Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,953,377 T→C T217A (ACG→GCG)  recB ← RecB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,953,3770TC100.0% 828.8 / NA 257T217A (ACG→GCG) recBRecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (125/132);  total (125/132)

Only 100 of 273 total aligned reads displayed.

TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466
                                                                                          |                                                                                         
 cAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtc                                                                                        >  2:267528/1‑93 (MQ=255)
  aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtca                                                                                       >  2:1373385/1‑93 (MQ=255)
   gcgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcat                                                                                      >  2:337543/1‑93 (MQ=255)
    cgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatc                                                                                     >  2:4130553/1‑93 (MQ=255)
    cgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatc                                                                                     <  2:1063119/93‑1 (MQ=255)
     gcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatca                                                                                    >  2:909512/1‑93 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   <  1:5072281/93‑1 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  2:1644015/1‑93 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   <  1:4489841/93‑1 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  1:1234312/1‑93 (MQ=255)
       ctCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCatcatc                                                                                  <  1:3551203/92‑1 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 >  2:1907260/1‑93 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 >  2:2006837/1‑93 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 >  1:416563/1‑93 (MQ=255)
         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTTCCGTATCAATACGCGCCACAAGTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                <  2:5243355/93‑1 (MQ=255)
         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                >  1:1818948/1‑93 (MQ=255)
         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                >  1:4800395/1‑93 (MQ=255)
         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                <  1:3858796/93‑1 (MQ=255)
          cAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCggg                                                                               <  1:2922351/93‑1 (MQ=255)
           atttCACCCACTGCGTCGCGCCACTTCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGTGCTTCATCATCGggc                                                                              >  2:5050725/3‑93 (MQ=255)
           aGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggc                                                                              <  2:3698984/93‑1 (MQ=255)
           aGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggc                                                                              <  2:4302551/93‑1 (MQ=255)
            gTTCACCCACTGCGTCGCGCGACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcg                                                                             >  2:6064926/1‑93 (MQ=255)
            gTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcg                                                                             <  2:2819460/93‑1 (MQ=255)
               cACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGgcggcg                                                                          <  1:6262413/93‑1 (MQ=255)
               cACCCACTGCGTCGCGCCACTGCTGTTTTAACGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGgcggcg                                                                          >  1:1887081/1‑93 (MQ=255)
                aCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcgg                                                                         <  1:757311/93‑1 (MQ=255)
                 cccACTGCGTCGCGCCACTGCTGTTTTGCCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGt                                                                        <  2:4800651/93‑1 (MQ=255)
                   cACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGc                                                                      <  2:1140624/93‑1 (MQ=255)
                        cGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGa                                                                 <  2:158263/93‑1 (MQ=255)
                         gTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                <  1:3437459/93‑1 (MQ=255)
                         gTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                >  1:1519136/1‑93 (MQ=255)
                         gTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                >  2:2907173/1‑93 (MQ=255)
                          tCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATa                                                               >  1:1675258/1‑93 (MQ=255)
                          tCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATa                                                               <  1:1744110/93‑1 (MQ=255)
                          tCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATa                                                               <  1:3132795/93‑1 (MQ=255)
                           cgcgCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATaa                                                              <  1:2824813/93‑1 (MQ=255)
                            gcgcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAc                                                             <  1:5193967/93‑1 (MQ=255)
                            gcgcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAc                                                             >  2:1193408/1‑93 (MQ=255)
                              gcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCg                                                           >  1:5657188/1‑93 (MQ=255)
                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         <  1:6258693/93‑1 (MQ=255)
                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         <  1:4431247/93‑1 (MQ=255)
                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         <  2:3884040/93‑1 (MQ=255)
                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         >  2:4256298/1‑93 (MQ=255)
                                 aCTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgcg                                                        >  2:4065514/1‑93 (MQ=255)
                                 aCTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgcg                                                        >  2:4580621/1‑93 (MQ=255)
                                   tgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCt                                                      >  2:6205287/1‑93 (MQ=255)
                                    gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCtt                                                     >  1:104414/1‑93 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGc                                                  >  1:24586/1‑93 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGc                                                  >  2:4914725/1‑93 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGc                                                  >  2:5136116/1‑93 (MQ=255)
                                        ttttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGcc                                                 >  1:2065126/1‑93 (MQ=255)
                                         tttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCt                                                <  2:2127540/93‑1 (MQ=255)
                                             ccGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCa                                            <  2:2752921/93‑1 (MQ=255)
                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         >  1:6013870/1‑93 (MQ=255)
