Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,699	T→C	Q220R (CAG→CGG)	eutJ ←	EutJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,699	0	T	C	99.4%	541.2 / ‑3.7	172	coding (659/837 nt)	eutJ	EutJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/80); minor base . (1/0); total (92/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

Only 100 of 200 total aligned reads displayed.

ACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG  >  NC_000913/2567609‑2567788

cccTGTAACGCCGGGAATTGTTTGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgc                                                                                         <  1:2201400/92‑1 (MQ=255)

cccTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgc                                                                                         <  1:1079800/92‑1 (MQ=255)

aCCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgc                                                                                         >  1:5850638/1‑93 (MQ=255)

 ccTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgcc                                                                                        >  2:2724417/1‑93 (MQ=255)

  cTGTATGGCCGAGATTTGTTGGCAAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgccc                                                                                       <  2:4723187/93‑1 (MQ=255)

  cTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAAtgccccgccc                                                                                       >  1:175239/1‑93 (MQ=255)

   tGTACCGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATgccccgccct                                                                                      <  2:1308983/93‑1 (MQ=255)

   tGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATgccccgccct                                                                                      >  1:1973581/1‑93 (MQ=255)

    gTAACGCCGGGAATTGTTTGCGAAACAGTCCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCtt                                                                                     <  2:4387271/93‑1 (MQ=255)

        cGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAat                                                                                 <  1:3682867/93‑1 (MQ=255)

         gcggggAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAata                                                                                <  1:163978/90‑1 (MQ=255)

         gCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAata                                                                                <  1:1257637/93‑1 (MQ=255)

          ccGGGAATTGTTTGCGAAACAGTCCCGCCACGCCCGTTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAatat                                                                               <  2:3514434/93‑1 (MQ=255)

           cGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATg                                                                              <  2:3821341/93‑1 (MQ=255)

            gggAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGc                                                                             >  1:5874520/1‑93 (MQ=255)

            gggAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGc                                                                             >  2:2139333/1‑93 (MQ=255)

            gggAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGc                                                                             >  2:5524763/1‑93 (MQ=255)

            gggAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGc                                                                             <  1:5733246/93‑1 (MQ=255)

             ggAATTGTTTGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGcc                                                                            <  1:4102444/93‑1 (MQ=255)

              gAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCg                                                                           >  2:6175681/1‑93 (MQ=255)

               aaTTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCgg                                                                          >  2:4961613/1‑93 (MQ=255)

               aaTTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCgg                                                                          >  1:5997908/1‑93 (MQ=255)

               aaTTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCgg                                                                          >  2:3984194/1‑93 (MQ=255)

               aaTTGTTTGCAAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCgg                                                                          <  2:5898144/93‑1 (MQ=255)

                aTTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCggg                                                                         <  1:4931364/93‑1 (MQ=255)

                aTTGTTTGCAAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCggg                                                                         <  2:678173/93‑1 (MQ=255)

                 ttGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGc                                                                        <  2:2339063/93‑1 (MQ=255)

                 ttGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGc                                                                        >  1:4069432/1‑93 (MQ=255)

                   ttttGCGAAGCATTGCGCCCACGCCCGGTTGCATCCAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGa                                                                      <  1:1818249/92‑1 (MQ=255)

                   ttttGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGa                                                                      <  1:2046203/92‑1 (MQ=255)

                    tttGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAc                                                                     >  1:1028814/1‑93 (MQ=255)

                    tttGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAc                                                                     >  1:5412276/1‑93 (MQ=255)

                     ttGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACa                                                                    >  1:5269533/1‑93 (MQ=255)

                      tccGAAACATTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACaa                                                                   <  1:5784413/91‑1 (MQ=255)

                        caaaaCAGTCCCGCCACGCCCGTTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATg                                                                 <  1:5745932/91‑1 (MQ=255)

                        cGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATg                                                                 >  2:465932/1‑93 (MQ=255)

