Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,218,691 A→G V440A (GTG→GCG)  bglX ← beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,218,6910AG100.0% 624.1 / NA 195V440A (GTG→GCG) bglXbeta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (107/88);  total (107/88)

Only 100 of 203 total aligned reads displayed.

ATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCC  >  NC_000913/2218601‑2218780
                                                                                          |                                                                                         
 tGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCgg                                                                                        >  2:197206/1‑93 (MQ=255)
 tGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCgg                                                                                        <  2:4582652/93‑1 (MQ=255)
  tccTTTGTCACGGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCCCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGa                                                                                       <  2:5985301/92‑1 (MQ=255)
   ccTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGAt                                                                                      >  2:4271065/1‑93 (MQ=255)
    cTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGAtt                                                                                     >  2:2224715/1‑93 (MQ=255)
     tttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTg                                                                                    >  2:3372484/1‑93 (MQ=255)
     tttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTg                                                                                    >  1:5101586/1‑93 (MQ=255)
     tttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTg                                                                                    >  1:1367156/1‑93 (MQ=255)
     tttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTg                                                                                    <  1:4403988/93‑1 (MQ=255)
      ttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGa                                                                                   <  1:585945/93‑1 (MQ=255)
      ttGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGa                                                                                   >  2:2358569/1‑93 (MQ=255)
       tGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGAt                                                                                  <  2:5726498/93‑1 (MQ=255)
       tGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGAt                                                                                  <  2:5892382/93‑1 (MQ=255)
       tGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGAt                                                                                  >  1:1048435/1‑93 (MQ=255)
        gTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATc                                                                                 >  1:5044235/1‑93 (MQ=255)
          cACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCgg                                                                               >  2:5385379/1‑93 (MQ=255)
          cACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCgg                                                                               >  2:4518742/1‑93 (MQ=255)
            cTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCa                                                                             >  2:1679116/1‑93 (MQ=255)
             tGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCaa                                                                            >  2:3165741/1‑93 (MQ=255)
              ggTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAac                                                                           >  1:2514699/1‑93 (MQ=255)
               gTAACGTTCGCCACTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAaca                                                                          <  1:5413892/93‑1 (MQ=255)
                 aaCGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACAcc                                                                        >  1:3171900/1‑93 (MQ=255)
                 aaCGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTCTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACAcc                                                                        <  2:5133728/93‑1 (MQ=255)
                   cGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCgg                                                                      <  1:2102740/93‑1 (MQ=255)
                   cGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCgg                                                                      <  1:1784191/93‑1 (MQ=255)
                   cGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCgg                                                                      <  2:82819/93‑1 (MQ=255)
                    gTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGc                                                                     >  2:1584870/1‑93 (MQ=255)
                    gTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGc                                                                     >  1:6187049/1‑93 (MQ=255)
                        gCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCg                                                                 >  2:3933353/1‑93 (MQ=255)
                        gCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCg                                                                 >  1:3484893/1‑93 (MQ=255)
                          cccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCggc                                                               >  1:4377298/1‑92 (MQ=255)
                          cccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGa                                                               <  2:3838270/93‑1 (MQ=255)
                          cccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGa                                                               <  1:2955677/93‑1 (MQ=255)
                           ccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGAc                                                              >  2:2069042/1‑93 (MQ=255)
                           ccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGAc                                                              >  1:2403886/1‑93 (MQ=255)
                           ccTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGAc                                                              >  2:4393622/1‑93 (MQ=255)
                             tttGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCa                                                            >  2:5468025/1‑93 (MQ=255)
                             tttGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCa                                                            >  1:3079945/1‑93 (MQ=255)
                               tGGCATACAGCACTTTACCGTTTTCCCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGc                                                          <  1:5953435/93‑1 (MQ=255)
                               tGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGc                                                          <  1:4423892/93‑1 (MQ=255)
                               tGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGc                                                          <  2:1124930/93‑1 (MQ=255)
