Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC99.8% 1348.7 / ‑5.0 417T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (236/180);  total (236/181)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 422 total aligned reads displayed.

CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163
                                                                                          |                                                                                         
cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaa                                                                                         >  1:8015351/1‑93 (MQ=255)
cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaa                                                                                         >  2:7428949/1‑93 (MQ=255)
 aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        <  1:7336112/93‑1 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAAc                                                                                       >  1:5882497/1‑93 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAAc                                                                                       >  1:4857259/1‑93 (MQ=255)
   aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      >  2:3095220/1‑93 (MQ=255)
    gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     >  2:2837082/1‑93 (MQ=255)
    gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     <  1:2451961/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  1:1297179/1‑93 (MQ=255)
      caTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:4655166/92‑1 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  1:2009220/1‑93 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  <  1:7765585/93‑1 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 >  1:2756321/1‑93 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:2622218/93‑1 (MQ=255)
        tCCAGGTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:886521/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  1:325486/1‑93 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                <  2:8237178/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  2:4751815/1‑93 (MQ=255)
           aGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGATTAATCGCTTCTGCTTCAGTGGATTCGGCAAACAGCATCggg                                                                              >  1:9431869/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  1:3269791/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             <  1:1380992/93‑1 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  1:876622/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  2:7933449/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  2:9642100/1‑93 (MQ=255)
                 ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATAttt                                                                        <  1:8268998/93‑1 (MQ=255)
                 ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        <  1:7014998/93‑1 (MQ=255)
                     aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  1:9981003/93‑1 (MQ=255)
                     aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    >  1:6495158/1‑93 (MQ=255)
                      gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  2:8060778/93‑1 (MQ=255)
                       tACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTa                                                                  >  1:6082841/1‑93 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  2:8488995/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  2:3456021/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  1:8414599/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 >  2:1122265/1‑93 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:7209952/93‑1 (MQ=255)
                           ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              >  1:9513083/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGTCAAACAGCATCGGGATATTTTGCTTAATTgcgc                                                           >  1:9668672/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCgcgc                                                           >  2:3006705/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTATACAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCgcgc                                                           <  2:2015412/93‑1 (MQ=255)
                               gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  1:5350538/93‑1 (MQ=255)
                                cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         <  2:452217/93‑1 (MQ=255)
                                cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         >  2:4868705/1‑93 (MQ=255)
                                  cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       >  1:1555031/1‑93 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      <  2:7241740/93‑1 (MQ=255)
                                        cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 >  2:4241653/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  2:3576085/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  2:5677014/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  1:4044545/1‑93 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               >  2:3869757/1‑93 (MQ=255)
                                           gtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACag                                              >  2:9070673/1‑93 (MQ=255)
                                            taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             >  2:5478484/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  1:7441859/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            <  2:5896828/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:489796/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:751920/93‑1 (MQ=255)
                                               gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCACACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGGAACCGagca                                          >  1:3624296/1‑93 (MQ=255)
                                                tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  2:1699948/1‑93 (MQ=255)
                                                tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         <  1:1028829/93‑1 (MQ=255)
                                                 gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  1:1808171/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGGGCGGGCAACCAGAATCGGGATATTTGGCTTATTCGAGCCTTCCTGTAACAGggcagca                                       >  1:5110726/1‑92 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:2288266/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:6909253/1‑93 (MQ=255)
                                                    tGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaa                                     >  1:4148242/1‑93 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  2:657098/93‑1 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  2:8894664/93‑1 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  1:5706087/93‑1 (MQ=255)
                                                      cAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAc                                   <  2:142679/93‑1 (MQ=255)
                                                        aaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGt                                 >  2:9808868/1‑93 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:7345754/93‑1 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:1833234/93‑1 (MQ=255)
                                                           aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              >  1:9891203/1‑93 (MQ=255)
                                                            gTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTg                             >  1:3820233/1‑93 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            <  1:4382341/93‑1 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            <  1:9241005/93‑1 (MQ=255)
                                                               tAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcgc                          >  2:3665989/1‑93 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        <  2:6444676/93‑1 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        <  1:6807519/93‑1 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     <  2:2610020/93‑1 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGcgcg                         >  1:1902497/1‑87 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCATTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:5323431/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  1:9719660/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  <  1:2891430/93‑1 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  1:6187180/1‑93 (MQ=255)
                                                                         tGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACg                >  2:9510017/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  2:9266807/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              <  2:3210748/93‑1 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  1:5452916/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  2:5145061/1‑93 (MQ=255)
                                                                            ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             >  1:6643429/1‑93 (MQ=255)
                                                                             tCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCa            >  1:1703391/1‑93 (MQ=255)
                                                                               aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          >  2:7738/1‑93 (MQ=255)
                                                                                gTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCg         >  1:8613717/1‑93 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  1:4638261/93‑1 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  2:6150364/93‑1 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       >  2:1299166/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCCCCGATGACa   >  1:9000207/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  2:8656896/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  1:2254566/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCGCagaa         >  1:2170163/1‑84 (MQ=255)
                                                                                       cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:8393132/93‑1 (MQ=255)
                                                                                          |                                                                                         
CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 232 total aligned reads displayed.
