Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108,194	T→C	Q283R (CAG→CGG)	rfbA ←	glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108,194	0	T	C	99.5%	654.5 / ‑4.4	201	Q283R (CAG→CGG)	rfbA	glucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (103/97); minor base A (0/1); total (103/98)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

Only 100 of 203 total aligned reads displayed.

AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283

   tACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCa                                                                                      <  1:4301/93‑1 (MQ=255)

     ctctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATa                                                                                    <  2:4961202/93‑1 (MQ=255)

      tctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATaa                                                                                   >  1:2290603/1‑93 (MQ=255)

      tctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATaa                                                                                   >  1:220699/1‑93 (MQ=255)

       ctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAat                                                                                  <  1:3147706/93‑1 (MQ=255)

       ctTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAat                                                                                  >  1:371812/1‑93 (MQ=255)

        ttGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAatt                                                                                 >  1:429475/1‑93 (MQ=255)

        ttGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAatt                                                                                 <  2:6182304/93‑1 (MQ=255)

        ttGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAatt                                                                                 >  2:1834108/1‑93 (MQ=255)

        ttGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACAGCCCATAatt                                                                                 <  2:1989473/93‑1 (MQ=255)

         tGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAatta                                                                                >  2:5383808/1‑93 (MQ=255)

         tGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAatta                                                                                <  1:5907924/93‑1 (MQ=255)

            cTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTc                                                                             <  2:3052968/93‑1 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            >  1:4249920/1‑93 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  2:1702070/93‑1 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  1:5131041/93‑1 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            >  1:1000322/1‑93 (MQ=255)

              ccAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCtt                                                                           >  2:5285797/1‑93 (MQ=255)

              ccAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCtt                                                                           <  2:2149262/93‑1 (MQ=255)

              ccAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCtt                                                                           >  2:4925418/1‑93 (MQ=255)

               cAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCttt                                                                          >  1:4424078/1‑93 (MQ=255)

                aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         >  2:3568054/1‑93 (MQ=255)

                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        <  1:2926213/93‑1 (MQ=255)

                  aaTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  1:1937758/1‑93 (MQ=255)

                    ttAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAg                                                                     >  2:1840658/1‑93 (MQ=255)

                    ttAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAg                                                                     <  1:2184090/93‑1 (MQ=255)

                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    >  2:2441060/1‑93 (MQ=255)

                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    <  2:5911330/93‑1 (MQ=255)

                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    >  1:785414/1‑93 (MQ=255)

                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    >  1:5501348/1‑93 (MQ=255)

                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   >  2:5226324/1‑93 (MQ=255)

                       gCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTgg                                                                  >  1:2894036/1‑93 (MQ=255)

                        cacaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 >  2:120522/1‑93 (MQ=255)

                        cacaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 <  1:1272324/93‑1 (MQ=255)

                         acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                <  1:3763140/93‑1 (MQ=255)

                         acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                >  2:3745087/1‑93 (MQ=255)

                         acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                <  2:5484133/93‑1 (MQ=255)

                          caTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTAc                                                               >  2:848534/1‑93 (MQ=255)

                          caTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTAc                                                               <  1:1241837/93‑1 (MQ=255)

                            tCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAg                                                             >  1:5401479/1‑93 (MQ=255)

                             cTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGc                                                            <  2:6229687/93‑1 (MQ=255)

                              ttCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCt                                                           >  2:6030043/1‑93 (MQ=255)

                                 aaTTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAt                                                        <  2:269136/93‑1 (MQ=255)

                                   tttCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAttt                                                      >  2:5881939/1‑93 (MQ=255)

                                     tCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTc                                                    <  1:4173882/93‑1 (MQ=255)

                                     tCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTc                                                    <  2:2257734/93‑1 (MQ=255)

                                      cAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCt                                                   <  1:4107601/93‑1 (MQ=255)

                                      cAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCt                                                   <  2:254307/93‑1 (MQ=255)

                                       aGTTCTAATCACATTCATTCATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTTTTTATTATTGTTACAGCTAATTTTCtt                                                  >  2:6130587/1‑93 (MQ=255)

                                        gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 >  2:3115497/1‑93 (MQ=255)

                                          tCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACt                                               >  2:4180807/1‑93 (MQ=255)

                                           cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              >  1:2518402/1‑93 (MQ=255)

                                           cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              >  2:44708/1‑93 (MQ=255)

                                             aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            >  2:3845073/1‑93 (MQ=255)

                                               tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  1:2033423/1‑93 (MQ=255)