                               tGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGc                                                          <  1:3006035/93‑1 (MQ=255)
                                ggCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCt                                                         <  2:2140399/93‑1 (MQ=255)
                                 gCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTg                                                        >  1:330226/1‑93 (MQ=255)
                                  cATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGc                                                       >  2:1909934/1‑93 (MQ=255)
                                     aCAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCa                                                    <  1:965946/93‑1 (MQ=255)
                                     aCAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCa                                                    >  1:2788115/1‑93 (MQ=255)
                                      cAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAt                                                   >  2:1755329/1‑93 (MQ=255)
                                       aGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCACAtc                                                  >  2:464801/1‑93 (MQ=255)
                                        gCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAtca                                                 <  1:6127561/93‑1 (MQ=255)
                                         cACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAtcac                                                <  2:1808406/93‑1 (MQ=255)
                                           cTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAtcacgt                                              >  2:1518585/1‑93 (MQ=255)
                                              tACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAtcacgtcac                                           <  1:2326925/93‑1 (MQ=255)
                                                ccGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATcacgtcacgt                                         >  2:6245931/1‑93 (MQ=255)
                                                ccGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATcacgtcacgt                                         >  2:5805571/1‑93 (MQ=255)
                                                ccGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATcacgtcacgt                                         >  1:1513426/1‑93 (MQ=255)
                                                 cGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGtt                                        >  1:737159/1‑93 (MQ=255)
                                                  gTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGttt                                       >  2:1097883/1‑93 (MQ=255)
                                                   ttttCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTa                                      >  2:4209659/1‑93 (MQ=255)
                                                   ttttCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTa                                      <  1:465093/93‑1 (MQ=255)
                                                    tttCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTAc                                     <  2:5633594/93‑1 (MQ=255)
                                                    tttCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTAc                                     <  2:1363135/93‑1 (MQ=255)
                                                        aCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTc                                 <  1:5126043/93‑1 (MQ=255)
                                                        aCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTc                                 <  1:4181316/93‑1 (MQ=255)
                                                        aCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGATGCGGACCAGCTGCCCATCACGTCACGTTTACTGTc                                 >  1:1914433/1‑93 (MQ=255)
                                                         ccGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTcc                                <  1:3755261/93‑1 (MQ=255)
                                                         ccGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTcc                                >  2:5561960/1‑93 (MQ=255)
                                                           gACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGc                              <  1:854974/93‑1 (MQ=255)
                                                           gACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGc                              >  2:4803349/1‑93 (MQ=255)
                                                            ccGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACCCCGGCTGCGGACCAGCGGCCTATGACGGCCCGTTTACTGTCCGcc                             <  2:1862514/92‑1 (MQ=255)
                                                            aCGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGcc                             >  1:2148261/1‑93 (MQ=255)
                                                            aCGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGcc                             >  1:4464872/1‑93 (MQ=255)
                                                            aCGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGcc                             >  1:4931771/1‑93 (MQ=255)
                                                             cGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTTCTGTCTGCCa                            >  1:1157405/1‑93 (MQ=255)
                                                              ggCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACCCCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAg                           <  2:2435881/93‑1 (MQ=255)
                                                              ggCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAg                           <  2:5118753/93‑1 (MQ=255)
                                                              ggCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAg                           >  1:1385157/1‑93 (MQ=255)
                                                               gCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGCCCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGt                          <  2:1335444/93‑1 (MQ=255)
                                                                cATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTg                         <  1:4331538/93‑1 (MQ=255)
                                                                cATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTg                         <  2:4062493/93‑1 (MQ=255)
                                                                 aTTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTgg                        >  2:4017264/1‑93 (MQ=255)
                                                                  tttttAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGc                       >  1:1942904/1‑93 (MQ=255)
                                                                  tttttAATCCCGGTCAGTAC‑GTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGcc                      >  1:5750821/1‑93 (MQ=255)
                                                                   ttttAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGcc                      >  2:368225/1‑93 (MQ=255)
                                                                         tCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAacca                <  2:5036481/93‑1 (MQ=255)
