Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,458,650 +G intergenic (‑161/+20) b2342 ← / ← b2343 putative acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,458,6461.G100.0% 558.4 / NA 173intergenic (‑157/+24)b2342/b2343putative acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (106/67);  total (106/67)

Only 100 of 193 total aligned reads displayed.

ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATC  >  NC_000913/2458554‑2458732
                                                                                             |                                                                                       
aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACa                                                                                          >  1:1594137/1‑93 (MQ=255)
  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                      >  1:5350685/1‑93 (MQ=255)
  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑g                                                                                      <  2:657951/93‑1 (MQ=255)
   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑gg                                                                                     >  2:3669297/1‑93 (MQ=255)
   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑gg                                                                                     >  2:3888712/1‑93 (MQ=255)
   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑gg                                                                                     >  1:2144423/1‑93 (MQ=255)
        ccAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑g‑ggggcgt                                                                                >  1:241041/1‑89 (MQ=255)
          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGcgtac                                                                               <  1:5760100/93‑1 (MQ=255)
           aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGcgtacg                                                                              >  2:2608670/1‑93 (MQ=255)
              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCgtacgtac                                                                           >  1:177652/1‑93 (MQ=255)
              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCgtacgtac                                                                           <  2:5249494/93‑1 (MQ=255)
              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCgtacgtac                                                                           <  2:4125882/93‑1 (MQ=255)
              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCgtacgtac                                                                           >  2:45713/1‑93 (MQ=255)
               gagaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTAcc                                                                          >  2:4053811/1‑93 (MQ=255)
                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTAccc                                                                         <  2:6213932/93‑1 (MQ=255)
                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTAccc                                                                         <  1:3922252/93‑1 (MQ=255)
                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTAccc                                                                         >  2:4860713/1‑93 (MQ=255)
                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCgaga                                                                     >  2:1014128/1‑93 (MQ=255)
                     aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGagag                                                                    <  1:1460028/93‑1 (MQ=255)
                       ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGa                                                                  <  1:1739170/93‑1 (MQ=255)
                       ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGa                                                                  >  1:1230236/1‑93 (MQ=255)
                        gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGaa                                                                 >  2:1654151/1‑93 (MQ=255)
                        gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGaa                                                                 >  1:2652425/1‑93 (MQ=255)
                        gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGaa                                                                 <  1:5805867/93‑1 (MQ=255)
                         aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAAt                                                                >  2:68243/1‑93 (MQ=255)
                            gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTaa                                                             <  2:3761573/93‑1 (MQ=255)
                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACg                                                           >  1:1152473/1‑93 (MQ=255)
                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACg                                                           >  1:3772215/1‑93 (MQ=255)
                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACg                                                           >  1:1419610/1‑93 (MQ=255)
                               ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGc                                                          >  2:4839028/1‑93 (MQ=255)
                               ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGc                                                          >  2:4556762/1‑93 (MQ=255)
                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCa                                                         >  1:4406811/1‑93 (MQ=255)
                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCa                                                         <  1:588439/93‑1 (MQ=255)
                                  gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                       >  2:3354561/1‑93 (MQ=255)
                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                     <  2:5169976/93‑1 (MQ=255)
                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                     >  1:281385/1‑93 (MQ=255)
                                     aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCATAccc                                                    >  2:5385767/1‑93 (MQ=255)
                                     aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                    >  2:2118456/1‑93 (MQ=255)
                                      tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                   >  1:6258328/1‑93 (MQ=255)
                                      tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                   <  1:3683906/93‑1 (MQ=255)
                                      tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                   <  2:4860232/93‑1 (MQ=255)
                                          acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                               >  1:4363091/1‑93 (MQ=255)
