Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,316	T→C	T154A (ACT→GCT)	hycF ←	putative iron‑sulfur protein of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,316	0	T	C	99.6%	760.3 / NA	239	T154A (ACT→GCT)	hycF	putative iron‑sulfur protein of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (124/113); total (126/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGA  >  NC_000913/2842226‑2842405

  gCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCa                                                                                       <  2:5971665/93‑1 (MQ=255)

    tcatcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCaaa                                                                                     >  2:170458/1‑93 (MQ=255)

    tcatcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCaaa                                                                                     >  1:2423352/1‑93 (MQ=255)

    tcatcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCaaa                                                                                     <  1:5940085/93‑1 (MQ=255)

      atcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGc                                                                                   >  1:2858959/1‑93 (MQ=255)

       tcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCt                                                                                  <  2:2843623/93‑1 (MQ=255)

       tcaGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCt                                                                                  >  2:3609222/1‑93 (MQ=255)

        caGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTACTGGCGCTTACATTCCGGGCAAGCCTCAAAGCtt                                                                                 >  2:4186677/1‑93 (MQ=255)

         aGATGGCCTCTTTCATATGGCTAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCttt                                                                                <  2:551215/93‑1 (MQ=255)

         aGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCttt                                                                                <  2:3971203/93‑1 (MQ=255)

          gATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTc                                                                               <  1:1256275/93‑1 (MQ=255)

            tGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTcgc                                                                             <  2:2976472/93‑1 (MQ=255)

             ggCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTcgcg                                                                            >  1:6230957/1‑93 (MQ=255)

             ggCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTcgcg                                                                            <  1:5499475/93‑1 (MQ=255)

              gCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCgg                                                                           <  2:239/93‑1 (MQ=255)

               ccTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCggt                                                                          <  1:5565574/93‑1 (MQ=255)

                ctctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCggtg                                                                         <  2:4395272/93‑1 (MQ=255)

                 tctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCTAGCCTCAAAGCTTTCGcggcgg                                                                        >  2:1233279/1‑93 (MQ=255)

                 tctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCggtgg                                                                        >  1:3974048/1‑93 (MQ=255)

                 tctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCggtgg                                                                        >  1:4623434/1‑93 (MQ=255)

                   tttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGtt                                                                      >  1:413139/1‑93 (MQ=255)

                   tttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGtt                                                                      <  1:4858304/93‑1 (MQ=255)

                   tttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGtt                                                                      <  1:1313371/93‑1 (MQ=255)

                    ttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGttt                                                                     >  1:2954948/1‑93 (MQ=255)

                     tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGtttt                                                                    >  2:3772691/1‑93 (MQ=255)

                     tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGtttt                                                                    >  2:5014238/1‑93 (MQ=255)

                      cATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTc                                                                   >  1:376377/1‑93 (MQ=255)

                      cATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTc                                                                   <  2:5557514/93‑1 (MQ=255)

                       atatGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTcc                                                                  >  1:2028256/1‑93 (MQ=255)

                       atatGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTcc                                                                  <  2:5381063/93‑1 (MQ=255)

                        tatGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCcg                                                                 >  1:4976642/1‑93 (MQ=255)

                         atGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCcgc                                                                <  1:138957/93‑1 (MQ=255)

                           ggCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGCTTTCcgcgc                                                              >  1:2900247/1‑93 (MQ=255)

                                gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTg                                                         >  1:322570/1‑93 (MQ=255)

                                gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTg                                                         >  1:2229369/1‑93 (MQ=255)

                                gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTg                                                         >  2:4865126/1‑93 (MQ=255)

                                gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTg                                                         >  1:5241073/1‑93 (MQ=255)

                                gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTg                                                         >  1:5138281/1‑93 (MQ=255)

                                  cAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTc                                                       <  1:2573817/93‑1 (MQ=255)

