Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,935,409 T→C intergenic (+28/‑51) fucI → / → fucK L‑fucose isomerase/L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,935,4090TC100.0% 862.0 / NA 268intergenic (+28/‑51)fucI/fucKL‑fucose isomerase/L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (130/138);  total (130/138)

Only 100 of 273 total aligned reads displayed.

ATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAA  >  NC_000913/2935319‑2935498
                                                                                          |                                                                                         
 tGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGc                                                                                        >  2:2427473/1‑93 (MQ=255)
 tGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGc                                                                                        <  1:4082405/93‑1 (MQ=255)
 tGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGc                                                                                        >  1:897476/1‑93 (MQ=255)
 tGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTA‑GGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCt                                                                                       >  1:1706065/1‑93 (MQ=255)
    atatTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTcc                                                                                     >  2:358937/1‑93 (MQ=255)
         gAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTa                                                                                >  2:669433/1‑93 (MQ=255)
         gAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTa                                                                                >  1:5077875/1‑93 (MQ=255)
          aaGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTAc                                                                               <  1:4221574/93‑1 (MQ=255)
           aGGCTAGGATTACCGCGCTTGCCAGAACTACGGTCCGTCGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTAcc                                                                              <  2:4610670/93‑1 (MQ=255)
           aGGCCAGGATTACCGCGCTTGCCAGACCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTAcc                                                                              <  1:2094446/93‑1 (MQ=255)
            ggCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCg                                                                             <  2:3895242/93‑1 (MQ=255)
            ggCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCg                                                                             <  2:2005860/93‑1 (MQ=255)
            ggCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCg                                                                             <  2:3297769/93‑1 (MQ=255)
                 ggATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCt                                                                        >  1:5756350/1‑93 (MQ=255)
                 ggATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCt                                                                        >  1:3265373/1‑93 (MQ=255)
                 ggATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCt                                                                        >  2:1049033/1‑93 (MQ=255)
                  gATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTg                                                                       >  2:1534348/1‑93 (MQ=255)
                   aTTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGa                                                                      <  1:3058523/93‑1 (MQ=255)
                    ttACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGaa                                                                     <  2:4811363/93‑1 (MQ=255)
                      aCCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAca                                                                   >  1:4363461/1‑93 (MQ=255)
                       ccGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaa                                                                  <  1:3692353/93‑1 (MQ=255)
                       ccGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaa                                                                  >  2:3512066/1‑93 (MQ=255)
                        cgcgCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaag                                                                 <  2:4703269/93‑1 (MQ=255)
                           gcTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaagcaa                                                              >  1:261049/1‑93 (MQ=255)
                           gcTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaagcaa                                                              <  1:5329917/93‑1 (MQ=255)
                            cTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAcaagcaag                                                             <  2:2682957/93‑1 (MQ=255)
                               gCCAGACCTCCGGTCCGTTGTACAAGCGTTAATACATTCCTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGt                                                          <  2:1315320/93‑1 (MQ=255)
                               gCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGt                                                          <  1:4578181/93‑1 (MQ=255)
                                ccAGAACTACGGTCCGTGGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTg                                                         <  2:2206624/93‑1 (MQ=255)
                                 cAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGAgg                                                        >  2:4346358/1‑93 (MQ=255)
                                  aGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGt                                                       >  1:2932389/1‑93 (MQ=255)
                                   gAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGtt                                                      <  1:272330/93‑1 (MQ=255)
                                   gAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGtt                                                      <  2:254959/93‑1 (MQ=255)
                                   gAACTACGGTCCGTTGTACAAGCGTTAATACATAACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGtt                                                      <  1:2237997/93‑1 (MQ=255)
                                    aaCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTa                                                     <  1:5238684/93‑1 (MQ=255)
                                    aaCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTa                                                     <  2:317772/93‑1 (MQ=255)
                                    aaCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTa                                                     <  1:999584/93‑1 (MQ=255)
                                    aaCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTa                                                     <  2:3411342/93‑1 (MQ=255)
