Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,438,697 A→C L310R (CTG→CGG)  fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,6970AC100.0% 577.2 / NA 182L310R (CTG→CGG) fabB3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (87/95);  total (87/95)

Only 100 of 186 total aligned reads displayed.

ACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATG  >  NC_000913/2438607‑2438786
                                                                                          |                                                                                         
aCGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGc                                                                                         <  1:5962493/93‑1 (MQ=255)
  gCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGctc                                                                                       <  1:1022171/93‑1 (MQ=255)
   ccAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGctct                                                                                      <  1:496541/93‑1 (MQ=255)
   ccAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGctct                                                                                      <  1:1966702/93‑1 (MQ=255)
    cagcagCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCtt                                                                                     <  1:5135548/93‑1 (MQ=255)
    cagcagCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCtt                                                                                     <  2:4585345/93‑1 (MQ=255)
     agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  2:4696146/93‑1 (MQ=255)
     agcagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCttt                                                                                    <  2:2428980/93‑1 (MQ=255)
       cagcGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCa                                                                                  <  2:5908517/93‑1 (MQ=255)
         gcgcCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACg                                                                                >  2:6156664/1‑93 (MQ=255)
             ccAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGc                                                                            <  2:4636443/93‑1 (MQ=255)
             ccAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGc                                                                            >  1:5668053/1‑93 (MQ=255)
             ccAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGc                                                                            >  2:367058/1‑93 (MQ=255)
              cAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCACGATCTTTCACCTCGcc                                                                           >  2:5503188/1‑93 (MQ=255)
               agagagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCa                                                                          >  1:3263458/1‑93 (MQ=255)
                gagagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCaa                                                                         >  2:3402693/1‑93 (MQ=255)
                 agagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAAc                                                                        <  1:5630294/93‑1 (MQ=255)
                 agagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAAc                                                                        <  1:5141193/93‑1 (MQ=255)
                 agagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAAc                                                                        <  1:3772714/93‑1 (MQ=255)
                  gagTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAAcc                                                                       <  1:2402830/93‑1 (MQ=255)
                   agTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCg                                                                      <  2:844387/93‑1 (MQ=255)
                    gTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCgg                                                                     >  2:2208012/1‑93 (MQ=255)
                      gACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAg                                                                   <  1:6059565/93‑1 (MQ=255)
                        ccGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTc                                                                 <  2:1680262/93‑1 (MQ=255)
                         cGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCg                                                                <  2:4294507/93‑1 (MQ=255)
                          ggTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGa                                                               <  2:4873102/93‑1 (MQ=255)
                           gTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCCCCCACCGGAGTCGag                                                              >  2:1657717/1‑92 (MQ=255)
                             cATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGt                                                            <  2:3847879/93‑1 (MQ=255)
                              aTGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTa                                                           <  2:5328781/93‑1 (MQ=255)
                               tGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAc                                                          >  1:4885595/1‑93 (MQ=255)
                               tGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAc                                                          >  2:3249652/1‑93 (MQ=255)
                                ggCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc                                                         >  2:568509/1‑93 (MQ=255)
                                  cTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCgg                                                       >  2:297840/1‑92 (MQ=255)
                                  cTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGt                                                       <  1:5832087/93‑1 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGGCAACCGGAGTCGAAGGACCGTg                                                      >  2:2995091/1‑93 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGCGCCGAAGTACCGTg                                                      >  1:3048987/1‑93 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      <  2:4993711/93‑1 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      >  2:212535/1‑93 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      >  2:3712616/1‑93 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      >  1:3537341/1‑93 (MQ=255)
                                   tttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTg                                                      >  1:3823319/1‑93 (MQ=255)
                                    ttGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGACGAAGTACCGTgg                                                     >  1:5930553/1‑93 (MQ=255)
                                    ttGGTTGCAGAAATCGCCGGGCTCTTAACGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTgg                                                     <  1:930626/93‑1 (MQ=255)
                                      ggTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGa                                                   <  2:2901766/93‑1 (MQ=255)
                                       gTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAg                                                  >  1:4320130/1‑93 (MQ=255)
                                        ttGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGt                                                 <  2:4223949/93‑1 (MQ=255)
                                        gtGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGt                                                 <  2:332978/92‑1 (MQ=255)
                                          gCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTc                                               >  1:4495870/1‑93 (MQ=255)
                                           cAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGGACCGTGGGAGGTCa                                              >  1:1097212/1‑93 (MQ=255)
                                            aGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAg                                             <  1:2605557/93‑1 (MQ=255)
