Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,908,347 T→C G16G (GGT→GGC)  b1828 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,908,3470TC99.6% 714.8 / NA 225G16G (GGT→GGC) b1828putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (105/119);  total (106/119)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 229 total aligned reads displayed.

AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTC  >  NC_000913/1908257‑1908438
                                                                                          |                                                                                           
aaTCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGgcgc                                                                                           <  1:5702610/93‑1 (MQ=255)
  tCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGa                                                                                         >  1:3985602/1‑93 (MQ=255)
  tCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGa                                                                                         >  1:943356/1‑93 (MQ=255)
  tCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGACTGCCATTGCCCCAGCGATACGGCGCGa                                                                                         >  2:4564895/1‑93 (MQ=255)
    aTACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGata                                                                                       <  2:6179328/93‑1 (MQ=255)
    aTACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGata                                                                                       <  1:4360744/93‑1 (MQ=255)
     tcccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                      <  2:1157747/91‑1 (MQ=255)
      aCCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAtt                                                                                     <  2:2865528/93‑1 (MQ=255)
       ccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTa                                                                                    >  1:483445/1‑93 (MQ=255)
        cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTaa                                                                                   >  1:4641343/1‑93 (MQ=255)
         ccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAc                                                                                  <  2:1239274/93‑1 (MQ=255)
          cTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAcc                                                                                 <  2:3303884/93‑1 (MQ=255)
          cTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAcc                                                                                 >  1:4792418/1‑93 (MQ=255)
           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                                >  2:2604029/1‑93 (MQ=255)
           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                                >  2:5746755/1‑93 (MQ=255)
           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                                >  1:410403/1‑93 (MQ=255)
            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                               <  2:878896/93‑1 (MQ=255)
            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                               <  1:6127717/93‑1 (MQ=255)
            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                               <  1:1033820/93‑1 (MQ=255)
            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                               >  1:192003/1‑93 (MQ=255)
             ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAtt                                                                              <  2:1124508/93‑1 (MQ=255)
             ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAtt                                                                              >  1:2239168/1‑93 (MQ=255)
              tGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtg                                                                             >  1:1146256/1‑93 (MQ=255)
               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                            <  1:189963/93‑1 (MQ=255)
               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                            >  1:4472845/1‑93 (MQ=255)
                attattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgtg                                                                           <  1:544844/93‑1 (MQ=255)
                attattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgtg                                                                           >  1:2699553/1‑93 (MQ=255)
                 ttattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGa                                                                          <  1:3772610/93‑1 (MQ=255)
                 ttattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGa                                                                          <  2:778829/93‑1 (MQ=255)
                  tattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAt                                                                         <  1:3942142/93‑1 (MQ=255)
                   attCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                        >  1:6256114/1‑93 (MQ=255)
                    ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTg                                                                       <  2:3127237/93‑1 (MQ=255)
                     tCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTgg                                                                      <  2:5805665/93‑1 (MQ=255)
                       aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTa                                                                    >  2:2364788/1‑93 (MQ=255)
                       aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTa                                                                    <  2:4316692/93‑1 (MQ=255)
                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGTCGCGATAGTAACCATTGTGATTGGTAt                                                                   >  1:6297196/1‑93 (MQ=255)
                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAt                                                                   >  2:5883194/1‑93 (MQ=255)
                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGAGATATTAACCATTGTGATTGGTAt                                                                   <  2:3818459/93‑1 (MQ=255)
                         cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAtt                                                                  <  2:351510/93‑1 (MQ=255)
                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                                 >  2:3360739/1‑93 (MQ=255)
                             gATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGa                                                              <  2:3411001/93‑1 (MQ=255)
                              atatAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAt                                                             >  2:4440255/1‑93 (MQ=255)
                               tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATg                                                            <  2:3253433/93‑1 (MQ=255)
                               tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATg                                                            >  1:4323345/1‑93 (MQ=255)
                                ataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                           >  2:5074397/1‑93 (MQ=255)
                                ataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                           >  1:3429886/1‑93 (MQ=255)
                                  aaaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGcc                                                         <  2:54905/93‑1 (MQ=255)
                                   aaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCg                                                        >  2:792299/1‑93 (MQ=255)
                                    aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGt                                                       <  1:2599548/93‑1 (MQ=255)
                                    aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGt                                                       >  2:3629436/1‑93 (MQ=255)
                                      ttCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                     <  2:59293/93‑1 (MQ=255)
                                      ttCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                     <  1:6036593/93‑1 (MQ=255)
                                      ttCCTACGCCAAAAGTTCAGGCCGTCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                     <  2:5622232/93‑1 (MQ=255)
                                       tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                    <  1:1748946/93‑1 (MQ=255)