                                                                         tCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAacca                >  1:3602209/1‑93 (MQ=255)
                                                                         tCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAacca                >  1:2073184/1‑93 (MQ=255)
                                                                         tCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAacca                >  2:5267221/1‑93 (MQ=255)
                                                                          cccGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAaccac               <  2:3560948/93‑1 (MQ=255)
                                                                           ccGGTCAGTACGGTCGCGGATTGATCGGCAACACGGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAaccacc              <  1:3446141/93‑1 (MQ=255)
                                                                            cGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCg             >  2:2789878/1‑93 (MQ=255)
                                                                              gTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCa           >  1:5472928/1‑93 (MQ=255)
                                                                               tCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCaa          >  1:4802712/1‑93 (MQ=255)
                                                                                 aGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtg        <  1:5676988/93‑1 (MQ=255)
                                                                                 aGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtg        <  1:3488936/93‑1 (MQ=255)
                                                                                  gTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtgg       >  1:5386667/1‑93 (MQ=255)
                                                                                   tACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtggt      <  1:5275696/93‑1 (MQ=255)
                                                                                   tACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtggt      <  1:1246333/93‑1 (MQ=255)
                                                                                   tACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAAtggt      <  2:1992182/93‑1 (MQ=255)
                                                                                       gTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGcc  >  2:6087194/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCC  >  NC_000913/2218601‑2218780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGAT  >  NC_000913/2218598‑2218783
                                                                                             |                                                                                            
ATAATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCG                                                                                              >  SRR4302533.3729188/1‑94 (MQ=60)
 TAATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGC                                                                                             >  SRR4302533.3799198/1‑94 (MQ=60)
  AATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCG                                                                                            >  SRR4302533.3063388/1‑94 (MQ=60)
  AATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCG                                                                                            <  SRR4302533.5801654/94‑1 (MQ=60)
   ATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGG                                                                                           >  SRR4302533.2029869/1‑94 (MQ=60)
    TGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGA                                                                                          <  SRR4302533.4884724/94‑1 (MQ=60)
     GCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCAATTTTAATCCCGGTCAGTACGGTCGCGGAT                                                                                         >  SRR4302533.443746/1‑94 (MQ=60)
      CCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATT                                                                                        >  SRR4302533.5125948/1‑94 (MQ=60)
       CTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTG                                                                                       >  SRR4302533.5132400/1‑94 (MQ=60)
       CTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTG                                                                                       >  SRR4302533.1375376/1‑94 (MQ=60)
       CTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTG                                                                                       >  SRR4302533.1379988/1‑94 (MQ=60)
       CTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTG                                                                                       <  SRR4302533.3392275/94‑1 (MQ=60)
        TTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGA                                                                                      <  SRR4302533.4430580/94‑1 (MQ=60)
         TTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGAT                                                                                     >  SRR4302533.1054596/1‑94 (MQ=60)
         TTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGAT                                                                                     <  SRR4302533.589400/94‑1 (MQ=60)
          TGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATC                                                                                    <  SRR4302533.5049154/94‑1 (MQ=60)
          TGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATC                                                                                    >  SRR4302533.5074758/1‑94 (MQ=60)
           GTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCG                                                                                   >  SRR4302533.1637276/1‑94 (MQ=60)
           GTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCG                                                                                   <  SRR4302533.2147698/94‑1 (MQ=60)
              ACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCA                                                                                >  SRR4302533.2992158/1‑94 (MQ=60)
               CTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAA                                                                               >  SRR4302533.4269273/1‑94 (MQ=60)
               CTGGTAACGCTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAA                                                                               <  SRR4302533.946808/94‑1 (MQ=60)
                TGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAAC                                                                              >  SRR4302533.2530194/1‑94 (MQ=60)
                 GGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACA                                                                             >  SRR4302533.3189182/1‑94 (MQ=60)
                 GGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACA                                                                             <  SRR4302533.3673909/94‑1 (MQ=60)
                  GTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACAC                                                                            >  SRR4302533.4855446/1‑94 (MQ=60)
                  GTAACGTTCGCCACTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACAC                                                                            <  SRR4302533.5446448/94‑1 (MQ=60)
                   TAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACC                                                                           >  SRR4302533.3191425/1‑94 (MQ=60)
                   TAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACC                                                                           >  SRR4302533.580013/1‑94 (MQ=60)
                    AACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCG                                                                          <  SRR4302533.4376873/94‑1 (MQ=60)
                    AACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCG                                                                          >  SRR4302533.2093371/1‑94 (MQ=60)
                      CGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGC                                                                        <  SRR4302533.2115707/94‑1 (MQ=60)
                      CGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGC                                                                        <  SRR4302533.1795039/94‑1 (MQ=60)
                      CGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGC                                                                        >  SRR4302533.6224186/1‑94 (MQ=60)
                       GTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCT                                                                       <  SRR4302533.6088761/94‑1 (MQ=60)
                         TCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGC                                                                     <  SRR4302533.5936259/94‑1 (MQ=60)
                          TGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCG                                                                    <  SRR4302533.1893345/94‑1 (MQ=60)
                          CGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCG                                                                    >  SRR4302533.3506383/1‑94 (MQ=60)
                          CGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCG                                                                    >  SRR4302533.4667356/1‑94 (MQ=60)
                            CCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGC                                                                  >  SRR4302533.4403725/1‑94 (MQ=60)
                             CCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGAC                                                                 <  SRR4302533.2973748/94‑1 (MQ=60)
                             CCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGAC                                                                 >  SRR4302533.2418732/1‑94 (MQ=60)
                               CTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGGCCA                                                               >  SRR4302533.235182/1‑94 (MQ=60)
                               CTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCA                                                               <  SRR4302533.3991757/94‑1 (MQ=60)
                               CTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCA                                                               >  SRR4302533.3098843/1‑94 (MQ=60)
                                TTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAG                                                              >  SRR4302533.482171/1‑94 (MQ=60)
                                TTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAG                                                              <  SRR4302533.1402114/94‑1 (MQ=60)
                                TTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAG                                                              >  SRR4302533.5325618/1‑94 (MQ=60)
                                TTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAG                                                              <  SRR4302533.440601/94‑1 (MQ=60)
                                TTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAG                                                              >  SRR4302533.2912535/1‑94 (MQ=60)
                                  TGGCATACAGCACTTTACCGTTTTCCCCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCT                                                            <  SRR4302533.5989448/94‑1 (MQ=60)
                                  TGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCT                                                            <  SRR4302533.3024422/94‑1 (MQ=60)
                                  TGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCT                                                            <  SRR4302533.4450635/94‑1 (MQ=60)
                                   GGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTG                                                           >  SRR4302533.332244/1‑94 (MQ=60)
                                   GGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTG                                                           <  SRR4302533.4476327/94‑1 (MQ=60)
                                       TACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCA                                                       >  SRR4302533.2805246/1‑94 (MQ=60)
                                        ACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCAT                                                      <  SRR4302533.971614/94‑1 (MQ=60)
                                           GCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCAC                                                   <  SRR4302533.6164491/94‑1 (MQ=60)
                                              CTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTC                                                >  SRR4302533.5453325/1‑94 (MQ=60)
                                                 TACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACG                                             <  SRR4302533.2341330/94‑1 (MQ=60)
                                                  ACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGT                                            >  SRR4302533.1522545/1‑94 (MQ=60)
                                                   CCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTT                                           >  SRR4302533.741498/1‑94 (MQ=60)
                                                    CGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTT                                          >  SRR4302533.1265289/1‑94 (MQ=60)
                                                    CGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTT                                          >  SRR4302533.1946633/1‑94 (MQ=60)
                                                     GTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGGCCAGCTGCCCATCACGTCACGTTTA                                         >  SRR4302533.3479023/1‑94 (MQ=60)
                                                     GTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTA                                         <  SRR4302533.5721756/94‑1 (MQ=60)
                                                     GTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTA                                         >  SRR4302533.6334575/1‑94 (MQ=60)
                                                      TTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTAC                                        <  SRR4302533.467868/94‑1 (MQ=60)