                                    aaCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTa                                                     <  2:1627332/93‑1 (MQ=255)
                                     aCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTat                                                    >  2:425703/1‑92 (MQ=255)
                                     aCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAg                                                    <  2:5471510/93‑1 (MQ=255)
                                      cTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGc                                                   <  2:2047549/93‑1 (MQ=255)
                                        aCGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCg                                                 <  2:1572798/93‑1 (MQ=255)
                                        aCGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCg                                                 >  2:3028148/1‑93 (MQ=255)
                                        aCGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCg                                                 >  1:2695926/1‑93 (MQ=255)
                                          ggTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGa                                               <  1:346762/93‑1 (MQ=255)
                                           gTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGAt                                              <  1:5953026/93‑1 (MQ=255)
                                           gTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGAt                                              <  1:3114720/93‑1 (MQ=255)
                                            tCCTTTGTACAAGCGTTAATACATTACTCCATGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATa                                             <  2:6194238/93‑1 (MQ=255)
                                            tCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATa                                             <  2:4351212/93‑1 (MQ=255)
                                            tCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATa                                             <  2:1363639/93‑1 (MQ=255)
                                            tCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATa                                             >  1:2563747/1‑93 (MQ=255)
                                            tCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATa                                             <  1:2783438/93‑1 (MQ=255)
                                             ccGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATaa                                            >  2:744116/1‑93 (MQ=255)
                                              cGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAg                                           >  1:2435505/1‑93 (MQ=255)
                                              cGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCCGCTaaa                                           >  1:1432144/1‑92 (MQ=255)
                                               gTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGc                                          <  2:5284725/93‑1 (MQ=255)
                                               gTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGc                                          <  2:4072130/93‑1 (MQ=255)
                                                ttGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCa                                         >  2:2364490/1‑93 (MQ=255)
                                                     cAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGGAATGtt                                    >  2:4890585/1‑93 (MQ=255)
                                                     cAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGtt                                    >  2:596319/1‑93 (MQ=255)
                                                     cAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGtt                                    >  2:3696565/1‑93 (MQ=255)
                                                      aaGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTa                                   >  2:5215581/1‑93 (MQ=255)
                                                      aaGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATATTa                                   >  1:5937171/1‑93 (MQ=255)
                                                       aGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGGAATGTTAt                                  >  1:6268282/1‑93 (MQ=255)
                                                        gCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATc                                 <  2:1632328/93‑1 (MQ=255)
                                                        gCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATc                                 <  2:2920545/93‑1 (MQ=255)
                                                        gCGTGAGTACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATc                                 <  1:5509510/93‑1 (MQ=255)
                                                         cGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATcc                                <  1:3416867/93‑1 (MQ=255)
                                                         cGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATcc                                <  1:5177129/93‑1 (MQ=255)
                                                            tAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGc                             >  1:1333750/1‑93 (MQ=255)
                                                             aaTACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGGCGGATAAGGAATGTTATTCTGCt                            >  2:5773037/1‑93 (MQ=255)
                                                              aTACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCtt                           >  1:4152401/1‑92 (MQ=255)
                                                              aTACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCta                           >  2:4949098/1‑93 (MQ=255)
                                                               tACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATTCGGCtat                          >  1:503463/1‑93 (MQ=255)
                                                               tACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCtat                          <  2:1841759/93‑1 (MQ=255)
                                                                aCATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCtata                         >  1:3916373/1‑93 (MQ=255)
                                                                 cATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTatat                        >  2:4845951/1‑93 (MQ=255)
                                                                  aTTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATAtt                       <  1:602834/93‑1 (MQ=255)
                                                                    tACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGc                     <  2:5144683/93‑1 (MQ=255)
