Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,037,676 (C)5→6 coding (177/1005 nt) b1971 → putative reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,037,6711.C98.6% 462.6 / ‑1.5 147coding (172/1005 nt)b1971putative reductase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (82/63);  total (83/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

Only 100 of 188 total aligned reads displayed.

AAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTT  >  NC_000913/2037579‑2037758
                                                                                             |                                                                                       
aaccACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccgcccg                                                                                          <  1:284951/90‑1 (MQ=255)
  gCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccgcccg‑cc                                                                                       <  2:3519585/93‑1 (MQ=255)
    aCTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGcccg‑cccc                                                                                     <  1:89733/93‑1 (MQ=255)
     cTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGcccg‑ccccc                                                                                    <  2:4211614/93‑1 (MQ=255)
     cTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGcccg‑ccccc                                                                                    <  1:2981902/93‑1 (MQ=255)
      tGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTANCTNGTTTAAAGGGCACGATCGCCCGcccg‑cccccc                                                                                   >  2:4833413/1‑92 (MQ=255)
       gggCATCAGTGGAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGATTAAAGGGAACGATCGCCCGcccg‑cgccctg                                                                                  >  2:3047275/1‑91 (MQ=255)
        ggCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGCGAACGATCGCCCGcccg‑ccccccgc                                                                                 >  1:266969/1‑90 (MQ=255)
           aTCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCgg                                                                               >  1:1899555/1‑93 (MQ=255)
           aTCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCgg                                                                               >  2:5754906/1‑93 (MQ=255)
            tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGa                                                                              >  2:4318784/1‑93 (MQ=255)
            tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGa                                                                              >  1:3616566/1‑93 (MQ=255)
              aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGaaa                                                                            >  1:4538264/1‑93 (MQ=255)
               gTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGaaaa                                                                           <  1:1447476/93‑1 (MQ=255)
                tGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAg                                                                          <  1:2248629/93‑1 (MQ=255)
                  cAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAgcg                                                                        <  1:3244513/93‑1 (MQ=255)
                   aaCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAgcgc                                                                       <  1:1847083/93‑1 (MQ=255)
                   aaCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAgcgc                                                                       <  1:1681161/93‑1 (MQ=255)
                   aaCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAgcgc                                                                       >  1:5657150/1‑93 (MQ=255)
                    aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCt                                                                      >  1:430532/1‑93 (MQ=255)
                     cTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTg                                                                     >  2:3694277/1‑93 (MQ=255)
                     cTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCCCTg                                                                     >  2:3923318/1‑93 (MQ=255)
                      tGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTgg                                                                    >  2:2416693/1‑93 (MQ=255)
                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGa                                                                   <  2:5032928/93‑1 (MQ=255)
                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGa                                                                   <  2:1391069/93‑1 (MQ=255)
                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGa                                                                   >  1:5737023/1‑93 (MQ=255)
                         aCTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGt                                                                 >  1:4979843/1‑93 (MQ=255)
                          ctttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCTCCCGCCCCCCGCCGGAAAAGCGCTGGAGtt                                                                >  1:1352493/1‑93 (MQ=255)
                          ctttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGtt                                                                >  2:2456845/1‑93 (MQ=255)
                           tttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTc                                                               <  1:2045513/93‑1 (MQ=255)
                             tctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAg                                                             <  1:5703381/93‑1 (MQ=255)
                              ctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGc                                                            <  1:2896301/93‑1 (MQ=255)
                               tttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCa                                                           <  2:6294915/93‑1 (MQ=255)
                                  gCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGc                                                        <  2:5662547/93‑1 (MQ=255)
                                  gCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGc                                                        >  2:3286016/1‑93 (MQ=255)
                                   ccTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGcc                                                       <  1:1993634/93‑1 (MQ=255)
                                    cTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCt                                                      >  1:4623238/1‑93 (MQ=255)
                                     tCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTg                                                     >  1:2446548/1‑93 (MQ=255)
                                     tCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTg                                                     >  1:3300147/1‑93 (MQ=255)
                                       aCGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTgcc                                                   >  1:2110267/1‑93 (MQ=255)
                                         gCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTgccgc                                                 <  1:5622389/93‑1 (MQ=255)
                                          cTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTgccgcc                                                >  2:725923/1‑93 (MQ=255)
                                            gcgcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTg                                              >  1:1217195/1‑93 (MQ=255)
                                            gcgcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTg                                              <  2:3940897/93‑1 (MQ=255)
