Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,298 T→C T1004A (ACG→GCG)  b2520 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,2980TC100.0% 746.0 / NA 232T1004A (ACG→GCG) b2520hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (121/111);  total (121/111)

Only 100 of 244 total aligned reads displayed.

CACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC  >  NC_000913/2647208‑2647386
                                                                                          |                                                                                        
cACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGccc                                                                                        <  2:854168/93‑1 (MQ=255)
cACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGccc                                                                                        <  2:6187222/93‑1 (MQ=255)
 ccTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCa                                                                                       <  1:1579487/92‑1 (MQ=255)
 ccTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCa                                                                                       <  2:3393403/92‑1 (MQ=255)
 aCTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCa                                                                                       >  2:5157652/1‑93 (MQ=255)
 aCTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCa                                                                                       <  1:3348831/93‑1 (MQ=255)
    cGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGc                                                                                    <  2:5384580/93‑1 (MQ=255)
      ccATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCtt                                                                                  <  1:4670731/93‑1 (MQ=255)
       cATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTg                                                                                 >  2:4311390/1‑93 (MQ=255)
       cATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTg                                                                                 >  2:1435098/1‑93 (MQ=255)
         tAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTgcg                                                                               >  2:4447228/1‑93 (MQ=255)
           aaGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGcc                                                                             >  1:1135646/1‑93 (MQ=255)
               gcgGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGa                                                                         <  2:647840/93‑1 (MQ=255)
               gcgGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGa                                                                         <  2:5270093/93‑1 (MQ=255)
               gcgGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGa                                                                         >  2:1886988/1‑93 (MQ=255)
                 ggCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGAcg                                                                       >  2:2164719/1‑93 (MQ=255)
                  gCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGAcgc                                                                      <  2:2660528/93‑1 (MQ=255)
                    aTGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACgcgc                                                                    >  2:4501751/1‑93 (MQ=255)
                    aTGTTCAGTGCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACgcgc                                                                    <  1:2816893/93‑1 (MQ=255)
                     tGTTCAGTTCAGCAATCACCGGTGCGGCCACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCa                                                                   >  1:2965466/1‑93 (MQ=255)
                     tGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCa                                                                   >  1:6288759/1‑93 (MQ=255)
                     tGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCa                                                                   >  1:2094764/1‑93 (MQ=255)
                       ttcagttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAAt                                                                 <  1:4855324/93‑1 (MQ=255)
                        tcagttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAAtt                                                                <  2:3090926/93‑1 (MQ=255)
                           gttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAc                                                             <  2:4125175/93‑1 (MQ=255)
                           gttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAc                                                             <  2:3864476/93‑1 (MQ=255)
                           gttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAc                                                             >  1:2776101/1‑93 (MQ=255)
                            ttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAcc                                                            <  2:5830919/93‑1 (MQ=255)
                            ttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAcc                                                            <  2:3520601/93‑1 (MQ=255)
                                  aaTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaaa                                                      >  1:3849081/1‑93 (MQ=255)
                                  aaTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaaa                                                      >  1:5284649/1‑93 (MQ=255)
                                   aTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaaaa                                                     <  1:4222503/93‑1 (MQ=255)
                                   aTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaaaa                                                     >  1:5829530/1‑93 (MQ=255)
                                    tCACCGGTGCGGCGGCTATCACTCTACTTTCGTTGCTACCTAAGTCATCTGCCGCCCGGGCTTGCGCCATGACGCGCAATTCACCGTTAAAAt                                                    <  1:789733/93‑1 (MQ=255)
                                     cACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATc                                                   <  1:2759344/93‑1 (MQ=255)
                                     cACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGAGCCATGACGCGCAATTCACAGTTAAAATc                                                   >  2:4776919/1‑93 (MQ=255)
                                      aCCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCg                                                  >  1:1801421/1‑93 (MQ=255)
                                       ccGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGc                                                 <  2:2757953/93‑1 (MQ=255)
                                          gTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCaa                                              >  1:1230158/1‑93 (MQ=255)
                                          gTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCaa                                              >  2:2228743/1‑93 (MQ=255)
                                           tGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAAt                                             <  1:5515013/93‑1 (MQ=255)
                                           tGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAAt                                             <  1:2299188/93‑1 (MQ=255)
                                           tGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAAt                                             <  2:4465180/93‑1 (MQ=255)
                                           tGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAAt                                             <  2:100486/93‑1 (MQ=255)
