Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,965,001 T→C M486V (ATG→GTG)  ptsP ← PtsP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,965,0010TC100.0% 707.7 / NA 217M486V (ATG→GTG) ptsPPtsP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (108/109);  total (108/109)

Only 100 of 226 total aligned reads displayed.

CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT  >  NC_000913/2964911‑2965090
                                                                                          |                                                                                         
 ttCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAcccc                                                                                        <  1:2539510/93‑1 (MQ=255)
  tCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACcccc                                                                                       <  1:1504778/93‑1 (MQ=255)
   cGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCt                                                                                      <  2:20410/93‑1 (MQ=255)
     cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGa                                                                                    <  1:550633/93‑1 (MQ=255)
     cTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGa                                                                                    >  2:771766/1‑93 (MQ=255)
      tGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGGTTATCATTGAACATTTGCAGCACCCCCTGAt                                                                                   >  2:3446359/1‑93 (MQ=255)
       gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATa                                                                                  >  1:1661616/1‑93 (MQ=255)
       gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATa                                                                                  >  1:2517417/1‑93 (MQ=255)
        aTCGGCATGTAAGTCAGCTGCTTACTCGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATAc                                                                                 <  2:1202545/93‑1 (MQ=255)
          cGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTg                                                                               >  1:1604201/1‑93 (MQ=255)
           ggCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgc                                                                              <  1:1003197/93‑1 (MQ=255)
            gCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgcg                                                                             >  1:2335144/1‑93 (MQ=255)
            gCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgcg                                                                             <  2:5249532/93‑1 (MQ=255)
              aTGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGcc                                                                           <  1:2278496/93‑1 (MQ=255)
              aTGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGcc                                                                           >  1:795046/1‑93 (MQ=255)
               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  1:1792052/1‑93 (MQ=255)
               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  2:1763225/1‑93 (MQ=255)
               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  1:2856493/1‑93 (MQ=255)
                gTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCAc                                                                         <  2:4806459/93‑1 (MQ=255)
                  aaGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCt                                                                       <  1:5606237/93‑1 (MQ=255)
                      cAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttc                                                                   <  2:3592203/93‑1 (MQ=255)
                        gctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttctt                                                                 <  1:592901/93‑1 (MQ=255)
                        gctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttctt                                                                 >  1:3238297/1‑93 (MQ=255)
                        gctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttctt                                                                 <  1:412154/93‑1 (MQ=255)
                         ctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttc                                                                >  1:1632010/1‑93 (MQ=255)
                         ctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttc                                                                <  1:5784261/93‑1 (MQ=255)
                           gctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttctt                                                              >  1:2778515/1‑93 (MQ=255)
                            ctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttcttc                                                             >  2:2544052/1‑93 (MQ=255)
                              tATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCg                                                           >  2:4861859/1‑93 (MQ=255)
                              tATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCg                                                           <  2:653163/93‑1 (MQ=255)
                               aTCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttc                                                                <  2:4005035/87‑1 (MQ=255)
                               aTCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGa                                                          >  1:6005428/1‑93 (MQ=255)
                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                                                         <  1:6049472/93‑1 (MQ=255)
                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                                                         >  2:2301698/1‑93 (MQ=255)
                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                                                         >  1:2644359/1‑93 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                                                       <  1:4534991/93‑1 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                                                       <  1:478995/93‑1 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                                                       <  1:4345709/93‑1 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                                                       >  1:3998128/1‑93 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACgg                                                       >  1:5401127/1‑93 (MQ=255)
                                  tGCTCCGACATCCAGCGTACGCAAGGTGACGGCTTTATCATTGAACATTTGCAGCGCCCCCTGATACTGCTCCACCTGTTCTTCTTCCGACgg                                                       <  1:5738892/93‑1 (MQ=255)
                                   gCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACggg                                                      >  1:5867118/1‑93 (MQ=255)
                                    cTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGa                                                     >  1:4139919/1‑93 (MQ=255)
                                       cGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAc                                                  >  1:728371/1‑93 (MQ=255)
                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 >  2:5743821/1‑93 (MQ=255)
                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 >  1:3661078/1‑93 (MQ=255)
                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 >  1:246008/1‑93 (MQ=255)
