Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,669,800 T→C C186R (TGT→CGT)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,8000TC100.0% 882.6 / NA 275C186R (TGT→CGT) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (120/155);  total (120/155)

Only 100 of 286 total aligned reads displayed.

TACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCC  >  NC_000913/2669710‑2669889
                                                                                          |                                                                                         
 acacCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGcgtg                                                                                        <  1:320102/93‑1 (MQ=255)
      gCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATg                                                                                   >  2:3080310/1‑93 (MQ=255)
      gCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATg                                                                                   <  2:748748/93‑1 (MQ=255)
      gCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATg                                                                                   >  2:4603666/1‑93 (MQ=255)
       ccAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATgg                                                                                  >  1:1174595/1‑93 (MQ=255)
         aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                <  2:4270046/93‑1 (MQ=255)
         aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                <  2:4324017/93‑1 (MQ=255)
         aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                >  1:5657783/1‑93 (MQ=255)
         aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                <  2:5572861/93‑1 (MQ=255)
           tAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAg                                                                              <  2:5421993/93‑1 (MQ=255)
              aCATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGa                                                                           <  1:972195/93‑1 (MQ=255)
               cATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGAc                                                                          >  1:3715802/1‑93 (MQ=255)
                 tGCCGCAACCGGACTTATTCGCCAACTGGCGTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCt                                                                        <  1:3339692/93‑1 (MQ=255)
                  gCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTg                                                                       <  1:792592/93‑1 (MQ=255)
                  gCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTg                                                                       >  1:5871994/1‑93 (MQ=255)
                  gCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTg                                                                       <  2:1697529/93‑1 (MQ=255)
                   ccGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTgc                                                                      >  2:81361/1‑93 (MQ=255)
                       aaCCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGAGTCGCCCCGCGTGGTATGGTCAGCGACCTGCGCgg                                                                  >  2:3176031/1‑93 (MQ=255)
                        aCCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGc                                                                 >  2:5021807/1‑93 (MQ=255)
                          cGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGccc                                                               <  1:6186490/93‑1 (MQ=255)
                            gACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCcac                                                             <  2:6076861/93‑1 (MQ=255)
                            gACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGACcac                                                             >  2:2091303/1‑93 (MQ=255)
                             aCTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCcaca                                                            >  2:5195524/1‑93 (MQ=255)
                              cTTATTCGCCAACTGGCTTATGACCTGGCACCGAAAGTGCGGGTGAATGGCGTCGCCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAg                                                           <  1:5852386/93‑1 (MQ=255)
                              cTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAg                                                           >  2:4896835/1‑93 (MQ=255)
                              cTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAg                                                           <  1:1590621/93‑1 (MQ=255)
                              cTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAg                                                           >  2:4534472/1‑93 (MQ=255)
                               ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          >  2:5873934/1‑93 (MQ=255)
                               ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          >  2:5374748/1‑93 (MQ=255)
                                tATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgc                                                         >  1:5269188/1‑93 (MQ=255)
                                tATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgc                                                         >  1:193189/1‑93 (MQ=255)
                                 aTTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgcg                                                        >  1:3657371/1‑93 (MQ=255)
                                  ttCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgcgc                                                       <  1:427696/93‑1 (MQ=255)
                                   tCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCt                                                      <  2:5098245/93‑1 (MQ=255)
                                    cGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTc                                                     <  2:5621822/93‑1 (MQ=255)
                                    cGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTc                                                     <  1:5552047/93‑1 (MQ=255)
                                    cGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTc                                                     <  2:4819528/93‑1 (MQ=255)
                                     gCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCg                                                    >  1:3633760/1‑93 (MQ=255)
                                      ccAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCgg                                                   <  1:1402979/93‑1 (MQ=255)
                                       cAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCggg                                                  >  2:2726707/1‑93 (MQ=255)
                                       cAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCggg                                                  >  2:147745/1‑93 (MQ=255)
                                       cAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCggg                                                  >  2:1042611/1‑93 (MQ=255)
