Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,561,679	A→G	Y28C (TAC→TGC)	b2448 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,561,679	0	A	G	99.6%	817.8 / ‑4.3	250	Y28C (TAC→TGC)	b2448	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (140/109); minor base T (0/1); total (140/110)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 254 total aligned reads displayed.

ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGC  >  NC_000913/2561589‑2561768

aTTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCa                                                                                         <  1:4628814/93‑1 (MQ=255)

aTTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCa                                                                                         <  2:5228391/93‑1 (MQ=255)

  tGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAgg                                                                                       <  1:3031087/93‑1 (MQ=255)

   gAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGc                                                                                      <  2:1982790/93‑1 (MQ=255)

    aaCTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCt                                                                                     >  1:3282660/1‑93 (MQ=255)

      cTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGa                                                                                   >  2:3987570/1‑93 (MQ=255)

       tgtgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTTTCGATGTTTGTGATTTGAGGCTGaa                                                                                  >  2:4294376/1‑93 (MQ=255)

        gtgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAc                                                                                 <  1:1576112/93‑1 (MQ=255)

        gtgAAGGAGGACGCCATGAAACTTTTAAGCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAc                                                                                 <  2:3472151/93‑1 (MQ=255)

         tgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACg                                                                                >  2:2100573/1‑93 (MQ=255)

          gAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACgg                                                                               <  1:136461/93‑1 (MQ=255)

             ggaggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACggggg                                                                            >  1:5197987/1‑92 (MQ=255)

               aggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggtgg                                                                          >  1:4588047/1‑93 (MQ=255)

               aggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagg                                                                          >  1:1922642/1‑93 (MQ=255)

                ggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagga                                                                         <  1:1014187/93‑1 (MQ=255)

                ggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagga                                                                         <  1:2710931/93‑1 (MQ=255)

                 gaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAt                                                                        >  2:2880313/1‑93 (MQ=255)

                 gaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAt                                                                        >  1:3456910/1‑93 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  >  1:883064/1‑93 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  <  2:5573660/93‑1 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACCGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  >  1:3658207/1‑93 (MQ=255)

                        tGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCaa                                                                 >  1:5030688/1‑93 (MQ=255)

                         gAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAg                                                                >  2:2420265/1‑93 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  2:2782535/93‑1 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  1:4897965/93‑1 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  1:554230/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:4763361/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:6037224/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              >  1:1562384/1‑93 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:2343271/93‑1 (MQ=255)

                             cTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAAtt                                                            >  2:5531376/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  2:3078871/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:1053605/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:409795/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:1172548/1‑93 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          >  2:424351/1‑93 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          <  2:2532242/93‑1 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          >  2:2442360/1‑93 (MQ=255)

                                ttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGaa                                                         <  1:4166931/93‑1 (MQ=255)

                                  aaTCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATc                                                       <  2:2018878/93‑1 (MQ=255)

                                  aaTCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATc                                                       <  1:653119/93‑1 (MQ=255)

                                     cGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGt                                                    <  2:5833065/93‑1 (MQ=255)

                                     cGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGt                                                    >  2:4535372/1‑93 (MQ=255)

                                      gCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTc                                                   <  1:1993284/93‑1 (MQ=255)

                                       cAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCg                                                  >  2:6152128/1‑93 (MQ=255)

                                       cAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCg                                                  >  2:4630085/1‑93 (MQ=255)

                                        aaTCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGt                                                 >  2:779135/1‑93 (MQ=255)

                                        aaTCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGt                                                 <  1:3572022/93‑1 (MQ=255)

                                         atcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGtt                                                >  1:3149361/1‑93 (MQ=255)

                                         atcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGtt                                                >  1:1249955/1‑93 (MQ=255)

                                          tcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTa                                               >  2:3001/1‑93 (MQ=255)

                                           catcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTAc                                              <  2:2287376/93‑1 (MQ=255)

                                           catcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTAc                                              >  1:2826389/1‑93 (MQ=255)

                                              ccTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGa                                           >  2:1020489/1‑93 (MQ=255)

                                               cTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGAt                                          >  1:3529608/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  1:1347673/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  2:6047958/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  1:2117419/1‑93 (MQ=255)

                                                 gATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATaa                                        >  2:4887885/1‑93 (MQ=255)

