Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,807	A→G	L372P (CTG→CCG)	lysA ←	diaminopimelate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,807	0	A	G	100.0%	838.1 / NA	257	L372P (CTG→CCG)	lysA	diaminopimelate decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (126/131); total (126/131)

Only 100 of 259 total aligned reads displayed.

GCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCA  >  NC_000913/2975717‑2975896

gcCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGt                                                                                         <  1:6247754/93‑1 (MQ=255)

 ccTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTa                                                                                        >  2:2497392/1‑93 (MQ=255)

  cTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTAc                                                                                       <  1:1035785/93‑1 (MQ=255)

  cTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTAc                                                                                       <  1:3905990/93‑1 (MQ=255)

  cTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTAc                                                                                       <  2:2981462/93‑1 (MQ=255)

   tGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTAcc                                                                                      >  2:4350828/1‑93 (MQ=255)

    gACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCa                                                                                     >  1:5271702/1‑93 (MQ=255)

    gACCATTATCAAACAGAACTTCTGGGAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGGGCCATATGCCCCTGTATCATGCGGTACCa                                                                                     >  2:2681636/1‑93 (MQ=255)

      ccATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGa                                                                                   >  2:4078462/1‑93 (MQ=255)

      ccATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGa                                                                                   <  1:463240/93‑1 (MQ=255)

      acATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGa                                                                                   <  1:397844/92‑1 (MQ=255)

       cATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGAt                                                                                  >  2:4195695/1‑93 (MQ=255)

       cATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGAt                                                                                  <  2:5962577/93‑1 (MQ=255)

        aTTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATa                                                                                 >  2:4500219/1‑93 (MQ=255)

        aTTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATa                                                                                 >  1:4292858/1‑93 (MQ=255)

        aTTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATa                                                                                 >  1:4984963/1‑93 (MQ=255)

         ttATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATaa                                                                                >  2:1322400/1‑93 (MQ=255)

         ttATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATaa                                                                                <  1:3464782/93‑1 (MQ=255)

           aTCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAAta                                                                              >  1:2033889/1‑92 (MQ=255)

            tCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCa                                                                             >  2:5275539/1‑93 (MQ=255)

              aaaCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCAcc                                                                           <  1:3112876/93‑1 (MQ=255)

               aaCAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCt                                                                          <  2:3902899/93‑1 (MQ=255)

                 cAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGc                                                                        >  1:1018548/1‑93 (MQ=255)

                    aaCTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCtt                                                                     <  2:1069840/93‑1 (MQ=255)

                     aCTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTc                                                                    <  1:546075/93‑1 (MQ=255)

                     aCTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTc                                                                    <  2:4618327/93‑1 (MQ=255)

                       ttCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCAc                                                                  >  2:5881698/1‑93 (MQ=255)

                        tCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACt                                                                 <  1:923590/93‑1 (MQ=255)

                        tCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACt                                                                 >  2:3426440/1‑93 (MQ=255)

                         cTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACtt                                                                <  2:1202473/93‑1 (MQ=255)

                          tGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTc                                                               >  2:3013703/1‑93 (MQ=255)

                          tGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTc                                                               >  1:8302/1‑93 (MQ=255)

                          tGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTc                                                               <  1:5994534/93‑1 (MQ=255)

                          tGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTc                                                               >  1:2420106/1‑93 (MQ=255)

                          tGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTc                                                               <  1:3827650/93‑1 (MQ=255)

                           ggTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTcc                                                              >  2:4860733/1‑93 (MQ=255)

                           ggTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTcc                                                              <  1:5507668/93‑1 (MQ=255)

                              aaCAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGc                                                           >  1:1836282/1‑93 (MQ=255)

                               aCAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCa                                                          <  1:1338475/93‑1 (MQ=255)

                                cAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCaa                                                         <  1:1659740/93‑1 (MQ=255)

                                cAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCaa                                                         <  1:2235722/93‑1 (MQ=255)

                                cAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCaa                                                         <  2:2107693/93‑1 (MQ=255)

                                  gCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAgg                                                       >  1:4379568/1‑93 (MQ=255)

                                  gCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAgg                                                       <  2:5935819/93‑1 (MQ=255)

                                    ggACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGgcg                                                     >  2:3276256/1‑93 (MQ=255)

                                    ggACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGgcg                                                     <  2:1365858/93‑1 (MQ=255)

                                     gACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGgcgc                                                    <  1:4523700/93‑1 (MQ=255)

                                     gACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGgcgc                                                    >  1:3313354/1‑93 (MQ=255)

                                      aCGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGcgcg                                                   >  2:2552613/1‑93 (MQ=255)