                                        aaCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGc                                                 <  1:578716/93‑1 (MQ=255)
                                        aaCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGc                                                 >  1:2584900/1‑93 (MQ=255)
                                         aCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCa                                                >  1:644094/1‑93 (MQ=255)
                                           tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaaa                                              >  2:2577514/1‑93 (MQ=255)
                                           tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaaa                                              >  1:4357174/1‑93 (MQ=255)
                                           tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaaa                                              <  2:4255757/93‑1 (MQ=255)
                                           tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaaa                                              >  2:129620/1‑93 (MQ=255)
                                           tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGCATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaac                                              >  1:2251417/1‑92 (MQ=255)
                                            ggCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAg                                             >  1:548147/1‑93 (MQ=255)
                                             gCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGt                                            <  2:1203919/93‑1 (MQ=255)
                                              cTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTg                                           <  2:6360/93‑1 (MQ=255)
                                               ttATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          <  2:3153023/93‑1 (MQ=255)
                                               ttATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          <  1:4956856/93‑1 (MQ=255)
                                               tcaTGAACTTGCACCGAAAGTGCGGGTGACTGGCGCCGCCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          <  2:2298443/91‑1 (MQ=255)
                                                    aaCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCt                                     >  2:5481410/1‑93 (MQ=255)
                                                    aaCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCt                                     <  1:1260565/93‑1 (MQ=255)
                                                     aCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTc                                    >  1:3788078/1‑93 (MQ=255)
                                                     aCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTc                                    <  2:1083175/93‑1 (MQ=255)
                                                      cTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCg                                   >  1:5339046/1‑93 (MQ=255)
                                                      cTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCg                                   >  1:2666987/1‑93 (MQ=255)
                                                      cTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCg                                   >  1:6038628/1‑93 (MQ=255)
                                                       tGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGa                                  <  2:1789968/93‑1 (MQ=255)
                                                        ggCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGAt                                 <  2:2032984/93‑1 (MQ=255)
                                                          cACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaa                               >  1:2896560/1‑93 (MQ=255)
                                                          cACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaa                               <  2:2469384/93‑1 (MQ=255)
                                                           aCCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGTTATGTCCAGCGACCTGCGCTGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAt                              <  2:6141934/93‑1 (MQ=255)
                                                           aCCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAt                              >  1:5206226/1‑93 (MQ=255)
                                                            ccGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATg                             >  1:4453318/1‑93 (MQ=255)
                                                             cGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTTGATAATGc                            <  1:2767397/93‑1 (MQ=255)
                                                              gAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCa                           >  1:10467/1‑93 (MQ=255)
                                                               aaaGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAg                          <  2:4177834/93‑1 (MQ=255)
                                                                 aGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtc                        <  2:2788110/93‑1 (MQ=255)
                                                                 aGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtc                        <  1:3434715/93‑1 (MQ=255)
                                                                  gTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtct                       >  1:1309238/1‑93 (MQ=255)
                                                                  gTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtct                       >  2:895576/1‑93 (MQ=255)
                                                                   ggCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtctc                      <  2:3946093/92‑1 (MQ=255)
                                                                     cGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTg                    <  1:3854386/93‑1 (MQ=255)
                                                                       ggTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGAc                  >  2:2255267/1‑93 (MQ=255)
                                                                         tGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGc                <  1:4165889/93‑1 (MQ=255)
                                                                          gAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGcc               <  2:865254/93‑1 (MQ=255)
                                                                          gAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGcc               <  1:1807612/93‑1 (MQ=255)
                                                                           aaTGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCg              >  1:247282/1‑93 (MQ=255)
                                                                           aaTGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCg              <  2:3573679/93‑1 (MQ=255)
                                                                            aTGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCgg             <  2:3225063/93‑1 (MQ=255)