                                                 acaTTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCaa                                        <  2:4692849/93‑1 (MQ=255)

                                                   attcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACa                                      <  1:5288037/93‑1 (MQ=255)

                                                     tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    <  2:3697107/93‑1 (MQ=255)

                                                     tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    >  2:3074947/1‑93 (MQ=255)

                                                     tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    <  1:2712516/93‑1 (MQ=255)

                                                     tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    <  2:1116188/93‑1 (MQ=255)

                                                     tcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATc                                    <  2:5378635/93‑1 (MQ=255)

                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:4155909/1‑93 (MQ=255)

                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  2:2303612/1‑93 (MQ=255)

                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  <  1:1637797/93‑1 (MQ=255)

                                                        ttAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAAt                                 <  1:4437852/93‑1 (MQ=255)

                                                         tAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATa                                >  1:5900825/1‑93 (MQ=255)

                                                          aaTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaa                               >  2:154768/1‑93 (MQ=255)

                                                           aTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaa                              >  2:1377852/1‑93 (MQ=255)

                                                                aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         <  1:18333/93‑1 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  2:458403/93‑1 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  1:5350979/93‑1 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        >  1:5581743/1‑93 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  2:1756577/93‑1 (MQ=255)

                                                                  tCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTa                       <  1:4989589/93‑1 (MQ=255)

                                                                  tCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCGTACTTGCTCAACATCAATAAAACCTTTa                       >  1:6061054/1‑93 (MQ=255)

                                                                  tCCATCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTa                       <  1:6302180/93‑1 (MQ=255)

                                                                   ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      >  1:3882511/1‑93 (MQ=255)

                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:2594102/1‑93 (MQ=255)

                                                                     ttCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGa                    >  1:2909513/1‑93 (MQ=255)

                                                                      tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   <  1:4414718/93‑1 (MQ=255)

                                                                      tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   <  1:4768582/93‑1 (MQ=255)

                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  2:3330649/93‑1 (MQ=255)

                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:595506/93‑1 (MQ=255)

                                                                        gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 >  1:5636572/1‑93 (MQ=255)

                                                                         tCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATg                >  1:6114477/1‑93 (MQ=255)

                                                                           aTTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCa              >  2:3222124/1‑93 (MQ=255)

                                                                            ttttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCaa             >  1:2605832/1‑93 (MQ=255)

                                                                            ttttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCaa             >  2:457319/1‑93 (MQ=255)

                                                                            ttttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCaa             >  2:5556660/1‑93 (MQ=255)

                                                                             tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            <  2:5243177/93‑1 (MQ=255)

                                                                              ttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtc           <  1:654078/93‑1 (MQ=255)

                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         >  1:2328247/1‑93 (MQ=255)

                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         <  2:2834909/93‑1 (MQ=255)

                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGACAtctc         >  1:2734821/1‑93 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:577878/93‑1 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:791763/93‑1 (MQ=255)

                                                                                    aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     >  1:3596594/1‑93 (MQ=255)

                                                                                    aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     >  1:3182064/1‑93 (MQ=255)

                                                                                     gATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    <  1:3104295/93‑1 (MQ=255)

AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAGG  >  NC_000913/2108101‑2108287

ACCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACC                                                                                               <  SRR4302533.2367786/94‑1 (MQ=60)

ACCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACC                                                                                               <  SRR4302533.1397152/94‑1 (MQ=60)

 CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCTATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTGATTTTATAAAGATACCG                                                                                              >  SRR4302533.4977144/1‑94 (MQ=60)

 CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCG                                                                                              <  SRR4302533.4261099/94‑1 (MQ=60)

 CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCG                                                                                              <  SRR4302533.3045688/94‑1 (MQ=60)

 CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCG                                                                                              <  SRR4302533.674674/94‑1 (MQ=60)

   AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCC                                                                                            >  SRR4302533.468567/1‑94 (MQ=60)

    AATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCC                                                                                           >  SRR4302533.2053701/1‑94 (MQ=60)

      TACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCAT                                                                                         <  SRR4302533.4338/94‑1 (MQ=60)

        CTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAA                                                                                       >  SRR4302533.222084/1‑94 (MQ=60)

        CTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAA                                                                                       >  SRR4302533.2304789/1‑94 (MQ=60)

         TCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAAT                                                                                      >  SRR4302533.374055/1‑94 (MQ=60)

          CTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATT                                                                                     <  SRR4302533.3167059/94‑1 (MQ=60)

          CTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATT                                                                                     >  SRR4302533.432035/1‑94 (MQ=60)

          CTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATT                                                                                     <  SRR4302533.4442849/94‑1 (MQ=60)

            TGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTAT                                                                                   <  SRR4302533.5943611/94‑1 (MQ=60)

               CTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCT                                                                                >  SRR4302533.1057936/1‑94 (MQ=60)

               CTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCT                                                                                >  SRR4302533.4275565/1‑94 (MQ=60)

               CTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCT                                                                                >  SRR4302533.1006160/1‑94 (MQ=60)

                TCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTT                                                                               <  SRR4302533.5162036/94‑1 (MQ=60)

                 CCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTT                                                                              >  SRR4302533.4450821/1‑94 (MQ=60)

                   AGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTAT                                                                            >  SRR4302533.2918052/1‑94 (MQ=60)

                    GAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATT                                                                           <  SRR4302533.2944116/94‑1 (MQ=60)

                    GAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATT                                                                           >  SRR4302533.1949572/1‑94 (MQ=60)

                      ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                         <  SRR4302533.2536513/94‑1 (MQ=60)

                      ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                         <  SRR4302533.1723018/94‑1 (MQ=60)

                      ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                         <  SRR4302533.5429253/94‑1 (MQ=60)

                      ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                         <  SRR4302533.5902388/94‑1 (MQ=60)

                       TTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGT                                                                        >  SRR4302533.790034/1‑94 (MQ=60)

                       TTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGT                                                                        <  SRR4302533.2197554/94‑1 (MQ=60)

                       TTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGT                                                                        >  SRR4302533.5534534/1‑94 (MQ=60)

                         AGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGG                                                                      >  SRR4302533.2911818/1‑94 (MQ=60)

                          GCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGT                                                                     >  SRR4302533.5334472/1‑94 (MQ=60)

                          GCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGT                                                                     <  SRR4302533.5640992/94‑1 (MQ=60)

                           CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                    >  SRR4302533.4912791/1‑94 (MQ=60)

                           CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                    <  SRR4302533.1279934/94‑1 (MQ=60)

                            ACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTAC                                                                   <  SRR4302533.3786207/94‑1 (MQ=60)

                             CATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACA                                                                  <  SRR4302533.3128586/94‑1 (MQ=60)

                             CATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACA                                                                  <  SRR4302533.760572/94‑1 (MQ=60)

                             CATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACA                                                                  <  SRR4302533.1249257/94‑1 (MQ=60)

                              ATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAG                                                                 >  SRR4302533.5433938/1‑94 (MQ=60)

                              ATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAG                                                                 >  SRR4302533.2872662/1‑94 (MQ=60)

                               TCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGC                                                                >  SRR4302533.2408363/1‑94 (MQ=60)

                                  TCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAA                                                             >  SRR4302533.404855/1‑94 (MQ=60)

                                       TTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTC                                                        >  SRR4302533.1967795/1‑94 (MQ=60)

                                       TTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTC                                                        >  SRR4302533.5303211/1‑94 (MQ=60)

                                        TCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCT                                                       <  SRR4302533.4199176/94‑1 (MQ=60)

                                         CAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTT                                                      <  SRR4302533.4132575/94‑1 (MQ=60)

                                          AGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTA                                                     >  SRR4302533.1899212/1‑94 (MQ=60)

                                            TTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGTCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACT                                                   <  SRR4302533.949890/94‑1 (MQ=60)

                                            TTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACT                                                   >  SRR4302533.6186522/1‑94 (MQ=60)

                                             TCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTT                                                  >  SRR4302533.2533917/1‑94 (MQ=60)

                                              CTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTG                                                 <  SRR4302533.4234672/94‑1 (MQ=60)

                                                AATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCT                                               >  SRR4302533.6143413/1‑94 (MQ=60)

                                                 ATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTC                                              >  SRR4302533.2045967/1‑94 (MQ=60)

                                                  TCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCA                                             >  SRR4302533.637179/1‑94 (MQ=60)

                                                   CACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAA                                            >  SRR4302533.2987344/1‑94 (MQ=60)

                                                      ATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAT                                         <  SRR4302533.5319706/94‑1 (MQ=60)

                                                        TCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCA                                       <  SRR4302533.2729171/94‑1 (MQ=60)

                                                         CATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAA                                      >  SRR4302533.4181089/1‑94 (MQ=60)

                                                          ATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAAT                                     <  SRR4302533.1647713/94‑1 (MQ=60)

                                                          ATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAAT                                     <  SRR4302533.3859315/94‑1 (MQ=60)

                                                          ATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAAT                                     <  SRR4302533.5985439/94‑1 (MQ=60)

