Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,280 T→C T109A (ACC→GCC)  b2335 ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2800TC100.0% 759.0 / NA 237T109A (ACC→GCC) b2335putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (118/119);  total (118/119)

Only 100 of 241 total aligned reads displayed.

GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449190‑2449369
                                                                                          |                                                                                         
ggTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGa                                                                                         >  1:249176/1‑93 (MQ=255)
 gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        >  2:2858227/1‑93 (MQ=255)
 gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        <  2:5117959/93‑1 (MQ=255)
 gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        >  2:6155166/1‑93 (MQ=255)
 gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        >  1:2349397/1‑93 (MQ=255)
  ttATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGata                                                                                       <  2:4858468/93‑1 (MQ=255)
   tATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGatat                                                                                      <  2:1916816/93‑1 (MQ=255)
   tATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGatat                                                                                      >  1:5015763/1‑93 (MQ=255)
    aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACACTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     >  1:4743537/1‑93 (MQ=255)
    aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     <  2:1661161/93‑1 (MQ=255)
    aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     >  2:127693/1‑93 (MQ=255)
     ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    <  1:2266616/93‑1 (MQ=255)
     ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    >  2:3677455/1‑93 (MQ=255)
     ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    >  2:5730411/1‑93 (MQ=255)
     ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    >  2:525184/1‑93 (MQ=255)
     ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    <  1:98411/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 >  1:682179/1‑93 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  1:5688290/93‑1 (MQ=255)
          atTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACt                                                                               >  2:1037163/1‑93 (MQ=255)
          atTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACt                                                                               >  1:2045474/1‑93 (MQ=255)
           ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              >  2:3478303/1‑93 (MQ=255)
           ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              <  2:2193818/93‑1 (MQ=255)
             aaaTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAg                                                                            <  1:4609532/93‑1 (MQ=255)
                 ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAcc                                                                        >  2:5166126/1‑93 (MQ=255)
                     tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    >  1:2610586/1‑93 (MQ=255)
                        aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGc                                                                 >  1:2371937/1‑93 (MQ=255)
                         gCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGcc                                                                >  1:3748979/1‑93 (MQ=255)
                         gCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGcc                                                                >  2:3889186/1‑93 (MQ=255)
                            aTTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGtt                                                             <  1:3390754/93‑1 (MQ=255)
                            aTTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGtt                                                             >  1:3694422/1‑93 (MQ=255)
                            aTTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGtt                                                             >  2:5852348/1‑93 (MQ=255)
                            aTTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGtt                                                             <  2:3428971/93‑1 (MQ=255)
                                 cACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCg                                                        >  1:135905/1‑93 (MQ=255)
                                  aCCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGt                                                       <  1:6297373/93‑1 (MQ=255)
                                  aCCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGt                                                       >  1:4830203/1‑93 (MQ=255)
                                   ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                      >  1:3849388/1‑93 (MQ=255)
                                    cGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTg                                                     <  2:5431677/93‑1 (MQ=255)
                                     gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                    <  2:3347533/93‑1 (MQ=255)
                                     gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                    <  2:1749955/93‑1 (MQ=255)
                                     gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                    >  1:1551769/1‑93 (MQ=255)
                                     gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                    <  1:2097569/93‑1 (MQ=255)
                                      aCGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAg                                                   >  1:4337715/1‑93 (MQ=255)
                                      aCGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAg                                                   <  1:838127/93‑1 (MQ=255)
                                         gCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCCGCCCGGCGATATTGGTACTGAGTACCGTTTCGTCGCTAATCGTTGAGGTa                                                >  1:3381175/1‑93 (MQ=255)
                                         gCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTa                                                <  2:5926940/93‑1 (MQ=255)
                                          cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               <  2:1989150/93‑1 (MQ=255)
                                          cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               >  1:1894559/1‑93 (MQ=255)
                                           aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                              <  2:693749/93‑1 (MQ=255)
                                           aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                              <  1:1500973/93‑1 (MQ=255)
                                               aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                          >  1:3326420/1‑93 (MQ=255)
                                                aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                         >  2:4359454/1‑93 (MQ=255)