                                                                             tGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGga            <  1:3985973/93‑1 (MQ=255)
                                                                              gtcGTCCGCCCGCGTGGTATGGCCAGCGACGTGCGCGGCCCACAGGCTCTCGGGAAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgat           <  2:4090272/91‑2 (MQ=255)
                                                                              ggCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgag           >  1:4707251/1‑93 (MQ=255)
                                                                               gCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgaga          >  1:2069030/1‑93 (MQ=255)
                                                                               gCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgaga          >  1:3500354/1‑93 (MQ=255)
                                                                               gCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgaga          <  2:621477/93‑1 (MQ=255)
                                                                                cGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGaa         <  1:4415537/93‑1 (MQ=255)
                                                                                  tCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGaaaa       >  1:4576579/1‑93 (MQ=255)
                                                                                   cGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCTCTCGGGCAAAGTGAAACCACGATAATGCAGTCTCTGACGCCGGAGAAAAt      <  2:5794182/93‑1 (MQ=255)
                                                                                   cGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAt      <  1:4858814/93‑1 (MQ=255)
                                                                                     gCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTg    <  2:5275492/93‑1 (MQ=255)
                                                                                      cccGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTgc   <  2:3650607/93‑1 (MQ=255)
                                                                                       ccGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTgcc  >  2:6109352/1‑93 (MQ=255)
                                                                                       ccGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTgcc  <  2:271439/93‑1 (MQ=255)
                                                                                          |                                                                                         
TACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCC  >  NC_000913/2669710‑2669889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCGCCA  >  NC_000913/2669707‑2669893
                                                                                             |                                                                                             
CTGTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGC                                                                                               >  SRR4302533.4706838/1‑94 (MQ=60)
 TGTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCG                                                                                              <  SRR4302533.5313582/94‑1 (MQ=60)
 TGTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCG                                                                                              >  SRR4302533.3411223/1‑94 (MQ=60)
  GTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGT                                                                                             <  SRR4302533.4518612/94‑1 (MQ=60)
  GTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGT                                                                                             <  SRR4302533.3446376/94‑1 (MQ=60)
  GTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGT                                                                                             <  SRR4302533.368228/94‑1 (MQ=60)
   TACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTG                                                                                            <  SRR4302533.2040292/94‑1 (MQ=60)
    ACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGG                                                                                           <  SRR4302533.6085339/94‑1 (MQ=60)
    ACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGG                                                                                           <  SRR4302533.322062/94‑1 (MQ=60)
      CCCGCGAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTA                                                                                         <  SRR4302533.193538/94‑1 (MQ=60)
      CCCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTA                                                                                         <  SRR4302533.4746370/94‑1 (MQ=60)
      ACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTA                                                                                         <  SRR4302533.3642319/94‑1 (MQ=60)
       CCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTAT                                                                                        <  SRR4302533.5858869/94‑1 (MQ=60)
         GCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGG                                                                                      <  SRR4302533.2388286/94‑1 (MQ=60)
         GCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGG                                                                                      <  SRR4302533.718427/94‑1 (MQ=60)
         GCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGG                                                                                      >  SRR4302533.1181645/1‑94 (MQ=60)
           CAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCC                                                                                    <  SRR4302533.5639253/94‑1 (MQ=60)
           CAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCC                                                                                    >  SRR4302533.5691971/1‑94 (MQ=60)
           CAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCC                                                                                    <  SRR4302533.1741244/94‑1 (MQ=60)
             GTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAG                                                                                  >  SRR4302533.5123005/1‑94 (MQ=60)
               AAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCATCGGCCCGCGTGGTATGGCCAGCG                                                                                >  SRR4302533.4627721/1‑94 (MQ=60)
                 ACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGAC                                                                              >  SRR4302533.3738613/1‑94 (MQ=60)
                 ACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGAC                                                                              <  SRR4302533.3866662/94‑1 (MQ=60)
                 ACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGAC                                                                              <  SRR4302533.977904/94‑1 (MQ=60)