                                  cAGTACAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTc                                                       <  2:628865/93‑1 (MQ=255)

                                   aGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCg                                                      >  2:4104409/1‑93 (MQ=255)

                                   aGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCg                                                      >  1:4048279/1‑93 (MQ=255)

                                    gTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGc                                                     >  1:2150004/1‑93 (MQ=255)

                                       cAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGt                                                  <  2:3707400/93‑1 (MQ=255)

                                       cAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGCTTTCCGCGCGGCTGTCGCCGt                                                  <  2:4972649/93‑1 (MQ=255)

                                        aaTACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGtt                                                 >  1:5671445/1‑93 (MQ=255)

                                          tcgggTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTtgt                                               <  2:2346332/90‑1 (MQ=255)

                                          tACGGTCGGACGGCACCAGGCATTTCTGGCGATTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTtgt                                               <  1:942245/93‑1 (MQ=255)

                                            cGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGc                                             >  2:4443757/1‑93 (MQ=255)

                                               tCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTa                                          <  1:1886644/93‑1 (MQ=255)

                                                cGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTaa                                         >  1:187390/1‑93 (MQ=255)

                                                cGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTaa                                         >  1:3675989/1‑93 (MQ=255)

                                                  gACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagc                                       >  2:5856765/1‑93 (MQ=255)

                                                  gACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagc                                       >  2:3825730/1‑93 (MQ=255)

                                                  gACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagc                                       >  2:2107699/1‑93 (MQ=255)

                                                   aCGCCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagca                                      <  2:3469422/93‑1 (MQ=255)

                                                    cGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagcag                                     <  1:1436454/93‑1 (MQ=255)

                                                    cGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagcag                                     <  2:1938017/93‑1 (MQ=255)

                                                     ggCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAagcagc                                    <  1:3688872/93‑1 (MQ=255)

                                                      gCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAgcg                                   <  1:3089490/93‑1 (MQ=255)

                                                        cccAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCa                                 <  2:494568/92‑1 (MQ=255)

                                                        aCCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCa                                 >  2:1556023/1‑93 (MQ=255)

                                                        aCCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCa                                 >  2:1434587/1‑93 (MQ=255)

                                                         ccAGGCATTTCTGGCGCTTACATCCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCaa                                <  1:4569832/93‑1 (MQ=255)

                                                           aGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATg                              <  2:427323/93‑1 (MQ=255)

                                                           aGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATg                              >  2:2671481/1‑93 (MQ=255)

                                                           aGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATg                              <  1:3537110/93‑1 (MQ=255)

                                                           aGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATg                              <  1:4303066/93‑1 (MQ=255)

                                                            ggCATTGCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATgg                             <  1:2663302/93‑1 (MQ=255)

                                                             gCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGc                            <  1:6130117/93‑1 (MQ=255)

                                                             gCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGc                            >  2:5670031/1‑93 (MQ=255)

                                                              cATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCg                           >  1:1143082/1‑93 (MQ=255)

                                                               aTTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGt                          <  1:3015731/93‑1 (MQ=255)

                                                                tttCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATCGCGTa                         >  2:2051058/1‑93 (MQ=255)

                                                                tttCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCAGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTa                         <  2:3974048/93‑1 (MQ=255)

                                                                tgtCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTa                         <  2:2195445/91‑1 (MQ=255)

                                                                 ttCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAg                        >  2:4346864/1‑93 (MQ=255)

                                                                  tCTGGCGCTTACATTCCGGGCAAGCCTCACAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGt                       <  2:6190801/93‑1 (MQ=255)

                                                                  tCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGt                       <  2:5487862/93‑1 (MQ=255)

                                                                   cTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTc                      <  2:6063511/93‑1 (MQ=255)

                                                                     ggCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGa                    >  1:3432418/1‑93 (MQ=255)

                                                                         cTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGAtctc                >  1:4784151/1‑93 (MQ=255)

