Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,654 T→C A232A (GCA→GCG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,6540TC100.0% 800.7 / NA 248A232A (GCA→GCG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (119/129);  total (119/129)

Only 100 of 253 total aligned reads displayed.

GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG  >  NC_000913/2111564‑2111743
                                                                                          |                                                                                         
gTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                                                                                         >  1:2754164/1‑93 (MQ=255)
gTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                                                                                         >  1:190773/1‑93 (MQ=255)
  cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                       <  1:3278828/93‑1 (MQ=255)
    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                     <  1:4905052/93‑1 (MQ=255)
    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                     >  2:2269719/1‑93 (MQ=255)
     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                    <  1:5417268/93‑1 (MQ=255)
      aCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAgg                                                                                   >  2:5074817/1‑93 (MQ=255)
       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcccagc                                                                                  >  1:1113120/1‑89 (MQ=255)
         gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg                                                                                <  2:2453727/93‑1 (MQ=255)
         gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg                                                                                >  2:4315155/1‑93 (MQ=255)
          tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                               >  2:2237694/1‑93 (MQ=255)
           cGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAg                                                                              <  1:6099462/93‑1 (MQ=255)
           cGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAg                                                                              <  2:5884480/93‑1 (MQ=255)
            ctcGGTCATCAGCGTTGCATCAGCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt                                                                             <  2:4064959/91‑1 (MQ=255)
             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                            <  2:3280298/93‑1 (MQ=255)
              cGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAc                                                                           <  1:2268457/93‑1 (MQ=255)
               ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg                                                                          >  2:3926695/1‑93 (MQ=255)
               ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg                                                                          >  1:4209791/1‑93 (MQ=255)
                gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                         >  2:2592757/1‑93 (MQ=255)
                   ctcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                      <  2:4346058/92‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                      >  1:296119/1‑93 (MQ=255)
                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                     >  2:933333/1‑93 (MQ=255)
                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                     >  2:1491750/1‑93 (MQ=255)
                     caGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAg                                                                    <  1:756883/93‑1 (MQ=255)
                      aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt                                                                   >  1:6277520/1‑93 (MQ=255)
                       gcgttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGtt                                                                  >  1:1015450/4‑93 (MQ=255)
                       gcgttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGtt                                                                  <  1:1386301/90‑1 (MQ=255)
                         gttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTcc                                                                >  2:4950/2‑93 (MQ=255)
                          ttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGGGTACGGTCCAGTTCCg                                                               >  2:16153/1‑93 (MQ=255)
                          ttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCg                                                               >  1:649982/1‑93 (MQ=255)
                           tGCATCAACGGATTGTTTTTGCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCgg                                                              <  2:4206537/93‑1 (MQ=255)
                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCa                                                           >  2:5298647/1‑93 (MQ=255)
                               tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaa                                                          >  1:4095619/1‑93 (MQ=255)
                               tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaa                                                          >  1:970105/1‑93 (MQ=255)
                                cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                         >  2:5547615/1‑93 (MQ=255)
                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                        <  2:2823122/93‑1 (MQ=255)
                                  aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCCCGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAata                                                       >  2:3729473/1‑93 (MQ=255)
                                   cGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAatat                                                      <  2:1261112/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                     <  2:1788374/93‑1 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGCGTACGTTCCAGTTCCGGCCAAATATCg                                                    >  1:1838563/1‑93 (MQ=255)
                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                    >  2:4490874/1‑93 (MQ=255)
                                       ttGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGc                                                  <  1:5879682/93‑1 (MQ=255)
                                       ttGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGc                                                  >  2:644563/1‑93 (MQ=255)
                                       ttGTTTTTTCGCCAGCTCGGCAATAGCAACAGTCCGCTGCATACGTCACCACACACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCgga                                                  >  2:4172636/1‑92 (MQ=255)
                                       tggTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTGACAGTTCCGGCCAAATATCGGc                                                  >  2:865226/3‑93 (MQ=255)
                                          ttttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGa                                               >  1:2091300/1‑93 (MQ=255)
