Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,017	Δ1 bp	coding (38/339 nt)	b2107 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,014	0	T	.	100.0%	1006.2 / NA	217	coding (35/339 nt)	b2107	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (109/108); total (109/108)

Only 100 of 235 total aligned reads displayed.

TATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGT  >  NC_000913/2184922‑2185102

tatTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCt                                                                                          >  1:1548704/1‑93 (MQ=255)

tatTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCt                                                                                          >  1:5680187/1‑93 (MQ=255)

  ttGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCttt                                                                                        <  2:1512850/93‑1 (MQ=255)

  ttGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCttt                                                                                        >  2:5775895/1‑93 (MQ=255)

   tGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtttg                                                                                       <  2:3244909/93‑2 (MQ=255)

     tATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtttgct                                                                                     <  2:5764407/93‑4 (MQ=255)

         gCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGtt                                                                                <  1:4437819/93‑1 (MQ=255)

             gAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCa                                                                            >  1:3368702/1‑93 (MQ=255)

              aaaGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAg                                                                           >  2:4306205/1‑93 (MQ=255)

              aaaGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAg                                                                           >  2:3587737/1‑93 (MQ=255)

                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         <  1:3947211/93‑1 (MQ=255)

                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         >  1:1944321/1‑93 (MQ=255)

                 gATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgc                                                                        <  1:4746673/93‑1 (MQ=255)

                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  2:824043/93‑1 (MQ=255)

                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  1:2209219/93‑1 (MQ=255)

                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  1:3031423/93‑1 (MQ=255)

                   tatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccg                                                                      >  1:972088/1‑93 (MQ=255)

                   tatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccg                                                                      >  1:2868363/1‑93 (MQ=255)

                      gCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCt                                                                   <  2:5048142/93‑1 (MQ=255)

                       cGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTg                                                                  <  2:1101783/93‑1 (MQ=255)

                        gACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTgg                                                                 <  1:3001445/93‑1 (MQ=255)

                        gACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTgg                                                                 >  2:616320/1‑93 (MQ=255)

                          cTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCTGTGCCGCCTGGGc                                                               <  2:6191068/93‑1 (MQ=255)

                          cTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGc                                                               <  2:2234651/93‑1 (MQ=255)

                            gATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCg                                                             >  1:1772765/1‑93 (MQ=255)

                             atatTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGc                                                            >  1:4958294/1‑93 (MQ=255)

                             atatTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGc                                                            <  1:1483086/93‑1 (MQ=255)

                               atTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAc                                                          >  1:5127159/1‑93 (MQ=255)

                                ttACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAcc                                                         >  2:5989097/1‑93 (MQ=255)

                                ttACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAcc                                                         <  1:1508356/93‑1 (MQ=255)

                                 tACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTGCCAGTGCCGCCTGGGCCGCACCa                                                        <  1:4229510/93‑1 (MQ=255)

                                 tACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCa                                                        <  1:5064134/93‑1 (MQ=255)

                                 tACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCa                                                        >  1:2693484/1‑93 (MQ=255)

                                  aCAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAg                                                       <  2:5224936/93‑1 (MQ=255)

                                    aGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGcc                                                     >  1:4538292/1‑93 (MQ=255)

                                     gATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCa                                                    >  1:6160475/1‑93 (MQ=255)

                                     gATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCa                                                    >  1:2701434/1‑93 (MQ=255)

                                      aTCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAc                                                   >  2:3093998/1‑93 (MQ=255)

                                       tCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAcc                                                  >  2:3629456/1‑93 (MQ=255)

                                         cccAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACcgc                                                >  2:5863259/1‑93 (MQ=255)

                                         cccAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACcgc                                                >  1:5730213/1‑93 (MQ=255)

                                          ccAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACcgcg                                               >  2:1264041/1‑93 (MQ=255)

                                           cAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCgg                                              >  2:245777/1‑93 (MQ=255)

                                             aaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGt                                            <  2:4501103/93‑1 (MQ=255)

                                             aaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGt                                            >  1:1656896/1‑93 (MQ=255)

                                             aaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGt                                            >  1:863628/1‑93 (MQ=255)

                                               gTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTc                                          >  2:2729259/1‑93 (MQ=255)

                                               gTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTc                                          <  2:729053/93‑1 (MQ=255)

