Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,973,518	A→T	D254E (GAT→GAA)	motB ←	MotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,973,518	0	A	T	100.0%	666.5 / NA	181	D254E (GAT→GAA)	motB	MotB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (81/100); total (81/100)

Only 100 of 205 total aligned reads displayed.

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

tGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttc                                                                                         >  1:4482917/1‑93 (MQ=255)

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttca                                                                                        <  1:1227155/93‑1 (MQ=255)

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttca                                                                                        <  1:616284/93‑1 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                      <  2:5772566/93‑1 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                      <  1:2828937/93‑1 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                      >  1:1910202/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  <  1:366174/93‑1 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  >  1:3437957/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  >  1:3423444/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                  >  1:5273205/1‑93 (MQ=255)

         cTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTcc                                                                                <  2:2917785/93‑1 (MQ=255)

          tGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcg                                                                               >  1:2223914/1‑93 (MQ=255)

             cTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGa                                                                            >  2:5678721/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCCGGTCCGCGAt                                                                           >  2:4720627/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                           <  2:198625/93‑1 (MQ=255)

               ttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATc                                                                          >  2:2768783/1‑93 (MQ=255)

                 cGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGc                                                                        <  2:5399118/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                       <  1:4713951/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                       <  1:2940826/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                       >  2:510100/1‑93 (MQ=255)

                   gCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCtt                                                                      >  1:1650488/1‑93 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                     >  2:4403861/1‑93 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                     >  1:1604603/1‑93 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                   >  1:2410229/1‑93 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgc                                                                  <  1:5478671/93‑1 (MQ=255)

                       tttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgc                                                                  <  1:5973789/93‑1 (MQ=255)

                        ttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcg                                                                 >  1:3124247/1‑93 (MQ=255)

                        ttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcg                                                                 >  1:4923675/1‑93 (MQ=255)

                         tCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAgcgc                                                                <  1:4875662/93‑1 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                              >  2:4816664/1‑93 (MQ=255)

                            tGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATc                                                             >  2:3852611/1‑93 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGGTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTTCGCGATCGCTTAAACGCAtact                                                           >  2:1682651/1‑90 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          >  1:3903387/1‑93 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          <  1:1311076/93‑1 (MQ=255)

                               aaaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                          >  1:1815484/1‑93 (MQ=255)

                                aaaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTg                                                         <  1:4394165/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        <  1:1103634/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        <  1:99011/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        <  1:5130530/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        <  2:5168788/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                        >  2:6085670/1‑93 (MQ=255)

                                      ccTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCa                                                   >  2:3821589/1‑93 (MQ=255)

                                      ccTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCa                                                   <  1:4773772/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                  >  2:4497342/1‑93 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                  <  1:2439884/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAACCTTGCCGCCAt                                                  >  2:1647321/1‑93 (MQ=255)

                                        tGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATg                                                 >  2:150287/1‑93 (MQ=255)

                                          ttCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGcc                                               <  2:3129237/93‑1 (MQ=255)

                                          ttCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGcc                                               <  2:2736805/93‑1 (MQ=255)

                                          ttCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGcc                                               >  2:2452863/1‑93 (MQ=255)

                                           tCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcg                                              >  2:6041959/1‑93 (MQ=255)

                                           tCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcg                                              >  2:1044828/1‑93 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  2:5515965/93‑1 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                             <  1:4317272/93‑1 (MQ=255)

                                             ggCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgac                                            >  2:6214338/1‑93 (MQ=255)

                                              gCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACAGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacg                                           <  1:1711421/93‑1 (MQ=255)

                                               cTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCACGCcgacga                                          >  2:2952701/1‑93 (MQ=255)

                                                  gtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGacac                                       >  2:2214490/1‑93 (MQ=255)

                                                   tttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACg                                      <  2:3932637/93‑1 (MQ=255)

                                                   tttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACg                                      >  2:4042274/1‑93 (MQ=255)

                                                      gttCAGGACAGGCAGGCTGATGCGACGGTTGACGTCTTCATCAGTTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACTCGTaa                                   <  2:4576527/93‑1 (MQ=255)