                                            gCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATc                                            <  1:5591045/93‑1 (MQ=255)
                                              ggCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCgg                                          <  1:5694638/93‑1 (MQ=255)
                                              ggCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCgg                                          <  1:60180/93‑1 (MQ=255)
                                               gCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGc                                         >  1:5182471/1‑93 (MQ=255)
                                                cAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCa                                        <  2:6018671/93‑1 (MQ=255)
                                                cAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCa                                        <  2:3107417/93‑1 (MQ=255)
                                                 aaCTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAg                                       >  2:5465520/1‑93 (MQ=255)
                                                 aaCTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAg                                       >  2:2935493/1‑93 (MQ=255)
                                                  aCTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgt                                      >  2:5311422/1‑93 (MQ=255)
                                                  aCTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgt                                      <  1:34569/93‑1 (MQ=255)
                                                   cTATCACTTTACTTTCGTTGCTACCGAAGTCATCTCCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGCTAAAATCGCCAATCGGCActg                                     >  2:1578671/1‑93 (MQ=255)
                                                    tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt                                    <  2:2351228/93‑1 (MQ=255)
                                                    tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt                                    <  2:3320302/93‑1 (MQ=255)
                                                    tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt                                    >  2:4107229/1‑93 (MQ=255)
                                                    tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt                                    <  1:4769885/93‑1 (MQ=255)
                                                    tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt                                    <  1:4424771/93‑1 (MQ=255)
                                                     aTCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTa                                   >  2:1183564/1‑93 (MQ=255)
                                                     aTCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTa                                   >  2:3969888/1‑93 (MQ=255)
                                                     aTCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTa                                   <  2:1273680/93‑1 (MQ=255)
                                                      tCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTaa                                  >  2:1103547/1‑93 (MQ=255)
                                                       cACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAc                                 <  2:2481089/93‑1 (MQ=255)
                                                       cACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAc                                 <  1:5118024/93‑1 (MQ=255)
                                                       cACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAc                                 <  2:1292402/93‑1 (MQ=255)
                                                       cACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAc                                 <  2:3668010/93‑1 (MQ=255)
                                                        actttactttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAcc                                >  1:5254431/1‑93 (MQ=255)
                                                        actttactttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAcc                                <  2:4033704/93‑1 (MQ=255)
                                                         ctttactttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCg                               >  1:5866672/1‑93 (MQ=255)
                                                         ctttactttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCg                               <  1:3412552/93‑1 (MQ=255)
                                                           ttactttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAg                             >  1:2342704/1‑93 (MQ=255)
                                                             actttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGcc                           >  2:3422281/1‑93 (MQ=255)
                                                             actttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGcc                           >  1:2182155/1‑93 (MQ=255)
                                                               tttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCtt                         >  1:3211366/1‑93 (MQ=255)
                                                               tttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCtt                         >  2:1413897/1‑93 (MQ=255)
                                                               tttCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCtt                         <  1:924510/93‑1 (MQ=255)
                                                                  cGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGc                      >  1:5551245/1‑93 (MQ=255)
                                                                   gTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGcc                     >  1:4317526/1‑93 (MQ=255)
                                                                     tGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCt                   <  2:5056242/93‑1 (MQ=255)
                                                                     tGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCt                   <  1:1043176/93‑1 (MQ=255)
                                                                        tACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGtt                >  1:388649/1‑93 (MQ=255)
                                                                         aCCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTc               >  2:472884/1‑93 (MQ=255)
                                                                          ccGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCg              >  1:1927138/1‑93 (MQ=255)
                                                                          ccGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCg              >  1:1435665/1‑93 (MQ=255)
                                                                           cGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGt             >  1:2518740/1‑93 (MQ=255)
                                                                            gAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt            <  1:1641731/93‑1 (MQ=255)
                                                                            gAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt            >  2:1798302/1‑93 (MQ=255)
                                                                            gAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt            <  1:446048/93‑1 (MQ=255)
                                                                            gAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt            >  1:1410586/1‑93 (MQ=255)
                                                                            gAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt            <  2:564714/93‑1 (MQ=255)
                                                                             aaGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTg           >  1:2851166/1‑93 (MQ=255)
