Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,930,115 G→A G308G (GGC→GGT)  fucO ← L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,930,1150GA99.6% 824.0 / NA 231G308G (GGC→GGT) fucOL‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (116/114);  minor base C (0/1);  total (116/115)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACGCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAA  >  NC_000913/2930025‑2930203
                                                                                          |                                                                                        
tgGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACAcc                                                                                        <  1:4310861/93‑1 (MQ=255)
  gCGGAATACCGACATCACGGTTGAGTGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCa                                                                                      <  1:6085476/93‑1 (MQ=255)
  gCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCa                                                                                      >  2:2894828/1‑93 (MQ=255)
  gCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCa                                                                                      <  1:2518882/93‑1 (MQ=255)
   cGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAt                                                                                     <  1:79394/93‑1 (MQ=255)
   cGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAt                                                                                     <  1:2666438/93‑1 (MQ=255)
    ggAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATa                                                                                    >  2:3918175/1‑93 (MQ=255)
    ggAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATa                                                                                    >  2:5975870/1‑93 (MQ=255)
    ggAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATa                                                                                    <  1:2693515/93‑1 (MQ=255)
     gAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATaa                                                                                   <  1:4430075/93‑1 (MQ=255)
     gAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATaa                                                                                   <  2:314545/93‑1 (MQ=255)
     gAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATaa                                                                                   <  2:6131972/93‑1 (MQ=255)
        tACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgc                                                                                >  1:3519837/1‑93 (MQ=255)
          ccGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgc                                                                              >  1:5064824/1‑93 (MQ=255)
           cGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcg                                                                             >  1:2388413/1‑93 (MQ=255)
            gACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcgc                                                                            <  1:1698220/93‑1 (MQ=255)
            gACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcgc                                                                            >  1:3003462/1‑93 (MQ=255)
             aCATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcgcg                                                                           >  1:4480199/1‑93 (MQ=255)
             aCATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcgcg                                                                           >  2:1303750/1‑93 (MQ=255)
             aCATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAcgcgcgcg                                                                           >  1:327320/1‑93 (MQ=255)
              cATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGa                                                                          >  2:4316174/1‑93 (MQ=255)
               aTCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGat                                                                         >  2:1594084/1‑93 (MQ=255)
                tCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGata                                                                        <  1:3080182/93‑1 (MQ=255)
                tCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGata                                                                        <  1:4070799/93‑1 (MQ=255)
                  aCGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATc                                                                      >  1:1787949/1‑93 (MQ=255)
                   cGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATcg                                                                     <  2:819847/93‑1 (MQ=255)
                    ggTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATcgc                                                                    <  1:1005134/93‑1 (MQ=255)
                    ggTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATcgc                                                                    <  1:5762881/93‑1 (MQ=255)
                       tGAGAGCAAACACCGCTGCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGt                                                                 <  2:2704772/93‑1 (MQ=255)
                        gagaGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTa                                                                <  2:3655327/93‑1 (MQ=255)
                        gagaGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTa                                                                >  1:2550111/1‑93 (MQ=255)
                           agCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACtt                                                             >  2:73516/1‑93 (MQ=255)
                              aaaCACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTtctc                                                          <  1:5607642/93‑1 (MQ=255)
                              aaaCACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTtctc                                                          >  2:5188335/1‑93 (MQ=255)
                               aaCACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCa                                                         <  1:5894853/93‑1 (MQ=255)
                               aaCACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCa                                                         >  1:2110604/1‑93 (MQ=255)
                               aaCACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCa                                                         >  2:1752408/1‑93 (MQ=255)
                                acacCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCAc                                                        >  2:408281/1‑93 (MQ=255)
                                acacCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCAc                                                        <  2:5722950/93‑1 (MQ=255)
                                   ccGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCgg                                                     >  1:953744/1‑93 (MQ=255)
                                   ccGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCgg                                                     <  2:5240017/93‑1 (MQ=255)
                                     gCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTa                                                   >  1:2002532/1‑93 (MQ=255)
                                       ttCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTaaa                                                 <  1:3861145/93‑1 (MQ=255)
                                        tCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTTCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAg                                                >  1:1279549/1‑93 (MQ=255)
                                         cAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGt                                               >  2:4709626/1‑93 (MQ=255)
                                          aaCAGCGGCATTACGCGCCTCTTCTAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTc                                              <  2:3976482/93‑1 (MQ=255)
                                           aCAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCa                                             >  1:4937341/1‑93 (MQ=255)
