Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,900,198	G→A	G94D (GGC→GAC)	ygcE →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,900,198	0	G	A	100.0%	881.4 / NA	241	G94D (GGC→GAC)	ygcE	putative kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (121/120); total (121/120)

Only 100 of 251 total aligned reads displayed.

TGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACC  >  NC_000913/2900108‑2900286

tgatgaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACa                                                                                        <  2:5258826/93‑1 (MQ=255)

tgatgaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACa                                                                                        <  2:3648416/93‑1 (MQ=255)

 gatgaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGacac                                                                                       >  2:840573/1‑93 (MQ=255)

 gatgaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGacac                                                                                       >  2:5562532/1‑93 (MQ=255)

  atgaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACg                                                                                      >  1:592257/1‑93 (MQ=255)

    gaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGcc                                                                                    <  1:454935/93‑1 (MQ=255)

    gaGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGcc                                                                                    >  1:180697/1‑93 (MQ=255)

    gaGTCAGTTTGCAGAGAATAAAGAAGATATTGTCGGTATTGGTCTGGGAACCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGcc                                                                                    <  1:2713571/93‑1 (MQ=255)

     aGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCct                                                                                   <  1:4586193/93‑1 (MQ=255)

      gTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCctg                                                                                  <  1:1661912/93‑1 (MQ=255)

       tCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCctgc                                                                                 >  2:1958743/1‑93 (MQ=255)

       tCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCctgc                                                                                 >  1:5186563/1‑93 (MQ=255)

         aGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCctgctg                                                                               <  2:1868636/93‑1 (MQ=255)

         aGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCctgctg                                                                               <  2:5749252/93‑1 (MQ=255)

          gTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCtgctgc                                                                              >  1:1993603/1‑93 (MQ=255)

           tttGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTgcg                                                                             <  2:6059926/93‑1 (MQ=255)

               ccGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGt                                                                         <  1:716462/93‑1 (MQ=255)

               ccGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGt                                                                         <  1:396778/93‑1 (MQ=255)

                 gggAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTg                                                                       <  1:1253160/93‑1 (MQ=255)

                  ggAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGa                                                                      <  2:3574941/93‑1 (MQ=255)

                  ggAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGa                                                                      >  2:3149358/1‑93 (MQ=255)

                  ggAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGa                                                                      >  2:4673065/1‑93 (MQ=255)

                   gAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGAt                                                                     <  1:5495902/93‑1 (MQ=255)

                    aaTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGAtt                                                                    <  2:2691776/93‑1 (MQ=255)

                    aaTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGAtt                                                                    >  2:4482465/1‑93 (MQ=255)

                     aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTa                                                                   <  1:6053999/93‑1 (MQ=255)

                     aTAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACACCTGCTGCGCCGTTGATTa                                                                   <  2:340618/93‑1 (MQ=255)

                      tAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAg                                                                  >  1:3196437/1‑93 (MQ=255)

                      tAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAg                                                                  >  2:4413111/1‑93 (MQ=255)

                       aaaGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGc                                                                 <  2:4226947/93‑1 (MQ=255)

                       aaaGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGc                                                                 >  1:1390850/1‑93 (MQ=255)

                        aagaagATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCt                                                                >  1:5062037/1‑93 (MQ=255)

                        aagaagATATGGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCt                                                                <  1:4098489/93‑1 (MQ=255)

                          gaagaTATTGTCGGGATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTgg                                                              >  2:3506491/1‑93 (MQ=255)

                           aagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGc                                                             <  1:2106531/93‑1 (MQ=255)

                            agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCa                                                            <  1:5218029/93‑1 (MQ=255)

                            agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCa                                                            >  1:4889594/1‑93 (MQ=255)

                              atatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAgg                                                          <  2:4542451/93‑1 (MQ=255)

                              atatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAgg                                                          <  1:1485014/93‑1 (MQ=255)

                               tatTGTCGTTATTGGTCTGGGATCCATCCGTTGCTGCGGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGa                                                         <  2:730783/93‑1 (MQ=255)

                               tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGa                                                         <  2:1902091/93‑1 (MQ=255)

