Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,560,887	T→C	V115A (GTG→GCG)	b2447 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,560,887	0	T	C	99.6%	795.1 / NA	250	V115A (GTG→GCG)	b2447	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (120/129); minor base A (1/0); total (121/129)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGG  >  NC_000913/2560797‑2560975

 tCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCt                                                                                       <  2:3102759/93‑1 (MQ=255)

 tCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCt                                                                                       >  2:4832288/1‑93 (MQ=255)

 tCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCt                                                                                       <  2:3488029/93‑1 (MQ=255)

 tCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCt                                                                                       >  1:547850/1‑93 (MQ=255)

   cAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTg                                                                                     >  2:2165585/1‑93 (MQ=255)

   cAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTg                                                                                     <  2:2257973/93‑1 (MQ=255)

   cAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAAACCGATTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTg                                                                                     >  1:2306578/1‑93 (MQ=255)

    aGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGc                                                                                    >  2:5561738/1‑93 (MQ=255)

    aGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGc                                                                                    >  2:5009448/1‑93 (MQ=255)

    aGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGc                                                                                    >  1:3492373/1‑93 (MQ=255)

      aaTATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCt                                                                                  >  1:2407157/1‑93 (MQ=255)

      aaTATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCt                                                                                  <  1:1965651/93‑1 (MQ=255)

      aaTATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCt                                                                                  <  2:5515845/93‑1 (MQ=255)

      aaTATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCt                                                                                  >  2:3393374/1‑93 (MQ=255)

       atatGCGAAGCACTGGAACATGAAAGGCGTCGTGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTg                                                                                 <  2:4965503/93‑1 (MQ=255)

       atatGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTGCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTg                                                                                 <  1:4435124/93‑1 (MQ=255)

        tatGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTgg                                                                                <  2:5363529/93‑1 (MQ=255)

         atacGAAGCACTGAAACATGAAAGGCGTCATGAATGACAAAGCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTggg                                                                               <  1:2371735/90‑1 (MQ=255)

         atGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTggg                                                                               >  2:4315722/1‑93 (MQ=255)

           gCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTg                                                                             <  1:2524829/93‑1 (MQ=255)

            cGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAACCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGa                                                                            <  1:2118534/93‑1 (MQ=255)

             gAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAg                                                                           >  2:6017358/1‑93 (MQ=255)

             gAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAg                                                                           >  1:5749495/1‑93 (MQ=255)

               aGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCt                                                                         <  1:4757582/93‑1 (MQ=255)

               aGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCt                                                                         >  2:6185126/1‑93 (MQ=255)

                gCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCtt                                                                        <  1:5116978/93‑1 (MQ=255)

                gCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCtt                                                                        >  2:2814103/1‑93 (MQ=255)

                gCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCtt                                                                        <  2:1513986/93‑1 (MQ=255)

                 gaCTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTa                                                                       <  1:451048/92‑1 (MQ=255)

                 cACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTa                                                                       <  2:5593915/93‑1 (MQ=255)

                      gAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGca                                                                  >  2:5319847/1‑93 (MQ=255)

                       aaCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcac                                                                 >  1:4200099/1‑93 (MQ=255)

                       aaCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcac                                                                 <  1:1838630/93‑1 (MQ=255)

                        aCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGcaca                                                                >  1:6185447/1‑93 (MQ=255)

                         cATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACaa                                                               >  1:5032639/1‑93 (MQ=255)

                          aTGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACaaa                                                              >  1:1582046/1‑93 (MQ=255)

                          aTGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACaaa                                                              >  1:805769/1‑93 (MQ=255)

                          aTGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACaaa                                                              <  1:712871/93‑1 (MQ=255)

                            gAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGa                                                            >  2:3463189/1‑93 (MQ=255)

                             aaaGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGac                                                           <  1:1439287/93‑1 (MQ=255)

                               aGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAGGacac                                                         <  1:1406302/93‑1 (MQ=255)

                                 gCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCt                                                       <  2:1174259/93‑1 (MQ=255)

                                   gTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTc                                                     <  1:1554537/93‑1 (MQ=255)

                                    tCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCa                                                    >  1:440601/1‑93 (MQ=255)

                                     cATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAc                                                   <  1:4107114/93‑1 (MQ=255)

                                     cATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAc                                                   <  2:5020541/93‑1 (MQ=255)

                                     cATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAc                                                   <  1:3540241/93‑1 (MQ=255)