                                                ggCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGtt                                        >  1:1357377/1‑93 (MQ=255)
                                                ggCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGtt                                        >  1:1738948/1‑93 (MQ=255)
                                                   aTTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTtata                                     >  1:4350629/1‑93 (MQ=255)
                                                   aTTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTtata                                     <  1:3398988/93‑1 (MQ=255)
                                                    ttACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAACGTCAGCGTTATaa                                    >  1:4038495/1‑93 (MQ=255)
                                                    ttACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATaa                                    <  2:1780011/93‑1 (MQ=255)
                                                     tACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAAc                                   >  2:262544/1‑93 (MQ=255)
                                                     tACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAAc                                   >  2:147193/1‑93 (MQ=255)
                                                       cgcgCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGc                                 <  1:3139693/93‑1 (MQ=255)
                                                        gcgcCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCa                                <  1:3591121/93‑1 (MQ=255)
                                                           ccTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGa                             >  1:1656278/1‑93 (MQ=255)
                                                           ccTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGa                             >  2:1149634/1‑93 (MQ=255)
                                                            ctctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGAc                            >  2:6046433/1‑93 (MQ=255)
                                                             tctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACa                           >  2:1226603/1‑93 (MQ=255)
                                                             tctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACa                           >  1:2035807/1‑93 (MQ=255)
                                                             tctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACa                           >  1:1498249/1‑93 (MQ=255)
                                                              ctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACAt                          >  2:5351990/1‑93 (MQ=255)
                                                              ctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACAt                          >  2:351537/1‑93 (MQ=255)
                                                              ctTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACAt                          <  1:6139485/93‑1 (MQ=255)
                                                               ttCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATg                         >  1:1429442/1‑93 (MQ=255)
                                                               ttCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATg                         >  2:2211212/1‑93 (MQ=255)
                                                               ttCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATg                         <  2:79223/93‑1 (MQ=255)
                                                               tgcgaGGCTCATGCCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATg                         <  1:2776060/89‑1 (MQ=255)
                                                                tCCAGTCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGc                        <  2:4354538/93‑1 (MQ=255)
                                                                  cAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCgg                      <  1:1922046/93‑1 (MQ=255)
                                                                   aGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGt                     <  1:691135/93‑1 (MQ=255)
                                                                   aGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGt                     >  2:1059530/1‑93 (MQ=255)
                                                                   aGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGt                     <  2:3746501/93‑1 (MQ=255)
                                                                     gCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTaa                   >  1:1857568/1‑93 (MQ=255)
                                                                       tCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACa                 >  2:240521/1‑93 (MQ=255)
                                                                        cATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAg                >  2:4783742/1‑93 (MQ=255)
                                                                        cATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAg                <  2:946448/93‑1 (MQ=255)
                                                                         aTACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAgg               >  2:55640/1‑93 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              <  2:3351445/93‑1 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              >  2:861600/1‑93 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              <  1:2237115/93‑1 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              <  1:5028068/93‑1 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              <  2:5055022/93‑1 (MQ=255)
                                                                          tACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGa              <  2:2839100/93‑1 (MQ=255)
                                                                           aCCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGAt             >  2:320242/1‑93 (MQ=255)
                                                                            ccTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATg            <  2:1954643/93‑1 (MQ=255)
                                                                            ccTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATg            >  1:3413928/1‑93 (MQ=255)
                                                                             cTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATgg           <  2:563896/93‑1 (MQ=255)
                                                                             cTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATgg           <  1:3691302/93‑1 (MQ=255)
                                                                              ttCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGc          >  2:1373025/1‑93 (MQ=255)
                                                                              ttCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGc          >  1:4145373/1‑93 (MQ=255)
                                                                               tCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCg         >  1:5447566/1‑93 (MQ=255)
                                                                                ccACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGt        >  2:3699128/1‑93 (MQ=255)
                                                                                 cACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGtt       <  1:1022604/93‑1 (MQ=255)
                                                                                  aCTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTc      <  1:4619501/93‑1 (MQ=255)
                                                                                  aCTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTc      >  1:2530989/1‑93 (MQ=255)
                                                                                    tttCACCCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGc    <  1:5410640/93‑1 (MQ=255)
                                                                                      tCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCaa  >  1:1959822/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACGCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAA  >  NC_000913/2930025‑2930203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACGCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACACCG  >  NC_000913/2930023‑2930208