                                                  aTGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAAc                                       >  2:1100871/1‑93 (MQ=255)

                                                   tGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCACGAATTGAATCAGTCGTTACTGAtcaat                                      >  2:4333232/1‑89 (MQ=255)

                                                    ggTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtg                                     >  1:3844189/1‑93 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    <  1:4827185/93‑1 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    <  2:2642367/93‑1 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    >  2:2388104/1‑93 (MQ=255)

                                                         tGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaa                                >  1:5459739/1‑93 (MQ=255)

                                                          gACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACCGTGaaaa                               >  1:5627777/1‑93 (MQ=255)

                                                           aCTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaaaa                              >  2:2184342/1‑93 (MQ=255)

                                                            cTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaaaaa                             >  2:3647729/1‑93 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  1:2415522/93‑1 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  2:1443542/93‑1 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            >  1:160827/1‑93 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  1:3728173/93‑1 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  1:2074296/1‑93 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  1:4071605/1‑93 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  2:3294842/1‑93 (MQ=255)

                                                                tgtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGc                         >  1:1197178/1‑93 (MQ=255)

                                                                tgtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGc                         >  1:6176952/1‑93 (MQ=255)

                                                                 gtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGcc                        >  2:4573201/1‑93 (MQ=255)

                                                                 gtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGcc                        <  2:3907650/93‑1 (MQ=255)

                                                                  tttgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGCTACTGATAACTGTGAAAAAACAGCCg                       >  1:3357121/1‑93 (MQ=255)

                                                                   ttgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGt                      <  1:5688566/93‑1 (MQ=255)

                                                                    tgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTa                     <  1:5607650/93‑1 (MQ=255)

                                                                     gctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTAt                    >  2:6015544/1‑93 (MQ=255)

                                                                      ctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTAtt                   <  1:4425475/93‑1 (MQ=255)

                                                                        ttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTgg                 <  2:2869198/93‑1 (MQ=255)

                                                                         tgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGt                <  1:7108/93‑1 (MQ=255)

                                                                           cAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTCTTGGTAg              >  2:3683127/1‑93 (MQ=255)

                                                                           cAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAg              >  2:534782/1‑93 (MQ=255)

                                                                            aGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAgg             <  1:1399118/93‑1 (MQ=255)

                                                                            aGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAgg             >  2:339469/1‑93 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAggg            >  2:12425/1‑92 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGc            <  1:904842/93‑1 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGc            <  1:2219445/93‑1 (MQ=255)

                                                                               tGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCgg          >  2:5622292/1‑93 (MQ=255)

                                                                               tGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCgg          >  2:6259576/1‑93 (MQ=255)

                                                                                gTTGGTGATTGCAGGCTGAACTGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGt         >  1:5960481/1‑93 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  <  1:2235076/93‑1 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  >  1:3941549/1‑93 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  <  1:450758/93‑1 (MQ=255)

ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGC  >  NC_000913/2561589‑2561768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTT  >  NC_000913/2561586‑2561770

TGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTG                                                                                             <  SRR4302533.6316206/94‑1 (MQ=60)

  TATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCA                                                                                           <  SRR4302533.2510603/94‑1 (MQ=60)

   ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAG                                                                                          <  SRR4302533.4002720/94‑1 (MQ=60)

   ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAG                                                                                          <  SRR4302533.3530376/94‑1 (MQ=60)

   ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAG                                                                                          <  SRR4302533.4656866/94‑1 (MQ=60)

   ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAG                                                                                          <  SRR4302533.701772/94‑1 (MQ=60)

     TGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGC                                                                                        <  SRR4302533.3049636/94‑1 (MQ=60)

     TGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGC                                                                                        >  SRR4302533.884798/1‑94 (MQ=60)

      GAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCT                                                                                       >  SRR4302533.3302925/1‑94 (MQ=60)

      GAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCT                                                                                       >  SRR4302533.1368949/1‑94 (MQ=60)

       AACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTG                                                                                      <  SRR4302533.3646455/94‑1 (MQ=60)

         CTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAA                                                                                    >  SRR4302533.4843292/1‑94 (MQ=60)

          TGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAG                                                                                   >  SRR4302533.1584125/1‑94 (MQ=60)

          TGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAC                                                                                   <  SRR4302533.2318767/94‑1 (MQ=60)

          TGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAC                                                                                   <  SRR4302533.5975286/94‑1 (MQ=60)

          TGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAC                                                                                   <  SRR4302533.1199478/94‑1 (MQ=60)

           GTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACG                                                                                  <  SRR4302533.1585600/94‑1 (MQ=60)

           GTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACG                                                                                  >  SRR4302533.4677076/1‑94 (MQ=60)

           GTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACG                                                                                  <  SRR4302533.1610321/94‑1 (MQ=60)

           GTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACG                                                                                  >  SRR4302533.3325854/1‑94 (MQ=60)

            TGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGG                                                                                 >  SRR4302533.4143815/1‑94 (MQ=60)

             GAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGG                                                                                <  SRR4302533.1466294/94‑1 (MQ=60)

             GAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGG                                                                                <  SRR4302533.3090199/94‑1 (MQ=60)

             GAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGG                                                                                <  SRR4302533.137325/94‑1 (MQ=60)

               AGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGG                                                                              >  SRR4302533.5229334/1‑94 (MQ=60)

               AGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGG                                                                              >  SRR4302533.2018073/1‑94 (MQ=60)

                GGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAG                                                                             <  SRR4302533.2186232/94‑1 (MQ=60)

                GGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGAGAG                                                                             >  SRR4302533.2968867/1‑94 (MQ=60)

                 GAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGTGG                                                                            >  SRR4302533.4615837/1‑94 (MQ=60)

                 GAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGG                                                                            >  SRR4302533.1934376/1‑94 (MQ=60)

                  AGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGA                                                                           >  SRR4302533.4148704/1‑94 (MQ=60)

                   GGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAT                                                                          >  SRR4302533.3478253/1‑94 (MQ=60)

                   GGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAT                                                                          <  SRR4302533.2727572/94‑1 (MQ=60)

                   GGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAT                                                                          <  SRR4302533.1020127/94‑1 (MQ=60)

                    GACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATA                                                                         <  SRR4302533.2577645/94‑1 (MQ=60)

                     ACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAA                                                                        <  SRR4302533.564979/94‑1 (MQ=60)

                      CGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAAC                                                                       <  SRR4302533.4677777/94‑1 (MQ=60)

                      CGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAAC                                                                       >  SRR4302533.1221347/1‑94 (MQ=60)

                         CATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCA                                                                    >  SRR4302533.4970189/1‑94 (MQ=60)

                         CATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCA                                                                    >  SRR4302533.888251/1‑94 (MQ=60)

                         CATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCA                                                                    <  SRR4302533.2333891/94‑1 (MQ=60)

                         CATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCA                                                                    >  SRR4302533.4653011/1‑94 (MQ=60)

                         CATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACCGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCA                                                                    >  SRR4302533.3680701/1‑94 (MQ=60)

                          ATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAA                                                                   >  SRR4302533.5061148/1‑94 (MQ=60)

                          ATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAA                                                                   >  SRR4302533.4502757/1‑94 (MQ=60)

                          ATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAA                                                                   >  SRR4302533.1209687/1‑94 (MQ=60)

                            GAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGA                                                                 >  SRR4302533.112547/1‑94 (MQ=60)

                             AAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAA                                                                <  SRR4302533.557516/94‑1 (MQ=60)

                             AAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAA                                                                >  SRR4302533.1571788/1‑94 (MQ=60)

                             AAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAA                                                                <  SRR4302533.4927645/94‑1 (MQ=60)

                              AACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAAT                                                               <  SRR4302533.4792250/94‑1 (MQ=60)

                              AACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAAT                                                               <  SRR4302533.6073808/94‑1 (MQ=60)

                              AACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAAT                                                               <  SRR4302533.2357776/94‑1 (MQ=60)

                               ACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGCTGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATT                                                              <  SRR4302533.3398595/94‑1 (MQ=60)

                               ACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATT                                                              <  SRR4302533.90036/94‑1 (MQ=60)

                                CTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTG                                                             <  SRR4302533.3547489/94‑1 (MQ=60)

                                CTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTG                                                             >  SRR4302533.1059811/1‑94 (MQ=60)

                                CTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTG                                                             >  SRR4302533.4620432/1‑94 (MQ=60)

                                CTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTG                                                             >  SRR4302533.412252/1‑94 (MQ=60)