                         gAAACAGTTCCGCCACGCCCGGTGGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGt                                                                <  2:3921031/93‑1 (MQ=255)

                          aaaCAGTTCCCCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTc                                                               <  1:5244457/93‑1 (MQ=255)

                           aaCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTcc                                                              >  1:4421713/1‑93 (MQ=255)

                            aCAGTTCCCCCACGCACGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGGCCg                                                             <  1:4157103/93‑1 (MQ=255)

                             cAGTTCCTCCACGCCCGGGTGCATACAAAAGCCGCCCGCCAGCCATAAATCAGTAATGTCCCGCCCTTCAATATGCCGGGCGACAATGTCCGc                                                            >  1:2353539/1‑93 (MQ=255)

                               gtcccGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCa                                                          <  2:5788741/90‑1 (MQ=255)

                               gTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACCATGTCCGCCa                                                          >  2:2069130/1‑93 (MQ=255)

                               gTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCa                                                          >  2:5599556/1‑93 (MQ=255)

                               gTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCa                                                          >  2:543305/1‑93 (MQ=255)

                               gTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCa                                                          <  1:3476493/93‑1 (MQ=255)

                                tcccGCCACCCCCGGTTGCATAAAAGACCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAt                                                         <  2:5726050/91‑1 (MQ=255)

                                  ccGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAttt                                                       >  1:6309180/1‑93 (MQ=255)

                                    gCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAttttt                                                     >  2:5969054/1‑93 (MQ=255)

                                    gCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAttttt                                                     >  1:958782/1‑93 (MQ=255)

                                       aCGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCg                                                  >  1:5617181/1‑93 (MQ=255)

                                         gCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCCGGCGACAATGTCCGCCATTTTTTCGTa                                                >  1:4696152/1‑93 (MQ=255)

                                           cggtttGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTaca                                              <  2:4746873/89‑1 (MQ=255)

                                            cGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTacac                                             <  1:5583001/93‑1 (MQ=255)

                                            cGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTacac                                             >  2:948257/1‑93 (MQ=255)

                                               ttGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCgg                                          <  2:3053416/93‑1 (MQ=255)

                                                tGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGt                                         >  1:3379540/1‑93 (MQ=255)

                                                 gCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGtt                                        >  1:4328661/1‑93 (MQ=255)

                                                  cATACAAGAGCCCCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGttt                                       <  2:1254310/93‑1 (MQ=255)

                                                        agagCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGc                                 >  1:1495296/1‑93 (MQ=255)

                                                        agacCCCCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGc                                 <  1:4525389/93‑1 (MQ=255)

                                                         gagCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCa                                >  2:4518855/1‑93 (MQ=255)

                                                         gagCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCa                                <  1:5091013/93‑1 (MQ=255)

                                                           gccgccCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAgg                              <  1:6165407/93‑1 (MQ=255)

                                                           acagccccccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAgg                              <  1:3779408/90‑1 (MQ=255)

                                                              ccccgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCa                           <  2:1593572/92‑1 (MQ=255)

                                                                ccgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCaaa                         >  2:5245260/1‑93 (MQ=255)

                                                                ccgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCaaa                         <  2:1912862/93‑1 (MQ=255)

                                                                 cgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAt                        >  1:2284011/1‑93 (MQ=255)

                                                                 cgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAt                        >  1:3094094/1‑93 (MQ=255)

                                                                 cgccAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAt                        >  1:3263575/1‑93 (MQ=255)

                                                                  cccagccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAtc                       <  2:5945287/92‑1 (MQ=255)

                                                                  cccagccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAtc                       <  2:474344/92‑1 (MQ=255)

                                                                   ccagccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAtct                      >  2:6044940/1‑93 (MQ=255)

                                                                     agccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATctct                    >  2:1479370/1‑93 (MQ=255)