                                          taTCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCCCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCAc                                               <  2:6103743/92‑1 (MQ=255)
                                           aTCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACt                                              <  2:2325318/93‑1 (MQ=255)
                                           aTCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACt                                              <  1:1213289/93‑1 (MQ=255)
                                            tCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                                             >  1:5548059/1‑93 (MQ=255)
                                            tCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                                             <  1:4036436/93‑1 (MQ=255)
                                             ccAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACttt                                            <  2:456973/93‑1 (MQ=255)
                                             acAGCGGACGCAAGGTGACGGTTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACttt                                            <  2:4168832/92‑1 (MQ=255)
                                              cAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTg                                           >  2:4048919/1‑93 (MQ=255)
                                               aGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgc                                          >  1:2125964/1‑93 (MQ=255)
                                                gCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgca                                         <  2:1120005/93‑1 (MQ=255)
                                                 cGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcag                                        <  1:624673/93‑1 (MQ=255)
                                                     cGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg                                    <  1:3755404/93‑1 (MQ=255)
                                                       cAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGaa                                  <  1:1549029/93‑1 (MQ=255)
                                                        aaGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                 <  1:4842362/93‑1 (MQ=255)
                                                          ggTGACGGGTTTATCATTGAGCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATgg                               <  1:5229751/93‑1 (MQ=255)
                                                          ggTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATgg                               >  2:2171028/1‑93 (MQ=255)
                                                           gTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATggg                              <  1:3083738/93‑1 (MQ=255)
                                                            tGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGa                             <  2:2403260/93‑1 (MQ=255)
                                                             gACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt                            >  2:4449248/1‑93 (MQ=255)
                                                             gACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt                            <  2:4939629/93‑1 (MQ=255)
                                                              aCGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAtt                           >  2:3298806/1‑93 (MQ=255)
                                                               cGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAttt                          <  2:5387121/93‑1 (MQ=255)
                                                                gggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc                         <  2:1052090/93‑1 (MQ=255)
                                                                 ggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCa                        <  1:312764/93‑1 (MQ=255)
                                                                 ggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCa                        <  1:5361904/93‑1 (MQ=255)
                                                                 ggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCa                        <  1:752312/93‑1 (MQ=255)
                                                                 ggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCa                        <  2:4487384/93‑1 (MQ=255)
                                                                  gTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAg                       >  1:3956314/1‑93 (MQ=255)
                                                                   tttATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGt                      <  2:2840615/93‑1 (MQ=255)
                                                                   tttATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGt                      <  2:5641266/93‑1 (MQ=255)
                                                                    ttATCATTGGACATTTGCAGCACCCCCTGATACTGCGCCACCAGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTg                     >  1:3385881/1‑93 (MQ=255)
                                                                    ttATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTg                     <  1:2942039/93‑1 (MQ=255)
                                                                     tATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGc                    >  1:3166294/1‑93 (MQ=255)
                                                                     tATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGc                    >  2:87455/1‑93 (MQ=255)
                                                                      aTCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCg                   <  1:2027147/93‑1 (MQ=255)
                                                                      aTCATTGAAAATTTGCAGCACCCCCTGATACCGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCg                   <  1:5097072/93‑1 (MQ=255)
                                                                       tCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa                  <  1:6156821/93‑1 (MQ=255)
                                                                       tCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa                  >  2:4548775/1‑93 (MQ=255)
                                                                        cATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCCGTGCGAt                 >  2:1418000/1‑93 (MQ=255)
                                                                         aTTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa                >  2:4235075/1‑93 (MQ=255)
                                                                         aTTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa                <  2:4620291/93‑1 (MQ=255)
                                                                         aTTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa                <  1:5170789/93‑1 (MQ=255)
                                                                          ttGAACATTTGCAGCTCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaa               <  2:271710/93‑1 (MQ=255)
                                                                            gAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAg             <  2:4905708/93‑1 (MQ=255)
                                                                            gAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAg             <  2:2589576/93‑1 (MQ=255)
                                                                            gAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAg             >  2:519687/1‑93 (MQ=255)
