Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,373	T→C	Y41C (TAC→TGC)	hycE ←	large subunit of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,373	0	T	C	99.5%	662.8 / NA	205	Y41C (TAC→TGC)	hycE	large subunit of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (92/112); total (93/112)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCG  >  NC_000913/2844283‑2844462

tGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGgcaggcag                                                                                         >  1:3064905/1‑93 (MQ=255)

tGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGgcaggcag                                                                                         >  1:2565577/1‑93 (MQ=255)

 gACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGt                                                                                        <  1:2853859/93‑1 (MQ=255)

 gACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGt                                                                                        <  2:1066069/93‑1 (MQ=255)

   ccATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTc                                                                                      <  2:3171624/93‑1 (MQ=255)

   ccATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTc                                                                                      <  1:1584345/93‑1 (MQ=255)

   ccATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTc                                                                                      >  2:2846446/1‑93 (MQ=255)

     aTTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCAc                                                                                    >  2:5204264/1‑93 (MQ=255)

      ttCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCAcc                                                                                   <  2:1340063/93‑1 (MQ=255)

      ttCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCAcc                                                                                   >  1:1993810/1‑93 (MQ=255)

        cAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCtt                                                                                 >  2:4631368/1‑93 (MQ=255)

        cAGTTTGCGTTCGTCGTTACAAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCtt                                                                                 >  1:2587383/1‑93 (MQ=255)

         aGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCttt                                                                                <  2:1836876/93‑1 (MQ=255)

         aGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCttt                                                                                >  2:5752829/1‑93 (MQ=255)

          gTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTa                                                                               >  1:305895/1‑93 (MQ=255)

          gTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTa                                                                               <  1:5256136/93‑1 (MQ=255)

          gTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTa                                                                               <  1:3927339/93‑1 (MQ=255)

           tttGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAc                                                                              <  2:797852/93‑1 (MQ=255)

           tttGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAc                                                                              >  1:1494845/1‑93 (MQ=255)

            ttGCGTTCTTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGTCAGTTCACCTTTACg                                                                             <  2:2682372/93‑1 (MQ=255)

            ttGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACg                                                                             <  1:3901805/93‑1 (MQ=255)

             tGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACgg                                                                            <  1:696568/93‑1 (MQ=255)

             tGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACgg                                                                            <  1:3167393/93‑1 (MQ=255)

              gCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGt                                                                           <  2:1908129/93‑1 (MQ=255)

              gCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGt                                                                           <  1:5943849/93‑1 (MQ=255)

              gCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGt                                                                           <  1:195384/93‑1 (MQ=255)

                gTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGa                                                                         <  1:991744/93‑1 (MQ=255)

                 ttCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGAc                                                                        >  2:5874117/1‑93 (MQ=255)

                  tcgtcgTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACa                                                                       >  2:3923050/1‑93 (MQ=255)

                  tcgtcgTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACa                                                                       >  1:5415365/1‑93 (MQ=255)

                   cgtcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAg                                                                      <  1:1697334/93‑1 (MQ=255)

                    gtcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGt                                                                     <  1:739478/93‑1 (MQ=255)

                     tcgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTc                                                                    >  1:6248494/1‑93 (MQ=255)

                      cgtTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCa                                                                   <  1:5363765/93‑1 (MQ=255)

                         tACCAAAGAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCt                                                                <  2:4009984/93‑1 (MQ=255)

                          aCCAAACAGCACCGACAGTCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTg                                                               >  2:5586286/1‑93 (MQ=255)

                          aCCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTg                                                               <  2:6291826/93‑1 (MQ=255)

                          aCCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTg                                                               <  2:4075902/93‑1 (MQ=255)

                           ccAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGa                                                              <  2:6252801/93‑1 (MQ=255)

                            cAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGAt                                                             >  1:5648345/1‑93 (MQ=255)

                             aaaCAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATc                                                            <  2:1860484/93‑1 (MQ=255)

                                cAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCttt                                                         <  2:5440289/93‑1 (MQ=255)

                                 aGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTg                                                        >  2:177997/1‑93 (MQ=255)

                                 aGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTg                                                        <  1:4460774/93‑1 (MQ=255)

