Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,962,662 A→G M195T (ATG→ACG)  rhlB ← putative ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,962,6620AG100.0% 719.2 / NA 224M195T (ATG→ACG) rhlBputative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (92/132);  total (92/132)

Only 100 of 233 total aligned reads displayed.

TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751
                                                                                          |                                                                                         
tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTg                                                                                         <  2:4267672/93‑1 (MQ=255)
tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTg                                                                                         >  1:5083182/1‑93 (MQ=255)
 gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                        >  1:4245605/1‑93 (MQ=255)
 gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                        <  2:716625/93‑1 (MQ=255)
 gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                        >  2:1429185/1‑93 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGtt                                                                                       >  1:4528849/1‑93 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGtt                                                                                       <  2:3923730/93‑1 (MQ=255)
  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGtt                                                                                       <  2:5573977/93‑1 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      <  2:254102/93‑1 (MQ=255)
   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      <  1:5264135/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  2:5355712/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  2:3088718/93‑1 (MQ=255)
    ccGGTTCCACTTCAATATATTCAGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  1:5280563/93‑1 (MQ=255)
       gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgc                                                                                  <  1:4886249/93‑1 (MQ=255)
       gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgc                                                                                  >  1:4418026/1‑93 (MQ=255)
       gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgc                                                                                  <  1:5779016/93‑1 (MQ=255)
       gTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgc                                                                                  <  1:4147580/93‑1 (MQ=255)
        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  2:2463785/1‑93 (MQ=255)
        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  1:2127681/1‑93 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  2:5982322/93‑1 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  1:4746000/93‑1 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                >  2:1193485/1‑93 (MQ=255)
         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  2:1535580/93‑1 (MQ=255)
           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTg                                                                              >  2:3592188/1‑93 (MQ=255)
            aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                             >  2:1675369/1‑93 (MQ=255)
            aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTgg                                                                             >  1:4388117/1‑93 (MQ=255)
              ttCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGtt                                                                           >  1:3225158/1‑93 (MQ=255)
               tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGttt                                                                          <  2:1981571/93‑1 (MQ=255)
                cAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTg                                                                         <  2:4615098/93‑1 (MQ=255)
                 aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                        >  1:2343993/1‑93 (MQ=255)
                 aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                        <  2:3247395/93‑1 (MQ=255)
                 aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                        >  1:1408609/1‑93 (MQ=255)
                 aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGc                                                                        >  2:806237/1‑93 (MQ=255)
                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       >  1:5422984/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  2:3586673/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      <  2:1853867/93‑1 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  1:1109001/1‑93 (MQ=255)
                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  1:3650060/1‑93 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     >  1:2755163/1‑93 (MQ=255)
                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     <  1:1184954/93‑1 (MQ=255)
                     tatTCGGCATTGTTCATCTGCTCGAACGCCAGGTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGTGGCGCTGGTTTGCAggc                                                                    >  1:6095990/1‑93 (MQ=255)
                        tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcggc                                                                 >  1:3521952/1‑93 (MQ=255)
                        tCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcggc                                                                 >  1:737851/1‑93 (MQ=255)
                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTGCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                <  1:4221387/93‑1 (MQ=255)
                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                >  2:5165053/1‑93 (MQ=255)
                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                <  2:1282465/93‑1 (MQ=255)
                          ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAt                                                               >  1:3914596/1‑93 (MQ=255)
                             agtGTTCATCTGCTCGAACGCCAGTTCACGTACCTGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            <  2:1499088/91‑1 (MQ=255)
                             aTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            >  1:4624858/1‑93 (MQ=255)
                             aTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            <  1:4470573/93‑1 (MQ=255)
                             aTTGTTCACCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            <  2:1245907/93‑1 (MQ=255)
                             aTTGTTCAACTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            >  2:6145478/1‑93 (MQ=255)
                              ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcgg                                                           >  2:374216/1‑93 (MQ=255)
                               tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                          <  2:1045716/93‑1 (MQ=255)
                               tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                          <  2:2209326/93‑1 (MQ=255)
                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  1:6188555/1‑93 (MQ=255)
                                   cATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGGGCTGGTTTGCAGGCGGCATAGGGCGGaa                                                      >  1:813411/1‑93 (MQ=255)
                                   cATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                      <  1:4677225/93‑1 (MQ=255)
                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     <  2:500680/93‑1 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    <  1:1003419/93‑1 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    <  2:1827442/93‑1 (MQ=255)
                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    <  2:5923288/93‑1 (MQ=255)
                                       tGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagc                                                  <  2:5718502/93‑1 (MQ=255)