                                                                                 cATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCg       >  2:420991/1‑93 (MQ=255)
                                                                                   tCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGtt     >  1:297710/1‑93 (MQ=255)
                                                                                    cTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTa    <  2:5949634/93‑1 (MQ=255)
                                                                                     tGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAc   <  1:3621132/93‑1 (MQ=255)
                                                                                     tGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAc   >  1:6214685/1‑93 (MQ=255)
                                                                                     tGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAc   <  2:3289106/93‑1 (MQ=255)
                                                                                     tGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAc   >  1:2378580/1‑93 (MQ=255)
                                                                                          |                                                                                        
CACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC  >  NC_000913/2647208‑2647386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCA  >  NC_000913/2647205‑2647390
                                                                                             |                                                                                            
CGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACAGGTGCGGCGACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGC                                                                                              >  SRR4302533.3399312/1‑94 (MQ=60)
   CACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCA                                                                                           >  SRR4302533.5297955/1‑94 (MQ=60)
    CCTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAG                                                                                          <  SRR4302533.1589002/94‑1 (MQ=60)
    ACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAG                                                                                          >  SRR4302533.3869976/1‑94 (MQ=60)
    ACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAG                                                                                          <  SRR4302533.3369522/94‑1 (MQ=60)
    ACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAG                                                                                          >  SRR4302533.246146/1‑94 (MQ=60)
    ACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAG                                                                                          >  SRR4302533.1436653/1‑94 (MQ=60)
     CTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGG                                                                                         >  SRR4302533.5812031/1‑94 (MQ=60)
       CGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCT                                                                                       >  SRR4302533.5216785/1‑94 (MQ=60)
         CCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTG                                                                                     >  SRR4302533.177540/1‑94 (MQ=60)
         CCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTG                                                                                     >  SRR4302533.541811/1‑94 (MQ=60)
         CCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTG                                                                                     <  SRR4302533.4699035/94‑1 (MQ=60)
           ATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCG                                                                                   <  SRR4302533.1452419/94‑1 (MQ=60)
             AAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCC                                                                                 >  SRR4302533.1142400/1‑94 (MQ=60)
             AAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCC                                                                                 <  SRR4302533.4074261/94‑1 (MQ=60)
               AGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCAT                                                                               <  SRR4302533.5940285/94‑1 (MQ=60)
                   CGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACG                                                                           >  SRR4302533.2113602/1‑94 (MQ=60)
                      CATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGC                                                                        <  SRR4302533.1627683/94‑1 (MQ=60)
                      CATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGC                                                                        <  SRR4302533.4478877/94‑1 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCCACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.2983590/1‑94 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.2984301/1‑94 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.3471824/1‑94 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.2107662/1‑94 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.505345/1‑94 (MQ=60)
                       ATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       >  SRR4302533.6326271/1‑94 (MQ=60)
                       ATGTTCAGTGCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCA                                                                       <  SRR4302533.2834233/94‑1 (MQ=60)
                        TGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAA                                                                      >  SRR4302533.327264/1‑94 (MQ=60)
                         GTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAAT                                                                     >  SRR4302533.4713952/1‑94 (MQ=60)
                         GTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAAT                                                                     >  SRR4302533.2352563/1‑94 (MQ=60)
                          TTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATT                                                                    <  SRR4302533.4884748/94‑1 (MQ=60)
                             AGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCAC                                                                 >  SRR4302533.2793152/1‑94 (MQ=60)
                              GTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACC                                                                >  SRR4302533.900070/1‑94 (MQ=60)
                                   GCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAA                                                           <  SRR4302533.4649667/94‑1 (MQ=60)
                                    CAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAA                                                          >  SRR4302533.5316305/1‑94 (MQ=60)
                                    CAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAA                                                          >  SRR4302533.3872585/1‑94 (MQ=60)
                                     AATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAA                                                         >  SRR4302533.5864758/1‑94 (MQ=60)
                                     AATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAA                                                         <  SRR4302533.1458054/94‑1 (MQ=60)
                                     AATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAA                                                         <  SRR4302533.2144217/94‑1 (MQ=60)