                               tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGa                                                         >  1:574780/1‑93 (MQ=255)

                                atTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGAt                                                        <  1:5601843/93‑1 (MQ=255)

                                  tGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGc                                                      <  1:3304018/93‑1 (MQ=255)

                                  tGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGAGCCGTTGATTAGCTGGCAGGATGc                                                      >  1:1152485/1‑93 (MQ=255)

                                     cGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCAcg                                                   <  1:3669503/93‑1 (MQ=255)

                                      ggTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCAcgc                                                  >  1:3469406/1‑93 (MQ=255)

                                       gTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCAcgcg                                                 >  1:3568703/1‑93 (MQ=255)

                                         aTTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGtt                                               >  1:5441590/1‑93 (MQ=255)

                                            ggTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTaca                                            >  1:4703760/1‑93 (MQ=255)

                                            ggTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTaca                                            <  2:6001991/93‑1 (MQ=255)

                                               cTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGc                                         <  1:5105598/93‑1 (MQ=255)

                                                tGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGcc                                        <  2:239624/93‑1 (MQ=255)

                                                 gggATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGccc                                       <  2:4160380/93‑1 (MQ=255)

                                                 gggATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGccc                                       >  1:4317430/1‑93 (MQ=255)

                                                  ggATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCt                                      >  2:539553/1‑93 (MQ=255)

                                                   gATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCtt                                     <  1:2610366/93‑1 (MQ=255)

                                                    atccatccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTa                                    <  1:2347023/93‑1 (MQ=255)

                                                      ccatccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACg                                  >  2:5783412/1‑93 (MQ=255)

                                                       catccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGa                                 <  1:5884400/93‑1 (MQ=255)

                                                        atccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGaa                                >  2:1186091/1‑93 (MQ=255)

                                                        atccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTGGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGaa                                <  2:1721290/93‑1 (MQ=255)

                                                        atccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGATGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGaa                                >  2:4371424/1‑93 (MQ=255)

                                                         tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                               >  2:5195652/1‑93 (MQ=255)

                                                         tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                               >  2:4020884/1‑93 (MQ=255)

                                                         tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                               >  1:6148095/1‑93 (MQ=255)

                                                         tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                               <  1:2549637/93‑1 (MQ=255)

                                                         tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                               >  1:3039428/1‑93 (MQ=255)

                                                           cGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacac                             <  1:5333870/93‑1 (MQ=255)

                                                            gTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacaca                            <  1:4405732/93‑1 (MQ=255)

                                                            gTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacaca                            >  2:628867/1‑93 (MQ=255)

                                                            cttGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacaca                            <  1:5810534/92‑1 (MQ=255)

                                                             ttGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAacacac                           >  1:3079759/1‑93 (MQ=255)

                                                              tgctgcCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACt                          <  2:333203/93‑1 (MQ=255)

                                                                 tgcCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAc                       >  2:2260130/1‑93 (MQ=255)

                                                                  gcCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAcc                      >  2:3685669/1‑93 (MQ=255)

                                                                   ccGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc                     <  1:1359037/93‑1 (MQ=255)

                                                                    cGTGCGTTATTGAAAGCCGATGACACGTCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTCCGATCACACTAACCCt                    >  1:2172611/1‑93 (MQ=255)

                                                                    cGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                    <  1:2315023/93‑1 (MQ=255)

                                                                    cGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                    <  2:3009789/93‑1 (MQ=255)

                                                                     gTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                   >  2:3896530/1‑93 (MQ=255)

                                                                     gTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                   <  1:4816786/93‑1 (MQ=255)

                                                                      tGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTGCACGCCCTTACGAACACACTAACCCTGa                  >  1:637829/1‑93 (MQ=255)

                                                                      tGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                  <  1:4167929/93‑1 (MQ=255)

                                                                      tGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                  >  2:142088/1‑93 (MQ=255)

                                                                        cGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg                >  2:1528284/1‑93 (MQ=255)

                                                                        cGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg                <  1:1519872/93‑1 (MQ=255)

                                                                          ttATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTg              >  1:4305099/1‑93 (MQ=255)