                                                tGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCg                                         >  2:542473/1‑93 (MQ=255)

                                                 ggAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGa                                        <  1:5862797/93‑1 (MQ=255)

                                                 ggAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGa                                        <  1:38041/93‑1 (MQ=255)

                                                  gagaGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAc                                       <  2:5115022/93‑1 (MQ=255)

                                                  gagaGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAc                                       <  1:4860658/93‑1 (MQ=255)

                                                  gagaGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAc                                       >  1:1056638/1‑93 (MQ=255)

                                                   agagTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAcc                                      <  1:3425437/93‑1 (MQ=255)

                                                    gagTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGTGGGTTCGACCa                                     >  2:6062645/1‑93 (MQ=255)

                                                    gagTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCa                                     >  2:300809/1‑93 (MQ=255)

                                                     agTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCaa                                    <  1:1150707/93‑1 (MQ=255)

                                                      gTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAAt                                   >  1:2270815/1‑93 (MQ=255)

                                                       tttATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATa                                  <  1:4312721/93‑1 (MQ=255)

                                                          aTGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCt                               <  1:4845304/93‑1 (MQ=255)

                                                          aTGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCt                               <  2:4390953/93‑1 (MQ=255)

                                                           tGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTc                              >  1:1820375/1‑93 (MQ=255)

                                                            gACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCg                             >  1:6090605/1‑93 (MQ=255)

                                                              ccATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCggg                           <  2:3846506/93‑1 (MQ=255)

                                                               cATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGa                          >  2:1391014/1‑93 (MQ=255)

                                                                aTTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGaa                         >  2:1771084/1‑93 (MQ=255)

                                                                aTTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGaa                         >  1:3673264/1‑93 (MQ=255)

                                                                aTTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGaa                         >  2:2410073/1‑93 (MQ=255)

                                                                 ttAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAt                        <  1:666589/93‑1 (MQ=255)

                                                                 ttAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAt                        >  2:2729436/1‑93 (MQ=255)

                                                                   aaaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAttt                      >  2:6284568/1‑93 (MQ=255)

                                                                   aaaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAttt                      <  2:5544335/93‑1 (MQ=255)

                                                                   aaaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGGTCGACCAATACCTCGGGAAttt                      >  2:3394884/1‑93 (MQ=255)

                                                                    aaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTc                     <  1:4344122/93‑1 (MQ=255)

                                                                    aaaaTAAGAGGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTc                     <  2:1413596/93‑1 (MQ=255)

                                                                     aaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCt                    >  1:3693913/1‑93 (MQ=255)

                                                                      aaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTa                   <  2:4194287/93‑1 (MQ=255)

                                                                       aTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTaa                  <  2:2950122/93‑1 (MQ=255)

                                                                       aTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTaa                  <  1:4118643/93‑1 (MQ=255)

                                                                        tAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAg                 >  2:4607996/1‑93 (MQ=255)

                                                                         aaGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGt                >  1:6027107/1‑93 (MQ=255)

                                                                           gATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAt              >  1:6210485/1‑93 (MQ=255)

                                                                           gATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAt              >  1:2417098/1‑93 (MQ=255)

                                                                            aTGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATg             <  2:3664399/93‑1 (MQ=255)

                                                                             tGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGa            >  2:2595575/1‑93 (MQ=255)

                                                                             tGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGa            <  1:2004080/93‑1 (MQ=255)

                                                                               ttGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGt          <  1:5420260/93‑1 (MQ=255)

                                                                                tGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGtt         <  2:3509666/93‑1 (MQ=255)

                                                                                 gCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTa        >  1:2047343/1‑93 (MQ=255)

                                                                                  cTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTaa       <  1:1290316/93‑1 (MQ=255)

                                                                                   tGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAag      <  2:4134355/93‑1 (MQ=255)

                                                                                   tGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAag      >  1:6227965/1‑93 (MQ=255)

                                                                                   tGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAag      >  2:502046/1‑93 (MQ=255)

                                                                                    gggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagc     >  1:5010211/1‑92 (MQ=255)

                                                                                     ggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagca    >  1:551381/1‑91 (MQ=255)

                                                                                     ggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagca    <  2:5163280/93‑3 (MQ=255)

                                                                                     ggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagca    >  2:4552733/1‑91 (MQ=255)