                                           caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTgg                                              >  2:2466430/1‑93 (MQ=255)
                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                            >  1:4156456/1‑93 (MQ=255)
                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                            >  1:2972749/1‑93 (MQ=255)
                                              agaaaAAAAAAAAAGAGAAAAAAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                           <  2:1225831/93‑1 (MQ=255)
                                               aaaaaaaGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                          <  1:1122548/90‑1 (MQ=255)
                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                        >  2:956754/1‑93 (MQ=255)
                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                        >  1:4222264/1‑93 (MQ=255)
                                                  aaaagaaaaagaAAAAAAAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                       <  1:1188400/93‑1 (MQ=255)
                                                    aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGc                                     >  1:4099846/1‑93 (MQ=255)
                                                    aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGc                                     >  2:855433/1‑93 (MQ=255)
                                                     agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                    <  2:1604947/93‑1 (MQ=255)
                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                   >  1:1922218/1‑93 (MQ=255)
                                                      aacaaagaGAAAAAAACACGCCCCGGCGAGCAACTGACA‑GCGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCAt                                   <  1:2519600/89‑1 (MQ=255)
                                                      aaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                   <  2:1359396/92‑1 (MQ=255)
                                                       aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  <  1:4274008/93‑1 (MQ=255)
                                                       aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  1:5232275/1‑93 (MQ=255)
                                                       aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  2:4994468/1‑93 (MQ=255)
                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAttt                                 <  1:601957/93‑1 (MQ=255)
                                                         gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                <  2:1756693/93‑1 (MQ=255)
                                                         gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCCGGAAAATCAGCATTTc                                >  2:3528114/1‑93 (MQ=255)
                                                            gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                             >  2:5885202/1‑93 (MQ=255)
                                                            gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                             >  2:278484/1‑93 (MQ=255)
                                                              gaaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCtt                           <  1:6262448/93‑1 (MQ=255)
                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                          <  2:2163054/93‑1 (MQ=255)
                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                          >  1:3003444/1‑93 (MQ=255)
                                                               aaaagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                          <  2:2574747/93‑1 (MQ=255)
                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                        <  1:3817007/93‑1 (MQ=255)
                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                        >  1:4704907/1‑93 (MQ=255)
                                                                   gaaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                      <  2:426744/93‑1 (MQ=255)
                                                                    aaaaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                     >  2:5743730/1‑93 (MQ=255)
                                                                      aaCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCa                   >  1:4851517/1‑93 (MQ=255)
                                                                         gCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                >  2:3103509/1‑93 (MQ=255)
                                                                         gCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                >  2:5429437/1‑93 (MQ=255)
                                                                          ccccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg               <  2:1928669/93‑1 (MQ=255)
                                                                          ccccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg               <  1:5938760/93‑1 (MQ=255)
                                                                          ccccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg               >  1:6298162/1‑93 (MQ=255)
                                                                           cccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              <  1:6143025/93‑1 (MQ=255)
                                                                           cccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              <  1:4860713/93‑1 (MQ=255)
                                                                           cccTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              >  1:5951926/1‑93 (MQ=255)
                                                                             cTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            <  2:6034501/93‑1 (MQ=255)
                                                                             cTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            >  1:2732386/1‑93 (MQ=255)
                                                                             cTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            <  2:1407099/93‑1 (MQ=255)
                                                                              tGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa           >  1:5080276/1‑93 (MQ=255)
                                                                              tGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa           <  1:743349/93‑1 (MQ=255)
                                                                              tGCCAGCAACTGACAGG‑GGGCGGACGTACCCGATAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa           >  1:2890715/1‑93 (MQ=255)
                                                                                ccAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt         <  1:646895/93‑1 (MQ=255)
                                                                                ccAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt         >  2:5925913/1‑93 (MQ=255)