                                       tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCt                                                    >  2:2750897/1‑93 (MQ=255)
                                         cTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTg                                                  <  1:853822/93‑1 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                 <  1:2791271/93‑1 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                 >  2:467740/1‑93 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                                 >  2:2835586/1‑93 (MQ=255)
                                           aTGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAc                                                >  1:4049935/1‑93 (MQ=255)
                                                aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcgc                                           >  2:2075010/1‑93 (MQ=255)
                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCa                                          >  1:1291114/1‑93 (MQ=255)
                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                         <  2:6016626/93‑1 (MQ=255)
                                                   aGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                        <  2:1501896/93‑1 (MQ=255)
                                                   aGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                        <  1:4887463/93‑1 (MQ=255)
                                                    gTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATc                                       <  1:922672/93‑1 (MQ=255)
                                                     ttCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCg                                      >  2:1674873/1‑93 (MQ=255)
                                                      tCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGc                                     >  2:2410109/1‑93 (MQ=255)
                                                       cAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGcc                                    <  2:1599457/93‑1 (MQ=255)
                                                         ggCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCaa                                  <  1:5955482/93‑1 (MQ=255)
                                                         ggCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCaa                                  <  1:5373618/93‑1 (MQ=255)
                                                          gCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAc                                 <  2:1895206/93‑1 (MQ=255)
                                                           ccGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACg                                <  1:5752815/93‑1 (MQ=255)
                                                           ccGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACg                                <  2:3985682/93‑1 (MQ=255)
                                                            cGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGt                               <  1:1429772/93‑1 (MQ=255)
                                                            cGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGt                               <  2:4078347/93‑1 (MQ=255)
                                                             gACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTc                              >  2:4179007/1‑93 (MQ=255)
                                                             gACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTc                              >  2:5990710/1‑93 (MQ=255)
                                                              aCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCg                             >  2:1837288/1‑93 (MQ=255)
                                                                 gCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGccc                          <  1:5205037/93‑1 (MQ=255)
                                                                  ccTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCt                         <  2:4914224/93‑1 (MQ=255)
                                                                    tGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGc                       >  1:3692482/1‑93 (MQ=255)
                                                                     gCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGcc                      <  2:2580060/93‑1 (MQ=255)
                                                                     gCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGcc                      >  1:793699/1‑93 (MQ=255)
                                                                        aTTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCACTCGCCAACGTCGCCCTGCcaac                   >  1:5813583/1‑93 (MQ=255)
                                                                        aTTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaac                   >  1:2119430/1‑93 (MQ=255)
                                                                         ttGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaaca                  >  1:2979037/1‑93 (MQ=255)
                                                                           gCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAAt                <  2:5426986/93‑1 (MQ=255)
                                                                            ccccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATc               >  1:3187677/1‑93 (MQ=255)
                                                                            ccccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATc               <  1:1514010/93‑1 (MQ=255)
                                                                             cccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCg              >  2:2098195/1‑93 (MQ=255)
                                                                             cccAGCGAGACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCg              <  2:1718639/93‑1 (MQ=255)
                                                                               cAGCGATACGGCGTGATATTACCCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCTCAATCGCCAACGTCGCCCTGCCAACAATCGcc            <  1:2488795/93‑1 (MQ=255)
                                                                                aGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCa           >  1:3608451/1‑93 (MQ=255)
                                                                                aGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCa           >  1:1495462/1‑93 (MQ=255)
                                                                                 gCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc          <  1:4593097/93‑1 (MQ=255)
                                                                                   gATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACgg        <  2:483927/93‑1 (MQ=255)
                                                                                     tACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGAc      >  1:4770689/1‑93 (MQ=255)
                                                                                       cGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCt    <  2:4054968/93‑1 (MQ=255)
                                                                                       cGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCt    <  2:1564169/93‑1 (MQ=255)
                                                                                          |                                                                                           
AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTC  >  NC_000913/1908257‑1908438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  NC_000913/1908254‑1908440
                                                                                             |                                                                                             
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGC                                                                                               <  SRR4302533.4340705/94‑1 (MQ=60)
   AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCG                                                                                            <  SRR4302533.5737050/94‑1 (MQ=60)
    ATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGA                                                                                           >  SRR4302533.948903/1‑94 (MQ=60)
    ATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGA                                                                                           >  SRR4302533.4009868/1‑94 (MQ=60)
      CATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCACCAGCGATACGGCGCGATA                                                                                         >  SRR4302533.4624646/1‑94 (MQ=60)
       ATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAT                                                                                        <  SRR4302533.4387067/94‑1 (MQ=60)
       ATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAT                                                                                        <  SRR4302533.1783454/94‑1 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      >  SRR4302533.486331/1‑94 (MQ=60)
          CCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAA                                                                                     >  SRR4302533.4669463/1‑94 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   >  SRR4302533.4821456/1‑94 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   >  SRR4302533.3031304/1‑94 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   >  SRR4302533.1979827/1‑94 (MQ=60)
             CTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCA                                                                                  >  SRR4302533.412863/1‑94 (MQ=60)
             CTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCA                                                                                  >  SRR4302533.2880609/1‑94 (MQ=60)
             CTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCA                                                                                  <  SRR4302533.1893091/94‑1 (MQ=60)
              TGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAT                                                                                 >  SRR4302533.193212/1‑94 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                <  SRR4302533.1039901/94‑1 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                <  SRR4302533.6164648/94‑1 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                >  SRR4302533.2253006/1‑94 (MQ=60)
                TTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTG                                                                               >  SRR4302533.1153097/1‑94 (MQ=60)
                 TGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGT                                                                              >  SRR4302533.4499898/1‑94 (MQ=60)
                  GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                             <  SRR4302533.191149/94‑1 (MQ=60)
                  GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                             >  SRR4302533.2716113/1‑94 (MQ=60)
                   ATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGA                                                                            <  SRR4302533.548079/94‑1 (MQ=60)
                   ATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGA                                                                            >  SRR4302533.4823328/1‑94 (MQ=60)
                    TTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAT                                                                           <  SRR4302533.3795735/94‑1 (MQ=60)
                    TTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAT                                                                           >  SRR4302533.6155687/1‑94 (MQ=60)
                     TATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATT                                                                          <  SRR4302533.3966194/94‑1 (MQ=60)
                     TATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATT                                                                          >  SRR4302533.6293525/1‑94 (MQ=60)
                     TATTCACCAAAGATATAAAATTACTATGCCAAAAGTTCAAGCCGACGGCCTGCCATTGCCCCAGCGATACCGCGCGATATTAACCAATGTGATT                                                                          >  SRR4302533.1001351/1‑94 (MQ=60)
                      ATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                         <  SRR4302533.2192546/94‑1 (MQ=60)
                       TTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGG                                                                        >  SRR4302533.5434608/1‑94 (MQ=60)
                       TTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGG                                                                        >  SRR4302533.4117480/1‑94 (MQ=60)
                         CACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTA                                                                      >  SRR4302533.6151660/1‑94 (MQ=60)
                          ACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGTCGCGATAGTAACCATTGTGATTGGTAT                                                                     >  SRR4302533.6334774/1‑94 (MQ=60)
                           CCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATT                                                                    <  SRR4302533.2490703/94‑1 (MQ=60)
                           CCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATT                                                                    >  SRR4302533.6312242/1‑94 (MQ=60)
                           CCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATT                                                                    <  SRR4302533.4603438/94‑1 (MQ=60)
                            CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                                   >  SRR4302533.5719540/1‑94 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  <  SRR4302533.940818/94‑1 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  >  SRR4302533.844393/1‑94 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  >  SRR4302533.3349816/1‑94 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  >  SRR4302533.4018003/1‑94 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  >  SRR4302533.2663130/1‑94 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302533.1000864/94‑1 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302533.19260/94‑1 (MQ=60)
                               AGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGA                                                                >  SRR4302533.4838089/1‑94 (MQ=60)
                               AGATATAAAATTCCTAGGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGA                                                                <  SRR4302533.391029/94‑1 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              <  SRR4302533.1492419/94‑1 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              >  SRR4302533.4349381/1‑94 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              <  SRR4302533.433068/94‑1 (MQ=60)
                                  TATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGG                                                             >  SRR4302533.3451075/1‑94 (MQ=60)
                                   ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                            <  SRR4302533.4552673/94‑1 (MQ=60)
                                       AATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTC                                                        <  SRR4302533.2615523/94‑1 (MQ=60)
                                        ATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCC                                                       <  SRR4302533.1509382/94‑1 (MQ=60)
                                         TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                      <  SRR4302533.1085513/94‑1 (MQ=60)
                                         TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                      <  SRR4302533.6073175/94‑1 (MQ=60)
                                          TCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                     <  SRR4302533.1759594/94‑1 (MQ=60)
                                          TCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                     <  SRR4302533.581122/94‑1 (MQ=60)
                                          TCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                     >  SRR4302533.4323183/1‑94 (MQ=60)
                                           CCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTG                                                    <  SRR4302533.6217835/94‑1 (MQ=60)
                                           CCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTG                                                    >  SRR4302533.5343681/1‑94 (MQ=60)
                                            CTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGA                                                   >  SRR4302533.4889971/1‑94 (MQ=60)
                                            CTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGA                                                   <  SRR4302533.858825/94‑1 (MQ=60)
                                             TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                                  <  SRR4302533.2808440/94‑1 (MQ=60)
                                             TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                                  >  SRR4302533.4074604/1‑94 (MQ=60)
