Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,460	T→C	I163T (ATC→ACC)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,460	0	T	C	100.0%	788.0 / NA	245	I163T (ATC→ACC)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (123/122); total (123/122)

Only 100 of 266 total aligned reads displayed.

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAccc                                                                                         >  1:1383755/1‑93 (MQ=255)

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACCCCAcccc                                                                                        >  2:205444/1‑92 (MQ=255)

   aaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCg                                                                                      >  1:2193046/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:1506548/1‑93 (MQ=255)

     ggTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGAc                                                                                    >  1:3416405/1‑93 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCCCCCTCGACt                                                                                   <  2:4657485/93‑1 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTg                                                                                  <  2:5115464/93‑1 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAAAACCACCCTCGACTg                                                                                  >  2:674931/1‑93 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTCCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGATGCCGTCTCCGACACCACCCTCCACTg                                                                                  >  2:52569/1‑93 (MQ=255)

        cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTgg                                                                                 >  1:271171/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                >  1:3924635/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                >  1:2749239/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACAACACCCTCGACTggg                                                                                >  1:3237155/1‑93 (MQ=255)

            aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgcgc                                                                             >  2:3394576/1‑93 (MQ=255)

            aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgcgc                                                                             <  1:6171373/93‑1 (MQ=255)

              gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCCACACCACCCTCGACTGGGCGCaa                                                                           >  2:5064761/1‑93 (MQ=255)

              gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCaa                                                                           <  1:4941867/93‑1 (MQ=255)

              gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCaa                                                                           >  2:377112/1‑93 (MQ=255)

              gCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCaa                                                                           <  1:4231873/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:2569013/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  1:5509668/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  1:5918823/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:1960395/93‑1 (MQ=255)

                 aGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAg                                                                        <  1:4764501/93‑1 (MQ=255)

                 aGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAg                                                                        <  2:580702/93‑1 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGc                                                                       <  2:4007084/93‑1 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGc                                                                       >  2:1389634/1‑93 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGc                                                                       >  1:6240061/1‑93 (MQ=255)

                    ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCga                                                                     >  2:2808404/1‑92 (MQ=255)

                    ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGt                                                                     <  2:1207371/93‑1 (MQ=255)

                    ttttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGt                                                                     >  1:3275522/1‑93 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  2:2159611/1‑93 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  1:4336328/1‑93 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGANTGGGCGCAACAGCGTAAGANgg                                                              >  1:5055783/1‑93 (MQ=255)

                           cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATgg                                                              <  1:5836105/93‑1 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGTCTGCCGTCTCCAACACCACCCTCGACTGGGAGCAACAGCGTAAGATggg                                                             >  2:3599668/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             >  1:1135188/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             >  2:2580965/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATggg                                                             >  1:3709344/1‑93 (MQ=255)

                            tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCCGCTGCCGTCTCCAACACCACCCTCGCCTGAGCGCAACAGCGTAAGAttcg                                                             >  1:2462945/1‑90 (MQ=255)

                             gtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGc                                                            <  1:5985503/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           <  1:4618126/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           >  2:5997487/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       <  1:4984962/93‑1 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       >  1:2892830/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       >  1:3809504/1‑93 (MQ=255)

                                   aTTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTCGGCGCAACAGCGTAAGATGGGCTTTTcc                                                      >  1:1018238/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCt                                                     >  2:428020/1‑93 (MQ=255)

                                       aaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                  >  1:266187/1‑93 (MQ=255)

                                       aaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACt                                                  <  2:5509558/93‑1 (MQ=255)

                                         cgcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAAATGGGCTTTTCCTACttt                                                <  1:630599/93‑1 (MQ=255)

                                          gcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTa                                               >  1:4922661/1‑93 (MQ=255)

                                          cccgCAAGCTGGCTTTTATTTCGCAATCGGCTGCCGTCTCCAACACCCCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTa                                               <  2:4387747/90‑1 (MQ=255)

                                           cgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  2:788739/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             >  1:2349687/1‑93 (MQ=255)

                                             gCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTg                                            <  2:1517944/93‑1 (MQ=255)

                                             gCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTg                                            >  2:2427433/1‑93 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           <  2:4848427/93‑1 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           <  1:5802526/93‑1 (MQ=255)

