Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG99.5% 637.0 / ‑5.6 205S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (64/140);  total (65/140)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 235 total aligned reads displayed.

CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208243‑2208420
                                                                                         |                                                                                         
  cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAagca                                                                                     <  2:159345/93‑1 (MQ=255)
   gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAagcag                                                                                    >  1:9124014/1‑93 (MQ=255)
    aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTTTTTCGGCagcagc                                                                                   >  2:8218874/1‑93 (MQ=255)
    aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTGGTTCGCAagcagc                                                                                   >  2:9414601/1‑93 (MQ=255)
     aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAacagca                                                                                  >  2:9029757/1‑93 (MQ=255)
      gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑ACAATTTTTCGCAAGCCGCaa                                                                                 >  2:2441801/1‑93 (MQ=255)
      gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaa                                                                                 <  2:7000662/93‑1 (MQ=255)
        gAACAGGACACCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  2:6300929/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  1:3137874/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGCTTCGCAAGCAGCaaaa                                                                               <  2:8112529/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAATTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  1:4940581/93‑1 (MQ=255)
         aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAACCACCAAAAc                                                                              >  1:8532488/1‑93 (MQ=255)
            cggAAAACTCCCCTGCAGCTACGCGTCGGCTGTTTAATTTTCAGGCTGGCGCACTGGCCGGCGGGG‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  2:2836198/92‑1 (MQ=255)
            aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           >  2:934382/1‑93 (MQ=255)
            aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  1:8740051/93‑1 (MQ=255)
                aaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGc                                                                       <  1:6141478/93‑1 (MQ=255)
                aaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGc                                                                       <  2:893898/93‑1 (MQ=255)
                  aCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCg                                                                     <  2:8598364/93‑1 (MQ=255)
                   cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:8115187/93‑1 (MQ=255)
                   cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  2:9747114/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCATTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  2:3382718/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  1:5642769/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  1:7946284/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGGTTCGCCAGCAGCAAAAACCAGTGCCCAt                                                                   >  1:4731511/1‑93 (MQ=255)
                      aCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATgg                                                                 >  2:1093529/1‑93 (MQ=255)
                       ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGc                                                                <  2:6024162/93‑1 (MQ=255)
                         ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              >  1:1066914/1‑93 (MQ=255)
                         ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              <  2:6669123/93‑1 (MQ=255)
                              cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑GAAATGTTTCGCAAGCAGCAAAAGGCGGTGCCGAGGGGGAgctgg                                                         >  1:7556741/1‑92 (MQ=255)
                               tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGCgctgct                                                        >  2:8366108/1‑93 (MQ=255)
                               tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgct                                                        >  1:329743/1‑93 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGctgctg                                                       <  1:3869708/93‑1 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGctgctg                                                       <  1:3852785/93‑1 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAGGGCGAGctgctg                                                       >  2:4365723/1‑93 (MQ=255)
                                 cgcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGt                                                      >  2:8571658/1‑93 (MQ=255)
                                  gcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATTGTTT‑GCAAGCAGCAAAACGCCGTGCCGACGGGGAGCTGCTGtt                                                     >  1:5576136/1‑93 (MQ=255)
                                  gcgTCGGCTGTTTAGTGTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGtt                                                     <  1:6632416/93‑1 (MQ=255)
                                     tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGAAGCAAAACGCAGTGCCGATTGCGAGCTGCTGTTAGc                                                  >  1:6880025/1‑93 (MQ=255)
                                      cGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCt                                                 <  2:5676180/93‑1 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCCAAACGCAGTGCCGATGGCGGGCTGCTGTTAGCTa                                                >  1:6500426/1‑93 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  1:3593222/1‑93 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  2:7206330/1‑93 (MQ=255)
                                        gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               <  1:2496114/93‑1 (MQ=255)
                                         cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGGAAGCAGCAAAACGCAGGGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:3860993/1‑93 (MQ=255)
                                         cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:723960/93‑1 (MQ=255)
                                          tGTTTAGTTTTCAGGCTGGCGCACTGGCGTGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  1:1477972/93‑1 (MQ=255)
                                          tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  2:3537189/93‑1 (MQ=255)
                                           gattAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCg                                            <  2:4761013/91‑1 (MQ=255)
                                           gTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCCGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCg                                            >  1:4549502/1‑93 (MQ=255)
                                           gTTTAGTTTTCAGGCTCGCGCACTGGCGGGCGGGG‑AAATTGATTTGCTAGCAGCAAAACGCAGCGCCGATCGCGTGCTGCCGTTAGATACCCg                                            >  2:2122568/1‑93 (MQ=255)
                                              tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  1:5278764/93‑1 (MQ=255)
                                              tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  1:4677043/93‑1 (MQ=255)