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302540.2688353/1‑94 (MQ=60)
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302540.5187392/1‑94 (MQ=60)
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302540.6800426/1‑94 (MQ=60)
 TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                              >  SRR4302540.2369371/1‑94 (MQ=60)
  GCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAA                                                                                             >  SRR4302540.8108583/1‑94 (MQ=60)
   CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAA                                                                                            >  SRR4302540.7104049/1‑94 (MQ=60)
   CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAA                                                                                            >  SRR4302540.2686995/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           <  SRR4302540.7417987/94‑1 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302540.19767/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302540.6675574/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302540.3174046/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           <  SRR4302540.2539784/94‑1 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAAC                                                                                           >  SRR4302540.4909332/1‑94 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302540.4064533/94‑1 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302540.4064532/94‑1 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302540.9796299/94‑1 (MQ=60)
       GTTATNCNGTNCGTTAAAGTANGNCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        <  SRR4302540.8924387/94‑1 (MQ=60)
       GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        <  SRR4302540.2478360/94‑1 (MQ=60)
       GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        <  SRR4302540.10015639/94‑1 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302540.1310616/1‑94 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302540.2475352/1‑94 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       <  SRR4302540.4616118/94‑1 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302540.8700602/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302540.6153129/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302540.2030952/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGAAGGTGGTTGCAAAAAGTTTATTCGCTTCTGCTTCAGTGGAGTCGGCAAACAtcct                                                                                      >  SRR4302540.4351748/1‑90 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302540.7856367/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302540.2786108/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302540.2338494/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302540.2217591/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302540.5758364/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302540.4335202/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302540.8519849/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302540.10002713/1‑94 (MQ=60)
           TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    >  SRR4302540.1508362/1‑94 (MQ=60)
           TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    <  SRR4302540.2650567/94‑1 (MQ=60)
           TCCAGGTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    <  SRR4302540.895928/94‑1 (MQ=60)
            CCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGG                                                                                   >  SRR4302540.7771411/1‑94 (MQ=60)
             CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                  >  SRR4302540.2076411/1‑94 (MQ=60)
             CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                  >  SRR4302540.1286819/1‑94 (MQ=60)
             CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                  <  SRR4302540.8990924/94‑1 (MQ=60)
              AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                 >  SRR4302540.3305478/1‑94 (MQ=60)
              AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                 >  SRR4302540.7486572/1‑94 (MQ=60)
              AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                 >  SRR4302540.885930/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                <  SRR4302540.1395729/94‑1 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                >  SRR4302540.877465/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                >  SRR4302540.7968189/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                >  SRR4302540.4876538/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302540.7506446/1‑94 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTT                                                                           <  SRR4302540.8365076/94‑1 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                           >  SRR4302540.1332760/1‑94 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                           <  SRR4302540.7092892/94‑1 (MQ=60)
                     TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                          <  SRR4302540.7364001/94‑1 (MQ=60)
                       AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                        >  SRR4302540.2377053/1‑94 (MQ=60)
                       AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                        >  SRR4302540.6567389/1‑94 (MQ=60)
                        AGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTT                                                                       <  SRR4302540.4274373/94‑1 (MQ=60)
                        AGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTT                                                                       <  SRR4302540.10097386/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302540.369966/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      >  SRR4302540.6150531/1‑94 (MQ=60)
                         ATACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302540.5091148/94‑1 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATCTTTTGCTTAAT                                                                    >  SRR4302540.9868725/1‑94 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    <  SRR4302540.5985265/94‑1 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    <  SRR4302540.8512269/94‑1 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    <  SRR4302540.8770757/94‑1 (MQ=60)
                            CGCCACTCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   <  SRR4302540.1344744/94‑1 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   <  SRR4302540.2782253/94‑1 (MQ=60)
                            CGCCACGAGCATCGCCAGGGAGGTGTTTGCAAAAAGTTTAATCGCTTCTTCTTCAGTTTAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302540.7176372/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  >  SRR4302540.9815958/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  <  SRR4302540.7290330/94‑1 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  >  SRR4302540.9625767/1‑94 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302540.3303026/1‑94 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302540.6387362/1‑94 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGATTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTCATCGG                                                                 >  SRR4302540.6387365/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302540.6865822/1‑94 (MQ=60)