                                                                     agccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATctct                    >  2:2861182/1‑93 (MQ=255)

                                                                      gccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCtt                   <  1:5325242/93‑1 (MQ=255)

                                                                       ccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTc                  <  2:3654150/93‑1 (MQ=255)

                                                                       ccaTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTc                  <  1:2832253/93‑1 (MQ=255)

                                                                        caTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCa                 >  2:635529/1‑93 (MQ=255)

                                                                        caTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCa                 >  2:3413632/1‑93 (MQ=255)

                                                                        caTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCa                 <  2:1057642/93‑1 (MQ=255)

                                                                        caTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCa                 <  1:1334971/93‑1 (MQ=255)

                                                                         aTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAc                >  1:797711/1‑93 (MQ=255)

                                                                         aTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAc                >  2:5282209/1‑93 (MQ=255)

                                                                         aTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAc                >  1:5304748/1‑93 (MQ=255)

                                                                         aTAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAc                >  1:999776/1‑93 (MQ=255)

                                                                            actCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTCCACCGGTTTCACCGCAGGCCAAATCTCTTCACCGt             <  2:2449207/91‑1 (MQ=255)

                                                                            aaTCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGt             >  2:2652255/1‑93 (MQ=255)

                                                                            aaTCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGt             >  2:5763016/1‑93 (MQ=255)

                                                                               cAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGAc          <  1:3557395/93‑1 (MQ=255)

                                                                                  tAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACcgc       <  2:3095078/93‑1 (MQ=255)

                                                                                   aaTGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACcgcg      >  1:2654098/1‑93 (MQ=255)

                                                                                     tgccccgcccTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCt    <  1:33395/93‑1 (MQ=255)

                                                                                      gccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCtt   >  1:2708054/1‑93 (MQ=255)

                                                                                      gccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCtt   >  1:2921054/1‑93 (MQ=255)

                                                                                      gccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCtt   <  1:1407656/93‑1 (MQ=255)

                                                                                      gccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCtt   <  2:879298/93‑1 (MQ=255)

                                                                                      gccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCtt   >  1:686216/1‑93 (MQ=255)

                                                                                       ccccgccctTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTg  >  1:3885112/1‑93 (MQ=255)

ACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG  >  NC_000913/2567609‑2567788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  >  NC_000913/2567606‑2567792

TGCACCTGTAACGCCGGAAATTGTTGGCGAAACAGTCCCCCCCCGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCC                                                                                               <  SRR4302533.4108639/94‑1 (MQ=60)

 GCACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCG                                                                                              >  SRR4302533.2167981/1‑94 (MQ=60)

  CACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGC                                                                                             >  SRR4302533.5886011/1‑94 (MQ=60)

   CCCTGTAACGCCGGGAATTGTTTGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCC                                                                                            <  SRR4302533.2214963/94‑1 (MQ=60)

   CCCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCC                                                                                            <  SRR4302533.1086163/94‑1 (MQ=60)

   ACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCC                                                                                            >  SRR4302533.5524921/1‑94 (MQ=60)

   ACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCC                                                                                            >  SRR4302533.6302812/1‑94 (MQ=60)

    caggtagcgccgggattcttttgcaaagcagtcCCGCCACGCCCGATTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCC                                                                                           <  SRR4302533.2360055/61‑1 (MQ=60)

    CCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCC                                                                                           >  SRR4302533.176344/1‑94 (MQ=60)

     CTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCT                                                                                          >  SRR4302533.1985664/1‑94 (MQ=60)

      TGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTT                                                                                         <  SRR4302533.1271263/94‑1 (MQ=60)

        TAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCA                                                                                       >  SRR4302533.2674832/1‑94 (MQ=60)

          ACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAAT                                                                                     >  SRR4302533.4299230/1‑94 (MQ=60)

           CGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATA                                                                                    <  SRR4302533.3705503/94‑1 (MQ=60)

            GCGGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATAT                                                                                   <  SRR4302533.165021/94‑1 (MQ=60)

            GCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATAT                                                                                   <  SRR4302533.1265156/94‑1 (MQ=60)

             CCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATG                                                                                  >  SRR4302533.4361502/1‑94 (MQ=60)

             CCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATG                                                                                  <  SRR4302533.5354479/94‑1 (MQ=60)

              CGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGC                                                                                 >  SRR4302533.5910029/1‑94 (MQ=60)

               GGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCC                                                                                <  SRR4302533.5767859/94‑1 (MQ=60)

                GGAATTGTTTGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCG                                                                               <  SRR4302533.4127406/94‑1 (MQ=60)

                 GAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGG                                                                              >  SRR4302533.1645414/1‑94 (MQ=60)

                 GAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGG                                                                              >  SRR4302533.6034235/1‑94 (MQ=60)

                  AATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGTCCTTCAATATGCCGGG                                                                             <  SRR4302533.1835095/94‑1 (MQ=60)

                  AATTGTTTGCGAAACAGTCCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGG                                                                             <  SRR4302533.1690332/94‑1 (MQ=60)

                   ATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGC                                                                            <  SRR4302533.4961225/94‑1 (MQ=60)

                   ATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGC                                                                            >  SRR4302533.4094205/1‑94 (MQ=60)

                   ATTGTTGGCGAAACAGTTCCGCCACGCCCGTTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGC                                                                            <  SRR4302533.2851241/94‑1 (MQ=60)

                    TTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCG                                                                           >  SRR4302533.4344086/1‑94 (MQ=60)

                      ttttgcgaagcattgcgcCCACGCCCGGTTGCATCCAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAC                                                                         <  SRR4302533.1829355/76‑1 (MQ=60)

                      gttgcggaaacagtgccgcagcgcctGGTTGCATACAAGACCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAC                                                                         <  SRR4302533.4158446/68‑1 (MQ=60)

                      TTTTGCGAACCAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAC                                                                         <  SRR4302533.2058821/94‑1 (MQ=60)

                      GTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAC                                                                         >  SRR4302533.5444820/1‑94 (MQ=60)

                      GTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGAC                                                                         >  SRR4302533.1034855/1‑94 (MQ=60)

                       TTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACA                                                                        >  SRR4302533.5301139/1‑94 (MQ=60)

                         TGCGAAACAGTCCCGCCACGCCGGGTTGCATACAAGACCCCCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAAT                                                                      <  SRR4302533.2034270/94‑1 (MQ=60)

                         TCCGAAACATTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAAT                                                                      <  SRR4302533.5819345/94‑1 (MQ=60)

                          gggaaacgttcCCGCCACGCCCGTTTGCAGTCAAGGGCCGCCGCCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATG                                                                     <  SRR4302533.1404017/83‑1 (MQ=57)

                           CAAAACGGTTCCGCCACGCCCGGTTGCATCCAAGACCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGT                                                                    <  SRR4302533.5632142/94‑1 (MQ=60)

                           CAAAACAGTCCCGCCACGCCCGTTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGT                                                                    <  SRR4302533.5780605/94‑1 (MQ=60)

                             AACCAGTTCCGCCACGCCGGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCC                                                                  <  SRR4302533.3381331/94‑1 (MQ=60)

                             AAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCC                                                                  >  SRR4302533.4448448/1‑94 (MQ=60)

                             AAACAGTTCCCCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCC                                                                  <  SRR4302533.5275979/94‑1 (MQ=60)

                               ACAGTTCCTCCACGCCCGGGTGCATACAAAAGCCGCCCGCCAGCCATAAATCAGTAATGTCCCGCCCTTCAATATGCCGGGCGACAATGTCCGC                                                                >  SRR4302533.2368094/1‑94 (MQ=60)

                               ACAGTTCCCCCACGCACGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGGCCGC                                                                <  SRR4302533.4182292/94‑1 (MQ=60)