                                                                              aCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCGCTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAc           <  1:5522842/93‑1 (MQ=255)
                                                                              aCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAc           >  1:2316205/1‑93 (MQ=255)
                                                                              aCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAc           <  1:4999925/93‑1 (MQ=255)
                                                                               cATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAcc          <  2:3504423/93‑1 (MQ=255)
                                                                                aTTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCg         >  2:5921098/1‑93 (MQ=255)
                                                                                aTTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCg         >  1:3544480/1‑93 (MQ=255)
                                                                                   tGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATg      <  2:3048398/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT  >  NC_000913/2964911‑2965090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  NC_000913/2964908‑2965094
                                                                                             |                                                                                             
TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAC                                                                                               <  SRR4302533.2120026/94‑1 (MQ=60)
 CTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACC                                                                                              >  SRR4302533.5486082/1‑94 (MQ=60)
 CTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACC                                                                                              >  SRR4302533.3919141/1‑94 (MQ=60)
   CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGTACATTTGCAGCACCCC                                                                                            >  SRR4302533.1615308/1‑94 (MQ=60)
    TTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCC                                                                                           <  SRR4302533.2555142/94‑1 (MQ=60)
     TCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCT                                                                                          <  SRR4302533.2431058/94‑1 (MQ=60)
     TCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCT                                                                                          <  SRR4302533.1513853/94‑1 (MQ=60)
      CGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTG                                                                                         >  SRR4302533.977381/1‑94 (MQ=60)
        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       >  SRR4302533.3386779/1‑94 (MQ=60)
        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       <  SRR4302533.5495391/94‑1 (MQ=60)
        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       <  SRR4302533.553898/94‑1 (MQ=60)
        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       <  SRR4302533.754123/94‑1 (MQ=60)
        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       <  SRR4302533.2056054/94‑1 (MQ=60)
         TGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATA                                                                                      <  SRR4302533.1586909/94‑1 (MQ=60)
         TGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATA                                                                                      >  SRR4302533.2532930/1‑94 (MQ=60)
         TGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATA                                                                                      >  SRR4302533.1671662/1‑94 (MQ=60)
         TGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATA                                                                                      >  SRR4302533.6233684/1‑94 (MQ=60)
            TCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTG                                                                                   >  SRR4302533.1613914/1‑94 (MQ=60)
            TCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTG                                                                                   <  SRR4302533.5309101/94‑1 (MQ=60)
             CGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGC                                                                                  <  SRR4302533.4918197/94‑1 (MQ=60)
              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCG                                                                                 >  SRR4302533.2227616/1‑94 (MQ=60)
              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCG                                                                                 >  SRR4302533.2349599/1‑94 (MQ=60)
              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCG                                                                                 <  SRR4302533.1009060/94‑1 (MQ=60)
                CATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCC                                                                               >  SRR4302533.799724/1‑94 (MQ=60)
                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302533.2874068/1‑94 (MQ=60)
                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302533.2874069/1‑94 (MQ=60)
                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              <  SRR4302533.2292605/94‑1 (MQ=60)
                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302533.1802951/1‑94 (MQ=60)
                     AAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTG                                                                          <  SRR4302533.5640113/94‑1 (MQ=60)
                     AAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTG                                                                          >  SRR4302533.4003656/1‑94 (MQ=60)
                      AGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGT                                                                         >  SRR4302533.787328/1‑94 (MQ=60)
                         CAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCT                                                                      <  SRR4302533.2885520/94‑1 (MQ=60)
                          AGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTT                                                                     <  SRR4302533.1814257/94‑1 (MQ=60)
                          AGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTT                                                                     >  SRR4302533.3258253/1‑94 (MQ=60)
                           GCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGACCATTTGCAGAACCCCCTGATACTGCGCCACCTGTTCTTT                                                                    >  SRR4302533.1810220/1‑94 (MQ=60)
                           GCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTC                                                                    <  SRR4302533.596401/94‑1 (MQ=60)
                           GCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTC                                                                    >  SRR4302533.1641895/1‑94 (MQ=60)
                           GCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTC                                                                    <  SRR4302533.414628/94‑1 (MQ=60)
                            CTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCT                                                                   <  SRR4302533.5819193/94‑1 (MQ=60)
