Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,460	T→C	I163T (ATC→ACC)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,460	0	T	C	99.6%	849.4 / NA	264	I163T (ATC→ACC)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (137/126); minor base G (1/0); total (138/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                        <  2:3899707/93‑1 (MQ=255)

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                        <  1:6081695/93‑1 (MQ=255)

  cAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTc                                                                                       >  2:253238/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:8174871/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  2:5889892/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:3437285/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:2850246/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  2:4726963/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:10015527/1‑93 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACt                                                                                   <  1:9926870/93‑1 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTg                                                                                  >  1:5726703/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                <  2:8089755/93‑1 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                               >  1:6338087/1‑93 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                               <  2:7620223/93‑1 (MQ=255)

           aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgcg                                                                              >  1:6836002/1‑93 (MQ=255)

             tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCa                                                                            >  1:8771705/1‑93 (MQ=255)

             tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCa                                                                            <  2:4281432/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:1408842/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  1:7563616/93‑1 (MQ=255)

                cAGCCTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                         <  1:228022/93‑1 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGc                                                                       <  1:7847128/93‑1 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  2:7056394/1‑93 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  2:7382896/1‑93 (MQ=255)

                      ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTaa                                                                   <  1:9406527/93‑1 (MQ=255)

                       tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAg                                                                  >  1:9099742/1‑93 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTTGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  1:6572908/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:6563977/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:518592/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:830864/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCTTCTCCACCGCCTCCCTCGACTGGGCGCAACAGCGTAAGATg                                                               <  2:4615441/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATg                                                               >  1:5992345/1‑93 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           <  1:3178738/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           >  2:5659907/1‑93 (MQ=255)

                                ccGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAGCCTCGACTGGGCGCAACAGCGTAAGATGGGCttt                                                         >  1:6214327/1‑93 (MQ=255)

                                 cGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCtttt                                                        >  2:1660210/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       >  2:3690671/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       <  1:4538661/93‑1 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGCGCTTTTCCt                                                     >  1:458189/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGGCTCCAACACCACCCTCGTCTGGGCTCAACAGCGTAAGATTTGCTTTTCCt                                                     >  1:7524679/1‑93 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                    >  2:9690314/1‑93 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                    >  2:1136327/1‑93 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGTCTTTTCCTat                                                   >  1:7673985/1‑92 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAc                                                   >  1:3019887/1‑93 (MQ=255)

                                         cgcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACttt                                                <  2:2319504/93‑1 (MQ=255)

                                           cgacAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  2:6479196/90‑1 (MQ=255)

                                           cgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  1:6418854/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  2:1925515/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  1:2501278/93‑1 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGGGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           >  1:8370758/1‑93 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           <  2:7589576/93‑1 (MQ=255)

                                               aaGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                          >  1:231778/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         >  2:1826551/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         <  2:627673/93‑1 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                       <  1:8244638/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                      >  1:6970277/1‑93 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    <  1:6707002/93‑1 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    <  1:2684527/93‑1 (MQ=255)

                                                       gTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGa                                  >  1:1134499/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGaa                                 >  1:5970191/1‑92 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:1675759/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:240297/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:8645936/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  1:7981021/1‑93 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                              >  2:2946905/1‑93 (MQ=255)

                                                            tttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGcc                             <  1:5317038/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGccg                            >  1:4837229/1‑92 (MQ=255)

                                                              tCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTg                           >  2:6006166/1‑93 (MQ=255)

                                                                gCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGa                         <  2:9046940/93‑1 (MQ=255)

                                                                 cAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGTGCTCGGCGACAGCCTGGat                        >  1:3888225/1‑93 (MQ=255)

                                                                  aaTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGata                       <  1:9787287/93‑1 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                      <  2:7902445/93‑1 (MQ=255)

                                                                     cGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCg                    <  1:6760282/93‑1 (MQ=255)

                                                                      ggCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGa                   >  1:7222208/1‑93 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                  >  1:9585543/1‑93 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGCATATCGAc                  >  1:4022225/1‑93 (MQ=255)