                                                           TTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATA                                    >  SRR4302533.3774185/1‑94 (MQ=60)

                                                           TTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATA                                    >  SRR4302533.5936460/1‑94 (MQ=60)

                                                           TTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATA                                    <  SRR4302533.4464680/94‑1 (MQ=60)

                                                                TTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACC                               <  SRR4302533.3590043/94‑1 (MQ=60)

                                                                TTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACC                               >  SRR4302533.4190210/1‑94 (MQ=60)

                                                                TTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACC                               <  SRR4302533.5450969/94‑1 (MQ=60)

                                                                  GAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTT                             <  SRR4302533.4287033/94‑1 (MQ=60)

                                                                   AATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTT                            <  SRR4302533.18458/94‑1 (MQ=60)

                                                                   AATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTT                            >  SRR4302533.5615470/1‑94 (MQ=60)

                                                                   AATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTT                            <  SRR4302533.1352781/94‑1 (MQ=60)

                                                                    ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTA                           >  SRR4302533.416540/1‑94 (MQ=60)

                                                                    ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTA                           <  SRR4302533.5383088/94‑1 (MQ=60)

                                                                    ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCGTACTTGCTCAACATCAATAAAACCTTTA                           >  SRR4302533.6097744/1‑94 (MQ=60)

                                                                     TCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAC                          >  SRR4302533.3906202/1‑94 (MQ=60)

                                                                     TCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAC                          <  SRR4302533.5019839/94‑1 (MQ=60)

                                                                     TCCATCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAC                          <  SRR4302533.6339828/94‑1 (MQ=60)

                                                                      CCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACG                         >  SRR4302533.3186098/1‑94 (MQ=60)

                                                                       CTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGA                        >  SRR4302533.2927376/1‑94 (MQ=60)

                                                                        TTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAA                       <  SRR4302533.6063747/94‑1 (MQ=60)

                                                                         TCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAA                      <  SRR4302533.4441375/94‑1 (MQ=60)

                                                                         TCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAA                      <  SRR4302533.4797486/94‑1 (MQ=60)

                                                                          CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                     >  SRR4302533.5566259/1‑94 (MQ=60)

                                                                          CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                     >  SRR4302533.5670655/1‑94 (MQ=60)

                                                                          CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                     <  SRR4302533.4329555/94‑1 (MQ=60)

                                                                          CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                     <  SRR4302533.599018/94‑1 (MQ=60)

                                                                           GTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATG                    >  SRR4302533.6151372/1‑94 (MQ=60)

                                                                              ATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAA                 >  SRR4302533.2621837/1‑94 (MQ=60)

                                                                               TTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAT                <  SRR4302533.223046/94‑1 (MQ=60)

                                                                                 TTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCT              <  SRR4302533.657937/94‑1 (MQ=60)

                                                                                  TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC             >  SRR4302533.2342660/1‑94 (MQ=60)

                                                                                  TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC             <  SRR4302533.4495412/94‑1 (MQ=60)

                                                                                  TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGACATCTC             >  SRR4302533.2751597/1‑94 (MQ=60)

                                                                                   ATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCT            >  SRR4302533.1266391/1‑94 (MQ=60)

                                                                                   ATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCT            >  SRR4302533.5937567/1‑94 (MQ=60)

                                                                                   ATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCT            <  SRR4302533.2338799/94‑1 (MQ=60)

                                                                                     AAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTC          <  SRR4302533.367218/94‑1 (MQ=60)

                                                                                      AAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCA         <  SRR4302533.4323056/94‑1 (MQ=60)

                                                                                      AAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCA         >  SRR4302533.3201641/1‑94 (MQ=60)

                                                                                      AAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCA         >  SRR4302533.3618725/1‑94 (MQ=60)

                                                                                       AGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAG        <  SRR4302533.2941866/94‑1 (MQ=60)

                                                                                       AGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAG        <  SRR4302533.2718959/94‑1 (MQ=60)

                                                                                        GATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGG       >  SRR4302533.4457834/1‑94 (MQ=60)

                                                                                        GATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGG       <  SRR4302533.3123378/94‑1 (MQ=60)

                                                                                         ATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA      >  SRR4302533.2209697/1‑94 (MQ=60)

                                                                                         ATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA      >  SRR4302533.2740991/1‑94 (MQ=60)

                                                                                         ATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA      >  SRR4302533.5054021/1‑94 (MQ=60)

                                                                                             CGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAGG  <  SRR4302533.5597026/94‑1 (MQ=60)

ACCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAGG  >  NC_000913/2108101‑2108287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`