC                                                                    >  SRR4302533.5435640/1‑94 (MQ=60)
                            ATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCC                                                                   >  SRR4302533.2733170/1‑94 (MQ=60)
                            ATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCC                                                                   <  SRR4302533.1253173/94‑1 (MQ=60)
                            ATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCC                                                                   >  SRR4302533.2377924/1‑94 (MQ=60)
                             TGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCA                                                                  >  SRR4302533.266328/1‑94 (MQ=60)
                             TGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCA                                                                  <  SRR4302533.159184/94‑1 (MQ=60)
                              GGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAG                                                                 <  SRR4302533.5142609/94‑1 (MQ=60)
                              GGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAG                                                                 <  SRR4302533.2359614/94‑1 (MQ=60)
                                TGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGAC                                                               <  SRR4302533.6088564/94‑1 (MQ=60)
                                TGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTATTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGAC                                                               >  SRR4302533.4102830/1‑94 (MQ=60)
                                 GGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACC                                                              >  SRR4302533.2861725/1‑94 (MQ=60)
                                  GTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCG                                                             >  SRR4302533.3300406/1‑94 (MQ=60)
                                   TGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGC                                                            >  SRR4302533.2218348/1‑94 (MQ=60)
                                     GTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCAT                                                          <  SRR4302533.2403787/94‑1 (MQ=60)
                                     GTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCAT                                                          >  SRR4302533.5044041/1‑94 (MQ=60)
                                       GCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAA                                                        <  SRR4302533.2186477/94‑1 (MQ=60)
                                       GCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAA                                                        >  SRR4302533.3586315/1‑94 (MQ=60)
                                        CTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAAC                                                       >  SRR4302533.1536739/1‑94 (MQ=60)
                                        CTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAAC                                                       >  SRR4302533.4074116/1‑94 (MQ=60)
                                         TACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACA                                                      <  SRR4302533.2665738/94‑1 (MQ=60)
                                         TACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACA                                                      >  SRR4302533.2303087/1‑94 (MQ=60)
                                           CTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACG                                                    >  SRR4302533.2454425/1‑94 (MQ=60)
                                           CTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACG                                                    <  SRR4302533.4981483/94‑1 (MQ=60)
                                           CTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACG                                                    >  SRR4302533.4902820/1‑94 (MQ=60)
                                           CTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACG                                                    >  SRR4302533.4329423/1‑94 (MQ=60)
                                             GGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGT                                                  <  SRR4302533.4630770/94‑1 (MQ=60)
                                             GGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGT                                                  <  SRR4302533.5597299/94‑1 (MQ=60)
                                              GCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTC                                                 >  SRR4302533.5776493/1‑94 (MQ=60)
                                                AATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCG                                               <  SRR4302533.1979822/94‑1 (MQ=60)
                                                 ATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGATGACAGCCAGACCGCATAACACGGTCCGG                                              >  SRR4302533.6096035/1‑94 (MQ=60)
                                                 ATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGG                                              >  SRR4302533.3405019/1‑94 (MQ=60)
                                                 ATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGG                                              >  SRR4302533.1592961/1‑94 (MQ=60)
                                                   CTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCA                                            <  SRR4302533.3954438/94‑1 (MQ=60)
                                                    TGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCCGCAG                                           >  SRR4302533.5572535/1‑94 (MQ=60)
                                                     GTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGA                                          >  SRR4302533.2836781/1‑94 (MQ=60)
                                                     GTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGA                                          >  SRR4302533.1854359/1‑94 (MQ=60)
                                                       GGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAGCACGGTCCGGCAGATA                                        <  SRR4302533.3677911/94‑1 (MQ=60)
                                                       GGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATA                                        <  SRR4302533.1699706/94‑1 (MQ=60)
                                                       GGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATA                                        <  SRR4302533.1245218/94‑1 (MQ=60)
                                                        GCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAA                                       <  SRR4302533.803044/94‑1 (MQ=60)
                                                         CGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAAT                                      >  SRR4302533.4747142/1‑94 (MQ=60)
                                                          GCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATT                                     >  SRR4302533.1603967/1‑94 (MQ=60)
                                                            TATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTT                                   <  SRR4302533.4053187/94‑1 (MQ=60)