                                               aaGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                          <  2:5143994/93‑1 (MQ=255)

                                               aaGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                          >  2:5803643/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCACCAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         >  1:5205035/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         >  1:172852/1‑93 (MQ=255)

                                                 gCTGTCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCt                                        <  1:5459735/93‑1 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                       <  1:5151359/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                      >  1:410987/1‑93 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    >  2:6236877/1‑93 (MQ=255)

                                                      cGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCg                                   >  1:3024783/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACGCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 <  2:1856282/93‑1 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTCAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  1:5697685/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:845173/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  1:5369136/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGCCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 <  1:4539578/93‑1 (MQ=255)

                                                          tATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAg                               >  2:1537670/1‑93 (MQ=255)

                                                          tATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAg                               <  1:4474083/93‑1 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                              <  2:3943525/93‑1 (MQ=255)

                                                            tttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGcc                             <  2:4489303/93‑1 (MQ=255)

                                                              tCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTg                           <  1:881184/93‑1 (MQ=255)

                                                                gCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGa                         >  1:1630998/1‑93 (MQ=255)

                                                                 cAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGat                        >  1:4907466/1‑93 (MQ=255)

                                                                  aaTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGTCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGata                       <  2:105955/93‑1 (MQ=255)

                                                                  aaTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGata                       <  2:493817/93‑1 (MQ=255)

                                                                    tCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATc                     <  2:5875082/93‑1 (MQ=255)

                                                                     cGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCg                    <  2:932995/93‑1 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                  <  1:1959691/93‑1 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                  >  1:4554968/1‑93 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 >  1:1307920/1‑93 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  2:4753468/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  1:2544981/93‑1 (MQ=255)

                                                                          gCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt               >  2:4827760/1‑93 (MQ=255)

                                                                          gCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGtt               >  1:1172639/1‑93 (MQ=255)

                                                                           ccGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtg              >  2:109125/1‑93 (MQ=255)

                                                                            cGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtga             <  1:3087267/93‑1 (MQ=255)

                                                                                tcCATCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  1:3674254/93‑1 (MQ=255)

                                                                                tcCACCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:3188876/93‑1 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         >  2:3092500/1‑93 (MQ=255)

                                                                                 ccAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAt        >  2:5402449/1‑93 (MQ=255)

                                                                                  cAACACCCCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt       <  2:570746/93‑1 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    <  2:4193477/93‑1 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  <  2:3544215/93‑1 (MQ=255)

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  NC_000913/2718368‑2718553

CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACC                                                                                              >  SRR4302533.2707802/1‑94 (MQ=60)

CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACC                                                                                              <  SRR4302533.2094284/94‑1 (MQ=60)

CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACC                                                                                              >  SRR4302533.3464078/1‑94 (MQ=60)

 TGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCC                                                                                             >  SRR4302533.1392081/1‑94 (MQ=60)

  GGCAAGGTCTGAATTCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCT                                                                                            <  SRR4302533.2443534/94‑1 (MQ=60)

   GCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTC                                                                                           >  SRR4302533.1179343/1‑94 (MQ=60)

   GCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTC                                                                                           >  SRR4302533.5820644/1‑94 (MQ=60)

    CAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCG                                                                                          >  SRR4302533.2206559/1‑94 (MQ=60)

     AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302533.1515631/1‑94 (MQ=60)

      AGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGAC                                                                                        >  SRR4302533.3437512/1‑94 (MQ=60)

       GGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACT                                                                                       <  SRR4302533.3683309/94‑1 (MQ=60)

       GGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACT                                                                                       >  SRR4302533.2438106/1‑94 (MQ=60)

         TCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGG                                                                                     >  SRR4302533.272818/1‑94 (MQ=60)

          CTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGG                                                                                    >  SRR4302533.2766094/1‑94 (MQ=60)

          CTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGG                                                                                    >  SRR4302533.3948599/1‑94 (MQ=60)

          CTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGG                                                                                    <  SRR4302533.1094805/94‑1 (MQ=60)

          CTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACAACACCCTCGACTGGG                                                                                    >  SRR4302533.3257102/1‑94 (MQ=60)

           TGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGC                                                                                   <  SRR4302533.5861656/94‑1 (MQ=60)

              ATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCA                                                                                <  SRR4302533.6208459/94‑1 (MQ=60)

               TGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAA                                                                               <  SRR4302533.3829969/94‑1 (MQ=60)

                GCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAC                                                                              <  SRR4302533.4257449/94‑1 (MQ=60)

                GCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAC                                                                              <  SRR4302533.4971783/94‑1 (MQ=60)

                ACCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAC                                                                              <  SRR4302533.4992693/94‑1 (MQ=60)

                 CCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACA                                                                             <  SRR4302533.5954594/94‑1 (MQ=60)

                 CCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACA                                                                             <  SRR4302533.5542927/94‑1 (MQ=60)

                  CAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAG                                                                            >  SRR4302533.161159/1‑94 (MQ=60)

                   CGCTTTTCGCCTGTGCCGATTAAACGCGGAAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGC                                                                           <  SRR4302533.5973254/94‑1 (MQ=60)

                   AGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGC                                                                           >  SRR4302533.6277426/1‑94 (MQ=60)

                   AGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGC                                                                           <  SRR4302533.4793397/94‑1 (MQ=60)

                   AGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGC                                                                           >  SRR4302533.4365436/1‑94 (MQ=60)

                   AGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGC                                                                           >  SRR4302533.3275080/1‑94 (MQ=60)

                    GCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCG                                                                          <  SRR4302533.5499936/94‑1 (MQ=60)

                     CTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGT                                                                         >  SRR4302533.3295745/1‑94 (MQ=60)

                     CTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGT                                                                         <  SRR4302533.2491262/94‑1 (MQ=60)

                     CTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGT                                                                         <  SRR4302533.5920888/94‑1 (MQ=60)

                      TTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTA                                                                        >  SRR4302533.4362460/1‑94 (MQ=60)

                      TTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTA                                                                        <  SRR4302533.3727019/94‑1 (MQ=60)

                      TTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTA                                                                        >  SRR4302533.1498526/1‑94 (MQ=60)

                       TTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAA                                                                       >  SRR4302533.592247/1‑94 (MQ=60)

                       TTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAA                                                                       <  SRR4302533.4781798/94‑1 (MQ=60)

                        TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      <  SRR4302533.27295/94‑1 (MQ=60)

                        TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      <  SRR4302533.1152195/94‑1 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302533.5550551/94‑1 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    >  SRR4302533.4955871/1‑94 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302533.5089016/94‑1 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302533.5240615/94‑1 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    >  SRR4302533.96946/1‑94 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    >  SRR4302533.3023421/1‑94 (MQ=60)

                          CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    >  SRR4302533.1718722/1‑94 (MQ=60)

                            CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGANTGGGCGCAACAGCGTAAGANGG                                                                  >  SRR4302533.5086343/1‑94 (MQ=60)

                            CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGG                                                                  >  SRR4302533.772416/1‑94 (MQ=60)

                            CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGG                                                                  <  SRR4302533.4977856/94‑1 (MQ=60)

                             CTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGG                                                                 >  SRR4302533.3732126/1‑94 (MQ=60)

                             CTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGG                                                                 <  SRR4302533.5871370/94‑1 (MQ=60)

                             CTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGG                                                                 >  SRR4302533.1141939/1‑94 (MQ=60)

                             CTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCCGCTGCCGTCTCCAACACCACCCTCGCCTGAGCGCAACAGCGTAAGATtcg                                                                 >  SRR4302533.2478137/1‑91 (MQ=60)

                               GTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCT                                                               <  SRR4302533.6021751/94‑1 (MQ=60)

                                TGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTT                                                              <  SRR4302533.6296666/94‑1 (MQ=60)

                                TGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTT                                                              <  SRR4302533.1401391/94‑1 (MQ=60)

                                TGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTT                                                              <  SRR4302533.3159766/94‑1 (MQ=60)

                                TGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTT                                                              <  SRR4302533.4646113/94‑1 (MQ=60)

                                 gcgaATTAAACGCGGCAAGCTGGCCTTTATTTCGCAATAGGCTGCCGCCTCCAACACCCCCCTCGCCTGGGCGCAACAGCGTAAGATGGGCTTT                                                             <  SRR4302533.1270944/90‑1 (MQ=60)