                                                                        cTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 <  1:1496074/93‑1 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 <  2:8028102/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAGTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  2:9896196/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                >  2:7499699/1‑93 (MQ=255)

                                                                            cGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtga             >  2:4198260/1‑93 (MQ=255)

                                                                             gTCTCCAACCCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            <  2:9551372/93‑1 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            >  1:9350518/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            >  2:4388641/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            <  2:6647421/93‑1 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  2:6227477/1‑93 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  1:2101323/1‑93 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:5370957/93‑1 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:4197314/93‑1 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:8519524/93‑1 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCTCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:7220912/93‑1 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGTTCGGCGAAACCCTGGCTATCGACGTtgacgat         >  1:1252701/1‑92 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         >  2:2060841/1‑93 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:4002782/93‑1 (MQ=255)

                                                                                 ccAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAt        <  2:681717/93‑1 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt       >  2:2191475/1‑93 (MQ=255)

                                                                                    acacCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGc     >  1:8008162/1‑93 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    <  2:350838/93‑1 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    >  2:6118735/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  >  1:7075856/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  >  2:1318817/1‑93 (MQ=255)

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  NC_000913/2718367‑2718553

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAC                                                                                               >  SRR4302540.9632204/1‑94 (MQ=60)

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAC                                                                                               >  SRR4302540.4280759/1‑94 (MQ=60)

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAC                                                                                               <  SRR4302540.3622341/94‑1 (MQ=60)

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAC                                                                                               >  SRR4302540.3582390/1‑94 (MQ=60)

 CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACC                                                                                              >  SRR4302540.8157337/1‑94 (MQ=60)

 CTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACC                                                                                              >  SRR4302540.688254/1‑94 (MQ=60)

  TGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCC                                                                                             >  SRR4302540.6901393/1‑94 (MQ=60)

   GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCT                                                                                            >  SRR4302540.6555235/1‑94 (MQ=60)

    GCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTC                                                                                           <  SRR4302540.6149352/94‑1 (MQ=60)

    GCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTC                                                                                           <  SRR4302540.6824935/94‑1 (MQ=60)

    GCAAGCTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCTTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTC                                                                                           <  SRR4302540.7679070/94‑1 (MQ=60)

     CAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCG                                                                                          <  SRR4302540.5040582/94‑1 (MQ=60)

     CAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTAG                                                                                          <  SRR4302540.4849940/94‑1 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.8269823/1‑94 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.8711603/1‑94 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.2881121/1‑94 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.3474650/1‑94 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.6999896/1‑94 (MQ=60)

      AAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGA                                                                                         >  SRR4302540.10132116/1‑94 (MQ=60)

        GGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACT                                                                                       <  SRR4302540.7612989/94‑1 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      >  SRR4302540.5789763/1‑94 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      >  SRR4302540.517112/1‑94 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      <  SRR4302540.10042761/94‑1 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      <  SRR4302540.9469889/94‑1 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      <  SRR4302540.1269717/94‑1 (MQ=60)

         GTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTG                                                                                      >  SRR4302540.7301398/1‑94 (MQ=60)

          TCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGG                                                                                     >  SRR4302540.8448589/1‑94 (MQ=60)

            TGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGC                                                                                   <  SRR4302540.6275831/94‑1 (MQ=60)

            TGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGC                                                                                   >  SRR4302540.6408671/1‑94 (MQ=60)

             GAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCG                                                                                  <  SRR4302540.5564651/94‑1 (MQ=60)

             GAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCG                                                                                  >  SRR4302540.6911718/1‑94 (MQ=60)

             GAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCG                                                                                  >  SRR4302540.6911720/1‑94 (MQ=60)

              AATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGC                                                                                 >  SRR4302540.806774/1‑94 (MQ=60)

              AATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGC                                                                                 >  SRR4302540.9739520/1‑94 (MQ=60)

              AATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGC                                                                                 >  SRR4302540.6482999/1‑94 (MQ=60)

               ATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCA                                                                                >  SRR4302540.8873343/1‑94 (MQ=60)

                 GCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGTGCAAC                                                                              >  SRR4302540.8324262/1‑94 (MQ=60)

                  CCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACA                                                                             <  SRR4302540.857939/94‑1 (MQ=60)

                  CCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACA                                                                             <  SRR4302540.7648448/94‑1 (MQ=60)

                   CAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGGGCAACAG                                                                            >  SRR4302540.790877/1‑94 (MQ=60)

                   CAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAG                                                                            >  SRR4302540.3033978/1‑94 (MQ=60)

                   CAGCCTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAG                                                                            <  SRR4302540.230226/94‑1 (MQ=60)

                     GCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCG                                                                          <  SRR4302540.9258624/94‑1 (MQ=60)

                     GCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCG                                                                          <  SRR4302540.7938821/94‑1 (MQ=60)

                     GCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCAACTGGGCGCAACAGCC                                                                          >  SRR4302540.3117542/1‑94 (MQ=60)

                       TTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTA                                                                        >  SRR4302540.9241739/1‑94 (MQ=60)

                        TTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAA                                                                       <  SRR4302540.4111475/94‑1 (MQ=60)

                         TTCGCCTGTGGCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      <  SRR4302540.8898037/94‑1 (MQ=60)

                         TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      <  SRR4302540.9518396/94‑1 (MQ=60)

                         TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      >  SRR4302540.9209082/1‑94 (MQ=60)

                         TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAG                                                                      <  SRR4302540.9772474/94‑1 (MQ=60)

                         TTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTACAACACCACCCTCGACTGAGCGCAAacacgacaa                                                                      >  SRR4302540.9209081/1‑85 (MQ=60)

                           CGCCTGTGCCGATTAAACGCGGCAAGCTTGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302540.6645724/94‑1 (MQ=60)

                           CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302540.6645725/94‑1 (MQ=60)

                           CGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGAT                                                                    <  SRR4302540.5402837/94‑1 (MQ=60)

                            GCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATG                                                                   >  SRR4302540.6058732/1‑94 (MQ=60)

                             CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGTCTGGGCGCAACAGCGTAAGATGG                                                                  <  SRR4302540.7307784/94‑1 (MQ=60)

                             CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGG                                                                  <  SRR4302540.9606898/94‑1 (MQ=60)

                             CCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGG                                                                  >  SRR4302540.2192831/1‑94 (MQ=60)

                                 TGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTT                                                              <  SRR4302540.3213279/94‑1 (MQ=60)

                                  GCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAGCCTCGACTGGGCGCAACAGCGTCAGATGGGCTTT                                                             >  SRR4302540.6283473/1‑94 (MQ=60)

                                  GCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAGCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTT                                                             >  SRR4302540.6283472/1‑94 (MQ=60)

                                  GCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTT                                                             <  SRR4302540.5954967/94‑1 (MQ=60)

                                  GCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTATCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTT                                                             >  SRR4302540.2721893/1‑94 (MQ=60)

                                   CCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTT                                                            >  SRR4302540.5767076/1‑94 (MQ=60)

                                   CCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTT                                                            >  SRR4302540.1529553/1‑94 (MQ=60)

                                     GATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCC                                                          <  SRR4302540.4587784/94‑1 (MQ=60)

                                     GATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCC                                                          <  SRR4302540.2516971/94‑1 (MQ=60)

                                      ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         >  SRR4302540.7608870/1‑94 (MQ=60)

                                      ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGCGCTTTTCCT                                                         >  SRR4302540.463076/1‑94 (MQ=60)

                                      ATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGGCTCCAACACCACCCTCGTCTGGGCTCAACAGCGTAAGATTTGCTTTTCCT                                                         >  SRR4302540.7608871/1‑94 (MQ=60)

                                      ATTAAACGCGGCAAGCGGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCT                                                         <  SRR4302540.2198789/94‑1 (MQ=60)