                                           tttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaa                                              >  2:5258528/1‑93 (MQ=255)
                                           tttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaa                                              <  1:1938070/93‑1 (MQ=255)
                                             tttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAg                                            <  2:3616569/93‑1 (MQ=255)
                                               tCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGca                                          <  1:469226/93‑1 (MQ=255)
                                                cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcac                                         >  1:1623315/1‑93 (MQ=255)
                                                cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcac                                         <  2:5377240/93‑1 (MQ=255)
                                                 gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                        <  2:6207315/93‑1 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                       <  1:2084451/93‑1 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                       >  1:5839330/1‑93 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      <  2:2135658/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      <  1:2422013/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      <  2:5757921/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      >  2:5708070/1‑93 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      >  1:1584368/1‑93 (MQ=255)
                                                    aGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAg                                     <  2:770902/93‑1 (MQ=255)
                                                    aGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAg                                     <  2:4552835/93‑1 (MQ=255)
                                                    aGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGAACATAg                                     >  1:4822751/1‑93 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATaac                                    >  2:3356239/1‑91 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                    >  2:4148693/1‑93 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCACATATCGGCAGAAAACACATAGCGAcc                                >  2:1229403/1‑93 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                                >  2:2369280/1‑93 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                                <  2:4815353/93‑1 (MQ=255)
                                                           cAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTa                              >  1:3365964/1‑93 (MQ=255)
                                                           cAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTa                              <  2:73480/93‑1 (MQ=255)
                                                           cAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTa                              <  1:5041893/93‑1 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             <  2:2071214/93‑1 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             <  2:6043705/93‑1 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             >  1:3566454/1‑93 (MQ=255)
                                                              tAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACgg                           >  2:4260136/1‑93 (MQ=255)
                                                               aGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                          <  1:4307934/93‑1 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                         >  2:2871567/1‑93 (MQ=255)
                                                                gCATCAGTCAGCTGAATAAGCGCCCACGCACCAGGCTCAGTAAGTTCCCGTTCCGGCCAAATATCGGCAGAAAGCACCCAGCGCTCTCCGGcc                         >  2:2945129/1‑93 (MQ=255)
                                                                 cATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCa                        >  1:1775436/1‑93 (MQ=255)
                                                                  aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                       >  1:4523083/1‑93 (MQ=255)
                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                      >  1:2670978/1‑93 (MQ=255)
                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                      >  1:809510/1‑93 (MQ=255)
                                                                       tcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGt                  >  1:5355324/1‑93 (MQ=255)
                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                 <  1:2308084/93‑1 (MQ=255)
                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt                >  2:1600242/1‑93 (MQ=255)
                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt                >  2:542503/1‑93 (MQ=255)
                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg               >  2:1910284/1‑93 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa              <  1:6201358/93‑1 (MQ=255)
                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg             >  1:3839819/1‑93 (MQ=255)
                                                                             gAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGt            >  1:868353/1‑93 (MQ=255)
                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc          <  2:5000332/93‑1 (MQ=255)
                                                                                 aCGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAg        <  1:2545701/93‑1 (MQ=255)
                                                                                  cGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGc       <  1:3506506/93‑1 (MQ=255)
                                                                                   gTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCg      <  1:5962840/93‑1 (MQ=255)
                                                                                   gTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCg      <  1:149624/93‑1 (MQ=255)
                                                                                    tCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATGTCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGt     >  1:4428293/1‑93 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    <  2:2673905/93‑1 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt   <  1:392355/93‑1 (MQ=255)
                                                                                       ccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTg  >  2:3787449/1‑93 (MQ=255)
                                                                                       ccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGAGACCTACGGCCATGATGTCTGAGTCCAGCGTCTg  >  1:678566/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG  >  NC_000913/2111564‑2111743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG  >  NC_000913/2111561‑2111745
                                                                                             |                                                                                           
GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                                                                                             >  SRR4302533.2218866/1‑94 (MQ=60)
GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                                                                                             <  SRR4302533.5221249/94‑1 (MQ=60)
  AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                                                                                           >  SRR4302533.191971/1‑94 (MQ=60)
  AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                                                                                           >  SRR4302533.2771055/1‑94 (MQ=60)
     CGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACC                                                                                        <  SRR4302533.3299073/94‑1 (MQ=60)
      GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTATCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                       >  SRR4302533.4467072/1‑94 (MQ=60)
       TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                                                                                      <  SRR4302533.4934773/94‑1 (MQ=60)
       TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                                                                                      <  SRR4302533.4934774/94‑1 (MQ=60)
        AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                                                                                     <  SRR4302533.5449857/94‑1 (MQ=60)
        AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                                                                                     >  SRR4302533.1608394/1‑94 (MQ=60)
        AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                                                                                     <  SRR4302533.3306202/94‑1 (MQ=60)
        AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                                                                                     >  SRR4302533.6200161/1‑94 (MQ=60)
         ACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCcagc                                                                                    >  SRR4302533.1119722/1‑90 (MQ=60)
          CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                                                                                   >  SRR4302533.2010470/1‑94 (MQ=60)
          CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                                                                                   >  SRR4302533.5927582/1‑94 (MQ=60)
           TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCGGCTGAATACGTCCCCACGCACCAGGCTG                                                                                  >  SRR4302533.962820/1‑94 (MQ=60)
             TCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAG                                                                                <  SRR4302533.68771/94‑1 (MQ=60)
              CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGT                                                                               <  SRR4302533.6136298/94‑1 (MQ=60)
                CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                             <  SRR4302533.5641141/94‑1 (MQ=60)
                 CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG                                                                            <  SRR4302533.2282494/94‑1 (MQ=60)
                 CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG                                                                            >  SRR4302533.4235257/1‑94 (MQ=60)
                 CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG                                                                            >  SRR4302533.5819463/1‑94 (MQ=60)
                  GGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGT                                                                           >  SRR4302533.832545/1‑94 (MQ=60)
                    TCATCAGCGTTGCATCAACGGATTGTTCTTCCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTC                                                                         <  SRR4302533.2344200/94‑1 (MQ=60)
                     CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC                                                                        >  SRR4302533.297930/1‑94 (MQ=60)
                     CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAAGCCCCAGGCTGAGTCCGTTCC                                                                        >  SRR4302533.1750148/1‑94 (MQ=60)
                     CATCAGCGTTGCATCAACGGATTGTTTTTGCGCCAGCCCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCTCCAGGCTGAGTACGTTCC                                                                        <  SRR4302533.6243388/94‑1 (MQ=60)
                      ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                       <  SRR4302533.4115920/94‑1 (MQ=60)
                      ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                       >  SRR4302533.1866449/1‑94 (MQ=60)
                        CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT                                                                     <  SRR4302533.1965515/94‑1 (MQ=60)
                        CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT                                                                     >  SRR4302533.6315001/1‑94 (MQ=60)
                        CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT                                                                     <  SRR4302533.761337/94‑1 (MQ=60)
                         AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT                                                                    <  SRR4302533.4547722/94‑1 (MQ=60)
                         AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT                                                                    >  SRR4302533.1021415/1‑94 (MQ=60)
                          GCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTC                                                                   <  SRR4302533.1394643/94‑1 (MQ=60)
                           CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC                                                                  >  SRR4302533.3971977/1‑94 (MQ=60)
                            GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG                                                                 <  SRR4302533.2409916/94‑1 (MQ=60)
                            GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG                                                                 <  SRR4302533.642852/94‑1 (MQ=60)
                            GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG                                                                 >  SRR4302533.653818/1‑94 (MQ=60)
                             TTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                <  SRR4302533.4844824/94‑1 (MQ=60)
                             TTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                <  SRR4302533.1185239/94‑1 (MQ=60)
                              TGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGC                                                               >  SRR4302533.4235922/1‑94 (MQ=60)
                                CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCA                                                             >  SRR4302533.2994396/1‑94 (MQ=60)