                                                                                      gTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagcag   <  1:4304568/93‑1 (MQ=255)

                                                                                       tGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagcagt  >  1:3217225/1‑93 (MQ=255)

TATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGT  >  NC_000913/2184922‑2185102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGTTTCATTGC  >  NC_000913/2184924‑2185110

TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTG                                                                                               >  SRR4302533.1250519/1‑94 (MQ=60)

TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTG                                                                                               <  SRR4302533.512061/94‑1 (MQ=60)

   TATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTG                                                                                           >  SRR4302533.4425151/1‑94 (MQ=60)

    ATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGG                                                                                          <  SRR4302533.1501432/94‑1 (MQ=60)

       GCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTA                                                                                       <  SRR4302533.4464647/94‑1 (MQ=60)

        CAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTAC                                                                                      >  SRR4302533.3191289/1‑94 (MQ=60)

         AGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACC                                                                                     >  SRR4302533.1966635/1‑94 (MQ=60)

         AGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACC                                                                                     >  SRR4302533.4882975/1‑94 (MQ=60)

          GGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCA                                                                                    >  SRR4302533.889454/1‑94 (MQ=60)

          GGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCA                                                                                    >  SRR4302533.3389507/1‑94 (MQ=60)

           GAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAG                                                                                   <  SRR4302533.5134215/94‑1 (MQ=60)

           GAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAG                                                                                   <  SRR4302533.3228608/94‑1 (MQ=60)

             AAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTG                                                                                 >  SRR4302533.1956180/1‑94 (MQ=60)

              AGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGC                                                                                <  SRR4302533.3971282/94‑1 (MQ=60)

               GATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCC                                                                               <  SRR4302533.4775451/94‑1 (MQ=60)

                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              <  SRR4302533.3049973/94‑1 (MQ=60)

                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              >  SRR4302533.2886010/1‑94 (MQ=60)

                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              <  SRR4302533.2222844/94‑1 (MQ=60)

                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              >  SRR4302533.977797/1‑94 (MQ=60)

                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            <  SRR4302533.4510934/94‑1 (MQ=60)

                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACAATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            >  SRR4302533.829179/1‑94 (MQ=60)

                    GCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTG                                                                          >  SRR4302533.2216200/1‑94 (MQ=60)

                    GCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTG                                                                          <  SRR4302533.1846111/94‑1 (MQ=60)

                     CGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGG                                                                         >  SRR4302533.4158140/1‑94 (MQ=60)

                      GACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGG                                                                        <  SRR4302533.3019800/94‑1 (MQ=60)

                       ACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGC                                                                       >  SRR4302533.397830/1‑94 (MQ=60)

                       ACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGC                                                                       >  SRR4302533.3487642/1‑94 (MQ=60)

                         TGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCG                                                                     >  SRR4302533.1783562/1‑94 (MQ=60)

                          GATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGC                                                                    >  SRR4302533.4988330/1‑94 (MQ=60)

                          GATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCAC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGC                                                                    >  SRR4302533.1482521/1‑94 (MQ=60)

                           ATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCA                                                                   <  SRR4302533.1492040/94‑1 (MQ=60)

                            TATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAC                                                                  >  SRR4302533.5158133/1‑94 (MQ=60)

                             ATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACC                                                                 >  SRR4302533.1149561/1‑94 (MQ=60)

                              TTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCA                                                                <  SRR4302533.1517448/94‑1 (MQ=60)

                              TTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCA                                                                <  SRR4302533.5562596/94‑1 (MQ=60)

                              TTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCA                                                                >  SRR4302533.2709986/1‑94 (MQ=60)

                               TACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTGCCAGTGCCGCCTGGGCCGCACCAG                                                               <  SRR4302533.4255079/94‑1 (MQ=60)

                               TACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAG                                                               <  SRR4302533.6003791/94‑1 (MQ=60)

                               TACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAG                                                               <  SRR4302533.5094727/94‑1 (MQ=60)

                                ACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGC                                                              >  SRR4302533.4985212/1‑94 (MQ=60)

                                 CAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCC                                                             >  SRR4302533.4565769/1‑94 (MQ=60)

                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            >  SRR4302533.2718008/1‑94 (MQ=60)

                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            >  SRR4302533.6197523/1‑94 (MQ=60)

                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            <  SRR4302533.3570649/94‑1 (MQ=60)

                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            <  SRR4302533.501400/94‑1 (MQ=60)

