Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC100.0% 751.2 / NA 230T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (124/106);  total (124/106)

Only 100 of 237 total aligned reads displayed.

CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163
                                                                                          |                                                                                         
 aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        <  2:3803217/93‑1 (MQ=255)
 aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        >  2:1220756/1‑93 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAc                                                                                       <  1:947114/93‑1 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTCGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAc                                                                                       <  1:4439179/93‑1 (MQ=255)
   aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      <  2:4249343/93‑1 (MQ=255)
    gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     >  2:6163843/1‑93 (MQ=255)
     cTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGc                                                                                    <  1:2342303/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:5896969/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  1:4533839/1‑93 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  2:3353551/1‑93 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  2:1594260/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  2:861921/1‑93 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  2:5237688/1‑93 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  2:1356817/1‑93 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                <  2:3159354/93‑1 (MQ=255)
          cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               >  2:2715493/1‑93 (MQ=255)
          cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               <  2:5676155/93‑1 (MQ=255)
          cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               >  2:6276802/1‑93 (MQ=255)
           aGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCggg                                                                              >  2:2125417/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGGAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  2:4525403/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  1:3781651/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGCGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            <  2:5135519/93‑1 (MQ=255)
              tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGata                                                                           >  2:708483/1‑93 (MQ=255)
               cGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGatat                                                                          >  2:354841/1‑93 (MQ=255)
                gTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtt                                                                         <  1:1163683/93‑1 (MQ=255)
                 ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        >  2:1983558/1‑93 (MQ=255)
                  tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       >  2:3496872/1‑93 (MQ=255)
                    aaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGc                                                                     <  1:5894287/93‑1 (MQ=255)
                     attACGTCACGCGCATGGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  2:2560915/91‑1 (MQ=255)
                         cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                <  1:6227671/93‑1 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  2:445583/93‑1 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:1552225/93‑1 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:1377997/93‑1 (MQ=255)
                           ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              >  2:4366315/1‑93 (MQ=255)
                           ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              >  1:1073027/1‑93 (MQ=255)
                            cACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgc                                                             >  1:2542899/1‑93 (MQ=255)
                             aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  2:2620072/1‑93 (MQ=255)
                               gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  1:6189749/93‑1 (MQ=255)
                                 gcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCtt                                                        <  2:4313338/93‑1 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:2176121/1‑93 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:3505351/1‑93 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               <  2:1613118/93‑1 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               <  2:3248281/93‑1 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               <  1:193219/93‑1 (MQ=255)
                                           gtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACag                                              >  1:1759851/1‑93 (MQ=255)
                                           gtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACag                                              <  1:5608272/93‑1 (MQ=255)
                                            taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             <  1:1022910/93‑1 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  2:2808017/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  1:717265/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            <  2:2486647/93‑1 (MQ=255)
                                             aggtGTTTGAAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            <  2:2276016/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  1:1836958/1‑93 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  1:4029009/1‑93 (MQ=255)
                                                tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  1:965427/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:1884465/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:3012535/1‑93 (MQ=255)
                                                   ttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGa                                      >  1:51212/1‑93 (MQ=255)
                                                    tGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaa                                     <  2:4414165/93‑1 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  1:5068111/93‑1 (MQ=255)
                                                        aaaaGTTTAATCGCTTCTGTTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGt                                 >  2:6114275/1‑93 (MQ=255)
                                                        aaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGt                                 >  2:3917523/1‑93 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  1:3142002/93‑1 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  1:846886/93‑1 (MQ=255)
                                                            gTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTg                             <  1:5243838/93‑1 (MQ=255)
                                                              ttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcg                           >  2:6057313/1‑93 (MQ=255)
                                                               tAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcgc                          >  1:15243/1‑93 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        >  1:2687386/1‑93 (MQ=255)
                                                                  tCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGtt                       >  2:6220270/1‑93 (MQ=255)
                                                                   cGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTc                      <  1:286578/93‑1 (MQ=255)