                                        TAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGTCTTTTCCTAT                                                       >  SRR4302540.7760644/1‑94 (MQ=60)

                                        TAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAC                                                       >  SRR4302540.7760643/1‑94 (MQ=60)

                                        TAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAC                                                       >  SRR4302540.3053079/1‑94 (MQ=60)

                                        TAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAC                                                       <  SRR4302540.6212067/94‑1 (MQ=60)

                                         AAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACT                                                      >  SRR4302540.1106559/1‑94 (MQ=60)

                                         AAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACT                                                      >  SRR4302540.5387425/1‑94 (MQ=60)

                                           ACGCGGCAAGCTGGCTTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTT                                                    <  SRR4302540.2703353/94‑1 (MQ=60)

                                            CGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTA                                                   >  SRR4302540.2340038/1‑94 (MQ=60)

                                             GCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGGAACAGCGTAAGATGGGCTTTTCCTACTTTAT                                                  >  SRR4302540.8018160/1‑94 (MQ=60)

                                             GCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAT                                                  <  SRR4302540.6111367/94‑1 (MQ=60)

                                             GCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAT                                                  <  SRR4302540.6111368/94‑1 (MQ=60)

                                              CGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATT                                                 <  SRR4302540.6490297/94‑1 (MQ=60)

                                               GGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTG                                                >  SRR4302540.9199437/1‑94 (MQ=60)

                                               GGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTG                                                >  SRR4302540.2942468/1‑94 (MQ=60)

                                               GGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTG                                                <  SRR4302540.2528266/94‑1 (MQ=60)

                                                GCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGGGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGC                                               >  SRR4302540.8467904/1‑94 (MQ=60)

                                                 CAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCG                                              >  SRR4302540.234015/1‑94 (MQ=60)

                                                  AAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACACAGTAAGATTGGCTTTTCCTACTTTATTGCGC                                             >  SRR4302540.839227/1‑94 (MQ=60)

                                                     CTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCG                                          >  SRR4302540.7047758/1‑94 (MQ=60)

                                                     CTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCG                                          <  SRR4302540.8340459/94‑1 (MQ=60)

                                                       GGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGC                                        >  SRR4302540.4009667/1‑94 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       >  SRR4302540.7141666/1‑94 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       <  SRR4302540.2713552/94‑1 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       >  SRR4302540.7611072/1‑94 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       <  SRR4302540.4618144/94‑1 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       >  SRR4302540.6723770/1‑94 (MQ=60)

                                                        GCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCG                                       <  SRR4302540.6781020/94‑1 (MQ=60)

                                                         CGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGA                                      >  SRR4302540.1146325/1‑94 (MQ=60)

                                                         CGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGA                                      >  SRR4302540.1146324/1‑94 (MQ=60)

                                                          TTTTATTTGGCAAGCGGCTGCCGGCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     <  SRR4302540.785023/94‑1 (MQ=60)

                                                          GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302540.7134504/1‑94 (MQ=60)

                                                          GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302540.8073978/1‑94 (MQ=60)

                                                          GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAA                                     >  SRR4302540.6036273/1‑94 (MQ=60)

                                                          GTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCCCGACTGGGCGCCACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAA                                     >  SRR4302540.6036274/1‑94 (MQ=60)

                                                          GTGTATTTCGCAATCGGCTGCCGTCTACCACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAC                                     >  SRR4302540.8073979/1‑94 (MQ=60)

                                                           TTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACA                                    <  SRR4302540.9470168/94‑1 (MQ=60)

                                                              ATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCC                                 <  SRR4302540.3458724/94‑1 (MQ=60)

                                                              ATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCC                                 >  SRR4302540.8297650/1‑94 (MQ=60)

                                                               TTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCT                                <  SRR4302540.5375254/94‑1 (MQ=60)

                                                               TTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCG                                >  SRR4302540.4889050/1‑94 (MQ=60)

                                                                TTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTG                               <  SRR4302540.6852804/94‑1 (MQ=60)

                                                                TCCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTG                               <  SRR4302540.8174948/94‑1 (MQ=60)

