Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,030 T→A C824S (TGC→AGC)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,0300TA99.5% 667.1 / ‑5.5 185C824S (TGC→AGC) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (88/96);  minor base G (0/1);  total (88/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 204 total aligned reads displayed.

GCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071940‑2072119
                                                                                          |                                                                                         
gcgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGc                                                                                         >  1:5146853/1‑93 (MQ=255)
 cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCGCGGTCCGGGATGATGCCGGCAGcc                                                                                        >  2:6184349/1‑93 (MQ=255)
 cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        >  1:650340/1‑93 (MQ=255)
  gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       <  2:3342236/93‑1 (MQ=255)
  gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       <  2:3022460/93‑1 (MQ=255)
   ggggTATATGGTGCTTCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTg                                                                                      <  2:1548818/93‑1 (MQ=255)
   ggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTg                                                                                      <  1:4990754/93‑1 (MQ=255)
    gggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTgg                                                                                     <  2:3150911/93‑1 (MQ=255)
      gTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGGAGCCTGGGc                                                                                   >  1:3879842/1‑93 (MQ=255)
      gTATATGGTGCTGCTGGCCATTCTTCCGTTGATCTTAAGGATGATGACGCCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGc                                                                                   <  1:2750415/93‑1 (MQ=255)
       tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg                                                                                  <  2:1857799/93‑1 (MQ=255)
        atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 >  1:431618/1‑93 (MQ=255)
        atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 >  1:896705/1‑93 (MQ=255)
        atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 <  1:2823438/93‑1 (MQ=255)
        atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCCGGGCgg                                                                                 >  2:3332776/1‑93 (MQ=255)
         tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                >  1:3750158/1‑93 (MQ=255)
         tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                >  2:4646364/1‑93 (MQ=255)
         tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                <  1:733848/93‑1 (MQ=255)
             gTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAcc                                                                            >  1:5633988/1‑93 (MQ=255)
              tgctgcTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCt                                                                           <  1:1141430/93‑1 (MQ=255)
               gctgctGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg                                                                          <  1:2452586/93‑1 (MQ=255)
               gctgctGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTg                                                                          >  2:2934696/1‑93 (MQ=255)
                ctgctgTCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                         <  1:1947352/93‑1 (MQ=255)
                ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                         >  2:229252/1‑93 (MQ=255)
                 tgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGaa                                                                        <  1:5699921/93‑1 (MQ=255)
                  gctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAt                                                                       <  2:3634965/93‑1 (MQ=255)
                    tgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCt                                                                     >  2:4498896/1‑93 (MQ=255)
                    tgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCt                                                                     <  2:3423080/93‑1 (MQ=255)
                     ggCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTg                                                                    <  1:1229490/93‑1 (MQ=255)
                        cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTa                                                                 <  1:5434091/93‑1 (MQ=255)
                         aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac                                                                >  2:6118772/1‑93 (MQ=255)
                         aTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTac                                                                >  1:6080262/1‑93 (MQ=255)
                          ttcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca                                                               >  2:4718026/1‑93 (MQ=255)
                            tttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             <  2:4979488/92‑1 (MQ=255)
                            cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             <  1:346218/93‑1 (MQ=255)
                             ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  1:1999863/1‑93 (MQ=255)
                             ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  2:298980/1‑93 (MQ=255)
                              tcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacgcact                                                           >  1:4713866/1‑92 (MQ=255)
                              tcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTTCTCACg                                                           >  2:6013058/1‑93 (MQ=255)
                              tcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACg                                                           >  1:4196100/1‑93 (MQ=255)
                              tcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACg                                                           <  2:760621/93‑1 (MQ=255)
                                cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACggc                                                         >  2:4366909/1‑91 (MQ=255)
                                cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc                                                         <  2:5627176/93‑1 (MQ=255)
                                 gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        <  1:5694673/93‑1 (MQ=255)
                                  ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcct                                                       >  2:6272333/1‑93 (MQ=255)
                                    gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc                                                     <  1:803885/93‑1 (MQ=255)
                                        ttAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGcc                                                 <  1:1642146/93‑1 (MQ=255)
                                           aGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgt                                              <  2:2341019/93‑1 (MQ=255)
                                           aGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgt                                              <  1:2399067/93‑1 (MQ=255)