                                                             ATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGCCAGCCAGACCGCATAACACGGCCCGCCAGATAATTTTA                                  >  SRR4302533.253380/1‑94 (MQ=60)
                                                             ATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTA                                  <  SRR4302533.123303/94‑1 (MQ=60)
                                                             ATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTA                                  <  SRR4302533.5588161/94‑1 (MQ=60)
                                                             ATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGGCCGGCAGATAATTTTA                                  >  SRR4302533.200328/1‑94 (MQ=60)
                                                             ATGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGGGACAAACGAAGACAGCCAGGCCGCATAACACGGTCCGGCAGATAATTTTA                                  <  SRR4302533.3139236/94‑1 (MQ=60)
                                                              TGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTAC                                 >  SRR4302533.3048949/1‑94 (MQ=60)
                                                              TGTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTAC                                 >  SRR4302533.132255/1‑94 (MQ=60)
                                                               GTTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACA                                >  SRR4302533.5781907/1‑94 (MQ=60)
                                                                TTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACAT                               <  SRR4302533.3569540/94‑1 (MQ=60)
                                                                TTGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACAT                               <  SRR4302533.4875120/94‑1 (MQ=60)
                                                                 TGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATG                              <  SRR4302533.1418008/94‑1 (MQ=60)
                                                                 TGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATG                              <  SRR4302533.725732/94‑1 (MQ=60)
                                                                 TGTTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACAGCATAACACGGTCCGGCAGATAATTTTACATC                              >  SRR4302533.5968217/1‑94 (MQ=60)
                                                                   TTGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCT                            <  SRR4302533.4840779/94‑1 (MQ=60)
                                                                    TGCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTG                           <  SRR4302533.2358942/94‑1 (MQ=60)
                                                                     GCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGT                          >  SRR4302533.730988/1‑94 (MQ=60)
                                                                     GCTACGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGT                          <  SRR4302533.1653594/94‑1 (MQ=60)
                                                                         CGTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCC                      >  SRR4302533.5000816/1‑94 (MQ=60)
                                                                          GTTGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCC                     >  SRR4302533.2079492/1‑94 (MQ=60)
                                                                            TGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAG                   >  SRR4302533.9725/1‑94 (MQ=60)
                                                                            TGGGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAG                   <  SRR4302533.4021586/94‑1 (MQ=60)
                                                                              GGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCG                 <  SRR4302533.5221543/94‑1 (MQ=60)
                                                                              GGCAGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCG                 <  SRR4302533.4396432/94‑1 (MQ=60)
                                                                                 AGCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATA              >  SRR4302533.1478756/1‑94 (MQ=60)
                                                                                  GCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAA             <  SRR4302533.2957022/94‑1 (MQ=60)
                                                                                  GCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAA             >  SRR4302533.5191167/1‑94 (MQ=60)
                                                                                  GCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAA             >  SRR4302533.5407707/1‑94 (MQ=60)
                                                                                  GCAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAA             <  SRR4302533.6204260/94‑1 (MQ=60)
                                                                                   CAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAG            >  SRR4302533.4708045/1‑94 (MQ=60)
                                                                                   CAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAG            <  SRR4302533.4705359/94‑1 (MQ=60)
                                                                                   CAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAG            <  SRR4302533.5002369/94‑1 (MQ=60)
                                                                                   CAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAG            <  SRR4302533.3602597/94‑1 (MQ=60)
                                                                                   CAATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAG            >  SRR4302533.1842856/1‑94 (MQ=60)
                                                                                    AATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGG           >  SRR4302533.3747935/1‑94 (MQ=60)
                                                                                     ATGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGT          >  SRR4302533.1145515/1‑94 (MQ=60)
                                                                                      TGATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTT         <  SRR4302533.3884539/94‑1 (MQ=60)
                                                                                        ATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGT       >  SRR4302533.2410438/1‑94 (MQ=60)
                                                                                        ATTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGT       >  SRR4302533.4207602/1‑94 (MQ=60)
                                                                                         TTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTC      <  SRR4302533.3063744/94‑1 (MQ=60)
                                                                                         TTTGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTC      >  SRR4302533.2410437/1‑94 (MQ=60)
                                                                                           TGAGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCG    <  SRR4302533.4805429/94‑1 (MQ=60)
                                                                                             AGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGAC  <  SRR4302533.26958/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGAC  >  NC_000913/1803197‑1803383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