                                                                                 cggCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc        <  1:2396327/91‑1 (MQ=255)
                                                                                 cAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTGGCAGGCAAAAGTGAAATc        >  1:2494226/1‑93 (MQ=255)
                                                                                 cAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc        >  2:2927302/1‑93 (MQ=255)
                                                                                  aGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg       <  2:4700594/93‑1 (MQ=255)
                                                                                   gCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa      <  1:3509709/93‑1 (MQ=255)
                                                                                    cAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat     >  1:1029276/1‑93 (MQ=255)
                                                                                     aaCTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata    >  2:4752832/1‑93 (MQ=255)
                                                                                      aCTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAATTGAAATCGatat   <  1:6063116/93‑1 (MQ=255)
                                                                                      aCTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat   >  2:3648264/1‑93 (MQ=255)
                                                                                      aCTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTCCAGCTTCGCAGGCAAAAGTGAAATCGatat   <  1:3150330/93‑1 (MQ=255)
                                                                                       cTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc  <  1:4474055/93‑1 (MQ=255)
                                                                                             |                                                                                       
ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATC  >  NC_000913/2458554‑2458732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCAC  >  NC_000913/2458557‑2458741
                                                                                               |                                                                                           
TTTAGCCAGGTGGAGAAAAGGAAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAAAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGG                                                                                             <  SRR4302533.1316553/94‑1 (MQ=60)
   AGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGgcg                                                                                          >  SRR4302533.6071072/1‑91 (MQ=60)
   AGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGgcg                                                                                          <  SRR4302533.3156959/94‑4 (MQ=60)
    GCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGT                                                                                          >  SRR4302533.242538/1‑94 (MQ=60)
      CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTAC                                                                                        >  SRR4302533.6339563/1‑94 (MQ=60)
      CAAGTGAAAAAAAGGAAAGGGGGGAGCTATGACACAGAGAAAAAAGAAGAAGAGAAAAAAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTAC                                                                                        <  SRR4302533.1225837/94‑1 (MQ=60)
       AAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACG                                                                                       <  SRR4302533.5794857/94‑1 (MQ=60)
         GTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTA                                                                                     >  SRR4302533.4746202/1‑94 (MQ=60)
         GTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTA                                                                                     >  SRR4302533.1192637/1‑94 (MQ=60)
          TGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTAC                                                                                    >  SRR4302533.178765/1‑94 (MQ=60)
           GGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACC                                                                                   <  SRR4302533.2867440/94‑1 (MQ=60)
             AGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCG                                                                                 <  SRR4302533.3946204/94‑1 (MQ=60)
               AAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAG                                                                               >  SRR4302533.2428976/1‑94 (MQ=60)
               AAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAG                                                                               <  SRR4302533.1779249/94‑1 (MQ=60)
                AAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGA                                                                              >  SRR4302533.3876553/1‑94 (MQ=60)
                  AGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGG                                                                            <  SRR4302533.1468829/94‑1 (MQ=60)
                   GGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGA                                                                           >  SRR4302533.1237599/1‑94 (MQ=60)
                    GGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAA                                                                          >  SRR4302533.2668720/1‑94 (MQ=60)
                    GGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAA                                                                          <  SRR4302533.1749718/94‑1 (MQ=60)
                     GAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAAT                                                                         <  SRR4302533.5840925/94‑1 (MQ=60)
                         GTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAAC                                                                     >  SRR4302533.3814048/1‑94 (MQ=60)
                         GTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAAC                                                                     <  SRR4302533.2954000/94‑1 (MQ=60)
                         GGGGGAGCTAAGACAGAGAGAGAAAAGAGGAAGATAAAAGAAACCGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAAC                                                                     <  SRR4302533.3764645/94‑1 (MQ=60)
                          TGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACG                                                                    >  SRR4302533.3795337/1‑94 (MQ=60)
                          TGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACG                                                                    >  SRR4302533.1428200/1‑94 (MQ=60)
                          TGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACG                                                                    >  SRR4302533.1159375/1‑94 (MQ=60)
