Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,435	A→G	L821L (TTA→TTG)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,435	0	A	G	99.6%	801.5 / NA	251	L821L (TTA→TTG)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (131/119); minor base C (1/0); total (132/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

AGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAA  >  NC_000913/2720345‑2720524

agagATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGATAGGATATAAAAGGGTTggg                                                                                         >  2:1229637/1‑93 (MQ=255)

 gagaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGc                                                                                        <  1:670793/93‑1 (MQ=255)

 gagaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGc                                                                                        >  1:4351838/1‑93 (MQ=255)

   gaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCtt                                                                                      >  1:400347/1‑93 (MQ=255)

   gaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCtt                                                                                      <  2:444809/93‑1 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     <  1:4630662/93‑1 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     >  2:2404168/1‑93 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     >  2:2226117/1‑93 (MQ=255)

     tCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAg                                                                                    >  2:5086744/1‑93 (MQ=255)

      ccgcGGCGTCACGCTTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGCACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAgg                                                                                   <  2:3463478/90‑1 (MQ=255)

      ccTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAgg                                                                                   <  2:2174247/93‑1 (MQ=255)

       cTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGt                                                                                  <  2:1209874/93‑1 (MQ=255)

       cTCGGCGTCACGCGTCCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGt                                                                                  <  2:1455031/93‑1 (MQ=255)

        tCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTc                                                                                 >  2:4257970/1‑93 (MQ=255)

        tCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTc                                                                                 <  2:5857312/93‑1 (MQ=255)

         cGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCg                                                                                >  1:5308835/1‑93 (MQ=255)

          ggCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGa                                                                               >  2:2631058/1‑93 (MQ=255)

           gCGTCACGCGTGCGATTTCCGATCCTGATCACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              <  2:6190804/93‑1 (MQ=255)

           gCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              >  2:2755637/1‑93 (MQ=255)

           gCGTCACGCGTGCGATTTCAGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              >  2:6138756/1‑93 (MQ=255)

             gTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGc                                                                            <  1:6092338/93‑1 (MQ=255)

                aCGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTa                                                                         >  1:2227146/1‑93 (MQ=255)

                aCGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTa                                                                         <  1:1473826/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        >  1:414142/1‑93 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:1043314/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:3898530/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:3823557/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        >  2:194452/1‑93 (MQ=255)

                  gcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAAt                                                                       <  2:5985736/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  2:1138760/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  1:892055/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  1:2206093/93‑1 (MQ=255)

                    gTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATgg                                                                     <  2:3325654/93‑1 (MQ=255)

                     tGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGa                                                                    >  1:1969257/1‑93 (MQ=255)

                     tGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGa                                                                    >  1:556694/1‑93 (MQ=255)

                      gCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGaa                                                                   <  2:3595052/93‑1 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                >  1:993448/1‑93 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                >  1:1120898/1‑93 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                <  2:6255776/93‑1 (MQ=255)

                             ccGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGa                                                            <  1:6194251/93‑1 (MQ=255)

                             ccGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGATCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGa                                                            >  1:4564163/1‑93 (MQ=255)

                               gATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAtt                                                          >  1:3254774/1‑93 (MQ=255)

                               gATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAtt                                                          <  2:36767/93‑1 (MQ=255)

                                aTCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTa                                                         >  2:4727126/1‑93 (MQ=255)

                                aTCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTa                                                         <  1:4482635/93‑1 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  1:4295354/1‑93 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  2:4549200/1‑93 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCACTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  1:2548300/1‑93 (MQ=255)

                                    tGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCta                                                     >  1:4387189/1‑93 (MQ=255)

                                    tGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCta                                                     >  2:4389182/1‑93 (MQ=255)

                                     gATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCtat                                                    >  2:4123542/1‑93 (MQ=255)

                                      aTAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCtata                                                   <  1:51142/93‑1 (MQ=255)

                                         aCATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATAcgc                                                <  1:4708060/93‑1 (MQ=255)

                                           aTCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCga                                              >  1:3102479/1‑93 (MQ=255)

                                           aTCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCga                                              <  2:5801055/93‑1 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  2:5018204/1‑93 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  1:4599891/1‑93 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  1:2848181/1‑93 (MQ=255)