                                                 CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                              <  SRR4302533.124441/94‑1 (MQ=60)
                                                  CAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTTGTATTTCGATGGCCGTCCTTGACGGCGC                                             >  SRR4302533.717717/1‑94 (MQ=60)
                                                  CAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGC                                             >  SRR4302533.144991/1‑94 (MQ=60)
                                                   AAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCA                                            >  SRR4302533.1298894/1‑94 (MQ=60)
                                                      AGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATC                                         <  SRR4302533.4917076/94‑1 (MQ=60)
                                                      AGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATC                                         >  SRR4302533.1411528/1‑94 (MQ=60)
                                                       GTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCG                                        <  SRR4302533.928093/94‑1 (MQ=60)
                                                        TTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGC                                       >  SRR4302533.4775759/1‑94 (MQ=60)
                                                        TTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGC                                       >  SRR4302533.859183/1‑94 (MQ=60)
                                                           AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTCTTTTGATGGTCGTCCTTGAAGGCCCAATCGCCAC                                    >  SRR4302533.681236/1‑94 (MQ=60)
                                                            GGCCGACGGCCTGCCATTGCCCCAGTGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAC                                   <  SRR4302533.5776141/94‑1 (MQ=60)
                                                            GGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAC                                   <  SRR4302533.5991509/94‑1 (MQ=60)
                                                            GGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAC                                   <  SRR4302533.5991508/94‑1 (MQ=60)
                                                            GGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAC                                   <  SRR4302533.5405908/94‑1 (MQ=60)
                                                              CCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGT                                 <  SRR4302533.5787524/94‑1 (MQ=60)
                                                               CGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTC                                <  SRR4302533.1618209/94‑1 (MQ=60)
                                                               CGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTC                                <  SRR4302533.1438414/94‑1 (MQ=60)
                                                               CGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTC                                >  SRR4302533.5147998/1‑94 (MQ=60)
                                                                GACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCG                               >  SRR4302533.5745146/1‑94 (MQ=60)
                                                                   GGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCC                            <  SRR4302533.3705715/94‑1 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           <  SRR4302533.5236402/94‑1 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           <  SRR4302533.2458031/94‑1 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           <  SRR4302533.4362340/94‑1 (MQ=60)
                                                                      CTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGC                         >  SRR4302533.3715168/1‑94 (MQ=60)
                                                                       TGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCC                        >  SRR4302533.798370/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCACTCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.5848695/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.2560344/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.5291883/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.2132504/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.4485766/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302533.3907038/1‑94 (MQ=60)
                                                                           ATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACA                    >  SRR4302533.2997267/1‑94 (MQ=60)
                                                                             TGCCCCAGCGATACGGCGCTATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAAT                  <  SRR4302533.3553468/94‑1 (MQ=60)
                                                                              GCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATC                 >  SRR4302533.3207288/1‑94 (MQ=60)
                                                                               CCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCG                <  SRR4302533.1523133/94‑1 (MQ=60)
                                                                               CCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCG                <  SRR4302533.4801632/94‑1 (MQ=60)
                                                                                CCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGC               >  SRR4302533.3968863/1‑94 (MQ=60)
                                                                                 CCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCC              <  SRR4302533.273448/94‑1 (MQ=60)
                                                                                 CCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCC              >  SRR4302533.5894940/1‑94 (MQ=60)
                                                                                  CAGCGATACGGCGTGATATTACCCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCTCAATCGCCAACGTCGCCCTGCCAACAATCGCCA             <  SRR4302533.2504123/94‑1 (MQ=60)
                                                                                  CAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCA             >  SRR4302533.3630664/1‑94 (MQ=60)
                                                                                  CAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCA             >  SRR4302533.1504473/1‑94 (MQ=60)
                                                                                   AGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAC            >  SRR4302533.3741184/1‑94 (MQ=60)
                                                                                    GCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACG           <  SRR4302533.4620919/94‑1 (MQ=60)
                                                                                     CGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGG          >  SRR4302533.2753011/1‑94 (MQ=60)
                                                                                       ATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGAC        <  SRR4302533.353091/94‑1 (MQ=60)
                                                                                       ATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGAC        >  SRR4302533.4799604/1‑94 (MQ=60)
                                                                                           GGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTC    <  SRR4302533.4155886/94‑1 (MQ=60)
                                                                                           GGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTC    >  SRR4302533.3566009/1‑94 (MQ=60)
                                                                                            GCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCA   <  SRR4302533.3722687/94‑1 (MQ=60)
                                                                                            GCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCA   <  SRR4302533.5098820/94‑1 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  SRR4302533.4279475/1‑94 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  <  SRR4302533.5561134/94‑1 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  <  SRR4302533.1964546/94‑1 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  SRR4302533.3747443/1‑94 (MQ=60)
                                                                                             |                                                                                             
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  NC_000913/1908254‑1908440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