                            GGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCA                                                                  >  SRR4302533.4433422/1‑94 (MQ=60)
                             GAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAG                                                                 <  SRR4302533.591913/94‑1 (MQ=60)
                              AGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGA                                                                >  SRR4302533.5406456/1‑94 (MQ=60)
                              AGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGA                                                                <  SRR4302533.745435/94‑1 (MQ=60)
                               GCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGAC                                                               >  SRR4302533.877931/1‑94 (MQ=60)
                                CTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACC                                                              >  SRR4302533.283106/1‑94 (MQ=60)
                                 TATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCC                                                             >  SRR4302533.4284517/1‑94 (MQ=60)
                                  ATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCA                                                            >  SRR4302533.6295752/1‑94 (MQ=60)
                                   TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAG                                                           <  SRR4302533.3706548/94‑1 (MQ=60)
                                     ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCT                                                         <  SRR4302533.6296467/94‑1 (MQ=60)
                                      CACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTG                                                        >  SRR4302533.4389429/1‑94 (MQ=60)
                                       ACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGG                                                       <  SRR4302533.603240/94‑1 (MQ=60)
                                        CAGAGAGAAAATAAGAAGAGAAAGGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGA                                                      <  SRR4302533.5819359/94‑1 (MQ=60)
                                         AGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAA                                                     >  SRR4302533.4181637/1‑94 (MQ=60)
                                         AGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAA                                                     >  SRR4302533.2990940/1‑94 (MQ=60)
                                           AGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAA                                                   >  SRR4302533.6158853/1‑94 (MQ=60)
                                            aaaAAAAGAAGAAAAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAT                                                  <  SRR4302533.1129211/91‑1 (MQ=60)
                                             AGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATC                                                 >  SRR4302533.4247793/1‑94 (MQ=60)
                                               AAAAGAAAAAGAAAAAAAAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAG                                               <  SRR4302533.1195534/94‑1 (MQ=60)
                                                AAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGC                                              >  SRR4302533.4124797/1‑94 (MQ=60)
                                                 AAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCA                                             <  SRR4302533.5277732/94‑1 (MQ=60)
                                                  AGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAT                                            >  SRR4302533.1933950/1‑94 (MQ=60)
                                                   aacaAAGAGAAAAAAACACGCCCCGGCGAGCAACTGACA‑GCGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATT                                           <  SRR4302533.2535125/90‑1 (MQ=60)
                                                   aaaaAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATT                                           <  SRR4302533.420240/90‑1 (MQ=60)
                                                   GAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATT                                           >  SRR4302533.5263743/1‑94 (MQ=60)
                                                    AAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTT                                          <  SRR4302533.4299724/94‑1 (MQ=60)
                                                    AAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTT                                          >  SRR4302533.5939258/1‑94 (MQ=60)
                                                     AGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTC                                         >  SRR4302533.1207303/1‑94 (MQ=60)
                                                     AGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTC                                         <  SRR4302533.605496/94‑1 (MQ=60)
                                                     AGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTC                                         <  SRR4302533.3623087/94‑1 (MQ=60)
                                                         GGGAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCACCATTTCAGCT                                     >  SRR4302533.5981647/1‑94 (MQ=60)
                                                           GAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTC                                   >  SRR4302533.3021814/1‑94 (MQ=60)
                                                           GAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTC                                   <  SRR4302533.6299884/94‑1 (MQ=60)
                                                           GAAAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTC                                   <  SRR4302533.449428/94‑1 (MQ=60)
                                                             AAAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGC                                 >  SRR4302533.4733424/1‑94 (MQ=60)
                                                              AAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                                <  SRR4302533.3840344/94‑1 (MQ=60)
                                                              AAGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                                >  SRR4302533.2998136/1‑94 (MQ=60)
                                                               AGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                               <  SRR4302533.3417970/94‑1 (MQ=60)
                                                               AGAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                               <  SRR4302533.2649626/94‑1 (MQ=60)
                                                                GAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                              >  SRR4302533.6319220/1‑94 (MQ=60)