                                             cGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgaga                                            <  2:4693738/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           >  2:5711357/1‑93 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           >  2:2843795/1‑93 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           <  1:3094143/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           <  2:2791133/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATCGAAAAGTTGATTACCTATACGCGAGAt                                           <  2:1886701/93‑1 (MQ=255)

                                               aTGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATc                                          >  1:5987577/1‑93 (MQ=255)

                                               aTGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATc                                          >  1:1009512/1‑93 (MQ=255)

                                                 gCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAc                                        <  1:1562226/93‑1 (MQ=255)

                                                 gCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAc                                        <  1:185639/93‑1 (MQ=255)

                                                   cGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACgg                                      <  1:3299892/93‑1 (MQ=255)

                                                     aaTTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGAc                                    >  1:1653391/1‑93 (MQ=255)

                                                        ttGCTGTACTGGTTCGCTCGGATCTCCAAGCGGTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATGCAGGACTAc                                 >  2:5351426/1‑93 (MQ=255)

                                                         tGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACa                                <  1:5637484/93‑1 (MQ=255)

                                                           cTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAc                              >  2:3052765/1‑93 (MQ=255)

                                                           cTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAc                              <  1:2011203/93‑1 (MQ=255)

                                                            tGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACg                             >  1:1260077/1‑93 (MQ=255)

                                                            tGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACg                             >  2:2287685/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           >  1:1413578/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           >  2:5253292/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           <  1:349978/93‑1 (MQ=255)

                                                               aCTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCt                          <  1:967588/93‑1 (MQ=255)

                                                                cTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTg                         >  2:2548877/1‑93 (MQ=255)

                                                                cTGGTTCGCTCGGATCTCAAAGGGGTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTg                         >  1:2164936/1‑93 (MQ=255)

                                                                  tgTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGaa                       <  1:4256382/92‑1 (MQ=255)

                                                                  ggTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGaa                       >  2:3179685/1‑93 (MQ=255)

                                                                   gTTCGCTCGTATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAAt                      <  1:244522/93‑1 (MQ=255)

                                                                    ttCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATg                     >  1:3729706/1‑93 (MQ=255)

                                                                      cGCTCGGATCTCAAGGGGGTCGGCTTAGGTCGACGCTTAATGGAAAAGTTGAGTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGt                   >  2:5773994/1‑93 (MQ=255)

                                                                      cGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGt                   <  2:1095487/93‑1 (MQ=255)

                                                                       gCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTa                  <  1:1182545/93‑1 (MQ=255)

                                                                         tCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTAtt                >  2:2969854/1‑93 (MQ=255)

                                                                          cGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTa               >  1:5853997/1‑93 (MQ=255)

                                                                            gATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACg             >  1:2604807/1‑93 (MQ=255)

                                                                            gATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACg             <  2:565275/93‑1 (MQ=255)

                                                                              tctcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGAt           >  1:4604824/1‑93 (MQ=255)

                                                                              tctcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCGGAATGGTATTACGAt           >  1:4218780/1‑93 (MQ=255)

                                                                                tcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGc         >  1:2081626/1‑93 (MQ=255)

                                                                                   aaGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGCATTACGATGCCaa      >  1:4895061/1‑93 (MQ=255)

                                                                                     gggTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAAc    >  1:3746497/1‑93 (MQ=255)

                                                                                     gggTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAAc    <  2:90959/93‑1 (MQ=255)

                                                                                       gTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACaa  <  1:1623062/93‑1 (MQ=255)

AGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAA  >  NC_000913/2720345‑2720524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGT  >  NC_000913/2720343‑2720528

GAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGG                                                                                              <  SRR4302533.1512574/94‑1 (MQ=60)

GAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGG                                                                                              <  SRR4302533.885880/94‑1 (MQ=60)

  AGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGC                                                                                            >  SRR4302533.4378098/1‑94 (MQ=60)

   GAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCT                                                                                           <  SRR4302533.674755/94‑1 (MQ=60)

    AGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTT                                                                                          >  SRR4302533.402763/1‑94 (MQ=60)

      ATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAG                                                                                        <  SRR4302533.3182946/94‑1 (MQ=60)

      ATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAG                                                                                        <  SRR4302533.4658726/94‑1 (MQ=60)

      ATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAG                                                                                        <  SRR4302533.348196/94‑1 (MQ=60)

         CTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTC                                                                                     >  SRR4302533.2196264/1‑94 (MQ=60)

          TCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCG                                                                                    >  SRR4302533.5340650/1‑94 (MQ=60)

           CGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGA                                                                                   >  SRR4302533.2735710/1‑94 (MQ=60)

            GGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAC                                                                                  <  SRR4302533.4281172/94‑1 (MQ=60)

               GTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCT                                                                               <  SRR4302533.6129154/94‑1 (MQ=60)

                TCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTT                                                                              >  SRR4302533.5921755/1‑94 (MQ=60)

                TCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTT                                                                              >  SRR4302533.1623272/1‑94 (MQ=60)

                 CACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTA                                                                             >  SRR4302533.2240894/1‑94 (MQ=60)

                  ACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAA                                                                            >  SRR4302533.416628/1‑94 (MQ=60)

                  ACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAA                                                                            <  SRR4302533.4379624/94‑1 (MQ=60)

                  ACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAA                                                                            <  SRR4302533.1482732/94‑1 (MQ=60)

                     CGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGG                                                                         <  SRR4302533.217082/94‑1 (MQ=60)

                     CGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGG                                                                         <  SRR4302533.2219697/94‑1 (MQ=60)

                     CGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGG                                                                         >  SRR4302533.4218245/1‑94 (MQ=60)

                     CGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGG                                                                         <  SRR4302533.897296/94‑1 (MQ=60)

                      GTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGA                                                                        <  SRR4302533.3805961/94‑1 (MQ=60)

                      GTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGA                                                                        >  SRR4302533.559996/1‑94 (MQ=60)

                      GTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGA                                                                        >  SRR4302533.1981300/1‑94 (MQ=60)

                      GTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGA                                                                        >  SRR4302533.1981301/1‑94 (MQ=60)

                       TGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAA                                                                       >  SRR4302533.4833592/1‑94 (MQ=60)

                        GCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAA                                                                      <  SRR4302533.1787369/94‑1 (MQ=60)

                        GCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAA                                                                      <  SRR4302533.694470/94‑1 (MQ=60)

                         CGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAA                                                                     <  SRR4302533.6111570/94‑1 (MQ=60)

                          GATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAG                                                                    <  SRR4302533.3672888/94‑1 (MQ=60)

                          GATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAG                                                                    >  SRR4302533.999254/1‑94 (MQ=60)

                          GATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAG                                                                    >  SRR4302533.1127548/1‑94 (MQ=60)

                             TTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTG                                                                 <  SRR4302533.5332415/94‑1 (MQ=60)

                              TCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGATCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGA                                                                >  SRR4302533.4591837/1‑94 (MQ=60)

                               CCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAT                                                               >  SRR4302533.3284024/1‑94 (MQ=60)

                               CCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAT                                                               >  SRR4302533.4466144/1‑94 (MQ=60)

                               CCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAT                                                               <  SRR4302533.6231418/94‑1 (MQ=60)

                                CGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATT                                                              >  SRR4302533.3274845/1‑94 (MQ=60)

                                CGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATT                                                              <  SRR4302533.834416/94‑1 (MQ=60)

                                CGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATT                                                              <  SRR4302533.4628266/94‑1 (MQ=60)

                                 GATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTA                                                             >  SRR4302533.4607182/1‑94 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            >  SRR4302533.4321186/1‑94 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            <  SRR4302533.4939890/94‑1 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            <  SRR4302533.4077393/94‑1 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            <  SRR4302533.4509780/94‑1 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            >  SRR4302533.2013318/1‑94 (MQ=60)

                                  ATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCACTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAC                                                            >  SRR4302533.2563985/1‑94 (MQ=60)

                                    CCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCT                                                          <  SRR4302533.4370044/94‑1 (MQ=60)

                                     CTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTA                                                         >  SRR4302533.4413676/1‑94 (MQ=60)

                                     CTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTA                                                         >  SRR4302533.37583/1‑94 (MQ=60)

                                        ATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATAC                                                      <  SRR4302533.51490/94‑1 (MQ=60)

                                         TAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACG                                                     >  SRR4302533.3117009/1‑94 (MQ=60)

                                         TAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACG                                                     <  SRR4302533.2485084/94‑1 (MQ=60)

