Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,834,144	T→C	L435L (TTA→TTG)	hypF ←	transcriptional regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,834,144	0	T	C	100.0%	772.7 / NA	241	L435L (TTA→TTG)	hypF	transcriptional regulatory protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (120/121); total (120/121)

Only 100 of 250 total aligned reads displayed.

TTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCA  >  NC_000913/2834054‑2834233

ttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCaa                                                                                         <  2:6213566/93‑1 (MQ=255)

   aCGCGCCCACTGTCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGc                                                                                      <  1:859715/93‑1 (MQ=255)

   aCGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGc                                                                                      <  2:5514259/93‑1 (MQ=255)

     gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCtt                                                                                    <  1:6008745/93‑1 (MQ=255)

     gcgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCtt                                                                                    <  2:190894/93‑1 (MQ=255)

      cgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTc                                                                                   <  2:6044804/93‑1 (MQ=255)

      cgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTc                                                                                   <  2:1330017/93‑1 (MQ=255)

       gcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTcg                                                                                  >  2:1363294/1‑93 (MQ=255)

         ccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTcgcg                                                                                >  1:5956814/1‑93 (MQ=255)

            ctggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCa                                                                             >  1:2128221/1‑93 (MQ=255)

                 tggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCa                                                                        >  1:881577/1‑93 (MQ=255)

                 tggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCa                                                                        <  2:6307853/93‑1 (MQ=255)

                  ggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCAt                                                                       >  2:5365574/1‑93 (MQ=255)

                   gagaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATc                                                                      <  1:5694639/93‑1 (MQ=255)

                    agaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCt                                                                     <  2:2205688/93‑1 (MQ=255)

                       cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGa                                                                  <  2:1567024/93‑1 (MQ=255)

                       cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGa                                                                  <  2:780584/93‑1 (MQ=255)

                       cATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGa                                                                  <  1:1978458/93‑1 (MQ=255)

                           gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGcc                                                              >  1:1889562/1‑93 (MQ=255)

                           gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGcc                                                              >  2:2559466/1‑93 (MQ=255)

                           gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGcc                                                              >  2:4840454/1‑93 (MQ=255)

                            cccGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCa                                                             >  2:3620295/1‑93 (MQ=255)

                             ccGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCAt                                                            >  2:241422/1‑93 (MQ=255)

                             ccGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCAt                                                            >  2:5164064/1‑93 (MQ=255)

                              cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATc                                                           >  2:4596418/1‑93 (MQ=255)

                              cGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATc                                                           >  2:1699816/1‑93 (MQ=255)

                                 aTGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTc                                                        <  1:153190/93‑1 (MQ=255)

                                  tGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCa                                                       >  2:3845178/1‑93 (MQ=255)

                                  tGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCa                                                       >  2:695119/1‑93 (MQ=255)

                                   gcgcGTCATGCACAACGTATTGCGTGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAc                                                      <  2:4136798/93‑1 (MQ=255)

                                   gcgcGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAc                                                      >  2:102240/1‑93 (MQ=255)

                                   gcgcGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAc                                                      >  1:295768/1‑93 (MQ=255)

                                   gcgcGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAc                                                      >  2:2739123/1‑93 (MQ=255)

                                    cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACt                                                     >  1:1410136/1‑93 (MQ=255)

                                    cgcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACt                                                     >  2:4385532/1‑93 (MQ=255)

                                     gcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACtt                                                    >  2:4680844/1‑93 (MQ=255)

                                       gTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTaa                                                  <  2:5443400/93‑1 (MQ=255)

                                        tCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTaaa                                                 >  2:2145777/1‑93 (MQ=255)

                                        tCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTaaa                                                 >  2:1937044/1‑93 (MQ=255)

                                        tCATGCACAACGTATTGCGGGGGAAAATCGTAGATGGTTTGCATCAGGCGCAACGCTTCGCGCCGCTGCATCTGGATGCCGTCGTCACTTaaa                                                 >  2:2302103/1‑93 (MQ=255)

                                         cATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAAt                                                >  2:3724516/1‑93 (MQ=255)

                                         cATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAAt                                                <  2:910126/93‑1 (MQ=255)

                                         cATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAAt                                                <  1:604602/93‑1 (MQ=255)

                                           tGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCg                                              >  2:6110673/1‑93 (MQ=255)