                                             gAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAgg                                            >  1:3579075/1‑93 (MQ=255)
                                              aaaTCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGt                                           <  2:5545684/93‑1 (MQ=255)
                                               aaTCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTa                                          >  2:5258558/1‑93 (MQ=255)
                                                 tCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAAt                                        <  2:4385572/93‑1 (MQ=255)
                                                 tCGCCGGGCTCTGATCGCCGAACACTTCAGGGATAGCTGCCCGCTCGTTCACGTCGCCAACCGGAGTCAAAGTACCGTGGGAGTTCAGGTAAt                                        <  2:5831230/93‑1 (MQ=255)
                                                 tCGCCCGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAAt                                        <  1:5280067/93‑1 (MQ=255)
                                                   gCCGCGCTCTTATCGCCGAACGCTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCg                                      <  1:795367/93‑1 (MQ=255)
                                                      gggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAtt                                   >  1:1491269/1‑93 (MQ=255)
                                                      gggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAtt                                   >  1:1621067/1‑93 (MQ=255)
                                                      gggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAtt                                   >  2:4119465/1‑93 (MQ=255)
                                                       ggCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGCCGAAGTACCGTCGGAGTTCCGGCAATCGATTg                                  >  1:2410498/1‑93 (MQ=255)
                                                        gCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTgg                                 <  1:3970446/93‑1 (MQ=255)
                                                         ctctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTggg                                <  1:2207319/93‑1 (MQ=255)
                                                          tctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTgggg                               <  2:1552761/93‑1 (MQ=255)
                                                          tctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTgggg                               <  2:5902542/93‑1 (MQ=255)
                                                           ctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTGACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGt                              >  1:980596/1‑93 (MQ=255)
                                                           ctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGt                              >  2:1117004/1‑93 (MQ=255)
                                                           ctTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGt                              <  1:2818903/93‑1 (MQ=255)
                                                            ttATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTa                             <  2:565157/93‑1 (MQ=255)
                                                              aTCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATc                           <  2:6066849/93‑1 (MQ=255)
                                                                cGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCaa                         <  2:5149201/93‑1 (MQ=255)
                                                                 gCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAc                        <  1:1344484/93‑1 (MQ=255)
                                                                  ccGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACg                       <  2:508684/93‑1 (MQ=255)
                                                                   cGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGc                      <  2:376807/93‑1 (MQ=255)
                                                                   cGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGc                      >  2:6107122/1‑93 (MQ=255)
                                                                    gAACACTTCACGGATAGCTGCCCGCTCTTTTACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGcc                     >  2:2599537/1‑93 (MQ=255)
                                                                    gAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGcc                     >  1:1849590/1‑93 (MQ=255)
                                                                    gAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGcc                     <  1:2827994/93‑1 (MQ=255)
                                                                     aaCACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCa                    >  1:1998272/1‑93 (MQ=255)
                                                                      acacTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCAt                   <  2:760773/93‑1 (MQ=255)
                                                                       cacTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATg                  >  1:5519436/1‑93 (MQ=255)
                                                                        ccTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 <  2:1704249/92‑1 (MQ=255)
                                                                        acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 >  2:4151819/1‑93 (MQ=255)
                                                                        acTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGc                 >  1:4567888/1‑93 (MQ=255)
                                                                         cTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCa                >  1:2146460/1‑93 (MQ=255)
                                                                         cTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCa                <  2:2625409/93‑1 (MQ=255)
                                                                         cTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCa                >  2:3879660/1‑93 (MQ=255)
                                                                          ttCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCAt               >  1:680268/1‑93 (MQ=255)
                                                                          ttCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCAt               >  2:1396417/1‑93 (MQ=255)
                                                                            cACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCg             >  2:678472/1‑93 (MQ=255)
                                                                             aCGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGc            <  2:1149682/93‑1 (MQ=255)
                                                                                gATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCAt         >  2:3208574/1‑93 (MQ=255)
                                                                                gATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCAt         <  2:4016787/93‑1 (MQ=255)
                                                                                gATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCAt         <  2:3446479/93‑1 (MQ=255)
                                                                                  tAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGATCAGGTAATCCATTGGGGTATCAACGCCATGCATCGGCctct       >  2:2348950/1‑93 (MQ=255)
                                                                                   aGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCtt      <  1:2021394/93‑1 (MQ=255)
                                                                                   aGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCtt      <  2:1901869/93‑1 (MQ=255)
                                                                                    gCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTc     >  2:4513145/1‑93 (MQ=255)
                                                                                     cTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCa    <  2:5164748/93‑1 (MQ=255)
                                                                                      tGCCCGCTCTTTCACGTCGCCAACAGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCAt   >  2:4606156/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATG  >  NC_000913/2438607‑2438786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGC  >  NC_000913/2438604‑2438790