                                      atgaatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACg                                                  >  1:3899497/1‑93 (MQ=255)

                                        gaatgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACggg                                                <  1:4585014/93‑1 (MQ=255)

                                          atgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTg                                              >  2:1248381/1‑93 (MQ=255)

                                          atgaCAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGCACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTg                                              >  1:2992572/1‑93 (MQ=255)

                                              cAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGaa                                          >  1:2772792/1‑93 (MQ=255)

                                               aaaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGaaa                                         <  2:1813596/93‑1 (MQ=255)

                                               aaaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGaaa                                         <  2:1280472/93‑1 (MQ=255)

                                                aaccccGCTTCCACGCCGACAAGTGGGTAGGGTACGGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAc                                        <  2:4458209/90‑1 (MQ=255)

                                                aaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAc                                        >  2:2108702/1‑93 (MQ=255)

                                                aaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAc                                        <  1:5043669/93‑1 (MQ=255)

                                                aaaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAc                                        >  1:2459292/1‑93 (MQ=255)

                                                 aaCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACg                                       <  2:3674921/93‑1 (MQ=255)

                                                   cccGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGt                                     <  2:900449/93‑1 (MQ=255)

                                                    ccGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGtt                                    <  1:953023/93‑1 (MQ=255)

                                                      gCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTc                                  >  1:39416/1‑93 (MQ=255)

                                                       cTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCa                                 >  1:2911062/1‑93 (MQ=255)

                                                       cTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCa                                 >  1:5308700/1‑93 (MQ=255)

                                                        ttCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCaa                                >  1:3118788/1‑93 (MQ=255)

                                                        ttCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCaa                                >  1:5375859/1‑93 (MQ=255)

                                                        ttCCACGCCGACAAGTGGGTAGGGTACAGCAAGGAGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCaa                                >  2:4178916/1‑93 (MQ=255)

                                                         tCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAAt                               <  1:2052301/93‑1 (MQ=255)

                                                         tCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAAt                               <  1:1075063/93‑1 (MQ=255)

                                                         tCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAAt                               >  2:3904100/1‑93 (MQ=255)

                                                          cctcGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATa                              <  2:2463514/90‑1 (MQ=255)

                                                          ccACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATa                              <  2:1405284/93‑1 (MQ=255)

                                                           cACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATAc                             >  1:6223322/1‑93 (MQ=255)

                                                                cGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCa                        >  1:1211887/1‑93 (MQ=255)

                                                                cGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCa                        >  1:4020855/1‑93 (MQ=255)

                                                                 gACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCaa                       >  2:112803/1‑93 (MQ=255)

                                                                  aCAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCaaa                      <  2:2581612/93‑1 (MQ=255)

                                                                   cAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAAc                     >  2:383495/1‑93 (MQ=255)

                                                                   cAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAAc                     <  1:3281454/93‑1 (MQ=255)

                                                                     aGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGATTCAATACTTCCAAACGa                   >  2:5886769/1‑93 (MQ=255)

                                                                       tGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCCAACGATg                 >  2:3760446/1‑93 (MQ=255)

                                                                       tGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATg                 <  1:4662512/93‑1 (MQ=255)

                                                                        gggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGt                <  1:6018779/93‑1 (MQ=255)

                                                                        gggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGt                >  1:5435818/1‑93 (MQ=255)

                                                                        gggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGt                >  2:1627004/1‑93 (MQ=255)

                                                                        gggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGt                <  2:4794308/93‑1 (MQ=255)

                                                                         ggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTa               <  2:3535673/93‑1 (MQ=255)

                                                                         ggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTa               <  2:3260812/93‑1 (MQ=255)

                                                                         ggtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTa               <  1:2645346/93‑1 (MQ=255)

                                                                          gtagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTAc              <  2:1708508/93‑1 (MQ=255)

                                                                           tagggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACg             >  2:3076311/1‑93 (MQ=255)

                                                                              ggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACa          >  2:229154/1‑93 (MQ=255)

                                                                              ggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACa          <  1:2562647/93‑1 (MQ=255)

                                                                              ggtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACa          >  2:646689/1‑93 (MQ=255)

                                                                               gtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACAt         >  2:2869952/1‑93 (MQ=255)

                                                                               gtaCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACAt         >  1:2867830/1‑93 (MQ=255)

                                                                                taCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATg        <  2:4616348/93‑1 (MQ=255)

                                                                                taCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATg        >  2:4350555/1‑93 (MQ=255)