                                CTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTG                                                             >  SRR4302533.1179586/1‑94 (MQ=60)

                                  TTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAA                                                           <  SRR4302533.1426781/94‑1 (MQ=60)

                                  TTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAA                                                           <  SRR4302533.2400035/94‑1 (MQ=60)

                                  TGTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAA                                                           <  SRR4302533.3617200/94‑1 (MQ=60)

                                   TTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAAT                                                          <  SRR4302533.4192181/94‑1 (MQ=60)

                                    TAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATC                                                         >  SRR4302533.2624117/1‑94 (MQ=60)

                                     AATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCA                                                        <  SRR4302533.656975/94‑1 (MQ=60)

                                      ATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAG                                                       <  SRR4302533.5534036/94‑1 (MQ=60)

                                      ATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAG                                                       >  SRR4302533.804298/1‑94 (MQ=60)

                                      ATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAG                                                       <  SRR4302533.3784118/94‑1 (MQ=60)

                                      ATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAG                                                       >  SRR4302533.3468708/1‑94 (MQ=60)

                                      ATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAG                                                       >  SRR4302533.6049053/1‑94 (MQ=60)

                                       TCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGT                                                      >  SRR4302533.1617613/1‑94 (MQ=60)

                                        CGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTC                                                     <  SRR4302533.6286376/94‑1 (MQ=60)

                                         GCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCG                                                    >  SRR4302533.2109897/1‑94 (MQ=60)

                                         GCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCG                                                    <  SRR4302533.2005510/94‑1 (MQ=60)

                                         GCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCG                                                    >  SRR4302533.5794099/1‑94 (MQ=60)

                                         GCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCG                                                    >  SRR4302533.6302768/1‑94 (MQ=60)

                                           AATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTT                                                  >  SRR4302533.1257421/1‑94 (MQ=60)

                                           AATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTT                                                  <  SRR4302533.3594034/94‑1 (MQ=60)

                                           AATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTT                                                  >  SRR4302533.3168722/1‑94 (MQ=60)

                                           AATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTT                                                  <  SRR4302533.2833895/94‑1 (MQ=60)

                                             TCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTAC                                                >  SRR4302533.2843781/1‑94 (MQ=60)

                                              CATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACT                                               >  SRR4302533.5645098/1‑94 (MQ=60)

                                                 CCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGAT                                            >  SRR4302533.3551362/1‑94 (MQ=60)

                                                  CTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATA                                           >  SRR4302533.2130478/1‑94 (MQ=60)

                                                  CTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATA                                           >  SRR4302533.1355783/1‑94 (MQ=60)

                                                   TGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAA                                          <  SRR4302533.3011688/94‑1 (MQ=60)

                                                     ATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACT                                        <  SRR4302533.5538863/94‑1 (MQ=60)

                                                      TGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTG                                       >  SRR4302533.3867668/1‑94 (MQ=60)

                                                      TGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTG                                       <  SRR4302533.1390040/94‑1 (MQ=60)

                                                        GTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTTCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTG                                     <  SRR4302533.3288716/94‑1 (MQ=60)

                                                        GTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTG                                     <  SRR4302533.2074996/94‑1 (MQ=60)

                                                        GTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTG                                     <  SRR4302533.4856438/94‑1 (MQ=60)

                                                         TGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGA                                    <  SRR4302533.4925127/94‑1 (MQ=60)

                                                           CTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAA                                  >  SRR4302533.5492633/1‑94 (MQ=60)

                                                            TGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAA                                 >  SRR4302533.1287071/1‑94 (MQ=60)

                                                            TGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACCGTGAAAA                                 >  SRR4302533.5661802/1‑94 (MQ=60)

                                                             GACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAA                                >  SRR4302533.4495427/1‑94 (MQ=60)

                                                              ACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAA                               >  SRR4302533.4574391/1‑94 (MQ=60)

                                                               CTGGTGTTTGCTTTGCAGATGTTGGTGATTGTAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAC                              >  SRR4302533.6072668/1‑94 (MQ=60)

                                                               CTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCACGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAC                              >  SRR4302533.915849/1‑94 (MQ=60)

                                                               CTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATCACTGTGAAAAAAC                              >  SRR4302533.5141489/1‑94 (MQ=60)