                                            ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgtg                                             >  1:4922832/1‑93 (MQ=255)
                                             gatgatGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTgg                                            >  1:2078300/1‑93 (MQ=255)
                                               tgatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc                                          >  1:5478372/1‑93 (MQ=255)
                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  1:4579012/93‑1 (MQ=255)
                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         >  1:256658/1‑93 (MQ=255)
                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  1:1688856/93‑1 (MQ=255)
                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         >  2:5259930/1‑93 (MQ=255)
                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  1:3651009/93‑1 (MQ=255)
                                                    aCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACa                                     <  1:2843031/93‑1 (MQ=255)
                                                     cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAt                                    >  2:5390211/1‑93 (MQ=255)
                                                     cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAt                                    <  1:1287398/93‑1 (MQ=255)
                                                       gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  <  1:1884582/93‑1 (MQ=255)
                                                        cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt                                 >  2:3098715/1‑93 (MQ=255)
                                                        cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt                                 <  2:5956381/93‑1 (MQ=255)
                                                        cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt                                 <  2:1747562/93‑1 (MQ=255)
                                                        cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgt                                 <  1:3264930/93‑1 (MQ=255)
                                                          cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg                               >  1:4497612/1‑93 (MQ=255)
                                                          cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg                               <  2:360515/93‑1 (MQ=255)
                                                           ccGTGCCGGCGCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGc                              <  1:2260891/93‑1 (MQ=255)
                                                           ccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGc                              >  2:45228/1‑93 (MQ=255)
                                                            cGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGca                             <  1:5612325/93‑1 (MQ=255)
                                                               gCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAg                          <  2:4144754/93‑1 (MQ=255)
                                                               gCCGGCACGGTCCGCGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAg                          <  1:4817994/93‑1 (MQ=255)
                                                                ccGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg                         >  1:3957063/1‑93 (MQ=255)
                                                                ccGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg                         <  2:5999359/93‑1 (MQ=255)
                                                                ccGGCACGGTCCGGGATGATACCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg                         <  1:3802860/93‑1 (MQ=255)
                                                                 cGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAggg                        >  2:1614165/1‑93 (MQ=255)
                                                                     aCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc                    <  1:2113803/93‑1 (MQ=255)
                                                                     aCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc                    >  1:2874999/1‑93 (MQ=255)
                                                                     aCGGTCCGGGATGATGCAGGCAGCATGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc                    >  2:1992349/1‑93 (MQ=255)
                                                                      cGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc                   <  2:5581573/93‑1 (MQ=255)
                                                                        gTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGc                 >  1:31518/1‑93 (MQ=255)
                                                                        gTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGc                 >  1:6162054/1‑93 (MQ=255)
                                                                        gTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGc                 >  2:2633118/1‑93 (MQ=255)
                                                                         tCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGcc                >  2:3543039/1‑93 (MQ=255)
                                                                         tCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGcc                >  2:6029842/1‑93 (MQ=255)
                                                                         tCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGcc                >  1:2645881/1‑93 (MQ=255)
                                                                         tCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCAGGCCTGTGGGCTGACATTGTGGAACAGGGAACCCGcc                >  2:5721662/1‑93 (MQ=255)
                                                                            gggATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCcaca             <  2:2128804/93‑1 (MQ=255)
                                                                             ggATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAg            >  1:5375609/1‑93 (MQ=255)
                                                                              gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGc           <  2:4787592/93‑1 (MQ=255)
                                                                              gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGc           >  1:4744649/1‑93 (MQ=255)
                                                                              gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGc           >  1:2307663/1‑93 (MQ=255)
                                                                               atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCa          <  1:2997143/93‑1 (MQ=255)
                                                                                tgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAt         <  1:4147875/93‑1 (MQ=255)
                                                                                  atgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGa       <  1:3298244/93‑1 (MQ=255)
                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGaa      >  1:3450279/1‑93 (MQ=255)
                                                                                    gCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGaaa     <  1:992444/93‑1 (MQ=255)
                                                                                     ccGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAg    <  2:1256179/93‑1 (MQ=255)