                                CCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGC                                                               <  SRR4302540.3848020/94‑1 (MQ=60)
                                ACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGTCAAACAGCATCGGGATATTTTGCTTAATTGCGC                                                               >  SRR4302540.9782472/1‑94 (MQ=60)
                                ACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGC                                                               >  SRR4302540.5994907/1‑94 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCCAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             >  SRR4302540.2472938/1‑94 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302540.5409255/94‑1 (MQ=60)
                                    GCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTC                                                           >  SRR4302540.1572134/1‑94 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          >  SRR4302540.9555736/1‑94 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          >  SRR4302540.9555737/1‑94 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          <  SRR4302540.7568831/94‑1 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCACTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          <  SRR4302540.10074907/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302540.5924677/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302540.6918476/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302540.4147909/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302540.1810608/94‑1 (MQ=60)
                                         GCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTA                                                      >  SRR4302540.4728309/1‑94 (MQ=60)
                                          CCAGGTAGGTTCTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     <  SRR4302540.1760334/94‑1 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     <  SRR4302540.1760335/94‑1 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    >  SRR4302540.4088152/1‑94 (MQ=60)
                                            AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                   >  SRR4302540.8617198/1‑94 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  >  SRR4302540.6646542/1‑94 (MQ=60)
                                             GGTAGGTGCTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  <  SRR4302540.8446160/94‑1 (MQ=60)
                                              GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                 >  SRR4302540.5163329/1‑94 (MQ=60)
                                              GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                 <  SRR4302540.5447106/94‑1 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                <  SRR4302540.1496745/94‑1 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                >  SRR4302540.3734574/1‑94 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                >  SRR4302540.202994/1‑94 (MQ=60)
                                               TAGGTGTTGGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTCAATCGCGCCTTCCTGTAACAGAG                                                <  SRR4302540.1856728/94‑1 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302540.9414499/1‑94 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302540.9719894/1‑94 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               <  SRR4302540.7274937/94‑1 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302540.8115733/1‑94 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCACACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGGAACCGAGCA                                              >  SRR4302540.3663959/1‑94 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              >  SRR4302540.635570/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302540.9174045/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302540.3295109/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            <  SRR4302540.1039265/94‑1 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302540.1827870/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302540.6099484/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAACAGCATCGGGATATTTTGCTTAATggagctttcatgtaaaagagcaga                                            >  SRR4302540.3295103/1‑70 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGGGCGGGCAACCAGAATCGGGATATTTGGCTTATTCGAGCCTTCCTGTAACAGGGCAGCA                                           >  SRR4302540.5166041/1‑94 (MQ=40)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302540.5133136/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302540.6991868/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302540.1214460/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           <  SRR4302540.7794446/94‑1 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302540.1663832/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           <  SRR4302540.2023631/94‑1 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           <  SRR4302540.1835261/94‑1 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTGCCTGTCACAGAGCgccg                                           >  SRR4302540.1663831/1‑90 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGAGCCTTCCTGTAACAGAGCAGCG                                           <  SRR4302540.1835263/94‑1 (MQ=60)
                                                        GCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       <  SRR4302540.5768908/94‑1 (MQ=60)
                                                        GCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       <  SRR4302540.4860603/94‑1 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                      <  SRR4302540.6698823/94‑1 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                      >  SRR4302540.2923964/1‑94 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACACAGCAGCGAAACG                                      >  SRR4302540.7416460/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302540.8968143/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302540.4138621/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302540.6119283/1‑94 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302540.93984/94‑1 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302540.8875077/94‑1 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302540.531903/94‑1 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302540.429220/94‑1 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302540.5358875/1‑94 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302540.10006691/1‑94 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302540.10006694/1‑94 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302540.7877445/1‑94 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 >  SRR4302540.3861814/1‑94 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302540.8110839/94‑1 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302540.8986708/94‑1 (MQ=60)
                                                               ATTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302540.147643/94‑1 (MQ=60)