                  CATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACC                                                                             >  SRR4302533.3499966/1‑94 (MQ=60)
                    TGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTG                                                                           >  SRR4302533.5907488/1‑94 (MQ=60)
                    TGCCGCAACCGGACTTATTCGCCAACTGGCGTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTG                                                                           <  SRR4302533.3360333/94‑1 (MQ=60)
                     GCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGC                                                                          <  SRR4302533.797256/94‑1 (MQ=60)
                      CCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCG                                                                         <  SRR4302533.3792631/94‑1 (MQ=60)
                      CCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCG                                                                         <  SRR4302533.2857827/94‑1 (MQ=60)
                       CGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGC                                                                        <  SRR4302533.5004842/94‑1 (MQ=60)
                       CGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGC                                                                        <  SRR4302533.4376618/94‑1 (MQ=60)
                       CGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGC                                                                        >  SRR4302533.4428529/1‑94 (MQ=60)
                        GCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCG                                                                       <  SRR4302533.370930/94‑1 (MQ=60)
                        GCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCG                                                                       <  SRR4302533.4512269/94‑1 (MQ=60)
                         CAACCGGACTTATTCGCCCACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGGCCTGCGCGG                                                                      >  SRR4302533.4304825/1‑94 (MQ=60)
                         CAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGG                                                                      >  SRR4302533.387191/1‑94 (MQ=60)
                           ACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCC                                                                    >  SRR4302533.5464635/1‑94 (MQ=60)
                            CCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCC                                                                   >  SRR4302533.1058573/1‑94 (MQ=60)
                            CCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGGGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCC                                                                   >  SRR4302533.4960419/1‑94 (MQ=60)
                             CGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCA                                                                  >  SRR4302533.3534796/1‑94 (MQ=60)
                             CGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCA                                                                  >  SRR4302533.4653973/1‑94 (MQ=60)
                             CGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCA                                                                  <  SRR4302533.6223626/94‑1 (MQ=60)
                              GGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCAC                                                                 >  SRR4302533.4406392/1‑94 (MQ=60)
                              GGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCAC                                                                 >  SRR4302533.3711377/1‑94 (MQ=60)
                                 CTTATTCGCCAACTGGCTTATGACCTGGCACCGAAAGTGCGGGTGAATGGCGTCGCCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGG                                                              <  SRR4302533.5887768/94‑1 (MQ=60)
                                 CTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGG                                                              >  SRR4302533.5903015/1‑94 (MQ=60)
                                 CTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGG                                                              <  SRR4302533.1600241/94‑1 (MQ=60)
                                  TTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGC                                                             <  SRR4302533.4155258/94‑1 (MQ=60)
                                  TTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGC                                                             >  SRR4302533.5300794/1‑94 (MQ=60)
                                  TTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGC                                                             >  SRR4302533.194407/1‑94 (MQ=60)
                                   TATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCG                                                            >  SRR4302533.3679856/1‑94 (MQ=60)
                                   TATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCG                                                            >  SRR4302533.3679855/1‑94 (MQ=60)
                                    ATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGC                                                           >  SRR4302533.4472132/1‑94 (MQ=60)
                                    ATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGC                                                           >  SRR4302533.786041/1‑94 (MQ=60)
                                     TTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCT                                                          <  SRR4302533.430248/94‑1 (MQ=60)
                                       CGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCG                                                        >  SRR4302533.2661222/1‑94 (MQ=60)
                                       CGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCG                                                        <  SRR4302533.5585576/94‑1 (MQ=60)
                                       CGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCG                                                        >  SRR4302533.3656110/1‑94 (MQ=60)
                                         CCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGG                                                      <  SRR4302533.1411440/94‑1 (MQ=60)
                                         CCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGG                                                      <  SRR4302533.1581450/94‑1 (MQ=60)
                                         CCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGG                                                      >  SRR4302533.5425224/1‑94 (MQ=60)
                                          CAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGC                                                     >  SRR4302533.2569697/1‑94 (MQ=60)
                                          CAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGC                                                     >  SRR4302533.2600793/1‑94 (MQ=60)