                                                                           tACATTCCGGGCAAGCCTCAAAGTTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGGTGTGCTTAAGCAGCGCAATAGCGTAGTCGATATCtt              >  2:5108487/1‑93 (MQ=255)

                                                                            aCATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCttt             >  2:4617593/1‑93 (MQ=255)

                                                                            aCATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCttt             <  1:6272575/93‑1 (MQ=255)

                                                                             cATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCtttt            >  2:1629499/1‑92 (MQ=255)

                                                                             cATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTc            <  2:2755512/93‑1 (MQ=255)

                                                                               ttCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTg          >  1:605018/1‑93 (MQ=255)

                                                                               ttCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTg          >  1:5405701/1‑93 (MQ=255)

                                                                               ttCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTg          >  1:5721309/1‑93 (MQ=255)

                                                                               ttCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGATGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTg          >  1:2047975/1‑93 (MQ=255)

                                                                                  cGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGAc       >  2:2445202/1‑93 (MQ=255)

                                                                                   gggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACg      <  1:1745122/93‑1 (MQ=255)

                                                                                   gggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACg      >  1:1691818/1‑93 (MQ=255)

                                                                                    ggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACgg     >  2:3896933/1‑93 (MQ=255)

                                                                                    ggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACgg     <  1:424542/93‑1 (MQ=255)

                                                                                    ggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACgg     <  2:4719936/93‑1 (MQ=255)

                                                                                    ggCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACgg     >  2:456165/1‑93 (MQ=255)

                                                                                     gCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGc    <  1:1622146/93‑1 (MQ=255)

                                                                                      cAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCg   >  1:3747960/1‑93 (MQ=255)

TTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGA  >  NC_000913/2842226‑2842405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGG  >  NC_000913/2842223‑2842409

AAATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACAGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGC                                                                                               >  SRR4302533.2319268/1‑94 (MQ=60)

 AATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCC                                                                                              <  SRR4302533.6313429/94‑1 (MQ=60)

 AATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCC                                                                                              >  SRR4302533.1987315/1‑94 (MQ=60)

  ATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCT                                                                                             <  SRR4302533.74359/94‑1 (MQ=60)

  ATTGCTCATCAGAGGGCCTCTTTCATATGGCTAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCT                                                                                             <  SRR4302533.5024004/94‑1 (MQ=60)

      CTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAA                                                                                         >  SRR4302533.2438311/1‑94 (MQ=60)

      CTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAA                                                                                         >  SRR4302533.4283630/1‑94 (MQ=60)

       TCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAG                                                                                        >  SRR4302533.1994965/1‑94 (MQ=60)

       TCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAG                                                                                        <  SRR4302533.5976000/94‑1 (MQ=60)

        CATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGC                                                                                       >  SRR4302533.577720/1‑94 (MQ=60)

        CATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGC                                                                                       >  SRR4302533.2876548/1‑94 (MQ=60)

        CATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGC                                                                                       <  SRR4302533.288917/94‑1 (MQ=60)

         ATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCT                                                                                      >  SRR4302533.615499/1‑94 (MQ=60)

         ATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCT                                                                                      >  SRR4302533.2993541/1‑94 (MQ=60)

         ATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCT                                                                                      >  SRR4302533.4021125/1‑94 (MQ=60)

           CAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTT                                                                                    <  SRR4302533.1633047/94‑1 (MQ=60)

           CAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGCGCAAGCCTCAAAGCTTT                                                                                    >  SRR4302533.2971472/1‑94 (MQ=60)

            AGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTC                                                                                   <  SRR4302533.3703412/94‑1 (MQ=60)

            AGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTC                                                                                   >  SRR4302533.6287922/1‑94 (MQ=60)

             GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCG                                                                                  >  SRR4302533.2554817/1‑94 (MQ=60)

             GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCG                                                                                  <  SRR4302533.1263785/94‑1 (MQ=60)

               TGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCG                                                                                <  SRR4302533.6306170/94‑1 (MQ=60)

               TGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCG                                                                                >  SRR4302533.6268273/1‑94 (MQ=60)

                GGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGG                                                                               <  SRR4302533.1542868/94‑1 (MQ=60)

                GGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGG                                                                               <  SRR4302533.5532648/94‑1 (MQ=60)

                 GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGT                                                                              >  SRR4302533.614660/1‑94 (MQ=60)

                  CCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTG                                                                             <  SRR4302533.4629974/94‑1 (MQ=60)

                  CCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTG                                                                             <  SRR4302533.5599212/94‑1 (MQ=60)

                   CTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCACAGCTTTTGCGGTGG                                                                            >  SRR4302533.5156497/1‑94 (MQ=60)

                   CTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGG                                                                            >  SRR4302533.4651458/1‑94 (MQ=60)

                   CTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGG                                                                            >  SRR4302533.5583115/1‑94 (MQ=60)

                   CTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGG                                                                            >  SRR4302533.3998246/1‑94 (MQ=60)

                    TCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGT                                                                           >  SRR4302533.6216953/1‑94 (MQ=60)

                     CTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTT                                                                          >  SRR4302533.415620/1‑94 (MQ=60)

                     CTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTT                                                                          >  SRR4302533.1361563/1‑94 (MQ=60)

                      TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTT                                                                         <  SRR4302533.4887750/94‑1 (MQ=60)

                      TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTT                                                                         >  SRR4302533.2973017/1‑94 (MQ=60)

                      TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTT                                                                         <  SRR4302533.1321252/94‑1 (MQ=60)

                       TTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTT                                                                        <  SRR4302533.4893353/94‑1 (MQ=60)

                        TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTC                                                                       >  SRR4302533.378647/1‑94 (MQ=60)

                         CATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCC                                                                      >  SRR4302533.5162945/1‑94 (MQ=60)

                         CATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCC                                                                      >  SRR4302533.2141095/1‑94 (MQ=60)

                         CATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCC                                                                      <  SRR4302533.5405942/94‑1 (MQ=60)

                         CATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCC                                                                      >  SRR4302533.2040777/1‑94 (MQ=60)

                          ATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCG                                                                     >  SRR4302533.5006820/1‑94 (MQ=60)

                          ATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCG                                                                     >  SRR4302533.2814813/1‑94 (MQ=60)

                           TATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGC                                                                    >  SRR4302533.3539361/1‑94 (MQ=60)

                            ATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCG                                                                   <  SRR4302533.139841/94‑1 (MQ=60)

                             TGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGCTTTCCGCGC                                                                  >  SRR4302533.2918066/1‑94 (MQ=60)

                                CGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGC                                                               >  SRR4302533.3924082/1‑94 (MQ=60)

                                 GAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCT                                                              >  SRR4302533.414245/1‑94 (MQ=60)

                                  AGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTG                                                             >  SRR4302533.324540/1‑94 (MQ=60)

                                  AGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTG                                                             >  SRR4302533.2243129/1‑94 (MQ=60)

                                  AGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTG                                                             >  SRR4302533.5169330/1‑94 (MQ=60)

                                  AGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTG                                                             >  SRR4302533.5272579/1‑94 (MQ=60)

                                   GTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGT                                                            <  SRR4302533.2819990/94‑1 (MQ=60)

                                    TCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTT                                                           >  SRR4302533.3574284/1‑94 (MQ=60)

                                     CAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCG                                                          >  SRR4302533.5905270/1‑94 (MQ=60)

                                     CAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCG                                                          <  SRR4302533.2589650/94‑1 (MQ=60)

                                     CAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCG                                                          >  SRR4302533.4072932/1‑94 (MQ=60)

                                     CAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCG                                                          <  SRR4302533.2078797/94‑1 (MQ=60)

