Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,375,891 T→C V463V (GTA→GTG)  menD ← 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,8910TC100.0% 753.1 / NA 230V463V (GTA→GTG) menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (112/118);  total (112/118)

Only 100 of 246 total aligned reads displayed.

TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT  >  NC_000913/2375801‑2375980
                                                                                          |                                                                                         
 tGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAcc                                                                                        >  1:256528/1‑93 (MQ=255)
 tGCGACATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAcc                                                                                        <  1:5360890/93‑1 (MQ=255)
  gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCa                                                                                       <  1:3657888/93‑1 (MQ=255)
      cATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcgg                                                                                   >  1:2961569/1‑93 (MQ=255)
       aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggc                                                                                  >  2:1045817/1‑93 (MQ=255)
        tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggcg                                                                                 >  1:186829/1‑93 (MQ=255)
        tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggcg                                                                                 <  2:566263/93‑1 (MQ=255)
         cAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGgcgc                                                                                <  1:5206852/93‑1 (MQ=255)
           gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACAATTAACACCAGCGGCGCAg                                                                              >  1:2646138/1‑93 (MQ=255)
            aTAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGa                                                                             >  1:6082766/1‑93 (MQ=255)
            aTAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGa                                                                             <  2:3210669/93‑1 (MQ=255)
             tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaa                                                                            >  1:1011086/1‑93 (MQ=255)
              aGAACCGCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaaa                                                                           <  2:5319053/93‑1 (MQ=255)
              aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaaa                                                                           >  1:772071/1‑93 (MQ=255)
                 aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCt                                                                        >  1:2702042/1‑93 (MQ=255)
                 aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCt                                                                        <  1:5134490/93‑1 (MQ=255)
                  acgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTg                                                                       >  2:2378495/1‑93 (MQ=255)
                   cgctcacgctcGCTTTGCGGCGTTGTCAGCAGCGAGAAATTTTCCCCGCCGCGGTTGTTCACCACAATTACCACCAGCGGCGCAGAAACCTGa                                                                      <  2:1979175/93‑1 (MQ=255)
                   cgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGa                                                                      <  2:790419/93‑1 (MQ=255)
                    cctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAc                                                                     <  1:4549016/92‑1 (MQ=255)
                      tcacgctcGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGc                                                                   <  2:2288170/93‑1 (MQ=255)
                        acgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCaa                                                                 >  2:4924007/1‑93 (MQ=255)
                           ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATaa                                                              >  2:2474489/1‑93 (MQ=255)
                            tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAc                                                             >  1:3622118/1‑93 (MQ=255)
                             cgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACg                                                            <  2:3361947/93‑1 (MQ=255)
                              gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAACGGCGCAGAAAACTGACGCCATAAAGc                                                           >  1:4302427/1‑93 (MQ=255)
                               ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                                          >  1:3070515/1‑93 (MQ=255)
                                 ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                                        <  2:3062212/93‑1 (MQ=255)
                                 ttGCGCCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                                        <  2:1838466/93‑1 (MQ=255)
                                    cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                                     >  1:3395105/1‑93 (MQ=255)
                                    cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                                     <  2:647292/93‑1 (MQ=255)
                                     ggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCCCAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcccgcccg                                                    >  2:4356700/1‑93 (MQ=255)
                                       cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                                                  <  1:5807051/93‑1 (MQ=255)
                                       cgTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                                                  <  2:4556163/93‑1 (MQ=255)
                                        gTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACCAATAAAACCCACGGGGCCCAAAACCGACGGAAAAAAGCCAGCGCGGTg                                                 >  2:4324129/1‑93 (MQ=255)
                                         ttGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                                                >  1:547625/1‑93 (MQ=255)
                                           ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                                              <  1:2928968/93‑1 (MQ=255)
                                           ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                                              >  2:5835787/1‑93 (MQ=255)
                                            gCACCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                                             <  2:2831371/93‑1 (MQ=255)
                                            gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                                             >  2:4434526/1‑93 (MQ=255)
                                            gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGAGTTGAGAt                                             <  2:6149132/93‑1 (MQ=255)