                                           tGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCg                                              >  1:720115/1‑93 (MQ=255)

                                           tGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCg                                              >  1:5127318/1‑93 (MQ=255)

                                            gCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGc                                             >  2:4025145/1‑93 (MQ=255)

                                             cacaACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGcc                                            <  2:3276341/93‑1 (MQ=255)

                                                 aCGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGa                                        <  2:2803546/93‑1 (MQ=255)

                                                  cGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGAt                                       <  1:3754164/93‑1 (MQ=255)

                                                   gTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATg                                      >  1:5245595/1‑93 (MQ=255)

                                                   gTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATg                                      >  2:2488344/1‑93 (MQ=255)

                                                   gTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATg                                      <  1:1173017/93‑1 (MQ=255)

                                                     aTTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCt                                    <  1:930970/93‑1 (MQ=255)

                                                      ttGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTg                                   >  1:3335978/1‑93 (MQ=255)

                                                      ttGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTg                                   >  1:2337396/1‑93 (MQ=255)

                                                         cGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACt                                >  1:414541/1‑93 (MQ=255)

                                                          ggggTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTc                               <  1:2833369/93‑1 (MQ=255)

                                                          ggggTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTc                               >  2:29519/1‑93 (MQ=255)

                                                           gggTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCa                              >  1:6234193/1‑93 (MQ=255)

                                                           gggTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCa                              >  2:5640406/1‑93 (MQ=255)

                                                                aaaTCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACAcc                         <  1:1664266/93‑1 (MQ=255)

                                                                 aaTCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCg                        <  2:4953910/93‑1 (MQ=255)

                                                                 aaTCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCg                        >  2:5991630/1‑93 (MQ=255)

                                                                   tCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCt                      >  1:3158030/1‑93 (MQ=255)

                                                                   tCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCt                      <  2:3147242/93‑1 (MQ=255)

                                                                     gTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTg                    >  1:2317252/1‑93 (MQ=255)

                                                                     gTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTg                    >  2:3647637/1‑93 (MQ=255)

                                                                     gTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTg                    <  2:386399/93‑1 (MQ=255)

                                                                      tAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGt                   <  2:2533463/93‑1 (MQ=255)

                                                                      tAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGt                   >  2:671952/1‑93 (MQ=255)

                                                                       aGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGtt                  >  1:769678/1‑93 (MQ=255)

                                                                       aGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGtt                  <  1:1627149/93‑1 (MQ=255)

                                                                        gATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTc                 >  1:4886428/1‑93 (MQ=255)

                                                                        gATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTc                 <  1:5781163/93‑1 (MQ=255)

                                                                         aTGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCa                <  1:259830/93‑1 (MQ=255)

                                                                          tGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAc               <  1:1371018/93‑1 (MQ=255)

                                                                          tGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAc               >  1:3057101/1‑93 (MQ=255)

                                                                           gTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAcc              >  2:3510025/1‑93 (MQ=255)

                                                                            ttttGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACcg             <  1:4770955/93‑1 (MQ=255)

                                                                            ttttGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAAAACCGCTTGTTCAAcg             >  1:5027550/1‑93 (MQ=255)

                                                                             tttGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACcgc            >  1:5876645/1‑93 (MQ=255)

                                                                             tttGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACcgc            <  1:4466172/93‑1 (MQ=255)

                                                                                gCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTCAATCGCCCAGATGCTGACTCAACCCCGCTTGTTCCCCGCGCa         <  1:3385561/93‑1 (MQ=255)

                                                                                  aTCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCAcc       <  2:2081444/93‑1 (MQ=255)

                                                                                  aTCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCAcc       <  2:627825/93‑1 (MQ=255)

                                                                                  aTCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCAcc       >  1:625099/1‑93 (MQ=255)

                                                                                  aTCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCAcc       >  1:2558349/1‑93 (MQ=255)

                                                                                  aTCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGCTCACCGCGCAcc       >  1:3876636/1‑93 (MQ=255)

                                                                                   tCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCa      <  1:3976250/93‑1 (MQ=255)

                                                                                   tCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCa      >  2:3244044/1‑93 (MQ=255)

                                                                                    cAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAg     <  1:1150018/93‑1 (MQ=255)

                                                                                    cAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAg     <  2:5889585/93‑1 (MQ=255)

