Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG100.0% 495.3 / NA 156S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/98);  total (58/98)

Only 100 of 204 total aligned reads displayed.

CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208242‑2208420
                                                                                         |                                                                                         
ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCaa                                                                                        <  2:3029291/93‑1 (MQ=255)
ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCaa                                                                                        <  1:3697379/93‑1 (MQ=255)
 cgcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAag                                                                                       <  1:5517201/93‑1 (MQ=255)
   cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagca                                                                                     <  2:6076938/93‑1 (MQ=255)
     aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCCAATTGTTTCGCAagcagc                                                                                   >  2:3424529/1‑93 (MQ=255)
     aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAagcagc                                                                                   >  1:3110057/1‑93 (MQ=255)
     aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagcagc                                                                                   >  2:1040022/1‑93 (MQ=255)
      aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCAAATTGTTTCGCAAgcagca                                                                                  >  1:4212676/1‑93 (MQ=255)
      aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAgcagca                                                                                  <  2:5588988/93‑1 (MQ=255)
       gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaa                                                                                 <  2:1878457/93‑1 (MQ=255)
        tGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaaa                                                                                <  2:2909128/93‑1 (MQ=255)
           aCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACg                                                                             <  1:4554969/93‑1 (MQ=255)
            cAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCCAATTGTTTTGCAAGGAGCAAAACGc                                                                            >  2:5728722/1‑93 (MQ=255)
             aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  1:6076207/93‑1 (MQ=255)
             aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  2:5871726/93‑1 (MQ=255)
              ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTCGCAAGCAGCAAAACGCAg                                                                          >  2:2687048/1‑93 (MQ=255)
              ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTCGCAAGCAGCAAAAAGCAg                                                                          >  2:1690419/1‑93 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAgg                                                                         >  1:905599/1‑92 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:6227005/93‑1 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:4906416/93‑1 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  2:2546535/93‑1 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:2441544/93‑1 (MQ=255)
               gAAAACTCACCGGCAGCTACGCGTCGGATGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  2:3817093/93‑1 (MQ=255)
                  aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAAAGCAGTGcc                                                                      >  2:4578830/1‑93 (MQ=255)
                  aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGcc                                                                      <  2:3768142/93‑1 (MQ=255)
                  aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATGTTTTGCAAGCAGCAAAACGCAGTGcc                                                                      >  2:5188639/1‑93 (MQ=255)
                     tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  2:279670/93‑1 (MQ=255)
                      ccccGGCAGCTACGCGGCGGCTGTTTAGTTTTCGGGCTGGCGCACTGGCGTGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATg                                                                  <  1:3063544/91‑1 (MQ=255)
                      cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATg                                                                  <  2:524523/93‑1 (MQ=255)
                      cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGGTg                                                                  >  2:3222767/1‑93 (MQ=255)
                         cGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCg                                                               <  2:2108696/93‑1 (MQ=255)
                          ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGGAAATTGTTTTGCAAGCAGCAAAACGCAGTGGCGAAggggg                                                              >  2:393183/1‑90 (MQ=255)
                          ggCAGCGACGCGTCGGCTTTTTAGTTTTCGGGCTGGCGCACTGGGGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              <  2:5264825/93‑1 (MQ=255)
                            cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            <  2:4214327/93‑1 (MQ=255)
                            cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            >  2:1583252/1‑93 (MQ=255)
                            cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            <  2:811471/93‑1 (MQ=255)
                             aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgct                                                           >  2:1210526/1‑93 (MQ=255)
                              gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGGAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGGGAggtg                                                          >  2:144638/1‑93 (MQ=255)
                              gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctg                                                          >  1:574843/1‑93 (MQ=255)
                              gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctg                                                          >  1:5862105/1‑93 (MQ=255)
                                tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGGTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgct                                                        >  2:4019826/1‑93 (MQ=255)
                                 aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGctgctg                                                       >  2:511327/1‑93 (MQ=255)
                                  cgcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGt                                                      <  1:5637285/93‑1 (MQ=255)
                                     gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAATTGTTTCGGAAGCAGCAAAACGCAGATCCGATGGCGGGCTCCTGTTAg                                                   >  1:1168190/1‑93 (MQ=255)
                                        ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTTGCAAGCAGCAAAACGCAGTGGCGATGGCGAGCTGCTGTTAGCTa                                                >  2:669775/1‑93 (MQ=255)
                                         gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               >  2:737389/1‑93 (MQ=255)
                                         gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               >  1:284322/1‑93 (MQ=255)
                                          cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:3110398/1‑93 (MQ=255)