                                       tGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagc                                                  <  1:985107/93‑1 (MQ=255)
                                       tGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagc                                                  >  1:1130778/1‑93 (MQ=255)
                                        gCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcc                                                 >  1:3494689/1‑93 (MQ=255)
                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  2:4807305/93‑1 (MQ=255)
                                           cGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccagc                                              <  1:3267329/93‑1 (MQ=255)
                                              aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                           >  1:6280057/1‑93 (MQ=255)
                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  2:1903618/93‑1 (MQ=255)
                                                 ccAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGatat                                        >  2:1580155/1‑93 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                       <  1:1580019/93‑1 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                       <  2:2694096/93‑1 (MQ=255)
                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGAGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGCATATc                                       >  1:1531650/1‑93 (MQ=255)
                                                    gTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCtt                                     <  2:542124/93‑1 (MQ=255)
                                                         cGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaata                                <  2:384799/93‑1 (MQ=255)
                                                          gTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaataa                               >  1:6157937/1‑93 (MQ=255)
                                                           tACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                              >  1:5492967/1‑93 (MQ=255)
                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           >  2:5660066/1‑93 (MQ=255)
                                                                 gTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAg                        <  2:2325903/93‑1 (MQ=255)
                                                                  tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                       <  2:277050/93‑1 (MQ=255)
                                                                  tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                       >  1:2833654/1‑93 (MQ=255)
                                                                  tACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGa                       <  2:5798735/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:6084111/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:5860271/93‑1 (MQ=255)
                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:5289552/93‑1 (MQ=255)
                                                                    cGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                     <  2:2083529/93‑1 (MQ=255)
                                                                    cGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                     >  2:746439/1‑93 (MQ=255)
                                                                     gAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCg                    >  1:3322397/1‑93 (MQ=255)
                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   <  1:3159649/93‑1 (MQ=255)
                                                                       aaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTa                  <  1:6030791/93‑1 (MQ=255)
                                                                        aGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTAc                 <  1:1804549/93‑1 (MQ=255)
                                                                           gTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATg              <  2:1730369/93‑1 (MQ=255)
                                                                           gTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATg              >  2:1085539/1‑93 (MQ=255)
                                                                            tGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc             >  1:4077219/1‑93 (MQ=255)
                                                                            tGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc             <  2:2357762/93‑1 (MQ=255)
                                                                            gggCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc             <  2:5123646/92‑1 (MQ=255)
                                                                                ggAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc         <  2:4494281/93‑1 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  >  1:839505/1‑93 (MQ=255)
                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCACATGGCGGAACAGCCGGCGGATATCTTTAATAAAGGCCAGATTGCACATGCTATCGGCtttg  >  2:3035959/1‑91 (MQ=255)
                                                                                          |                                                                                         
TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  >  NC_000913/3962571‑3962755
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CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTG                                                                                             <  SRR4302533.1667023/94‑1 (MQ=60)
CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTG                                                                                             >  SRR4302533.5113904/1‑94 (MQ=60)
CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTG                                                                                             >  SRR4302533.1526702/1‑94 (MQ=60)
 TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGT                                                                                            <  SRR4302533.4264809/94‑1 (MQ=60)
 TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGT                                                                                            >  SRR4302533.4271236/1‑94 (MQ=60)
  GTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTT                                                                                           >  SRR4302533.4556276/1‑94 (MQ=60)
   TTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTG                                                                                          <  SRR4302533.6319402/94‑1 (MQ=60)
    TCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGA                                                                                         <  SRR4302533.5295731/94‑1 (MQ=60)
    TCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGA                                                                                         >  SRR4302533.4877790/1‑94 (MQ=60)
     CCGGTTCCACTTCAATATATTCAGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAG                                                                                        <  SRR4302533.5312205/94‑1 (MQ=60)
       GGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGC                                                                                      >  SRR4302533.4444739/1‑94 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     >  SRR4302533.4217962/1‑94 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     >  SRR4302533.2140801/1‑94 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     <  SRR4302533.4915853/94‑1 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     <  SRR4302533.5813921/94‑1 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     <  SRR4302533.6164177/94‑1 (MQ=60)
        GTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCG                                                                                     <  SRR4302533.4172708/94‑1 (MQ=60)
          TCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCT                                                                                   <  SRR4302533.4774776/94‑1 (MQ=60)
            CACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGG                                                                                 >  SRR4302533.4414609/1‑94 (MQ=60)
             ACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGT                                                                                >  SRR4302533.463935/1‑94 (MQ=60)
              CTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTT                                                                               >  SRR4302533.3245044/1‑94 (MQ=60)
              CTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTT                                                                               >  SRR4302533.3086934/1‑94 (MQ=60)
              CTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTT                                                                               <  SRR4302533.776225/94‑1 (MQ=60)
              CTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTT                                                                               >  SRR4302533.819113/1‑94 (MQ=60)
               TTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTT                                                                              <  SRR4302533.1097749/94‑1 (MQ=60)
                TCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTG                                                                             <  SRR4302533.6154686/94‑1 (MQ=60)
                 CAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGC                                                                            >  SRR4302533.1417123/1‑94 (MQ=60)
                 CAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGC                                                                            >  SRR4302533.2358502/1‑94 (MQ=60)
                  AATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCA                                                                           >  SRR4302533.5455617/1‑94 (MQ=60)
                  AATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCA                                                                           <  SRR4302533.5139532/94‑1 (MQ=60)
                   ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302533.937906/1‑94 (MQ=60)
                   ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          <  SRR4302533.5025602/94‑1 (MQ=60)
                   ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302533.3672505/1‑94 (MQ=60)
                   ATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAG                                                                          >  SRR4302533.1115584/1‑94 (MQ=60)
                    TATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGG                                                                         >  SRR4302533.2772058/1‑94 (MQ=60)
                     ATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGC                                                                        >  SRR4302533.3934511/1‑94 (MQ=60)
                     ATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGC                                                                        <  SRR4302533.1192066/94‑1 (MQ=60)
                     ATATTCGGCATTGTTCATCTGCTCGAACGCCAGGTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGTGGCGCTGGTTTGCAGGC                                                                        >  SRR4302533.6132814/1‑94 (MQ=60)
                      TATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCG                                                                       <  SRR4302533.4861256/94‑1 (MQ=60)
                        TTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGC                                                                     >  SRR4302533.3543661/1‑94 (MQ=60)
                        TTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGC                                                                     >  SRR4302533.742195/1‑94 (MQ=60)
                          CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTGCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAT                                                                   <  SRR4302533.4246914/94‑1 (MQ=60)
                          CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAT                                                                   >  SRR4302533.2319870/1‑94 (MQ=60)
                          CGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAT                                                                   >  SRR4302533.3938508/1‑94 (MQ=60)
                           GGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACTAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATA                                                                  <  SRR4302533.3633399/94‑1 (MQ=60)
                           GGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATA                                                                  <  SRR4302533.3672932/94‑1 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                >  SRR4302533.4652892/1‑94 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302533.182215/94‑1 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                >  SRR4302533.1247140/1‑94 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                >  SRR4302533.685780/1‑94 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302533.3292381/94‑1 (MQ=60)
                             CATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACG                                                                <  SRR4302533.124563/94‑1 (MQ=60)
                              ATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGG                                                               <  SRR4302533.4497610/94‑1 (MQ=60)
                              ATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGG                                                               >  SRR4302533.1606142/1‑94 (MQ=60)
                                TGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCG                                                             >  SRR4302533.4652131/1‑94 (MQ=60)
                                TGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCG                                                             <  SRR4302533.442880/94‑1 (MQ=60)
                                TGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCG                                                             >  SRR4302533.6225695/1‑94 (MQ=60)
                                   TCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGGGCTGGTTTGCAGGCGGCATAGGGCGGAA                                                          >  SRR4302533.818171/1‑94 (MQ=60)
                                   TCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAA                                                          >  SRR4302533.5757315/1‑94 (MQ=60)
                                    CATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGTAGGCGGCATACGGCGGAAC                                                         >  SRR4302533.3966088/1‑94 (MQ=60)
                                    CATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAC                                                         <  SRR4302533.4705571/94‑1 (MQ=60)
                                     ATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACA                                                        >  SRR4302533.1138967/1‑94 (MQ=60)
                                     ATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACA                                                        <  SRR4302533.4523040/94‑1 (MQ=60)
                                     ATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACA                                                        >  SRR4302533.3471828/1‑94 (MQ=60)
                                     ATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGGTGAGGCGCTGGGTTGCAGGCGGGCTACGGCGGGACA                                                        >  SRR4302533.5807194/1‑94 (MQ=60)
                                      TCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAG                                                       <  SRR4302533.6117314/94‑1 (MQ=60)
                                      TCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAG                                                       <  SRR4302533.1009283/94‑1 (MQ=60)