                                      AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGC                                                         >  SRR4302533.2163244/1‑94 (MQ=60)

                                      AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGC                                                         >  SRR4302533.3748959/1‑94 (MQ=60)

                                      AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGCCGC                                                         >  SRR4302533.3135476/1‑94 (MQ=60)

                                       GTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCC                                                        <  SRR4302533.2975884/94‑1 (MQ=60)

                                          CAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCCAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTT                                                     >  SRR4302533.541812/1‑94 (MQ=60)

                                          CAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTT                                                     >  SRR4302533.5705696/1‑94 (MQ=60)

                                          CAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTT                                                     <  SRR4302533.4864962/94‑1 (MQ=60)

                                             TACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTG                                                  >  SRR4302533.3748785/1‑94 (MQ=60)

                                             TACGGTCGGACGGCACCAGGCATTTCTGGCGATTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTG                                                  <  SRR4302533.947787/94‑1 (MQ=60)

                                                GGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTT                                               >  SRR4302533.5754029/1‑94 (MQ=60)

                                                GGTCGGAAGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGACTCAAAGCTTTGGCGGAGGATTTCCGCGCGGCTGTCGCCGTTGTGCTT                                               >  SRR4302533.956837/1‑94 (MQ=60)

                                                  TCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAA                                             >  SRR4302533.1441151/1‑94 (MQ=60)

                                                  TCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAA                                             >  SRR4302533.3698580/1‑94 (MQ=60)

                                                  TCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAA                                             >  SRR4302533.188555/1‑94 (MQ=60)

                                                  TCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAA                                             <  SRR4302533.1898167/94‑1 (MQ=60)

                                                     GACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCA                                          >  SRR4302533.4759738/1‑94 (MQ=60)

                                                      ACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAG                                         <  SRR4302533.1961876/94‑1 (MQ=60)

                                                       CGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGC                                        <  SRR4302533.1445128/94‑1 (MQ=60)

                                                       CGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGC                                        <  SRR4302533.2047981/94‑1 (MQ=60)

                                                        GGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCG                                       <  SRR4302533.3711539/94‑1 (MQ=60)

                                                        GGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCG                                       <  SRR4302533.1347687/94‑1 (MQ=60)

                                                         GCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGC                                      <  SRR4302533.3108480/94‑1 (MQ=60)

                                                           ACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAA                                    >  SRR4302533.4230699/1‑94 (MQ=60)

                                                           ACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAA                                    >  SRR4302533.1894004/1‑94 (MQ=60)

                                                            CCAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAT                                   >  SRR4302533.3460889/1‑94 (MQ=60)

                                                            CCAGGCATTTCTGGCGCTTACATCCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAT                                   <  SRR4302533.4597526/94‑1 (MQ=60)

                                                             CAGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATG                                  <  SRR4302533.4486076/94‑1 (MQ=60)

                                                              AGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGG                                 >  SRR4302533.2175054/1‑94 (MQ=60)

                                                              AGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGG                                 <  SRR4302533.4328956/94‑1 (MQ=60)

                                                              AGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGG                                 <  SRR4302533.3558910/94‑1 (MQ=60)

                                                              AGGCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGCTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCCATGG                                 >  SRR4302533.3778937/1‑94 (MQ=60)

                                                               GGCATTTCTGGCGCTTACATGCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGC                                <  SRR4302533.1283505/94‑1 (MQ=60)

                                                               GGCATTTCTGCCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGC                                <  SRR4302533.1852856/94‑1 (MQ=60)

                                                               GGCATTGCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGC                                <  SRR4302533.2679652/94‑1 (MQ=60)

                                                                GCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCG                               >  SRR4302533.1149896/1‑94 (MQ=60)

                                                                GCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCG                               <  SRR4302533.5237371/94‑1 (MQ=60)

                                                                GCATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCG                               <  SRR4302533.6167055/94‑1 (MQ=60)

                                                                  ATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTA                             >  SRR4302533.336141/1‑94 (MQ=60)