                                                                                            |                                                                                             
TGTGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACAC                                                                                              <  SRR4302533.471484/94‑1 (MQ=60)
TGTGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACAC                                                                                              >  SRR4302533.1037811/1‑94 (MQ=60)
  TGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCC                                                                                            <  SRR4302533.4336811/94‑1 (MQ=60)
  TGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCC                                                                                            >  SRR4302533.3853935/1‑94 (MQ=60)
    GCGGAATACCGACATCACGGTTGAGTGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAT                                                                                          <  SRR4302533.6122267/94‑1 (MQ=60)
    GCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAT                                                                                          <  SRR4302533.2534403/94‑1 (MQ=60)
    GCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAT                                                                                          <  SRR4302533.2534404/94‑1 (MQ=60)
    GCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCAT                                                                                          <  SRR4302533.4440020/94‑1 (MQ=60)
     CGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATA                                                                                         <  SRR4302533.79901/94‑1 (MQ=60)
     CGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATA                                                                                         <  SRR4302533.791505/94‑1 (MQ=60)
     CGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATA                                                                                         <  SRR4302533.2556593/94‑1 (MQ=60)
     CGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATA                                                                                         <  SRR4302533.2682798/94‑1 (MQ=60)
      GGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAA                                                                                        <  SRR4302533.2710017/94‑1 (MQ=60)
      GGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAA                                                                                        <  SRR4302533.2684666/94‑1 (MQ=60)
       GAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAC                                                                                       <  SRR4302533.191619/94‑1 (MQ=60)
       GAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAC                                                                                       <  SRR4302533.1290545/94‑1 (MQ=60)
       GAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAAC                                                                                       <  SRR4302533.4456854/94‑1 (MQ=60)
         ATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGC                                                                                     >  SRR4302533.3541536/1‑94 (MQ=60)
         ATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGC                                                                                     >  SRR4302533.1310248/1‑94 (MQ=60)
          TACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCG                                                                                    <  SRR4302533.1015779/94‑1 (MQ=60)
           ACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGC                                                                                   >  SRR4302533.5095418/1‑94 (MQ=60)
            CCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCG                                                                                  >  SRR4302533.2403168/1‑94 (MQ=60)
             CGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGC                                                                                 >  SRR4302533.3021832/1‑94 (MQ=60)
              GACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCG                                                                                <  SRR4302533.1708510/94‑1 (MQ=60)
              GACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCG                                                                                <  SRR4302533.4339167/94‑1 (MQ=60)
              GACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCG                                                                                >  SRR4302533.181076/1‑94 (MQ=60)
              GACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCG                                                                                >  SRR4302533.329310/1‑94 (MQ=60)
              GACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCG                                                                                >  SRR4302533.4507303/1‑94 (MQ=60)
               ACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGA                                                                               >  SRR4302533.315907/1‑94 (MQ=60)
                CATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGAT                                                                              >  SRR4302533.1680228/1‑94 (MQ=60)
                 ATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGGATTACGCGCCTCTTCCAGGCTCATTCCTTCCACTTTCACACCCATAACGCGCGCGATA                                                                             <  SRR4302533.613937/94‑1 (MQ=60)
                 ATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATA                                                                             >  SRR4302533.3560271/1‑94 (MQ=60)
                  TCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATAT                                                                            >  SRR4302533.2659435/1‑94 (MQ=60)
                  TCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATAT                                                                            <  SRR4302533.3099080/94‑1 (MQ=60)
                  TCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATAT                                                                            <  SRR4302533.4095581/94‑1 (MQ=60)
                   CACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATC                                                                           >  SRR4302533.1798816/1‑94 (MQ=60)
                   CACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATC                                                                           >  SRR4302533.3686164/1‑94 (MQ=60)
                   CACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATC                                                                           <  SRR4302533.6330386/94‑1 (MQ=60)
                      GGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCG                                                                        <  SRR4302533.5797660/94‑1 (MQ=60)
                      GGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCG                                                                        <  SRR4302533.1011012/94‑1 (MQ=60)
                       GTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCGACTTTCACACCCATAACGCGCGCGATATCGCGG                                                                       >  SRR4302533.1770480/1‑94 (MQ=60)
                       GTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGG                                                                       >  SRR4302533.3845748/1‑94 (MQ=60)
                       GTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGG                                                                       <  SRR4302533.3465068/94‑1 (MQ=60)
                         TGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTA                                                                     >  SRR4302533.2565807/1‑94 (MQ=60)
                          GAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTAC                                                                    <  SRR4302533.891454/94‑1 (MQ=60)
                          GAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTAC                                                                    >  SRR4302533.2021249/1‑94 (MQ=60)