                                       CTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGC                                                      >  SRR4302533.1137498/1‑94 (MQ=60)
                                        TGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCC                                                     <  SRR4302533.990876/94‑1 (MQ=60)
                                        TGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCC                                                     >  SRR4302533.3516243/1‑94 (MQ=60)
                                        TGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCC                                                     >  SRR4302533.2455199/1‑94 (MQ=60)
                                         GCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCA                                                    <  SRR4302533.6232182/94‑1 (MQ=60)
                                           TCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGC                                                  <  SRR4302533.1133467/94‑1 (MQ=60)
                                            CGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCG                                                 >  SRR4302533.1555836/1‑94 (MQ=60)
                                            CGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCG                                                 <  SRR4302533.3287493/94‑1 (MQ=60)
                                            CGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCG                                                 >  SRR4302533.5301251/1‑94 (MQ=60)
                                             GAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGG                                                >  SRR4302533.4470563/1‑94 (MQ=60)
                                              AACGGCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGA                                               <  SRR4302533.4223915/94‑1 (MQ=60)
                                              AACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGA                                               >  SRR4302533.6317544/1‑94 (MQ=60)
                                                 GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            >  SRR4302533.354338/1‑94 (MQ=60)
                                                 GCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATAT                                            <  SRR4302533.5487484/94‑1 (MQ=60)
                                                  CCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGAGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGCATATC                                           >  SRR4302533.1540854/1‑94 (MQ=60)
                                                   CAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCT                                          >  SRR4302533.1626304/1‑94 (MQ=60)
                                                   CAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCT                                          <  SRR4302533.867809/94‑1 (MQ=60)
                                                   CAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCT                                          <  SRR4302533.1589537/94‑1 (MQ=60)
                                                      TTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTA                                       <  SRR4302533.3183446/94‑1 (MQ=60)
                                                          CGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAA                                   >  SRR4302533.6208017/1‑94 (MQ=60)
                                                          CGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAA                                   >  SRR4302533.6194977/1‑94 (MQ=60)
                                                          CGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAA                                   >  SRR4302533.3186679/1‑94 (MQ=60)
                                                           GTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAA                                  >  SRR4302533.5526099/1‑94 (MQ=60)
                                                            TACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAG                                 <  SRR4302533.4426696/94‑1 (MQ=60)
                                                                 GGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAAAGCCAGCGGATATCTTTAATAAAGCACAG                            >  SRR4302533.3356646/1‑94 (MQ=60)
                                                                  GTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGA                           >  SRR4302533.4703499/1‑94 (MQ=60)
                                                                  GTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGA                           >  SRR4302533.2851099/1‑94 (MQ=60)
                                                                     CGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCG                        <  SRR4302533.4143600/94‑1 (MQ=60)
                                                                     CGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCG                        >  SRR4302533.3342915/1‑94 (MQ=60)
                                                                     CGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCG                        <  SRR4302533.4722887/94‑1 (MQ=60)
                                                                       AAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTA                      <  SRR4302533.3179093/94‑1 (MQ=60)
                                                                        AAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTAC                     <  SRR4302533.6067339/94‑1 (MQ=60)
                                                                        AAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTAC                     >  SRR4302533.604055/1‑94 (MQ=60)
                                                                         AGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACA                    <  SRR4302533.1815533/94‑1 (MQ=60)
                                                                          GCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAT                   <  SRR4302533.5308772/94‑1 (MQ=60)
                                                                            GTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGC                 >  SRR4302533.4102040/1‑94 (MQ=60)
                                                                                 GGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCG            <  SRR4302533.5421018/94‑1 (MQ=60)
                                                                                  GAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGG           <  SRR4302533.284852/94‑1 (MQ=60)
                                                                                      ACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTC       <  SRR4302533.4340804/94‑1 (MQ=60)
                                                                                      ACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTC       <  SRR4302533.3287383/94‑1 (MQ=60)
                                                                                       CAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG      >  SRR4302533.844418/1‑94 (MQ=60)
                                                                                        AGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTAAATGGGATCGGCTTCGT     >  SRR4302533.577883/1‑94 (MQ=60)
                                                                                          CGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCC   >  SRR4302533.2695392/1‑94 (MQ=60)
                                                                                           GTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  >  SRR4302533.3134128/1‑94 (MQ=60)
                                                                                           |                                                                                             
CTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCA  >  NC_000913/3962571‑3962755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