Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,276,343 T→C intergenic (‑86/+247) yejG ← / ← bcr hypothetical protein/bicyclomycin resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,276,3430TC100.0% 766.6 / NA 234intergenic (‑86/+247)yejG/bcrhypothetical protein/bicyclomycin resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (122/112);  total (122/112)

Only 100 of 239 total aligned reads displayed.

GTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACC  >  NC_000913/2276253‑2276432
                                                                                          |                                                                                         
gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCaa                                                                                         <  2:4376021/93‑1 (MQ=255)
 tCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCaaa                                                                                        >  2:1257303/1‑93 (MQ=255)
   aCTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGa                                                                                      >  1:344073/1‑93 (MQ=255)
    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCNNCTTTATACAGATCGNAAANAt                                                                                     >  1:2807903/1‑93 (MQ=255)
    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGAt                                                                                     <  2:1800670/93‑1 (MQ=255)
      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTg                                                                                   >  2:1051436/1‑93 (MQ=255)
      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTg                                                                                   >  2:2077936/1‑93 (MQ=255)
       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGa                                                                                  <  1:4989236/93‑1 (MQ=255)
       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGa                                                                                  >  1:1299193/1‑93 (MQ=255)
       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGa                                                                                  <  1:1481521/93‑1 (MQ=255)
       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGa                                                                                  >  1:865437/1‑93 (MQ=255)
        ttttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGAt                                                                                 <  1:4639030/93‑1 (MQ=255)
        ttttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGAt                                                                                 >  1:3242178/1‑93 (MQ=255)
         tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATg                                                                                <  1:4482560/93‑1 (MQ=255)
         tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATg                                                                                >  1:3015154/1‑93 (MQ=255)
             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTc                                                                            <  1:2412085/93‑1 (MQ=255)
             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTc                                                                            >  1:5721941/1‑93 (MQ=255)
               cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGc                                                                          <  1:4729308/93‑1 (MQ=255)
               cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGc                                                                          >  2:3918952/1‑93 (MQ=255)
                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCt                                                                        <  1:6225924/93‑1 (MQ=255)
                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCt                                                                        >  1:4783101/1‑93 (MQ=255)
                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCt                                                                        >  1:3466735/1‑93 (MQ=255)
                     cacaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGt                                                                    >  2:2282568/1‑93 (MQ=255)
                        aTTCACTTCACAATTTGTTCACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGc                                                                 >  2:72566/1‑93 (MQ=255)
                         ttcacttcacAATTTGTTTACATCAATTTTAACACATCATCACCAATCCGTCTTTATACAGATCGCAAAGATTGATTCCTCGCCTGCGTGGcc                                                                <  2:803979/93‑1 (MQ=255)
                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCct                                                               <  1:3870751/93‑1 (MQ=255)
                            acttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGTctct                                                             >  2:5473259/1‑93 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            >  1:3678186/1‑93 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            >  2:1423824/1‑93 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            <  2:6224184/93‑1 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            <  2:4709231/93‑1 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            <  2:4627408/93‑1 (MQ=255)
                             cttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                                            <  2:3575831/93‑1 (MQ=255)
                              ttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTAt                                                           <  2:5541091/93‑1 (MQ=255)
                              ttcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTAt                                                           >  1:4588277/1‑93 (MQ=255)
                               tcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                                          >  1:1008556/1‑93 (MQ=255)
                               tcacAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                                          <  1:3663954/93‑1 (MQ=255)
                                 acaATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                                        >  1:2643399/1‑93 (MQ=255)
                                   aaTTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                                      >  2:5155162/1‑93 (MQ=255)
                                   aaTTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                                      >  2:5831330/1‑93 (MQ=255)
                                    aTTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                                     >  1:704021/1‑93 (MQ=255)
                                     tttGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTg                                                    >  2:4695537/1‑93 (MQ=255)
                                      ttGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGa                                                   >  1:2874541/1‑93 (MQ=255)
                                         tttACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                                                >  1:1868818/1‑93 (MQ=255)
                                            aCATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                                             <  1:633978/93‑1 (MQ=255)
                                              aTCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                                           <  1:1160578/93‑1 (MQ=255)
                                              aTCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                                           >  2:216146/1‑93 (MQ=255)