                                           AACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCA                                                    >  SRR4302533.647886/1‑94 (MQ=60)
                                           AACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCA                                                    <  SRR4302533.582127/94‑1 (MQ=60)
                                           AACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCA                                                    >  SRR4302533.647885/1‑94 (MQ=60)
                                            ACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAA                                                   <  SRR4302533.2706436/94‑1 (MQ=60)
                                             CTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAA                                                  >  SRR4302533.4763209/1‑94 (MQ=60)
                                             CTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAA                                                  >  SRR4302533.4383474/1‑94 (MQ=60)
                                             CTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGCATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAC                                                  >  SRR4302533.2265336/1‑94 (MQ=60)
                                              TGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAG                                                 >  SRR4302533.551400/1‑94 (MQ=60)
                                                  TTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAA                                             <  SRR4302533.1200619/94‑1 (MQ=60)
                                                  TTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAA                                             <  SRR4302533.4986882/94‑1 (MQ=60)
                                                    ATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAAC                                           <  SRR4302533.5790472/94‑1 (MQ=60)
                                                     TGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACC                                          >  SRR4302533.1664377/1‑94 (MQ=60)
                                                     TGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACC                                          <  SRR4302533.2122862/94‑1 (MQ=60)
                                                     TGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACC                                          <  SRR4302533.2775089/94‑1 (MQ=60)
                                                      GAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCT                                         <  SRR4302533.6109210/94‑1 (MQ=60)
                                                      GAACTGGCACCGAAAGTGCGGGGGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCT                                         >  SRR4302533.5321036/1‑94 (MQ=60)
                                                       AACTGGCACCGAAAGTGCTGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTC                                        <  SRR4302533.6284625/94‑1 (MQ=60)
                                                       AACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTC                                        >  SRR4302533.5255589/1‑94 (MQ=60)
                                                       AACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTC                                        >  SRR4302533.3811275/1‑94 (MQ=60)
                                                       AACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTC                                        <  SRR4302533.1268105/94‑1 (MQ=60)
                                                        ACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCG                                       >  SRR4302533.5371060/1‑94 (MQ=60)
                                                        ACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCG                                       >  SRR4302533.6075223/1‑94 (MQ=60)
                                                        ACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCG                                       >  SRR4302533.2683351/1‑94 (MQ=60)
                                                        ACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCG                                       <  SRR4302533.5781541/94‑1 (MQ=60)
                                                         CTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGA                                      <  SRR4302533.3381203/94‑1 (MQ=60)
                                                         CTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGA                                      <  SRR4302533.844912/94‑1 (MQ=60)
                                                         CTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGA                                      >  SRR4302533.4985477/1‑94 (MQ=60)
                                                            GCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAA                                   >  SRR4302533.2914370/1‑94 (MQ=60)
                                                            GCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAA                                   >  SRR4302533.1268566/1‑94 (MQ=60)
                                                             CACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAT                                  <  SRR4302533.4596200/94‑1 (MQ=60)
                                                             CACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAT                                  >  SRR4302533.5237594/1‑94 (MQ=60)
                                                             CACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAT                                  >  SRR4302533.589959/1‑94 (MQ=60)
                                                              ACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATG                                 >  SRR4302533.4480243/1‑94 (MQ=60)
                                                              ACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGACCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATG                                 >  SRR4302533.2668766/1‑94 (MQ=60)
                                                                CGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTTGATAATGCA                               <  SRR4302533.2784372/94‑1 (MQ=60)
                                                                CGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCA                               >  SRR4302533.10553/1‑94 (MQ=60)
                                                                CGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCA                               <  SRR4302533.349137/94‑1 (MQ=60)
                                                                 GAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAG                              <  SRR4302533.3305873/94‑1 (MQ=60)
                                                                   AAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTC                            <  SRR4302533.2870355/94‑1 (MQ=60)