                                   CGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTC                                                           >  SRR4302533.2910610/1‑94 (MQ=60)

                                   CGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTC                                                           >  SRR4302533.4413616/1‑94 (MQ=60)

                                   CGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTC                                                           >  SRR4302533.3832808/1‑94 (MQ=60)

                                    GATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCC                                                          <  SRR4302533.5015187/94‑1 (MQ=60)

                                    GATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTCGGCGCAACAGCGTAAGATGGGCTTTTCC                                                          >  SRR4302533.1024223/1‑94 (MQ=60)

                                     ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACATCTTAAGATGGGCTTTTTCT                                                         >  SRR4302533.4606964/1‑94 (MQ=60)

                                     ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         >  SRR4302533.2850262/1‑94 (MQ=60)

                                     ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         >  SRR4302533.4299719/1‑94 (MQ=60)

                                     ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         <  SRR4302533.5043263/94‑1 (MQ=60)

                                     ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         >  SRR4302533.3702824/1‑94 (MQ=60)

                                      TTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGGCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTA                                                        >  SRR4302533.522316/1‑94 (MQ=60)

                                       TAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAC                                                       <  SRR4302533.1661980/94‑1 (MQ=60)

                                        AAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACT                                                      >  SRR4302533.267806/1‑94 (MQ=60)

                                         AACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTT                                                     <  SRR4302533.5048289/94‑1 (MQ=60)

                                          ACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTT                                                    <  SRR4302533.3849724/94‑1 (MQ=60)

                                           CGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTA                                                   >  SRR4302533.4952483/1‑94 (MQ=60)

                                           CGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTA                                                   >  SRR4302533.4949784/1‑94 (MQ=60)

                                           CGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAAATGGGCTTTTCCTACTTTA                                                   <  SRR4302533.634307/94‑1 (MQ=60)

                                           CGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGGAAGAAGGGCTTTTCCTACTTTA                                                   >  SRR4302533.3127992/1‑94 (MQ=60)

                                            GCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAT                                                  >  SRR4302533.2888286/1‑94 (MQ=60)

                                             CGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATT                                                 >  SRR4302533.2364222/1‑94 (MQ=60)

                                             CGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATT                                                 <  SRR4302533.4509918/94‑1 (MQ=60)

                                              GGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAGCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTG                                                >  SRR4302533.4578617/1‑94 (MQ=60)

                                              GGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTG                                                <  SRR4302533.1928445/94‑1 (MQ=60)

                                               GCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGC                                               <  SRR4302533.5406594/94‑1 (MQ=60)

                                               GCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGC                                               >  SRR4302533.4945150/1‑94 (MQ=60)

                                               GCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGC                                               <  SRR4302533.1221922/94‑1 (MQ=60)

                                                CAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCG                                              <  SRR4302533.4331383/94‑1 (MQ=60)

                                                CAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCG                                              >  SRR4302533.1387896/1‑94 (MQ=60)

                                                CAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCG                                              <  SRR4302533.5837576/94‑1 (MQ=60)

                                                 AAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCACCAGCGTAAGATGGGCTTTTCCTACTTTATTGCGC                                             >  SRR4302533.5236400/1‑94 (MQ=60)

                                                 AAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGC                                             >  SRR4302533.173942/1‑94 (MQ=60)

                                                  AGCTGGCGTTTATGTCGCAATCGGCTGCCGTCTCCAACCCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCT                                            <  SRR4302533.3623177/94‑1 (MQ=60)

                                                   GCTGTCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTC                                           <  SRR4302533.5492629/94‑1 (MQ=60)

                                                   GCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTC                                           <  SRR4302533.5588720/94‑1 (MQ=60)

                                                    CTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCG                                          >  SRR4302533.413452/1‑94 (MQ=60)

                                                    CTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCG                                          <  SRR4302533.2661967/94‑1 (MQ=60)

                                                    CTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCG                                          <  SRR4302533.5182476/94‑1 (MQ=60)

                                                       GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       >  SRR4302533.3043288/1‑94 (MQ=60)

                                                         GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTCAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302533.5732102/1‑94 (MQ=60)

                                                         GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302533.5401388/1‑94 (MQ=60)