                                                                   cGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTc                      <  1:6044738/93‑1 (MQ=255)
                                                                   cGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTc                      <  1:1733324/93‑1 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     >  2:317088/1‑93 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     >  2:239821/1‑93 (MQ=255)
                                                                     cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    <  2:4804857/93‑1 (MQ=255)
                                                                     cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    <  2:5521594/93‑1 (MQ=255)
                                                                     cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  2:3091372/1‑93 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:2555076/1‑93 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  1:4317448/1‑93 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  1:580389/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  <  1:1648270/93‑1 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  2:1397319/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  2:5385568/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  2:2855025/1‑93 (MQ=255)
                                                                        cTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAc                 <  1:2934148/93‑1 (MQ=255)
                                                                            ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             <  1:427555/93‑1 (MQ=255)
                                                                             tCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCa            <  1:2718368/93‑1 (MQ=255)
                                                                             tCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCa            >  1:5452515/1‑93 (MQ=255)
                                                                               aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          >  2:4754827/1‑93 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  2:5117542/93‑1 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  2:576941/93‑1 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       <  1:4934545/93‑1 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       <  1:3962004/93‑1 (MQ=255)
                                                                                   gAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATg      <  2:917989/93‑1 (MQ=255)
                                                                                   gAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATg      <  1:782801/93‑1 (MQ=255)
                                                                                   gAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATg      >  1:2290760/1‑93 (MQ=255)
                                                                                   gAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATg      <  1:4760681/93‑1 (MQ=255)
                                                                                     gTCGGCAAACAGAATAGGGATATTTTGCTTGATAGCGGCTTCCTGTAACAGAACACCGAAACGTTTTGCGCGTTCTGAACGCTCAACGATTAc    >  2:3424167/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGCGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   <  1:3387294/93‑1 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   <  1:2136390/93‑1 (MQ=255)
                                                                                       cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCATCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  >  1:4178386/1‑93 (MQ=255)
                                                                                          |                                                                                         
CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302533.2882095/1‑94 (MQ=60)
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302533.1189845/1‑94 (MQ=60)
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302533.3976972/1‑94 (MQ=60)
 TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                              >  SRR4302533.4202483/1‑94 (MQ=60)
 TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                              <  SRR4302533.5914557/94‑1 (MQ=60)
 TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                              >  SRR4302533.2296430/1‑94 (MQ=60)
  GCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAA                                                                                             >  SRR4302533.280064/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302533.2432470/1‑94 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302533.952682/94‑1 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTCGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302533.4466013/94‑1 (MQ=60)
      AGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                         >  SRR4302533.1148604/1‑94 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302533.4561291/1‑94 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302533.4307791/1‑94 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       <  SRR4302533.2356805/94‑1 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      <  SRR4302533.4744357/94‑1 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      <  SRR4302533.4021559/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302533.6029666/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302533.2482671/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302533.3087193/94‑1 (MQ=60)
            CCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGG                                                                                   >  SRR4302533.3824811/1‑94 (MQ=60)
              AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                 >  SRR4302533.569762/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                >  SRR4302533.3804813/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                <  SRR4302533.2907842/94‑1 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                <  SRR4302533.957695/94‑1 (MQ=60)
                TTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATA                                                                               <  SRR4302533.4252348/94‑1 (MQ=60)
                 TCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAT                                                                              >  SRR4302533.4560364/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302533.600904/1‑94 (MQ=60)
                   GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                            <  SRR4302533.5138212/94‑1 (MQ=60)
                   GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                            <  SRR4302533.1170662/94‑1 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                           <  SRR4302533.3979007/94‑1 (MQ=60)
                     TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                          <  SRR4302533.5227846/94‑1 (MQ=60)
                      AAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGC                                                                         >  SRR4302533.2966445/1‑94 (MQ=60)
                       AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                        <  SRR4302533.5929886/94‑1 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    >  SRR4302533.797663/1‑94 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    <  SRR4302533.1315955/94‑1 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   <  SRR4302533.6264980/94‑1 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  <  SRR4302533.1561571/94‑1 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  >  SRR4302533.1079354/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  <  SRR4302533.1386285/94‑1 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302533.2558547/1‑94 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302533.1622246/1‑94 (MQ=60)