                                             cAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAta                                            >  2:4659298/1‑92 (MQ=255)
                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          >  2:6245859/1‑93 (MQ=255)
                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          <  2:1759570/93‑1 (MQ=255)
                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          >  1:2157600/1‑93 (MQ=255)
                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:1645295/93‑1 (MQ=255)
                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:4125707/93‑1 (MQ=255)
                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:1824400/93‑1 (MQ=255)
                                                cAGAGAGAAACTTGCCCCGCCGCTTTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  2:2188233/93‑1 (MQ=255)
                                                aaGCGAAAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCGATAACGCCAGCGCGTTGAGATCGTa                                         <  1:4482038/92‑1 (MQ=255)
                                                  gcaagaAATTTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  2:37773/90‑1 (MQ=255)
                                                  gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  2:248614/93‑1 (MQ=255)
                                                  gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  1:4151838/93‑1 (MQ=255)
                                                   cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                                      >  2:3848950/1‑93 (MQ=255)
                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  2:6104955/93‑1 (MQ=255)
                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  2:2164739/93‑1 (MQ=255)
                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  1:4611565/93‑1 (MQ=255)
                                                     agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                                    >  2:5090176/1‑93 (MQ=255)
                                                      gaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   >  1:5638755/1‑93 (MQ=255)
                                                      gaAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   <  2:2882622/93‑1 (MQ=255)
                                                      aaaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   <  2:5799185/92‑1 (MQ=255)
                                                       aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg                                  <  2:3994520/93‑1 (MQ=255)
                                                       aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg                                  >  1:5502529/1‑93 (MQ=255)
                                                        aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg                                 <  1:5024795/93‑1 (MQ=255)
                                                           tttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga                              <  2:1804732/93‑1 (MQ=255)
                                                            ttGCCCGTCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag                             >  2:5986631/1‑93 (MQ=255)
                                                             tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga                            <  2:4135855/93‑1 (MQ=255)
                                                             tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga                            <  1:1139591/93‑1 (MQ=255)
                                                              gCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGACACCTGACGCAATAACGCCAGCGCGTTGAGCTCGTAAAGTGCGgagagag                           >  2:1540953/1‑92 (MQ=255)
                                                              gCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt                           <  1:5942407/93‑1 (MQ=255)
                                                               cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc                          >  1:3563773/1‑93 (MQ=255)
                                                               cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc                          <  1:4102725/93‑1 (MQ=255)
                                                                   ccgCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc                      <  2:765485/93‑1 (MQ=255)
                                                                    cgctgttgttCACCTCCGTACACACCAGCGGAGCAGCAACCTGACGCAGAAACGCCAGCGCGTTGGGATCGTACAGTGCGGAGAGATCGCCca                     <  1:6310018/90‑1 (MQ=255)
                                                                    cgctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCca                     >  1:3828560/4‑93 (MQ=255)
                                                                    cgctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCca                     >  2:190005/4‑93 (MQ=255)
                                                                     gctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCcac                    >  1:231787/3‑93 (MQ=255)
                                                                       tgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACaa                  >  2:2622233/1‑93 (MQ=255)
                                                                        gtttttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 <  1:1520934/93‑1 (MQ=255)
                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAACCCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 <  2:4799608/93‑1 (MQ=255)
                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 >  2:4047419/1‑93 (MQ=255)
                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 >  2:1862187/1‑93 (MQ=255)
                                                                         ttgttCACCACAATTACCACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  2:1640644/93‑1 (MQ=255)
                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGCTCGCCCACCATc                >  1:2729456/1‑93 (MQ=255)
                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  1:396974/93‑1 (MQ=255)
                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  1:330677/93‑1 (MQ=255)
                                                                           gttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc              <  2:269681/93‑1 (MQ=255)
                                                                           gttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc              <  2:4483371/93‑1 (MQ=255)
                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGcc             >  2:2063024/1‑93 (MQ=255)
                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGcc             >  1:3162282/1‑93 (MQ=255)
                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGAAAAGTGCGGAGAGATCGCCCACAATCGcc             <  1:2594971/93‑1 (MQ=255)
                                                                             tCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCa            >  1:3731649/1‑93 (MQ=255)
                                                                                 cacaATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGt        >  2:4403824/1‑93 (MQ=255)
                                                                                  acaATTAACGCCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCTCCAGCGTc       <  2:549125/93‑1 (MQ=255)