                                      aCGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGcgcg                                                   >  1:2947289/1‑93 (MQ=255)

                                       cGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCgg                                                  <  2:2223652/93‑1 (MQ=255)

                                         gCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGt                                                >  2:3393899/1‑93 (MQ=255)

                                           tATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGttt                                              <  2:2839849/93‑1 (MQ=255)

                                           tATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGttt                                              <  1:5815862/93‑1 (MQ=255)

                                           tATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGttt                                              >  1:4714974/1‑93 (MQ=255)

                                            aTTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTc                                             >  2:6282966/1‑93 (MQ=255)

                                             ttGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATAACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCa                                            >  2:4447949/1‑93 (MQ=255)

                                               gTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAAc                                          >  2:703200/1‑93 (MQ=255)

                                                tAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACa                                         <  1:4794588/93‑1 (MQ=255)

                                                tAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACa                                         <  2:3299850/93‑1 (MQ=255)

                                                   ttGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACAttt                                      >  1:372754/1‑93 (MQ=255)

                                                   ttGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACAttt                                      >  1:1939379/1‑93 (MQ=255)

                                                      gATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTccc                                   <  1:233145/93‑1 (MQ=255)

                                                         gACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCt                                <  1:6157436/93‑1 (MQ=255)

                                                             ttGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCct                            >  1:2134129/1‑93 (MQ=255)

                                                             ttGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCct                            <  2:3865534/93‑1 (MQ=255)

                                                              tGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctg                           <  1:4237940/93‑1 (MQ=255)

                                                                aTGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCctgct                         <  2:1410146/93‑1 (MQ=255)

                                                                  gcgcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTgg                       <  2:6173770/93‑1 (MQ=255)

                                                                  gcgcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTgg                       <  1:2879438/93‑1 (MQ=255)

                                                                   cgcCATATGCCCCTGTATCATGCGGTACCAGATAATCGCCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTggg                      <  2:5578652/93‑1 (MQ=255)

                                                                   cgcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTggg                      <  2:3728725/93‑1 (MQ=255)

                                                                    gcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGt                     >  2:2607679/1‑93 (MQ=255)

                                                                    gcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGt                     >  1:3777488/1‑93 (MQ=255)

                                                                    gcCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGt                     <  2:4327359/93‑1 (MQ=255)

                                                                     ccATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTa                    >  2:5638733/1‑93 (MQ=255)

                                                                     ccATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGGTTCAACATTTCCCCCTTCCTGCTGGGTa                    >  1:3415706/1‑93 (MQ=255)

                                                                       atatGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTaaa                  <  1:5687836/93‑1 (MQ=255)

                                                                       atatGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTaaa                  >  2:1782889/1‑93 (MQ=255)

                                                                        tatGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCTCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAg                 >  1:3621860/1‑93 (MQ=255)

                                                                        tatGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAg                 >  1:1312838/1‑93 (MQ=255)

                                                                         atGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGa                <  2:4556926/93‑1 (MQ=255)

                                                                         atGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGa                <  1:4317138/93‑1 (MQ=255)

                                                                          tGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGAc               <  2:180073/93‑1 (MQ=255)

                                                                          tGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGAc               <  1:2089510/93‑1 (MQ=255)

                                                                           gCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACa              <  1:6020010/93‑1 (MQ=255)

                                                                            ccccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACAt             <  2:2246142/93‑1 (MQ=255)

                                                                            ccccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACAt             <  1:74432/93‑1 (MQ=255)

                                                                            ccccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACAt             <  1:1129458/93‑1 (MQ=255)

                                                                             cccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATc            >  2:1753863/1‑93 (MQ=255)

                                                                             cccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATc            <  2:540224/93‑1 (MQ=255)

                                                                             cccTATATGATGCGGTACCAGATAATCACCTGCCTTCACTTACGGCAAGGCGCGGGTTTCAACATTACCCCCTTCCTGCTGGGTAAAGACATc            <  2:760349/93‑1 (MQ=255)

                                                                              ccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCg           <  2:1555398/93‑1 (MQ=255)

                                                                              ccTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCg           <  1:5109169/93‑1 (MQ=255)

                                                                               cTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGc          >  2:860948/1‑93 (MQ=255)

                                                                                tGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGcc         <  1:215533/93‑1 (MQ=255)

                                                                                 gTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGccc        >  1:1549290/1‑93 (MQ=255)

                                                                                   aTCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGa      <  2:4275174/93‑1 (MQ=255)

                                                                                     cATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAtt    >  1:2689821/1‑93 (MQ=255)