                                CAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCC                                                               >  SRR4302533.341497/1‑94 (MQ=60)

                                 AGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTGCGCAA                                                              >  SRR4302533.4955924/1‑94 (MQ=60)

                                 AGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCA                                                              >  SRR4302533.1353464/1‑94 (MQ=60)

                                 AGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCA                                                              <  SRR4302533.935037/94‑1 (MQ=60)

                                  gcgccgcctcccccggttgcacaaaacacCCGCCTGCCATCCATAAATCAGTAACTCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAT                                                             <  SRR4302533.3497945/65‑1 (MQ=47)

                                  GTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCAT                                                             <  SRR4302533.3497944/94‑1 (MQ=60)

                                    TCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTT                                                           >  SRR4302533.6347104/1‑94 (MQ=60)

                                      CGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTT                                                         >  SRR4302533.964420/1‑94 (MQ=60)

                                         CACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCG                                                      >  SRR4302533.5651136/1‑94 (MQ=60)

                                           CGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCCGGCGACAATGTCCGCCATTTTTTCGTA                                                    >  SRR4302533.4724616/1‑94 (MQ=60)

                                             CCGGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACA                                                  <  SRR4302533.3051308/94‑1 (MQ=60)

                                             CCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACA                                                  >  SRR4302533.5913281/1‑94 (MQ=60)

                                              CCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACAC                                                 >  SRR4302533.3344258/1‑94 (MQ=60)

                                              CCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACAC                                                 >  SRR4302533.2133034/1‑94 (MQ=60)

                                              CCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACAC                                                 >  SRR4302533.5796206/1‑94 (MQ=60)

                                               CGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACC                                                <  SRR4302533.5616735/94‑1 (MQ=60)

                                                  TTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGT                                             >  SRR4302533.3400402/1‑94 (MQ=60)

                                                   TGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTT                                            >  SRR4302533.4354739/1‑94 (MQ=60)

                                                    TCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTT                                           <  SRR4302533.5021954/94‑1 (MQ=60)

                                                     CATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTC                                          <  SRR4302533.282181/94‑1 (MQ=60)

                                                      ATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCA                                         >  SRR4302533.642104/1‑94 (MQ=60)

                                                      ATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCA                                         >  SRR4302533.4612110/1‑94 (MQ=60)

                                                         CAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCG                                      >  SRR4302533.980118/1‑94 (MQ=60)

                                                          AAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGC                                     >  SRR4302533.1504306/1‑94 (MQ=60)

                                                           AGACCCCCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCA                                    <  SRR4302533.4552785/94‑1 (MQ=60)

                                                            GAGCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAG                                   <  SRR4302533.5121772/94‑1 (MQ=60)

                                                            GAGCCCCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAG                                   <  SRR4302533.451466/94‑1 (MQ=60)

                                                              acagccccCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGC                                 <  SRR4302533.3802561/86‑1 (MQ=60)

                                                              GCCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGC                                 <  SRR4302533.6202468/94‑1 (MQ=60)

                                                               CCGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCC                                >  SRR4302533.5277377/1‑94 (MQ=60)

                                                                CGCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCA                               >  SRR4302533.5856207/1‑94 (MQ=60)

                                                                 cccccCCAGCCATAAATCAGTAATGCGCTGCCCTTCAATATGCCGGGCTACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAA                              <  SRR4302533.3218114/89‑1 (MQ=60)

                                                                 GCCCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAA                              >  SRR4302533.3876212/1‑94 (MQ=60)

                                                                   CCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAT                            >  SRR4302533.2298149/1‑94 (MQ=60)

                                                                   CCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAT                            >  SRR4302533.1081331/1‑94 (MQ=60)

                                                                   CCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAT                            >  SRR4302533.3283706/1‑94 (MQ=60)

                                                                   CCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAT                            >  SRR4302533.3113117/1‑94 (MQ=60)