                                                     ttATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                    <  1:2169714/93‑1 (MQ=255)
                                                      tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   <  2:1442892/93‑1 (MQ=255)
                                                      tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   >  2:614725/1‑93 (MQ=255)
                                                      tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   <  1:5266418/93‑1 (MQ=255)
                                                         cGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCa                                <  1:3998536/93‑1 (MQ=255)
                                                         cGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCa                                <  2:2075135/93‑1 (MQ=255)
                                                          ggCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAt                               <  2:3557534/93‑1 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              >  1:914015/1‑93 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              >  1:1297711/1‑93 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              <  2:4649879/93‑1 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              <  1:6026475/93‑1 (MQ=255)
                                                            cgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATcc                             >  1:2964739/1‑93 (MQ=255)
                                                             gcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCg                            >  2:3800964/1‑93 (MQ=255)
                                                              cTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGACCTTAAGTTGCATCCGc                           >  2:1371996/1‑93 (MQ=255)
                                                               tATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCa                          >  2:790979/1‑93 (MQ=255)
                                                                  tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       >  1:4197750/1‑93 (MQ=255)
                                                                   ttCTATCCGAATGCCCAGCGCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTc                      <  2:5511966/93‑1 (MQ=255)
                                                                   ttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTc                      <  1:4881707/93‑1 (MQ=255)
                                                                   ttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGAATTGCCCTTAAGTTGCATCCGCAAGTc                      >  2:4012797/1‑93 (MQ=255)
                                                                    tCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGATGGATTGCCCTTAAGTTGCATCCGCAAGTct                     >  2:3207306/1‑92 (MQ=255)
                                                                    tCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCg                     >  2:5989370/1‑93 (MQ=255)
                                                                     cTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGt                    <  2:3039539/93‑1 (MQ=255)
                                                                      tATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt                   >  1:5134546/1‑93 (MQ=255)
                                                                      tATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt                   <  1:1220281/93‑1 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  <  2:316021/93‑1 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  >  1:1666085/1‑93 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  >  2:6262514/1‑93 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  >  1:5855166/1‑93 (MQ=255)
                                                                        tCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAg                 <  2:4563713/93‑1 (MQ=255)
                                                                        tCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAg                 >  2:2517899/1‑93 (MQ=255)
                                                                          cGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCa               >  2:2275248/1‑93 (MQ=255)
                                                                          cGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCa               >  1:3260078/1‑93 (MQ=255)
                                                                           gAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCaa              <  1:2439878/93‑1 (MQ=255)
                                                                            aaTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg             >  1:3492982/1‑93 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           <  2:3049604/93‑1 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           <  2:5020169/93‑1 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           >  1:5520603/1‑93 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           <  2:1118442/93‑1 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           >  1:1410836/1‑93 (MQ=255)
                                                                               gCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACt          <  1:541353/93‑1 (MQ=255)
                                                                                 ccAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGt        <  1:5742266/93‑1 (MQ=255)
                                                                                  cAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGtt       >  1:6190353/1‑93 (MQ=255)
                                                                                   aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGTCTGttt      >  2:6033717/1‑93 (MQ=255)
                                                                                   aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      >  1:1032009/1‑93 (MQ=255)
                                                                                   aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      >  1:2827815/1‑93 (MQ=255)
                                                                                   aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      <  2:1610533/93‑1 (MQ=255)
                                                                                     cccGGCGATATTGCTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTtgt    <  1:5232191/93‑1 (MQ=255)
                                                                                       cGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGc  >  1:4962161/1‑93 (MQ=255)
                                                                                       cGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGGTAGCAAGACTGTTTGTGc  >  2:4224750/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449190‑2449369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  >  NC_000913/2449188‑2449373
                                                                                            |                                                                                             
CTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCG                                                                                              >  SRR4302533.3037309/1‑94 (MQ=60)
CTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCG                                                                                              <  SRR4302533.5975725/94‑1 (MQ=60)
 TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGA                                                                                             >  SRR4302533.250715/1‑94 (MQ=60)
 TGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGA                                                                                             >  SRR4302533.1452520/1‑94 (MQ=60)
  GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGAT                                                                                            >  SRR4302533.2363932/1‑94 (MQ=60)
   GTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATA                                                                                           >  SRR4302533.3643626/1‑94 (MQ=60)
    TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAT                                                                                          <  SRR4302533.1353472/94‑1 (MQ=60)
    TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAT                                                                                          >  SRR4302533.5046151/1‑94 (MQ=60)
    TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAT                                                                                          >  SRR4302533.2390694/1‑94 (MQ=60)
     TATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACACTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATT                                                                                         >  SRR4302533.4772293/1‑94 (MQ=60)
      ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTG                                                                                        <  SRR4302533.5365603/94‑1 (MQ=60)
      ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTG                                                                                        <  SRR4302533.2274393/94‑1 (MQ=60)
       TTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGG                                                                                       <  SRR4302533.99021/94‑1 (MQ=60)
       TTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGG                                                                                       <  SRR4302533.2280637/94‑1 (MQ=60)
         GATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTA                                                                                     >  SRR4302533.686213/1‑94 (MQ=60)
          ATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAC                                                                                    <  SRR4302533.5722648/94‑1 (MQ=60)
           TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACT                                                                                   <  SRR4302533.4050679/94‑1 (MQ=60)
           TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACT                                                                                   >  SRR4302533.2058084/1‑94 (MQ=60)
             TTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGA                                                                                 <  SRR4302533.3317930/94‑1 (MQ=60)
               AAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGT                                                                               <  SRR4302533.4637447/94‑1 (MQ=60)
                AATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTA                                                                              >  SRR4302533.3381714/1‑94 (MQ=60)
                  TGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACC                                                                            <  SRR4302533.204788/94‑1 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        >  SRR4302533.2542574/1‑94 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        >  SRR4302533.5295975/1‑94 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        >  SRR4302533.2626610/1‑94 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        >  SRR4302533.5796086/1‑94 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        <  SRR4302533.5838622/94‑1 (MQ=60)
                      GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        >  SRR4302533.2369982/1‑94 (MQ=60)
                       TCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTC                                                                       >  SRR4302533.2345255/1‑94 (MQ=60)
                        CCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCG                                                                      >  SRR4302533.155845/1‑94 (MQ=60)
                         CAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGC                                                                     >  SRR4302533.2386614/1‑94 (MQ=60)
                         CAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATATTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGC                                                                     >  SRR4302533.3399013/1‑94 (MQ=60)
                          AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCC                                                                    >  SRR4302533.4871348/1‑94 (MQ=60)
                          AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCC                                                                    <  SRR4302533.4826318/94‑1 (MQ=60)
                          AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCC                                                                    >  SRR4302533.3771963/1‑94 (MQ=60)
                           GCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCG                                                                   <  SRR4302533.5898890/94‑1 (MQ=60)
                           GCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCG                                                                   >  SRR4302533.630713/1‑94 (MQ=60)
                            CTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGT                                                                  >  SRR4302533.3483692/1‑94 (MQ=60)
                             TATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTT                                                                 >  SRR4302533.3717115/1‑94 (MQ=60)
                              ATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTA                                                                >  SRR4302533.4931598/1‑94 (MQ=60)
                              ATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTA                                                                <  SRR4302533.3411694/94‑1 (MQ=60)
                                TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                              >  SRR4302533.1192706/1‑94 (MQ=60)
                                TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                              <  SRR4302533.719463/94‑1 (MQ=60)
                                  TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                            <  SRR4302533.5473116/94‑1 (MQ=60)
                                  TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                            >  SRR4302533.136764/1‑94 (MQ=60)