                                                                                      aTGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTc   >  2:5055507/1‑93 (MQ=255)

GCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCA  >  NC_000913/2975717‑2975896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATA  >  NC_000913/2975715‑2975900

GCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGG                                                                                              >  SRR4302533.1162413/1‑94 (MQ=60)

 CGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGT                                                                                             <  SRR4302533.3836214/94‑1 (MQ=60)

  GCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTA                                                                                            <  SRR4302533.6285141/94‑1 (MQ=60)

    CTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACC                                                                                          >  SRR4302533.2139529/1‑94 (MQ=60)

    CTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACC                                                                                          <  SRR4302533.1041874/94‑1 (MQ=60)

    CTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACC                                                                                          <  SRR4302533.3929848/94‑1 (MQ=60)

     TGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCA                                                                                         >  SRR4302533.5303317/1‑94 (MQ=60)

      GACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAG                                                                                        <  SRR4302533.214611/94‑1 (MQ=60)

       ACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGA                                                                                       <  SRR4302533.1342137/94‑1 (MQ=60)

        CCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGAT                                                                                      <  SRR4302533.1956568/94‑1 (MQ=60)

        CCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGAT                                                                                      <  SRR4302533.466002/94‑1 (MQ=60)

        ACATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGAT                                                                                      <  SRR4302533.400249/94‑1 (MQ=60)

         CATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATA                                                                                     >  SRR4302533.5015188/1‑94 (MQ=60)

         CATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATA                                                                                     >  SRR4302533.4318674/1‑94 (MQ=60)

         CATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATA                                                                                     <  SRR4302533.867428/94‑1 (MQ=60)

           TTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAAT                                                                                   >  SRR4302533.6031579/1‑94 (MQ=60)

           TTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAAT                                                                                   <  SRR4302533.3486168/94‑1 (MQ=60)

            TATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATC                                                                                  >  SRR4302533.6043265/1‑94 (MQ=60)

            TATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATA                                                                                  >  SRR4302533.2046435/1‑94 (MQ=60)

            TATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGGAGTTGGATGACATTGATGCGCCATATGCCGCTGTATCATGCGGTACCAGATAATA                                                                                  >  SRR4302533.2365419/1‑94 (MQ=60)

             ATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCA                                                                                 <  SRR4302533.5038413/94‑1 (MQ=60)

              TCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCAC                                                                                <  SRR4302533.4676905/94‑1 (MQ=60)

               CAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACC                                                                               <  SRR4302533.4217053/94‑1 (MQ=60)

               CAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAGGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACC                                                                               >  SRR4302533.3413874/1‑94 (MQ=60)

                AAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCT                                                                              <  SRR4302533.3132020/94‑1 (MQ=60)

                AAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCT                                                                              >  SRR4302533.3105527/1‑94 (MQ=60)

                AAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCT                                                                              >  SRR4302533.5258671/1‑94 (MQ=60)

                 AACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTG                                                                             >  SRR4302533.5495298/1‑94 (MQ=60)

                 AACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTG                                                                             >  SRR4302533.2169454/1‑94 (MQ=60)

                  ACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGC                                                                            >  SRR4302533.2165751/1‑94 (MQ=60)

                  ACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGC                                                                            >  SRR4302533.1024537/1‑94 (MQ=60)

                    AGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCT                                                                          <  SRR4302533.2727010/94‑1 (MQ=60)

                       ACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCA                                                                       <  SRR4302533.549321/94‑1 (MQ=60)

                         TTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACT                                                                     >  SRR4302533.4941082/1‑94 (MQ=60)

                         TTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACT                                                                     <  SRR4302533.1564891/94‑1 (MQ=60)

                          TCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTT                                                                    <  SRR4302533.929015/94‑1 (MQ=60)

                           CTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTC                                                                   >  SRR4302533.1867187/1‑94 (MQ=60)

                           CTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTC                                                                   >  SRR4302533.2435053/1‑94 (MQ=60)

                           CTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTC                                                                   >  SRR4302533.8376/1‑94 (MQ=60)

                            TGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCC                                                                  <  SRR4302533.3761013/94‑1 (MQ=60)

                            TGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCC                                                                  <  SRR4302533.3851050/94‑1 (MQ=60)

                            TGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCC                                                                  <  SRR4302533.3437439/94‑1 (MQ=60)

                            TGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCC                                                                  <  SRR4302533.6030841/94‑1 (MQ=60)

                             GGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCG                                                                 <  SRR4302533.5540908/94‑1 (MQ=60)

                             GGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCG                                                                 <  SRR4302533.3748449/94‑1 (MQ=60)

                               TAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGC                                                               >  SRR4302533.1847483/1‑94 (MQ=60)