                                                                                    cAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAg     <  1:4716693/93‑1 (MQ=255)

                                                                                      ggCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGc   >  1:18312/1‑93 (MQ=255)

                                                                                      ggCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGc   >  1:2086759/1‑93 (MQ=255)

                                                                                      ggCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGc   >  2:431258/1‑93 (MQ=255)

                                                                                      ggCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGACCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGc   <  1:4442572/93‑1 (MQ=255)

                                                                                       gcgcAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCa  <  1:3574722/93‑1 (MQ=255)

                                                                                       gcgcAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCa  <  1:3688172/93‑1 (MQ=255)

TTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCA  >  NC_000913/2834054‑2834233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAG  >  NC_000913/2834057‑2834237

ACGCGCCCACTGTCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCT                                                                                         <  SRR4302533.864753/94‑1 (MQ=60)

 CGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCTCAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTT                                                                                        <  SRR4302533.3470280/94‑1 (MQ=60)

 CGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTT                                                                                        <  SRR4302533.5558741/94‑1 (MQ=60)

  GCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTC                                                                                       <  SRR4302533.5696045/94‑1 (MQ=60)

  GCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTC                                                                                       >  SRR4302533.4918709/1‑94 (MQ=60)

  GCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTC                                                                                       <  SRR4302533.6045149/94‑1 (MQ=60)

   CGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCCACGCTTCG                                                                                      >  SRR4302533.2421880/1‑94 (MQ=60)

   CGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCG                                                                                      <  SRR4302533.1789934/94‑1 (MQ=60)

    CCCCACTGGCTTGAGACATAGCCCGGATGCGCGTCATGCACAACGGATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGC                                                                                     <  SRR4302533.4805512/94‑1 (MQ=60)

     CCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCG                                                                                    >  SRR4302533.5992848/1‑94 (MQ=60)

        ACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCA                                                                                 >  SRR4302533.2141346/1‑94 (MQ=60)

          TGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACT                                                                               >  SRR4302533.1394426/1‑94 (MQ=60)

             CTGGAGACATAGCCCGGATGCGCGTCCTGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCCACGCTTCGCGCCACTGCA                                                                            >  SRR4302533.5860872/1‑94 (MQ=60)

             CTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCA                                                                            >  SRR4302533.886756/1‑94 (MQ=60)

              TGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCAT                                                                           >  SRR4302533.3021314/1‑94 (MQ=60)

                GAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCT                                                                         <  SRR4302533.4669902/94‑1 (MQ=60)

                GAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCT                                                                         <  SRR4302533.5729029/94‑1 (MQ=60)

                 AGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTG                                                                        <  SRR4302533.4333066/94‑1 (MQ=60)

                 AGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTG                                                                        <  SRR4302533.3174406/94‑1 (MQ=60)

                  GACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGG                                                                       <  SRR4302533.6269503/94‑1 (MQ=60)

                  GACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGG                                                                       <  SRR4302533.2007044/94‑1 (MQ=60)

                    CATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAGAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGAT                                                                     <  SRR4302533.806659/94‑1 (MQ=60)

                    CATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGAT                                                                     <  SRR4302533.1990575/94‑1 (MQ=60)

                    CATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGAT                                                                     <  SRR4302533.5872153/94‑1 (MQ=60)

                     ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATG                                                                    >  SRR4302533.5080164/1‑94 (MQ=60)

                     ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATG                                                                    >  SRR4302533.159745/1‑94 (MQ=60)

                       AGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCC                                                                  >  SRR4302533.1901102/1‑94 (MQ=60)

                       AGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCC                                                                  >  SRR4302533.321475/1‑94 (MQ=60)

                        GCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCA                                                                 <  SRR4302533.1870343/94‑1 (MQ=60)

                         CCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCAT                                                                <  SRR4302533.515660/94‑1 (MQ=60)

                         CCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCAT                                                                <  SRR4302533.6041055/94‑1 (MQ=60)

                         CCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCAT                                                                >  SRR4302533.1281445/1‑94 (MQ=60)

                          CCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATC                                                               <  SRR4302533.269101/94‑1 (MQ=60)

                          CCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATC                                                               <  SRR4302533.4513488/94‑1 (MQ=60)

                            GGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGT                                                             >  SRR4302533.395304/1‑94 (MQ=60)