                                            gcgcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTg                                              <  2:1365065/93‑1 (MQ=255)
                                              gcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGc                                            >  1:4594717/1‑93 (MQ=255)
                                              gcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGc                                            >  2:4581826/1‑93 (MQ=255)
                                                aTGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaa                                          >  1:1214376/1‑93 (MQ=255)
                                                 tGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaaa                                         >  1:3686851/1‑93 (MQ=255)
                                                 tGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaaa                                         >  1:6166792/1‑93 (MQ=255)
                                                  gCCTATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaaaa                                        <  1:917429/93‑1 (MQ=255)
                                                   ccGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAAt                                       <  2:4436187/93‑1 (MQ=255)
                                                    cGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATa                                      <  1:4853718/93‑1 (MQ=255)
                                                    cGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATa                                      >  2:472974/1‑93 (MQ=255)
                                                      aTCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAc                                    >  2:2109227/1‑93 (MQ=255)
                                                      aTCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAc                                    >  2:1736321/1‑93 (MQ=255)
                                                       tCTGCTTAGCTGTTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCCCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                   <  2:5371939/93‑1 (MQ=255)
                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAct                                   >  2:4162909/1‑92 (MQ=255)
                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                   >  2:2600602/1‑93 (MQ=255)
                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                   <  1:5854659/93‑1 (MQ=255)
                                                        cTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCt                                  <  2:922536/93‑1 (MQ=255)
                                                         tGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTg                                 >  2:5822275/1‑93 (MQ=255)
                                                         tGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTg                                 >  2:520410/1‑93 (MQ=255)
                                                         tGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTg                                 <  2:3815563/93‑1 (MQ=255)
                                                          gCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGc                                >  2:5746427/1‑93 (MQ=255)
                                                           cTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGcc                               <  1:2589160/93‑1 (MQ=255)
                                                           cTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGcc                               >  2:4793549/1‑93 (MQ=255)
                                                            ttAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCa                              <  2:2789802/93‑1 (MQ=255)
                                                            ttAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCa                              <  2:6135318/93‑1 (MQ=255)
                                                             tAGCTGGTTTAAAGGAAACGACCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCAc                             <  1:726731/93‑1 (MQ=255)
                                                               gCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCATCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTg                           >  1:6282290/1‑93 (MQ=255)
                                                                cgggTTTAAGGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGCAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGa                          <  1:3371871/91‑1 (MQ=255)
                                                                  ggTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACg                        <  1:6038827/93‑1 (MQ=255)
                                                                   gTTTAAAGGAAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGc                       <  1:3810316/93‑1 (MQ=255)
                                                                    tttAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGcc                      >  2:845896/1‑93 (MQ=255)
                                                                    tttAAAGGGAACGACCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGcc                      <  2:4616822/93‑1 (MQ=255)
                                                                     ttAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCca                     >  1:5446746/1‑93 (MQ=255)
                                                                     ttAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCca                     <  2:2683058/93‑1 (MQ=255)
                                                                        aaGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCcagca                  <  1:3556588/93‑1 (MQ=255)
                                                                         aGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCcagcag                 >  1:5346347/1‑93 (MQ=255)
                                                                          gggAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGa                >  2:3168374/1‑93 (MQ=255)
                                                                           ggACCGAGCCCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGAt               <  1:2552795/93‑1 (MQ=255)
                                                                           ggAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGAt               >  1:2565315/1‑93 (MQ=255)
                                                                              aCGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATaaa            <  1:4160091/93‑1 (MQ=255)
                                                                              aCGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATaaa            <  2:5974695/93‑1 (MQ=255)
                                                                                gaacgccctcccgccCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAAAGt          <  1:5172664/90‑1 (MQ=255)
                                                                                 aTCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCGGCAGATAAAGtt         >  1:5595312/1‑93 (MQ=255)
                                                                                 aTCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGtt         >  1:2394668/1‑93 (MQ=255)
                                                                                   cccccccccc‑ccccCGCCGGAAAGGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc      <  1:5835197/91‑1 (MQ=255)
                                                                                    gcccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc      <  1:5228014/93‑1 (MQ=255)
                                                                                    gcccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc      >  1:6070648/1‑93 (MQ=255)
                                                                                    ccccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc      <  1:1904420/92‑1 (MQ=255)
