Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,038,965 T→C V154A (GTC→GCC)  b1972 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,038,9650TC100.0% 637.9 / NA 196V154A (GTC→GCC) b1972hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (95/101);  total (95/101)

Only 100 of 200 total aligned reads displayed.

GCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTG  >  NC_000913/2038875‑2039054
                                                                                          |                                                                                         
gCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCt                                                                                         >  2:2820283/1‑93 (MQ=255)
gCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCt                                                                                         >  1:4005531/1‑93 (MQ=255)
 cTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTa                                                                                        >  1:138505/1‑93 (MQ=255)
 cTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTa                                                                                        <  2:928170/93‑1 (MQ=255)
  tGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTAt                                                                                       >  1:590433/1‑93 (MQ=255)
   gggTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATc                                                                                      >  2:1336362/1‑93 (MQ=255)
    ggTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCt                                                                                     <  2:1084896/93‑1 (MQ=255)
    ggTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCt                                                                                     >  2:2653192/1‑93 (MQ=255)
     gTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCtt                                                                                    <  2:4005401/93‑1 (MQ=255)
      tAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTg                                                                                   <  2:4682163/93‑1 (MQ=255)
       aaTTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGt                                                                                  >  2:1970242/1‑93 (MQ=255)
        aTTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTc                                                                                 >  1:5212589/1‑93 (MQ=255)
         ttttGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTcg                                                                                >  2:3525224/1‑93 (MQ=255)
          tttGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTcgc                                                                               <  1:2424843/93‑1 (MQ=255)
               ttgcttTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTc                                                                          >  2:1612268/1‑93 (MQ=255)
               ttgcttTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTc                                                                          <  1:5817856/93‑1 (MQ=255)
               ttgcttTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTc                                                                          <  1:5600942/93‑1 (MQ=255)
                tgcttTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCt                                                                         <  1:5612106/93‑1 (MQ=255)
                 gcttTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTg                                                                        >  1:3866958/1‑93 (MQ=255)
                   tttAGCGTTCACTTCAACCCAGGCGATGCAGCGAAACCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCAATCTTGTCGCTATTCTGGc                                                                      <  2:6111065/93‑1 (MQ=255)
                   tttAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGc                                                                      <  2:4140876/93‑1 (MQ=255)
                     tAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGccc                                                                    >  1:3551401/1‑93 (MQ=255)
                      aGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGcccc                                                                   >  2:5793609/1‑93 (MQ=255)
                      aGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGcccc                                                                   <  1:4604520/93‑1 (MQ=255)
                         gTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAAt                                                                <  1:3479863/93‑1 (MQ=255)
                         gTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAAt                                                                >  2:1721400/1‑93 (MQ=255)
                         gTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAAt                                                                <  2:3694837/93‑1 (MQ=255)
                          ttCACTGCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATa                                                               <  2:2340361/93‑1 (MQ=255)
                           tCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATAc                                                              >  1:848496/1‑93 (MQ=255)
                            cACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACa                                                             >  2:4628804/1‑93 (MQ=255)
                              cTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACAtt                                                           <  1:1029923/93‑1 (MQ=255)
                              cTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACAtt                                                           <  1:3702155/93‑1 (MQ=255)
                               ttCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTa                                                          >  2:4469790/1‑93 (MQ=255)
                               ttCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTa                                                          <  2:3892858/93‑1 (MQ=255)
                                 cAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATc                                                        <  2:4432967/93‑1 (MQ=255)
                                   aCCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCtg                                                      >  2:2132896/1‑93 (MQ=255)
                                   aCCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCtg                                                      <  2:1912641/93‑1 (MQ=255)
                                    cccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCtgt                                                     <  1:4241663/93‑1 (MQ=255)
                                     ccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCtgtg                                                    >  2:6265941/1‑93 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCGAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   <  2:4066682/93‑1 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   >  1:4383284/1‑93 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   >  2:701086/1‑93 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   <  1:1889223/93‑1 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   <  1:731152/93‑1 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   <  1:6206852/93‑1 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   >  1:2486269/1‑93 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   >  2:5401918/1‑93 (MQ=255)
                                      cAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTgg                                                   >  2:3569982/1‑93 (MQ=255)
                                       aGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGt                                                  >  1:2886806/1‑93 (MQ=255)
                                       aGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTAGCCCCAATACATTATCTGTGGt                                                  >  1:5742297/1‑93 (MQ=255)
                                          cGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCg                                               >  1:1828535/1‑93 (MQ=255)
                                           gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGt                                              >  2:1476000/1‑93 (MQ=255)
                                           gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGt                                              <  2:5027405/93‑1 (MQ=255)
                                            aTGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTg                                             >  2:1112899/1‑93 (MQ=255)