                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         >  2:1473637/1‑93 (MQ=255)
                                                  tCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATaa                                       <  1:5022506/93‑1 (MQ=255)
                                                  tCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATaa                                       >  1:3759020/1‑93 (MQ=255)
                                                    aaTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACg                                     >  1:1118079/1‑93 (MQ=255)
                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   <  2:3308605/93‑1 (MQ=255)
                                                       aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAtt                                  <  2:693985/93‑1 (MQ=255)
                                                       aCGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAtt                                  <  2:2843430/93‑1 (MQ=255)
                                                       aCGCGCCACAATTTGCGCGTGACGGGAAACCAGCGCTTCATCATCGGGCGGCTGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAtt                                  <  1:2639798/93‑1 (MQ=255)
                                                        cgcgCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTa                                 >  2:2948925/1‑93 (MQ=255)
                                                        cgcgCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTa                                 >  1:1473284/1‑93 (MQ=255)
                                                         gcgcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTaa                                >  1:4263629/1‑93 (MQ=255)
                                                         gcgcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTaa                                >  1:1310125/1‑93 (MQ=255)
                                                           gcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAata                              >  1:3354124/1‑93 (MQ=255)
                                                             cacaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATc                            >  1:2208042/1‑93 (MQ=255)
                                                             cacaATTGGCGCGGGACGGGAGGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATc                            <  2:385570/93‑1 (MQ=255)
                                                              acaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcg                           >  1:4375086/1‑93 (MQ=255)
                                                               caATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgc                          <  2:5909426/93‑1 (MQ=255)
                                                                 aTTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCgcgc                        >  1:4072158/1‑93 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       >  2:4210549/1‑93 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       <  1:2827260/93‑1 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       <  1:2006837/93‑1 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       <  1:4905241/93‑1 (MQ=255)
                                                                    tGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagc                     <  2:2728770/93‑1 (MQ=255)
                                                                     gcgcGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagca                    >  1:3283352/1‑93 (MQ=255)
                                                                     gcgcGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagca                    >  2:4365387/1‑93 (MQ=255)
                                                                      cgcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCaa                   <  1:3658359/93‑1 (MQ=255)
                                                                      cgcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCaa                   >  1:5677591/1‑93 (MQ=255)
                                                                       gcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTTCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAc                  <  1:4224470/93‑1 (MQ=255)
                                                                        cgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACg                 <  1:6083326/93‑1 (MQ=255)
                                                                         gTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGc                <  1:4578548/93‑1 (MQ=255)
                                                                         gTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGc                <  1:2287704/93‑1 (MQ=255)
                                                                         gTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCCGGGCTTCGCCTTGCAGATAACGATTAACATCGCGCAGCAACGc                >  2:5672718/1‑93 (MQ=255)
                                                                          tGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGcc               <  1:1187251/93‑1 (MQ=255)
                                                                          tGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGcc               >  1:2422788/1‑93 (MQ=255)
                                                                          tGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGcc               >  1:3044052/1‑93 (MQ=255)
                                                                           gACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCt              >  1:4657092/1‑93 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             <  2:785732/93‑1 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             >  1:5887557/1‑93 (MQ=255)
                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          <  2:4490145/93‑1 (MQ=255)
                                                                                gAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGc         >  2:2458728/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       <  1:4536661/93‑1 (MQ=255)
                                                                                   gCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCt      <  1:5444079/93‑1 (MQ=255)
                                                                                      aGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTc   >  2:3028474/1‑93 (MQ=255)
                                                                                       gcgcTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTcc  >  2:3236654/1‑93 (MQ=255)
                                                                                       gcgcTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTcc  <  2:839861/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  >  NC_000913/2953284‑2953469
                                                                                             |                                                                                            
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGC                                                                                              >  SRR4302533.3340677/1‑94 (MQ=60)
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGC                                                                                              >  SRR4302533.5140507/1‑94 (MQ=60)
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGC                                                                                              <  SRR4302533.5644311/94‑1 (MQ=60)
 GATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCT                                                                                             >  SRR4302533.4926714/1‑94 (MQ=60)
 GATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCT                                                                                             >  SRR4302533.4609074/1‑94 (MQ=60)
  ATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTT                                                                                            >  SRR4302533.4287176/1‑94 (MQ=60)
  ATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTT                                                                                            >  SRR4302533.4634650/1‑94 (MQ=60)
   TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTC                                                                                           >  SRR4302533.6300852/1‑94 (MQ=60)
    CAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCA                                                                                          <  SRR4302533.6104303/94‑1 (MQ=60)
    CAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCA                                                                                          <  SRR4302533.4610096/94‑1 (MQ=60)
     AGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCAT                                                                                         >  SRR4302533.4529829/1‑94 (MQ=60)
       CGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCA                                                                                       >  SRR4302533.2709907/1‑94 (MQ=60)
        GCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCAT                                                                                      >  SRR4302533.1241692/1‑94 (MQ=60)
         CATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATC                                                                                     >  SRR4302533.312911/1‑94 (MQ=60)
         CATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATC                                                                                     <  SRR4302533.5102939/94‑1 (MQ=60)
         CATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATC                                                                                     >  SRR4302533.2296128/1‑94 (MQ=60)
         CATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATC                                                                                     <  SRR4302533.4517030/94‑1 (MQ=60)
          CTCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCG                                                                                    <  SRR4302533.3573080/94‑1 (MQ=60)
          ATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCG                                                                                    >  SRR4302533.3607017/1‑94 (MQ=60)
          ATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCG                                                                                    >  SRR4302533.419065/1‑94 (MQ=60)
           TCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGG                                                                                   >  SRR4302533.4829472/1‑94 (MQ=60)
           TCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGG                                                                                   >  SRR4302533.1830058/1‑94 (MQ=60)
           TCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGG                                                                                   <  SRR4302533.2442059/94‑1 (MQ=60)
           TCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGG                                                                                   <  SRR4302533.5379443/94‑1 (MQ=60)
            CCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGG                                                                                  <  SRR4302533.3882356/94‑1 (MQ=60)
            CCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGG                                                                                  <  SRR4302533.4834972/94‑1 (MQ=60)
             CAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGC                                                                                 <  SRR4302533.2940245/94‑1 (MQ=60)
              AGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCG                                                                                >  SRR4302533.1182785/1‑94 (MQ=60)
               GTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGG                                                                               >  SRR4302533.2175275/1‑94 (MQ=60)
               GTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGG                                                                               >  SRR4302533.333658/1‑94 (MQ=60)
                TTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGC                                                                              >  SRR4302533.2455314/1‑94 (MQ=60)
                 TCACCCACTGCGTCGCGCCACTGCTGTTTTAACGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCG                                                                             >  SRR4302533.1898606/1‑94 (MQ=60)
                  CACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGG                                                                            <  SRR4302533.6299849/94‑1 (MQ=60)
                   ACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGT                                                                           <  SRR4302533.761768/94‑1 (MQ=60)
                   ACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGG                                                                           >  SRR4302533.1149848/1‑94 (MQ=60)
                    CCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTG                                                                          >  SRR4302533.3532682/1‑94 (MQ=60)
                    CCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGAGGTG                                                                          >  SRR4302533.2063398/1‑94 (MQ=60)
                       ACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTT                                                                       >  SRR4302533.2916652/1‑94 (MQ=60)
                       ACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTT                                                                       >  SRR4302533.5033927/1‑94 (MQ=60)
                        CTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTT                                                                      <  SRR4302533.1791517/94‑1 (MQ=60)
                        CTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTT                                                                      <  SRR4302533.428791/94‑1 (MQ=60)
                         TGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTG                                                                     >  SRR4302533.1222368/1‑94 (MQ=60)
                           CGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAT                                                                   >  SRR4302533.1528280/1‑94 (MQ=60)
                            GTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATA                                                                  <  SRR4302533.1503928/94‑1 (MQ=60)
                            GTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATA                                                                  <  SRR4302533.3458689/94‑1 (MQ=60)
                            GTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATA                                                                  >  SRR4302533.1685403/1‑94 (MQ=60)
                             TCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAA                                                                 <  SRR4302533.3152056/94‑1 (MQ=60)
                             TCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAA                                                                 <  SRR4302533.1754714/94‑1 (MQ=60)
                              CGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAC                                                                <  SRR4302533.2842197/94‑1 (MQ=60)
                              CGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAC                                                                >  SRR4302533.296402/1‑94 (MQ=60)
                              CGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAC                                                                >  SRR4302533.4586881/1‑94 (MQ=60)
                               GCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACC                                                               <  SRR4302533.2843426/94‑1 (MQ=60)
                               GCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACC                                                               <  SRR4302533.5225296/94‑1 (MQ=60)