                                                                                     cccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCTCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg     >  2:1796871/1‑93 (MQ=255)
                                                                                     cccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAAAGTTTCCg     <  1:1475135/93‑1 (MQ=255)
                                                                                     cccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg     >  1:1234128/1‑93 (MQ=255)
                                                                                     cccgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg     >  2:104988/1‑93 (MQ=255)
                                                                                       cgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGt   >  2:5686469/1‑93 (MQ=255)
                                                                                       cgcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGt   >  1:98413/1‑93 (MQ=255)
                                                                                        gcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGtt  >  1:4596704/1‑93 (MQ=255)
                                                                                        gcccgcccCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGtt  >  2:2045413/1‑93 (MQ=255)
                                                                                             |                                                                                       
AAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTT  >  NC_000913/2037579‑2037758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTC  >  NC_000913/2037583‑2037769
                                                                                              |                                                                                             
ACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCGCG‑CCCCC                                                                                               >  SRR4302533.1231307/1‑94 (MQ=60)
ACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCC                                                                                               <  SRR4302533.90301/94‑1 (MQ=60)
 CTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCC                                                                                              <  SRR4302533.3000142/94‑1 (MQ=60)
  TGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCcg                                                                                             <  SRR4302533.2046939/94‑3 (MQ=60)
   GGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGCGAACGATCGCCCGCCCG‑CCCCCcgc                                                                                            >  SRR4302533.268592/1‑91 (MQ=60)
    GGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCC                                                                                            <  SRR4302533.3732289/94‑1 (MQ=60)
      CATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGG                                                                                          >  SRR4302533.1911152/1‑94 (MQ=60)
       ATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGA                                                                                         >  SRR4302533.3638814/1‑94 (MQ=60)
         CAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAA                                                                                       >  SRR4302533.4565741/1‑94 (MQ=60)
           GTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAG                                                                                     <  SRR4302533.1369018/94‑1 (MQ=60)
           GTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAG                                                                                     <  SRR4302533.1456209/94‑1 (MQ=60)
            TGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGC                                                                                    >  SRR4302533.3264106/1‑94 (MQ=60)
            TGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGC                                                                                    <  SRR4302533.2262524/94‑1 (MQ=60)
            TGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGC                                                                                    >  SRR4302533.4919440/1‑94 (MQ=60)
             GCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCG                                                                                   <  SRR4302533.3606447/94‑1 (MQ=60)
              CAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGC                                                                                  <  SRR4302533.3264512/94‑1 (MQ=60)
              CAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGC                                                                                  >  SRR4302533.5691335/1‑94 (MQ=60)
               AACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCT                                                                                 >  SRR4302533.433099/1‑94 (MQ=60)
               AACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCT                                                                                 <  SRR4302533.1858343/94‑1 (MQ=60)
               AACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCT                                                                                 <  SRR4302533.1691344/94‑1 (MQ=60)
                  TGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGA                                                                              >  SRR4302533.5771651/1‑94 (MQ=60)
                    CACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGT                                                                            >  SRR4302533.5010038/1‑94 (MQ=60)
                     ACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCTCCCGCCCCCCGCCGGAAAAGCGCTGGAGTT                                                                           >  SRR4302533.1360627/1‑94 (MQ=60)
                       TTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCA                                                                         <  SRR4302533.2058123/94‑1 (MQ=60)
                       TTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCA                                                                         >  SRR4302533.5373515/1‑94 (MQ=60)
                         TCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGC                                                                       <  SRR4302533.5737830/94‑1 (MQ=60)
                          CTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCA                                                                      <  SRR4302533.2914109/94‑1 (MQ=60)
                               CCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCT                                                                 <  SRR4302533.2005863/94‑1 (MQ=60)
                               CCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCT                                                                 <  SRR4302533.4339838/94‑1 (MQ=60)
                               CCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCT                                                                 >  SRR4302533.4651262/1‑94 (MQ=60)
                                CTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTG                                                                >  SRR4302533.2461647/1‑94 (MQ=60)
                                CTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTG                                                                <  SRR4302533.1800660/94‑1 (MQ=60)
                                CTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTG                                                                >  SRR4302533.3320519/1‑94 (MQ=60)
                                 TCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCTGAAAAGCGCTGGAGTTCAGCAAGCCTGC                                                               >  SRR4302533.6098051/1‑94 (MQ=60)