                                                                                 aCAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATgg       >  1:1662214/1‑93 (MQ=255)

                                                                                    gCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAAc    >  2:4977064/1‑93 (MQ=255)

ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGG  >  NC_000913/2560797‑2560975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGC  >  NC_000913/2560794‑2560976

TGCATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGC                                                                                           <  SRR4302533.2604915/94‑1 (MQ=60)

 GCATCCAGAATATGCGAAGCACTGGAACATGAAGGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCG                                                                                          <  SRR4302533.1245772/94‑1 (MQ=60)

 GCATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCG                                                                                          <  SRR4302533.2791546/94‑1 (MQ=60)

   ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCT                                                                                        >  SRR4302533.2101472/1‑94 (MQ=60)

   ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCT                                                                                        >  SRR4302533.551102/1‑94 (MQ=60)

   ATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCT                                                                                        <  SRR4302533.5341536/94‑1 (MQ=60)

    TCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTT                                                                                       <  SRR4302533.4040315/94‑1 (MQ=60)

    TCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTT                                                                                       <  SRR4302533.5422282/94‑1 (MQ=60)

     CCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTG                                                                                      <  SRR4302533.4512113/94‑1 (MQ=60)

     CCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTG                                                                                      <  SRR4302533.5417422/94‑1 (MQ=60)

     CCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAAACCGATTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTG                                                                                      >  SRR4302533.2320868/1‑94 (MQ=60)

      CAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGC                                                                                     >  SRR4302533.3513909/1‑94 (MQ=60)

       AGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCC                                                                                    <  SRR4302533.6279819/94‑1 (MQ=60)

        GAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCT                                                                                   <  SRR4302533.4376935/94‑1 (MQ=60)

        GAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCT                                                                                   >  SRR4302533.2422026/1‑94 (MQ=60)

         AATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAACCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTG                                                                                  <  SRR4302533.444639/94‑1 (MQ=60)

         AATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTG                                                                                  <  SRR4302533.1977661/94‑1 (MQ=60)

          ATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTGCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGG                                                                                 <  SRR4302533.4461933/94‑1 (MQ=60)

            ATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGT                                                                               <  SRR4302533.2552491/94‑1 (MQ=60)

            ATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGT                                                                               <  SRR4302533.5742456/94‑1 (MQ=60)

            ATACGAAGCACTGAAACATGAAAGGCGTCATGAATGACAAAGCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGT                                                                               <  SRR4302533.2386410/94‑1 (MQ=60)

              GCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGA                                                                             <  SRR4302533.2540377/94‑1 (MQ=60)

               CGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAACCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAG                                                                            <  SRR4302533.2131600/94‑1 (MQ=60)

               CGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAG                                                                            >  SRR4302533.5784180/1‑94 (MQ=60)

                  AGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTT                                                                         <  SRR4302533.4786448/94‑1 (MQ=60)

                   GCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTA                                                                        <  SRR4302533.5147901/94‑1 (MQ=60)

                    GACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTAT                                                                       <  SRR4302533.453747/94‑1 (MQ=60)

                    CACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTAT                                                                       <  SRR4302533.2957234/94‑1 (MQ=60)

                    CACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTAT                                                                       >  SRR4302533.1440419/1‑94 (MQ=60)

                     ACTGGAACATGAAAGGCGTCATGAATGACAAATCCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATC                                                                      <  SRR4302533.3835954/94‑1 (MQ=60)

                      CTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCG                                                                     <  SRR4302533.6258201/94‑1 (MQ=60)

                      CTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCG                                                                     <  SRR4302533.5479557/94‑1 (MQ=60)

                         GAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCAC                                                                  >  SRR4302533.4225514/1‑94 (MQ=60)

                          AACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACA                                                                 >  SRR4302533.6222580/1‑94 (MQ=60)

                          AACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACA                                                                 <  SRR4302533.1849843/94‑1 (MQ=60)

                           TCATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAA                                                                <  SRR4302533.6084857/94‑1 (MQ=60)

                           ACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAA                                                                >  SRR4302533.5063127/1‑94 (MQ=60)

                            CATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAA                                                               >  SRR4302533.810504/1‑94 (MQ=60)

                            CATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAA                                                               >  SRR4302533.1591578/1‑94 (MQ=60)

                             ATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAG                                                              >  SRR4302533.717106/1‑94 (MQ=60)

                             ATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAG                                                              <  SRR4302533.717078/94‑1 (MQ=60)

                             ATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAA                                                              >  SRR4302533.1858534/1‑94 (MQ=60)