                                                                   AAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTC                            <  SRR4302533.4214476/94‑1 (MQ=60)
                                                                    AGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAATCCTCGATAATGCAGTCT                           <  SRR4302533.1188267/94‑1 (MQ=60)
                                                                    AGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCT                           >  SRR4302533.1317097/1‑94 (MQ=60)
                                                                    AGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCT                           <  SRR4302533.5837683/94‑1 (MQ=60)
                                                                    AGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCT                           <  SRR4302533.3455933/94‑1 (MQ=60)
                                                                     GTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTC                          >  SRR4302533.1902724/1‑94 (MQ=60)
                                                                      TGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCT                         <  SRR4302533.6042739/94‑1 (MQ=60)
                                                                       GCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTG                        <  SRR4302533.140440/94‑1 (MQ=60)
                                                                        CGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGA                       >  SRR4302533.3453630/1‑94 (MQ=60)
                                                                        CGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGA                       <  SRR4302533.3877927/94‑1 (MQ=60)
                                                                        CGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGA                       >  SRR4302533.2322732/1‑94 (MQ=60)
                                                                            TGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCC                   <  SRR4302533.4191132/94‑1 (MQ=60)
                                                                            TGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCC                   <  SRR4302533.3674538/94‑1 (MQ=60)
                                                                             GAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCG                  <  SRR4302533.1818611/94‑1 (MQ=60)
                                                                             GAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCG                  >  SRR4302533.258031/1‑94 (MQ=60)
                                                                             GAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCG                  >  SRR4302533.248810/1‑94 (MQ=60)
                                                                             GAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCG                  >  SRR4302533.393866/1‑94 (MQ=60)
                                                                              AATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGG                 <  SRR4302533.3315321/94‑1 (MQ=60)
                                                                              AATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGG                 <  SRR4302533.3942352/94‑1 (MQ=60)
                                                                              AATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGG                 <  SRR4302533.4152240/94‑1 (MQ=60)
                                                                               ATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGA                >  SRR4302533.5612621/1‑94 (MQ=60)
                                                                               ATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGA                <  SRR4302533.799566/94‑1 (MQ=60)
                                                                               ATGGCGTCGGCCCGCGGGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGA                >  SRR4302533.1302010/1‑94 (MQ=60)
                                                                                TGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAG               >  SRR4302533.4735784/1‑94 (MQ=60)
                                                                                TGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAG               <  SRR4302533.4010249/94‑1 (MQ=60)
                                                                                TGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAG               <  SRR4302533.5258392/94‑1 (MQ=60)
                                                                                 GGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGA              >  SRR4302533.3521940/1‑94 (MQ=60)
                                                                                 GGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGA              >  SRR4302533.2081773/1‑94 (MQ=60)
                                                                                   CGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAA            <  SRR4302533.3194899/94‑1 (MQ=60)
                                                                                   CGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAA            >  SRR4302533.1378580/1‑94 (MQ=60)
                                                                                   CGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAA            <  SRR4302533.4442202/94‑1 (MQ=60)
                                                                                    GTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAA           <  SRR4302533.5581424/94‑1 (MQ=60)
                                                                                    GTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAA           >  SRR4302533.4604316/1‑94 (MQ=60)
                                                                                     TCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAT          <  SRR4302533.5530234/94‑1 (MQ=60)
                                                                                      CGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATT         <  SRR4302533.4888265/94‑1 (MQ=60)
                                                                                         CCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCC      >  SRR4302533.4284536/1‑94 (MQ=60)
                                                                                          CCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCG     <  SRR4302533.2233306/94‑1 (MQ=60)
                                                                                          CCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCG     <  SRR4302533.159440/94‑1 (MQ=60)
                                                                                            GCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCGCC   >  SRR4302533.1049982/1‑94 (MQ=60)
                                                                                             CGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCGCCA  >  SRR4302533.5211530/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGTACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCCGCCA  >  NC_000913/2669707‑2669893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