                                CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCA                                                             >  SRR4302533.6323415/1‑94 (MQ=60)
                                 ATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAA                                                            >  SRR4302533.975795/1‑94 (MQ=60)
                                 ATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAA                                                            >  SRR4302533.4120553/1‑94 (MQ=60)
                                     ACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATAT                                                        <  SRR4302533.5951420/94‑1 (MQ=60)
                                      CGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATC                                                       <  SRR4302533.671191/94‑1 (MQ=60)
                                      CGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATC                                                       >  SRR4302533.3265548/1‑94 (MQ=60)
                                      CGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATC                                                       >  SRR4302533.3932415/1‑94 (MQ=60)
                                       GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGCGTACGTTCCAGTTCCGGCCAAATATCG                                                      >  SRR4302533.1849774/1‑94 (MQ=60)
                                        GATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGG                                                     <  SRR4302533.1641917/94‑1 (MQ=60)
                                          TTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCA                                                   <  SRR4302533.5915217/94‑1 (MQ=60)
                                            GTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGA                                                 >  SRR4302533.2104181/1‑94 (MQ=60)
                                              TTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAA                                               >  SRR4302533.3643710/1‑94 (MQ=60)
                                              TTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAA                                               >  SRR4302533.2293582/1‑94 (MQ=60)
                                              TTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAA                                               <  SRR4302533.1949885/94‑1 (MQ=60)
                                              TTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAA                                               >  SRR4302533.2234140/1‑94 (MQ=60)
                                               TTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAG                                              <  SRR4302533.611404/94‑1 (MQ=60)
                                                 TTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCA                                            <  SRR4302533.1553124/94‑1 (MQ=60)
                                                  TCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCAC                                           >  SRR4302533.1633147/1‑94 (MQ=60)
                                                  TCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCAC                                           >  SRR4302533.1858795/1‑94 (MQ=60)
                                                  TCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCAC                                           <  SRR4302533.472029/94‑1 (MQ=60)
                                                   CGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACA                                          <  SRR4302533.3530806/94‑1 (MQ=60)
                                                    GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                         <  SRR4302533.4793633/94‑1 (MQ=60)
                                                    GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                         >  SRR4302533.5874635/1‑94 (MQ=60)
                                                    GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                         <  SRR4302533.2749424/94‑1 (MQ=60)
                                                     CCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATA                                        >  SRR4302533.1593914/1‑94 (MQ=60)
                                                     CCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATA                                        <  SRR4302533.2097284/94‑1 (MQ=60)
                                                      CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAG                                       <  SRR4302533.4216544/94‑1 (MQ=60)
                                                      CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAG                                       <  SRR4302533.858705/94‑1 (MQ=60)
                                                      CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAG                                       <  SRR4302533.2436965/94‑1 (MQ=60)
                                                      CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGAACATAG                                       >  SRR4302533.4851971/1‑94 (MQ=60)
                                                        GCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCG                                     <  SRR4302533.8161/94‑1 (MQ=60)
                                                         CTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGA                                    >  SRR4302533.4602856/1‑94 (MQ=60)
                                                           CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                  <  SRR4302533.2947406/94‑1 (MQ=60)
                                                           CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                  >  SRR4302533.141097/1‑94 (MQ=60)
                                                           CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                  >  SRR4302533.5322332/1‑94 (MQ=60)
                                                           CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                  <  SRR4302533.340225/94‑1 (MQ=60)
                                                           CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                  <  SRR4302533.2621532/94‑1 (MQ=60)
                                                             GCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTA                                >  SRR4302533.3386755/1‑94 (MQ=60)
                                                             GCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTA                                <  SRR4302533.5345937/94‑1 (MQ=60)
                                                              CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAC                               >  SRR4302533.5038572/1‑94 (MQ=60)
                                                              CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAC                               <  SRR4302533.5072407/94‑1 (MQ=60)
                                                              CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAC                               >  SRR4302533.3588432/1‑94 (MQ=60)
                                                               AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                              <  SRR4302533.210248/94‑1 (MQ=60)