                                AACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCA                                                              >  SRR4302533.383434/1‑94 (MQ=60)

                                AACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCA                                                              <  SRR4302533.5745489/94‑1 (MQ=60)

                                 ACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAA                                                             <  SRR4302533.1346529/94‑1 (MQ=60)

                                  acggcggcgggttatggtattggaatgcaatgattccgcaataggccccGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.3726819/45‑1 (MQ=60)

                                  CAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.1669777/94‑1 (MQ=60)

                                  CAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.5087374/94‑1 (MQ=60)

                                  CAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.2249544/94‑1 (MQ=60)

                                  CAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.2266620/94‑1 (MQ=60)

                                  CAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAG                                                            <  SRR4302533.4646661/94‑1 (MQ=60)

                                   AGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGG                                                           >  SRR4302533.3862716/1‑94 (MQ=60)

                                   AGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGG                                                           >  SRR4302533.4406009/1‑94 (MQ=60)

                                   AGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGG                                                           <  SRR4302533.4491649/94‑1 (MQ=60)

                                    GCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGC                                                          <  SRR4302533.3564570/94‑1 (MQ=60)

                                      GGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGC                                                        >  SRR4302533.3333810/1‑94 (MQ=60)

                                       GACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCG                                                       >  SRR4302533.2965315/1‑94 (MQ=60)

                                       GACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCG                                                       <  SRR4302533.1066068/94‑1 (MQ=60)

                                       GACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCG                                                       >  SRR4302533.4274096/1‑94 (MQ=60)

                                       GACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCG                                                       <  SRR4302533.4551083/94‑1 (MQ=60)

                                        ACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGG                                                      >  SRR4302533.1969931/1‑94 (MQ=60)

                                         CGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGG                                                     <  SRR4302533.259370/94‑1 (MQ=60)

                                          GGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGT                                                    >  SRR4302533.771029/1‑94 (MQ=60)

                                          GGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGT                                                    <  SRR4302533.1029519/94‑1 (MQ=60)

                                          GGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCACGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGT                                                    <  SRR4302533.3995803/94‑1 (MQ=60)

                                            CTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTT                                                  >  SRR4302533.4743549/1‑94 (MQ=60)

                                            CTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTT                                                  >  SRR4302533.6181588/1‑94 (MQ=60)

                                             TATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTC                                                 >  SRR4302533.2097186/1‑94 (MQ=60)

                                             TATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTC                                                 >  SRR4302533.2819963/1‑94 (MQ=60)

                                             TATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTC                                                 <  SRR4302533.5850988/94‑1 (MQ=60)

                                              ATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCA                                                <  SRR4302533.747542/94‑1 (MQ=60)

                                               TTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAA                                               <  SRR4302533.3094919/94‑1 (MQ=60)

                                                TGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAAC                                              >  SRR4302533.4380965/1‑94 (MQ=60)

                                                  TAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACAT                                            <  SRR4302533.4823632/94‑1 (MQ=60)

                                                   AGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATT                                           >  SRR4302533.2302294/1‑94 (MQ=60)

                                                   AGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATT                                           >  SRR4302533.6192769/1‑94 (MQ=60)

                                                    GTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTT                                          >  SRR4302533.375005/1‑94 (MQ=60)

                                                    GTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTT                                          >  SRR4302533.1951207/1‑94 (MQ=60)

                                                     TTGGATGAGATTGATGCGCCATACGCCCCTGTATCATGCGGTCCCAGATAACCCCCTGCCTTCCCTTCCGGCAAGGCGCGGGTTTCAACATTTC                                         <  SRR4302533.5619683/94‑1 (MQ=60)

                                                     TTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTC                                         >  SRR4302533.1098349/1‑94 (MQ=60)

                                                       GGATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCC                                       >  SRR4302533.3809969/1‑94 (MQ=60)

                                                        GATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCC                                      <  SRR4302533.234585/94‑1 (MQ=60)

                                                        GATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCC                                      >  SRR4302533.3687402/1‑94 (MQ=60)

                                                         ATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCC                                     >  SRR4302533.4319526/1‑94 (MQ=60)

                                                         ATGACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCC                                     <  SRR4302533.6242069/94‑1 (MQ=60)

                                                           GACATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTT                                   <  SRR4302533.6194476/94‑1 (MQ=60)

                                                              ATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCT                                >  SRR4302533.2147289/1‑94 (MQ=60)

                                                              ATTGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCT                                <  SRR4302533.5411760/94‑1 (MQ=60)

                                                                TGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGC                              >  SRR4302533.5883493/1‑94 (MQ=60)

                                                                TGATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGC                              <  SRR4302533.4263538/94‑1 (MQ=60)