                                         TAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACG                                                     >  SRR4302533.3508813/1‑94 (MQ=60)

                                           ACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCG                                                   <  SRR4302533.4736595/94‑1 (MQ=60)

                                            CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGA                                                  >  SRR4302533.1636770/1‑94 (MQ=60)

                                            CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGA                                                  <  SRR4302533.4643042/94‑1 (MQ=60)

                                            CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGA                                                  >  SRR4302533.3121552/1‑94 (MQ=60)

                                            CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGA                                                  >  SRR4302533.1560465/1‑94 (MQ=60)

                                            CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGA                                                  >  SRR4302533.6179138/1‑94 (MQ=60)

                                             ATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAG                                                 >  SRR4302533.2865706/1‑94 (MQ=60)

                                             ATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAG                                                 >  SRR4302533.4627758/1‑94 (MQ=60)

                                             ATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAG                                                 >  SRR4302533.1292870/1‑94 (MQ=60)

                                                GATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATC                                              >  SRR4302533.6023836/1‑94 (MQ=60)

                                                GATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATC                                              >  SRR4302533.1015417/1‑94 (MQ=60)

                                                GATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATC                                              <  SRR4302533.3113166/94‑1 (MQ=60)

                                                GATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATC                                              <  SRR4302533.989261/94‑1 (MQ=60)

                                                  TGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAC                                            >  SRR4302533.6042878/1‑94 (MQ=60)

                                                  AGCCGAATTTGCTGTACTGGTTCGCTCGGATCGCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAC                                            <  SRR4302533.1289917/94‑1 (MQ=60)

                                                   GCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACG                                           <  SRR4302533.3675105/94‑1 (MQ=60)

                                                   GCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACG                                           <  SRR4302533.1571630/94‑1 (MQ=60)

                                                   GCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACG                                           <  SRR4302533.186794/94‑1 (MQ=60)

                                                     CGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGA                                         <  SRR4302533.3320262/94‑1 (MQ=60)

                                                     CGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGGTGATTACCTATACGCGAGATCACGGA                                         >  SRR4302533.310484/1‑94 (MQ=60)

                                                      GAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGAC                                        <  SRR4302533.1155297/94‑1 (MQ=60)

                                                      GAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGAC                                        <  SRR4302533.1030726/94‑1 (MQ=60)

                                                      GAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGAC                                        >  SRR4302533.1663405/1‑94 (MQ=60)

                                                       AATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACT                                       >  SRR4302533.6314827/1‑94 (MQ=60)

                                                        ATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTA                                      <  SRR4302533.5820687/94‑1 (MQ=60)

                                                          TTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACA                                    <  SRR4302533.4543580/94‑1 (MQ=60)

                                                          TTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACA                                    >  SRR4302533.3153572/1‑94 (MQ=60)

                                                           TGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAA                                   <  SRR4302533.5671572/94‑1 (MQ=60)

                                                           TGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAA                                   >  SRR4302533.2231973/1‑94 (MQ=60)

                                                            GCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAC                                  <  SRR4302533.5610458/94‑1 (MQ=60)

                                                            GCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAC                                  >  SRR4302533.1659819/1‑94 (MQ=60)

                                                            GCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAC                                  <  SRR4302533.574820/94‑1 (MQ=60)

                                                            GCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAC                                  <  SRR4302533.4427010/94‑1 (MQ=60)

                                                             CTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACG                                 <  SRR4302533.2023594/94‑1 (MQ=60)

                                                             CTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACG                                 <  SRR4302533.4656450/94‑1 (MQ=60)

                                                             CTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACG                                 >  SRR4302533.1267614/1‑94 (MQ=60)

                                                             CTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACG                                 <  SRR4302533.4311922/94‑1 (MQ=60)

                                                              TGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGT                                >  SRR4302533.4599965/1‑94 (MQ=60)

                                                               GTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTC                               >  SRR4302533.1422127/1‑94 (MQ=60)

                                                               GTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTC                               >  SRR4302533.5593034/1‑94 (MQ=60)

                                                                TACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCT                              <  SRR4302533.352111/94‑1 (MQ=60)

                                                                TACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCT                              <  SRR4302533.1235360/94‑1 (MQ=60)

                                                                 ACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTG                             <  SRR4302533.142943/94‑1 (MQ=60)