                             AGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTC                                                                 <  SRR4302533.1727259/94‑1 (MQ=60)
                               CAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTC                                                               >  SRR4302533.6033000/1‑94 (MQ=60)
                               CAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTC                                                               >  SRR4302533.1652387/1‑94 (MQ=60)
                               CAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTC                                                               <  SRR4302533.5475581/94‑1 (MQ=60)
                                AAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCA                                                              >  SRR4302533.2123621/1‑94 (MQ=60)
                                AAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCA                                                              <  SRR4302533.2489525/94‑1 (MQ=60)
                                AAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCA                                                              >  SRR4302533.3423088/1‑94 (MQ=60)
                                AAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCA                                                              <  SRR4302533.5641526/94‑1 (MQ=60)
                                AAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCA                                                              >  SRR4302533.2349187/1‑94 (MQ=60)
                                 AACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCAC                                                             <  SRR4302533.5930454/94‑1 (MQ=60)
                                 AACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCAC                                                             >  SRR4302533.3040498/1‑94 (MQ=60)
                                  ACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACC                                                            <  SRR4302533.4562920/94‑1 (MQ=60)
                                    ACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGG                                                          >  SRR4302533.959348/1‑94 (MQ=60)
                                      CGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTA                                                        >  SRR4302533.2014867/1‑94 (MQ=60)
                                       GCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAA                                                       >  SRR4302533.1998969/1‑94 (MQ=60)
                                         TTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTTCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAG                                                     >  SRR4302533.1287241/1‑94 (MQ=60)
                                         TTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAG                                                     <  SRR4302533.1392040/94‑1 (MQ=60)
                                         TTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAG                                                     >  SRR4302533.4829439/1‑94 (MQ=60)
                                         TTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAG                                                     <  SRR4302533.3884713/94‑1 (MQ=60)
                                            AACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCA                                                  >  SRR4302533.4967233/1‑94 (MQ=60)
                                             ACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAG                                                 <  SRR4302533.5279848/94‑1 (MQ=60)
                                              CAGCGGCATTACGCGCCTCTTCCGGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGC                                                <  SRR4302533.3118092/94‑1 (MQ=60)
                                               AGCGGCATTACGCGCCTCTTCCAGTCTCATACCTTCCACTTTCACACCCATAACGCGCTCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCG                                               <  SRR4302533.1097232/94‑1 (MQ=60)
                                               AGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCG                                               >  SRR4302533.5900687/1‑94 (MQ=60)
                                                 CGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTT                                             >  SRR4302533.1365535/1‑94 (MQ=60)
                                                 CGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTT                                             >  SRR4302533.1749495/1‑94 (MQ=60)
                                                  GGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTA                                            >  SRR4302533.3269964/1‑94 (MQ=60)
                                                    CATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATA                                          >  SRR4302533.4376883/1‑94 (MQ=60)
                                                     ATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAACGTCAGCGTTATAA                                         >  SRR4302533.4063069/1‑94 (MQ=60)
                                                     ATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAA                                         <  SRR4302533.3419978/94‑1 (MQ=60)
                                                     ATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAA                                         >  SRR4302533.1200801/1‑94 (MQ=60)
                                                         CGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCA                                     <  SRR4302533.3158994/94‑1 (MQ=60)
                                                          GCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCAT                                    <  SRR4302533.544176/94‑1 (MQ=60)
                                                          GCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCAT                                    <  SRR4302533.3613228/94‑1 (MQ=60)
                                                          GCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCAT                                    <  SRR4302533.4449995/94‑1 (MQ=60)
                                                          GCGCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCAT                                    >  SRR4302533.4224535/1‑94 (MQ=60)
                                                            GCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGA                                  <  SRR4302533.3536627/94‑1 (MQ=60)
                                                            GCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGA                                  >  SRR4302533.1666301/1‑94 (MQ=60)
                                                            GCCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGA                                  <  SRR4302533.5998126/94‑1 (MQ=60)
                                                             CCTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGAC                                 <  SRR4302533.49955/94‑1 (MQ=60)
                                                              CTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACA                                >  SRR4302533.2048361/1‑94 (MQ=60)
                                                              CTCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACA                                >  SRR4302533.1507276/1‑94 (MQ=60)
                                                               TCTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACAT                               <  SRR4302533.2871422/94‑1 (MQ=60)
                                                                CTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATG                              >  SRR4302533.330809/1‑94 (MQ=60)
                                                                CTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATG                              <  SRR4302533.1965417/94‑1 (MQ=60)
                                                                CTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATG                              >  SRR4302533.1438084/1‑94 (MQ=60)
                                                                CTTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATG                              <  SRR4302533.6176465/94‑1 (MQ=60)
                                                                 tgcgAGGCTCATGCCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGC                             <  SRR4302533.2793111/90‑1 (MQ=60)
                                                                 TTCCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGC                             >  SRR4302533.1763226/1‑94 (MQ=60)
                                                                   CCAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGG                           >  SRR4302533.2658270/1‑94 (MQ=60)
                                                                    CAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGT                          >  SRR4302533.4159864/1‑94 (MQ=60)
                                                                    CAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGT                          <  SRR4302533.1508434/94‑1 (MQ=60)
                                                                    CAGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGT                          <  SRR4302533.1933777/94‑1 (MQ=60)
                                                                     AGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCTCCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTA                         <  SRR4302533.2092047/94‑1 (MQ=60)
                                                                     AGGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTA                         <  SRR4302533.695220/94‑1 (MQ=60)
                                                                      GGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAA                        >  SRR4302533.2489949/1‑94 (MQ=60)
                                                                      GGCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAA                        >  SRR4302533.1868897/1‑94 (MQ=60)
                                                                       GCTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAAC                       >  SRR4302533.970604/1‑94 (MQ=60)
                                                                        CTCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACA                      >  SRR4302533.2994371/1‑94 (MQ=60)
                                                                         TCATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAG                     <  SRR4302533.5724469/94‑1 (MQ=60)
                                                                           ATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGA                   <  SRR4302533.4146893/94‑1 (MQ=60)
                                                                           ATACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGA                   >  SRR4302533.4868828/1‑94 (MQ=60)
                                                                            TACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGAT                  <  SRR4302533.5058515/94‑1 (MQ=60)
                                                                            TACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGAT                  <  SRR4302533.2063550/94‑1 (MQ=60)
                                                                            TACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGAT                  <  SRR4302533.2250943/94‑1 (MQ=60)
                                                                             ACCTTCCACTTTCGCACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATG                 <  SRR4302533.2171188/94‑1 (MQ=60)
                                                                             ACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATG                 >  SRR4302533.3435021/1‑94 (MQ=60)
                                                                             ACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATG                 >  SRR4302533.2736316/1‑94 (MQ=60)
                                                                             ACCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATG                 >  SRR4302533.5612905/1‑94 (MQ=60)
                                                                              CCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGG                >  SRR4302533.392015/1‑94 (MQ=60)
                                                                              CCTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGG                <  SRR4302533.1441142/94‑1 (MQ=60)
                                                                               CTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGC               <  SRR4302533.3713981/94‑1 (MQ=60)
                                                                               CTTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGC               >  SRR4302533.4170496/1‑94 (MQ=60)
                                                                                TTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCG              >  SRR4302533.5480383/1‑94 (MQ=60)
                                                                                TTCCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCG              <  SRR4302533.1953738/94‑1 (MQ=60)
                                                                                  CCACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTT            <  SRR4302533.740784/94‑1 (MQ=60)
                                                                                   CACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTC           <  SRR4302533.1028616/94‑1 (MQ=60)
                                                                                   CACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTC           >  SRR4302533.2546571/1‑94 (MQ=60)
                                                                                   CACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTC           >  SRR4302533.4072574/1‑94 (MQ=60)
                                                                                    ACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCG          <  SRR4302533.5080165/94‑1 (MQ=60)
                                                                                    ACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCG          <  SRR4302533.4647495/94‑1 (MQ=60)
                                                                                    ACTTTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCG          <  SRR4302533.446667/94‑1 (MQ=60)
                                                                                      TTTCACCCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCA        <  SRR4302533.5443171/94‑1 (MQ=60)
                                                                                       TTCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAA       >  SRR4302533.1971783/1‑94 (MQ=60)
                                                                                        TCACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCACC      >  SRR4302533.5687500/1‑94 (MQ=60)
                                                                                          ACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACAC    >  SRR4302533.3686336/1‑94 (MQ=60)
                                                                                          ACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACAC    >  SRR4302533.998161/1‑94 (MQ=60)
                                                                                          ACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACAC    <  SRR4302533.5673968/94‑1 (MQ=60)
                                                                                          ACACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACAC    >  SRR4302533.2380450/1‑94 (MQ=60)
                                                                                           CACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACACC   >  SRR4302533.4248550/1‑94 (MQ=60)
                                                                                           CACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACACC   >  SRR4302533.4220252/1‑94 (MQ=60)
                                                                                            CCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGCCGTTCGCAACACCG  <  SRR4302533.3349704/94‑1 (MQ=60)
                                                                                            ACCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACACCG  <  SRR4302533.5502927/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGTGGCGGAATACCGACATCACGGTTGAGAGCAAACACCGCTTCAACAGCGGCATTACGCGCCTCTTCCAGGCTCATACCTTCCACTTTCACGCCCATAACGCGCGCGATATCGCGGTACTTCTCACCGGTAAAGTCAGCGTTATAACGCATGACATGCGGTAACAGGATGGCGTTCGCAACACCG  >  NC_000913/2930023‑2930208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