                                                                  ATTTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTA                             <  SRR4302533.3034175/94‑1 (MQ=60)

                                                                    TTCTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGT                           >  SRR4302533.2490686/1‑94 (MQ=60)

                                                                      CTGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCG                         <  SRR4302533.4150687/94‑1 (MQ=60)

                                                                       TGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGA                        >  SRR4302533.5473129/1‑94 (MQ=60)

                                                                       TGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGA                        <  SRR4302533.1868024/94‑1 (MQ=60)

                                                                       TGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGA                        >  SRR4302533.3813029/1‑94 (MQ=60)

                                                                       TGGCGCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGA                        >  SRR4302533.3453622/1‑94 (MQ=60)

                                                                           GCTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTC                    >  SRR4302533.4813128/1‑94 (MQ=60)

                                                                            CTTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCT                   <  SRR4302533.1519585/94‑1 (MQ=60)

                                                                             TTACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTT                  <  SRR4302533.1211710/94‑1 (MQ=60)

                                                                              TACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTT                 <  SRR4302533.4783426/94‑1 (MQ=60)

                                                                               ACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTC                <  SRR4302533.6310043/94‑1 (MQ=60)

                                                                               ACATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTC                >  SRR4302533.2409640/1‑94 (MQ=60)

                                                                                CATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCT               >  SRR4302533.2648336/1‑94 (MQ=60)

                                                                                 ATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTG              >  SRR4302533.87025/1‑94 (MQ=60)

                                                                                 ATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTG              >  SRR4302533.608571/1‑94 (MQ=60)

                                                                                 ATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTG              >  SRR4302533.5755853/1‑94 (MQ=60)

                                                                                 ATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTG              >  SRR4302533.5438185/1‑94 (MQ=60)

                                                                                 ATTCCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGATGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTG              >  SRR4302533.2060603/1‑94 (MQ=60)

                                                                                    CCGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGAC           <  SRR4302533.4579947/94‑1 (MQ=60)

                                                                                     CGGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACG          >  SRR4302533.1702063/1‑94 (MQ=60)

                                                                                      GGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGG         >  SRR4302533.3118502/1‑94 (MQ=60)

                                                                                      GGGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGG         <  SRR4302533.1755737/94‑1 (MQ=60)

                                                                                       GGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGTC        <  SRR4302533.5980903/94‑1 (MQ=60)

                                                                                       GGCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGC        <  SRR4302533.427078/94‑1 (MQ=60)

                                                                                       GGCAAGCCTCAAAGCTTTCGCGGTGGTTTTACGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGC        <  SRR4302533.5648518/94‑1 (MQ=60)

                                                                                        GCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCG       <  SRR4302533.1631975/94‑1 (MQ=60)

                                                                                        GCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCG       >  SRR4302533.3770937/1‑94 (MQ=60)

                                                                                        GCAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCG       <  SRR4302533.2440936/94‑1 (MQ=60)

                                                                                         CAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGA      >  SRR4302533.3080532/1‑94 (MQ=60)

                                                                                         CAAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGA      >  SRR4302533.5553180/1‑94 (MQ=60)

                                                                                          AAGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAA     >  SRR4302533.1161049/1‑94 (MQ=60)

                                                                                           AGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAA    <  SRR4302533.1754550/94‑1 (MQ=60)

                                                                                           AGCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAA    >  SRR4302533.2174128/1‑94 (MQ=60)

                                                                                            GCCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAG   <  SRR4302533.3897773/94‑1 (MQ=60)

                                                                                             CCTCAAAGCTTTCGCGGCGGTTTTCCGCGCGGCTGTCGCCGTTGCGCTTAAGCAGCGCAATTGCGTAGTCGATCTCTTTCTGGACGGTGAAAGG  >  SRR4302533.5380613/1‑94 (MQ=60)

AAATTGCTCATCAGATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGG  >  NC_000913/2842223‑2842409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`