                                                               CTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAC                              >  SRR4302533.919183/1‑94 (MQ=60)

                                                               CTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAC                              >  SRR4302533.161853/1‑94 (MQ=60)

                                                                TGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACA                             >  SRR4302533.4096392/1‑94 (MQ=60)

                                                                TGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACA                             >  SRR4302533.2087070/1‑94 (MQ=60)

                                                                TGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACA                             <  SRR4302533.2430444/94‑1 (MQ=60)

                                                                TGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACA                             <  SRR4302533.3751043/94‑1 (MQ=60)

                                                                TGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACA                             >  SRR4302533.5584782/1‑94 (MQ=60)

                                                                 GGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAG                            <  SRR4302533.612802/94‑1 (MQ=60)

                                                                  GTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGC                           >  SRR4302533.1204369/1‑94 (MQ=60)

                                                                  GTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGC                           >  SRR4302533.6214062/1‑94 (MQ=60)

                                                                  GTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGC                           <  SRR4302533.2143006/94‑1 (MQ=60)

                                                                   TGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCC                          >  SRR4302533.4101288/1‑94 (MQ=60)

                                                                   TGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCC                          <  SRR4302533.5103882/94‑1 (MQ=60)

                                                                    GTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCG                         <  SRR4302533.1041913/94‑1 (MQ=60)

                                                                    GTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGCTACTGATAACTGTGAAAAAACAGCCG                         >  SRR4302533.3377865/1‑94 (MQ=60)

                                                                      TTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTA                       <  SRR4302533.5722925/94‑1 (MQ=60)

                                                                      TTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTA                       >  SRR4302533.4859856/1‑94 (MQ=60)

                                                                       TGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTAT                      <  SRR4302533.5641534/94‑1 (MQ=60)

                                                                        GCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATT                     <  SRR4302533.1889776/94‑1 (MQ=60)

                                                                         CTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTG                    <  SRR4302533.4452224/94‑1 (MQ=60)

                                                                         CTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTG                    <  SRR4302533.195460/94‑1 (MQ=60)

                                                                           TTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGT                  >  SRR4302533.3172834/1‑94 (MQ=60)

                                                                           TTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGA                  >  SRR4302533.4043073/1‑94 (MQ=60)

                                                                            TGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTA                 <  SRR4302533.3590251/94‑1 (MQ=60)

                                                                            TGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTA                 <  SRR4302533.7170/94‑1 (MQ=60)

                                                                             GCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAG                <  SRR4302533.223923/94‑1 (MQ=60)

                                                                               AGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGC              <  SRR4302533.1407551/94‑1 (MQ=60)

                                                                               AGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGC              >  SRR4302533.3688059/1‑94 (MQ=60)

                                                                               AGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGC              <  SRR4302533.5320599/94‑1 (MQ=60)

                                                                                GATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCG             <  SRR4302533.910146/94‑1 (MQ=60)

                                                                                GATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCG             <  SRR4302533.2233147/94‑1 (MQ=60)

                                                                                GATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCG             >  SRR4302533.5713747/1‑94 (MQ=60)

                                                                                GATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCG             <  SRR4302533.1168974/94‑1 (MQ=60)

                                                                                  TGTTGGTGATTGCAGGCTGAACTGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGT           >  SRR4302533.5996540/1‑94 (MQ=60)

                                                                                   GTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTG          >  SRR4302533.380609/1‑94 (MQ=60)

                                                                                      GGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACA       >  SRR4302533.3869509/1‑94 (MQ=60)

                                                                                      GGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACA       <  SRR4302533.4368148/94‑1 (MQ=60)

                                                                                       GTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAG      <  SRR4302533.1367514/94‑1 (MQ=60)

                                                                                       GTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAG      >  SRR4302533.199234/1‑94 (MQ=60)

                                                                                         GATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGC    >  SRR4302533.3965598/1‑94 (MQ=60)

                                                                                          ATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCT   >  SRR4302533.3529935/1‑94 (MQ=60)

                                                                                          ATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCT   <  SRR4302533.2248868/94‑1 (MQ=60)

                                                                                          ATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCT   <  SRR4302533.453457/94‑1 (MQ=60)

                                                                                           TTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTT  <  SRR4302533.164382/94‑1 (MQ=60)

TGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTT  >  NC_000913/2561586‑2561770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`