                                                                            aTTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGc            >  2:490026/1‑93 (MQ=255)

                                                                            aTTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGc            >  2:780774/1‑93 (MQ=255)

                                                                             ttGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa           >  1:3115253/1‑93 (MQ=255)

                                                                              tGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCat          <  1:948720/93‑1 (MQ=255)

                                                                               gAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCata         <  1:5675617/93‑1 (MQ=255)

                                                                               gAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCata         >  1:1820842/1‑93 (MQ=255)

                                                                                aaaGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat        <  2:5305255/93‑1 (MQ=255)

                                                                                  aGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGt      >  2:318425/1‑93 (MQ=255)

                                                                                     cGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAc   <  1:1556921/93‑1 (MQ=255)

                                                                                      gATGACCCGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACTCGTTACTCGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc  <  1:164751/93‑1 (MQ=255)

                                                                                      gATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc  >  2:5866800/1‑93 (MQ=255)

                                                                                      gATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc  >  2:4609890/1‑93 (MQ=255)

TGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACC  >  NC_000913/2900108‑2900286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  >  NC_000913/2900105‑2900291

ATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGA                                                                                               >  SRR4302533.244502/1‑94 (MQ=60)

ATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGGATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGA                                                                                               >  SRR4302533.2741887/1‑94 (MQ=60)

 TTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGAC                                                                                              <  SRR4302533.1153872/94‑1 (MQ=60)

 TTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGAC                                                                                              <  SRR4302533.3510770/94‑1 (MQ=60)

  TTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACA                                                                                             <  SRR4302533.5554787/94‑1 (MQ=60)

   TGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACAC                                                                                            >  SRR4302533.5501864/1‑94 (MQ=60)

   TGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACAC                                                                                            <  SRR4302533.1488110/94‑1 (MQ=60)

   TGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTCCGTTATTGAAAGCCGATGACAC                                                                                            <  SRR4302533.5225634/94‑1 (MQ=60)

    GATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACG                                                                                           >  SRR4302533.595755/1‑94 (MQ=60)

     ATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGC                                                                                          >  SRR4302533.5791152/1‑94 (MQ=60)

      TGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCC                                                                                         >  SRR4302533.181823/1‑94 (MQ=60)

       GAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCT                                                                                        <  SRR4302533.457653/94‑1 (MQ=60)

       GAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCT                                                                                        <  SRR4302533.4594180/94‑1 (MQ=60)

       GAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCT                                                                                        <  SRR4302533.3213558/94‑1 (MQ=60)

       GAGTCAGTTTGCAGAGAATAAAGAAGATATTGTCGGTATTGGTCTGGGAACCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCT                                                                                        <  SRR4302533.2730235/94‑1 (MQ=60)

        AGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTG                                                                                       <  SRR4302533.4613974/94‑1 (MQ=60)

         GTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGC                                                                                      >  SRR4302533.5217870/1‑94 (MQ=60)

         GTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGC                                                                                      >  SRR4302533.1992978/1‑94 (MQ=60)

         GTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGC                                                                                      <  SRR4302533.1671959/94‑1 (MQ=60)

          TCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCT                                                                                     <  SRR4302533.1511462/94‑1 (MQ=60)

          TCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCT                                                                                     <  SRR4302533.4342038/94‑1 (MQ=60)

           CAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTG                                                                                    >  SRR4302533.765212/1‑94 (MQ=60)

           CAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTG                                                                                    >  SRR4302533.5395775/1‑94 (MQ=60)

            AGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGC                                                                                   <  SRR4302533.1535542/94‑1 (MQ=60)

            AGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGC                                                                                   >  SRR4302533.2005832/1‑94 (MQ=60)

                  CCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTT                                                                             <  SRR4302533.399178/94‑1 (MQ=60)

                  CCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTT                                                                             <  SRR4302533.720686/94‑1 (MQ=60)

                  CCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTT                                                                             >  SRR4302533.1557795/1‑94 (MQ=60)

                  CCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCAGACGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTT                                                                             >  SRR4302533.4474876/1‑94 (MQ=60)

