Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,983,127	(C)_5→6	intergenic (‑35/+35)	araG ← / ← araF	ATP‑binding component of high‑affinity L‑arabinose transport system/L‑arabinose‑binding periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,983,122	1	.	C	98.6%	651.6 / 1.5	207	intergenic (‑30/+40)	araG/araF	ATP‑binding component of high‑affinity L‑arabinose transport system/L‑arabinose‑binding periplasmic protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (97/107); total (98/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 237 total aligned reads displayed.

CTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTC  >  NC_000913/1983030‑1983209

cTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTca‑c‑a                                                                                          >  1:3750520/1‑93 (MQ=255)

 ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCa‑c‑a‑c                                                                                        >  1:3859270/1‑93 (MQ=255)

 ttAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCa‑c‑a‑c                                                                                        >  2:292044/1‑93 (MQ=255)

 ttAACGCCGGGAAACGTTTTACCGATACCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCa‑c‑a‑c                                                                                        >  1:2147028/1‑93 (MQ=255)

  tAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑cc                                                                                       <  1:4275676/93‑1 (MQ=255)

   aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑ccc                                                                                      >  1:123434/1‑93 (MQ=255)

   aaCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑ccc                                                                                      >  1:735450/1‑93 (MQ=255)

    aCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑cccc                                                                                     >  1:4017154/1‑93 (MQ=255)

        cGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑cccccctc                                                                                 <  2:1814624/93‑4 (MQ=255)

           gAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGc                                                                               >  2:1658264/1‑93 (MQ=255)

             aaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGcat                                                                             >  2:4262249/1‑93 (MQ=255)

                gTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGcatcat                                                                          >  2:3229612/1‑93 (MQ=255)

                 ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATg                                                                         >  2:6073522/1‑93 (MQ=255)

                 ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATg                                                                         <  1:1586131/93‑1 (MQ=255)

                 ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATg                                                                         >  2:1378657/1‑93 (MQ=255)

                  tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGc                                                                        <  1:1088733/93‑1 (MQ=255)

                   ttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCa                                                                       <  1:3172158/93‑1 (MQ=255)

                      ccGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCagag                                                                    <  1:4756557/93‑1 (MQ=255)

                      ccGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCagag                                                                    <  2:5053775/93‑1 (MQ=255)

                       cGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAgg                                                                   <  2:4975850/93‑1 (MQ=255)

                         aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAgggg                                                                 >  1:5081566/1‑93 (MQ=255)

                         aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAgggg                                                                 <  1:5265159/93‑1 (MQ=255)

                          tGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGa                                                                >  2:2086781/1‑93 (MQ=255)

                          tGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGa                                                                >  1:3443593/1‑93 (MQ=255)

                           gCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGaa                                                               <  2:4180574/93‑1 (MQ=255)

                             cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAAtt                                                             >  1:3565330/1‑93 (MQ=255)

                             cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAAtt                                                             >  1:488557/1‑93 (MQ=255)

                                 aaaTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTc                                                         >  2:1763322/1‑93 (MQ=255)

                                 aaaTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTc                                                         <  1:5959484/93‑1 (MQ=255)

                                     gagagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGc                                                     <  1:198658/93‑1 (MQ=255)

                                     aagagaTACGGGGGAGACTGTTCCATACCGTCTCCGTGACTCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGc                                                     <  2:5439311/92‑1 (MQ=255)

                                      agagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCa                                                    >  1:1412079/1‑93 (MQ=255)

                                        agaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCaaa                                                  <  2:3509084/93‑1 (MQ=255)

                                           tgcGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAtta                                               <  2:1328321/91‑1 (MQ=255)

                                           tACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAtta                                               >  1:4149369/1‑93 (MQ=255)

                                           tACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAtta                                               >  1:1384302/1‑93 (MQ=255)

                                            aCGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttac                                              <  1:1537086/93‑1 (MQ=255)

                                            aCGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttac                                              <  2:4777917/93‑1 (MQ=255)

                                            aCGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttac                                              >  2:1888692/1‑93 (MQ=255)

                                             cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttact                                             <  1:2805825/93‑1 (MQ=255)

                                             cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttact                                             <  2:2541813/93‑1 (MQ=255)

                                             cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttact                                             <  1:6119431/93‑1 (MQ=255)

                                              ggggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactt                                            <  1:1251859/93‑1 (MQ=255)