                              ATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCA                                                           <  SRR4302533.154170/94‑1 (MQ=60)

                              ATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCA                                                           <  SRR4302533.1426866/94‑1 (MQ=60)

                               TGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAC                                                          >  SRR4302533.297574/1‑94 (MQ=60)

                                GCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACT                                                         <  SRR4302533.4088239/94‑1 (MQ=60)

                                GCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACT                                                         <  SRR4302533.5765/94‑1 (MQ=60)

                                GCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACT                                                         <  SRR4302533.13189/94‑1 (MQ=60)

                                GCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACT                                                         >  SRR4302533.1418663/1‑94 (MQ=60)

                                 CGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTT                                                        >  SRR4302533.1637775/1‑94 (MQ=60)

                                    GTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAA                                                     >  SRR4302533.3956264/1‑94 (MQ=60)

                                    GTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAA                                                     >  SRR4302533.2613476/1‑94 (MQ=60)

                                      CATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATC                                                   <  SRR4302533.5211715/94‑1 (MQ=60)

                                      CATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATC                                                   <  SRR4302533.608152/94‑1 (MQ=60)

                                       ATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCG                                                  >  SRR4302533.2884371/1‑94 (MQ=60)

                                       ATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCG                                                  >  SRR4302533.4205375/1‑94 (MQ=60)

                                       ATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCG                                                  >  SRR4302533.5158293/1‑94 (MQ=60)

                                       ATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCG                                                  >  SRR4302533.724363/1‑94 (MQ=60)

                                        TGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGC                                                 <  SRR4302533.1995966/94‑1 (MQ=60)

                                            CAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAG                                             <  SRR4302533.1845863/94‑1 (MQ=60)

                                              ACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGAT                                           >  SRR4302533.1518714/1‑94 (MQ=60)

                                               CGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATG                                          >  SRR4302533.5277140/1‑94 (MQ=60)

                                               CGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATG                                          <  SRR4302533.6314388/94‑1 (MQ=60)

                                               CGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATG                                          <  SRR4302533.3777175/94‑1 (MQ=60)

                                                GTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGC                                         <  SRR4302533.1180057/94‑1 (MQ=60)

                                                 TATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCT                                        <  SRR4302533.3029312/94‑1 (MQ=60)

                                                  ATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTG                                       >  SRR4302533.3356593/1‑94 (MQ=60)

                                                  ATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTG                                       <  SRR4302533.936434/94‑1 (MQ=60)

                                                  ATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTG                                       >  SRR4302533.2351863/1‑94 (MQ=60)

                                                  ATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTG                                       >  SRR4302533.2795298/1‑94 (MQ=60)

                                                  ATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTG                                       >  SRR4302533.319604/1‑94 (MQ=60)

                                                   TTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGA                                      >  SRR4302533.2341746/1‑94 (MQ=60)

                                                    TGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGAC                                     <  SRR4302533.2471813/94‑1 (MQ=60)

                                                     GCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACT                                    >  SRR4302533.417031/1‑94 (MQ=60)

                                                       GGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCA                                  <  SRR4302533.2850812/94‑1 (MQ=60)

                                                       GGGGTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCA                                  >  SRR4302533.6271518/1‑94 (MQ=60)

                                                         GGTAAAATCGTAGATGTTTTGCACCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAAC                                <  SRR4302533.2219134/94‑1 (MQ=60)

                                                          GTAAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACA                               >  SRR4302533.116794/1‑94 (MQ=60)

                                                            AAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGGTGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACC                             <  SRR4302533.482643/94‑1 (MQ=60)

                                                            AAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACC                             >  SRR4302533.1954936/1‑94 (MQ=60)

                                                            AAAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACC                             <  SRR4302533.2136094/94‑1 (MQ=60)

                                                             AAATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCG                            <  SRR4302533.1674325/94‑1 (MQ=60)

                                                               ATCGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCT                          >  SRR4302533.3177464/1‑94 (MQ=60)

                                                                 CGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTG                        <  SRR4302533.2289387/94‑1 (MQ=60)

                                                                 CGTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTG                        >  SRR4302533.2331598/1‑94 (MQ=60)

                                                                  GTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGT                       >  SRR4302533.4485041/1‑94 (MQ=60)

                                                                  GTAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGT                       >  SRR4302533.669152/1‑94 (MQ=60)