                   CGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTG                                                                            >  SRR4302533.2399935/1‑94 (MQ=60)

                    GGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGA                                                                           <  SRR4302533.1260654/94‑1 (MQ=60)

                     GGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGAT                                                                          <  SRR4302533.3573723/94‑1 (MQ=60)

                      GAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATT                                                                         >  SRR4302533.2579484/1‑94 (MQ=60)

                      GAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATT                                                                         <  SRR4302533.5529050/94‑1 (MQ=60)

                      GAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATT                                                                         >  SRR4302533.6171634/1‑94 (MQ=60)

                       AATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTA                                                                        <  SRR4302533.4649792/94‑1 (MQ=60)

                        ATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAG                                                                       >  SRR4302533.4764192/1‑94 (MQ=60)

                        ATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAG                                                                       >  SRR4302533.1830599/1‑94 (MQ=60)

                        ATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAG                                                                       <  SRR4302533.6090663/94‑1 (MQ=60)

                        ATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAG                                                                       >  SRR4302533.3216106/1‑94 (MQ=60)

                         TAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGC                                                                      >  SRR4302533.1399227/1‑94 (MQ=60)

                         TAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGC                                                                      >  SRR4302533.3113342/1‑94 (MQ=60)

                          AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCT                                                                     <  SRR4302533.4967210/94‑1 (MQ=60)

                          AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCT                                                                     >  SRR4302533.5092623/1‑94 (MQ=60)

                          AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCT                                                                     >  SRR4302533.3638839/1‑94 (MQ=60)

                          AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCT                                                                     >  SRR4302533.1416129/1‑94 (MQ=60)

                           AAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTG                                                                    <  SRR4302533.5626927/94‑1 (MQ=60)

                           AAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTG                                                                    >  SRR4302533.42982/1‑94 (MQ=60)

                           AAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTG                                                                    >  SRR4302533.1332230/1‑94 (MQ=60)

                           AAGAAGATATGGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTG                                                                    <  SRR4302533.4123435/94‑1 (MQ=60)

                            AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGG                                                                   >  SRR4302533.5699468/1‑94 (MQ=60)

                            AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGG                                                                   >  SRR4302533.5807385/1‑94 (MQ=60)

                              AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                 >  SRR4302533.5482158/1‑94 (MQ=60)

                              AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                 <  SRR4302533.2119521/94‑1 (MQ=60)

                              AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                 >  SRR4302533.4919223/1‑94 (MQ=60)

                              AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                 >  SRR4302533.6232651/1‑94 (MQ=60)

                              AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                 >  SRR4302533.6076066/1‑94 (MQ=60)

                               AGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAG                                                                <  SRR4302533.5249439/94‑1 (MQ=60)

                               AGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAG                                                                >  SRR4302533.3324303/1‑94 (MQ=60)

                                GATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGG                                                               >  SRR4302533.2693253/1‑94 (MQ=60)

                                 ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGA                                                              >  SRR4302533.578172/1‑94 (MQ=60)

                                 ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGA                                                              <  SRR4302533.1493976/94‑1 (MQ=60)

                                 ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGA                                                              <  SRR4302533.5166153/94‑1 (MQ=60)

                                   ATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATG                                                            >  SRR4302533.4103088/1‑94 (MQ=60)

                                   ATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATG                                                            <  SRR4302533.5635693/94‑1 (MQ=60)

                                   ATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATG                                                            >  SRR4302533.2348731/1‑94 (MQ=60)

                                    TTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGC                                                           <  SRR4302533.601051/94‑1 (MQ=60)

                                    TTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGAGCCGTTGATTAGCTGGCAGGATGC                                                           >  SRR4302533.1159387/1‑94 (MQ=60)

                                     TGTCGGTATTGGTCTGGGATCCATTCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGGTGCGCCGTTGATTAGCTGGCAGGATGCA                                                          >  SRR4302533.3253218/1‑94 (MQ=60)

                                     TGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCA                                                          <  SRR4302533.3324422/94‑1 (MQ=60)

                                      GTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCAC                                                         <  SRR4302533.180001/94‑1 (MQ=60)

                                        CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAATCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGC                                                       >  SRR4302533.4111582/1‑94 (MQ=60)