                                                                                  acaATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTc       >  2:2524475/1‑93 (MQ=255)
                                                                                   caATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCg      <  2:3606007/93‑1 (MQ=255)
                                                                                      ttAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  2:5932546/93‑1 (MQ=255)
                                                                                      ttAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  2:1238597/93‑1 (MQ=255)
                                                                                      atAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  1:136632/92‑1 (MQ=255)
                                                                                       tAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGttt  >  1:4768808/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT  >  NC_000913/2375801‑2375980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCC  >  NC_000913/2375798‑2375983
                                                                                             |                                                                                            
GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTACCCGCCGCTGTTGTTCACCACAATTAAC                                                                                              >  SRR4302533.6097319/1‑94 (MQ=60)
CTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAAC                                                                                              <  SRR4302533.5314482/94‑1 (MQ=60)
  TTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAC                                                                                            <  SRR4302533.5280630/94‑1 (MQ=60)
   TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACC                                                                                           >  SRR4302533.258093/1‑94 (MQ=60)
    TGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                                          <  SRR4302533.2735769/94‑1 (MQ=60)
    TGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                                          <  SRR4302533.5890508/94‑1 (MQ=60)
    TGCGACATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCA                                                                                          <  SRR4302533.5393088/94‑1 (MQ=60)
     GCGGCATCAGATAGAAACGCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                                         <  SRR4302533.4606839/94‑1 (MQ=60)
     GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                                         <  SRR4302533.3680379/94‑1 (MQ=60)
     GCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAG                                                                                         <  SRR4302533.3680380/94‑1 (MQ=60)
      CGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGC                                                                                        <  SRR4302533.4211501/94‑1 (MQ=60)
       GGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCG                                                                                       <  SRR4302533.1962124/94‑1 (MQ=60)
        GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                                      >  SRR4302533.4137132/1‑94 (MQ=60)
        GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                                      >  SRR4302533.2979672/1‑94 (MQ=60)
        GCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGG                                                                                      >  SRR4302533.2514832/1‑94 (MQ=60)
         CATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGC                                                                                     >  SRR4302533.2597472/1‑94 (MQ=60)
          ATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCG                                                                                    >  SRR4302533.187991/1‑94 (MQ=60)
          ATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCG                                                                                    <  SRR4302533.3225839/94‑1 (MQ=60)
            CAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCA                                                                                  <  SRR4302533.5238223/94‑1 (MQ=60)
             AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACAATTAACACCAGCGGCGCAG                                                                                 >  SRR4302533.2662398/1‑94 (MQ=60)
             AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                                                 >  SRR4302533.4510519/1‑94 (MQ=60)
             AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                                                 >  SRR4302533.5174942/1‑94 (MQ=60)
             AGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAG                                                                                 >  SRR4302533.872714/1‑94 (MQ=60)
              GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGA                                                                                <  SRR4302533.4289061/94‑1 (MQ=60)
              GATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGA                                                                                >  SRR4302533.6119545/1‑94 (MQ=60)
               ATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAA                                                                               >  SRR4302533.1017002/1‑94 (MQ=60)
                TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAA                                                                              <  SRR4302533.4131548/94‑1 (MQ=60)
                TAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAA                                                                              >  SRR4302533.776614/1‑94 (MQ=60)
                  GAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACC                                                                            <  SRR4302533.2994526/94‑1 (MQ=60)
                   AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCT                                                                           >  SRR4302533.2718617/1‑94 (MQ=60)
                   AAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCT                                                                           <  SRR4302533.2276468/94‑1 (MQ=60)
                    AACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTG                                                                          <  SRR4302533.5165512/94‑1 (MQ=60)
                      CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAC                                                                        >  SRR4302533.3186494/1‑94 (MQ=60)
                      CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAC                                                                        >  SRR4302533.3021115/1‑94 (MQ=60)
                      CGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGAC                                                                        >  SRR4302533.333220/1‑94 (MQ=60)
                       GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCTCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                                       >  SRR4302533.4114019/1‑94 (MQ=60)