                                                                     aCTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCa                    <  1:6094497/93‑1 (MQ=255)
                                                                     aCTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGAGAAGCACTGTTATTCGGCTATATTGCa                    >  2:2106312/1‑93 (MQ=255)
                                                                        cccTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGa                 <  2:4567186/93‑1 (MQ=255)
                                                                          cTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTTTATTGCAGGAgg               >  2:1282671/1‑92 (MQ=255)
                                                                            gatgtgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGa             <  2:5058436/93‑1 (MQ=255)
                                                                             atgtgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGAt            >  2:1071560/1‑93 (MQ=255)
                                                                             atgtgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGAt            <  2:838955/93‑1 (MQ=255)
                                                                             atgtgatgCCCGGCCGCTCCGGCTACCAGGCATGAACAGACAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGAt            <  1:6306722/93‑1 (MQ=255)
                                                                               gtgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTa          <  1:1523685/93‑1 (MQ=255)
                                                                                tgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATTCGGCTATATTGCAGGAGCGATTAt         >  2:5336989/1‑93 (MQ=255)
                                                                                tgatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTAt         <  2:1875892/93‑1 (MQ=255)
                                                                                 gatgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTAta        >  2:5713158/1‑92 (MQ=255)
                                                                                  atgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGa       <  2:3097977/93‑1 (MQ=255)
                                                                                  atgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGa       <  2:2731742/93‑1 (MQ=255)
                                                                                   tgCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGaa      >  2:4024330/1‑93 (MQ=255)
                                                                                    gCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGaaa     <  1:1058120/93‑1 (MQ=255)
                                                                                     cccGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAAc    <  2:907219/93‑1 (MQ=255)
                                                                                      ccGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACa   >  1:492287/1‑93 (MQ=255)
                                                                                       cGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACaa  <  1:4412932/93‑1 (MQ=255)
                                                                                       cGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACaa  <  1:1258646/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAA  >  NC_000913/2935319‑2935498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAAG  >  NC_000913/2935316‑2935502
                                                                                             |                                                                                             
GGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGC                                                                                               >  SRR4302533.188364/1‑94 (MQ=60)
 GCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCC                                                                                              <  SRR4302533.4433621/94‑1 (MQ=60)
 GCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCC                                                                                              <  SRR4302533.1703162/94‑1 (MQ=60)
 GCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCC                                                                                              <  SRR4302533.6003194/94‑1 (MQ=60)
  CATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCG                                                                                             <  SRR4302533.1304556/94‑1 (MQ=60)
   ATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGC                                                                                            >  SRR4302533.902744/1‑94 (MQ=60)
   ATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTA‑GGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCT                                                                                           >  SRR4302533.1716399/1‑94 (MQ=60)
    TGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCT                                                                                           <  SRR4302533.4107257/94‑1 (MQ=60)
    TGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCT                                                                                           >  SRR4302533.4688778/1‑94 (MQ=60)
    AGGATATTGAAGGCCAAGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCT                                                                                           <  SRR4302533.4189556/94‑1 (MQ=60)
     GGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTC                                                                                          >  SRR4302533.5105591/1‑94 (MQ=60)
      GATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCC                                                                                         >  SRR4302533.1270313/1‑94 (MQ=60)
       ATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCG                                                                                        <  SRR4302533.4430855/94‑1 (MQ=60)
          TTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCT                                                                                     <  SRR4302533.3421418/94‑1 (MQ=60)
           TGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTA                                                                                    >  SRR4302533.5108565/1‑94 (MQ=60)
           TGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTACCTGATGTGATGCCCGGCCGCTCCGGCTC                                                                                    >  SRR4302533.4332126/1‑94 (MQ=60)
            GAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTAC                                                                                   >  SRR4302533.5796954/1‑94 (MQ=60)
             AAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACC                                                                                  <  SRR4302533.4247102/94‑1 (MQ=60)