                                                                                     ccGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAg    <  2:4079225/93‑1 (MQ=255)
                                                                                       ggCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATGGTGGCACAGGGAACCCGCCACAGCATGAAAGCg  <  1:1550079/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071940‑2072119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  NC_000913/2071939‑2072123
                                                                                           |                                                                                             
CGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGC                                                                                             >  SRR4302533.5177955/1‑94 (MQ=60)
 GCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCC                                                                                            >  SRR4302533.654179/1‑94 (MQ=60)
  CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCT                                                                                           <  SRR4302533.4316395/94‑1 (MQ=60)
  CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCT                                                                                           >  SRR4302533.5403394/1‑94 (MQ=60)
   GGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTG                                                                                          <  SRR4302533.475410/94‑1 (MQ=60)
   GGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTG                                                                                          >  SRR4302533.6144759/1‑94 (MQ=60)
    GGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGG                                                                                         <  SRR4302533.5021013/94‑1 (MQ=60)
     GGGTATATGGTGCTGCTGGCCCTTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGGTGATGCCGGGAGCCTGGG                                                                                        >  SRR4302533.5838115/1‑94 (MQ=60)
      GGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGGAGCCTGGGC                                                                                       >  SRR4302533.3903516/1‑94 (MQ=60)
      GGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGC                                                                                       >  SRR4302533.3818270/1‑94 (MQ=60)
       GTATATGGTGCTGCTGGCCATTCTTCCGTTGATCTTAAGGATGATGACGCCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCG                                                                                      <  SRR4302533.2767274/94‑1 (MQ=60)
        TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG                                                                                     <  SRR4302533.4948928/94‑1 (MQ=60)
        TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG                                                                                     >  SRR4302533.787223/1‑94 (MQ=60)
        TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG                                                                                     >  SRR4302533.434191/1‑94 (MQ=60)
        TATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG                                                                                     >  SRR4302533.901969/1‑94 (MQ=60)
         ATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGA                                                                                    >  SRR4302533.3773150/1‑94 (MQ=60)
         ATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGA                                                                                    <  SRR4302533.2840811/94‑1 (MQ=60)
          TATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGAT                                                                                   <  SRR4302533.5721080/94‑1 (MQ=60)
          TATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGAT                                                                                   <  SRR4302533.738166/94‑1 (MQ=60)
           ATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATA                                                                                  <  SRR4302533.246138/94‑1 (MQ=60)
            TGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAC                                                                                 >  SRR4302533.165751/1‑94 (MQ=60)
            TGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGATCCCGTGCCGGCACGGGCCGGGATGATGCCGGCAGCCTGGGCGGATAC                                                                                 >  SRR4302533.2829226/1‑94 (MQ=60)
             GGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACC                                                                                >  SRR4302533.5668057/1‑94 (MQ=60)
              GTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCT                                                                               <  SRR4302533.1429858/94‑1 (MQ=60)
              GTGCTGCTGCCCCTTATTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCGCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCT                                                                               <  SRR4302533.1048829/94‑1 (MQ=60)
               TGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTG                                                                              <  SRR4302533.1148231/94‑1 (MQ=60)
                GCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGA                                                                             <  SRR4302533.2467722/94‑1 (MQ=60)
                 CTGCTGTCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAA                                                                            <  SRR4302533.1959224/94‑1 (MQ=60)
                 CTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAA                                                                            >  SRR4302533.2551695/1‑94 (MQ=60)
                 CTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAA                                                                            >  SRR4302533.1255463/1‑94 (MQ=60)
                  TGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAT                                                                           <  SRR4302533.5734342/94‑1 (MQ=60)
                  TGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAT                                                                           <  SRR4302533.678122/94‑1 (MQ=60)
                    CTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCT                                                                         >  SRR4302533.4733908/1‑94 (MQ=60)
                     TGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGCCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTG                                                                        <  SRR4302533.195590/94‑1 (MQ=60)
                      GGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGG                                                                       <  SRR4302533.1236851/94‑1 (MQ=60)
                      GGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGG                                                                       >  SRR4302533.2106770/1‑94 (MQ=60)
                       GCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGT                                                                      >  SRR4302533.81217/1‑94 (MQ=60)
                         CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTAC                                                                    <  SRR4302533.2377954/94‑1 (MQ=60)
                         CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTAC                                                                    >  SRR4302533.6117033/1‑94 (MQ=60)