                                                                GAAAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                              <  SRR4302533.624731/94‑1 (MQ=60)
                                                                  AAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCA                            >  SRR4302533.1809260/1‑94 (MQ=60)
                                                                  AAACGCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCA                            >  SRR4302533.4880917/1‑94 (MQ=60)
                                                                      GCCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAG                        >  SRR4302533.6335755/1‑94 (MQ=60)
                                                                       CCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                       >  SRR4302533.5987930/1‑94 (MQ=60)
                                                                       CCCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGT                       <  SRR4302533.5974667/94‑1 (MQ=60)
                                                                        CCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                      <  SRR4302533.4890173/94‑1 (MQ=60)
                                                                        CCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                      <  SRR4302533.4890174/94‑1 (MQ=60)
                                                                        CCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                      >  SRR4302533.4894205/1‑94 (MQ=60)
                                                                        CCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                      <  SRR4302533.6180016/94‑1 (MQ=60)
                                                                        CCCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCAAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                      <  SRR4302533.2715037/94‑1 (MQ=60)
                                                                         CCTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGA                     >  SRR4302533.2749151/1‑94 (MQ=60)
                                                                          CTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA                    >  SRR4302533.5110982/1‑94 (MQ=60)
                                                                          CTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA                    >  SRR4302533.4348197/1‑94 (MQ=60)
                                                                          CTGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA                    >  SRR4302533.3975308/1‑94 (MQ=60)
                                                                          CTGCCAGCAACTGACAGG‑GGGCGGACGTACCCGATAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA                    >  SRR4302533.2908484/1‑94 (MQ=60)
                                                                           TGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA                   <  SRR4302533.747725/94‑1 (MQ=60)
                                                                           TGCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA                   >  SRR4302533.1138117/1‑94 (MQ=60)
                                                                            GCCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAT                  >  SRR4302533.1560623/1‑94 (MQ=60)
                                                                             CCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTGGCAGGCAAAAGTGAAATC                 >  SRR4302533.2509613/1‑94 (MQ=60)
                                                                             CCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC                 <  SRR4302533.650707/94‑1 (MQ=60)
                                                                             CCAGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC                 >  SRR4302533.44118/1‑94 (MQ=60)
                                                                              CGGCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG                <  SRR4302533.2411119/94‑1 (MQ=60)
                                                                                GCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGAT              >  SRR4302533.1035324/1‑94 (MQ=60)
                                                                                GCAACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGAT              <  SRR4302533.3531345/94‑1 (MQ=60)
                                                                                  AACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATAT            <  SRR4302533.2041719/94‑1 (MQ=60)
                                                                                  AACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATAT            >  SRR4302533.1611714/1‑94 (MQ=60)
                                                                                  AACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATAT            <  SRR4302533.1459208/94‑1 (MQ=60)
                                                                                   ACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAATTGAAATCGATATC           <  SRR4302533.6099820/94‑1 (MQ=60)
                                                                                   ACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATC           >  SRR4302533.2206273/1‑94 (MQ=60)
                                                                                   ACTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTCCAGCTTCGCAGGCAAAAGTGAAATCGATATC           <  SRR4302533.3169701/94‑1 (MQ=60)
                                                                                    CTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCC          <  SRR4302533.2412774/94‑1 (MQ=60)
                                                                                    CTGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCC          <  SRR4302533.4501113/94‑1 (MQ=60)
                                                                                     TGACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA         >  SRR4302533.5773491/1‑94 (MQ=60)
                                                                                       ACAGG‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGG       <  SRR4302533.66342/94‑1 (MQ=60)
                                                                                         G‑G‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGC    >  SRR4302533.5656446/1‑94 (MQ=60)
                                                                                             GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTAAGCTTCGCAGGCGAAAGTGAAATCGATATCCAGGCGCAC  >  SRR4302533.1997841/1‑94 (MQ=60)
                                                                                               |                                                                                           
TTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACA‑G‑GGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCAC  >  NC_000913/2458557‑2458741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