                                              ggggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactt                                            >  2:3660524/1‑93 (MQ=255)

                                              ggggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactt                                            <  1:5569590/93‑1 (MQ=255)

                                               gggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑CGC‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactta                                           <  2:3841682/93‑1 (MQ=255)

                                                ggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttacttac                                          >  2:900135/1‑93 (MQ=255)

                                                ggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttacttac                                          <  1:2365383/93‑1 (MQ=255)

                                                ggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttacttac                                          <  1:2426658/93‑1 (MQ=255)

                                                 gTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAcc                                         >  2:2697369/1‑93 (MQ=255)

                                                 gTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAcc                                         <  2:4411863/93‑1 (MQ=255)

                                                 gTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAcc                                         <  1:4854124/93‑1 (MQ=255)

                                                 gTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAcc                                         <  2:4635008/93‑1 (MQ=255)

                                                  tAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAccc                                        >  2:229634/1‑92 (MQ=255)

                                                   aGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGc                                       <  1:3953801/93‑1 (MQ=255)

                                                    gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGcc                                      <  2:2166045/93‑1 (MQ=255)

                                                    gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGcc                                      <  1:836368/93‑1 (MQ=255)

                                                    gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGcc                                      >  2:6218334/1‑93 (MQ=255)

                                                     aCTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTTCGGCAAATTACTTACCCCCt                                     >  1:4396173/1‑93 (MQ=255)

                                                      cTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTa                                    <  2:1933290/93‑1 (MQ=255)

                                                       tGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTaa                                   >  2:5983139/1‑93 (MQ=255)

                                                        gTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTaaa                                  <  2:4656638/93‑1 (MQ=255)

                                                        gTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTaaa                                  >  1:3351110/1‑93 (MQ=255)

                                                          tGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAcc                                <  1:2604789/93‑1 (MQ=255)

                                                             aTAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                             >  1:2689384/1‑93 (MQ=255)

                                                              tatcGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCTGGCAAATTACTTACCGCCTAAACCtttt                            <  2:5868452/90‑1 (MQ=255)

                                                              tAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCCTGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                            >  1:2333401/1‑93 (MQ=255)

                                                              tAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                            >  1:3330041/1‑93 (MQ=255)

                                                               aaCGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttttt                           <  1:2876587/93‑1 (MQ=255)

                                                               aaCGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttttt                           <  2:5039554/93‑1 (MQ=255)

                                                               aaCGTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttttt                           <  1:428362/93‑1 (MQ=255)

                                                                  gTCTCCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtct                        >  1:1659660/1‑93 (MQ=255)

                                                                   tctcCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                       >  2:3024776/1‑93 (MQ=255)

                                                                    ctcCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                      <  2:6153100/93‑1 (MQ=255)

                                                                     tcCGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCa                     <  1:5369169/93‑1 (MQ=255)

                                                                      ccGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAg                    <  2:155396/93‑1 (MQ=255)

                                                                      ccGTGAATCACTGGTCGTTCA‑A‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAg                    <  2:4221156/93‑1 (MQ=255)

                                                                       cGTGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGt                   <  1:1596622/93‑1 (MQ=255)

                                                                         tGAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttc                 >  2:2815990/1‑93 (MQ=255)

                                                                          gAATCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttct                >  1:36364/1‑93 (MQ=255)

                                                                           aaTCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttctt               <  2:5213409/93‑1 (MQ=255)

                                                                             tCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTtcttct             >  2:5768322/1‑93 (MQ=255)

                                                                             tCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTtcttct             >  1:4893882/1‑93 (MQ=255)

                                                                             tCACTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTtcttct             <  1:5638663/93‑1 (MQ=255)

                                                                               aCTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCttt           >  2:5171713/1‑93 (MQ=255)

                                                                                cTGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTa          >  1:133200/1‑93 (MQ=255)

                                                                                 tGGTCGTTCACC‑C‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaa         >  2:3072394/1‑93 (MQ=255)

                                                                                 tGGTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaa         >  1:6161103/1‑93 (MQ=255)

                                                                                  ggTCGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa        <  2:3708402/93‑1 (MQ=255)

                                                                                     cGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtt     <  1:292044/93‑1 (MQ=255)

                                                                                     cGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtt     <  2:2517971/93‑1 (MQ=255)

                                                                                     cGTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTCACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtt     <  1:4551871/93‑1 (MQ=255)

                                                                                      gTTCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTg    <  1:6137015/93‑1 (MQ=255)