                                        CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGC                                                       <  SRR4302533.3692060/94‑1 (MQ=60)

                                        CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGC                                                       >  SRR4302533.3490823/1‑94 (MQ=60)

                                        CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGC                                                       >  SRR4302533.5016205/1‑94 (MQ=60)

                                         GGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCG                                                      >  SRR4302533.3590699/1‑94 (MQ=60)

                                         GGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCG                                                      <  SRR4302533.317932/94‑1 (MQ=60)

                                         GGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCG                                                      >  SRR4302533.5113690/1‑94 (MQ=60)

                                          GTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGT                                                     >  SRR4302533.166040/1‑94 (MQ=60)

                                           TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTT                                                    >  SRR4302533.5474365/1‑94 (MQ=60)

                                           TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTT                                                    >  SRR4302533.2122832/1‑94 (MQ=60)

                                           TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTT                                                    >  SRR4302533.5694929/1‑94 (MQ=60)

                                            ATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTA                                                   >  SRR4302533.632922/1‑94 (MQ=60)

                                              TGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACA                                                 >  SRR4302533.4732273/1‑94 (MQ=60)

                                               GGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACAC                                                >  SRR4302533.5944813/1‑94 (MQ=60)

                                                  ccggtATCCATCCGTTGCTGCCGTGCGTTATTGAAATCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCC                                             <  SRR4302533.5728017/89‑1 (MQ=60)

                                                  CTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCC                                             >  SRR4302533.1667789/1‑94 (MQ=60)

                                                  CTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCC                                             >  SRR4302533.595346/1‑94 (MQ=60)

                                                  CTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCC                                             <  SRR4302533.5136440/94‑1 (MQ=60)

                                                   TGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCC                                            <  SRR4302533.488410/94‑1 (MQ=60)

                                                   TGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCC                                            >  SRR4302533.4343429/1‑94 (MQ=60)

                                                     GGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTT                                          <  SRR4302533.181843/94‑1 (MQ=60)

                                                     GGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTT                                          <  SRR4302533.3026842/94‑1 (MQ=60)

                                                      GATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTA                                         <  SRR4302533.2626390/94‑1 (MQ=60)

                                                      GATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTA                                         <  SRR4302533.4307325/94‑1 (MQ=60)

                                                       ATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTAC                                        <  SRR4302533.2361547/94‑1 (MQ=60)

                                                       ATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTAC                                        <  SRR4302533.5343434/94‑1 (MQ=60)

                                                        TCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACG                                       <  SRR4302533.5260659/94‑1 (MQ=60)

                                                          CATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAA                                     <  SRR4302533.5919959/94‑1 (MQ=60)

                                                          CATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAA                                     >  SRR4302533.338513/1‑94 (MQ=60)

                                                           ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                    >  SRR4302533.3058029/1‑94 (MQ=60)

                                                           ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                    <  SRR4302533.3069680/94‑1 (MQ=60)

                                                           ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                    >  SRR4302533.6185108/1‑94 (MQ=60)

                                                            TCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACA                                   >  SRR4302533.3091264/1‑94 (MQ=60)

                                                            TCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACA                                   <  SRR4302533.2565331/94‑1 (MQ=60)

                                                             CCGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACAC                                  <  SRR4302533.3902347/94‑1 (MQ=60)

                                                              CGTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACA                                 <  SRR4302533.5365839/94‑1 (MQ=60)

                                                              CGTTGCTGCCGTGCGTTATTGAAAGACGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGGACGCGTTACACGCCCTTACGAACACA                                 >  SRR4302533.2314870/1‑94 (MQ=60)

                                                               GTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                <  SRR4302533.5891421/94‑1 (MQ=60)

                                                               GTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                <  SRR4302533.413369/94‑1 (MQ=60)

                                                               GTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                <  SRR4302533.4432338/94‑1 (MQ=60)

                                                               GTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                >  SRR4302533.3098657/1‑94 (MQ=60)

                                                               GTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                >  SRR4302533.3632317/1‑94 (MQ=60)

                                                               CTTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                <  SRR4302533.5845621/94‑1 (MQ=60)