                                                                TTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCG                               <  SRR4302540.1777713/94‑1 (MQ=60)
                                                                TTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCG                               <  SRR4302540.9351691/94‑1 (MQ=60)
                                                                TTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCG                               <  SRR4302540.4429929/94‑1 (MQ=60)
                                                                 TTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGC                              <  SRR4302540.9248982/94‑1 (MQ=60)
                                                                  TAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCG                             <  SRR4302540.2493432/94‑1 (MQ=60)
                                                                   AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                            >  SRR4302540.2111537/1‑94 (MQ=60)
                                                                   AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                            <  SRR4302540.5750918/94‑1 (MQ=60)
                                                                   AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                            >  SRR4302540.2684408/1‑94 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                           <  SRR4302540.8271190/94‑1 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                           <  SRR4302540.2903042/94‑1 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                           >  SRR4302540.10091128/1‑94 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                           <  SRR4302540.6882850/94‑1 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGCTCTGCGCGTT                           >  SRR4302540.4181285/1‑94 (MQ=60)
                                                                     TCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTC                          >  SRR4302540.1156463/1‑94 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302540.4450282/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTaagcg                       >  SRR4302540.1923300/1‑89 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       <  SRR4302540.8799736/94‑1 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302540.6881542/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302540.5459842/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302540.719073/1‑94 (MQ=60)
                                                                         TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAC                      >  SRR4302540.9561683/1‑94 (MQ=60)
                                                                         TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAA                      >  SRR4302540.9833851/1‑94 (MQ=60)
                                                                         TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAA                      >  SRR4302540.6256078/1‑94 (MQ=60)
                                                                          TCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAC                     <  SRR4302540.5837965/94‑1 (MQ=60)
                                                                          TCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAC                     <  SRR4302540.3331536/94‑1 (MQ=60)
                                                                          TCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAC                     <  SRR4302540.2922942/94‑1 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302540.7423279/1‑94 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302540.9410676/1‑94 (MQ=60)
                                                                             GCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                  >  SRR4302540.5512930/1‑94 (MQ=60)
                                                                             GCCTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                  <  SRR4302540.836654/94‑1 (MQ=60)
                                                                              CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACATAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                 >  SRR4302540.9785350/1‑94 (MQ=60)
                                                                              CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                 >  SRR4302540.6716938/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                >  SRR4302540.1721819/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                <  SRR4302540.30146/94‑1 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               <  SRR4302540.9693968/94‑1 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               <  SRR4302540.2248643/94‑1 (MQ=60)
                                                                                  AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG             >  SRR4302540.8676826/1‑94 (MQ=60)
                                                                                  AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG             >  SRR4302540.7548277/1‑94 (MQ=60)
                                                                                   GTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGA            <  SRR4302540.6192703/94‑1 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT           <  SRR4302540.3901800/94‑1 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT           <  SRR4302540.4687922/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302540.6815644/94‑1 (MQ=60)
                                                                                       AGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGAC        <  SRR4302540.4101262/94‑1 (MQ=60)
                                                                                       AGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGAC        >  SRR4302540.3169469/1‑94 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCgcagaacaagcg       >  SRR4302540.2193567/1‑82 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCCCCGATGACA       >  SRR4302540.9108188/1‑94 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       <  SRR4302540.9533403/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       >  SRR4302540.2856913/1‑94 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       <  SRR4302540.5442569/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       >  SRR4302540.2278760/1‑94 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      <  SRR4302540.3399379/94‑1 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      >  SRR4302540.2568910/1‑94 (MQ=60)
                                                                                          CGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAAT     <  SRR4302540.5841759/94‑1 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302540.617466/1‑94 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   <  SRR4302540.7044609/94‑1 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  <  SRR4302540.9506555/94‑1 (MQ=60)
                                                                                             AAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  <  SRR4302540.5251584/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