                                          cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:1542276/93‑1 (MQ=255)
                                          cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:2657642/93‑1 (MQ=255)
                                           tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             >  1:3817716/1‑93 (MQ=255)
                                           tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  1:4402400/93‑1 (MQ=255)
                                           tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             >  1:1979252/1‑93 (MQ=255)
                                            gTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGGTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCg                                            >  2:5155840/1‑93 (MQ=255)
                                             tttAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGc                                           >  2:1331461/1‑93 (MQ=255)
                                                aGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAc                                        <  1:1652570/93‑1 (MQ=255)
                                                aGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCCAGCTCCTGTTAGCTACCCGCAAc                                        <  2:5086970/93‑1 (MQ=255)
                                                   tttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     >  1:2230015/1‑93 (MQ=255)
                                                   tttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     <  2:2142930/93‑1 (MQ=255)
                                                    ttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    >  1:6176572/1‑93 (MQ=255)
                                                       aGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTaa                                 <  1:5331313/93‑1 (MQ=255)
                                                        ggCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAag                                <  1:2339014/93‑1 (MQ=255)
                                                         gCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:3471134/93‑1 (MQ=255)
                                                          cTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagca                              <  1:2307146/93‑1 (MQ=255)
                                                          cTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagca                              <  1:3570753/93‑1 (MQ=255)
                                                          cTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagca                              <  1:1141550/93‑1 (MQ=255)
                                                           tGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             <  2:1898911/93‑1 (MQ=255)
                                                           tGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcac                             >  1:4643607/1‑92 (MQ=255)
                                                             gcgcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCg                           <  1:2793717/93‑1 (MQ=255)
                                                             gcgcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCg                           <  1:277163/93‑1 (MQ=255)
                                                               gcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  2:5492658/93‑1 (MQ=255)
                                                                 aCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       <  1:1557216/93‑1 (MQ=255)
                                                                 aCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       <  1:317197/93‑1 (MQ=255)
                                                                 aCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       <  1:474099/93‑1 (MQ=255)
                                                                  cTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAACCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                      <  1:6105849/93‑1 (MQ=255)
                                                                  cTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTTCCGCTGGCGAACTGCTGTTAGCTAGCCGCAACCGCTTAAGCAGCGGtgtg                      >  2:829721/1‑93 (MQ=255)
                                                                  cTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                      >  2:317153/1‑93 (MQ=255)
                                                                   tGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:4407513/1‑93 (MQ=255)
                                                                   tGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     <  1:2561619/93‑1 (MQ=255)
                                                                    ggcgggcgGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGcc                    <  1:5043451/93‑1 (MQ=255)
                                                                        ggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  2:4051580/1‑93 (MQ=255)
                                                                         gcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               >  2:5900700/1‑93 (MQ=255)
                                                                         gcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  1:4020212/93‑1 (MQ=255)
                                                                           gggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCg             >  1:849929/1‑93 (MQ=255)
                                                                            ggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGc            <  2:4539759/93‑1 (MQ=255)
                                                                             gcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           >  2:1290178/1‑93 (MQ=255)
                                                                              cAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          >  2:5004727/1‑93 (MQ=255)
                                                                              cAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  1:5551298/93‑1 (MQ=255)
                                                                               aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  2:125670/1‑93 (MQ=255)
                                                                               aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  1:5416965/1‑93 (MQ=255)
                                                                               aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         <  2:4660585/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:339460/1‑93 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       >  1:2077315/1‑93 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       >  2:6225757/1‑93 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:2351761/93‑1 (MQ=255)
                                                                                   tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     <  2:5456758/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  2:1784173/1‑93 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:5322742/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  1:2102212/1‑93 (MQ=255)
                                                                                     tttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCt   <  2:3616314/93‑1 (MQ=255)
                                                                                         |                                                                                         
CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208242‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423
                                                                                             |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCG                                                                                              <  SRR4302533.5469494/94‑1 (MQ=60)
 TGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGC                                                                                             <  SRR4302533.2612732/94‑1 (MQ=60)
  GGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCA                                                                                            <  SRR4302533.3915909/94‑1 (MQ=60)
    CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAG                                                                                          <  SRR4302533.3720091/94‑1 (MQ=60)
     CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGC                                                                                         <  SRR4302533.5550514/94‑1 (MQ=60)
     CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGC                                                                                         <  SRR4302533.3723904/94‑1 (MQ=60)
        GAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGC                                                                                      >  SRR4302533.3129173/1‑94 (MQ=60)
        GAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATGTTTCGCAAGCAGC                                                                                      >  SRR4302533.3195836/1‑94 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCAAATTGTTTCGCAAGCAGCA                                                                                     >  SRR4302533.5392422/1‑94 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCAAATTGTTTCGCAAGCAGCA                                                                                     >  SRR4302533.4238159/1‑94 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCA                                                                                     >  SRR4302533.3869114/1‑94 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCA                                                                                     >  SRR4302533.474399/1‑94 (MQ=60)
          AGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAA                                                                                    >  SRR4302533.3534497/1‑94 (MQ=60)
           GTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAA                                                                                   >  SRR4302533.4586586/1‑94 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGGAAGCAGCAAAAC                                                                                 >  SRR4302533.6126218/1‑94 (MQ=60)
              AACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATGTTTCGCCAGCAGAAAAACG                                                                                >  SRR4302533.116754/1‑94 (MQ=60)
               ACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGC                                                                               <  SRR4302533.4582595/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302533.6112958/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302533.899817/94‑1 (MQ=60)
                  GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302533.5614555/94‑1 (MQ=60)
                  GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGG                                                                            >  SRR4302533.910906/1‑94 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302533.4936143/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302533.2456618/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302533.6264310/94‑1 (MQ=60)
                    AAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCCCAAGCAGGCAAACGCAGTGC                                                                          >  SRR4302533.3113671/1‑94 (MQ=60)
                       ACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGA                                                                       <  SRR4302533.622778/94‑1 (MQ=60)
                        cgccCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302533.1759800/90‑1 (MQ=60)
                         TCACCGCCAGCTACGCGTGGGTTGTTTAGTGTTCAGCCTGGCGCCCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATG                                                                     <  SRR4302533.2317002/94‑1 (MQ=60)
                          CCCCGGCAGCTACGCGGCGGCTGTTTAGTTTTCGGGCTGGCGCACTGGCGTGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGG                                                                    <  SRR4302533.3082353/94‑1 (MQ=60)
                           ACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTTTTTCGCCAGCCGCAAAACACAGTGCCGcgcgc                                                                   >  SRR4302533.6195388/1‑89 (MQ=60)
                              GGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAG                                                                <  SRR4302533.5674804/94‑1 (MQ=60)
                              GGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAG                                                                >  SRR4302533.209038/1‑94 (MQ=60)
                               GCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGC                                                               <  SRR4302533.6167942/94‑1 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             >  SRR4302533.578235/1‑94 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             >  SRR4302533.5897549/1‑94 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           <  SRR4302533.5579474/94‑1 (MQ=60)
                                    TACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTG                                                          <  SRR4302533.4342517/94‑1 (MQ=60)
                                      CGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTT                                                        <  SRR4302533.5671371/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAATTGTTTCGGAAGCAGCAAAACGCAGATCCGATGGCGGGCTCCTGTTAG                                                      >  SRR4302533.1175198/1‑94 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGGGCTTGTGTTAG                                                      >  SRR4302533.1587257/1‑94 (MQ=60)
                                         GTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCCAGCAGCAAAACCCAGTGCCGACTGCGAGCTGCTTTTAGC                                                     >  SRR4302533.2858045/1‑94 (MQ=60)
                                         GTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCCAAACGCAGTGCCGATGGCGAGCTGCTGTTAGC                                                     >  SRR4302533.2810044/1‑94 (MQ=60)
                                          TCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCT                                                    <  SRR4302533.6023672/94‑1 (MQ=60)
                                           CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCCGGCAAATTGTTTCGCAAGCAACAAAACGCAGTatcgaaggcgagacggtgttcagta                                                   >  SRR4302533.725484/1‑69 (MQ=60)
                                            GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAC                                                  >  SRR4302533.4231740/1‑94 (MQ=60)
                                            GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCTAC                                                  >  SRR4302533.286061/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                >  SRR4302533.1991373/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                >  SRR4302533.3841060/1‑94 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCG                                               <  SRR4302533.4428980/94‑1 (MQ=60)