                                                                   TAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTT                      >  SRR4302533.774209/1‑94 (MQ=60)

                                                                   TAGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTT                      >  SRR4302533.6108711/1‑94 (MQ=60)

                                                                    AGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTC                     >  SRR4302533.4916034/1‑94 (MQ=60)

                                                                    AGATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTC                     <  SRR4302533.1637005/94‑1 (MQ=60)

                                                                     GATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCA                    <  SRR4302533.825067/94‑1 (MQ=60)

                                                                     GATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCA                    <  SRR4302533.5816079/94‑1 (MQ=60)

                                                                      ATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAC                   <  SRR4302533.261408/94‑1 (MQ=60)

                                                                      ATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAC                   <  SRR4302533.3735097/94‑1 (MQ=60)

                                                                      ATGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCAC                   >  SRR4302533.3075855/1‑94 (MQ=60)

                                                                       TGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACC                  >  SRR4302533.6281305/1‑94 (MQ=60)

                                                                       TGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACC                  <  SRR4302533.1379257/94‑1 (MQ=60)

                                                                       TGTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACC                  <  SRR4302533.915847/94‑1 (MQ=60)

                                                                        GTTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAAAACCGCTTGTTCAACG                 >  SRR4302533.5057994/1‑94 (MQ=60)

                                                                         TTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGC                >  SRR4302533.5912170/1‑94 (MQ=60)

                                                                         TTTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGC                <  SRR4302533.4799871/94‑1 (MQ=60)

                                                                          TTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCG               <  SRR4302533.4493184/94‑1 (MQ=60)

                                                                          TTTGCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCAATTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCG               <  SRR4302533.3400496/94‑1 (MQ=60)

                                                                             GCATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTCAATCGCCCAGATGCTGACTCAACCCCGCTTGTTCCCCGCGCAC            <  SRR4302533.3406465/94‑1 (MQ=60)

                                                                              CATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACC           >  SRR4302533.628770/1‑94 (MQ=60)

                                                                              CATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACC           >  SRR4302533.3507292/1‑94 (MQ=60)

                                                                              CATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACC           >  SRR4302533.6081968/1‑94 (MQ=60)

                                                                              CATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACC           >  SRR4302533.2574080/1‑94 (MQ=60)

                                                                              CATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGCTCACCGCGCACC           >  SRR4302533.3900297/1‑94 (MQ=60)

                                                                               ATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCA          <  SRR4302533.957426/94‑1 (MQ=60)

                                                                               ATCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCA          >  SRR4302533.3758055/1‑94 (MQ=60)

                                                                                TCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAG         >  SRR4302533.927879/1‑94 (MQ=60)

                                                                                TCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAG         <  SRR4302533.883791/94‑1 (MQ=60)

                                                                                TCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAG         <  SRR4302533.4000457/94‑1 (MQ=60)

                                                                                TCAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAG         <  SRR4302533.3886924/94‑1 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        <  SRR4302533.753172/94‑1 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        <  SRR4302533.1156886/94‑1 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        <  SRR4302533.1638735/94‑1 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        >  SRR4302533.3620439/1‑94 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        <  SRR4302533.4745289/94‑1 (MQ=60)

                                                                                 CAGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGG        <  SRR4302533.4752103/94‑1 (MQ=60)

                                                                                  AGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGC       >  SRR4302533.2099609/1‑94 (MQ=60)

                                                                                  AGGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGC       >  SRR4302533.18437/1‑94 (MQ=60)

                                                                                   GGCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGACCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCA      <  SRR4302533.4469434/94‑1 (MQ=60)

                                                                                    GCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAG     <  SRR4302533.3710838/94‑1 (MQ=60)

                                                                                    GCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAG     <  SRR4302533.6003221/94‑1 (MQ=60)

                                                                                    GCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAG     <  SRR4302533.3596756/94‑1 (MQ=60)

                                                                                    GCGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAG     <  SRR4302533.5956441/94‑1 (MQ=60)

                                                                                     CGCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGA    >  SRR4302533.5526232/1‑94 (MQ=60)

                                                                                      GCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAA   <  SRR4302533.459352/94‑1 (MQ=60)

                                                                                      GCAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAA   <  SRR4302533.2251047/94‑1 (MQ=60)

                                                                                       CAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAG  <  SRR4302533.241146/94‑1 (MQ=60)

ACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAG  >  NC_000913/2834057‑2834237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`