                                 aGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTg                                                        >  2:2826431/1‑93 (MQ=255)

                                  gCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTgg                                                       >  1:2712986/1‑93 (MQ=255)

                                    atcGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTc                                                     <  2:421612/91‑1 (MQ=255)

                                    aCCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTc                                                     >  1:4804853/1‑93 (MQ=255)

                                      cGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTg                                                   >  1:1777012/1‑93 (MQ=255)

                                       gACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCTCCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGc                                                  <  2:26243/93‑1 (MQ=255)

                                       gACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGc                                                  >  2:3971149/1‑93 (MQ=255)

                                       gACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGc                                                  >  2:1125331/1‑93 (MQ=255)

                                       gACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGc                                                  <  2:2962567/93‑1 (MQ=255)

                                        aCAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGcc                                                 >  2:4103717/1‑93 (MQ=255)

                                          cgccagccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAg                                               <  1:4538892/92‑1 (MQ=255)

                                          agccagccACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAg                                               <  2:209249/93‑1 (MQ=255)

                                           gccagccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAgg                                              >  2:2339009/1‑93 (MQ=255)

                                             cagccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCg                                            <  2:3410004/93‑1 (MQ=255)

                                               gccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTg                                          <  2:2857082/93‑1 (MQ=255)

                                               gccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTg                                          >  2:3792675/1‑93 (MQ=255)

                                               gccaCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTg                                          >  1:3240761/1‑93 (MQ=255)

                                                 cgcccTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGt                                        >  1:6086611/3‑93 (MQ=255)

                                                 caCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGt                                        <  2:2903467/93‑1 (MQ=255)

                                                 caCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGt                                        >  1:3624741/1‑93 (MQ=255)

                                                  aCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTc                                       <  1:4749974/93‑1 (MQ=255)

                                                  aCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTc                                       >  1:5603047/1‑93 (MQ=255)

                                                   cccTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTcc                                      >  1:3736091/1‑93 (MQ=255)

                                                    ccTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCa                                     >  1:782112/1‑93 (MQ=255)

                                                    ccTGTTGGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCa                                     <  1:1620327/93‑1 (MQ=255)

                                                     cTGATTGTAGTACAGAAACCCCAACACTTCCGGCAGTCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAg                                    <  1:2429678/93‑1 (MQ=255)

                                                      tGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGc                                   >  1:5754300/1‑93 (MQ=255)

                                                          tGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCacga                               >  2:4517356/1‑93 (MQ=255)

                                                           gtagtaAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCacgac                              >  1:1332359/1‑93 (MQ=255)

                                                                aaaGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCg                         >  2:5302922/1‑93 (MQ=255)

                                                                  aGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGa                       <  2:35045/93‑1 (MQ=255)

                                                                  aGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGa                       >  2:3860859/1‑93 (MQ=255)

                                                                   aaaaCTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGaa                      <  1:4137145/92‑1 (MQ=255)

                                                                     aaCTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAAt                    >  1:3411384/1‑93 (MQ=255)

                                                                     aaCTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAAt                    <  1:3472917/93‑1 (MQ=255)

                                                                        tCCACCACTTCTGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGcc                 <  1:1793226/93‑1 (MQ=255)

                                                                        tCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGcc                 >  1:1049461/1‑93 (MQ=255)

                                                                         ccaccaCTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCt                <  2:3365148/93‑1 (MQ=255)

                                                                         ccaccaCTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCt                <  2:5053229/93‑1 (MQ=255)

                                                                          caccacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTc               <  1:2304590/93‑1 (MQ=255)

                                                                           cccacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCa              <  1:5720835/92‑1 (MQ=255)

                                                                           accacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCa              <  1:1209578/93‑1 (MQ=255)

                                                                           accacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCa              >  2:1072966/1‑93 (MQ=255)

                                                                            ccacATCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAt             <  2:1686976/93‑1 (MQ=255)

                                                                             cacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAtt            <  1:5337983/93‑1 (MQ=255)

                                                                             cacTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAtt            >  2:678513/1‑93 (MQ=255)