                                                                                             |                                                                                             
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCC                                                                                               <  SRR4302533.4714891/94‑1 (MQ=60)
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCC                                                                                               >  SRR4302533.1416471/1‑94 (MQ=60)
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCC                                                                                               <  SRR4302533.1431933/94‑1 (MQ=60)
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCC                                                                                               <  SRR4302533.5087629/94‑1 (MQ=60)
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCC                                                                                               <  SRR4302533.5163858/94‑1 (MQ=60)
 GTACGCCAGCAGCGCCTAGGGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCG                                                                                              <  SRR4302533.5225009/94‑1 (MQ=60)
 GTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCG                                                                                              <  SRR4302533.3072927/94‑1 (MQ=60)
 GTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCG                                                                                              >  SRR4302533.1272361/1‑94 (MQ=60)
 GTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCG                                                                                              <  SRR4302533.854694/94‑1 (MQ=60)
   ACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCT                                                                                            <  SRR4302533.4951167/94‑1 (MQ=60)
   ACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCT                                                                                            <  SRR4302533.5998564/94‑1 (MQ=60)
     GCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCT                                                                                          <  SRR4302533.1028178/94‑1 (MQ=60)
     GCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCT                                                                                          >  SRR4302533.4575862/1‑94 (MQ=60)
      CCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTT                                                                                         <  SRR4302533.1978722/94‑1 (MQ=60)
      CCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTT                                                                                         <  SRR4302533.499502/94‑1 (MQ=60)
       CAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTT                                                                                        <  SRR4302533.5166578/94‑1 (MQ=60)
       CAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTT                                                                                        <  SRR4302533.2085328/94‑1 (MQ=60)
           AGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACG                                                                                    <  SRR4302533.5714218/94‑1 (MQ=60)
             CGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTC                                                                                  <  SRR4302533.3822528/94‑1 (MQ=60)
             CGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTC                                                                                  >  SRR4302533.562391/1‑94 (MQ=60)
               CCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGC                                                                                >  SRR4302533.5702291/1‑94 (MQ=60)
                 CAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCA                                                                              >  SRR4302533.3283588/1‑94 (MQ=60)
                 CAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCA                                                                              >  SRR4302533.1606760/1‑94 (MQ=60)
                 CAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCA                                                                              <  SRR4302533.3306396/94‑1 (MQ=60)
                 CAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCA                                                                              >  SRR4302533.4825521/1‑94 (MQ=60)
                    AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACC                                                                           <  SRR4302533.5172263/94‑1 (MQ=60)
                    AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACC                                                                           <  SRR4302533.3795841/94‑1 (MQ=60)
                    AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACC                                                                           <  SRR4302533.5664342/94‑1 (MQ=60)
                    AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACC                                                                           >  SRR4302533.2420251/1‑94 (MQ=60)
                     GAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCG                                                                          <  SRR4302533.2417668/94‑1 (MQ=60)
                        TGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAG                                                                       <  SRR4302533.5000314/94‑1 (MQ=60)
                         GACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGT                                                                      <  SRR4302533.6096247/94‑1 (MQ=60)
                              GTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAG                                                                 <  SRR4302533.2710647/94‑1 (MQ=60)
                                 ATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAC                                                              >  SRR4302533.4915193/1‑94 (MQ=60)
                                     CTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGCGCCGAAGTACCGTG                                                          >  SRR4302533.3067651/1‑94 (MQ=60)
                                     CTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTG                                                          <  SRR4302533.5867335/94‑1 (MQ=60)
                                     CTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTG                                                          >  SRR4302533.3846696/1‑94 (MQ=60)
                                     CTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTG                                                          >  SRR4302533.3559142/1‑94 (MQ=60)
                                      TTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGACGAAGTACCGTGG                                                         >  SRR4302533.5966405/1‑94 (MQ=60)
                                       TTGGTTGCAGAAATCGCCGGGCTCTTAACGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGG                                                        <  SRR4302533.936088/94‑1 (MQ=60)
                                        TGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGA                                                       >  SRR4302533.886540/1‑94 (MQ=60)
                                         GGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAG                                                      >  SRR4302533.4346149/1‑94 (MQ=60)
                                         GGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAG                                                      >  SRR4302533.253654/1‑94 (MQ=60)
                                         GGGTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGCTAGCTGCCCGCTCTTTTACGTCGCCCACCGGAGGCGAAGTACCGGGGGGG                                                      >  SRR4302533.4469345/1‑94 (MQ=60)
                                            TGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTC                                                   >  SRR4302533.4523094/1‑94 (MQ=60)