                                                                 ACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTG                             >  SRR4302533.518457/1‑94 (MQ=60)

                                                                 ACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTG                             <  SRR4302533.973264/94‑1 (MQ=60)

                                                                 ACTGGTTCGCTCGGATCTCAAAGGGGTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTG                             >  SRR4302533.2178253/1‑94 (MQ=60)

                                                                  CTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGA                            >  SRR4302533.1897990/1‑94 (MQ=60)

                                                                    TGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAAT                          <  SRR4302533.4282043/94‑1 (MQ=60)

                                                                    GGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAAT                          <  SRR4302533.210894/94‑1 (MQ=60)

                                                                    GGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAAT                          <  SRR4302533.2230848/94‑1 (MQ=60)

                                                                     GTTCGCTCGTATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATG                         <  SRR4302533.246036/94‑1 (MQ=60)

                                                                     GTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATG                         >  SRR4302533.3752586/1‑94 (MQ=60)

                                                                     GTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATG                         <  SRR4302533.1500607/94‑1 (MQ=60)

                                                                      TTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGTACTACAACGTCTGAATGG                        >  SRR4302533.4380202/1‑94 (MQ=60)

                                                                      TTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGG                        >  SRR4302533.923860/1‑94 (MQ=60)

                                                                       TCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGT                       <  SRR4302533.5431255/94‑1 (MQ=60)

                                                                        CGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTA                      <  SRR4302533.4660847/94‑1 (MQ=60)

                                                                         GCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTAT                     <  SRR4302533.1189647/94‑1 (MQ=60)

                                                                         GCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTAT                     >  SRR4302533.2284468/1‑94 (MQ=60)

                                                                         GCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTAT                     >  SRR4302533.401715/1‑94 (MQ=60)

                                                                          CTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATT                    >  SRR4302533.4240437/1‑94 (MQ=60)

                                                                          CTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATT                    <  SRR4302533.3000335/94‑1 (MQ=60)

                                                                           TCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTA                   >  SRR4302533.5889387/1‑94 (MQ=60)

                                                                            CGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTAC                  <  SRR4302533.2475424/94‑1 (MQ=60)

                                                                             GGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACG                 >  SRR4302533.115823/1‑94 (MQ=60)

                                                                             GGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACG                 >  SRR4302533.2620809/1‑94 (MQ=60)

                                                                              GATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGA                >  SRR4302533.4316429/1‑94 (MQ=60)

                                                                               ATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGAT               >  SRR4302533.4632722/1‑94 (MQ=60)

                                                                               ATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGAT               >  SRR4302533.2611672/1‑94 (MQ=60)

                                                                               ATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGAT               <  SRR4302533.6282571/94‑1 (MQ=60)

                                                                               ATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCGGAATGGTATTACGAT               >  SRR4302533.4244295/1‑94 (MQ=60)

                                                                                 CTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGC             >  SRR4302533.2094436/1‑94 (MQ=60)

                                                                                  TCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCC            <  SRR4302533.1808036/94‑1 (MQ=60)

                                                                                   CAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCA           >  SRR4302533.2065998/1‑94 (MQ=60)

                                                                                   CAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCA           <  SRR4302533.4843020/94‑1 (MQ=60)

                                                                                    AAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAA          >  SRR4302533.2606630/1‑94 (MQ=60)

                                                                                    AAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGCATTACGATGCCAA          >  SRR4302533.4924727/1‑94 (MQ=60)

                                                                                      AGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAAC        >  SRR4302533.3769467/1‑94 (MQ=60)

                                                                                       GGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACA       >  SRR4302533.986553/1‑94 (MQ=60)

                                                                                         GTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAAT     <  SRR4302533.1632893/94‑1 (MQ=60)

                                                                                         GTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAAT     >  SRR4302533.1121391/1‑94 (MQ=60)

                                                                                          TTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATC    <  SRR4302533.1825804/94‑1 (MQ=60)

                                                                                          TTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATC    <  SRR4302533.2160886/94‑1 (MQ=60)

                                                                                           TGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCG   >  SRR4302533.1097692/1‑94 (MQ=60)

                                                                                            GGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGT  >  SRR4302533.3778527/1‑94 (MQ=60)

GAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGT  >  NC_000913/2720343‑2720528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`