                                               tCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaa                                          <  2:1240978/93‑1 (MQ=255)
                                               tCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaa                                          <  2:2383281/93‑1 (MQ=255)
                                                cAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                                         >  2:6119045/1‑93 (MQ=255)
                                                 aaTTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAata                                        <  1:2538033/93‑1 (MQ=255)
                                                   ttttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                                      <  1:4271198/93‑1 (MQ=255)
                                                   ttttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                                      <  1:1904296/93‑1 (MQ=255)
                                                    tttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                                     <  1:648974/93‑1 (MQ=255)
                                                    tttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                                     <  1:1645317/93‑1 (MQ=255)
                                                    tttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                                     >  2:4988339/1‑93 (MQ=255)
                                                    tttAACACATCATCAACAATCCGTCTCTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                                     <  2:5092311/93‑1 (MQ=255)
                                                     ttAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                                    <  1:1068285/93‑1 (MQ=255)
                                                      tAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGc                                   <  2:5567487/93‑1 (MQ=255)
                                                       aaCACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                                  <  1:2144274/93‑1 (MQ=255)
                                                       aaCACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg                                  <  1:2127618/93‑1 (MQ=255)
                                                         cacaTCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg                                >  1:5177639/1‑93 (MQ=255)
                                                           catcatCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCg                              <  1:908106/93‑1 (MQ=255)
                                                           catcatCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCg                              >  1:173087/1‑93 (MQ=255)
                                                            atcatcAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt                             <  1:406404/93‑1 (MQ=255)
                                                            atcatcAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt                             <  1:3984452/93‑1 (MQ=255)
                                                            atcatcAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt                             <  2:4034349/93‑1 (MQ=255)
                                                             tcatcaACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg                            <  2:1302852/93‑1 (MQ=255)
                                                             tcatcaACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg                            <  1:789691/93‑1 (MQ=255)
                                                               atcaACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGt                          >  1:3906540/1‑93 (MQ=255)
                                                                 caacaaTCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCa                        <  1:1934468/93‑1 (MQ=255)
                                                                    caaTCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGt                     <  1:61531/93‑1 (MQ=255)
                                                                    caaGCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTGTGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGt                     <  1:3120548/93‑1 (MQ=255)
                                                                     aaTCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt                    <  2:2778687/93‑1 (MQ=255)
                                                                     aaTCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt                    >  1:5887025/1‑93 (MQ=255)
                                                                     aaTCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt                    <  2:379965/93‑1 (MQ=255)
                                                                       tCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTg                  <  2:1028961/93‑1 (MQ=255)
                                                                       tCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTg                  >  2:1611603/1‑93 (MQ=255)
                                                                          gTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCgg               >  1:5412036/1‑93 (MQ=255)
                                                                          gTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCgg               >  2:5174816/1‑93 (MQ=255)
                                                                           tCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCggg              <  1:1547995/93‑1 (MQ=255)
                                                                             tttATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGc            <  2:2849728/93‑1 (MQ=255)
                                                                               tataCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCta          <  2:1169834/93‑1 (MQ=255)
                                                                               tataCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCta          <  1:4098237/93‑1 (MQ=255)
                                                                                ataCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCtat         <  2:4145418/93‑1 (MQ=255)
                                                                                 taCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCtata        <  1:4762287/93‑1 (MQ=255)
                                                                                 taCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCtata        <  2:219728/93‑1 (MQ=255)
                                                                                 taCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCtata        <  2:5302355/93‑1 (MQ=255)
                                                                                 taCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCtata        >  2:5337105/1‑93 (MQ=255)
                                                                                  aCAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTatat       >  2:3054154/1‑93 (MQ=255)
                                                                                   cAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATg      <  1:5219630/93‑1 (MQ=255)
                                                                                     gATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTa    >  2:1722163/1‑93 (MQ=255)
                                                                                      aTCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAc   >  2:413435/1‑93 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  >  2:3865018/1‑93 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  <  2:5716896/93‑1 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  >  2:3465419/1‑93 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  <  2:5992485/93‑1 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  >  2:2640660/1‑93 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  <  1:4316526/93‑1 (MQ=255)