              AGGCCAGGATTACCGCGCTTGCCAGACCTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCG                                                                                 <  SRR4302533.2107344/94‑1 (MQ=60)
                GCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGG                                                                               <  SRR4302533.4187621/94‑1 (MQ=60)
                GCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGG                                                                               <  SRR4302533.3186784/94‑1 (MQ=60)
                 CCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGC                                                                              >  SRR4302533.1687708/1‑94 (MQ=60)
                   AGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCT                                                                            >  SRR4302533.6142694/1‑94 (MQ=60)
                   AGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCT                                                                            >  SRR4302533.3285523/1‑94 (MQ=60)
                   AGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCT                                                                            >  SRR4302533.2314196/1‑94 (MQ=60)
                   AGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCT                                                                            >  SRR4302533.5791088/1‑94 (MQ=60)
                    GGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTG                                                                           >  SRR4302533.4531590/1‑94 (MQ=60)
                      ATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAA                                                                         <  SRR4302533.3077291/94‑1 (MQ=60)
                       TTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAC                                                                        <  SRR4302533.2499663/94‑1 (MQ=60)
                       TTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAC                                                                        <  SRR4302533.6212347/94‑1 (MQ=60)
                        TACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACA                                                                       >  SRR4302533.738716/1‑94 (MQ=60)
                        TACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACA                                                                       >  SRR4302533.4389799/1‑94 (MQ=60)
                         ACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAA                                                                      <  SRR4302533.268636/94‑1 (MQ=60)
                         ACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAA                                                                      >  SRR4302533.1596128/1‑94 (MQ=60)
                          CCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAG                                                                     <  SRR4302533.3715039/94‑1 (MQ=60)
                           CGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGC                                                                    >  SRR4302533.5987062/1‑94 (MQ=60)
                             CGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAA                                                                  >  SRR4302533.262632/1‑94 (MQ=60)
                              GCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAG                                                                 <  SRR4302533.5361862/94‑1 (MQ=60)
                                 TGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGT                                                              <  SRR4302533.4946947/94‑1 (MQ=60)
                                  GCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTG                                                             <  SRR4302533.5305002/94‑1 (MQ=60)
                                  GCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTG                                                             <  SRR4302533.1466799/94‑1 (MQ=60)
                                  GCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTG                                                             <  SRR4302533.4605927/94‑1 (MQ=60)
                                   CTAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGG                                                            <  SRR4302533.5125712/94‑1 (MQ=60)
                                   CCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGG                                                            >  SRR4302533.2819421/1‑94 (MQ=60)
                                    CAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGT                                                           >  SRR4302533.2950322/1‑94 (MQ=60)
                                     AGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAAaaaaaaaaaaagggg                                                          >  SRR4302533.6064022/1‑79 (MQ=60)
                                      GAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTA                                                         <  SRR4302533.273986/94‑1 (MQ=60)
                                      GAACTACGGTCCGTTGTACAAGCGTTAATACATAACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTA                                                         <  SRR4302533.2251828/94‑1 (MQ=60)
                                       AACTACGGTCCGTTGTACAATCGTTAATCCATTCCTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAG                                                        <  SRR4302533.2591338/94‑1 (MQ=60)
                                       AACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAG                                                        <  SRR4302533.1005418/94‑1 (MQ=60)
                                       AACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAG                                                        <  SRR4302533.5270183/94‑1 (MQ=60)
                                        ACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGC                                                       >  SRR4302533.1960018/1‑94 (MQ=60)
                                         CTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCC                                                      >  SRR4302533.2883050/1‑94 (MQ=60)
                                         CTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCC                                                      <  SRR4302533.2218530/94‑1 (MQ=60)
                                          TACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCG                                                     >  SRR4302533.2712448/1‑94 (MQ=60)
                                          TACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCG                                                     >  SRR4302533.2768426/1‑94 (MQ=60)
                                           ACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGG                                                    <  SRR4302533.6330119/94‑1 (MQ=60)