                                                gTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAcc                                       <  2:6420610/93‑1 (MQ=255)
                                                  tttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     <  1:4046656/93‑1 (MQ=255)
                                                  tttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     >  1:538882/1‑93 (MQ=255)
                                                   ttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    >  1:1898626/1‑93 (MQ=255)
                                                   ttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    >  1:9365204/1‑93 (MQ=255)
                                                     cAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTa                                  <  1:6401934/93‑1 (MQ=255)
                                                     cAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTa                                  <  2:9694657/93‑1 (MQ=255)
                                                       ggCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAag                                >  2:4276407/1‑93 (MQ=255)
                                                        gCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               >  2:2619308/1‑93 (MQ=255)
                                                        gCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  2:7572872/93‑1 (MQ=255)
                                                         cTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTATCCGCAACCGCTTAagca                              >  2:10017071/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            >  1:2365436/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  1:2438058/93‑1 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            >  2:4324313/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:9911846/93‑1 (MQ=255)
                                                           ggCGCACGGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:9628654/93‑1 (MQ=255)
                                                            gcgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCg                           >  2:4412351/1‑93 (MQ=255)
                                                             cgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:1807500/93‑1 (MQ=255)
                                                             cgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:5846753/93‑1 (MQ=255)
                                                              gcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         >  2:4102802/1‑93 (MQ=255)
                                                                aCTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       <  2:7477450/93‑1 (MQ=255)
                                                                 cTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                      <  1:3933004/93‑1 (MQ=255)
                                                                  tGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:4751612/1‑93 (MQ=255)
                                                                      gggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCt                 <  2:5901034/93‑1 (MQ=255)
                                                                       ggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  2:117693/1‑93 (MQ=255)
                                                                       ggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  1:5098808/1‑93 (MQ=255)
                                                                          gggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCg             >  2:5006326/1‑93 (MQ=255)
                                                                            gc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           <  2:9124013/93‑1 (MQ=255)
                                                                            gc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           <  1:5887784/93‑1 (MQ=255)
                                                                             c‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  1:222496/93‑1 (MQ=255)
                                                                               aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         <  2:8744739/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:1792506/1‑93 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:3068104/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  2:3274506/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:9058443/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:4092064/93‑1 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:6272020/93‑1 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  2:3126578/93‑1 (MQ=255)
                                                                                  ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  2:8049913/1‑93 (MQ=255)
                                                                                  ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      <  2:7784798/93‑1 (MQ=255)
                                                                                   tctttCGCAAGCAGCAAAGCGCAGTGCCGATGGCGATCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     <  2:1836515/91‑1 (MQ=255)
                                                                                   tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     >  2:9002739/1‑93 (MQ=255)
                                                                                   tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     <  1:2927959/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCTGCTGTCGCCTGATGcc    <  2:9556962/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  1:6758241/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  1:3564898/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:6791176/93‑1 (MQ=255)
                                                                                      ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:1343194/93‑1 (MQ=255)
                                                                                         |                                                                                         
CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208243‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423
                                                                                              |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCG                                                                                              <  SRR4302540.4200054/94‑1 (MQ=60)
    CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAAATGTTTTGCAAG                                                                                          >  SRR4302540.4118225/1‑94 (MQ=60)
     CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTGTTTCGCAAGC                                                                                         >  SRR4302540.5731348/1‑94 (MQ=60)
      GCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCA                                                                                        <  SRR4302540.2467656/94‑1 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAG                                                                                       >  SRR4302540.9233684/1‑94 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAG                                                                                       <  SRR4302540.8205596/94‑1 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAAATGTTTCGCAAGCAGCA                                                                                     >  SRR4302540.239546/1‑94 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCCAGCAGCAAAAC                                                                                 >  SRR4302540.5670530/1‑94 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302540.3172099/94‑1 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302540.8738619/94‑1 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAACCACCAAAAC                                                                                 >  SRR4302540.8631621/1‑94 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAATTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302540.4993899/94‑1 (MQ=60)
               ACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGC                                                                               <  SRR4302540.3773859/94‑1 (MQ=60)
               ACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGC                                                                               <  SRR4302540.6818405/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302540.2915699/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302540.4733688/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302540.4788598/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302540.8841513/94‑1 (MQ=60)
                  GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302540.4275444/94‑1 (MQ=60)
                  GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302540.678649/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑CAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           >  SRR4302540.9241462/1‑94 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302540.1215547/94‑1 (MQ=60)
                     AAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCC                                                                         <  SRR4302540.6209878/94‑1 (MQ=60)
                      AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCG                                                                        >  SRR4302540.773273/1‑94 (MQ=60)
                      AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCG                                                                        <  SRR4302540.6263635/94‑1 (MQ=60)
                       ACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTGGTTCTCAAGCAGCAAAACGCAGgggcgg                                                                       >  SRR4302540.1982719/1‑88 (MQ=60)
                       ACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTTTTTCGCAAGCAGCAAAACGCAGTGCCGG                                                                       >  SRR4302540.9866370/1‑94 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGGCGCCAAACGCAGTGCCGAT                                                                      >  SRR4302540.6081954/1‑94 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302540.8209373/94‑1 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGGTTCGCCAGCAGCAAAAACCAGTGCCCAT                                                                      >  SRR4302540.4782039/1‑94 (MQ=60)
                         TCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATG                                                                     <  SRR4302540.5704875/94‑1 (MQ=60)
                         TCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATG                                                                     <  SRR4302540.8038905/94‑1 (MQ=60)
                            CCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCG                                                                  <  SRR4302540.8201912/94‑1 (MQ=60)
                             CGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGA                                                                 >  SRR4302540.1077787/1‑94 (MQ=60)
                              GGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGACGGCGAG                                                                >  SRR4302540.6535443/1‑94 (MQ=60)
                                CAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCT                                                              <  SRR4302540.3991929/94‑1 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             >  SRR4302540.3279888/1‑94 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             >  SRR4302540.3194620/1‑94 (MQ=60)
                                  GCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑GAAATGTTTCGCAAGCAGCAAAAGGCGGTGCCGAGGGGGAGCTGG                                                            >  SRR4302540.7641480/1‑94 (MQ=60)
                                  GCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGC                                                            <  SRR4302540.5367729/94‑1 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           >  SRR4302540.333050/1‑94 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           >  SRR4302540.5013268/1‑94 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGCTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           >  SRR4302540.7087841/1‑94 (MQ=60)
                                    TACGCGTCGGGTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTG                                                          <  SRR4302540.8596862/94‑1 (MQ=60)
                                     ACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGT                                                         <  SRR4302540.3911774/94‑1 (MQ=60)
                                     ACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGT                                                         <  SRR4302540.3959954/94‑1 (MQ=60)
                                     ACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGT                                                         <  SRR4302540.3894672/94‑1 (MQ=60)
                                      CGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATTGTTT‑GCAAGCAGCAAAACGCCGTGCCGACGGGGAGCTGCTGTT                                                        >  SRR4302540.5637527/1‑94 (MQ=60)
                                       GCGTCGGCTGTTTAGTGTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTA                                                       <  SRR4302540.6705826/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302540.5813340/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302540.4083297/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGACGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302540.8986131/94‑1 (MQ=60)
                                         GTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGAAGCAAAACGCAGTGCCGATTGCGAGCTGCTGTTAGC                                                     >  SRR4302540.6956287/1‑94 (MQ=60)
                                           CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCCAAACGCAGTGCCGATGGCGGGCTGCTGTTAGCTA                                                   >  SRR4302540.6572692/1‑94 (MQ=60)
                                           CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTA                                                   >  SRR4302540.3632474/1‑94 (MQ=60)
                                             GCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACC                                                 <  SRR4302540.2523014/94‑1 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                <  SRR4302540.731718/94‑1 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGTGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCG                                               <  SRR4302540.1494154/94‑1 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCCGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCG                                               >  SRR4302540.4598712/1‑94 (MQ=60)
                                                 TTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCA                                             >  SRR4302540.7553560/1‑94 (MQ=60)
                                                  TTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAA                                            >  SRR4302540.5426556/1‑94 (MQ=60)
                                                   TAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAC                                           <  SRR4302540.4727085/94‑1 (MQ=60)
                                                   TAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAC                                           >  SRR4302540.7558430/1‑94 (MQ=60)