                                                                                       tCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGCAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAcc  <  1:3390000/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACC  >  NC_000913/2276253‑2276432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCC  >  NC_000913/2276250‑2276433
                                                                                             |                                                                                          
GAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGC                                                                                            >  SRR4302533.1092380/1‑94 (MQ=60)
 AAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCA                                                                                           >  SRR4302533.6289560/1‑94 (MQ=60)
  AGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAA                                                                                          <  SRR4302533.4887092/94‑1 (MQ=60)
  AGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAA                                                                                          <  SRR4302533.2409171/94‑1 (MQ=60)
    TCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAG                                                                                        <  SRR4302533.3527872/94‑1 (MQ=60)
    TCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAG                                                                                        <  SRR4302533.701099/94‑1 (MQ=60)
     CACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTAGATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGA                                                                                       >  SRR4302533.3396002/1‑94 (MQ=60)
     CACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGA                                                                                       >  SRR4302533.346176/1‑94 (MQ=60)
      ACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCNNCTTTATACAGATCGNAAANAT                                                                                      >  SRR4302533.2825184/1‑94 (MQ=60)
       CTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTTACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATT                                                                                     >  SRR4302533.4655473/1‑94 (MQ=60)
       CTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATT                                                                                     >  SRR4302533.1944948/1‑94 (MQ=60)
       CTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATT                                                                                     >  SRR4302533.4747373/1‑94 (MQ=60)
         TATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGA                                                                                   >  SRR4302533.870505/1‑94 (MQ=60)
         TATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGA                                                                                   <  SRR4302533.2728333/94‑1 (MQ=60)
         TATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGA                                                                                   >  SRR4302533.1307011/1‑94 (MQ=60)
          ATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGAT                                                                                  >  SRR4302533.3262159/1‑94 (MQ=60)
          ATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGAT                                                                                  <  SRR4302533.1490465/94‑1 (MQ=60)
          ATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGAT                                                                                  <  SRR4302533.5019484/94‑1 (MQ=60)
           TTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATG                                                                                 >  SRR4302533.1643694/1‑94 (MQ=60)
           TTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATG                                                                                 <  SRR4302533.4667140/94‑1 (MQ=60)
           TTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATG                                                                                 >  SRR4302533.3033595/1‑94 (MQ=60)
            TTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGC                                                                                <  SRR4302533.5777869/94‑1 (MQ=60)
            TTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGC                                                                                <  SRR4302533.4509705/94‑1 (MQ=60)
             TTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCC                                                                               >  SRR4302533.6308830/1‑94 (MQ=60)
              TCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCT                                                                              >  SRR4302533.3914620/1‑94 (MQ=60)
               CCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTC                                                                             <  SRR4302533.4181268/94‑1 (MQ=60)
               CCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTC                                                                             >  SRR4302533.5756490/1‑94 (MQ=60)
               CCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTC                                                                             >  SRR4302533.1767170/1‑94 (MQ=60)
                CCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCG                                                                            <  SRR4302533.2426984/94‑1 (MQ=60)
                CCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCG                                                                            >  SRR4302533.2577926/1‑94 (MQ=60)
                  CGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCC                                                                          >  SRR4302533.4120633/1‑94 (MQ=60)
                  CGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCC                                                                          <  SRR4302533.4757978/94‑1 (MQ=60)
                   GGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCT                                                                         >  SRR4302533.3488130/1‑94 (MQ=60)
                   GGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCT                                                                         >  SRR4302533.911028/1‑94 (MQ=60)
                   GGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCT                                                                         <  SRR4302533.2490342/94‑1 (MQ=60)
                   GGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCT                                                                         >  SRR4302533.4812078/1‑94 (MQ=60)
                    GAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTG                                                                        <  SRR4302533.6263226/94‑1 (MQ=60)
                     AAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGC                                                                       >  SRR4302533.2994520/1‑94 (MQ=60)
                        CACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTG                                                                    <  SRR4302533.1562726/94‑1 (MQ=60)
                          CATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGC                                                                  >  SRR4302533.2831977/1‑94 (MQ=60)
                           ATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCC                                                                 <  SRR4302533.4049612/94‑1 (MQ=60)