                         CATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTAC                                                                    <  SRR4302533.5466816/94‑1 (MQ=60)
                             CTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                >  SRR4302533.2012171/1‑94 (MQ=60)
                             CTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACAC                                                                <  SRR4302533.348330/94‑1 (MQ=60)
                              TTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACgcact                                                               >  SRR4302533.4742436/1‑89 (MQ=60)
                              TTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACG                                                               >  SRR4302533.452310/1‑94 (MQ=60)
                              TTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACG                                                               >  SRR4302533.4221502/1‑94 (MQ=60)
                               TCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCGGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGT                                                              <  SRR4302533.4191643/94‑1 (MQ=60)
                                 CGTTAATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCC                                                            <  SRR4302533.649213/94‑1 (MQ=60)
                                  GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           >  SRR4302533.3840585/1‑94 (MQ=60)
                                  GTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCT                                                           <  SRR4302533.5729063/94‑1 (MQ=60)
                                     GATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCG                                                        >  SRR4302533.3300902/1‑94 (MQ=60)
                                     GATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCG                                                        <  SRR4302533.808606/94‑1 (MQ=60)
                                      ATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGG                                                       >  SRR4302533.3192391/1‑94 (MQ=60)
                                         TTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCT                                                    <  SRR4302533.1652094/94‑1 (MQ=60)
                                         TTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCT                                                    >  SRR4302533.2592278/1‑94 (MQ=60)
                                            AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTG                                                 <  SRR4302533.2413880/94‑1 (MQ=60)
                                            AGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTG                                                 >  SRR4302533.4952655/1‑94 (MQ=60)
                                             GGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGG                                                <  SRR4302533.1987785/94‑1 (MQ=60)
                                             GGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGG                                                >  SRR4302533.2091092/1‑94 (MQ=60)
                                             GGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGG                                                <  SRR4302533.2300586/94‑1 (MQ=60)
                                               ATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGC                                              >  SRR4302533.5511398/1‑94 (MQ=60)
                                               ATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGC                                              >  SRR4302533.4108065/1‑94 (MQ=60)
                                                TGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCT                                             >  SRR4302533.258223/1‑94 (MQ=60)
                                                TGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCT                                             <  SRR4302533.1808461/94‑1 (MQ=60)
                                                 GATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTG                                            <  SRR4302533.3673457/94‑1 (MQ=60)
                                                 GATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTG                                            <  SRR4302533.4606763/94‑1 (MQ=60)
                                                 GATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTG                                            <  SRR4302533.1699089/94‑1 (MQ=60)
                                                  ATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGA                                           >  SRR4302533.3456840/1‑94 (MQ=60)
                                                   TGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGAC                                          <  SRR4302533.2063377/94‑1 (MQ=60)
                                                   TGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGAC                                          <  SRR4302533.5589405/94‑1 (MQ=60)
                                                    GACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGACTGGGCGGATACCCGAATCTGGGACACACGTCCCCCGCCCCGTGGGCTGACA                                         >  SRR4302533.93324/1‑94 (MQ=60)
                                                     ACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAT                                        <  SRR4302533.2860527/94‑1 (MQ=60)
                                                     ACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAT                                        <  SRR4302533.4954831/94‑1 (MQ=60)
                                                      CGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATT                                       <  SRR4302533.1295144/94‑1 (MQ=60)
                                                       GGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGTCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTG                                      <  SRR4302533.2749780/94‑1 (MQ=60)
                                                        GCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGT                                     >  SRR4302533.1178311/1‑94 (MQ=60)
                                                        GCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGT                                     <  SRR4302533.1896091/94‑1 (MQ=60)
                                                         CTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTG                                    >  SRR4302533.3109552/1‑94 (MQ=60)
                                                         CTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTG                                    <  SRR4302533.3285075/94‑1 (MQ=60)
                                                         CTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTG                                    <  SRR4302533.4544537/94‑1 (MQ=60)
                                                          TCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGG                                   >  SRR4302533.4524849/1‑94 (MQ=60)
                                                           CCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGC                                  >  SRR4302533.2890997/1‑94 (MQ=60)
                                                           CCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGC                                  <  SRR4302533.3609860/94‑1 (MQ=60)