                                              gCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa                                           <  1:2571117/93‑1 (MQ=255)
                                              gCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa                                           <  2:2550001/93‑1 (MQ=255)
                                                aGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                         <  1:4375149/93‑1 (MQ=255)
                                                aGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                         <  1:2949538/93‑1 (MQ=255)
                                                aGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAAAACATTATCTGTGGTCCGTGAAGa                                         <  2:5119353/93‑1 (MQ=255)
                                                  cGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGatt                                       <  2:22223/93‑1 (MQ=255)
                                                   gAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGatta                                      >  1:3627459/1‑93 (MQ=255)
                                                   gAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGatta                                      <  1:4914253/93‑1 (MQ=255)
                                                   gAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGatta                                      >  1:3458068/1‑93 (MQ=255)
                                                    aaaaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGattat                                     <  2:3108885/93‑1 (MQ=255)
                                                    aaaaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGattat                                     <  2:4300026/93‑1 (MQ=255)
                                                    aaaaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGattat                                     >  1:1864442/1‑93 (MQ=255)
                                                     aaaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGattatt                                    >  1:3747433/1‑93 (MQ=255)
                                                     aaaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGattatt                                    <  1:1429839/93‑1 (MQ=255)
                                                      aaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAttt                                   >  2:5060591/1‑93 (MQ=255)
                                                      aaCTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAttt                                   <  1:1534261/93‑1 (MQ=255)
                                                        cTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa                                 <  1:781199/93‑1 (MQ=255)
                                                         tCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAc                                <  1:2525535/93‑1 (MQ=255)
                                                         tCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAc                                <  1:5635351/93‑1 (MQ=255)
                                                         tCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAc                                >  1:3372734/1‑93 (MQ=255)
                                                             cAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCa                            <  1:815639/93‑1 (MQ=255)
                                                             cAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCa                            <  1:6081167/93‑1 (MQ=255)
                                                              aaGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAg                           >  2:2877844/1‑93 (MQ=255)
                                                              aaGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAg                           <  1:3570554/93‑1 (MQ=255)
                                                               aGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGc                          <  1:231981/93‑1 (MQ=255)
                                                                  aCTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGc                       >  2:1213067/1‑93 (MQ=255)
                                                                  aCTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGc                       <  2:3632992/93‑1 (MQ=255)
                                                                  aCTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGc                       >  1:3339357/1‑93 (MQ=255)
                                                                   cTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCt                      >  2:1560188/1‑93 (MQ=255)
                                                                     ggCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCa                    <  1:3215087/93‑1 (MQ=255)
                                                                     ggCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCa                    >  2:5856273/1‑93 (MQ=255)
                                                                     ggCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCa                    <  2:2496940/93‑1 (MQ=255)
                                                                      gCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAt                   <  2:3048786/93‑1 (MQ=255)
                                                                       cAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATc                  >  2:3464870/1‑93 (MQ=255)
                                                                         aCAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTa                <  2:3238222/93‑1 (MQ=255)
                                                                         aCAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTa                <  1:987383/93‑1 (MQ=255)
                                                                            gTTGCGTAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgc             >  2:568301/1‑93 (MQ=255)
                                                                            gTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgc             >  1:4557154/1‑93 (MQ=255)
                                                                            gTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgc             >  1:3774851/1‑93 (MQ=255)
                                                                             ttGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgct            <  1:5422556/93‑1 (MQ=255)
                                                                               gCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgctgg          <  2:2514384/93‑1 (MQ=255)
                                                                               gCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgctgg          >  2:5602648/1‑93 (MQ=255)
                                                                                cATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgctggg         <  1:3137205/93‑1 (MQ=255)
                                                                                   aaTTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgctgggctg      >  1:1467544/1‑93 (MQ=255)
                                                                                   aaTTTCGCATATCTTGTCGTGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACgctgggctg      >  2:6165897/1‑93 (MQ=255)
                                                                                     tttCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGggctggc    <  1:4046848/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTG  >  NC_000913/2038875‑2039054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGTA  >  NC_000913/2038872‑2039056
                                                                                             |                                                                                           
TCAGCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGC                                                                                             >  SRR4302533.3093104/1‑94 (MQ=60)
 CAGCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCC                                                                                            >  SRR4302533.5293755/1‑94 (MQ=60)
  AGCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCT                                                                                           >  SRR4302533.4029914/1‑94 (MQ=60)
   GCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTA                                                                                          >  SRR4302533.139386/1‑94 (MQ=60)
    CTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTAT                                                                                         >  SRR4302533.593918/1‑94 (MQ=60)
    CTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTAT                                                                                         >  SRR4302533.5636977/1‑94 (MQ=60)
     TGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATC                                                                                        <  SRR4302533.326274/94‑1 (MQ=60)
          AATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTC                                                                                   >  SRR4302533.5243979/1‑94 (MQ=60)
             TTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCG                                                                                <  SRR4302533.2439813/94‑1 (MQ=60)
               TGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGAT                                                                              >  SRR4302533.1156502/1‑94 (MQ=60)
                 CTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTC                                                                            >  SRR4302533.9937/1‑94 (MQ=60)
                  TTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCT                                                                           <  SRR4302533.3496301/94‑1 (MQ=60)
                  TTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCT                                                                           <  SRR4302533.5853000/94‑1 (MQ=60)
                  TTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCT                                                                           <  SRR4302533.5634785/94‑1 (MQ=60)
                   TGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTG                                                                          <  SRR4302533.5646027/94‑1 (MQ=60)
                   TGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTG                                                                          >  SRR4302533.3890561/1‑94 (MQ=60)
                     CTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGC                                                                        >  SRR4302533.3579925/1‑94 (MQ=60)
                       TTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCC                                                                      >  SRR4302533.3573279/1‑94 (MQ=60)
                         AGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCA                                                                    <  SRR4302533.4632415/94‑1 (MQ=60)
                          GCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAA                                                                   >  SRR4302533.3364847/1‑94 (MQ=60)
                           CGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAAT                                                                  <  SRR4302533.2487249/94‑1 (MQ=60)
                            GTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATA                                                                 <  SRR4302533.3501333/94‑1 (MQ=60)
                             TTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATAC                                                                <  SRR4302533.2961068/94‑1 (MQ=60)
                             TTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATAC                                                                >  SRR4302533.853464/1‑94 (MQ=60)
                                ACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATT                                                             >  SRR4302533.25405/1‑94 (MQ=60)
                                ACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATT                                                             >  SRR4302533.4587226/1‑94 (MQ=60)
                                 CTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTA                                                            <  SRR4302533.3724886/94‑1 (MQ=60)
                                 CTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTA                                                            <  SRR4302533.1035977/94‑1 (MQ=60)
                                  TTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTAT                                                           >  SRR4302533.4149153/1‑94 (MQ=60)
                                    CAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCT                                                         >  SRR4302533.1872681/1‑94 (MQ=60)
                                     AACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTG                                                        <  SRR4302533.5509821/94‑1 (MQ=60)
                                     AACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTAGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTG                                                        >  SRR4302533.5664985/1‑94 (MQ=60)
                                       CCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTG                                                      <  SRR4302533.4267278/94‑1 (MQ=60)
                                        CCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGG                                                     >  SRR4302533.4794642/1‑94 (MQ=60)
                                        CCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGG                                                     >  SRR4302533.4409746/1‑94 (MQ=60)
                                        CCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGG                                                     >  SRR4302533.4289329/1‑94 (MQ=60)
                                        CCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGG                                                     >  SRR4302533.2501588/1‑94 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGT                                                    <  SRR4302533.735458/94‑1 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGT                                                    <  SRR4302533.6244073/94‑1 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGT                                                    <  SRR4302533.1900761/94‑1 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGT                                                    >  SRR4302533.2904557/1‑94 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGT                                                    >  SRR4302533.3753826/1‑94 (MQ=60)
                                         CAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTAGCCCCAATACATTATCTGTGGT                                                    >  SRR4302533.5776947/1‑94 (MQ=60)
                                          AGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTC                                                   >  SRR4302533.3181668/1‑94 (MQ=60)
                                           GGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCC                                                  <  SRR4302533.223268/94‑1 (MQ=60)
                                            GCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCG                                                 >  SRR4302533.1839701/1‑94 (MQ=60)
                                               ATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGA                                              <  SRR4302533.5838044/94‑1 (MQ=60)
                                                 GCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAG                                            <  SRR4302533.2586939/94‑1 (MQ=60)
                                                 GCAGCGAAAACTCGGCAAGCACNGGNAACAGTTGCATNATNTCGCNTATCTNGTNGCGATTCTNGCNCCANNNCNTTATCNGNGGNCNNNGNAG                                            >  SRR4302533.1799509/1‑94 (MQ=55)
                                                  CAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGA                                           <  SRR4302533.6158394/94‑1 (MQ=60)
                                                   AGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAT                                          <  SRR4302533.5334090/94‑1 (MQ=60)
                                                   AGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAT                                          <  SRR4302533.4401556/94‑1 (MQ=60)
                                                   AGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAT                                          >  SRR4302533.3118525/1‑94 (MQ=60)
                                                   AGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAT                                          <  SRR4302533.2967577/94‑1 (MQ=60)
                                                     CGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTA                                        >  SRR4302533.7498/1‑94 (MQ=60)
                                                     CGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTA                                        <  SRR4302533.1558014/94‑1 (MQ=60)
                                                     CGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTA                                        >  SRR4302533.3479415/1‑94 (MQ=60)