                            TTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCT                                                                <  SRR4302533.3978575/94‑1 (MQ=60)
                            TTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATAGCAAAGATTGATGCCTCGCCTGCGTGGCCT                                                                >  SRR4302533.2084384/1‑94 (MQ=60)
                             TCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTC                                                               <  SRR4302533.3894378/94‑1 (MQ=60)
                             TCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTC                                                               >  SRR4302533.4052479/1‑94 (MQ=60)
                             TCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTC                                                               <  SRR4302533.5627048/94‑1 (MQ=60)
                              CACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCT                                                              >  SRR4302533.532937/1‑94 (MQ=60)
                               ACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTA                                                             >  SRR4302533.3700795/1‑94 (MQ=60)
                                CTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTAT                                                            >  SRR4302533.4616069/1‑94 (MQ=60)
                                 TTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATG                                                           >  SRR4302533.5422832/1‑94 (MQ=60)
                                 TTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATG                                                           >  SRR4302533.1014455/1‑94 (MQ=60)
                                  TCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGG                                                          <  SRR4302533.3873063/94‑1 (MQ=60)
                                  TCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGG                                                          <  SRR4302533.3686477/94‑1 (MQ=60)
                                   CACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGC                                                         >  SRR4302533.2659643/1‑94 (MQ=60)
                                   CACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGC                                                         >  SRR4302533.888679/1‑94 (MQ=60)
                                     CAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTC                                                       >  SRR4302533.2713041/1‑94 (MQ=60)
                                     CAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTC                                                       <  SRR4302533.1709205/94‑1 (MQ=60)
                                      AATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCT                                                      >  SRR4302533.708181/1‑94 (MQ=60)
                                       ATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTG                                                     <  SRR4302533.1091093/94‑1 (MQ=60)
                                       ATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTG                                                     >  SRR4302533.3691451/1‑94 (MQ=60)
                                        TTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGA                                                    >  SRR4302533.2892226/1‑94 (MQ=60)
                                           GTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTT                                                 >  SRR4302533.3168731/1‑94 (MQ=60)
                                           GTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTT                                                 >  SRR4302533.1880212/1‑94 (MQ=60)
                                               ACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGT                                             <  SRR4302533.637708/94‑1 (MQ=60)
                                               ACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGT                                             >  SRR4302533.1333/1‑94 (MQ=60)
                                               ACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGT                                             >  SRR4302533.4740700/1‑94 (MQ=60)
                                               ACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGT                                             >  SRR4302533.1765379/1‑94 (MQ=60)
                                                 ATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAA                                           <  SRR4302533.1167543/94‑1 (MQ=60)
                                                 ATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAA                                           >  SRR4302533.4763558/1‑94 (MQ=60)
                                                 ATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAA                                           >  SRR4302533.223267/1‑94 (MQ=60)
                                                  TCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAAT                                          >  SRR4302533.348620/1‑94 (MQ=60)
                                                  TCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAAT                                          <  SRR4302533.5019859/94‑1 (MQ=60)
                                                    AATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATAT                                        <  SRR4302533.2553658/94‑1 (MQ=60)
                                                     ATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTCATATC                                       >  SRR4302533.2148507/1‑94 (MQ=60)
                                                     ATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATC                                       >  SRR4302533.852775/1‑94 (MQ=60)
                                                      TTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCT                                      <  SRR4302533.1915916/94‑1 (MQ=60)
                                                      TTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCT                                      <  SRR4302533.524485/94‑1 (MQ=60)
                                                      TTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCT                                      <  SRR4302533.4296905/94‑1 (MQ=60)
                                                      TTTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAAATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCT                                      <  SRR4302533.4515025/94‑1 (MQ=60)
                                                       TTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTG                                     <  SRR4302533.652801/94‑1 (MQ=60)
                                                       TTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTG                                     <  SRR4302533.4928048/94‑1 (MQ=60)
                                                       TTTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTG                                     <  SRR4302533.1655279/94‑1 (MQ=60)
                                                        TTAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGC                                    <  SRR4302533.1074571/94‑1 (MQ=60)
                                                         TAACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCG                                   <  SRR4302533.64131/94‑1 (MQ=60)
                                                          AACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  >  SRR4302533.757064/1‑94 (MQ=60)
                                                          AACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  <  SRR4302533.4208238/94‑1 (MQ=60)