                            CTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCT                                                                   <  SRR4302533.6106536/94‑1 (MQ=60)
                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302533.5797953/1‑94 (MQ=60)
                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302533.2795581/1‑94 (MQ=60)
                               CTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCC                                                                >  SRR4302533.2498674/1‑94 (MQ=60)
                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               <  SRR4302533.3164650/94‑1 (MQ=60)
                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               >  SRR4302533.4430309/1‑94 (MQ=60)
                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               >  SRR4302533.5961721/1‑94 (MQ=60)
                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGGTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               >  SRR4302533.2572194/1‑94 (MQ=60)
                                 TATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATTATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGA                                                              <  SRR4302533.5553421/94‑1 (MQ=60)
                                 TATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTataca                                                              >  SRR4302533.4029414/1‑89 (MQ=60)
                                 TATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGA                                                              >  SRR4302533.6041816/1‑94 (MQ=60)
                                  ATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAC                                                             >  SRR4302533.2660607/1‑94 (MQ=60)
                                   TCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACG                                                            >  SRR4302533.517212/1‑94 (MQ=60)
                                   TCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACG                                                            <  SRR4302533.6086122/94‑1 (MQ=60)
                                    CTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGG                                                           >  SRR4302533.5433584/1‑94 (MQ=60)
                                    CTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGG                                                           >  SRR4302533.4022470/1‑94 (MQ=60)
                                    CTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGG                                                           >  SRR4302533.3079196/1‑94 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          >  SRR4302533.5902582/1‑94 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.481857/94‑1 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.4562451/94‑1 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.2558355/94‑1 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.4371900/94‑1 (MQ=60)
                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGCTTTATCATTGAACATTTGCAGCGCCCCCTGATACTGCTCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.5773526/94‑1 (MQ=60)
                                     TGCTCCGACATCCAGAGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302533.5572324/94‑1 (MQ=60)
                                      GCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGA                                                         >  SRR4302533.4165029/1‑94 (MQ=60)
                                         CCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAC                                                      >  SRR4302533.732674/1‑94 (MQ=60)
                                          CGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACC                                                     >  SRR4302533.247531/1‑94 (MQ=60)
                                          CGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACC                                                     >  SRR4302533.3683589/1‑94 (MQ=60)
                                          CGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACC                                                     <  SRR4302533.3920288/94‑1 (MQ=60)
                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 <  SRR4302533.1220582/94‑1 (MQ=60)
                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302533.5581564/1‑94 (MQ=60)
                                               TCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTT                                                <  SRR4302533.4061003/94‑1 (MQ=60)
                                               TCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTT                                                >  SRR4302533.3592326/1‑94 (MQ=60)
                                               TCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTT                                                <  SRR4302533.903979/94‑1 (MQ=60)
                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               >  SRR4302533.5421037/1‑94 (MQ=60)
                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               >  SRR4302533.2349419/1‑94 (MQ=60)
                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               <  SRR4302533.3766337/94‑1 (MQ=60)
                                                CCAGCGTACGCAAGGCGACGGGTTTATCATTGAACATTTACAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               >  SRR4302533.2617278/1‑94 (MQ=60)
                                                 CAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGC                                              >  SRR4302533.1344287/1‑94 (MQ=60)
                                                 CAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGC                                              >  SRR4302533.5717489/1‑94 (MQ=60)
                                                 CAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGC                                              >  SRR4302533.2139070/1‑94 (MQ=60)
                                                 CAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGA                                              >  SRR4302533.5102818/1‑94 (MQ=60)
                                                    CGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGC                                           <  SRR4302533.628341/94‑1 (MQ=60)
                                                     GTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          >  SRR4302533.4147561/1‑94 (MQ=60)
                                                     GTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          >  SRR4302533.4192314/1‑94 (MQ=60)
                                                       ACGCGAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATG                                        <  SRR4302533.5951643/94‑1 (MQ=60)
                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       >  SRR4302533.3186639/1‑94 (MQ=60)
                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       <  SRR4302533.3778420/94‑1 (MQ=60)
                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       >  SRR4302533.2730303/1‑94 (MQ=60)
                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     <  SRR4302533.1558367/94‑1 (MQ=60)
                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     >  SRR4302533.6074422/1‑94 (MQ=60)
                                                           AAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATG                                    <  SRR4302533.1677691/94‑1 (MQ=60)
                                                           AAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATG                                    <  SRR4302533.4871710/94‑1 (MQ=60)