                                                                              acTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTc           >  2:4749662/1‑93 (MQ=255)

                                                                              acTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTc           >  2:6082362/1‑93 (MQ=255)

                                                                               cTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCa          <  2:4596503/93‑1 (MQ=255)

                                                                                 tCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgc        <  1:1839035/93‑1 (MQ=255)

                                                                                   cGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcgc      <  2:1588895/93‑1 (MQ=255)

                                                                                   cGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcgc      <  1:872749/93‑1 (MQ=255)

                                                                                   cGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAgcgc      >  1:4269064/1‑93 (MQ=255)

                                                                                    ggcaggcaGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGcgcg     >  1:6134283/1‑93 (MQ=255)

                                                                                      cAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCgcggc   <  2:2756093/93‑1 (MQ=255)

                                                                                       aGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCgcggcg  >  1:4873172/1‑93 (MQ=255)

TGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCG  >  NC_000913/2844283‑2844462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGAT  >  NC_000913/2844280‑2844466

TAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGC                                                                                               <  SRR4302533.3114533/94‑1 (MQ=60)

 AATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCA                                                                                              <  SRR4302533.3298738/94‑1 (MQ=60)

  ATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAG                                                                                             >  SRR4302533.2581365/1‑94 (MQ=60)

  ATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAG                                                                                             >  SRR4302533.5515140/1‑94 (MQ=60)

  ATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAG                                                                                             >  SRR4302533.5884930/1‑94 (MQ=60)

  ATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAG                                                                                             >  SRR4302533.3083730/1‑94 (MQ=60)

   TGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGT                                                                                            <  SRR4302533.3959061/94‑1 (MQ=60)

    GACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTT                                                                                           <  SRR4302533.1589438/94‑1 (MQ=60)

    GACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTT                                                                                           <  SRR4302533.2871417/94‑1 (MQ=60)

      CCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCA                                                                                         <  SRR4302533.1593891/94‑1 (MQ=60)

       CATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCAC                                                                                        >  SRR4302533.5287847/1‑94 (MQ=60)

        ATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACC                                                                                       >  SRR4302533.2006040/1‑94 (MQ=60)

         ATCAGTTTGCCTGCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCT                                                                                      <  SRR4302533.4377518/94‑1 (MQ=60)

          TCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTT                                                                                     <  SRR4302533.82762/94‑1 (MQ=60)

          TCAGTTTGCGTTCGTCGTTACAAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTT                                                                                     >  SRR4302533.2603290/1‑94 (MQ=60)

            AGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTA                                                                                   >  SRR4302533.307764/1‑94 (MQ=60)

            AGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTA                                                                                   <  SRR4302533.1226126/94‑1 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAC                                                                                  <  SRR4302533.3951314/94‑1 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAC                                                                                  <  SRR4302533.5287706/94‑1 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAC                                                                                  <  SRR4302533.4836459/94‑1 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAC                                                                                  >  SRR4302533.3535444/1‑94 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTAC                                                                                  >  SRR4302533.1503852/1‑94 (MQ=60)

             GTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGT‑GTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACG                                                                                 >  SRR4302533.4516652/1‑94 (MQ=60)

              TTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACG                                                                                 <  SRR4302533.5440679/94‑1 (MQ=60)

               TTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGG                                                                                <  SRR4302533.3925630/94‑1 (MQ=60)

                TGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGT                                                                               <  SRR4302533.3186883/94‑1 (MQ=60)

                TGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGT                                                                               <  SRR4302533.700687/94‑1 (MQ=60)

                 GCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTG                                                                              <  SRR4302533.196615/94‑1 (MQ=60)

                 GCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTG                                                                              <  SRR4302533.3002486/94‑1 (MQ=60)

                 GCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTG                                                                              <  SRR4302533.5979796/94‑1 (MQ=60)

                  CGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGA                                                                             <  SRR4302533.1756521/94‑1 (MQ=60)

                  CGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGA                                                                             >  SRR4302533.2585312/1‑94 (MQ=60)

                   GTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGAC                                                                            <  SRR4302533.997541/94‑1 (MQ=60)

                    TTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACA                                                                           <  SRR4302533.263791/94‑1 (MQ=60)