                              TGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGA                                                             >  SRR4302533.2808312/1‑94 (MQ=60)

                              TGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGA                                                             >  SRR4302533.4713232/1‑94 (MQ=60)

                              TGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGA                                                             >  SRR4302533.5166765/1‑94 (MQ=60)

                                AAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACA                                                           >  SRR4302533.2599664/1‑94 (MQ=60)

                                AAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACA                                                           >  SRR4302533.884013/1‑94 (MQ=60)

                                AAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACA                                                           <  SRR4302533.1447973/94‑1 (MQ=60)

                                  AGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAGGACACC                                                         <  SRR4302533.1414807/94‑1 (MQ=60)

                                  AGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACC                                                         <  SRR4302533.1509614/94‑1 (MQ=60)

                                   GGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCT                                                        <  SRR4302533.3129285/94‑1 (MQ=60)

                                   GGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCT                                                        >  SRR4302533.2092176/1‑94 (MQ=60)

                                   GGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCT                                                        <  SRR4302533.1209862/94‑1 (MQ=60)

                                    GCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTT                                                       <  SRR4302533.2343824/94‑1 (MQ=60)

                                    GCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTT                                                       <  SRR4302533.5910470/94‑1 (MQ=60)

                                      GTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCA                                                     <  SRR4302533.1551004/94‑1 (MQ=60)

                                      GTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCA                                                     >  SRR4302533.443232/1‑94 (MQ=60)

                                      GTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCA                                                     <  SRR4302533.2813919/94‑1 (MQ=60)

                                      GTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCA                                                     <  SRR4302533.1563896/94‑1 (MQ=60)

                                      GTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGGGAGCTTATCGCACAAAGACACCTTCA                                                     >  SRR4302533.2407213/1‑94 (MQ=60)

                                       TCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAC                                                    >  SRR4302533.3367285/1‑94 (MQ=60)

                                       TCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAC                                                    <  SRR4302533.567271/94‑1 (MQ=60)

                                       TCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAC                                                    <  SRR4302533.46087/94‑1 (MQ=60)

                                       TCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAC                                                    <  SRR4302533.2515343/94‑1 (MQ=60)

                                       TCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCAC                                                    <  SRR4302533.2018182/94‑1 (MQ=60)

                                        CATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACG                                                   <  SRR4302533.4132087/94‑1 (MQ=60)

                                        CATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACG                                                   <  SRR4302533.4132086/94‑1 (MQ=60)

                                        CATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACG                                                   >  SRR4302533.3923292/1‑94 (MQ=60)

                                        CATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACG                                                   <  SRR4302533.3562055/94‑1 (MQ=60)

                                        CATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACG                                                   <  SRR4302533.3468842/94‑1 (MQ=60)

                                          TGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGG                                                 >  SRR4302533.3941649/1‑94 (MQ=60)

                                           GAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGT                                                <  SRR4302533.4612787/94‑1 (MQ=60)

                                           GAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGT                                                >  SRR4302533.2411413/1‑94 (MQ=60)

                                           GAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGT                                                <  SRR4302533.4664064/94‑1 (MQ=60)

                                            AATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTG                                               >  SRR4302533.4149528/1‑94 (MQ=60)

                                            AATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGCACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTG                                               >  SRR4302533.3010867/1‑94 (MQ=60)

                                               GACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGA                                            <  SRR4302533.2902636/94‑1 (MQ=60)

                                                ACNAAANCCGCTTCCACGCCGANNAGTGGGTAGGGTACAGCAAGGNGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAA                                           <  SRR4302533.4652142/94‑1 (MQ=60)

                                                ACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAA                                           >  SRR4302533.2789809/1‑94 (MQ=60)

                                                 CAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAA                                          >  SRR4302533.1638690/1‑94 (MQ=60)

                                                  AAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAC                                         >  SRR4302533.2474467/1‑94 (MQ=60)

                                                  AAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAAC                                         <  SRR4302533.818104/94‑1 (MQ=60)

                                                   AAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACG                                        <  SRR4302533.5074192/94‑1 (MQ=60)

                                                   AAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACG                                        <  SRR4302533.1737474/94‑1 (MQ=60)

                                                   AAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGACTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACG                                        >  SRR4302533.2443386/1‑94 (MQ=60)

                                                      CCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTT                                     >  SRR4302533.223915/1‑94 (MQ=60)

                                                      CCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTT                                     >  SRR4302533.5064373/1‑94 (MQ=60)

                                                       CCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTT                                    <  SRR4302533.958621/94‑1 (MQ=60)