                                      ATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAAT                                                        <  SRR4302533.4248032/94‑1 (MQ=60)
                                      ATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAAT                                                        <  SRR4302533.5724037/94‑1 (MQ=60)
                                      ATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGAAGCGCAATTCACCGTTAAAAT                                                        >  SRR4302533.4839696/1‑94 (MQ=60)
                                       TCACCGGTGCGGCGGCTATCACTCTACTTTCGTTGCTACCTAAGTCATCTGCCGCCCGGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATC                                                       <  SRR4302533.794380/94‑1 (MQ=60)
                                        CACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCG                                                      >  SRR4302533.3033401/1‑94 (MQ=60)
                                        CACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCG                                                      <  SRR4302533.2776257/94‑1 (MQ=60)
                                        CACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCG                                                      >  SRR4302533.1812384/1‑94 (MQ=60)
                                         cccgtgGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGC                                                     <  SRR4302533.3053635/88‑1 (MQ=60)
                                         ACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGC                                                     <  SRR4302533.6168869/94‑1 (MQ=60)
                                         ACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGC                                                     >  SRR4302533.2964679/1‑94 (MQ=60)
                                            GGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAA                                                  >  SRR4302533.2260893/1‑94 (MQ=60)
                                            GGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAA                                                  >  SRR4302533.1237521/1‑94 (MQ=60)
                                              TGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATC                                                <  SRR4302533.2313443/94‑1 (MQ=60)
                                              TGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATC                                                <  SRR4302533.5548309/94‑1 (MQ=60)
                                              TGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATC                                                <  SRR4302533.506761/94‑1 (MQ=60)
                                               GCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTCAAATCGCCAATCG                                               >  SRR4302533.785808/1‑94 (MQ=60)
                                               GCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCG                                               <  SRR4302533.5624818/94‑1 (MQ=60)
                                                CGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGG                                              <  SRR4302533.2048185/94‑1 (MQ=60)
                                                 GGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGC                                             >  SRR4302533.5213762/1‑94 (MQ=60)
                                                 GGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGC                                             <  SRR4302533.60572/94‑1 (MQ=60)
                                                 GGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGC                                             <  SRR4302533.5729028/94‑1 (MQ=60)
                                                   CAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAG                                           >  SRR4302533.5188858/1‑94 (MQ=60)
                                                    AACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGT                                          >  SRR4302533.251261/1‑94 (MQ=60)
                                                     ACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTG                                         <  SRR4302533.34801/94‑1 (MQ=60)
                                                     ACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTG                                         >  SRR4302533.5534925/1‑94 (MQ=60)
                                                      CTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCTCAGGCTTGCTCCATGATGCGCAATTCACCGTTAAAATCGCCAATCGCCAGTGT                                        >  SRR4302533.1073695/1‑94 (MQ=60)
                                                      CTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGT                                        >  SRR4302533.3904109/1‑94 (MQ=60)
                                                       TATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTA                                       <  SRR4302533.4451518/94‑1 (MQ=60)
                                                       TATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTA                                       <  SRR4302533.4798796/94‑1 (MQ=60)
                                                        ATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGTCAGTGTAA                                      >  SRR4302533.189073/1‑94 (MQ=60)
                                                          CACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACC                                    <  SRR4302533.452373/94‑1 (MQ=60)
                                                          CACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACC                                    >  SRR4302533.5285997/1‑94 (MQ=60)
                                                          CACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACC                                    >  SRR4302533.5882416/1‑94 (MQ=60)
                                                          CACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACC                                    <  SRR4302533.5148948/94‑1 (MQ=60)
                                                           ACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCG                                   >  SRR4302533.5902135/1‑94 (MQ=60)
                                                            CTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGA                                  >  SRR4302533.2795278/1‑94 (MQ=60)
                                                            CTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGA                                  >  SRR4302533.648657/1‑94 (MQ=60)
                                                            CTTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGA                                  <  SRR4302533.3433631/94‑1 (MQ=60)
                                                             TTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAG                                 >  SRR4302533.2357207/1‑94 (MQ=60)
                                                             TTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAG                                 <  SRR4302533.561242/94‑1 (MQ=60)
                                                             TTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAG                                 <  SRR4302533.561241/94‑1 (MQ=60)
                                                             TTTACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAC                                 >  SRR4302533.3408906/1‑94 (MQ=60)
                                                               TACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCC                               >  SRR4302533.5572988/1‑94 (MQ=60)
                                                               TACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCC                               >  SRR4302533.2195607/1‑94 (MQ=60)