                                CGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCG                                                              >  SRR4302533.5691373/1‑94 (MQ=60)
                                CGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCG                                                              >  SRR4302533.6026408/1‑94 (MQ=60)
                                 GCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGG                                                             >  SRR4302533.3450812/1‑94 (MQ=60)
                                 GCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGG                                                             >  SRR4302533.6065366/1‑94 (MQ=60)
                                  CCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGC                                                            <  SRR4302533.6298776/94‑1 (MQ=60)
                                  CCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGCGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGC                                                            >  SRR4302533.3405827/1‑94 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           <  SRR4302533.4458036/94‑1 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           >  SRR4302533.1105085/1‑94 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           >  SRR4302533.3114855/1‑94 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           >  SRR4302533.5073573/1‑94 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           <  SRR4302533.6296117/94‑1 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           >  SRR4302533.1584640/1‑94 (MQ=60)
                                    ACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGC                                                          <  SRR4302533.242545/94‑1 (MQ=60)
                                     CTGCTGTTTTACCGTATCAATACTCTCCATAATTTGCGCGTGACGGGAAGCCAGCGCTACATCATCGGGCGGCGGTGTTTTGATAAttttcgct                                                         >  SRR4302533.6338111/1‑86 (MQ=60)
                                     CTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCT                                                         >  SRR4302533.3297358/1‑94 (MQ=60)
                                      TGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTT                                                        >  SRR4302533.105067/1‑94 (MQ=60)
                                       GCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTC                                                       <  SRR4302533.1544486/94‑1 (MQ=60)
                                       GCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTC                                                       >  SRR4302533.3592883/1‑94 (MQ=60)
                                        CTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCG                                                      >  SRR4302533.5129108/1‑94 (MQ=60)
                                        CTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCG                                                      >  SRR4302533.2654413/1‑94 (MQ=60)
                                         TGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGC                                                     >  SRR4302533.24749/1‑94 (MQ=60)
                                          GTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCC                                                    >  SRR4302533.2077855/1‑94 (MQ=60)
                                            TTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTT                                                  <  SRR4302533.6172107/94‑1 (MQ=60)
                                              TACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGC                                                >  SRR4302533.1975377/1‑94 (MQ=60)
                                               ACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCA                                               >  SRR4302533.5707324/1‑94 (MQ=60)
                                               ACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCA                                               <  SRR4302533.4059887/94‑1 (MQ=60)
                                               ACCCTATCAATACGCGCCACAATCTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCA                                               <  SRR4302533.5155196/94‑1 (MQ=60)
                                                CCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAG                                              <  SRR4302533.3761504/94‑1 (MQ=60)
                                                CCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAG                                              <  SRR4302533.2222987/94‑1 (MQ=60)
                                                CCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAG                                              >  SRR4302533.280945/1‑94 (MQ=60)
                                                 CGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGA                                             >  SRR4302533.5612874/1‑94 (MQ=60)
                                                  GTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            <  SRR4302533.2016718/94‑1 (MQ=60)
                                                  GTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            >  SRR4302533.6050313/1‑94 (MQ=60)
                                                  GTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            <  SRR4302533.5540855/94‑1 (MQ=60)
                                                    ATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAA                                          >  SRR4302533.3782063/1‑94 (MQ=60)
                                                     TCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAAC                                         <  SRR4302533.5052922/94‑1 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        >  SRR4302533.1124712/1‑94 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        <  SRR4302533.4128996/94‑1 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        >  SRR4302533.4126563/1‑94 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        >  SRR4302533.4687616/1‑94 (MQ=60)
                                                        ATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAT                                      >  SRR4302533.886903/1‑94 (MQ=60)
                                                        ATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAT                                      <  SRR4302533.5727507/94‑1 (MQ=60)
                                                          ACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTA                                    >  SRR4302533.1482188/1‑94 (MQ=60)
                                                          ACGCGCCACAATTTGCGCGTGACGGGAAACCAGCGCTTCATCATCGGGCGGCTGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTA                                    <  SRR4302533.2656023/94‑1 (MQ=60)
                                                           CGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAA                                   >  SRR4302533.4289314/1‑94 (MQ=60)
                                                           CGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAA                                   >  SRR4302533.1317990/1‑94 (MQ=60)
                                                             CGCCGCAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATA                                 <  SRR4302533.1525207/94‑1 (MQ=60)
                                                             CGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATA                                 <  SRR4302533.5051445/94‑1 (MQ=60)
                                                             CGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATA                                 >  SRR4302533.3374850/1‑94 (MQ=60)
                                                              GCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATAT                                >  SRR4302533.4245646/1‑94 (MQ=60)
                                                              GCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATAT                                <  SRR4302533.3756296/94‑1 (MQ=60)