                       GCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                                       <  SRR4302533.5693900/94‑1 (MQ=60)
                       CCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACG                                                                       <  SRR4302533.4576601/94‑1 (MQ=60)
                        CTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGC                                                                      >  SRR4302533.6256442/1‑94 (MQ=60)
                          CACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAA                                                                    >  SRR4302533.451790/1‑94 (MQ=60)
                          CACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAA                                                                    >  SRR4302533.3668311/1‑94 (MQ=60)
                              CTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAC                                                                >  SRR4302533.3644407/1‑94 (MQ=60)
                               TCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACG                                                               <  SRR4302533.5521699/94‑1 (MQ=60)
                                CGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGC                                                              <  SRR4302533.5670353/94‑1 (MQ=60)
                                CGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAACGGCGCAGAAAACTGACGCCATAAAGC                                                              >  SRR4302533.4328310/1‑94 (MQ=60)
                                 GCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGTC                                                             >  SRR4302533.1873707/1‑94 (MQ=60)
                                 GCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCC                                                             >  SRR4302533.3089368/1‑94 (MQ=60)
                                  CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                                            >  SRR4302533.2533207/1‑94 (MQ=60)
                                  CTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAAGCCA                                                            >  SRR4302533.1323598/1‑94 (MQ=60)
                                  CTTTGCGGCGTTGGCAACAGCGAGAAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCA                                                            <  SRR4302533.2778501/94‑1 (MQ=60)
                                     TGCGGCGTTGGCAACAGCGATAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCG                                                         <  SRR4302533.4944606/94‑1 (MQ=60)
                                      GCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGC                                                        >  SRR4302533.3416073/1‑94 (MQ=60)
                                       CGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCG                                                       <  SRR4302533.6262369/94‑1 (MQ=60)
                                       CGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCCGAAGCAATCACGCCAGCGCG                                                       >  SRR4302533.4741605/1‑94 (MQ=60)
                                       CGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCG                                                       <  SRR4302533.5565897/94‑1 (MQ=60)
                                        GGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGT                                                      >  SRR4302533.141555/1‑94 (MQ=60)
                                          CGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTG                                                    <  SRR4302533.5842116/94‑1 (MQ=60)
                                          CGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACCCCAGCGCGTTG                                                    >  SRR4302533.5246071/1‑94 (MQ=60)
                                           GTTGGCAACAGCGAGAGAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                                                   >  SRR4302533.5147996/1‑94 (MQ=60)
                                           GTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA                                                   >  SRR4302533.550876/1‑94 (MQ=60)
                                            TTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                                                  <  SRR4302533.4794625/94‑1 (MQ=60)
                                            GTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAG                                                  <  SRR4302533.3841533/94‑1 (MQ=60)
                                              GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                                                <  SRR4302533.1725112/94‑1 (MQ=60)
                                              GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                                                <  SRR4302533.2946886/94‑1 (MQ=60)
                                              GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                                                <  SRR4302533.5413124/94‑1 (MQ=60)
                                              GGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAT                                                >  SRR4302533.2095891/1‑94 (MQ=60)
                                                 AACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                                             >  SRR4302533.2170882/1‑94 (MQ=60)
                                                 AACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGT                                             <  SRR4302533.2901365/94‑1 (MQ=60)
                                                   CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                                           <  SRR4302533.4150752/94‑1 (MQ=60)
                                                   CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                                           <  SRR4302533.1835545/94‑1 (MQ=60)
                                                   CAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAA                                           <  SRR4302533.1655257/94‑1 (MQ=60)
                                                   AAGCGAAAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCGATAACGCCAGCGCGTTGAGATCGTAA                                           <  SRR4302533.4509171/94‑1 (MQ=60)
                                                    aggaAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAA                                          <  SRR4302533.4364640/90‑1 (MQ=60)
                                                     GCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAG                                         <  SRR4302533.4176994/94‑1 (MQ=60)
                                                      gcAGAAAATTGGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                                        <  SRR4302533.3133453/92‑1 (MQ=60)
                                                      gaaaAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGT                                        <  SRR4302533.3620627/90‑1 (MQ=60)
                                                       GAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTG                                       <  SRR4302533.4639505/94‑1 (MQ=60)