                                                     CGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTA                                        >  SRR4302533.3649779/1‑94 (MQ=60)
                                                      GAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAT                                       <  SRR4302533.4576791/94‑1 (MQ=60)
                                                      GAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAT                                       <  SRR4302533.4944021/94‑1 (MQ=60)
                                                      GAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAT                                       >  SRR4302533.1875817/1‑94 (MQ=60)
                                                       AAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATT                                      >  SRR4302533.3770409/1‑94 (MQ=60)
                                                        AAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTT                                     <  SRR4302533.1438481/94‑1 (MQ=60)
                                                        AAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTT                                     <  SRR4302533.1463279/94‑1 (MQ=60)
                                                         AACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTC                                    <  SRR4302533.1543478/94‑1 (MQ=60)
                                                          ACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCA                                   <  SRR4302533.2784947/94‑1 (MQ=60)
                                                           CTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAC                                  >  SRR4302533.4406941/1‑94 (MQ=60)
                                                           CTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAC                                  <  SRR4302533.785800/94‑1 (MQ=60)
                                                           CTCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCAC                                  >  SRR4302533.3393561/1‑94 (MQ=60)
                                                            TCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACC                                 <  SRR4302533.5669430/94‑1 (MQ=60)
                                                            TCGGCAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACC                                 <  SRR4302533.2541086/94‑1 (MQ=60)
                                                                CAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAG                             <  SRR4302533.5936652/94‑1 (MQ=60)
                                                                CAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAG                             <  SRR4302533.6117941/94‑1 (MQ=60)
                                                                CAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAG                             <  SRR4302533.820421/94‑1 (MQ=60)
                                                                CAAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAG                             >  SRR4302533.2582536/1‑94 (MQ=60)
                                                                 AAGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGC                            <  SRR4302533.3592558/94‑1 (MQ=60)
                                                                  AGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCC                           <  SRR4302533.1887684/94‑1 (MQ=60)
                                                                  AGCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCC                           <  SRR4302533.233414/94‑1 (MQ=60)
                                                                   GCACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTATGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCG                          >  SRR4302533.3406237/1‑94 (MQ=60)
                                                                    CACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGC                         >  SRR4302533.3638231/1‑94 (MQ=60)
                                                                    CACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGC                         >  SRR4302533.3359997/1‑94 (MQ=60)
                                                                    CACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGC                         >  SRR4302533.830716/1‑94 (MQ=60)
                                                                     ACTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCT                        >  SRR4302533.4145240/1‑94 (MQ=60)
                                                                      CTGGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTC                       <  SRR4302533.796029/94‑1 (MQ=60)
                                                                        GGCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCAT                     <  SRR4302533.3234909/94‑1 (MQ=60)
                                                                         GCAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATC                    >  SRR4302533.385341/1‑94 (MQ=60)
                                                                          CAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCT                   <  SRR4302533.2434126/94‑1 (MQ=60)
                                                                          CAACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCT                   >  SRR4302533.1489568/1‑94 (MQ=60)
                                                                           AACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTA                  >  SRR4302533.6013281/1‑94 (MQ=60)
                                                                            ACAGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTAC                 <  SRR4302533.993162/94‑1 (MQ=60)
                                                                              AGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGC               >  SRR4302533.3545734/1‑94 (MQ=60)
                                                                              AGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGC               >  SRR4302533.3789552/1‑94 (MQ=60)
                                                                              AGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGC               >  SRR4302533.3797990/1‑94 (MQ=60)
                                                                              AGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGC               >  SRR4302533.1428400/1‑94 (MQ=60)
                                                                              AGTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGC               >  SRR4302533.4584791/1‑94 (MQ=60)
                                                                               GTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCT              >  SRR4302533.3930101/1‑94 (MQ=60)
                                                                               GTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCT              <  SRR4302533.5734149/94‑1 (MQ=60)
                                                                               GTTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCT              >  SRR4302533.3716347/1‑94 (MQ=60)
                                                                                TTGCATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTG             <  SRR4302533.5455186/94‑1 (MQ=60)
                                                                                   CATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGC          >  SRR4302533.6266334/1‑94 (MQ=60)
                                                                                   CATAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGC          <  SRR4302533.3156494/94‑1 (MQ=60)
                                                                                     TAATTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTG        >  SRR4302533.1476394/1‑94 (MQ=60)
                                                                                        TTTCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCT     <  SRR4302533.4071487/94‑1 (MQ=60)
                                                                                          TCGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGT   <  SRR4302533.5088679/94‑1 (MQ=60)
                                                                                           CGCCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGTA  >  SRR4302533.2467490/1‑94 (MQ=60)
                                                                                             |                                                                                           
TCAGCTGGGTAATTTTGCTTGCTTTAGCGTTCACTTCAACCCAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGGCTGTA  >  NC_000913/2038872‑2039056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