                                  TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                            >  SRR4302533.655437/1‑94 (MQ=60)
                                  TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                            <  SRR4302533.1474044/94‑1 (MQ=60)
                                   CACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGT                                                           >  SRR4302533.4859479/1‑94 (MQ=60)
                                    ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          >  SRR4302533.2691303/1‑94 (MQ=60)
                                    ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          <  SRR4302533.6334953/94‑1 (MQ=60)
                                    ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          <  SRR4302533.1672069/94‑1 (MQ=60)
                                    ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          >  SRR4302533.3872894/1‑94 (MQ=60)
                                     CCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTG                                                         <  SRR4302533.4968139/94‑1 (MQ=60)
                                      CGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGA                                                        >  SRR4302533.1561112/1‑94 (MQ=60)
                                       GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       >  SRR4302533.4363860/1‑94 (MQ=60)
                                       GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       <  SRR4302533.2110482/94‑1 (MQ=60)
                                       GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       <  SRR4302533.5401516/94‑1 (MQ=60)
                                       GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       <  SRR4302533.182370/94‑1 (MQ=60)
                                        ACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGG                                                      <  SRR4302533.843036/94‑1 (MQ=60)
                                        ACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGG                                                      <  SRR4302533.5561769/94‑1 (MQ=60)
                                          GGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCCGCCCGGCGATATTGGTACTGAGTACCGTTTCGTCGCTAATCGTTGAGGTA                                                    >  SRR4302533.3402045/1‑94 (MQ=60)
                                          GGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTA                                                    >  SRR4302533.3818802/1‑94 (MQ=60)
                                           GCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAT                                                   >  SRR4302533.1906125/1‑94 (MQ=60)
                                           GCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAT                                                   <  SRR4302533.4678976/94‑1 (MQ=60)
                                            CAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATT                                                  >  SRR4302533.31003/1‑94 (MQ=60)
                                             AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                                 <  SRR4302533.1510020/94‑1 (MQ=60)
                                             AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                                 <  SRR4302533.4492192/94‑1 (MQ=60)
                                             AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGCACTGACTACCGTTTCGCCGTTAATCGTTGAGGGATTG                                                 >  SRR4302533.517233/1‑94 (MQ=60)
                                             AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCACGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                                 >  SRR4302533.3405519/1‑94 (MQ=60)
                                              AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCACAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGC                                                <  SRR4302533.6245748/94‑1 (MQ=60)
                                                CAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCC                                              >  SRR4302533.3346975/1‑94 (MQ=60)
                                                CAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGCATTGCCC                                              >  SRR4302533.2404881/1‑94 (MQ=60)
                                                  ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                            <  SRR4302533.4275719/94‑1 (MQ=60)
                                                     AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         >  SRR4302533.958776/1‑94 (MQ=60)
                                                     AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302533.5646582/94‑1 (MQ=60)
                                                     AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302533.3083158/94‑1 (MQ=60)
                                                       TTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTT                                       >  SRR4302533.2465412/1‑94 (MQ=60)
                                                       TTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTT                                       <  SRR4302533.2183058/94‑1 (MQ=60)
                                                       TTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTT                                       >  SRR4302533.2132967/1‑94 (MQ=60)
                                                        TATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTG                                      <  SRR4302533.5298018/94‑1 (MQ=60)
                                                        TATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTG                                      <  SRR4302533.1263677/94‑1 (MQ=60)
                                                         ATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGC                                     >  SRR4302533.904835/1‑94 (MQ=60)
                                                         ATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGC                                     >  SRR4302533.3703935/1‑94 (MQ=60)
                                                           CGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAT                                   <  SRR4302533.4022879/94‑1 (MQ=60)
                                                            GGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATC                                  >  SRR4302533.919390/1‑94 (MQ=60)
                                                            GGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATC                                  >  SRR4302533.1305523/1‑94 (MQ=60)
                                                             GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 <  SRR4302533.5725011/94‑1 (MQ=60)
                                                             GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 <  SRR4302533.6062994/94‑1 (MQ=60)
                                                             GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 >  SRR4302533.2982860/1‑94 (MQ=60)
                                                             GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 >  SRR4302533.2904281/1‑94 (MQ=60)