                                                        AGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGC                                      >  SRR4302533.5672845/1‑94 (MQ=60)
                                                         GAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                                     <  SRR4302533.2972983/94‑1 (MQ=60)
                                                         GAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCG                                     >  SRR4302533.5535729/1‑94 (MQ=60)
                                                           AAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGA                                   <  SRR4302533.5055221/94‑1 (MQ=60)
                                                             ATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAACGCGTTGAGATCGTAAAGTGCGGAGC                                 >  SRR4302533.663262/1‑94 (MQ=60)
                                                             ATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAAACTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGG                                 >  SRR4302533.5853805/1‑94 (MQ=60)
                                                              TTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAGGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAG                                <  SRR4302533.5096170/94‑1 (MQ=60)
                                                               TTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGA                               >  SRR4302533.5267634/1‑94 (MQ=60)
                                                                TGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT                              <  SRR4302533.1146373/94‑1 (MQ=60)
                                                                TGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAT                              <  SRR4302533.2703147/94‑1 (MQ=60)
                                                                 GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC                             >  SRR4302533.3585732/1‑94 (MQ=60)
                                                                 GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC                             >  SRR4302533.5982236/1‑94 (MQ=60)
                                                                 GCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATC                             <  SRR4302533.5978344/94‑1 (MQ=60)
                                                                  CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG                            <  SRR4302533.2646872/94‑1 (MQ=60)
                                                                  CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG                            <  SRR4302533.4127687/94‑1 (MQ=60)
                                                                  CCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCG                            >  SRR4302533.1526708/1‑94 (MQ=60)
                                                                   CCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGC                           >  SRR4302533.4174405/1‑94 (MQ=60)
                                                                      CCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCA                        >  SRR4302533.3851969/1‑94 (MQ=60)
                                                                       CGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCAC                       >  SRR4302533.233220/1‑94 (MQ=60)
                                                                          TGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAT                    >  SRR4302533.1576269/1‑94 (MQ=60)
                                                                           GTTTTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATC                   <  SRR4302533.1530091/94‑1 (MQ=60)
                                                                           GTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGCTCGCCCACCATC                   >  SRR4302533.2746198/1‑94 (MQ=60)
                                                                            TTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCG                  <  SRR4302533.399374/94‑1 (MQ=60)
                                                                            TTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCG                  <  SRR4302533.399375/94‑1 (MQ=60)
                                                                            TTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCG                  <  SRR4302533.332699/94‑1 (MQ=60)
                                                                            TTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCG                  >  SRR4302533.827423/1‑94 (MQ=60)
                                                                            TTGTTCACAACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCG                  <  SRR4302533.3485707/94‑1 (MQ=60)
                                                                              GTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCC                >  SRR4302533.3181744/1‑94 (MQ=60)
                                                                               TTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCA               >  SRR4302533.3754538/1‑94 (MQ=60)
                                                                               TTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCA               >  SRR4302533.3457750/1‑94 (MQ=60)
                                                                               TTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGAAAAGTGCGGAGAGATCGCCCACAATCGCCA               <  SRR4302533.2610920/94‑1 (MQ=60)
                                                                                   CCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGT           >  SRR4302533.3693866/1‑94 (MQ=60)
                                                                                    CACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTC          >  SRR4302533.1775287/1‑94 (MQ=60)
                                                                                    CACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTC          >  SRR4302533.2638555/1‑94 (MQ=60)
                                                                                     ACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCG         >  SRR4302533.1118563/1‑94 (MQ=60)
                                                                                         TTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT     >  SRR4302533.4797713/1‑94 (MQ=60)
                                                                                         TTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT     >  SRR4302533.377766/1‑94 (MQ=60)
                                                                                           AACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGC   >  SRR4302533.2964609/1‑94 (MQ=60)
                                                                                           AACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGC   >  SRR4302533.2652060/1‑94 (MQ=60)
                                                                                            ACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCC  <  SRR4302533.5924039/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCC  >  NC_000913/2375798‑2375983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