                                                      gttCAGCACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTaa                                   <  2:3592950/93‑1 (MQ=255)

                                                       ttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAac                                  <  1:5026911/93‑1 (MQ=255)

                                                        tCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAaca                                 >  2:4193209/1‑93 (MQ=255)

                                                          aGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACt                               <  2:5262097/93‑1 (MQ=255)

                                                            tACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACttt                             <  2:5498977/93‑1 (MQ=255)

                                                             aCCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTg                            <  2:3699895/93‑1 (MQ=255)

                                                              ccaccaGGCTGACGCGAGGGTTGACGGCGTCATCAGCTCCGCGATCGCTTAAGCGCATCGTTGCCGCGATGCCGACGACACGTAACACTTTGc                           <  1:2568414/93‑1 (MQ=255)

                                                              ccAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGc                           <  1:5819480/93‑1 (MQ=255)

                                                              ccAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGc                           <  2:1290663/93‑1 (MQ=255)

                                                               cagcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGcc                          <  2:762460/93‑1 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        <  2:1363591/93‑1 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        >  2:2607783/1‑93 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                        >  2:139426/1‑93 (MQ=255)

                                                                   agGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTa                      <  1:5678548/93‑1 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                     <  1:1052915/93‑1 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                     >  1:6058869/1‑93 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGACGCTAt                     >  2:4346580/1‑93 (MQ=255)

                                                                     gCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATc                    >  1:2071212/1‑93 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                   <  1:4146342/93‑1 (MQ=255)

                                                                       tGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCa                  >  1:274486/1‑93 (MQ=255)

                                                                        gATTCGACCGTTGAAGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCaa                 <  2:6002343/93‑1 (MQ=255)

                                                                        gATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCCTGCCGACGACACGTAACACTTTGCCGCTATCCaa                 >  2:2562746/1‑93 (MQ=255)

                                                                         aTGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAc                >  1:2682963/1‑93 (MQ=255)

                                                                         aTGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAc                <  2:2173312/93‑1 (MQ=255)

                                                                           gCGACGGTTGACGGCTTCATCAGGCCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAccc              >  1:3861308/1‑93 (MQ=255)

                                                                            cGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCt             >  2:5847899/1‑93 (MQ=255)

                                                                             gACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTc            <  1:2518404/93‑1 (MQ=255)

                                                                              aCGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTcc           >  2:1907128/1‑93 (MQ=255)

                                                                              aCGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTcc           <  2:2053566/93‑1 (MQ=255)

                                                                              aCGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTcc           >  2:4135659/1‑93 (MQ=255)

                                                                               cGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCg          <  1:2172772/93‑1 (MQ=255)

                                                                                ggTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGa         >  2:3381590/1‑93 (MQ=255)

                                                                                 gTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAc        <  2:4836539/93‑1 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc       <  1:350300/93‑1 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc       >  2:5359329/1‑93 (MQ=255)

                                                                                    gACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCAt     <  2:1692010/93‑1 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc    <  2:3265488/93‑1 (MQ=255)

                                                                                       ggCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAg  <  1:767277/93‑1 (MQ=255)

                                                                                       ggCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAg  <  2:1549719/93‑1 (MQ=255)

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG  >  NC_000913/1973428‑1973607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973427‑1973611

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             >  SRR4302533.4510065/1‑94 (MQ=60)

  GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           >  SRR4302533.1532138/1‑94 (MQ=60)

  GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302533.619904/94‑1 (MQ=60)

  GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302533.1234504/94‑1 (MQ=60)

  GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302533.619903/94‑1 (MQ=60)

   GCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATC                                                                                          >  SRR4302533.1921856/1‑94 (MQ=60)

   GCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATC                                                                                          <  SRR4302533.4429580/94‑1 (MQ=60)

    CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGTCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         <  SRR4302533.3936284/94‑1 (MQ=60)

    CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         >  SRR4302533.231401/1‑94 (MQ=60)

    CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         <  SRR4302533.2846342/94‑1 (MQ=60)

     TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        <  SRR4302533.532526/94‑1 (MQ=60)

     TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        <  SRR4302533.5946915/94‑1 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302533.5304824/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302533.3459190/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302533.3444593/1‑94 (MQ=60)

        TTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTC                                                                                     <  SRR4302533.368391/94‑1 (MQ=60)

          CTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCG                                                                                   >  SRR4302533.2237648/1‑94 (MQ=60)

          CTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCG                                                                                   >  SRR4302533.4187931/1‑94 (MQ=60)

              GTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAT                                                                               <  SRR4302533.4915035/94‑1 (MQ=60)

               TTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATC                                                                              <  SRR4302533.4880670/94‑1 (MQ=60)

                TTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCG                                                                             <  SRR4302533.181675/94‑1 (MQ=60)

                   GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTT                                                                          <  SRR4302533.4749730/94‑1 (MQ=60)

                   GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTT                                                                          <  SRR4302533.2958788/94‑1 (MQ=60)

                   GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTT                                                                          <  SRR4302533.4742521/94‑1 (MQ=60)

                   GGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTT                                                                          >  SRR4302533.1660487/1‑94 (MQ=60)

                    GCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTA                                                                         >  SRR4302533.1614316/1‑94 (MQ=60)

                      GTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAG                                                                       >  SRR4302533.2425115/1‑94 (MQ=60)

                      GTTTTCATGCAAAATGGCCTGGTCGTCTTTTTGGTTTAGTTCCTGCAGGCTGATGCGTCGGTTGACGGCCTCATCTGGTCTGCGATCGCTTAAG                                                                       >  SRR4302533.6340785/1‑94 (MQ=32)

                        TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     >  SRR4302533.3143452/1‑94 (MQ=60)

                        TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     >  SRR4302533.4953503/1‑94 (MQ=60)

                        TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     <  SRR4302533.5511699/94‑1 (MQ=60)

                        TTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCG                                                                     <  SRR4302533.6009949/94‑1 (MQ=60)

                          TCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCTCTTAAGCGCA                                                                   <  SRR4302533.1637035/94‑1 (MQ=60)

                          TCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCA                                                                   <  SRR4302533.3461478/94‑1 (MQ=60)

                          TCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCA                                                                   <  SRR4302533.4905201/94‑1 (MQ=60)

                           CATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAT                                                                  <  SRR4302533.3906655/94‑1 (MQ=60)

                               CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              >  SRR4302533.1826568/1‑94 (MQ=60)

                               CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              >  SRR4302533.2853925/1‑94 (MQ=60)

                               CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              >  SRR4302533.3927227/1‑94 (MQ=60)

                                AAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTG                                                             <  SRR4302533.1318946/94‑1 (MQ=60)

                                 AAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGC                                                            <  SRR4302533.4420694/94‑1 (MQ=60)

                                 AAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGC                                                            <  SRR4302533.1671258/94‑1 (MQ=60)

                                 AAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGC                                                            <  SRR4302533.5750944/94‑1 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302533.1110168/94‑1 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302533.5161522/94‑1 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302533.99626/94‑1 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAACGTTGCC                                                           <  SRR4302533.4369936/94‑1 (MQ=60)

                                   ATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCG                                                          <  SRR4302533.3093335/94‑1 (MQ=60)

                                       CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAT                                                      <  SRR4302533.4802698/94‑1 (MQ=60)

                                       CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAT                                                      <  SRR4302533.1887360/94‑1 (MQ=60)

                                        CTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATG                                                     <  SRR4302533.2454952/94‑1 (MQ=60)

                                          GTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCC                                                   >  SRR4302533.2208114/1‑94 (MQ=60)

                                            TCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGA                                                 <  SRR4302533.2051736/94‑1 (MQ=60)

                                             CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302533.4343270/94‑1 (MQ=60)

                                             CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302533.2535676/94‑1 (MQ=60)

                                              GTCTTGTGTGTGCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               <  SRR4302533.5891533/94‑1 (MQ=60)

                                               GCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGA                                              >  SRR4302533.2450628/1‑94 (MQ=60)

                                               GCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGA                                              <  SRR4302533.2746266/94‑1 (MQ=60)