                                             GGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGAT                                                  <  SRR4302533.348880/94‑1 (MQ=60)
                                              GTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATA                                                 >  SRR4302533.2579527/1‑94 (MQ=60)
                                              GTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATA                                                 <  SRR4302533.3133872/94‑1 (MQ=60)
                                              GTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATA                                                 >  SRR4302533.3123593/1‑94 (MQ=60)
                                              GTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATA                                                 <  SRR4302533.5989036/94‑1 (MQ=60)
                                               TCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAA                                                <  SRR4302533.559733/94‑1 (MQ=60)
                                               TCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAA                                                <  SRR4302533.2800535/94‑1 (MQ=60)
                                                CCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCcgctaaa                                               >  SRR4302533.1440799/1‑87 (MQ=60)
                                                CCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAG                                               >  SRR4302533.5144073/1‑94 (MQ=60)
                                                CCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAG                                               >  SRR4302533.964461/1‑94 (MQ=60)
                                                CCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAG                                               >  SRR4302533.2450547/1‑94 (MQ=60)
                                                 CGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGC                                              <  SRR4302533.1435097/94‑1 (MQ=60)
                                                 CGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGC                                              <  SRR4302533.3806580/94‑1 (MQ=60)
                                                  GTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCA                                             >  SRR4302533.665749/1‑94 (MQ=60)
                                                       ACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTT                                        <  SRR4302533.3576391/94‑1 (MQ=60)
                                                        CAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTA                                       >  SRR4302533.4994825/1‑94 (MQ=60)
                                                        CAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATATTA                                       >  SRR4302533.5973069/1‑94 (MQ=60)
                                                         AAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGGAATGTTAT                                      >  SRR4302533.6305739/1‑94 (MQ=60)
                                                         AAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTAT                                      <  SRR4302533.5218513/94‑1 (MQ=60)
                                                         AAGCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTAT                                      <  SRR4302533.4089355/94‑1 (MQ=60)
                                                           GCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCC                                    >  SRR4302533.339423/1‑94 (MQ=60)
                                                           GCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCC                                    <  SRR4302533.3224630/94‑1 (MQ=60)
                                                           GCGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCC                                    <  SRR4302533.3925636/94‑1 (MQ=60)
                                                           GCGTGAGTACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCC                                    <  SRR4302533.5542769/94‑1 (MQ=60)
                                                            CGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCG                                   <  SRR4302533.6107375/94‑1 (MQ=60)
                                                            CGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCG                                   <  SRR4302533.5208397/94‑1 (MQ=60)
                                                            CGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCG                                   >  SRR4302533.2020205/1‑94 (MQ=60)
                                                            CGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCG                                   <  SRR4302533.3437978/94‑1 (MQ=60)
                                                            CGTTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCG                                   >  SRR4302533.3466276/1‑94 (MQ=60)
                                                              TTAATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGC                                 >  SRR4302533.1341756/1‑94 (MQ=60)
                                                                AATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTT                               >  SRR4302533.4177560/1‑94 (MQ=60)
                                                                 ATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATTCGGCTAT                              >  SRR4302533.506452/1‑94 (MQ=60)
                                                                 ATACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTAT                              >  SRR4302533.562241/1‑94 (MQ=60)
                                                                  TACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATA                             <  SRR4302533.25052/94‑1 (MQ=60)
                                                                  TACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATA                             >  SRR4302533.3940294/1‑94 (MQ=60)
                                                                   ACATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATAT                            >  SRR4302533.1363198/1‑94 (MQ=60)
                                                                    CATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATT                           <  SRR4302533.4360241/94‑1 (MQ=60)
                                                                     ATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTG                          <  SRR4302533.606377/94‑1 (MQ=60)
                                                                     ATTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTG                          >  SRR4302533.2597247/1‑94 (MQ=60)
                                                                      TTACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGC                         <  SRR4302533.924084/94‑1 (MQ=60)