                                                                   CCGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAAT                            <  SRR4302533.5398278/94‑1 (MQ=60)

                                                                    CGCCAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGGTTCACCGCAGGCCAAATC                           >  SRR4302533.1140425/1‑94 (MQ=60)

                                                                       CAGCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCT                        >  SRR4302533.245863/1‑94 (MQ=60)

                                                                        AGCCATAAATCAGTAATGCCC‑GCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTC                      >  SRR4302533.5678870/1‑94 (MQ=60)

                                                                         GCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTC                      >  SRR4302533.5899361/1‑94 (MQ=60)

                                                                         GCCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTC                      <  SRR4302533.5357155/94‑1 (MQ=60)

                                                                          CCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCA                     >  SRR4302533.3790716/1‑94 (MQ=60)

                                                                          CCATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCA                     <  SRR4302533.2849689/94‑1 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    >  SRR4302533.1005611/1‑94 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    <  SRR4302533.1342981/94‑1 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    >  SRR4302533.5336535/1‑94 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    >  SRR4302533.5247304/1‑94 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    >  SRR4302533.802405/1‑94 (MQ=60)

                                                                           CATAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCAC                    >  SRR4302533.364267/1‑94 (MQ=60)

                                                                             TAAATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCG                  <  SRR4302533.2323972/94‑1 (MQ=60)

                                                                               AATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTG                >  SRR4302533.5777396/1‑94 (MQ=60)

                                                                               AATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTG                <  SRR4302533.2231164/94‑1 (MQ=60)

                                                                               AATCAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTG                <  SRR4302533.1821773/94‑1 (MQ=60)

                                                                                  CAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACC             <  SRR4302533.4096934/94‑1 (MQ=60)

                                                                                  CAGTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACC             <  SRR4302533.3579316/94‑1 (MQ=60)

                                                                                    GTAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGC           >  SRR4302533.5301393/1‑94 (MQ=60)

                                                                                     TAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCG          >  SRR4302533.2670400/1‑94 (MQ=60)

                                                                                     TAATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCG          <  SRR4302533.3958512/94‑1 (MQ=60)

                                                                                      AATGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGC         >  SRR4302533.6178383/1‑94 (MQ=60)

                                                                                        TGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTT       >  SRR4302533.2938947/1‑94 (MQ=60)

                                                                                        TGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTT       <  SRR4302533.33622/94‑1 (MQ=60)

                                                                                        TGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTT       >  SRR4302533.690274/1‑94 (MQ=60)

                                                                                        TGCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTT       >  SRR4302533.2724669/1‑94 (MQ=60)

                                                                                         GCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG      <  SRR4302533.1416167/94‑1 (MQ=60)

                                                                                         GCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG      <  SRR4302533.743045/94‑1 (MQ=60)

                                                                                         GCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG      <  SRR4302533.1424410/94‑1 (MQ=60)

                                                                                         GCCCCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTG      >  SRR4302533.3908816/1‑94 (MQ=60)

                                                                                            CCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTAC   <  SRR4302533.5522332/94‑1 (MQ=60)

                                                                                            CCGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTAC   <  SRR4302533.1268943/94‑1 (MQ=60)

                                                                                             CGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  <  SRR4302533.6233337/94‑1 (MQ=60)

                                                                                             CGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  <  SRR4302533.2201200/94‑1 (MQ=60)

                                                                                             CGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  <  SRR4302533.5404441/94‑1 (MQ=60)

                                                                                             CGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  >  SRR4302533.598452/1‑94 (MQ=60)

                                                                                             CGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  >  SRR4302533.1060575/1‑94 (MQ=60)

TGCACCTGTAACGCCGGGAATTGTTTGCGAAACAGTTCCGCCACGCCCGGTTGCATACAAGAGCCGCCCGCCAGCCATAAATCAGTAATGCCCTGCCCTTCAATATGCCGGGCGACAATGTCCGCCATTTTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACT  >  NC_000913/2567606‑2567792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`