                                                   TAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAC                                           <  SRR4302540.5336413/94‑1 (MQ=60)
                                                      TTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGC                                        >  SRR4302540.544714/1‑94 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       <  SRR4302540.4090290/94‑1 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       >  SRR4302540.1919400/1‑94 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       >  SRR4302540.9476718/1‑94 (MQ=60)
                                                        TTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTT                                      <  SRR4302540.2389781/94‑1 (MQ=60)
                                                         ttcgggcggggccccgggGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTA                                     <  SRR4302540.6483324/76‑1 (MQ=60)
                                                          CAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAA                                    <  SRR4302540.6473191/94‑1 (MQ=60)
                                                           AGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAG                                   <  SRR4302540.3474797/94‑1 (MQ=60)
                                                           AGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAC                                   >  SRR4302540.693992/1‑94 (MQ=60)
                                                            GGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGC                                  >  SRR4302540.5283100/1‑94 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302540.3044040/94‑1 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302540.6598568/94‑1 (MQ=60)
                                                             GCTGGCGCACGGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302540.6424709/94‑1 (MQ=60)
                                                               TGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGC                               >  SRR4302540.2390906/1‑94 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              <  SRR4302540.2464312/94‑1 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              >  SRR4302540.8888324/1‑94 (MQ=60)
                                                                    CACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGT                          <  SRR4302540.3697867/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATTGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         >  SRR4302540.4786887/1‑94 (MQ=60)
                                                                     ACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302540.1439367/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302540.551013/94‑1 (MQ=60)
                                                                      CTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGC                        <  SRR4302540.3975518/94‑1 (MQ=60)
                                                                      CTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGC                        <  SRR4302540.9197244/94‑1 (MQ=60)
                                                                       TGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGATACCCGCAACCGCTTAAGCAGCGTTGTGCC                       >  SRR4302540.4247367/1‑94 (MQ=60)
                                                                        GGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCG                      >  SRR4302540.270862/1‑94 (MQ=60)
                                                                           GGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTG                   >  SRR4302540.5153986/1‑94 (MQ=60)
                                                                                 GC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT             <  SRR4302540.5952834/94‑1 (MQ=60)
                                                                                  C‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            >  SRR4302540.390818/1‑94 (MQ=60)
                                                                                  C‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            <  SRR4302540.224649/94‑1 (MQ=60)
                                                                                    AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA           >  SRR4302540.1811986/1‑94 (MQ=60)
                                                                                    AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA           >  SRR4302540.1811987/1‑94 (MQ=60)
                                                                                    AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA           >  SRR4302540.1721448/1‑94 (MQ=60)
                                                                                     AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302540.9647318/94‑1 (MQ=60)
                                                                                     AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302540.4136214/94‑1 (MQ=60)
                                                                                     AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302540.9167194/94‑1 (MQ=60)
                                                                                     AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302540.3101765/94‑1 (MQ=60)
                                                                                      ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302540.4667833/94‑1 (MQ=60)
                                                                                      ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302540.6341804/94‑1 (MQ=60)
                                                                                        TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       <  SRR4302540.2960064/94‑1 (MQ=60)
                                                                                         GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302540.3603842/94‑1 (MQ=60)
                                                                                         GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302540.3603844/94‑1 (MQ=60)
                                                                                         GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302540.311846/94‑1 (MQ=60)
                                                                                         GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      >  SRR4302540.2122967/1‑94 (MQ=60)
                                                                                         GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302540.6832929/94‑1 (MQ=60)
                                                                                          TTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG     >  SRR4302540.2271575/1‑94 (MQ=60)
                                                                                          TTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG     <  SRR4302540.1502693/94‑1 (MQ=60)
                                                                                           TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302540.6386041/94‑1 (MQ=60)
                                                                                           TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302540.1357274/94‑1 (MQ=60)
                                                                                           TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    >  SRR4302540.5090651/1‑94 (MQ=60)
                                                                                             CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTTTCGCCTGATGCCTGGCA  >  SRR4302540.7057979/1‑94 (MQ=60)
                                                                                              |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