                                                               CCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATC                               >  SRR4302533.2221661/1‑94 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              <  SRR4302533.4214293/94‑1 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              >  SRR4302533.1721143/1‑94 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              >  SRR4302533.4401493/1‑94 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              <  SRR4302533.1474095/94‑1 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              <  SRR4302533.4737261/94‑1 (MQ=60)
                                                                 ATAATTGGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGC                             <  SRR4302533.5850615/94‑1 (MQ=60)
                                                                 ACAATTTGCGCGTGACGGGAAGCCATCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGC                             <  SRR4302533.6125168/94‑1 (MQ=60)
                                                                  CAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCG                            <  SRR4302533.5102923/94‑1 (MQ=60)
                                                                   AATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGC                           >  SRR4302533.642820/1‑94 (MQ=60)
                                                                   AATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGC                           >  SRR4302533.4096948/1‑94 (MQ=60)
                                                                    ATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCA                          >  SRR4302533.2988324/1‑94 (MQ=60)
                                                                     TTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAG                         <  SRR4302533.2019197/94‑1 (MQ=60)
                                                                     TTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAG                         <  SRR4302533.4934963/94‑1 (MQ=60)
                                                                     TTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAG                         <  SRR4302533.2844656/94‑1 (MQ=60)
                                                                       TGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATCACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCA                       <  SRR4302533.4470006/94‑1 (MQ=60)
                                                                       TGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCA                       >  SRR4302533.3303620/1‑94 (MQ=60)
                                                                        GCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAA                      >  SRR4302533.1455750/1‑94 (MQ=60)
                                                                        GCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAA                      >  SRR4302533.5711880/1‑94 (MQ=60)
                                                                         CGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAC                     <  SRR4302533.1279778/94‑1 (MQ=60)
                                                                         CGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAC                     <  SRR4302533.2624584/94‑1 (MQ=60)
                                                                         CGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAC                     <  SRR4302533.385035/94‑1 (MQ=60)
                                                                         CGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAC                     <  SRR4302533.3680853/94‑1 (MQ=60)
                                                                          GCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTTCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACG                    <  SRR4302533.4250010/94‑1 (MQ=60)
                                                                          GCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACG                    >  SRR4302533.3364699/1‑94 (MQ=60)
                                                                           CGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGC                   <  SRR4302533.6120109/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  <  SRR4302533.2301864/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  <  SRR4302533.4606297/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  >  SRR4302533.3062686/1‑94 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  <  SRR4302533.177232/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  >  SRR4302533.2437743/1‑94 (MQ=60)
                                                                             TGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCT                 <  SRR4302533.1194375/94‑1 (MQ=60)
                                                                             TGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCT                 >  SRR4302533.4685312/1‑94 (MQ=60)
                                                                              GACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTG                >  SRR4302533.5923129/1‑94 (MQ=60)
                                                                               ACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGC               >  SRR4302533.219455/1‑94 (MQ=60)
                                                                                CGGGAGGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCG              <  SRR4302533.4333601/94‑1 (MQ=60)
                                                                                CGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATACCCGGCGCTTCGCCTTGCAAATAACGATTAATATCGCGCCGCAACGCCTGCG              <  SRR4302533.5702870/94‑1 (MQ=60)
                                                                                  GGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGC            >  SRR4302533.4478891/1‑94 (MQ=60)
                                                                                  GGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGC            <  SRR4302533.1880081/94‑1 (MQ=60)
                                                                                   GAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCC           <  SRR4302533.1836361/94‑1 (MQ=60)
                                                                                     AGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCT         <  SRR4302533.291340/94‑1 (MQ=60)
                                                                                     AGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCT         <  SRR4302533.4564127/94‑1 (MQ=60)
                                                                                      GCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTT        <  SRR4302533.4445201/94‑1 (MQ=60)
                                                                                      GCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTT        <  SRR4302533.5476882/94‑1 (MQ=60)
                                                                                        CAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTC      >  SRR4302533.4536974/1‑94 (MQ=60)
                                                                                         AGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC     <  SRR4302533.3803983/94‑1 (MQ=60)
                                                                                          GCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCA    <  SRR4302533.1208428/94‑1 (MQ=60)
                                                                                           CGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAG   <  SRR4302533.4431905/94‑1 (MQ=60)
                                                                                            GCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  <  SRR4302533.4888381/94‑1 (MQ=60)
                                                                                            GCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  >  SRR4302533.466262/1‑94 (MQ=60)
                                                                                             |                                                                                            
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  >  NC_000913/2953284‑2953469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