                    TTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACA                                                                           >  SRR4302533.5447930/1‑94 (MQ=60)

                      CGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGT                                                                         <  SRR4302533.1707616/94‑1 (MQ=60)

                      CGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGT                                                                         >  SRR4302533.5769460/1‑94 (MQ=60)

                      CGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGT                                                                         <  SRR4302533.329840/94‑1 (MQ=60)

                       GTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTC                                                                        >  SRR4302533.6285882/1‑94 (MQ=60)

                       GTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTC                                                                        <  SRR4302533.743831/94‑1 (MQ=60)

                        TCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCA                                                                       >  SRR4302533.3482404/1‑94 (MQ=60)

                         CGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAG                                                                      >  SRR4302533.6139138/1‑94 (MQ=60)

                         CGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAG                                                                      <  SRR4302533.5395986/94‑1 (MQ=60)

                         CGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAG                                                                      >  SRR4302533.660763/1‑94 (MQ=60)

                         CGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAG                                                                      <  SRR4302533.5754797/94‑1 (MQ=60)

                         CGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAG                                                                      >  SRR4302533.320962/1‑94 (MQ=60)

                          GTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGC                                                                     >  SRR4302533.1144482/1‑94 (MQ=60)

                          GTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGC                                                                     >  SRR4302533.6254581/1‑94 (MQ=60)

                          GTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGC                                                                     >  SRR4302533.1977506/1‑94 (MQ=60)

                           TTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCT                                                                    <  SRR4302533.1847094/94‑1 (MQ=60)

                           TTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCT                                                                    <  SRR4302533.3950396/94‑1 (MQ=60)

                           TTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCT                                                                    >  SRR4302533.1895504/1‑94 (MQ=60)

                              CCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGAT                                                                 >  SRR4302533.5682492/1‑94 (MQ=60)

                                   CAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTG                                                            <  SRR4302533.1394919/94‑1 (MQ=60)

                                    AGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGG                                                           >  SRR4302533.2729646/1‑94 (MQ=60)

                                    AGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGG                                                           <  SRR4302533.4487756/94‑1 (MQ=60)

                                    AGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGG                                                           <  SRR4302533.2235794/94‑1 (MQ=60)

                                      CACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTC                                                         >  SRR4302533.4833950/1‑94 (MQ=60)

                                       ACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCT                                                        >  SRR4302533.2431006/1‑94 (MQ=60)

                                        CCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTG                                                       <  SRR4302533.615735/94‑1 (MQ=60)

                                        CCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTG                                                       >  SRR4302533.1787830/1‑94 (MQ=60)

                                             CGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGG                                                  <  SRR4302533.4566372/94‑1 (MQ=60)

                                             AGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGG                                                  <  SRR4302533.3152052/94‑1 (MQ=60)

                                                CAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGT                                               <  SRR4302533.2504622/94‑1 (MQ=60)

                                                 AGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTG                                              >  SRR4302533.3260730/1‑94 (MQ=60)

                                                 AGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTG                                              >  SRR4302533.778102/1‑94 (MQ=60)

                                                   CCGCCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGT                                            >  SRR4302533.6123408/1‑94 (MQ=60)

                                                   CCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGT                                            >  SRR4302533.3647045/1‑94 (MQ=60)

                                                    CACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTC                                           >  SRR4302533.5636903/1‑94 (MQ=60)

                                                     ACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCC                                          >  SRR4302533.3759009/1‑94 (MQ=60)

                                                     ACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCC                                          <  SRR4302533.4778773/94‑1 (MQ=60)

                                                      CCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCA                                         >  SRR4302533.786714/1‑94 (MQ=60)

                                                      CCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCA                                         >  SRR4302533.3080816/1‑94 (MQ=60)

                                                       CCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAG                                        >  SRR4302533.1633454/1‑94 (MQ=60)

                                                       CCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAG                                        >  SRR4302533.5048201/1‑94 (MQ=60)

                                                       CCTGTTGGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAG                                        <  SRR4302533.1630143/94‑1 (MQ=60)

                                                        CTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGC                                       >  SRR4302533.5789021/1‑94 (MQ=60)

                                                        CTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGC                                       <  SRR4302533.4881699/94‑1 (MQ=60)