                                               GCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACAGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGA                                              <  SRR4302533.1721792/94‑1 (MQ=60)

                                                  TGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACAC                                           >  SRR4302533.1315808/1‑94 (MQ=60)

                                                   GTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACG                                          >  SRR4302533.2809053/1‑94 (MQ=60)

                                                     TTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTA                                        >  SRR4302533.6139854/1‑94 (MQ=60)

                                                     TTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCATTGCCGCCATGCCGACGACACGTA                                        >  SRR4302533.106473/1‑94 (MQ=60)

                                                        TTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACA                                     <  SRR4302533.5057351/94‑1 (MQ=60)

                                                            GTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTT                                 <  SRR4302533.34558/94‑1 (MQ=60)

                                                             TACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTG                                >  SRR4302533.3869487/1‑94 (MQ=60)

                                                             TACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTG                                >  SRR4302533.498526/1‑94 (MQ=60)

                                                             TACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTG                                <  SRR4302533.2497858/94‑1 (MQ=60)

                                                              ACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGC                               >  SRR4302533.2659383/1‑94 (MQ=60)

                                                              ACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGC                               >  SRR4302533.5773747/1‑94 (MQ=60)

                                                               CCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGCCGACACGTAACACTTTGCC                              >  SRR4302533.2180195/1‑94 (MQ=60)

                                                               CCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCC                              <  SRR4302533.5854633/94‑1 (MQ=60)

                                                               CCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCC                              <  SRR4302533.5532303/94‑1 (MQ=60)

                                                               CCACCAGGCTGACGCGAGGGTTGACGGCGTCATCAGCTCCGCGATCGCTTAAGCGCATCGTTGCCGCGATGCCGACGACACGTAACACTTTGCC                              <  SRR4302533.2584218/94‑1 (MQ=60)

                                                                CAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCG                             <  SRR4302533.5078747/94‑1 (MQ=60)

                                                                 AGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGC                            >  SRR4302533.4595681/1‑94 (MQ=60)

                                                                 AGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGC                            >  SRR4302533.6058365/1‑94 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         <  SRR4302533.5712845/94‑1 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302533.363356/1‑94 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302533.6095550/1‑94 (MQ=60)

                                                                     GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        <  SRR4302533.1059117/94‑1 (MQ=60)

                                                                     GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        >  SRR4302533.2083970/1‑94 (MQ=60)

                                                                      GCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCC                       <  SRR4302533.1187680/94‑1 (MQ=60)

                                                                       CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      <  SRR4302533.3286573/94‑1 (MQ=60)

                                                                       CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      <  SRR4302533.4171467/94‑1 (MQ=60)

                                                                       CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      >  SRR4302533.276157/1‑94 (MQ=60)

                                                                         GATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAC                    >  SRR4302533.2699400/1‑94 (MQ=60)

                                                                           TGCGACGGTTGACGGCTTCATCAGGCCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCC                  >  SRR4302533.3884878/1‑94 (MQ=60)

                                                                              GACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCC               >  SRR4302533.3045725/1‑94 (MQ=60)

                                                                              GACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCC               <  SRR4302533.2533919/94‑1 (MQ=60)

                                                                                CGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGA             <  SRR4302533.2186132/94‑1 (MQ=60)

                                                                                 GGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAC            >  SRR4302533.5235607/1‑94 (MQ=60)

                                                                                   TTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCA          <  SRR4302533.352434/94‑1 (MQ=60)

                                                                                       CGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAG      <  SRR4302533.877266/94‑1 (MQ=60)

                                                                                        GGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGT     >  SRR4302533.1232779/1‑94 (MQ=60)

                                                                                        GGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGT     <  SRR4302533.771803/94‑1 (MQ=60)

                                                                                          CTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTC   <  SRR4302533.3118177/94‑1 (MQ=60)

                                                                                          CTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTC   <  SRR4302533.2033012/94‑1 (MQ=60)

                                                                                           TTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  <  SRR4302533.2809020/94‑1 (MQ=60)

                                                                                           TTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  SRR4302533.2901071/1‑94 (MQ=60)

                                                                                           TTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  <  SRR4302533.1310553/94‑1 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973427‑1973611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`