                                                                                       ttCA‑C‑ACCCCCCTCTGCATCATGCAGAGTGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt   <  2:1043609/93‑1 (MQ=255)

                                                                                       ttCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGGATTTTTTCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCCGTTCTTCTTTTAAAtttt   >  2:4292633/1‑91 (MQ=255)

                                                                                       ttCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt   <  1:5225213/93‑1 (MQ=255)

                                                                                       ttCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt   <  1:5895322/93‑1 (MQ=255)

                                                                                        tCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc  <  1:1897536/93‑1 (MQ=255)

                                                                                        tCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc  >  1:1787227/1‑93 (MQ=255)

                                                                                        tCA‑C‑ACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc  <  2:3201309/93‑1 (MQ=255)

CTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCA‑C‑A‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTC  >  NC_000913/1983030‑1983209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACA‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCA  >  NC_000913/1983034‑1983220

ACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACA‑CCCCC                                                                                               >  SRR4302533.510546/1‑94 (MQ=60)

 CGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACA‑CCCCCC                                                                                              <  SRR4302533.1832706/94‑1 (MQ=60)

    CGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCT                                                                                            <  SRR4302533.3901895/94‑1 (MQ=60)

     GGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACAGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTG                                                                                           >  SRR4302533.4112680/1‑94 (MQ=60)

       GAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCA                                                                                         >  SRR4302533.2480311/1‑94 (MQ=60)

        AAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCAT                                                                                        >  SRR4302533.790408/1‑94 (MQ=60)

           CGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCAT                                                                                     >  SRR4302533.402055/1‑94 (MQ=60)

           CGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCAT                                                                                     <  SRR4302533.4158805/94‑1 (MQ=60)

           CGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCAT                                                                                     >  SRR4302533.2798832/1‑94 (MQ=60)

            GTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATG                                                                                    >  SRR4302533.161902/1‑94 (MQ=60)

             TTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGC                                                                                   <  SRR4302533.1595692/94‑1 (MQ=60)

              TTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCA                                                                                  <  SRR4302533.1095161/94‑1 (MQ=60)

              TTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCA                                                                                  <  SRR4302533.68156/94‑1 (MQ=60)

               ttcccgaatCCGCGAAATGAGAGATACGGGCTAGACTGTTTCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAG                                                                                 <  SRR4302533.4854877/85‑1 (MQ=60)

               TTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAG                                                                                 <  SRR4302533.3191686/94‑1 (MQ=60)

               TTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAG                                                                                 >  SRR4302533.4570879/1‑94 (MQ=60)

               TTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAG                                                                                 <  SRR4302533.4854876/94‑1 (MQ=60)

                TACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGA                                                                                <  SRR4302533.3366672/94‑1 (MQ=60)

                 ACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAG                                                                               <  SRR4302533.3828264/94‑1 (MQ=60)

                 ACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAG                                                                               >  SRR4302533.4923897/1‑94 (MQ=60)

                  CCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGG                                                                              <  SRR4302533.309516/94‑1 (MQ=60)

                  CCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGG                                                                              <  SRR4302533.2305255/94‑1 (MQ=60)

                  CCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGG                                                                              <  SRR4302533.5751946/94‑1 (MQ=60)

                  CCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGG                                                                              <  SRR4302533.4785420/94‑1 (MQ=60)

                   CGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGG                                                                             <  SRR4302533.2626973/94‑1 (MQ=60)

                   CGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGG                                                                             <  SRR4302533.156598/94‑1 (MQ=60)

                   CGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGG                                                                             >  SRR4302533.5614607/1‑94 (MQ=60)

                    GATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGG                                                                            >  SRR4302533.5112278/1‑94 (MQ=60)

                    GATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGG                                                                            >  SRR4302533.1297708/1‑94 (MQ=60)

                     ATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGA                                                                           <  SRR4302533.5296756/94‑1 (MQ=60)

                     ATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGA                                                                           >  SRR4302533.3464862/1‑94 (MQ=60)

                     ATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGA                                                                           >  SRR4302533.5649067/1‑94 (MQ=60)

                        CCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCCGAGGGGAATT                                                                        >  SRR4302533.5380028/1‑94 (MQ=60)

                        CCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATT                                                                        >  SRR4302533.491480/1‑94 (MQ=60)

                        CCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATT                                                                        >  SRR4302533.3587303/1‑94 (MQ=60)