                                                          CACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTC                                    <  SRR4302533.5512848/94‑1 (MQ=60)
                                                          CACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGATGCGGACCAGCTGCCCATCACGTCACGTTTACTGTC                                    >  SRR4302533.1926118/1‑94 (MQ=60)
                                                           ACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCC                                   >  SRR4302533.3509999/1‑94 (MQ=60)
                                                           ACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCC                                   <  SRR4302533.4206639/94‑1 (MQ=60)
                                                           ACCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCC                                   <  SRR4302533.5157009/94‑1 (MQ=60)
                                                            CCGACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCG                                  <  SRR4302533.3778277/94‑1 (MQ=60)
                                                              GACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCC                                >  SRR4302533.2161492/1‑94 (MQ=60)
                                                              GACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCC                                <  SRR4302533.859983/94‑1 (MQ=60)
                                                              GACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCC                                >  SRR4302533.4961637/1‑94 (MQ=60)
                                                              GACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCC                                >  SRR4302533.4491878/1‑94 (MQ=60)
                                                              GACGGCATTTTTAATCCCGGCCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCC                                >  SRR4302533.1730340/1‑94 (MQ=60)
                                                               ACGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTTCTGTCTGCCA                               >  SRR4302533.1164350/1‑94 (MQ=60)
                                                                CGGCATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAG                              >  SRR4302533.1393492/1‑94 (MQ=60)
                                                                   CATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGG                           <  SRR4302533.4357635/94‑1 (MQ=60)
                                                                    ATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGC                          >  SRR4302533.3244713/1‑94 (MQ=60)
                                                                    ATTTTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGC                          >  SRR4302533.1954757/1‑94 (MQ=60)
                                                                    ATTTTTAATCCCGGTCAGTAC‑GTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCC                         >  SRR4302533.5785513/1‑94 (MQ=60)
                                                                       TTTAATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCA                       >  SRR4302533.3534132/1‑94 (MQ=60)
                                                                           ATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCA                   >  SRR4302533.2085953/1‑94 (MQ=60)
                                                                           ATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCA                   >  SRR4302533.3624378/1‑94 (MQ=60)
                                                                           ATCCCGGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCA                   <  SRR4302533.5431751/94‑1 (MQ=60)
                                                                              CCGGTCAGTACGGTCGCGGATTGATCGGCAACACGGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCG                <  SRR4302533.3467421/94‑1 (MQ=60)
                                                                                GGTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCA              >  SRR4302533.5505912/1‑94 (MQ=60)
                                                                                 GTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAA             >  SRR4302533.4831801/1‑94 (MQ=60)
                                                                                 GTCAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAA             >  SRR4302533.5159569/1‑94 (MQ=60)
                                                                                   CAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATG           >  SRR4302533.1249348/1‑94 (MQ=60)
                                                                                   CAGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATG           <  SRR4302533.820294/94‑1 (MQ=60)
                                                                                    AGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGG          <  SRR4302533.5711273/94‑1 (MQ=60)
                                                                                    AGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGG          >  SRR4302533.5419032/1‑94 (MQ=60)
                                                                                    AGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGG          >  SRR4302533.2533108/1‑94 (MQ=60)
                                                                                    AGTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGG          <  SRR4302533.3510454/94‑1 (MQ=60)
                                                                                     GTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGT         <  SRR4302533.1118170/94‑1 (MQ=60)
                                                                                     GTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGT         <  SRR4302533.6224027/94‑1 (MQ=60)
                                                                                     GTACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGG         >  SRR4302533.5144067/1‑94 (MQ=60)
                                                                                      TACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTG        <  SRR4302533.5307327/94‑1 (MQ=60)
                                                                                      TACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTG        <  SRR4302533.1253773/94‑1 (MQ=60)
                                                                                       ACGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGG       <  SRR4302533.2477581/94‑1 (MQ=60)
                                                                                        CGGTCGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGC      >  SRR4302533.2337727/1‑94 (MQ=60)
                                                                                            CGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGAT  <  SRR4302533.4393257/94‑1 (MQ=60)
                                                                                            CGCGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGAT  >  SRR4302533.3868242/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATAATGCCTTTGTCACTGGTAACGTTCGCCCCTTTGGCATACAGCACTTTACCGTTTTCACCGACGGCATTTTTAATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGAT  >  NC_000913/2218598‑2218783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