                                                                TTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACT                               <  SRR4302533.4833151/94‑1 (MQ=60)

                                                                TTGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACT                               >  SRR4302533.4579258/1‑94 (MQ=60)

                                                                 TGCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTA                              >  SRR4302533.683504/1‑94 (MQ=60)

                                                                  GCTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAA                             >  SRR4302533.2070165/1‑94 (MQ=60)

                                                                   CTGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAC                            <  SRR4302533.1626944/94‑1 (MQ=60)

                                                                    TGCCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACC                           >  SRR4302533.1307309/1‑94 (MQ=60)

                                                                      CCGTGCGTTATTGAAAGCCGATGACACGTCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTCCGATCACACTAACCCT                         >  SRR4302533.2185970/1‑94 (MQ=60)

                                                                      CCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                         >  SRR4302533.2401704/1‑94 (MQ=60)

                                                                      CCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                         <  SRR4302533.1367206/94‑1 (MQ=60)

                                                                      CCGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                         <  SRR4302533.4349180/94‑1 (MQ=60)

                                                                       CGTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTG                        <  SRR4302533.2329362/94‑1 (MQ=60)

                                                                        GTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTGCACGCCCTTACGAACACACTAACCCTGA                       >  SRR4302533.641581/1‑94 (MQ=60)

                                                                        GTGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGA                       <  SRR4302533.4845981/94‑1 (MQ=60)

                                                                         TGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                      <  SRR4302533.1371002/94‑1 (MQ=60)

                                                                         TGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                      <  SRR4302533.210904/94‑1 (MQ=60)

                                                                         TGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                      <  SRR4302533.4193187/94‑1 (MQ=60)

                                                                         TGCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                      <  SRR4302533.1168229/94‑1 (MQ=60)

                                                                          GCGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACG                     <  SRR4302533.2650912/94‑1 (MQ=60)

                                                                           CGTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGT                    <  SRR4302533.1529022/94‑1 (MQ=60)

                                                                            GTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTG                   >  SRR4302533.4331001/1‑94 (MQ=60)

                                                                            GTTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTG                   >  SRR4302533.518604/1‑94 (MQ=60)

                                                                             TTATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTTG                  <  SRR4302533.4401636/94‑1 (MQ=60)

                                                                               ATTGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCA                >  SRR4302533.3134409/1‑94 (MQ=60)

                                                                                 TGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATA              <  SRR4302533.954293/94‑1 (MQ=60)

                                                                                 TGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATA              >  SRR4302533.946204/1‑94 (MQ=60)

                                                                                 TGAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATA              >  SRR4302533.1831964/1‑94 (MQ=60)

                                                                                  GAAAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATAT             <  SRR4302533.5709895/94‑1 (MQ=60)

                                                                                    AAGCCGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGT           >  SRR4302533.5761581/1‑94 (MQ=60)

                                                                                        CGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACC       <  SRR4302533.1566292/94‑1 (MQ=60)

                                                                                        CGATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACC       >  SRR4302533.3385346/1‑94 (MQ=60)

                                                                                         GATGACCCGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACTCGTTACTCGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCT      <  SRR4302533.165798/94‑1 (MQ=60)

                                                                                         GATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCT      <  SRR4302533.222513/94‑1 (MQ=60)

                                                                                          ATGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTC     >  SRR4302533.500451/1‑94 (MQ=60)

                                                                                           TGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCT    >  SRR4302533.1023008/1‑94 (MQ=60)

                                                                                           TGACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCT    <  SRR4302533.5925910/94‑1 (MQ=60)

                                                                                             ACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  >  SRR4302533.5670322/1‑94 (MQ=60)

                                                                                             ACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  <  SRR4302533.5140771/94‑1 (MQ=60)

                                                                                             ACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  >  SRR4302533.1239340/1‑94 (MQ=60)

                                                                                             ACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  <  SRR4302533.2819594/94‑1 (MQ=60)

                                                                                             ACACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  >  SRR4302533.813664/1‑94 (MQ=60)

ATTTGATGAGTCAGTTTGCCGGGAATAAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT  >  NC_000913/2900105‑2900291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`