                           CGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTT                                                                     <  SRR4302533.3596983/94‑1 (MQ=60)

                             AAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCC                                                                   <  SRR4302533.5995533/94‑1 (MQ=60)

                             AAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCC                                                                   >  SRR4302533.1338280/1‑94 (MQ=60)

                             AAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCC                                                                   >  SRR4302533.3209735/1‑94 (MQ=60)

                             AAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCC                                                                   <  SRR4302533.1601627/94‑1 (MQ=60)

                             AAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCC                                                                   >  SRR4302533.4883804/1‑94 (MQ=60)

                              AATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCG                                                                  <  SRR4302533.3440213/94‑1 (MQ=60)

                                TGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCCTGCAGAGGGGAATTTTTttggc                                                                >  SRR4302533.4341642/1‑89 (MQ=60)

                                 GAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCA                                                               <  SRR4302533.199913/94‑1 (MQ=60)

                                 GAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCA                                                               <  SRR4302533.6019725/94‑1 (MQ=60)

                                 GAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCA                                                               >  SRR4302533.1420616/1‑94 (MQ=60)

                                  AGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAA                                                              <  SRR4302533.4832842/94‑1 (MQ=60)

                                   GAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAA                                                             >  SRR4302533.2273713/1‑94 (MQ=60)

                                    AGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAT                                                            <  SRR4302533.659631/94‑1 (MQ=60)

                                    AGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAT                                                            >  SRR4302533.6178165/1‑94 (MQ=60)

                                      ATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTA                                                          >  SRR4302533.1392631/1‑94 (MQ=60)

                                      ATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTA                                                          >  SRR4302533.4174508/1‑94 (MQ=60)

                                       TACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTAC                                                         >  SRR4302533.1966342/1‑94 (MQ=60)

                                        ACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACT                                                        <  SRR4302533.1546320/94‑1 (MQ=60)

                                        ACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACT                                                        <  SRR4302533.3939176/94‑1 (MQ=60)

                                         CGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTT                                                       <  SRR4302533.6156336/94‑1 (MQ=60)

                                         CGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTT                                                       <  SRR4302533.2823093/94‑1 (MQ=60)

                                          GGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTA                                                      <  SRR4302533.3711202/94‑1 (MQ=60)

                                          GGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTA                                                      <  SRR4302533.5603255/94‑1 (MQ=60)

                                          GGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTA                                                      <  SRR4302533.1876561/94‑1 (MQ=60)

                                          GGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTA                                                      <  SRR4302533.1259338/94‑1 (MQ=60)

                                           GGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAC                                                     <  SRR4302533.4345002/94‑1 (MQ=60)

                                            GGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACC                                                    >  SRR4302533.5272245/1‑94 (MQ=60)

                                            GGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACC                                                    <  SRR4302533.2441662/94‑1 (MQ=60)

                                            GGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACC                                                    <  SRR4302533.2380017/94‑1 (MQ=60)

                                            GGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACC                                                    <  SRR4302533.4434631/94‑1 (MQ=60)

                                             GTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCG                                                   <  SRR4302533.4883543/94‑1 (MQ=60)

                                              TAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGC                                                  <  SRR4302533.879380/94‑1 (MQ=60)

                                               AGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCC                                                 <  SRR4302533.3977908/94‑1 (MQ=60)

                                                GACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTTCGGCAAATTACTTACCCCCT                                                >  SRR4302533.4422714/1‑94 (MQ=60)

                                                GACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCT                                                <  SRR4302533.841268/94‑1 (MQ=60)

                                                  CTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCCGAGGGGAATTTTTCCGGCAAATTACTTACCCCCTAA                                              >  SRR4302533.1258176/1‑94 (MQ=60)

                                                   TGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAA                                             >  SRR4302533.3371811/1‑94 (MQ=60)

                                                   TGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAA                                             <  SRR4302533.1490028/94‑1 (MQ=60)

                                                    GTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAC                                            >  SRR4302533.6056407/1‑94 (MQ=60)

                                                    GTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAAC                                            <  SRR4302533.1413461/94‑1 (MQ=60)

                                                     TTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACC                                           >  SRR4302533.3713366/1‑94 (MQ=60)

                                                      TGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCT                                          <  SRR4302533.2620791/94‑1 (MQ=60)

                                                       GCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTT                                         <  SRR4302533.4675057/94‑1 (MQ=60)

                                                        CATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTT                                        >  SRR4302533.2705872/1‑94 (MQ=60)