                                                                ACTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCT                              <  SRR4302533.2835608/94‑1 (MQ=60)
                                                                 CTTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTT                             >  SRR4302533.3231148/1‑94 (MQ=60)
                                                                  TTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTC                            <  SRR4302533.233067/94‑1 (MQ=60)
                                                                  TTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTC                            <  SRR4302533.929939/94‑1 (MQ=60)
                                                                  TTTCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTC                            <  SRR4302533.3616228/94‑1 (MQ=60)
                                                                    TCGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGC                          >  SRR4302533.5584768/1‑94 (MQ=60)
                                                                     CGTTGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCC                         >  SRR4302533.4343525/1‑94 (MQ=60)
                                                                        TGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTG                      >  SRR4302533.5240290/1‑94 (MQ=60)
                                                                        TGCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTG                      <  SRR4302533.1049310/94‑1 (MQ=60)
                                                                         GCTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGT                     >  SRR4302533.6314228/1‑94 (MQ=60)
                                                                          CTACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTT                    >  SRR4302533.390995/1‑94 (MQ=60)
                                                                            ACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCG                  >  SRR4302533.990398/1‑94 (MQ=60)
                                                                            ACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCG                  <  SRR4302533.4440661/94‑1 (MQ=60)
                                                                            ACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCG                  <  SRR4302533.4315178/94‑1 (MQ=60)
                                                                            ACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCG                  >  SRR4302533.1444333/1‑94 (MQ=60)
                                                                            ACCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCG                  >  SRR4302533.1938899/1‑94 (MQ=60)
                                                                             CCGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGT                 >  SRR4302533.2534259/1‑94 (MQ=60)
                                                                              CGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGCGCCTTCGCCCTGTTCGTT                >  SRR4302533.3539286/1‑94 (MQ=60)
                                                                              CGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTT                >  SRR4302533.4381105/1‑94 (MQ=60)
                                                                              CGAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTT                >  SRR4302533.1419115/1‑94 (MQ=60)
                                                                               GAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTG               <  SRR4302533.448717/94‑1 (MQ=60)
                                                                               GAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTG               <  SRR4302533.1651675/94‑1 (MQ=60)
                                                                               GAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTG               >  SRR4302533.6195805/1‑94 (MQ=60)
                                                                               GAAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTG               >  SRR4302533.2868708/1‑94 (MQ=60)
                                                                                AAGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGA              >  SRR4302533.1064626/1‑94 (MQ=60)
                                                                                 AGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAG             <  SRR4302533.2640763/94‑1 (MQ=60)
                                                                                 AGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAG             <  SRR4302533.937334/94‑1 (MQ=60)
                                                                                 AGTCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAG             >  SRR4302533.1394003/1‑94 (MQ=60)
                                                                                   TCATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTAAGCG           <  SRR4302533.3373059/94‑1 (MQ=60)
                                                                                     ATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCGAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTT         <  SRR4302533.3202144/94‑1 (MQ=60)
                                                                                     ATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGCGCGTT         >  SRR4302533.2792258/1‑94 (MQ=60)
                                                                                     ATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTT         >  SRR4302533.2362779/1‑94 (MQ=60)
                                                                                     ATCTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTT         >  SRR4302533.299533/1‑94 (MQ=60)
                                                                                       CTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAC       >  SRR4302533.3938517/1‑94 (MQ=60)
                                                                                       CTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAC       >  SRR4302533.2393295/1‑94 (MQ=60)
                                                                                       CTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAC       >  SRR4302533.1148445/1‑94 (MQ=60)
                                                                                       CTGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAC       >  SRR4302533.6251951/1‑94 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      >  SRR4302533.3001061/1‑94 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      <  SRR4302533.1246050/94‑1 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      <  SRR4302533.3643413/94‑1 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      <  SRR4302533.1743556/94‑1 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      <  SRR4302533.5883087/94‑1 (MQ=60)
                                                                                        TGCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACC      <  SRR4302533.2982000/94‑1 (MQ=60)
                                                                                         GCCGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCG     <  SRR4302533.2789255/94‑1 (MQ=60)
                                                                                           CGCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGC   <  SRR4302533.2140752/94‑1 (MQ=60)
                                                                                            GCCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCA  >  SRR4302533.132203/1‑94 (MQ=60)
                                                                                             |                                                                                            
CGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCA  >  NC_000913/2647205‑2647390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