                                             GCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGGACCGTGGGAGGTCA                                                  >  SRR4302533.1103709/1‑94 (MQ=60)
                                              CAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAG                                                 <  SRR4302533.200876/94‑1 (MQ=60)
                                               AGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGG                                                >  SRR4302533.3601127/1‑94 (MQ=60)
                                               AGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGG                                                <  SRR4302533.2621562/94‑1 (MQ=60)
                                                GAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGT                                               <  SRR4302533.2333674/94‑1 (MQ=60)
                                                    TCGCCCGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATC                                           <  SRR4302533.5311709/94‑1 (MQ=60)
                                                      GCCGCGCTCTTATCGCCGAACGCTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGA                                         <  SRR4302533.800046/94‑1 (MQ=60)
                                                       CCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAT                                        >  SRR4302533.5943200/1‑94 (MQ=60)
                                                       CCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGAT                                        <  SRR4302533.720065/94‑1 (MQ=60)
                                                        CGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATT                                       >  SRR4302533.1630889/1‑94 (MQ=60)
                                                        CGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATT                                       >  SRR4302533.1500262/1‑94 (MQ=60)
                                                        CGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATT                                       >  SRR4302533.5415435/1‑94 (MQ=60)
                                                         GGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTG                                      >  SRR4302533.1425813/1‑94 (MQ=60)
                                                         GGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGCCGAAGTACCGTCGGAGTTCCGGCAATCGATTG                                      >  SRR4302533.2425386/1‑94 (MQ=60)
                                                          GGCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGG                                     <  SRR4302533.5689329/94‑1 (MQ=60)
                                                           GCTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGG                                    <  SRR4302533.3994623/94‑1 (MQ=60)
                                                            CTCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGG                                   <  SRR4302533.2220930/94‑1 (MQ=60)
                                                             TCTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTGACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGT                                  >  SRR4302533.986346/1‑94 (MQ=60)
                                                              CTTATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTA                                 <  SRR4302533.2836249/94‑1 (MQ=60)
                                                                TATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATC                               <  SRR4302533.937460/94‑1 (MQ=60)
                                                                 ATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGCAGAACCGTGGGAGTTCAGGTAATCGATTGGGGTATCA                              >  SRR4302533.672406/1‑94 (MQ=60)
                                                                 ATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCA                              <  SRR4302533.1961352/94‑1 (MQ=60)
                                                                 ATCGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCA                              <  SRR4302533.1750306/94‑1 (MQ=60)
                                                                   CGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAC                            >  SRR4302533.4055188/1‑94 (MQ=60)
                                                                   CGCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAC                            >  SRR4302533.3592784/1‑94 (MQ=60)
                                                                    GCCGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACG                           <  SRR4302533.1352574/94‑1 (MQ=60)
                                                                      CGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCC                         >  SRR4302533.1860872/1‑94 (MQ=60)
                                                                      CGAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGGTCAGGTAATCGATTGGGGTATCAACGCC                         >  SRR4302533.2214406/1‑94 (MQ=60)
                                                                       GAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCA                        >  SRR4302533.2010566/1‑94 (MQ=60)
                                                                       GAACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCA                        <  SRR4302533.2845394/94‑1 (MQ=60)
                                                                         ACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATG                      <  SRR4302533.3603132/94‑1 (MQ=60)
                                                                         ACACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATG                      >  SRR4302533.5552761/1‑94 (MQ=60)
                                                                          CACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGC                     >  SRR4302533.4595577/1‑94 (MQ=60)
                                                                           ACTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCA                    >  SRR4302533.2159681/1‑94 (MQ=60)
                                                                            CTTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCAT                   >  SRR4302533.684292/1‑94 (MQ=60)
                                                                             TTCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATC                  >  SRR4302533.1253454/1‑94 (MQ=60)
                                                                              TCACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCG                 <  SRR4302533.5229573/94‑1 (MQ=60)
                                                                               CACGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGC                >  SRR4302533.2847811/1‑94 (MQ=60)
                                                                                CCGGATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCC               <  SRR4302533.6017070/94‑1 (MQ=60)
                                                                                    ATAGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCT           <  SRR4302533.3086684/94‑1 (MQ=60)
                                                                                      AGCTGCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTC         <  SRR4302533.2033843/94‑1 (MQ=60)
                                                                                          GCCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGC     >  SRR4302533.2167381/1‑94 (MQ=60)
                                                                                           CCCGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCA    >  SRR4302533.1856440/1‑94 (MQ=60)
                                                                                             CGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGC  >  SRR4302533.1666115/1‑94 (MQ=60)
                                                                                             CGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGC  >  SRR4302533.552605/1‑94 (MQ=60)
                                                                                             CGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGC  <  SRR4302533.889794/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGC  >  NC_000913/2438604‑2438790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