                                                  TTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAC                                            >  SRR4302533.5065060/1‑94 (MQ=60)
                                                   TAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAACCGCAAC                                           >  SRR4302533.4777831/1‑94 (MQ=60)
                                                    AGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACC                                          <  SRR4302533.1662578/94‑1 (MQ=60)
                                                      TTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGC                                        >  SRR4302533.2243778/1‑94 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       >  SRR4302533.3697603/1‑94 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       >  SRR4302533.6213681/1‑94 (MQ=60)
                                                          CAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCCACCGCTTAA                                    >  SRR4302533.246750/1‑94 (MQ=60)
                                                           AGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAG                                   <  SRR4302533.5363268/94‑1 (MQ=60)
                                                            GGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGC                                  <  SRR4302533.2353498/94‑1 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302533.3492560/94‑1 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302533.3459734/94‑1 (MQ=60)
                                                              CTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAG                                <  SRR4302533.3592757/94‑1 (MQ=60)
                                                              CTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAG                                <  SRR4302533.2321439/94‑1 (MQ=60)
                                                              CTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAG                                <  SRR4302533.1148353/94‑1 (MQ=60)
                                                              CTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAC                                >  SRR4302533.4671740/1‑94 (MQ=60)
                                                               TGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCCACCGCTTAAGCAGC                               >  SRR4302533.2442550/1‑94 (MQ=60)
                                                               TGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGC                               <  SRR4302533.1180508/94‑1 (MQ=60)
                                                               TGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGC                               >  SRR4302533.5481152/1‑94 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              <  SRR4302533.4200032/94‑1 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              <  SRR4302533.2961666/94‑1 (MQ=60)
                                                                 GCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGT                             <  SRR4302533.638732/94‑1 (MQ=60)
                                                                 GCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGT                             <  SRR4302533.2810902/94‑1 (MQ=60)
                                                                 GCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGT                             <  SRR4302533.278854/94‑1 (MQ=60)
                                                                    CACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGT                          >  SRR4302533.2840310/1‑94 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302533.2127582/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302533.319138/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302533.476929/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302533.1566588/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         >  SRR4302533.588536/1‑94 (MQ=60)
                                                                      CTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAACCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGC                        <  SRR4302533.6142719/94‑1 (MQ=60)
                                                                       TGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCC                       <  SRR4302533.2577391/94‑1 (MQ=60)
                                                                        GGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCG                      <  SRR4302533.5073974/94‑1 (MQ=60)
                                                                             GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC                 <  SRR4302533.3167949/94‑1 (MQ=60)
                                                                             GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC                 <  SRR4302533.2775413/94‑1 (MQ=60)
                                                                             GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC                 <  SRR4302533.4044693/94‑1 (MQ=60)
                                                                              CGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCG                >  SRR4302533.854905/1‑94 (MQ=60)
                                                                               GGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGC               >  SRR4302533.1005180/1‑94 (MQ=60)
                                                                                 GCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT             <  SRR4302533.2344489/94‑1 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            <  SRR4302533.5584821/94‑1 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            >  SRR4302533.5449550/1‑94 (MQ=60)
                                                                                   AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA           >  SRR4302533.341530/1‑94 (MQ=60)
                                                                                    AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          >  SRR4302533.2090100/1‑94 (MQ=60)
                                                                                    AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302533.2364888/94‑1 (MQ=60)
                                                                                     ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302533.2366310/94‑1 (MQ=60)
                                                                                       TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       >  SRR4302533.4520275/1‑94 (MQ=60)
                                                                                       TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       >  SRR4302533.2115175/1‑94 (MQ=60)
                                                                                        GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCACTGTCGCCTGATGCCG      >  SRR4302533.2112779/1‑94 (MQ=60)
                                                                                          TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302533.5162829/94‑1 (MQ=60)
                                                                                            CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  SRR4302533.3334931/1‑94 (MQ=60)
                                                                                             |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