                                  CACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCC                                                              >  SRR4302533.2123282/1‑94 (MQ=60)
                                  CACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCC                                                              <  SRR4302533.6039017/94‑1 (MQ=60)
                                   ccgcgccgtATGCCGACCTGCTTGGTTGGTTGAAAGGAAGCGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCG                                                             <  SRR4302533.2622135/85‑1 (MQ=53)
                                     GCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCC                                                           <  SRR4302533.5656372/94‑1 (MQ=60)
                                     GCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCC                                                           >  SRR4302533.1631772/1‑94 (MQ=60)
                                       TGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTG                                                         >  SRR4302533.1224504/1‑94 (MQ=60)
                                         CGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGC                                                       >  SRR4302533.4622551/1‑94 (MQ=60)
                                         CGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGC                                                       >  SRR4302533.5746634/1‑94 (MQ=60)
                                           CATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAA                                                     >  SRR4302533.1221672/1‑94 (MQ=60)
                                           CATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAA                                                     <  SRR4302533.5247954/94‑1 (MQ=60)
                                            ATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAA                                                    <  SRR4302533.4028010/94‑1 (MQ=60)
                                            ATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAA                                                    >  SRR4302533.3709511/1‑94 (MQ=60)
                                            ATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAA                                                    >  SRR4302533.6203854/1‑94 (MQ=60)
                                              GCCTATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAAT                                                  <  SRR4302533.922819/94‑1 (MQ=60)
                                               CCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATA                                                 >  SRR4302533.2308972/1‑94 (MQ=60)
                                               CCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAA                                                >  SRR4302533.4614042/1‑94 (MQ=60)
                                                CGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAA                                                >  SRR4302533.1962624/1‑94 (MQ=60)
                                                CGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAA                                                >  SRR4302533.5685382/1‑94 (MQ=60)
                                                CGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAA                                                <  SRR4302533.4883130/94‑1 (MQ=60)
                                                CGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAA                                                >  SRR4302533.2633775/1‑94 (MQ=60)
                                                 GATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAC                                               <  SRR4302533.694762/94‑1 (MQ=60)
                                                   TCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCT                                             <  SRR4302533.5890056/94‑1 (MQ=60)
                                                    CTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTG                                            <  SRR4302533.5878101/94‑1 (MQ=60)
                                                     TGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGC                                           >  SRR4302533.2603250/1‑94 (MQ=60)
                                                      GCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCC                                          <  SRR4302533.2129534/94‑1 (MQ=60)
                                                       CTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCA                                         <  SRR4302533.2605073/94‑1 (MQ=60)
                                                       CTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCA                                         >  SRR4302533.2122016/1‑94 (MQ=60)
                                                         TAGCTGGTTTAAAGGAAACGACCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACT                                       <  SRR4302533.731024/94‑1 (MQ=60)
                                                          AGCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCATCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTG                                      >  SRR4302533.6319784/1‑94 (MQ=60)
                                                           GCTGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCGGCCGCCTGGCAACATAAGCTGCCACTGA                                     >  SRR4302533.2429135/1‑94 (MQ=60)
                                                            CGGGTTTAAGGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGCAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGAC                                    <  SRR4302533.3392692/94‑1 (MQ=60)
                                                             TGGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACG                                   <  SRR4302533.190825/94‑1 (MQ=60)
                                                             TGGTTAAAAGGGAAGGAGCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACG                                   <  SRR4302533.3285674/94‑1 (MQ=60)
                                                              GGTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGC                                  <  SRR4302533.6075423/94‑1 (MQ=60)
                                                               GTTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCC                                 <  SRR4302533.1482270/94‑1 (MQ=60)
                                                               GTTTAAAGGAAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCC                                 <  SRR4302533.3833621/94‑1 (MQ=60)
                                                                TTTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCA                                >  SRR4302533.5479558/1‑94 (MQ=60)
                                                                 TTAAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAG                               <  SRR4302533.5705332/94‑1 (MQ=60)
                                                                   AAAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCA                             >  SRR4302533.4325953/1‑94 (MQ=60)
                                                                    AAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAG                            >  SRR4302533.2467839/1‑94 (MQ=60)
                                                                    AAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAG                            <  SRR4302533.4695920/94‑1 (MQ=60)
                                                                    AAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAG                            >  SRR4302533.5378417/1‑94 (MQ=60)
                                                                    AAGGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAG                            <  SRR4302533.3578507/94‑1 (MQ=60)