                                                        CTGATTGTAGTACAGAAACCCCAACACTTCCGGCAGTCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGC                                       <  SRR4302533.2444697/94‑1 (MQ=60)

                                                           TTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACG                                    <  SRR4302533.3839977/94‑1 (MQ=60)

                                                           TTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGAACG                                    <  SRR4302533.838841/94‑1 (MQ=60)

                                                            TTGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGA                                   >  SRR4302533.1612362/1‑94 (MQ=60)

                                                             TGTAGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGAC                                  >  SRR4302533.1340355/1‑94 (MQ=60)

                                                                AGTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCC                               >  SRR4302533.71498/1‑94 (MQ=60)

                                                                 GTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCC                              <  SRR4302533.4791901/94‑1 (MQ=60)

                                                                 GTAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCC                              >  SRR4302533.95999/1‑94 (MQ=60)

                                                                  TAAAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCG                             >  SRR4302533.4292540/1‑94 (MQ=60)

                                                                    AAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGA                           >  SRR4302533.3735937/1‑94 (MQ=60)

                                                                    AAGAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGA                           >  SRR4302533.5562721/1‑94 (MQ=60)

                                                                      AAAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAA                         <  SRR4302533.4162249/94‑1 (MQ=60)

                                                                       AAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAAT                        >  SRR4302533.3432454/1‑94 (MQ=60)

                                                                       AAACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAAT                        <  SRR4302533.5660277/94‑1 (MQ=60)

                                                                        AACTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATG                       <  SRR4302533.3494347/94‑1 (MQ=60)

                                                                          CTCCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCC                     >  SRR4302533.1055630/1‑94 (MQ=60)

                                                                           TCCACCACTTCTGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCT                    <  SRR4302533.1804138/94‑1 (MQ=60)

                                                                            CCACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTC                   <  SRR4302533.2855546/94‑1 (MQ=60)

                                                                             CACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCA                  <  SRR4302533.2318866/94‑1 (MQ=60)

                                                                              CCCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAT                 <  SRR4302533.5755376/94‑1 (MQ=60)

                                                                              ACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAT                 >  SRR4302533.1803545/1‑94 (MQ=60)

                                                                              ACCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCAT                 <  SRR4302533.1216841/94‑1 (MQ=60)

                                                                               CCACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATT                <  SRR4302533.4075098/94‑1 (MQ=60)

                                                                                CACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTC               >  SRR4302533.1678985/1‑94 (MQ=60)

                                                                                CACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTC               <  SRR4302533.5369988/94‑1 (MQ=60)

                                                                                CACTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTC               <  SRR4302533.347257/94‑1 (MQ=60)

                                                                                  CTTCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAG             <  SRR4302533.276415/94‑1 (MQ=60)

                                                                                    TCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCG           <  SRR4302533.1850251/94‑1 (MQ=60)

                                                                                    TCCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCG           <  SRR4302533.5382729/94‑1 (MQ=60)

                                                                                     CCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGC          <  SRR4302533.4764646/94‑1 (MQ=60)

                                                                                     CCGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGC          >  SRR4302533.4294762/1‑94 (MQ=60)

                                                                                      CGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCG         <  SRR4302533.877872/94‑1 (MQ=60)

                                                                                      CGGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCG         >  SRR4302533.6171246/1‑94 (MQ=60)

                                                                                       GGCAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGG        >  SRR4302533.6064092/1‑94 (MQ=60)

                                                                                         CAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCG      <  SRR4302533.2269039/94‑1 (MQ=60)

                                                                                         CAGGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCG      >  SRR4302533.4902699/1‑94 (MQ=60)

                                                                                           GGCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAG    <  SRR4302533.2120319/94‑1 (MQ=60)

                                                                                            GCAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGA   >  SRR4302533.746003/1‑94 (MQ=60)

                                                                                             CAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGAT  <  SRR4302533.2569687/94‑1 (MQ=60)

                                                                                             CAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGAT  <  SRR4302533.195548/94‑1 (MQ=60)

TAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCACCTTTACGGTGACAGTCAGCTGATCTTTGGTCTGCCAGGCGTGGTCCAGCACGACGCCCGGAAATGCCTCATTCAGCGCGGCGAGAT  >  NC_000913/2844280‑2844466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`