                                                           CCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGC                                  <  SRR4302533.36962/94‑1 (MQ=60)
                                                            CCGTGCCGGCGCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCA                                 <  SRR4302533.2274876/94‑1 (MQ=60)
                                                            CCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCA                                 <  SRR4302533.3472182/94‑1 (MQ=60)
                                                            CCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCA                                 >  SRR4302533.5665374/1‑94 (MQ=60)
                                                             CGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCAC                                <  SRR4302533.5646248/94‑1 (MQ=60)
                                                              GTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACA                               >  SRR4302533.5139589/1‑94 (MQ=60)
                                                                GCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGG                             >  SRR4302533.3981182/1‑94 (MQ=60)
                                                                GCCGGCACGGTCCGCGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGG                             <  SRR4302533.4847197/94‑1 (MQ=60)
                                                                 CCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGG                            <  SRR4302533.5461475/94‑1 (MQ=60)
                                                                 CCGGCACGGTCCGGGATGATACCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGG                            <  SRR4302533.3826129/94‑1 (MQ=60)
                                                                  CGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGA                           <  SRR4302533.5784111/94‑1 (MQ=60)
                                                                    GCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAC                         <  SRR4302533.5499399/94‑1 (MQ=60)
                                                                     CACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACC                        >  SRR4302533.2892689/1‑94 (MQ=60)
                                                                      ACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCC                       <  SRR4302533.284128/94‑1 (MQ=60)
                                                                      ACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCC                       <  SRR4302533.2126838/94‑1 (MQ=60)
                                                                       CGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCG                      >  SRR4302533.2915850/1‑94 (MQ=60)
                                                                        GGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGC                     >  SRR4302533.3976077/1‑94 (MQ=60)
                                                                        GGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGC                     >  SRR4302533.6199106/1‑94 (MQ=60)
                                                                        GGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGC                     >  SRR4302533.31735/1‑94 (MQ=60)
                                                                         GTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCC                    >  SRR4302533.2662140/1‑94 (MQ=60)
                                                                           CCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCAC                  <  SRR4302533.916177/94‑1 (MQ=60)
                                                                           CCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCAC                  <  SRR4302533.2237025/94‑1 (MQ=60)
                                                                             GGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAG                >  SRR4302533.5407915/1‑94 (MQ=60)
                                                                              GGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGC               >  SRR4302533.2321957/1‑94 (MQ=60)
                                                                              GGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGC               >  SRR4302533.4773416/1‑94 (MQ=60)
                                                                              GGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGC               >  SRR4302533.4773415/1‑94 (MQ=60)
                                                                                ATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAT             <  SRR4302533.3015470/94‑1 (MQ=60)
                                                                                 TGATGCCGGCAGCGTGGGCGGATCCCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATG            <  SRR4302533.5732382/94‑1 (MQ=60)
                                                                                 TGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATG            <  SRR4302533.4173005/94‑1 (MQ=60)
                                                                                   ATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAA          <  SRR4302533.3318599/94‑1 (MQ=60)
                                                                                   ATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAA          >  SRR4302533.3471584/1‑94 (MQ=60)
                                                                                     GCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAG        <  SRR4302533.998246/94‑1 (MQ=60)
                                                                                       CGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG      <  SRR4302533.1630833/94‑1 (MQ=60)
                                                                                        GGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGT     <  SRR4302533.3356768/94‑1 (MQ=60)
                                                                                        GGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGT     >  SRR4302533.1322012/1‑94 (MQ=60)
                                                                                        GGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATGGTGGCACAGGGAACCCGCCACAGCATGAAAGCGT     <  SRR4302533.1559419/94‑1 (MQ=60)
                                                                                         GCAGCGTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTC    <  SRR4302533.725026/94‑1 (MQ=60)
                                                                                         GCAGCCTGGGCGGATACCTGAATCTGGTACACCCGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTC    <  SRR4302533.4651784/94‑1 (MQ=60)
                                                                                         GCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTC    >  SRR4302533.1599613/1‑94 (MQ=60)
                                                                                         GCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTC    >  SRR4302533.2742230/1‑94 (MQ=60)
                                                                                           AGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  SRR4302533.2981177/1‑94 (MQ=60)
                                                                                           AGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  SRR4302533.922969/1‑94 (MQ=60)
                                                                                           |                                                                                             
CGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCAT  >  NC_000913/2071939‑2072123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