                                    ATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACC                                                          <  SRR4302533.3435239/94‑1 (MQ=60)

                                      CCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGC                                                        >  SRR4302533.5764814/1‑94 (MQ=60)

                                       CCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCG                                                       >  SRR4302533.4346183/1‑94 (MQ=60)

                                        CCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGG                                                      >  SRR4302533.3728231/1‑94 (MQ=60)

                                         CAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGG                                                     <  SRR4302533.3845126/94‑1 (MQ=60)

                                         CAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGG                                                     <  SRR4302533.6128435/94‑1 (MQ=60)

                                          AAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGT                                                    >  SRR4302533.4858633/1‑94 (MQ=60)

                                          AAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGT                                                    >  SRR4302533.1666923/1‑94 (MQ=60)

                                          AAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGT                                                    >  SRR4302533.868688/1‑94 (MQ=60)

                                           AAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTT                                                   <  SRR4302533.2839979/94‑1 (MQ=60)

                                           AAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTCCCGCCTGGGCCGCACCAGCCACCGCGGGTT                                                   <  SRR4302533.5959678/94‑1 (MQ=60)

                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  <  SRR4302533.5020701/94‑1 (MQ=60)

                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  <  SRR4302533.6243771/94‑1 (MQ=60)

                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  <  SRR4302533.2715702/94‑1 (MQ=60)

                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  <  SRR4302533.5048737/94‑1 (MQ=60)

                                              TGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGA                                                >  SRR4302533.288600/1‑94 (MQ=60)

                                               GGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAC                                               <  SRR4302533.5898243/94‑1 (MQ=60)

                                               GGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAC                                               >  SRR4302533.1062851/1‑94 (MQ=60)

                                               GGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAC                                               <  SRR4302533.38291/94‑1 (MQ=60)

                                                GAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACC                                              <  SRR4302533.4890119/94‑1 (MQ=60)

                                                 AGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCA                                             <  SRR4302533.3446596/94‑1 (MQ=60)

                                                   GGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAAT                                           >  SRR4302533.2284866/1‑94 (MQ=60)

                                                   AGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAAT                                           <  SRR4302533.1157599/94‑1 (MQ=60)

                                                     TTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATAC                                         <  SRR4302533.4338685/94‑1 (MQ=60)

                                                      TTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACC                                        >  SRR4302533.1529916/1‑94 (MQ=60)

                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      <  SRR4302533.4874672/94‑1 (MQ=60)

                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      <  SRR4302533.4318834/94‑1 (MQ=60)

                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      >  SRR4302533.1831495/1‑94 (MQ=60)

                                                         TGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCG                                     >  SRR4302533.1611614/1‑94 (MQ=60)

                                                         TGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCG                                     >  SRR4302533.6127418/1‑94 (MQ=60)

                                                           ACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGG                                   <  SRR4302533.6083534/94‑1 (MQ=60)

                                                            CCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGA                                  >  SRR4302533.179007/1‑94 (MQ=60)

                                                             CATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAA                                 >  SRR4302533.3695837/1‑94 (MQ=60)

                                                             CATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAA                                 >  SRR4302533.2241565/1‑94 (MQ=60)

                                                              ATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAT                                <  SRR4302533.3872807/94‑1 (MQ=60)

                                                              ATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAT                                <  SRR4302533.6252068/94‑1 (MQ=60)

                                                               TTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATT                               <  SRR4302533.670527/94‑1 (MQ=60)

                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            >  SRR4302533.3716604/1‑94 (MQ=60)

                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            >  SRR4302533.1711832/1‑94 (MQ=60)

                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            <  SRR4302533.445306/94‑1 (MQ=60)

                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            <  SRR4302533.4370302/94‑1 (MQ=60)

                                                                   AAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTA                           >  SRR4302533.2924871/1‑94 (MQ=60)

                                                                    AATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAA                          <  SRR4302533.5882547/94‑1 (MQ=60)

                                                                    AATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAA                          >  SRR4302533.4607401/1‑94 (MQ=60)

                                                                     ATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAG                         <  SRR4302533.6237186/94‑1 (MQ=60)

                                                                     ATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAG                         <  SRR4302533.4143658/94‑1 (MQ=60)

                                                                      TAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGT                        >  SRR4302533.4785331/1‑94 (MQ=60)

                                                                      TAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGT                        >  SRR4302533.6063630/1‑94 (MQ=60)