                                                                        ACTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAG                       <  SRR4302533.6131317/94‑1 (MQ=60)
                                                                         CTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGG                      >  SRR4302533.2228182/1‑94 (MQ=60)
                                                                         CTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGG                      <  SRR4302533.2722373/94‑1 (MQ=60)
                                                                         CTCCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGG                      <  SRR4302533.1272479/94‑1 (MQ=60)
                                                                           CCCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAG                    >  SRR4302533.5197872/1‑94 (MQ=60)
                                                                            CCTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATTCGGCTATATTGCAGGAGC                   >  SRR4302533.5367689/1‑94 (MQ=60)
                                                                             CTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCG                  <  SRR4302533.5265145/94‑1 (MQ=60)
                                                                             CTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCG                  >  SRR4302533.4960887/1‑94 (MQ=60)
                                                                             CTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCG                  <  SRR4302533.3495597/94‑1 (MQ=60)
                                                                             CTGATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCG                  <  SRR4302533.1258953/94‑1 (MQ=60)
                                                                               GATGTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATTCGGCTATATTGCAGGAGCGAT                >  SRR4302533.4498916/1‑94 (MQ=60)
                                                                                ATGTGATGCCCGGCCGCTCCGGCTACCAGGCATGAACAGACAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATT               <  SRR4302533.6344504/94‑1 (MQ=60)
                                                                                  GTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTAT             >  SRR4302533.411878/1‑94 (MQ=60)
                                                                                  GTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTAT             <  SRR4302533.6025362/94‑1 (MQ=60)
                                                                                  GTGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTAT             <  SRR4302533.1532858/94‑1 (MQ=60)
                                                                                   TGATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATG            <  SRR4302533.609247/94‑1 (MQ=60)
                                                                                    GATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGA           <  SRR4302533.5302092/94‑1 (MQ=60)
                                                                                    GATGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGA           <  SRR4302533.3984024/94‑1 (MQ=60)
                                                                                      TGCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAA         >  SRR4302533.2779241/1‑94 (MQ=60)
                                                                                       GCCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAAC        <  SRR4302533.1064345/94‑1 (MQ=60)
                                                                                        CCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCGATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACA       >  SRR4302533.2701381/1‑94 (MQ=60)
                                                                                        CCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACA       <  SRR4302533.5824167/94‑1 (MQ=60)
                                                                                        CCCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACA       >  SRR4302533.495229/1‑94 (MQ=60)
                                                                                         CCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAA      >  SRR4302533.3082808/1‑94 (MQ=60)
                                                                                         CCGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAA      <  SRR4302533.5986061/94‑1 (MQ=60)
                                                                                          CGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAG     <  SRR4302533.303192/94‑1 (MQ=60)
                                                                                          CGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAG     >  SRR4302533.578227/1‑94 (MQ=60)
                                                                                          CGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAG     <  SRR4302533.4439581/94‑1 (MQ=60)
                                                                                          CGGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAG     <  SRR4302533.1266174/94‑1 (MQ=60)
                                                                                           GGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGA    <  SRR4302533.5332038/94‑1 (MQ=60)
                                                                                           GGCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGA    >  SRR4302533.4962579/1‑94 (MQ=60)
                                                                                            GCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAA   >  SRR4302533.5605806/1‑94 (MQ=60)
                                                                                            GCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAA   >  SRR4302533.3362946/1‑94 (MQ=60)
                                                                                            GCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAA   <  SRR4302533.4403858/94‑1 (MQ=60)
                                                                                            GCCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAA   <  SRR4302533.3731195/94‑1 (MQ=60)
                                                                                             CCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAAG  <  SRR4302533.5407450/94‑1 (MQ=60)
                                                                                             CCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAAG  >  SRR4302533.2325866/1‑94 (MQ=60)
                                                                                             CCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAAG  <  SRR4302533.4141973/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGAAG  >  NC_000913/2935316‑2935502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