                                                         GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     <  SRR4302533.5334390/94‑1 (MQ=60)

                                                         GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302533.2754473/1‑94 (MQ=60)

                                                          TTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACA                                    <  SRR4302533.3907106/94‑1 (MQ=60)

                                                          TTTATTTCGCAATCGGCTGCCGCCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACA                                    <  SRR4302533.4567063/94‑1 (MQ=60)

                                                           TTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAG                                   <  SRR4302533.3703489/94‑1 (MQ=60)

                                                           TTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAG                                   >  SRR4302533.6222341/1‑94 (MQ=60)

                                                            TATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGC                                  <  SRR4302533.4501141/94‑1 (MQ=60)

                                                             ATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCC                                 >  SRR4302533.6235558/1‑94 (MQ=60)

                                                             ATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCC                                 >  SRR4302533.4806641/1‑94 (MQ=60)

                                                              TTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCT                                >  SRR4302533.520597/1‑94 (MQ=60)

                                                               TTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTG                               <  SRR4302533.2911341/94‑1 (MQ=60)

                                                               TTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTG                               <  SRR4302533.4570938/94‑1 (MQ=60)

                                                                TCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGG                              <  SRR4302533.886358/94‑1 (MQ=60)

                                                                TCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGG                              <  SRR4302533.4798425/94‑1 (MQ=60)

                                                                 CGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGA                             <  SRR4302533.2670544/94‑1 (MQ=60)

                                                                 CGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGA                             >  SRR4302533.1640877/1‑94 (MQ=60)

                                                                  GCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGAT                            <  SRR4302533.4598230/94‑1 (MQ=60)

                                                                  GCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGAT                            >  SRR4302533.4937199/1‑94 (MQ=60)

                                                                  GCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGAT                            <  SRR4302533.2345041/94‑1 (MQ=60)

                                                                   CAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGCTGGGGTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATC                           >  SRR4302533.132656/1‑94 (MQ=60)

                                                                   CAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATA                           <  SRR4302533.3967477/94‑1 (MQ=60)

                                                                      TCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCG                        <  SRR4302533.307457/94‑1 (MQ=60)

                                                                      TCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCG                        <  SRR4302533.6177013/94‑1 (MQ=60)

                                                                      TCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATAGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCG                        <  SRR4302533.3406460/94‑1 (MQ=60)

                                                                        GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      >  SRR4302533.4582594/1‑94 (MQ=60)

                                                                        GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      <  SRR4302533.1939373/94‑1 (MQ=60)

                                                                        GACTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      <  SRR4302533.6108794/94‑1 (MQ=60)

                                                                         GCTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACG                     <  SRR4302533.2714782/94‑1 (MQ=60)

                                                                         GCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACG                     >  SRR4302533.1315774/1‑94 (MQ=60)

                                                                         GCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACG                     <  SRR4302533.1971649/94‑1 (MQ=60)

                                                                           TGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTT                   <  SRR4302533.2560641/94‑1 (MQ=60)

                                                                           TGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTT                   >  SRR4302533.4238253/1‑94 (MQ=60)

                                                                           TGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTT                   >  SRR4302533.1179678/1‑94 (MQ=60)

                                                                              CGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                <  SRR4302533.3106249/94‑1 (MQ=60)

                                                                              CGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                <  SRR4302533.4054694/94‑1 (MQ=60)

                                                                                TCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGA              <  SRR4302533.3292076/94‑1 (MQ=60)

                                                                                 CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAA             >  SRR4302533.5509178/1‑94 (MQ=60)

                                                                                  TCCATCACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAT            <  SRR4302533.3696837/94‑1 (MQ=60)

                                                                                  TCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAT            <  SRR4302533.689522/94‑1 (MQ=60)

                                                                                   CCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATT           >  SRR4302533.2347409/1‑94 (MQ=60)

                                                                                     AACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGC         >  SRR4302533.5846386/1‑94 (MQ=60)

                                                                                          CACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGAC    >  SRR4302533.552411/1‑94 (MQ=60)

                                                                                          CACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGAC    >  SRR4302533.4920532/1‑94 (MQ=60)

                                                                                            CCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  SRR4302533.1546662/1‑94 (MQ=60)

CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  NC_000913/2718368‑2718553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`