                                                                      TAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGT                        <  SRR4302533.3312309/94‑1 (MQ=60)

                                                                        AGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAT                      >  SRR4302533.2432025/1‑94 (MQ=60)

                                                                        AGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAT                      >  SRR4302533.6247731/1‑94 (MQ=60)

                                                                         GATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATG                     >  SRR4302533.3042051/1‑94 (MQ=60)

                                                                          ATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGA                    <  SRR4302533.647977/94‑1 (MQ=60)

                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   <  SRR4302533.2540887/94‑1 (MQ=60)

                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   <  SRR4302533.2016421/94‑1 (MQ=60)

                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   <  SRR4302533.2054927/94‑1 (MQ=60)

                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   >  SRR4302533.1143165/1‑94 (MQ=60)

                                                                             TTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTT                 <  SRR4302533.5452875/94‑1 (MQ=60)

                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                >  SRR4302533.160168/1‑94 (MQ=60)

                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                >  SRR4302533.2059968/1‑94 (MQ=60)

                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                >  SRR4302533.5453136/1‑94 (MQ=60)

                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                >  SRR4302533.2377028/1‑94 (MQ=60)

                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGTGTTAAG              >  SRR4302533.2584201/1‑94 (MQ=60)

                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              >  SRR4302533.5748108/1‑94 (MQ=60)

                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              <  SRR4302533.1298085/94‑1 (MQ=60)

                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              >  SRR4302533.3700202/1‑94 (MQ=60)

                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              >  SRR4302533.6265275/1‑94 (MQ=60)

                                                                                 TGGGTGCGC‑TTTGCTGGTTCCCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGC             <  SRR4302533.1935871/94‑1 (MQ=60)

                                                                                 TGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGC             <  SRR4302533.5579232/94‑1 (MQ=60)

                                                                                 TGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGC             >  SRR4302533.5040565/1‑94 (MQ=60)

                                                                                 TGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGA             >  SRR4302533.4334459/1‑94 (MQ=60)

                                                                                  GGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCA            >  SRR4302533.554649/1‑94 (MQ=60)

                                                                                  GGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCA            <  SRR4302533.144052/94‑1 (MQ=60)

                                                                                   GGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAG           >  SRR4302533.5374250/1‑94 (MQ=60)

                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          >  SRR4302533.755834/1‑94 (MQ=60)

                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          <  SRR4302533.4330468/94‑1 (MQ=60)

                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          >  SRR4302533.4276567/1‑94 (MQ=60)

                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          >  SRR4302533.3237064/1‑94 (MQ=60)

                                                                                     TGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTT         >  SRR4302533.875085/1‑94 (MQ=60)

                                                                                      GCGC‑TTTGCTGGTTCCCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTT        <  SRR4302533.4499172/94‑1 (MQ=60)

                                                                                      GCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTT        <  SRR4302533.1522301/94‑1 (MQ=60)

                                                                                       CGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTC       >  SRR4302533.4130055/1‑94 (MQ=60)

                                                                                       CGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTC       <  SRR4302533.2263872/94‑1 (MQ=60)

                                                                                       CGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTC       <  SRR4302533.5913032/94‑1 (MQ=60)

                                                                                         gcTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCA      >  SRR4302533.1258479/3‑94 (MQ=60)

                                                                                         gcTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCA      >  SRR4302533.971380/3‑94 (MQ=60)

                                                                                          CTTTGCTGGTTCCCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCAT     <  SRR4302533.2165532/94‑1 (MQ=60)

                                                                                          CTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCAT     >  SRR4302533.4100906/1‑94 (MQ=60)

                                                                                          CTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCAT     <  SRR4302533.5124313/94‑1 (MQ=60)

                                                                                           TTTGCTGGTTATCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302533.4268185/94‑1 (MQ=60)

                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302533.4788374/94‑1 (MQ=60)

                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    >  SRR4302533.1858450/1‑94 (MQ=60)

                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    >  SRR4302533.6115329/1‑94 (MQ=60)

                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    >  SRR4302533.2403810/1‑94 (MQ=60)

                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTGC  <  SRR4302533.5834030/94‑1 (MQ=60)

                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTGC  >  SRR4302533.2734883/1‑94 (MQ=60)

                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTGC  >  SRR4302533.4420405/1‑94 (MQ=60)

TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGTTTCATTGC  >  NC_000913/2184924‑2185110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`