                                                         ATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCCTGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTT                                       >  SRR4302533.2347848/1‑94 (MQ=60)

                                                         ATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTT                                       >  SRR4302533.3350623/1‑94 (MQ=60)

                                                          TAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTT                                      <  SRR4302533.4304716/94‑1 (MQ=60)

                                                          TAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTT                                      >  SRR4302533.2794262/1‑94 (MQ=60)

                                                           AACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTT                                     <  SRR4302533.430916/94‑1 (MQ=60)

                                                           AACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTT                                     <  SRR4302533.2592361/94‑1 (MQ=60)

                                                           AACGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTT                                     <  SRR4302533.2894288/94‑1 (MQ=60)

                                                             CGTCTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCT                                   >  SRR4302533.1669697/1‑94 (MQ=60)

                                                                CTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCA                                <  SRR4302533.1180435/94‑1 (MQ=60)

                                                                CTCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCA                                <  SRR4302533.2880073/94‑1 (MQ=60)

                                                                 TCCGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAG                               <  SRR4302533.5401421/94‑1 (MQ=60)

                                                                   CGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTT                             >  SRR4302533.3023831/1‑94 (MQ=60)

                                                                   CGTGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTT                             <  SRR4302533.1606291/94‑1 (MQ=60)

                                                                     TGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCT                           <  SRR4302533.237008/94‑1 (MQ=60)

                                                                     TGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCT                           >  SRR4302533.1449340/1‑94 (MQ=60)

                                                                     TGAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCT                           >  SRR4302533.36604/1‑94 (MQ=60)

                                                                      GAATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTT                          >  SRR4302533.4917183/1‑94 (MQ=60)

                                                                       AATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTC                         >  SRR4302533.1647448/1‑94 (MQ=60)

                                                                        ATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCT                        >  SRR4302533.4923543/1‑94 (MQ=60)

                                                                        ATCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCT                        <  SRR4302533.418636/94‑1 (MQ=60)

                                                                         TCACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTT                       <  SRR4302533.5672753/94‑1 (MQ=60)

                                                                          CACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTT                      >  SRR4302533.2421850/1‑94 (MQ=60)

                                                                           ACTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTA                     >  SRR4302533.134039/1‑94 (MQ=60)

                                                                            CTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAA                    >  SRR4302533.6198154/1‑94 (MQ=60)

                                                                            CTGGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAA                    >  SRR4302533.1780800/1‑94 (MQ=60)

                                                                              GGTCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAG                  <  SRR4302533.6166696/94‑1 (MQ=60)

                                                                                TCGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTT                <  SRR4302533.1344607/94‑1 (MQ=60)

                                                                                 CGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTG               <  SRR4302533.293828/94‑1 (MQ=60)

                                                                                 CGTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTCACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTG               <  SRR4302533.4579476/94‑1 (MQ=60)

                                                                                  GTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTTCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGT              >  SRR4302533.5480251/1‑94 (MQ=60)

                                                                                  GTTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGT              <  SRR4302533.6173986/94‑1 (MQ=60)

                                                                                   TTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTC             <  SRR4302533.5930924/94‑1 (MQ=60)

                                                                                   TTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTC             >  SRR4302533.1798090/1‑94 (MQ=60)

                                                                                   TTCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTC             <  SRR4302533.5256658/94‑1 (MQ=60)

                                                                                    TCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCA            <  SRR4302533.1909121/94‑1 (MQ=60)

                                                                                    TCACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCA            <  SRR4302533.2142451/94‑1 (MQ=60)

                                                                                     CACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAC           <  SRR4302533.577252/94‑1 (MQ=60)

                                                                                      ACACCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACG          <  SRR4302533.2970130/94‑1 (MQ=60)

                                                                                          CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTG      <  SRR4302533.5893567/94‑1 (MQ=60)

                                                                                          CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTG      >  SRR4302533.294488/1‑94 (MQ=60)

                                                                                          CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTG      >  SRR4302533.4896703/1‑94 (MQ=60)

                                                                                            CCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAT    >  SRR4302533.5811863/1‑94 (MQ=60)

                                                                                             CCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATC   <  SRR4302533.4768503/94‑1 (MQ=60)

                                                                                              CTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCA  >  SRR4302533.5832397/1‑94 (MQ=60)

                                                                                              CTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCA  >  SRR4302533.530068/1‑94 (MQ=60)

ACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACA‑CCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCA  >  NC_000913/1983034‑1983220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`