                                                          AACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  <  SRR4302533.2157483/94‑1 (MQ=60)
                                                          AACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  <  SRR4302533.2140738/94‑1 (MQ=60)
                                                          AACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  <  SRR4302533.620757/94‑1 (MQ=60)
                                                          AACACATAATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGG                                  <  SRR4302533.3577557/94‑1 (MQ=60)
                                                           ACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGG                                 <  SRR4302533.3133765/94‑1 (MQ=60)
                                                           ACACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGG                                 >  SRR4302533.5208909/1‑94 (MQ=60)
                                                            CACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGC                                <  SRR4302533.3420702/94‑1 (MQ=60)
                                                             ACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCG                               >  SRR4302533.174179/1‑94 (MQ=60)
                                                             ACATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGATCTGATTTAAGTAATATCTGCGGGCG                               >  SRR4302533.1974623/1‑94 (MQ=60)
                                                              CATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGT                              <  SRR4302533.913432/94‑1 (MQ=60)
                                                               ATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTG                             <  SRR4302533.4008714/94‑1 (MQ=60)
                                                               ATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTG                             >  SRR4302533.6337052/1‑94 (MQ=60)
                                                               ATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTG                             <  SRR4302533.408845/94‑1 (MQ=60)
                                                               ATCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTG                             <  SRR4302533.3277159/94‑1 (MQ=60)
                                                                TCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGG                            <  SRR4302533.794338/94‑1 (MQ=60)
                                                                TCATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGG                            <  SRR4302533.2335898/94‑1 (MQ=60)
                                                                 CATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGT                           >  SRR4302533.4908348/1‑94 (MQ=60)
                                                                 CATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGT                           >  SRR4302533.3930403/1‑94 (MQ=60)
                                                                  ATCAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTC                          >  SRR4302533.1300807/1‑94 (MQ=60)
                                                                    CAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAC                        >  SRR4302533.2926285/1‑94 (MQ=60)
                                                                    CAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAC                        >  SRR4302533.5765975/1‑94 (MQ=60)
                                                                    CAACAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAC                        <  SRR4302533.1946270/94‑1 (MQ=60)
                                                                       CAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT                     >  SRR4302533.5922594/1‑94 (MQ=60)
                                                                       CAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT                     <  SRR4302533.61935/94‑1 (MQ=60)
                                                                       CAATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT                     <  SRR4302533.1197704/94‑1 (MQ=60)
                                                                       CAAGCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTGTGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT                     <  SRR4302533.3139738/94‑1 (MQ=60)
                                                                         ATCCGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCGTCTATGGCTCTGATTTAAGTAATATCTGCGGGTGTGGTCACGTTTG                   >  SRR4302533.4805842/1‑94 (MQ=60)
                                                                            CGTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGG                >  SRR4302533.5444579/1‑94 (MQ=60)
                                                                             GTCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGG               <  SRR4302533.3747851/94‑1 (MQ=60)
                                                                              TCTTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGG              <  SRR4302533.1557322/94‑1 (MQ=60)
                                                                                TTTATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCT            >  SRR4302533.4363444/1‑94 (MQ=60)
                                                                                  TATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTAT          <  SRR4302533.4123183/94‑1 (MQ=60)
                                                                                   ATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTTACGTTTGCGGGGCTttt         >  SRR4302533.1494281/1‑91 (MQ=60)
                                                                                   ATACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATA         >  SRR4302533.5944983/1‑94 (MQ=60)
                                                                                    TACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATAT        <  SRR4302533.4791172/94‑1 (MQ=60)
                                                                                     ACAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATG       <  SRR4302533.4533659/94‑1 (MQ=60)
                                                                                      CAGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGT      <  SRR4302533.5251045/94‑1 (MQ=60)
                                                                                       AGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTA     <  SRR4302533.4331116/94‑1 (MQ=60)
                                                                                       AGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTA     <  SRR4302533.1892759/94‑1 (MQ=60)
                                                                                       AGATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTA     >  SRR4302533.1015281/1‑94 (MQ=60)
                                                                                        GATCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTAC    <  SRR4302533.683700/94‑1 (MQ=60)
                                                                                          TCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCC  <  SRR4302533.4342519/94‑1 (MQ=60)
                                                                                          TCGCAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGCAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCC  <  SRR4302533.3410935/94‑1 (MQ=60)
                                                                                             |                                                                                          
GAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTTTGCGGGGCTATATGTACCC  >  NC_000913/2276250‑2276433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