                                                            AGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGG                                   <  SRR4302533.2319357/94‑1 (MQ=60)
                                                            AGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGG                                   >  SRR4302533.5469587/1‑94 (MQ=60)
                                                             GGTGACGGGTTTATCATTGAGCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGG                                  <  SRR4302533.5261209/94‑1 (MQ=60)
                                                              GTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGA                                 <  SRR4302533.3038236/94‑1 (MQ=60)
                                                              GTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGA                                 <  SRR4302533.3102695/94‑1 (MQ=60)
                                                                 ACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTT                              >  SRR4302533.4694450/1‑94 (MQ=60)
                                                                   GGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCA                            <  SRR4302533.4730471/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           <  SRR4302533.5394108/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           <  SRR4302533.152197/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           <  SRR4302533.314674/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           <  SRR4302533.4859576/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           <  SRR4302533.756746/94‑1 (MQ=60)
                                                                    GGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAG                           >  SRR4302533.3980430/1‑94 (MQ=60)
                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          >  SRR4302533.5235731/1‑94 (MQ=60)
                                                                      TTTATCATTGGACATTTGCAGCACCCCCTGATACTGCGCCACCAGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTG                         >  SRR4302533.3406787/1‑94 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTGTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302533.4110980/1‑94 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302533.5718391/1‑94 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302533.599734/1‑94 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        <  SRR4302533.2960005/94‑1 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302533.3742773/1‑94 (MQ=60)
                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302533.3185776/1‑94 (MQ=60)
                                                                         ATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGA                      <  SRR4302533.2039662/94‑1 (MQ=60)
                                                                         ATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGA                      >  SRR4302533.5848236/1‑94 (MQ=60)
                                                                         ATCATTGAAAATTTGCAGCACCCCCTGATACCGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGA                      <  SRR4302533.5127866/94‑1 (MQ=60)
                                                                          TCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAT                     <  SRR4302533.1604021/94‑1 (MQ=60)
                                                                          TCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAT                     <  SRR4302533.6193859/94‑1 (MQ=60)
                                                                            ATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAA                   <  SRR4302533.5201995/94‑1 (MQ=60)
                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 >  SRR4302533.5599834/1‑94 (MQ=60)
                                                                                AACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               <  SRR4302533.1178260/94‑1 (MQ=60)
                                                                                AACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               >  SRR4302533.2330546/1‑94 (MQ=60)
                                                                                AACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               <  SRR4302533.6229513/94‑1 (MQ=60)
                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCGCTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302533.5556187/94‑1 (MQ=60)
                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302533.5131109/94‑1 (MQ=60)
                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302533.5030234/94‑1 (MQ=60)
                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302533.1643519/94‑1 (MQ=60)
                                                                                  CATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCG             <  SRR4302533.2341314/94‑1 (MQ=60)
                                                                                  CATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCG             >  SRR4302533.3566327/1‑94 (MQ=60)
                                                                                   ATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGA            >  SRR4302533.3347398/1‑94 (MQ=60)
                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302533.2546738/94‑1 (MQ=60)
                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302533.3498059/94‑1 (MQ=60)
                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302533.4094459/94‑1 (MQ=60)
                                                                                        CAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCA       <  SRR4302533.658633/94‑1 (MQ=60)
                                                                                         AGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT      >  SRR4302533.3125468/1‑94 (MQ=60)
                                                                                          GCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATC     >  SRR4302533.1831945/1‑94 (MQ=60)
                                                                                          GCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATC     >  SRR4302533.2325150/1‑94 (MQ=60)
                                                                                           CACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCA    <  SRR4302533.4646074/94‑1 (MQ=60)
                                                                                           CACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCA    >  SRR4302533.4127953/1‑94 (MQ=60)
                                                                                           CACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCA    >  SRR4302533.2392022/1‑94 (MQ=60)
                                                                                           CACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCA    <  SRR4302533.2245723/94‑1 (MQ=60)
                                                                                            ACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAA   >  SRR4302533.6065297/1‑94 (MQ=60)
                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302533.4830314/1‑94 (MQ=60)
                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  <  SRR4302533.4114037/94‑1 (MQ=60)
                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  <  SRR4302533.2502630/94‑1 (MQ=60)
                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302533.909304/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  NC_000913/2964908‑2965094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