                                                                      GGGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGAT                          >  SRR4302533.2581102/1‑94 (MQ=60)
                                                                       ggaccgagccCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATA                         <  SRR4302533.2568504/84‑1 (MQ=60)
                                                                       GGAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATA                         >  SRR4302533.1331865/1‑94 (MQ=60)
                                                                        GAACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAA                        <  SRR4302533.4637971/94‑1 (MQ=60)
                                                                        GAACGACCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAA                        <  SRR4302533.5799798/94‑1 (MQ=60)
                                                                          ACGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAG                      <  SRR4302533.4185304/94‑1 (MQ=60)
                                                                           CGATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGT                     >  SRR4302533.920128/1‑94 (MQ=60)
                                                                            GATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCGGCAGATAAAGTT                    >  SRR4302533.5629120/1‑94 (MQ=60)
                                                                            GATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTT                    >  SRR4302533.2862795/1‑94 (MQ=60)
                                                                            GATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTT                    >  SRR4302533.2409449/1‑94 (MQ=60)
                                                                            GATCGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTT                    >  SRR4302533.2934758/1‑94 (MQ=60)
                                                                            GAACGCCCTCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAAAGTT                    <  SRR4302533.5203874/94‑1 (MQ=60)
                                                                               CGCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCC                 >  SRR4302533.6107379/1‑94 (MQ=60)
                                                                                ccccccccccCCCCCGCCGGAAAGGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                <  SRR4302533.5870459/84‑1 (MQ=60)
                                                                                GCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                >  SRR4302533.1241508/1‑94 (MQ=60)
                                                                                GCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                >  SRR4302533.2902362/1‑94 (MQ=60)
                                                                                GCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                >  SRR4302533.5637203/1‑94 (MQ=60)
                                                                                GCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                <  SRR4302533.5259467/94‑1 (MQ=60)
                                                                                GCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                >  SRR4302533.5035552/1‑94 (MQ=60)
                                                                                CCCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCG                <  SRR4302533.1916041/94‑1 (MQ=60)
                                                                                 CCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATACCCTGCCACTGACGCCAGCAGATAAAGTTTCCGG               <  SRR4302533.1484051/94‑1 (MQ=60)
                                                                                 CCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGG               >  SRR4302533.755050/1‑94 (MQ=60)
                                                                                 CCCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGG               <  SRR4302533.3694459/94‑1 (MQ=60)
                                                                                  CCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGT              >  SRR4302533.99023/1‑94 (MQ=60)
                                                                                  CCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGT              >  SRR4302533.961707/1‑94 (MQ=60)
                                                                                  CCGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGT              >  SRR4302533.620743/1‑94 (MQ=60)
                                                                                   CGCCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTT             >  SRR4302533.4624550/1‑94 (MQ=60)
                                                                                    ccccgcCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTA            <  SRR4302533.1442341/88‑1 (MQ=60)
                                                                                     CCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATATCCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTAT           <  SRR4302533.786103/94‑1 (MQ=60)
                                                                                     CCCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTAT           >  SRR4302533.3576154/1‑94 (MQ=60)
                                                                                     CCCGCCCCCCGCCGGAAAAGCGATGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTAT           <  SRR4302533.2888591/94‑1 (MQ=60)
                                                                                      CCGCCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATA          >  SRR4302533.3294182/1‑94 (MQ=60)
                                                                                       cgcCCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAA         <  SRR4302533.2150993/91‑1 (MQ=60)
                                                                                        C‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATA       >  SRR4302533.2667133/1‑94 (MQ=60)
                                                                                          CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAA      >  SRR4302533.5265361/1‑94 (MQ=60)
                                                                                          CCCCCGCCGGAAAAGCGCTAGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAA      >  SRR4302533.5956749/1‑94 (MQ=60)
                                                                                            CCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT    >  SRR4302533.3928371/1‑94 (MQ=60)
                                                                                            CCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACT    >  SRR4302533.924649/1‑94 (MQ=60)
                                                                                             CCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTT   >  SRR4302533.4291067/1‑94 (MQ=60)
                                                                                             CCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTT   >  SRR4302533.2555916/1‑94 (MQ=60)
                                                                                              CGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAACATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTC  >  SRR4302533.4092933/1‑94 (MQ=60)
                                                                                              CGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTC  >  SRR4302533.2558479/1‑94 (MQ=60)
                                                                                              |                                                                                             
ACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCGCCCG‑CCCCCGCCGGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATAATAACTTC  >  NC_000913/2037583‑2037769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