                                                              CGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCG                                >  SRR4302533.5010654/1‑94 (MQ=60)
                                                               GCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGC                               <  SRR4302533.4644312/94‑1 (MQ=60)
                                                                  ATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAG                            <  SRR4302533.2332882/94‑1 (MQ=60)
                                                                  ATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAG                            >  SRR4302533.1384811/1‑94 (MQ=60)
                                                                   TTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGT                           >  SRR4302533.4223160/1‑94 (MQ=60)
                                                                   TTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGT                           <  SRR4302533.838432/94‑1 (MQ=60)
                                                                     TTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCG                         <  SRR4302533.4911282/94‑1 (MQ=60)
                                                                       CTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                       >  SRR4302533.5165568/1‑94 (MQ=60)
                                                                       CTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                       >  SRR4302533.3847123/1‑94 (MQ=60)
                                                                       CTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                       <  SRR4302533.2076269/94‑1 (MQ=60)
                                                                        TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      >  SRR4302533.5890563/1‑94 (MQ=60)
                                                                        TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      <  SRR4302533.1227606/94‑1 (MQ=60)
                                                                        TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      >  SRR4302533.1676153/1‑94 (MQ=60)
                                                                         ATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG                     <  SRR4302533.2657103/94‑1 (MQ=60)
                                                                          TCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGC                    >  SRR4302533.994965/1‑94 (MQ=60)
                                                                           CCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCA                   >  SRR4302533.3280188/1‑94 (MQ=60)
                                                                             GAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG                 <  SRR4302533.2454946/94‑1 (MQ=60)
                                                                             GAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG                 >  SRR4302533.3514523/1‑94 (MQ=60)
                                                                              AATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGA                <  SRR4302533.1366346/94‑1 (MQ=60)
                                                                               ATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAC               >  SRR4302533.1419366/1‑94 (MQ=60)
                                                                               ATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAC               >  SRR4302533.5553934/1‑94 (MQ=60)
                                                                                 GCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTG             <  SRR4302533.544573/94‑1 (MQ=60)
                                                                                   CCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT           >  SRR4302533.3724958/1‑94 (MQ=60)
                                                                                   CCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT           >  SRR4302533.6227500/1‑94 (MQ=60)
                                                                                   CCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT           <  SRR4302533.5776916/94‑1 (MQ=60)
                                                                                    CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          <  SRR4302533.393138/94‑1 (MQ=60)
                                                                                    CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          >  SRR4302533.2845215/1‑94 (MQ=60)
                                                                                    CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          >  SRR4302533.2071045/1‑94 (MQ=60)
                                                                                    CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          >  SRR4302533.1038077/1‑94 (MQ=60)
                                                                                     AGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG         <  SRR4302533.4277154/94‑1 (MQ=60)
                                                                                     AGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG         >  SRR4302533.5094975/1‑94 (MQ=60)
                                                                                     AGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTG         <  SRR4302533.1770671/94‑1 (MQ=60)
                                                                                       CCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTG       <  SRR4302533.5117432/94‑1 (MQ=60)
                                                                                       CCCGGCGATATTGCTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTG       <  SRR4302533.5263658/94‑1 (MQ=60)
                                                                                        CCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC      <  SRR4302533.6198498/94‑1 (MQ=60)
                                                                                        CCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC      >  SRR4302533.4992247/1‑94 (MQ=60)
                                                                                         CGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCG     >  SRR4302533.1859864/1‑94 (MQ=60)
                                                                                          GGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAG    <  SRR4302533.3523472/94‑1 (MQ=60)
                                                                                            CGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  <  SRR4302533.6223898/94‑1 (MQ=60)
                                                                                            CGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  <  SRR4302533.239565/94‑1 (MQ=60)
                                                                                            |                                                                                             
CTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  >  NC_000913/2449188‑2449373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