                                                                 TCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGG                              <  SRR4302540.234917/94‑1 (MQ=60)

                                                                 TCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGG                              <  SRR4302540.3102445/94‑1 (MQ=60)

                                                                 TCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGG                              <  SRR4302540.6335768/94‑1 (MQ=60)

                                                                  CGCAATCGGCTGCCCTCTCCAACCCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGA                             <  SRR4302540.8138260/94‑1 (MQ=60)

                                                                   GCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGTGCTCGGCGACAGCCTGGAT                            >  SRR4302540.3930449/1‑94 (MQ=60)

                                                                     AATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATAT                          <  SRR4302540.9901960/94‑1 (MQ=60)

                                                                     AATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATAT                          <  SRR4302540.6466275/94‑1 (MQ=60)

                                                                      ATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATC                         >  SRR4302540.3874762/1‑94 (MQ=60)

                                                                       TCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCG                        <  SRR4302540.4071860/94‑1 (MQ=60)

                                                                        CGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGA                       >  SRR4302540.7302712/1‑94 (MQ=60)

                                                                        CGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGA                       <  SRR4302540.6834996/94‑1 (MQ=60)

                                                                        CGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGA                       <  SRR4302540.6834995/94‑1 (MQ=60)

                                                                        CGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGA                       >  SRR4302540.8598921/1‑94 (MQ=60)

                                                                         GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      <  SRR4302540.2712471/94‑1 (MQ=60)

                                                                         GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      >  SRR4302540.9698707/1‑94 (MQ=60)

                                                                         GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAC                      <  SRR4302540.4549093/94‑1 (MQ=60)

                                                                         GGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGCATATCGAC                      >  SRR4302540.4065575/1‑94 (MQ=60)

                                                                           CTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGT                    <  SRR4302540.1512540/94‑1 (MQ=60)

                                                                               CGTCTCCAACACCCCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                <  SRR4302540.9536657/94‑1 (MQ=60)

                                                                               CGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                >  SRR4302540.9461886/1‑94 (MQ=60)

                                                                               CGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                <  SRR4302540.4036495/94‑1 (MQ=60)

                                                                               CGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGAT                >  SRR4302540.2722505/1‑94 (MQ=60)

                                                                                GTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATG               >  SRR4302540.4585880/1‑94 (MQ=60)

                                                                                GTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATG               >  SRR4302540.2123882/1‑94 (MQ=60)

                                                                                GTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATG               >  SRR4302540.5920480/1‑94 (MQ=60)

                                                                                  CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGTTCGGCGAAACCCTGGCTATCGACGTTGAcgat             >  SRR4302540.1265514/1‑90 (MQ=60)

                                                                                  CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAA             >  SRR4302540.1265515/1‑94 (MQ=60)

                                                                                  CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAA             <  SRR4302540.5429990/94‑1 (MQ=60)

                                                                                  CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAA             <  SRR4302540.4242979/94‑1 (MQ=60)

                                                                                  CTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAA             <  SRR4302540.8618535/94‑1 (MQ=60)

                                                                                   TCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAT            >  SRR4302540.1899933/1‑94 (MQ=60)

                                                                                    CCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATT           <  SRR4302540.991814/94‑1 (MQ=60)

                                                                                      AACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGC         >  SRR4302540.8101338/1‑94 (MQ=60)

                                                                                        CACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTT       <  SRR4302540.3917162/94‑1 (MQ=60)

                                                                                        CACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTT       <  SRR4302540.4858467/94‑1 (MQ=60)

                                                                                         ACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG      >  SRR4302540.1176271/1‑94 (MQ=60)

                                                                                         ACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG      >  SRR4302540.7154593/1‑94 (MQ=60)

                                                                                           CACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGAC    >  SRR4302540.5810765/1‑94 (MQ=60)

                                                                                             CCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  SRR4302540.7142274/1‑94 (MQ=60)

CCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTA  >  NC_000913/2718367‑2718553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`