                                CCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGC                                                               <  SRR4302533.1812377/94‑1 (MQ=60)
                                 CGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCC                                                              >  SRR4302533.5534170/1‑94 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302533.6226894/94‑1 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          <  SRR4302533.1053931/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGATTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302533.3405013/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302533.86465/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302533.347958/94‑1 (MQ=60)
                                         GCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTA                                                      <  SRR4302533.2079206/94‑1 (MQ=60)
                                            AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                   >  SRR4302533.2355671/1‑94 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  <  SRR4302533.194437/94‑1 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  >  SRR4302533.1770577/1‑94 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  <  SRR4302533.898298/94‑1 (MQ=60)
                                              GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                 >  SRR4302533.639999/1‑94 (MQ=60)
                                              GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                 <  SRR4302533.5642161/94‑1 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                >  SRR4302533.1536940/1‑94 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                >  SRR4302533.721495/1‑94 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                <  SRR4302533.1028922/94‑1 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302533.4053531/1‑94 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302533.1848161/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302533.971091/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302533.5251382/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302533.1217229/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302533.4951013/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302533.1777068/1‑94 (MQ=60)
                                                     TTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGA                                          >  SRR4302533.51560/1‑94 (MQ=60)
                                                       TGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAA                                        >  SRR4302533.164555/1‑94 (MQ=60)
                                                        GCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       <  SRR4302533.5098716/94‑1 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302533.2583893/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302533.2718422/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302533.507872/1‑94 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302533.5768021/94‑1 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302533.5926522/94‑1 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  <  SRR4302533.851844/94‑1 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  <  SRR4302533.3161317/94‑1 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  <  SRR4302533.1519573/94‑1 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 <  SRR4302533.1694960/94‑1 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 >  SRR4302533.1962237/1‑94 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAAGTTCTG                                 >  SRR4302533.5170157/1‑94 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302533.441754/94‑1 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302533.5275356/94‑1 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302533.4537399/94‑1 (MQ=60)
                                                                 TTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGC                              >  SRR4302533.15350/1‑94 (MQ=60)
                                                                 TTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTACTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGC                              <  SRR4302533.751219/94‑1 (MQ=60)
                                                                   AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                            >  SRR4302533.2177614/1‑94 (MQ=60)
                                                                   AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                            >  SRR4302533.2703862/1‑94 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         <  SRR4302533.288330/94‑1 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         <  SRR4302533.6081357/94‑1 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         <  SRR4302533.1743836/94‑1 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        <  SRR4302533.4090469/94‑1 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302533.4343447/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302533.583805/1‑94 (MQ=60)
                                                                          TCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAC                     <  SRR4302533.1658255/94‑1 (MQ=60)
                                                                           CTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACG                    <  SRR4302533.2952088/94‑1 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302533.6244077/1‑94 (MQ=60)
                                                                              CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                 >  SRR4302533.1744750/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                <  SRR4302533.430107/94‑1 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                >  SRR4302533.5485359/1‑94 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               <  SRR4302533.5286987/94‑1 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               <  SRR4302533.2735056/94‑1 (MQ=60)
                                                                                  AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG             <  SRR4302533.2737434/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302533.3986143/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302533.4964424/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          >  SRR4302533.2304947/1‑94 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          >  SRR4302533.1857330/1‑94 (MQ=60)
                                                                                      GAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGA         <  SRR4302533.4789563/94‑1 (MQ=60)
                                                                                      GAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGA         <  SRR4302533.787404/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       >  SRR4302533.643560/1‑94 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGCGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      <  SRR4302533.3408211/94‑1 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCATCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      >  SRR4302533.4203700/1‑94 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      <  SRR4302533.2149563/94‑1 (MQ=60)
                                                                                          CGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAAT     <  SRR4302533.4796519/94‑1 (MQ=60)
                                                                                          CGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAAT     <  SRR4302533.6090359/94‑1 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302533.3020850/1‑94 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   <  SRR4302533.6340640/94‑1 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   <  SRR4302533.246505/94‑1 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  <  SRR4302533.12426/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