                                                               AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                              <  SRR4302533.1026323/94‑1 (MQ=60)
                                                               AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                              <  SRR4302533.5903120/94‑1 (MQ=60)
                                                                ATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGG                             <  SRR4302533.2437202/94‑1 (MQ=60)
                                                                 TAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGC                            <  SRR4302533.367932/94‑1 (MQ=60)
                                                                 TAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGC                            <  SRR4302533.2624956/94‑1 (MQ=60)
                                                                  AGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCC                           <  SRR4302533.4333862/94‑1 (MQ=60)
                                                                  AGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCC                           >  SRR4302533.1932588/1‑94 (MQ=60)
                                                                   GCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCA                          >  SRR4302533.4424683/1‑94 (MQ=60)
                                                                   GCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCA                          >  SRR4302533.1786247/1‑94 (MQ=60)
                                                                    CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                         <  SRR4302533.997620/94‑1 (MQ=60)
                                                                    CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                         >  SRR4302533.4550463/1‑94 (MQ=60)
                                                                    CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                         <  SRR4302533.1530613/94‑1 (MQ=60)
                                                                     ATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATG                        >  SRR4302533.814263/1‑94 (MQ=60)
                                                                     ATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATG                        >  SRR4302533.714463/1‑94 (MQ=60)
                                                                     ATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATG                        >  SRR4302533.2687360/1‑94 (MQ=60)
                                                                       CAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGAT                      <  SRR4302533.5411100/94‑1 (MQ=60)
                                                                       CAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGAT                      <  SRR4302533.1345663/94‑1 (MQ=60)
                                                                       CAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGAT                      <  SRR4302533.1846513/94‑1 (MQ=60)
                                                                         GTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGT                    >  SRR4302533.5387470/1‑94 (MQ=60)
                                                                           CAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCT                  <  SRR4302533.2124301/94‑1 (MQ=60)
                                                                           CAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCT                  <  SRR4302533.2322381/94‑1 (MQ=60)
                                                                            AGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTA                 >  SRR4302533.3905065/1‑94 (MQ=60)
                                                                              CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG               <  SRR4302533.6238552/94‑1 (MQ=60)
                                                                              CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG               >  SRR4302533.3863277/1‑94 (MQ=60)
                                                                               TGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGT              >  SRR4302533.873442/1‑94 (MQ=60)
                                                                               TGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGT              >  SRR4302533.2691186/1‑94 (MQ=60)
                                                                                GAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTC             >  SRR4302533.6216217/1‑94 (MQ=60)
                                                                                    ACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGC         <  SRR4302533.2561364/94‑1 (MQ=60)
                                                                                     CGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCG        <  SRR4302533.3528126/94‑1 (MQ=60)
                                                                                     CGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCG        <  SRR4302533.4965245/94‑1 (MQ=60)
                                                                                      GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATGTCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGT       >  SRR4302533.4455062/1‑94 (MQ=60)
                                                                                      GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGT       <  SRR4302533.5998912/94‑1 (MQ=60)
                                                                                      GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGT       <  SRR4302533.150580/94‑1 (MQ=60)
                                                                                        CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT     >  SRR4302533.4269928/1‑94 (MQ=60)
                                                                                         CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG    <  SRR4302533.394733/94‑1 (MQ=60)
                                                                                         CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG    <  SRR4302533.34183/94‑1 (MQ=60)
                                                                                         CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGAGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG    >  SRR4302533.682577/1‑94 (MQ=60)
                                                                                          CCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC   >  SRR4302533.2343061/1‑94 (MQ=60)
                                                                                           CACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG  >  SRR4302533.6235988/1‑94 (MQ=60)
                                                                                           CACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG  <  SRR4302533.6253373/94‑1 (MQ=60)
                                                                                             |                                                                                           
GCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG  >  NC_000913/2111561‑2111745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