                                                        CGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTC                                   >  SRR4302533.39677/1‑94 (MQ=60)

                                                         GCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCA                                  >  SRR4302533.5340515/1‑94 (MQ=60)

                                                         GCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCA                                  >  SRR4302533.2928932/1‑94 (MQ=60)

                                                          CTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAA                                 >  SRR4302533.3137968/1‑94 (MQ=60)

                                                          CTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAA                                 >  SRR4302533.5408168/1‑94 (MQ=60)

                                                           TTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAAT                                <  SRR4302533.2714164/94‑1 (MQ=60)

                                                            TCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATA                               <  SRR4302533.2064965/94‑1 (MQ=60)

                                                            TCCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATA                               <  SRR4302533.1081400/94‑1 (MQ=60)

                                                             CCACGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATAC                              >  SRR4302533.6260618/1‑94 (MQ=60)

                                                                CGCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTC                           <  SRR4302533.2256509/94‑1 (MQ=60)

                                                                 GCCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCC                          <  SRR4302533.161704/94‑1 (MQ=60)

                                                                  CCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCA                         >  SRR4302533.4045338/1‑94 (MQ=60)

                                                                  CCGACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCA                         >  SRR4302533.1219179/1‑94 (MQ=60)

                                                                     ACAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAAC                      <  SRR4302533.3285837/94‑1 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCACGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     <  SRR4302533.4228684/94‑1 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     >  SRR4302533.397357/1‑94 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     >  SRR4302533.1888528/1‑94 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     >  SRR4302533.1365820/1‑94 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     <  SRR4302533.3301713/94‑1 (MQ=60)

                                                                      CAAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACG                     <  SRR4302533.3651758/94‑1 (MQ=60)

                                                                       AAGTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGA                    >  SRR4302533.993854/1‑94 (MQ=60)

                                                                         GTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATG                  <  SRR4302533.4047778/94‑1 (MQ=60)

                                                                         GTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATG                  <  SRR4302533.3108617/94‑1 (MQ=60)

                                                                         GTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATG                  <  SRR4302533.5235464/94‑1 (MQ=60)

                                                                         GTGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATG                  <  SRR4302533.1589731/94‑1 (MQ=60)

                                                                          TGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGT                 >  SRR4302533.5468553/1‑94 (MQ=60)

                                                                          TGGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGT                 <  SRR4302533.4690765/94‑1 (MQ=60)

                                                                           GGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTA                <  SRR4302533.6055250/94‑1 (MQ=60)

                                                                           GGGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTA                <  SRR4302533.1280786/94‑1 (MQ=60)

                                                                            GGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTAC               <  SRR4302533.2661601/94‑1 (MQ=60)

                                                                            GGTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTAC               >  SRR4302533.667217/1‑94 (MQ=60)

                                                                             GTAGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACG              >  SRR4302533.618254/1‑94 (MQ=60)

                                                                               AGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGAC            >  SRR4302533.513672/1‑94 (MQ=60)

                                                                               AGGGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGAC            >  SRR4302533.4980930/1‑94 (MQ=60)

                                                                                 GGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACAT          >  SRR4302533.3528116/1‑94 (MQ=60)

                                                                                 GGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACAT          <  SRR4302533.2578424/94‑1 (MQ=60)

                                                                                 GGTACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACAT          >  SRR4302533.2885473/1‑94 (MQ=60)

                                                                                   TACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGG        >  SRR4302533.252099/1‑94 (MQ=60)

                                                                                   TACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGG        >  SRR4302533.1672263/1‑94 (MQ=60)

                                                                                   TACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGG        <  SRR4302533.5098128/94‑1 (MQ=60)

                                                                                    ACAGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGA       >  SRR4302533.4490612/1‑94 (MQ=60)

                                                                                      AGCAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAAC     <  SRR4302533.2626339/94‑1 (MQ=60)

                                                                                        CAAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGG   >  SRR4302533.2900225/1‑94 (MQ=60)

                                                                                         AAGGCGCTTGCCTGGGTGAGCTTATCGCACAAAGACCCCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGC  <  SRR4302533.4953516/94‑1 (MQ=60)

TGCATCCAGAATATGCGAAGCACTGGAACATGAAAGGCGTCATGAATGACAAAACCCGCTTCCACGCCGACAAGTGGGTAGGGTACAGCAAGGTGCTTGCCTGGGTGAGCTTATCGCACAAAGACACCTTCACGGGTGCGAAACGGTTTCAATACTTCCAAACGATGTACGACATGGAACGGC  >  NC_000913/2560794‑2560976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`