                                                                 GATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCT                             <  SRR4302533.1404122/94‑1 (MQ=60)

                                                                 GATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCT                             <  SRR4302533.2447777/94‑1 (MQ=60)

                                                                  ATGCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGCTTCAACATTTCCCCCTTCCTGCTG                            >  SRR4302533.357897/1‑94 (MQ=60)

                                                                    GCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGG                          <  SRR4302533.2897155/94‑1 (MQ=60)

                                                                    GCGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGG                          <  SRR4302533.1562804/94‑1 (MQ=60)

                                                                     CGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGT                         >  SRR4302533.3800635/1‑94 (MQ=60)

                                                                     CGCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGT                         >  SRR4302533.3950487/1‑94 (MQ=60)

                                                                      GCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTA                        <  SRR4302533.5242547/94‑1 (MQ=60)

                                                                      GCCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGGTTCAACATTTCCCCCTTCCTGCTGGGTA                        >  SRR4302533.3436811/1‑94 (MQ=60)

                                                                       CCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAA                       >  SRR4302533.925937/1‑94 (MQ=60)

                                                                       CCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAA                       >  SRR4302533.3263170/1‑94 (MQ=60)

                                                                       CCATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAA                       <  SRR4302533.4483914/94‑1 (MQ=60)

                                                                        CATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAA                      <  SRR4302533.4598957/94‑1 (MQ=60)

                                                                         ATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCTCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAG                     >  SRR4302533.3644146/1‑94 (MQ=60)

                                                                         ATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAG                     >  SRR4302533.1320716/1‑94 (MQ=60)

                                                                         ATATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAG                     <  SRR4302533.5722190/94‑1 (MQ=60)

                                                                          TATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGA                    >  SRR4302533.1184782/1‑94 (MQ=60)

                                                                           ATGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGAC                   <  SRR4302533.4343135/94‑1 (MQ=60)

                                                                            TGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACA                  <  SRR4302533.2102376/94‑1 (MQ=60)

                                                                            TGCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACA                  <  SRR4302533.5521632/94‑1 (MQ=60)

                                                                             GCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACAT                 >  SRR4302533.434043/1‑94 (MQ=60)

                                                                             GCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACAT                 <  SRR4302533.6056487/94‑1 (MQ=60)

                                                                             GCCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCATCCTGCTGGGTAAAGACAT                 >  SRR4302533.173267/1‑94 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                <  SRR4302533.5847076/94‑1 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                <  SRR4302533.1136170/94‑1 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                >  SRR4302533.4809032/1‑94 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                >  SRR4302533.2927989/1‑94 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                <  SRR4302533.4767967/94‑1 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                <  SRR4302533.1338074/94‑1 (MQ=60)

                                                                              CCCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATC                <  SRR4302533.74916/94‑1 (MQ=60)

                                                                               CCCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCC               >  SRR4302533.3395880/1‑94 (MQ=60)

                                                                                CCTGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGC              <  SRR4302533.5140042/94‑1 (MQ=60)

                                                                                  TGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCC            <  SRR4302533.216889/94‑1 (MQ=60)

                                                                                  TGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCC            <  SRR4302533.5636221/94‑1 (MQ=60)

                                                                                  TGTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCC            >  SRR4302533.1558628/1‑94 (MQ=60)

                                                                                   GTATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCTCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTGAAGACATCGCCCG           <  SRR4302533.4113399/94‑1 (MQ=60)

                                                                                    TATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGA          >  SRR4302533.2155495/1‑94 (MQ=60)

                                                                                     ATCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGAT         <  SRR4302533.706993/94‑1 (MQ=60)

                                                                                      TCATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATT        >  SRR4302533.2706311/1‑94 (MQ=60)

                                                                                       CATGCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTC       >  SRR4302533.1726437/1‑94 (MQ=60)

                                                                                          GCGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCAAA    <  SRR4302533.1398669/94‑1 (MQ=60)

                                                                                           CGGTACCAGATAATCCCCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACAT   <  SRR4302533.438027/94‑1 (MQ=60)

                                                                                           CGGTACCAGATAATCACCTGCCTTCGCTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACAT   <  SRR4302533.2673622/94‑1 (MQ=60)

                                                                                           CGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACAT   <  SRR4302533.758138/94‑1 (MQ=60)

                                                                                           CGGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACAT   <  SRR4302533.840446/94‑1 (MQ=60)

                                                                                            GGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATA  <  SRR4302533.1166550/94‑1 (MQ=60)

GCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGGGTTTCAACATTTCCCCCTTCCTGCTGGGTAAAGACATCGCCCGATTCACATA  >  NC_000913/2975715‑2975900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`